Sed0012560 (gene) Chayote v1

Overview
NameSed0012560
Typegene
OrganismSechium edule (Chayote v1)
DescriptionProtein kinase domain-containing protein
LocationLG12: 28956036 .. 28957610 (+)
RNA-Seq ExpressionSed0012560
SyntenySed0012560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTGTCTTGCTCTCGAAGTTACCGGAAAAGTGGTCCCCATCTTCTGGTTTTTCAAGCAACCGTTCCTCTCCGGCCACCGCACCTCCGTTCCACGGCGGTTCCGGCGGTTCAAGAATTTCAGTCAATTTCAGATTCGTCTTCTTTTTCAGTGGATTCAACATTGTCTCTAATATTTCCAGGTTCGTAGTCCAACTTCTCCGTATTCATATTCTTTTTTTCATAATTAGATTAGCATCTGAATTAGGTGAAAATTTCCCTGTTCTAATCGAAGTATTGTAGAAATTAATTAACTCAGAGTGATGAGTCTTAAGTTTTGTTCATAATAATTTTGAATGCCGTTAGGGTTTAGAGTTTCTGTTAATTTCAAAATCAAACCTGTTAATTAAATAATAATATTGTTTTTTTGCTTTTAATTTTTGACAGGTTTGATTTATGATATATAAAGTATGACAAAATCCTGTGTTGTAATCTCCAAAAGTCATCACTATATGAATTTGCACGAATTTTTGAAAACAAAAAGGAGAATTGTGAAATATGCGAAGAAGACATTACCAAACAACAATTGGAACGAAATCTTTGATCCTTTGTCTGTTAAGGAATTACACGGTTCGTTTTGGGTTGGAGATTGTTTATTTCGTAAAAGGGGCTCTGAAATTATATTTTTGGCGAAGAACAAAGAATCCACAGCCCCAAATTCAAAATTCCCCAAAGAAATTATTGTCAAATCTTGCCCTCTAGAACTCTCTTCTTCACTCCAACATGAAAAGGATTTGCTTTTCAAATTTGCACATTGCAAGAACGTGGTGCATTGCTATGGAGATGAAATTACGACCACGAATTCGGGCCGAAAGATTTACAATGTGTTATTGGAGTATTGCTCTGGCTCTTGCCTGCGTGACCATATGATTAACTATGGACTTTACGGGCTGCCCGAATTTGAAGCTCGTCGGTATACGAGAGACATTGTGCACGGGCTCTATCACATGCATTGCAATGCTCGGTACATCCACGGCGACATCAAACCACGAAACATCCTATTGTTACCGAATGACGACGACGGAGATTCTTTTGTGGCTAAGATAGCGAGTTTTGGATTGGCTAAGAAGTTGACTCCGGATGTAGTATGCGATCACGGGATTTGTGGTTCGGGACCGTACACCTCTCCGGAGGTGGTGAGCGATGGTTGCTTGGATTGGCCTGCTGATGTTTGGGCACTTGGATGTGTGGTGTTGGAGATGTTGATGGGAAAATGGAGTTTTGAAAATGCTTATGAGTGTGGTGAGTTGCCACAAATTCCAAAGGAGATTTCAAGTGTTGGAAGAGATTTTATAAAGAAGTGTTTGGTTAGAAGTCCTTACAAGAGAAGACCTGTTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTGTGAAGAACTCATATGTTGAATGAAGCCAATGGTGTTGTGTTTTGTAAACAGTTGTTTAGTAATATTTTAGGAGTTTTGAGTTAATGTTTTAATGTAACTTTTTTGTTACAGATGTTTTGTAAATCTCATCCAATATTTCTATATTTTTTATTAATACAACGATATTAC

mRNA sequence

TCTTGTCTTGCTCTCGAAGTTACCGGAAAAGTGGTCCCCATCTTCTGGTTTTTCAAGCAACCGTTCCTCTCCGGCCACCGCACCTCCGTTCCACGGCGGTTCCGGCGGTTCAAGAATTTCAGTCAATTTCAGATTCGTCTTCTTTTTCAGTGGATTCAACATTGTCTCTAATATTTCCAGGTTTGATTTATGATATATAAAGTATGACAAAATCCTGTGTTGTAATCTCCAAAAGTCATCACTATATGAATTTGCACGAATTTTTGAAAACAAAAAGGAGAATTGTGAAATATGCGAAGAAGACATTACCAAACAACAATTGGAACGAAATCTTTGATCCTTTGTCTGTTAAGGAATTACACGGTTCGTTTTGGGTTGGAGATTGTTTATTTCGTAAAAGGGGCTCTGAAATTATATTTTTGGCGAAGAACAAAGAATCCACAGCCCCAAATTCAAAATTCCCCAAAGAAATTATTGTCAAATCTTGCCCTCTAGAACTCTCTTCTTCACTCCAACATGAAAAGGATTTGCTTTTCAAATTTGCACATTGCAAGAACGTGGTGCATTGCTATGGAGATGAAATTACGACCACGAATTCGGGCCGAAAGATTTACAATGTGTTATTGGAGTATTGCTCTGGCTCTTGCCTGCGTGACCATATGATTAACTATGGACTTTACGGGCTGCCCGAATTTGAAGCTCGTCGGTATACGAGAGACATTGTGCACGGGCTCTATCACATGCATTGCAATGCTCGGTACATCCACGGCGACATCAAACCACGAAACATCCTATTGTTACCGAATGACGACGACGGAGATTCTTTTGTGGCTAAGATAGCGAGTTTTGGATTGGCTAAGAAGTTGACTCCGGATGTAGTATGCGATCACGGGATTTGTGGTTCGGGACCGTACACCTCTCCGGAGGTGGTGAGCGATGGTTGCTTGGATTGGCCTGCTGATGTTTGGGCACTTGGATGTGTGGTGTTGGAGATGTTGATGGGAAAATGGAGTTTTGAAAATGCTTATGAGTGTGGTGAGTTGCCACAAATTCCAAAGGAGATTTCAAGTGTTGGAAGAGATTTTATAAAGAAGTGTTTGGTTAGAAGTCCTTACAAGAGAAGACCTGTTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTGTGAAGAACTCATATGTTGAATGAAGCCAATGGTGTTGTGTTTTGTAAACAGTTGTTTAGTAATATTTTAGGAGTTTTGAGTTAATGTTTTAATGTAACTTTTTTGTTACAGATGTTTTGTAAATCTCATCCAATATTTCTATATTTTTTATTAATACAACGATATTAC

Coding sequence (CDS)

ATGACAAAATCCTGTGTTGTAATCTCCAAAAGTCATCACTATATGAATTTGCACGAATTTTTGAAAACAAAAAGGAGAATTGTGAAATATGCGAAGAAGACATTACCAAACAACAATTGGAACGAAATCTTTGATCCTTTGTCTGTTAAGGAATTACACGGTTCGTTTTGGGTTGGAGATTGTTTATTTCGTAAAAGGGGCTCTGAAATTATATTTTTGGCGAAGAACAAAGAATCCACAGCCCCAAATTCAAAATTCCCCAAAGAAATTATTGTCAAATCTTGCCCTCTAGAACTCTCTTCTTCACTCCAACATGAAAAGGATTTGCTTTTCAAATTTGCACATTGCAAGAACGTGGTGCATTGCTATGGAGATGAAATTACGACCACGAATTCGGGCCGAAAGATTTACAATGTGTTATTGGAGTATTGCTCTGGCTCTTGCCTGCGTGACCATATGATTAACTATGGACTTTACGGGCTGCCCGAATTTGAAGCTCGTCGGTATACGAGAGACATTGTGCACGGGCTCTATCACATGCATTGCAATGCTCGGTACATCCACGGCGACATCAAACCACGAAACATCCTATTGTTACCGAATGACGACGACGGAGATTCTTTTGTGGCTAAGATAGCGAGTTTTGGATTGGCTAAGAAGTTGACTCCGGATGTAGTATGCGATCACGGGATTTGTGGTTCGGGACCGTACACCTCTCCGGAGGTGGTGAGCGATGGTTGCTTGGATTGGCCTGCTGATGTTTGGGCACTTGGATGTGTGGTGTTGGAGATGTTGATGGGAAAATGGAGTTTTGAAAATGCTTATGAGTGTGGTGAGTTGCCACAAATTCCAAAGGAGATTTCAAGTGTTGGAAGAGATTTTATAAAGAAGTGTTTGGTTAGAAGTCCTTACAAGAGAAGACCTGTTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTGTGA

Protein sequence

MTKSCVVISKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNEIFDPLSVKELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGKWSFENAYECGELPQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVCL
Homology
BLAST of Sed0012560 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.4 bits (1095), Expect = 2.2e-115
Identity = 208/335 (62.09%), Postives = 249/335 (74.33%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE-------IFDPLSVK 60
           MTKSCVV     + H+ +NLHEFLKTKR IVK AKK+  +NNWN         F   +++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60

Query: 61  ELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           E HGSFW+GD L RK GS ++FLAKNK ST  N +F    +VKSC +E SSSLQ+EK++L
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEF----LVKSCLMESSSSLQYEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                C N+V CYGDEIT   SG +IYNV+ EYC GS LRDH+I +G  GLPE   R+YT
Sbjct: 121 RNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIVHGL +MHCNA YIHGDIKPRNILL P+D  G   VAK+ASFGLA+KLTP+V+CD  
Sbjct: 181 RDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG-ELPQI 300
           I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+AY    +C  E+P+I
Sbjct: 241 ISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEI 300

Query: 301 PKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           P +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 301 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of Sed0012560 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.5 bits (1085), Expect = 3.1e-114
Identity = 207/335 (61.79%), Postives = 247/335 (73.73%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE-------IFDPLSVK 60
           MTKSCVV     + H+ +NLHEFLKTKR IVK AKK+  + NWN         F   +++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           E HGSFW+GD L RK GS ++FLAKNK ST  NS+F    +VKSC +E SSSLQ+EK++L
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNSEF----LVKSCLMESSSSLQYEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                C N+V CYGDEIT   SG +IYNV+ EYC GS LRDH+I +G  GLPE   R+YT
Sbjct: 121 CNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIV GL +MHCNA YIHGDIKPRNILL P+D  G   VAK+ASFGLA+KLTP+V+CD  
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG-ELPQI 300
           I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+AY     C  E+P+I
Sbjct: 241 ISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEI 300

Query: 301 PKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           P +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 301 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of Sed0012560 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 421.8 bits (1083), Expect = 5.4e-114
Identity = 206/335 (61.49%), Postives = 248/335 (74.03%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE-------IFDPLSVK 60
           MTKSCVV     + H+ +NLHEFLKTKR IVK AKK+  + NWN         F   +++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           E HGSFW+GD L R+ GS ++FLAKNK ST  NS+F    +VKSC +E SSSLQ+EK++L
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEF----LVKSCLMESSSSLQYEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                C N+V CYGDEIT + SG +IYNV+ EYC GS LRDH+I +G  GLPE   R+YT
Sbjct: 121 CNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIV GL +MHCNA YIHGDIKPRNILL P+D  G   VAK+ASFGLA+KLTP+V+CD  
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG-ELPQI 300
           I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+AY     C  E+P+I
Sbjct: 241 ISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEI 300

Query: 301 PKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           P +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 301 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of Sed0012560 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-113
Identity = 206/335 (61.49%), Postives = 247/335 (73.73%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE-------IFDPLSVK 60
           MTKSCVV     + H+ +NLHEFLKTKR IVK AKK+  + NWN         F   +++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           E HGSFW+GD L RK GS ++FLAKNK ST  NS+F    +VKSC +E SSSLQ+EK++L
Sbjct: 61  ECHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNSEF----LVKSCLMESSSSLQYEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                C N+V CYGDEIT   SG +IYNV+ EYC GS LRDH+I +G  GLPE   R+YT
Sbjct: 121 CNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIV GL ++HCNA YIHGDIKPRNILL P+D  G   VAK+ASFGLA+KLTP+V+CD  
Sbjct: 181 RDIVRGLCYIHCNAGYIHGDIKPRNILLSPSDRKGGCLVAKLASFGLARKLTPEVLCDEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG-ELPQI 300
           I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+AY     C  E+P+I
Sbjct: 241 ISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEI 300

Query: 301 PKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           P +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 301 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of Sed0012560 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 402.9 bits (1034), Expect = 2.6e-108
Identity = 200/331 (60.42%), Postives = 235/331 (71.00%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE--------IFDPLSV 60
           MTKSCVV     +SH+++NLHEFLKTKR IVK AKK+L  NNWN+             ++
Sbjct: 1   MTKSCVVSQVNQRSHNFVNLHEFLKTKRGIVKCAKKSLRTNNWNDNVKHNSEKFGKDFAI 60

Query: 61  KELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDL 120
           +E HGS W GD LFRK GS +IFLA+N+  T PNS  P E +VKSC +E SSSLQHEK++
Sbjct: 61  EEYHGSIWTGDHLFRKTGSSLIFLARNRAPTNPNSNLPTEFMVKSCLMESSSSLQHEKEV 120

Query: 121 LFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRY 180
           L       N+V CYGDEIT T SG K+YNVL EYC GS LRDH++ +G  GLPE E RRY
Sbjct: 121 LNNLGSFTNLVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHEVRRY 180

Query: 181 TRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDH 240
           TRDIV+G+ +MHCN RYIHGDIK RNIL+ P         AK+ASFG A+KLT +V+C+ 
Sbjct: 181 TRDIVYGVCYMHCNGRYIHGDIKSRNILMSPTG-------AKLASFGWARKLTAEVLCEE 240

Query: 241 GICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGKWSFENAYECGELPQIPKEISS 300
            I GSGPY SPE+ SDG L WPAD+WALGCVVLEML GK    N     E  QI  +IS 
Sbjct: 241 EIRGSGPYISPELASDGYLGWPADIWALGCVVLEMLTGKSPRNNE----EAIQIINDISK 300

Query: 301 VGRDFIKKCLVRSPYKRRPVWLLIKHPFVCL 321
            GRDFIKKCL+ SPYKRRP WLLIKHPFVC+
Sbjct: 301 EGRDFIKKCLITSPYKRRPSWLLIKHPFVCV 320

BLAST of Sed0012560 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-39
Identity = 105/274 (38.32%), Postives = 143/274 (52.19%), Query Frame = 0

Query: 66  RGSEIIFLAKNKESTAPNSK----FPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVH 125
           RG  I F   +  STA  S+    FP  I VKS     ++SL +EK +L     C  ++ 
Sbjct: 5   RGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIR 64

Query: 126 CYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMH 185
           CYG++ T  N G +++N+LLEY S   L  +M   G  GLPE   RR+T  ++ GL H+H
Sbjct: 65  CYGEDSTVEN-GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIH 124

Query: 186 CNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVC---DHGICGSGPYT 245
               + H DIK  NILL    +DG     KIA FGLA ++  D+        I G+  Y 
Sbjct: 125 AKG-FAHCDIKLANILLF---NDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTPLYM 184

Query: 246 SPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAYEC----------GELPQIPKE 305
           +PE V+D      ADVWALGC V+EM  GK  WS +                ELP+IP+ 
Sbjct: 185 APECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEM 244

Query: 306 ISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVCL 321
           +S  G+DF+ KC V+ P KR    +L+ H FV +
Sbjct: 245 LSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

BLAST of Sed0012560 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-23
Identity = 83/232 (35.78%), Postives = 119/232 (51.29%), Query Frame = 0

Query: 98  ELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYG 157
           E +  L  E  +L +  H +N+V  YG E        K+Y + LEY SG  +   +  YG
Sbjct: 442 ESAQQLGQEISVLSRLRH-QNIVQYYGSETVDD----KLY-IYLEYVSGGSIYKLLQEYG 501

Query: 158 LYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGL 217
            +G  E   R YT+ I+ GL ++H     +H DIK  NIL+ P+         K+A FG+
Sbjct: 502 QFG--ENAIRNYTQQILSGLAYLHAK-NTVHRDIKGANILVDPHGR------VKVADFGM 561

Query: 218 AKKLTPDVVCDHGICGSGPYTSPEVV--SDGCLDWPADVWALGCVVLEMLMGK--WS-FE 277
           AK +T          GS  + +PEV+  S+G  +   D+W+LGC VLEM   K  WS +E
Sbjct: 562 AKHITAQ-SGPLSFKGSPYWMAPEVIKNSNGS-NLAVDIWSLGCTVLEMATTKPPWSQYE 621

Query: 278 NA---YECG---ELPQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
                ++ G   ELP IP  +S  G+DF++KCL R+P  R     L+ H FV
Sbjct: 622 GVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of Sed0012560 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 74/245 (30.20%), Postives = 115/245 (46.94%), Query Frame = 0

Query: 90  IIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEITT----TNSGRKIYNVLLEYCS 149
           + VKS     S  LQ E  +L    +   V+   G EIT      N     Y++L+EY  
Sbjct: 29  LAVKSAEFHRSEFLQREAKILSSL-NSPYVIGYRGCEITREPFHNNGEATTYSLLMEYAP 88

Query: 150 GSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDG 209
              L D     G + + E    +YTR I+ GL ++H +    H DIK  N+L+      G
Sbjct: 89  YGTLTDVATKNGGF-IDEARVVKYTRQILLGLEYIHNSKGIAHCDIKGSNVLV------G 148

Query: 210 DSFVAKIASFGLAKKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEML 269
           ++  AKIA FG AK + P++     + G+  + +PE          +D+WA+GC V+EM+
Sbjct: 149 ENGEAKIADFGCAKWVEPEIT--EPVRGTPAFMAPEAARGERQGKESDIWAVGCTVIEMV 208

Query: 270 MGKWSFENA---------YE---CGELPQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLI 319
            G   +  A         Y     GELP++P  ++   +DF+ KCL +   +R     L+
Sbjct: 209 TGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFLGKCLKKEATERWTASQLL 263

BLAST of Sed0012560 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.2e-21
Identity = 83/307 (27.04%), Postives = 140/307 (45.60%), Query Frame = 0

Query: 25  RRIVKYAK-KTLPNNNWNEIFDPLSVKELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPN 84
           R+ + +AK ++ PNN+  +I  P+         W    L  +     +++  N +S    
Sbjct: 43  RKSMVFAKSQSPPNNSTVQIKPPIR--------WRKGQLIGRGAFGTVYMGMNLDS--GE 102

Query: 85  SKFPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEY 144
               K++++ S       +  H ++L  +    KN+ H        T    +  N+LLE+
Sbjct: 103 LLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEF 162

Query: 145 CSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDD 204
             G  +   +  +G +  PE   R YT  ++ GL ++H N   +H DIK  NIL+   D+
Sbjct: 163 VPGGSISSLLEKFGAF--PESVVRTYTNQLLLGLEYLH-NHAIMHRDIKGANILV---DN 222

Query: 205 DGDSFVAKIASFGLAKKLT--PDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALGCVV 264
            G     K+A FG +K++     +     + G+  + +PEV+      + AD+W++GC V
Sbjct: 223 QG---CIKLADFGASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTV 282

Query: 265 LEMLMGKWSFENAYE----------CGELPQIPKEISSVGRDFIKKCLVRSPYKRRPVWL 319
           +EM+ GK  +   Y+              P IP  ISS   DF+ KCL + P  R     
Sbjct: 283 IEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASE 330

BLAST of Sed0012560 vs. ExPASy Swiss-Prot
Match: F4JBP3 (Serine/threonine-protein kinase ATG1b OS=Arabidopsis thaliana OX=3702 GN=ATG1B PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-19
Identity = 73/242 (30.17%), Postives = 120/242 (49.59%), Query Frame = 0

Query: 88  KEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGS 147
           KEI +     +L  SL  E  +L K  H  N++      I    +  KI N++LEYC G 
Sbjct: 49  KEIAMARLNKKLQESLMSEIIILRKINH-PNIIRF----IDMIEAPGKI-NLVLEYCKGG 108

Query: 148 CLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDS 207
            L  ++  +G   +PE  A+ +   +  GL  +  N   IH D+KP+N+LL  +D+D   
Sbjct: 109 DLSMYIHKHG--SVPEATAKHFMLQLAAGLQVLRDN-NIIHRDLKPQNLLLSTDDNDA-- 168

Query: 208 FVAKIASFGLAKKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMG 267
              KIA FG A+ L P  + +  +CGS  Y +PE++     D  AD+W++G ++ +++ G
Sbjct: 169 -ALKIADFGFARSLQPRGLAE-TLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTG 228

Query: 268 KWSF---------ENAYECGEL--PQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHP 319
           +  F         +N     EL  P   +++S+  +D  +K L R+P +R        HP
Sbjct: 229 RTPFTGNSQIQLLQNIIRSTELHFPADCRDLSTDCKDLCQKLLRRNPVERLTFEEFFHHP 277

BLAST of Sed0012560 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.6e-114
Identity = 206/335 (61.49%), Postives = 248/335 (74.03%), Query Frame = 0

Query: 1   MTKSCVVI---SKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNE-------IFDPLSVK 60
           MTKSCVV     + H+ +NLHEFLKTKR IVK AKK+  + NWN         F   +++
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ELHGSFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           E HGSFW+GD L R+ GS ++FLAKNK ST  NS+F    +VKSC +E SSSLQ+EK++L
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEF----LVKSCLMESSSSLQYEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                C N+V CYGDEIT + SG +IYNV+ EYC GS LRDH+I +G  GLPE   R+YT
Sbjct: 121 CNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIV GL +MHCNA YIHGDIKPRNILL P+D  G   VAK+ASFGLA+KLTP+V+CD  
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG-ELPQI 300
           I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+AY     C  E+P+I
Sbjct: 241 ISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEI 300

Query: 301 PKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           P +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 301 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

BLAST of Sed0012560 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 9.9e-106
Identity = 195/337 (57.86%), Postives = 236/337 (70.03%), Query Frame = 0

Query: 1   MTKSCVVISKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNEIF--------DPLSVKEL 60
           MTKSCVV S+     NLHEFLKTKRRI+K AKK+ P NNWN+          +  +++E 
Sbjct: 1   MTKSCVV-SQVQISGNLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTIEEF 60

Query: 61  HGSFWVGDCLFRKRGSEIIFLAKNKES--TAPNSKFPKEIIVKSCPLELSSSLQHEKDLL 120
           HGS W G  LFRK  S ++FLAK   +     NS  P+E +VKS  +E SSSL+HEK++L
Sbjct: 61  HGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                  N+V CYGDEIT T SG ++YNV  EYCSGS LR+H++ +G  GL + E RRYT
Sbjct: 121 SNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVRRYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIV GLY+MHCN RYIHGDIK RNILL          +AK+ASFGLA+KLT +V+C+  
Sbjct: 181 RDIVRGLYYMHCNGRYIHGDIKSRNILL-------SHGMAKLASFGLARKLTAEVICEEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAYEC------GELPQ 300
           I GSGPY SPE+  +G L WPAD+WALGCVVLEM  GK  WSFE+AY         ++P+
Sbjct: 241 ISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPE 300

Query: 301 IPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVC 320
           IPK IS  GRDFIKKCL+RSPYKRRP+WLLIKHPFVC
Sbjct: 301 IPKNISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329

BLAST of Sed0012560 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 8.4e-105
Identity = 197/337 (58.46%), Postives = 235/337 (69.73%), Query Frame = 0

Query: 1   MTKSCVVISKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNEIF--------DPLSVKEL 60
           MTKSCVV S+    +NLHEFLKTKRRI+K AKK+ P NNWN+          +  +++E 
Sbjct: 1   MTKSCVV-SRVQFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEF 60

Query: 61  HGSFWVGDCLFRKRGSEIIFLAKNKESTAPN--SKFPKEIIVKSCPLELSSSLQHEKDLL 120
           HGS W G  LFRK  S +IFLAK   +   N  S  P+E +VKS   E SSSL HEK++L
Sbjct: 61  HGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                  N+V CYGDEIT + SG K+YNV  EYCSGS LRDH+  +G  GL E E R+YT
Sbjct: 121 SNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIVHGLY+MHCN RYIHGDIK RNILL          +AK+ASFGLA+KLT +V+C+  
Sbjct: 181 RDIVHGLYYMHCNGRYIHGDIKSRNILL-------SHGMAKLASFGLARKLTAEVICEEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG--ELPQ 300
           I GSGPY SPE+  +G L WPAD+WALGCVVLEM  GK  WSF +A+    +C   ++P+
Sbjct: 241 ISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPE 300

Query: 301 IPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVC 320
           IPK IS  GRDFIKKCL+RSPYKRRP WLLIKHPFVC
Sbjct: 301 IPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

BLAST of Sed0012560 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 8.4e-105
Identity = 197/337 (58.46%), Postives = 235/337 (69.73%), Query Frame = 0

Query: 1   MTKSCVVISKSHHYMNLHEFLKTKRRIVKYAKKTLPNNNWNEIF--------DPLSVKEL 60
           MTKSCVV S+    +NLHEFLKTKRRI+K AKK+ P NNWN+          +  +++E 
Sbjct: 1   MTKSCVV-SRVQFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEF 60

Query: 61  HGSFWVGDCLFRKRGSEIIFLAKNKESTAPN--SKFPKEIIVKSCPLELSSSLQHEKDLL 120
           HGS W G  LFRK  S +IFLAK   +   N  S  P+E +VKS   E SSSL HEK++L
Sbjct: 61  HGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVL 120

Query: 121 FKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYT 180
                  N+V CYGDEIT + SG K+YNV  EYCSGS LRDH+  +G  GL E E R+YT
Sbjct: 121 SNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYT 180

Query: 181 RDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG 240
           RDIVHGLY+MHCN RYIHGDIK RNILL          +AK+ASFGLA+KLT +V+C+  
Sbjct: 181 RDIVHGLYYMHCNGRYIHGDIKSRNILL-------SHGMAKLASFGLARKLTAEVICEEE 240

Query: 241 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAY----ECG--ELPQ 300
           I GSGPY SPE+  +G L WPAD+WALGCVVLEM  GK  WSF +A+    +C   ++P+
Sbjct: 241 ISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPE 300

Query: 301 IPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVC 320
           IPK IS  GRDFIKKCL+RSPYKRRP WLLIKHPFVC
Sbjct: 301 IPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

BLAST of Sed0012560 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.5e-87
Identity = 153/229 (66.81%), Postives = 181/229 (79.04%), Query Frame = 0

Query: 97  LELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINY 156
           +E SSSLQ+EK++L     C N+V CYGDEIT   SG +IYNV+ EYC GS LRDH+I +
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 157 GLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFG 216
           G  GLPE   R+YTRDIVHGL +MHCNARYIHGDIKPRNILL P+D  G   VAK+ASFG
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFG 120

Query: 217 LAKKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENA 276
           LA+KLTP+V+CD  I GSG Y +PE+  DG LDWPAD+WALGCVVLEML GK  WSFE+A
Sbjct: 121 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 180

Query: 277 Y----ECG-ELPQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFV 319
           Y    +C  E+P+IP +ISS GRDF+K+CL+RSPYKRRP WLL+KHPFV
Sbjct: 181 YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 229

BLAST of Sed0012560 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 173.3 bits (438), Expect = 3.1e-43
Identity = 101/256 (39.45%), Postives = 147/256 (57.42%), Query Frame = 0

Query: 79  STAPNSKFPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEITTTNSGRKIYN 138
           S + +  FP  + VKS  +  S++L++E+D+L     C  +V C+G+  T  N G +IYN
Sbjct: 23  SGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFGEGRTVEN-GEEIYN 82

Query: 139 VLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNILL 198
           + LEY SG  L D + + G   LPEFE RR+TR IV GL H+H N  + H DIK  N+L+
Sbjct: 83  LFLEYASGGSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG-FTHCDIKLENVLV 142

Query: 199 LPNDDDGDSFVAKIASFGLAKKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPADVWALG 258
                DGD    KI+ FGLAK+ + +V  +  I G+  Y +PE V+ G  + PAD+WALG
Sbjct: 143 F---GDGD---VKISDFGLAKRRSGEVCVE--IRGTPLYMAPESVNHGEFESPADIWALG 202

Query: 259 CVVLEMLMGK--WSFENAY------------ECGELPQIPKEISSVGRDFIKKCLVRSPY 318
           C V+EM  GK  W  E+                 E+P+IP E+S  G+DF+ KC V++  
Sbjct: 203 CSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAA 262

Query: 319 KRRPVWLLIKHPFVCL 321
           +R    +L+ HPF+ +
Sbjct: 263 ERWTAEMLLDHPFLAV 267

BLAST of Sed0012560 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 165.2 bits (417), Expect = 8.4e-41
Identity = 105/274 (38.32%), Postives = 143/274 (52.19%), Query Frame = 0

Query: 66  RGSEIIFLAKNKESTAPNSK----FPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVH 125
           RG  I F   +  STA  S+    FP  I VKS     ++SL +EK +L     C  ++ 
Sbjct: 5   RGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIR 64

Query: 126 CYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMH 185
           CYG++ T  N G +++N+LLEY S   L  +M   G  GLPE   RR+T  ++ GL H+H
Sbjct: 65  CYGEDSTVEN-GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIH 124

Query: 186 CNARYIHGDIKPRNILLLPNDDDGDSFVAKIASFGLAKKLTPDVVC---DHGICGSGPYT 245
               + H DIK  NILL    +DG     KIA FGLA ++  D+        I G+  Y 
Sbjct: 125 AKG-FAHCDIKLANILLF---NDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTPLYM 184

Query: 246 SPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAYEC----------GELPQIPKE 305
           +PE V+D      ADVWALGC V+EM  GK  WS +                ELP+IP+ 
Sbjct: 185 APECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEM 244

Query: 306 ISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVCL 321
           +S  G+DF+ KC V+ P KR    +L+ H FV +
Sbjct: 245 LSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

BLAST of Sed0012560 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 159.5 bits (402), Expect = 4.6e-39
Identity = 97/255 (38.04%), Postives = 137/255 (53.73%), Query Frame = 0

Query: 79  STAPNSKFPKEIIVKSCPLELSSSLQHEKDLLFKFA-HCKNVVHCYGDEITTTNSGRKIY 138
           S   + +FP  + VKS     ++SL +EK +L      C  +V C+G++ T  N G +++
Sbjct: 23  SNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGEDRTVEN-GEEMH 82

Query: 139 NVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNIL 198
           N+ LEY S   L  ++      G+PE   RR+T  ++ GL H+H N  + H D+K  NIL
Sbjct: 83  NLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG-FAHCDLKLGNIL 142

Query: 199 LLPNDDDGDSFVAKIASFGLAKKLTPDVVCDHG--ICGSGPYTSPEVVSDGCLDWPADVW 258
           L      GD  V KIA FGLAK++      ++G  I G+  Y +PE V+D       DVW
Sbjct: 143 LF-----GDGAV-KIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVW 202

Query: 259 ALGCVVLEMLMGK--WSFENAYEC----------GELPQIPKEISSVGRDFIKKCLVRSP 318
           ALGCVV+EM  GK  WS +                E+P IP+E+S  GRDF+ KC V+ P
Sbjct: 203 ALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDP 262

BLAST of Sed0012560 vs. TAIR 10
Match: AT3G45670.1 (Protein kinase superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 5.6e-37
Identity = 100/286 (34.97%), Postives = 150/286 (52.45%), Query Frame = 0

Query: 55  SFWVGDCLFRKRGSEIIFLAKNKESTAPNSKFPKEIIVKSCPLELSSSLQHEKDLLFKFA 114
           S WV   L  +     ++LA +K+       +  E  +KS  +  + SL HE  +L +  
Sbjct: 95  SSWVKSRLLGEGAYGCVYLATSKDDI-----YKTERAIKSADVLKAWSLMHEGRIL-RSL 154

Query: 115 HCKNVVHCYGDEITTTNSGRKIYNVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIV 174
               V+ CYG EI    +G + YN++LEYCSG CL D MI     G+PEF+ +++  D++
Sbjct: 155 QSPFVIRCYGHEIAREGTGHQ-YNLILEYCSGQCLAD-MIEDNQGGIPEFDVKQFAIDVL 214

Query: 175 HGLYHMHCNARYIHGDIKPRNILLLPNDD--DGDSFVAKIASFGLA-KKLTPDVVCDHG- 234
            GL ++H     IH +IKP N+LL P D     + F+ KIA FGL+ +K + +     G 
Sbjct: 215 SGLSYIH-RRNIIHCEIKPDNLLLSPVDHRFRSNGFLTKIADFGLSMEKGSKEYGNGRGH 274

Query: 235 ICGSGPYTSPEVVSDGCLDWPADVWALGCVVLEMLMGK--WSFENAYECGEL-------- 294
           + G+  Y +PE++  G LD+  D+ A GC VLEML GK  W      E G+L        
Sbjct: 275 MRGTTRYMAPELIGGGLLDFAVDICAFGCSVLEMLTGKRVWG-----EYGDLAHDDWVDL 334

Query: 295 -------PQIPKEISSVGRDFIKKCLVRSPYKRRPVWLLIKHPFVC 320
                  PQI   +S+  +DF+ +CLV+ P  R  +  L+ HPF+C
Sbjct: 335 IGHSDLTPQISIRLSAEAQDFLMRCLVKEPGSRWTIGELVDHPFLC 366

BLAST of Sed0012560 vs. TAIR 10
Match: AT3G46140.1 (Protein kinase superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.2e-34
Identity = 104/318 (32.70%), Postives = 149/318 (46.86%), Query Frame = 0

Query: 22  KTKRRIVKYAKKTLPNNNWNEIFDPLSVKELHGSFWVGDCLFRKRGSEIIFLAKNKESTA 81
           K KR   K +          ++FD +  K    S W+      +     ++LA +K+   
Sbjct: 64  KRKRFTPKSSSSRSVEEITKQVFDGVVRK---SSSWIKSEFLGRGSYGSVYLATSKK--- 123

Query: 82  PNSKFPKEIIVKSCPLELSSSLQHEKDLLFKFAHCKNVVHCYGDEI----TTTNSGRKIY 141
             +K    + +KS  +  +SSL  E+ +L + +    +V CYG EI    T     R  Y
Sbjct: 124 --AKTKTTMAIKSAEISRASSLMDEERILTRLS-SPFIVRCYGHEIAREETLFGGERTNY 183

Query: 142 NVLLEYCSGSCLRDHMINYGLYGLPEFEARRYTRDIVHGLYHMHCNARYIHGDIKPRNIL 201
           N++LEYCSG  L D ++N  L GL E + +   RDI++GL  +H  A  IH DIKP NI 
Sbjct: 184 NLILEYCSGKSLFD-LVNDNLGGLSEKDVKLLARDILYGLDCIH-RANIIHCDIKPENIF 243

Query: 202 LLP--NDDDGDSFVAKIASFGLA--KKLTPDVVCDHGICGSGPYTSPEVVSDGCLDWPAD 261
           L P  N      +VAKI  FGLA  K  +          G+  Y SPE++  G +D+  D
Sbjct: 244 LTPVENRIRPSGYVAKIGDFGLALEKGSSEYEKASGHRRGTTRYMSPELIRHGIVDYAVD 303

Query: 262 VWALGCVVLEMLMGK-------------WSFENAYECGELPQIPKEISSVGRDFIKKCLV 319
            WA GC VLEML G+             W       C  +P IP  +S   + F+ +CL 
Sbjct: 304 TWAFGCTVLEMLTGQQVWGEHSDLGSVDWDILIGQSC-YIPYIPDWLSEEAQHFLSRCLK 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023516554.12.2e-11562.09mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
KAG7023508.13.1e-11461.79Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_022960664.15.4e-11461.49mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
KAG6589837.11.2e-11361.49Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper... [more]
XP_038880300.12.6e-10860.42mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SND61.2e-3938.32Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q9CAD51.2e-2335.78Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Q9ZVP51.9e-2130.20Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9FZ364.2e-2127.04Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
F4JBP31.0e-1930.17Serine/threonine-protein kinase ATG1b OS=Arabidopsis thaliana OX=3702 GN=ATG1B P... [more]
Match NameE-valueIdentityDescription
A0A6J1HBP52.6e-11461.49mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A0A0LHV19.9e-10657.86Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829... [more]
A0A5D3D3K98.4e-10558.46Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma... [more]
A0A1S3B8298.4e-10558.46mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656... [more]
A0A6J1JAH83.5e-8766.81mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT4G36950.13.1e-4339.45mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.18.4e-4138.32mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.14.6e-3938.04mitogen-activated protein kinase kinase kinase 19 [more]
AT3G45670.15.6e-3734.97Protein kinase superfamily protein [more]
AT3G46140.11.2e-3432.70Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 64..318
e-value: 2.1E-35
score: 133.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 101..318
e-value: 1.8E-32
score: 112.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 47..318
score: 28.965525
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 71..320
e-value: 6.2E-44
score: 152.5
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 161..317
e-value: 7.3E-10
score: 36.1
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 54..318
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 54..318
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 186..198
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 88..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012560.1Sed0012560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity