Sed0000442 (gene) Chayote v1

BLAST of Sed0000442 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 881.7 bits (2277), Expect = 3.1e-252
Identity = 430/516 (83.33%), Postives = 473/516 (91.67%), Query Frame = 0

Query: 16  MNSSMEETLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPFH 75
           M+S+MEETLL S+F++P L L++AL+L+FK RK +HKN PPGPPSLPVIGHLHLL+EPFH
Sbjct: 1   MHSTMEETLLFSNFEVPLLVLILALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFH 60

Query: 76  QMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYT 135
           QMLQDLSQKYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYDYT
Sbjct: 61  QMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYT 120

Query: 136 AVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIR 195
           AVGATAYGQHWRIMRRIATTELLST+RLN L++IR QEVKLWLK L+RI+G+N VRVEIR
Sbjct: 121 AVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIR 180

Query: 196 SKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 255
           SKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVLKL
Sbjct: 181 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 240

Query: 256 IDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDY 315
           +D+QGFRKRLAKVRVDGDAFAQSL+DERR K A     +D ERK +IDSLL +QQMEP+Y
Sbjct: 241 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKA-----DDNERKTMIDSLLSMQQMEPEY 300

Query: 316 YKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGD 375
           YKDDIIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE D
Sbjct: 301 YKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMD 360

Query: 376 LNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVW 435
           LN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+PKGTMLVVNAWA+HRDPKVW
Sbjct: 361 LNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVW 420

Query: 436 PDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQ 495
            DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RLD 
Sbjct: 421 EDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN 480

Query: 496 KEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           KEVDL+ GIGLTMPKA PLVAM KPR  ++HLL+QL
Sbjct: 481 KEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of Sed0000442 vs. NCBI nr
Match: XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 880.9 bits (2275), Expect = 5.3e-252
Identity = 430/516 (83.33%), Postives = 471/516 (91.28%), Query Frame = 0

Query: 16  MNSSMEETLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPFH 75
           M+S MEETLL S+F++P L L++AL+L+ K RK DHKN PPGPPSLPVIGHLHLL+EPFH
Sbjct: 1   MHSRMEETLLFSNFEVPLLVLILALLLILKFRKADHKNLPPGPPSLPVIGHLHLLKEPFH 60

Query: 76  QMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYT 135
           QMLQDLS KYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYDYT
Sbjct: 61  QMLQDLSHKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYT 120

Query: 136 AVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIR 195
           AVGATAYGQHWRIMRRIATTELLST+RLN L++IR QEVKLWLK L+RI+G+N VRVEIR
Sbjct: 121 AVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIR 180

Query: 196 SKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 255
           SKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVLKL
Sbjct: 181 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 240

Query: 256 IDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDY 315
           +D+QGFRKRLAKVRVDGDAFAQSL+DERR K A     +D ERK +IDSLL +QQMEP+Y
Sbjct: 241 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKA-----DDNERKTMIDSLLSMQQMEPEY 300

Query: 316 YKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGD 375
           YKDDIIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE D
Sbjct: 301 YKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMD 360

Query: 376 LNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVW 435
           LN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+PKGTMLVVNAWA+HRDPKVW
Sbjct: 361 LNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVW 420

Query: 436 PDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQ 495
            DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RLD 
Sbjct: 421 EDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDS 480

Query: 496 KEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           KEVDL+ GIGLTMPKASPLVAM KPR  ++HLL+QL
Sbjct: 481 KEVDLTPGIGLTMPKASPLVAMFKPRTPIIHLLSQL 511

BLAST of Sed0000442 vs. NCBI nr
Match: KAG7037779.1 (Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 878.2 bits (2268), Expect = 3.5e-251
Identity = 428/518 (82.63%), Postives = 474/518 (91.51%), Query Frame = 0

Query: 14  NIMNSSMEETLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREP 73
           NIM+S+MEETLL S+F++  L L++ ++L+FK RK ++KN PPGPPS+PVIGHLHLL+EP
Sbjct: 13  NIMHSTMEETLLFSNFEVTLLVLILGVLLIFKFRKAENKNLPPGPPSIPVIGHLHLLKEP 72

Query: 74  FHQMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYD 133
           FHQMLQDLSQKYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYD
Sbjct: 73  FHQMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYD 132

Query: 134 YTAVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVE 193
           YTAVGATAYGQHWRIMRRIATTELLST+RLN L++IR QEVKLWLK L+RI+G+N VRVE
Sbjct: 133 YTAVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVE 192

Query: 194 IRSKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVL 253
           IRSKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVL
Sbjct: 193 IRSKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVL 252

Query: 254 KLIDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEP 313
           KL+D+QGFRKRLAKVRVDGDAFAQSL+DERR K A     +D ERK +IDSLL +QQMEP
Sbjct: 253 KLVDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKA-----DDNERKTMIDSLLSMQQMEP 312

Query: 314 DYYKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEE 373
           +YYKDDIIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE
Sbjct: 313 EYYKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEE 372

Query: 374 GDLNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPK 433
            DLN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+PKGTMLVVNAWA+HRDPK
Sbjct: 373 MDLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPK 432

Query: 434 VWPDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRL 493
           VW DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RL
Sbjct: 433 VWEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERL 492

Query: 494 DQKEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           D KEVDL+ GIGLTMPKASPLVAM KPR  ++HLL+QL
Sbjct: 493 DNKEVDLTPGIGLTMPKASPLVAMFKPRAPIIHLLSQL 525

BLAST of Sed0000442 vs. NCBI nr
Match: XP_022982228.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 869.4 bits (2245), Expect = 1.6e-248
Identity = 429/517 (82.98%), Postives = 470/517 (90.91%), Query Frame = 0

Query: 16  MNSSMEETLLL-SHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPF 75
           M+S+MEETLLL S+F++  L LV+AL+++FK RK DHKN PPGPPSLPVIGHLHLL+EPF
Sbjct: 1   MHSTMEETLLLFSNFEVLLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPF 60

Query: 76  HQMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDY 135
           HQMLQDLSQKYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYDY
Sbjct: 61  HQMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDY 120

Query: 136 TAVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEI 195
           TAVGATAYGQHWRIMRRIATTELLSTHRLN L++IR QEVKLWLK L+RITG+N VRVEI
Sbjct: 121 TAVGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEI 180

Query: 196 RSKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLK 255
           RSKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVLK
Sbjct: 181 RSKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLK 240

Query: 256 LIDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPD 315
           L+D+QGFRK+LAKVRVDGDAFAQSL+DERR K A     +  ERK +IDSLL +QQMEP+
Sbjct: 241 LVDYQGFRKKLAKVRVDGDAFAQSLIDERRNKKA-----DHNERKTMIDSLLSMQQMEPE 300

Query: 316 YYKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEG 375
           YYKD IIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE 
Sbjct: 301 YYKDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEM 360

Query: 376 DLNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKV 435
           DLN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+P GTMLVVNAWA+HRDPKV
Sbjct: 361 DLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKV 420

Query: 436 WPDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLD 495
           W DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RLD
Sbjct: 421 WEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLD 480

Query: 496 QKEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
            KEVDL+ GIGLTMPK+SPLVAM KPR  + HLL+QL
Sbjct: 481 NKEVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

BLAST of Sed0000442 vs. NCBI nr
Match: XP_022138249.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 770.0 bits (1987), Expect = 1.3e-218
Identity = 380/514 (73.93%), Postives = 438/514 (85.21%), Query Frame = 0

Query: 20  MEETLLLSHFQIPFLTLVVALILL--FKLRKPDHKNRPPGPPSLPVIGHLHLLREPFHQM 79
           MEETL  S F IP L L+ ALI +  FKL+K   KN PPGPPSLPVIGHLHLLREPFHQM
Sbjct: 1   MEETLQFSRFLIP-LALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQM 60

Query: 80  LQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAV 139
           LQ+LS+KYGPI+SLSLGFR VVVVSSP+A QECF+KNDI+FA+RPR ISG+ILNY+YT+V
Sbjct: 61  LQNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSV 120

Query: 140 GATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSK 199
           GATAYGQHWRIMRRIATT+LLSTHRLN+ +++R QEVKLWLK L++ TG+N V+VEIR+K
Sbjct: 121 GATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRTK 180

Query: 200 LTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLID 259
           LTELSFNIAL +M GKRYFG  V +DSEE R+LK  MK+V LLSGASYPADFLSVLKLID
Sbjct: 181 LTELSFNIALRMMTGKRYFGVDV-EDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLID 240

Query: 260 FQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYK 319
           +QGFRKRL KV    D FAQSL+D RRE+ AS S   ++ RKN+ID++L LQ  +P YYK
Sbjct: 241 YQGFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYK 300

Query: 320 DDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLN 379
           D IIKGQ LTLLAAGTDTTAGT+EWG+SLLLNH + M+K W+EIN+ +G++RLVEE DL 
Sbjct: 301 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLT 360

Query: 380 KLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPD 439
            L +LQAIINET RLFPALPILVPRESSEDCTIGGYDIPKGTMLVVN WA+HRDPKVW D
Sbjct: 361 NLPFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQD 420

Query: 440 PTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKE 499
           PTSF+PERF+ GE VE +KLLPFGMGRRACPG GLAHR V LA+AT+IQCFEWD++   E
Sbjct: 421 PTSFQPERFENGEVVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNE 480

Query: 500 VDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           +DL QG GLTMPKA PL AMCKPR  M++LL+QL
Sbjct: 481 IDLFQGNGLTMPKAHPLEAMCKPRAPMINLLSQL 512

BLAST of Sed0000442 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 5.0e-144
Identity = 255/509 (50.10%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 23  TLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPP-SLPVIGHLHLLREPFHQMLQDL 82
           TLL +   +  L++ + L  + + R    +N PP P  +LPVIGHLHL+ +  H+ L DL
Sbjct: 5   TLLYTFLAVVLLSISLKLFPVSRRR----RNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 64

Query: 83  SQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATA 142
           S+KYG + SL LG R V+VVSSPAA +ECF+KNDI+FA+RP FI G+ + Y+YT +  + 
Sbjct: 65  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 124

Query: 143 YGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTEL 202
           YG+HWR +RR+A  E+ S   LN  + IR  EVK  L  L++ +G++  +VE++SKL+EL
Sbjct: 125 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 184

Query: 203 SFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGF 262
           SFN+ + ++AGKRYFG+ V  DS+EA+  +  + +V   +GAS P DF+  L+ IDF+ +
Sbjct: 185 SFNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNY 244

Query: 263 RKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDII 322
            K+++K+  + DAF Q L+ E R          +K    +ID LL LQ+ +P+YY D II
Sbjct: 245 EKKVSKISQEMDAFLQRLIHESR---------INKNNVTMIDHLLSLQESQPEYYTDQII 304

Query: 323 KGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHY 382
           KG  + LL AGTDT+A T+EW +SLLLNHP  +EKA TEI   +G  RL+EE DL KL Y
Sbjct: 305 KGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTY 364

Query: 383 LQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSF 442
           L  II+ET+RL PA P+LVP ESS+DC + GYD+PKGT+L+VNAWA+HRDP+ W +PT F
Sbjct: 365 LHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLF 424

Query: 443 RPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLS 502
           +PER   G  +E SKL+PFGMGRR+CPG GLA R V L +  +IQCFEW R+ + ++D++
Sbjct: 425 KPER-HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMA 484

Query: 503 QGIGLTMPKASPLVAMCKPRNSMVHLLAQ 531
           +G GLTMPKA PL A+CKPRN +  ++++
Sbjct: 485 EGSGLTMPKAQPLEALCKPRNILHKVVSE 497

BLAST of Sed0000442 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 462.2 bits (1188), Expect = 7.8e-129
Identity = 223/502 (44.42%), Postives = 337/502 (67.13%), Query Frame = 0

Query: 22  ETLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPFHQMLQDL 81
           E L L  + + +L L     ++ + RK   KN PPGPPSLP+IG+LH L+ P H+  + L
Sbjct: 2   EILSLLSYSVFYLALFFIFNIVIRARK--FKNLPPGPPSLPIIGNLHHLKRPLHRTFKGL 61

Query: 82  SQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATA 141
           S+KYG + SL  G R VVVVSS +  Q+CF+KND++ A+RPRF+SG+ + Y+YT +G+T+
Sbjct: 62  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 121

Query: 142 YGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTEL 201
           YG+HWR +RRI   ++LS HR+N+   IR  E +  +  L   +  N   +E+ S+L ++
Sbjct: 122 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 181

Query: 202 SFNIALMLMAGKRYFGEGVG-DDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQG 261
           +FN  + +++GKRY+GE     D +EA + ++ + ++  LSGA+   DF+ +L+ +DF+ 
Sbjct: 182 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 241

Query: 262 FRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKN-IIDSLLELQQMEPDYYKDD 321
             KRL  +    DAF + L++E R K         KER N +ID LL LQ  +P+YY D 
Sbjct: 242 LEKRLKDISGKTDAFLRGLIEEHRTK---------KERANTMIDHLLNLQDSQPEYYTDQ 301

Query: 322 IIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKL 381
           IIKG  L +L AGTD++A TLEW +S LLNHP  ++K   E++  +G +RLV+E DL KL
Sbjct: 302 IIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKL 361

Query: 382 HYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPT 441
            YL+ +INET RL+   P+L+P  +S++C IGGY +P+ T++++NAWA+HRDP++W + T
Sbjct: 362 TYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEAT 421

Query: 442 SFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVD 501
           +F+PERF+  +  E+ KL+ FGMGRRACPG GLA R +++ +A +IQCF+W  ++  ++D
Sbjct: 422 TFKPERFE--KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKID 481

Query: 502 LSQGIGLTMPKASPLVAMCKPR 522
           L++  G T+ K  PL AMCK R
Sbjct: 482 LAERDGFTLTKLVPLKAMCKSR 490

BLAST of Sed0000442 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 9.5e-127
Identity = 229/499 (45.89%), Postives = 323/499 (64.73%), Query Frame = 0

Query: 33  FLTLVVALILL------FKLRKPDHKNRPPGPPSLPVIGHLHLLREPFHQMLQDLSQKYG 92
           +L+L+++L  L      F       KN PPGP  LP+IG+LH L++P H     LSQKYG
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYG 64

Query: 93  PIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQHW 152
            I SL  G R VVVVSS    QECF+KNDI+ A+RP F++G+ + Y+ T V  + YG HW
Sbjct: 65  QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 124

Query: 153 RIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTELSFNIA 212
           R +RRI + E+LS+HRLN+ ++IR  E+   ++ L + +      VE+R   +E++FN  
Sbjct: 125 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTI 184

Query: 213 LMLMAGKRYFGEGVG-DDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRKRL 272
           + +++GKRY+G      D EEAR  +  +K+V  L GA+   DFL  L+  DF G  KRL
Sbjct: 185 MRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRL 244

Query: 273 AKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDIIKGQF 332
            K+    DAF Q L+DE R    +S+         +ID LL  QQ +P+YY D IIKG  
Sbjct: 245 KKISKRTDAFLQGLIDEHRFGKRNSN--------TMIDHLLTQQQSQPEYYTDQIIKGLM 304

Query: 333 LTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQAI 392
           + +L AGTDT++ T+EW +S LLNHP  M+KA  E++  IG +R V+E D++KL YLQ+I
Sbjct: 305 VVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSI 364

Query: 393 INETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRPER 452
           + ET RL  A P+LVP  SSED ++GGY+IP+ T+L+VNAW +HRDP +W DPT F+PER
Sbjct: 365 VYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPER 424

Query: 453 FKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLSQGIG 512
           F+  +  EV+KLL FG+GRRACPG  L+ R   L +  +IQCFEW R+ ++++D+ +  G
Sbjct: 425 FE--KEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKG 484

Query: 513 LTMPKASPLVAMCKPRNSM 525
           +T  K + L AMCK R+ +
Sbjct: 485 ITAGKKTSLNAMCKVRHPL 493

BLAST of Sed0000442 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 9.5e-127
Identity = 221/498 (44.38%), Postives = 329/498 (66.06%), Query Frame = 0

Query: 26  LSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPFHQMLQDLSQKY 85
           L ++ +  L+ ++ + +L K+     KN PPGPP++P+IG+LH L+ P H+    LSQ Y
Sbjct: 2   LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTY 61

Query: 86  GPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQH 145
           G I SL  G R VVVVSSP+   ECF+KNDII A+RPRF++G+ + Y+YT +G+ +YG H
Sbjct: 62  GDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDH 121

Query: 146 WRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFR---ITGENKVRVEIRSKLTELS 205
           WR +RRI T ++LS +RLN+ + +R  E    ++ L +     G    +VE+R +LTE++
Sbjct: 122 WRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMT 181

Query: 206 FNIALMLMAGKRYFG-EGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGF 265
           FN  + +++GKRY+G +G   D EEA++ +  + ++  L GA+   DFL +L+++D    
Sbjct: 182 FNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNL 241

Query: 266 RKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDII 325
            KR  ++    +AF + L++E R     S      +   +ID LL+L + +P+YY D +I
Sbjct: 242 EKRCKRIAKRSNAFLEGLIEEHRRGNIHS------DGGTMIDHLLKLSESQPEYYSDHLI 301

Query: 326 KGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHY 385
           KG    +L AGTDT+A T+EW +S LLNHP  ++KA  E++  IG  +LV+E DL+KL Y
Sbjct: 302 KGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPY 361

Query: 386 LQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSF 445
           LQ II+ET RL P  P+L+P  SSEDCTIG +++PK T+++ N W +HRDPK W D  SF
Sbjct: 362 LQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSF 421

Query: 446 RPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLS 505
           +PERF+  E  EV+K++ FG+GRRACPG  LA R V   +  +IQCFEW+R  ++++D+ 
Sbjct: 422 KPERFEKEE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMM 481

Query: 506 QGIGLTMPKASPLVAMCK 520
           +G G+TMP   PL AMCK
Sbjct: 482 EGKGITMPMKIPLRAMCK 491

BLAST of Sed0000442 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.4e-125
Identity = 238/513 (46.39%), Postives = 330/513 (64.33%), Query Frame = 0

Query: 20  MEET----LLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPS-LPVIGHLHLLREPF 79
           MEET    +L S F + F      LI+ FK  KP  +N PP PP  LP+IGHL LL+ P 
Sbjct: 1   MEETNIRVVLYSIFSLIF------LIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPI 60

Query: 80  HQMLQDLSQKY-----GPIMSLSLGFRRVVVVSS-PAAVQECFSKNDIIFASRPRFISGQ 139
           H+ L+  S+       G +MSL LG R V VVSS   A +ECF KND++ A+RP+ I G+
Sbjct: 61  HRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGK 120

Query: 140 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGEN 199
            + Y+ T + A  YG HWR +RR+ T E+ STHRLN  + +R  EV+  +  L R+ G  
Sbjct: 121 HVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTK 180

Query: 200 KVRVEIRSKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPAD 259
           K  VE++  L +L+FN  + +M GKRY+GE   D+ EEA+ ++  +  V   + +    D
Sbjct: 181 KTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDE-EEAKRVRKLVADVGANTSSGNAVD 240

Query: 260 FLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLEL 319
           ++ +L+L  F  +  R+ K+  + D F Q L+D++R +  + +         +ID LL L
Sbjct: 241 YVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETGT--------TMIDHLLVL 300

Query: 320 QQMEPDYYKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDE 379
           Q+ + +YY D IIKG  L ++ AGT+T+A TLEW LS LLNHP  + KA  EI++ +G +
Sbjct: 301 QKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLD 360

Query: 380 RLVEEGDLNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAV 439
           RL+EE DL++L YL+ I+ ET RL PA P+LVP  +SEDC IG YD+P+GT L+VNAWA+
Sbjct: 361 RLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAI 420

Query: 440 HRDPKVWPDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCF 499
           HRDP  W DP SF+PERF+  E  E  KLL FG+GRRACPG GLA R V LA+ ++IQCF
Sbjct: 421 HRDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCF 480

Query: 500 EWDRLDQKEVDLSQGIGLTMPKASPLVAMCKPR 522
           EW+R+   EVD+ +G+G T+PKA PL A+CK R
Sbjct: 481 EWERVGNVEVDMKEGVGNTVPKAIPLKAICKAR 494

BLAST of Sed0000442 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.5e-252
Identity = 430/516 (83.33%), Postives = 473/516 (91.67%), Query Frame = 0

Query: 16  MNSSMEETLLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPFH 75
           M+S+MEETLL S+F++P L L++AL+L+FK RK +HKN PPGPPSLPVIGHLHLL+EPFH
Sbjct: 1   MHSTMEETLLFSNFEVPLLVLILALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFH 60

Query: 76  QMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYT 135
           QMLQDLSQKYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYDYT
Sbjct: 61  QMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYT 120

Query: 136 AVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIR 195
           AVGATAYGQHWRIMRRIATTELLST+RLN L++IR QEVKLWLK L+RI+G+N VRVEIR
Sbjct: 121 AVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIR 180

Query: 196 SKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 255
           SKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVLKL
Sbjct: 181 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 240

Query: 256 IDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDY 315
           +D+QGFRKRLAKVRVDGDAFAQSL+DERR K A     +D ERK +IDSLL +QQMEP+Y
Sbjct: 241 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKA-----DDNERKTMIDSLLSMQQMEPEY 300

Query: 316 YKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGD 375
           YKDDIIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE D
Sbjct: 301 YKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMD 360

Query: 376 LNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVW 435
           LN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+PKGTMLVVNAWA+HRDPKVW
Sbjct: 361 LNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVW 420

Query: 436 PDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQ 495
            DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RLD 
Sbjct: 421 EDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN 480

Query: 496 KEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           KEVDL+ GIGLTMPKA PLVAM KPR  ++HLL+QL
Sbjct: 481 KEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of Sed0000442 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 7.8e-249
Identity = 429/517 (82.98%), Postives = 470/517 (90.91%), Query Frame = 0

Query: 16  MNSSMEETLLL-SHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPSLPVIGHLHLLREPF 75
           M+S+MEETLLL S+F++  L LV+AL+++FK RK DHKN PPGPPSLPVIGHLHLL+EPF
Sbjct: 1   MHSTMEETLLLFSNFEVLLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPF 60

Query: 76  HQMLQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDY 135
           HQMLQDLSQKYGPI+SLSLGFR VVVVSSPAA QECFSKNDIIFASRPR ISG+ILNYDY
Sbjct: 61  HQMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDY 120

Query: 136 TAVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEI 195
           TAVGATAYGQHWRIMRRIATTELLSTHRLN L++IR QEVKLWLK L+RITG+N VRVEI
Sbjct: 121 TAVGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEI 180

Query: 196 RSKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLK 255
           RSKLTELSFNIALMLM GKRYFG+   DDSEEARELKNTMKQVPLLSGASYPADFLSVLK
Sbjct: 181 RSKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLK 240

Query: 256 LIDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPD 315
           L+D+QGFRK+LAKVRVDGDAFAQSL+DERR K A     +  ERK +IDSLL +QQMEP+
Sbjct: 241 LVDYQGFRKKLAKVRVDGDAFAQSLIDERRNKKA-----DHNERKTMIDSLLSMQQMEPE 300

Query: 316 YYKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEG 375
           YYKD IIKGQFLTLLAAGTDTTAGTLEW LSLLLNHP+AM+KAWTEIN+ +GDERLVEE 
Sbjct: 301 YYKDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEM 360

Query: 376 DLNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKV 435
           DLN+LHYLQ IINETYRLFPALPILVPRESSEDCTIGG+D+P GTMLVVNAWA+HRDPKV
Sbjct: 361 DLNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKV 420

Query: 436 WPDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLD 495
           W DP SFRPERFK GE+VEVSKLLPFGMGRRACPG GLAHR V LA+AT+IQCFEW+RLD
Sbjct: 421 WEDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLD 480

Query: 496 QKEVDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
            KEVDL+ GIGLTMPK+SPLVAM KPR  + HLL+QL
Sbjct: 481 NKEVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

BLAST of Sed0000442 vs. ExPASy TrEMBL
Match: A0A6J1C9L7 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 6.4e-219
Identity = 380/514 (73.93%), Postives = 438/514 (85.21%), Query Frame = 0

Query: 20  MEETLLLSHFQIPFLTLVVALILL--FKLRKPDHKNRPPGPPSLPVIGHLHLLREPFHQM 79
           MEETL  S F IP L L+ ALI +  FKL+K   KN PPGPPSLPVIGHLHLLREPFHQM
Sbjct: 1   MEETLQFSRFLIP-LALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQM 60

Query: 80  LQDLSQKYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAV 139
           LQ+LS+KYGPI+SLSLGFR VVVVSSP+A QECF+KNDI+FA+RPR ISG+ILNY+YT+V
Sbjct: 61  LQNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSV 120

Query: 140 GATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSK 199
           GATAYGQHWRIMRRIATT+LLSTHRLN+ +++R QEVKLWLK L++ TG+N V+VEIR+K
Sbjct: 121 GATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRTK 180

Query: 200 LTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLID 259
           LTELSFNIAL +M GKRYFG  V +DSEE R+LK  MK+V LLSGASYPADFLSVLKLID
Sbjct: 181 LTELSFNIALRMMTGKRYFGVDV-EDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLID 240

Query: 260 FQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYK 319
           +QGFRKRL KV    D FAQSL+D RRE+ AS S   ++ RKN+ID++L LQ  +P YYK
Sbjct: 241 YQGFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYK 300

Query: 320 DDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLN 379
           D IIKGQ LTLLAAGTDTTAGT+EWG+SLLLNH + M+K W+EIN+ +G++RLVEE DL 
Sbjct: 301 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLT 360

Query: 380 KLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPD 439
            L +LQAIINET RLFPALPILVPRESSEDCTIGGYDIPKGTMLVVN WA+HRDPKVW D
Sbjct: 361 NLPFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQD 420

Query: 440 PTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKE 499
           PTSF+PERF+ GE VE +KLLPFGMGRRACPG GLAHR V LA+AT+IQCFEWD++   E
Sbjct: 421 PTSFQPERFENGEVVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNE 480

Query: 500 VDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           +DL QG GLTMPKA PL AMCKPR  M++LL+QL
Sbjct: 481 IDLFQGNGLTMPKAHPLEAMCKPRAPMINLLSQL 512

BLAST of Sed0000442 vs. ExPASy TrEMBL
Match: A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 8.4e-219
Identity = 387/509 (76.03%), Postives = 435/509 (85.46%), Query Frame = 0

Query: 31  IPFLTL-VVALILLFKLRKPDH-KNRPPGPPSLPVIGHLHLLREPFHQMLQDLSQKYGPI 90
           IP L + + +L L+ K R+  H K  PP PPSLPVIGHLHLL+EPFHQMLQD S+KYGP+
Sbjct: 20  IPLLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHLHLLKEPFHQMLQDFSKKYGPV 79

Query: 91  MSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQHWRI 150
            SL+LGFR +VVVSSP+AVQECF+KNDI+FASRPR +SG+ILNYDYTAVGATAYGQHWRI
Sbjct: 80  FSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRI 139

Query: 151 MRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKV----RVEIRSKLTELSFN 210
           MRRIATTELLST RLN L  IR +EVKLW+K L+      +V    RV +RSKLTELSFN
Sbjct: 140 MRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRAEVNKGGRVGLRSKLTELSFN 199

Query: 211 IALMLMAGKRYFGEGV-GDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRK 270
           IALMLMAG+RYFG+ +   +SEEA+ELK TMKQVPLLSGASYPADFLSVLKLIDFQGFRK
Sbjct: 200 IALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRK 259

Query: 271 RLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDIIKG 330
           RLAKVRVDGDAFAQSLVDERR+   S       ERK +IDS+L LQ+ +P+YYKDDIIKG
Sbjct: 260 RLAKVRVDGDAFAQSLVDERRKLKGSWC-----ERKTMIDSMLCLQESDPEYYKDDIIKG 319

Query: 331 QFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQ 390
           QFLTLLAAGTDTTA TLEW +SLLLNHP+ +EKAWTEIN ++GD+RL+ E D NKLHYLQ
Sbjct: 320 QFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQRLIREADTNKLHYLQ 379

Query: 391 AIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRP 450
           AIINETYRLFPALPILVPRESSEDCTIGG+DIPKGTMLVVNAWAVHRDP VW DP  F+P
Sbjct: 380 AIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRDPNVWEDPNCFKP 439

Query: 451 ERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLD-QKEVDLSQ 510
           ERFK GETVEV+KLLPFGMGRRACPG GLAHR V+LA+AT+IQCFEW +LD QK+VDL  
Sbjct: 440 ERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDNQKDVDLCP 499

Query: 511 GIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           GIGLTMPKA PL AMCKPR S+ HLL+QL
Sbjct: 500 GIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

BLAST of Sed0000442 vs. ExPASy TrEMBL
Match: A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 6.0e-217
Identity = 384/514 (74.71%), Postives = 431/514 (83.85%), Query Frame = 0

Query: 27  SHFQIPFLTL-VVALILLFKLRKPDHKN--RPPGPPSLPVIGHLHLLREPFHQMLQDLSQ 86
           SHF  P L + +  L L+ K R+ +H     PP PPSLPVIGHLHLL+EPFHQMLQDLS 
Sbjct: 14  SHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSL 73

Query: 87  KYGPIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYG 146
           KYGP+ SL+LGFR +VVVSSP+AV ECF+KNDI+FASRPR +SG+ILNYDYTAVGATAYG
Sbjct: 74  KYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYG 133

Query: 147 QHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKV----RVEIRSKLT 206
           QHWRIMRRIATTELLST RLN    +R +EVKLW+K L+      +V    RV +RSKLT
Sbjct: 134 QHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLT 193

Query: 207 ELSFNIALMLMAGKRYFGEGVGD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDF 266
           ELSFNIALML AG+RYFGE V + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKLIDF
Sbjct: 194 ELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDF 253

Query: 267 QGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKD 326
           QGFRKRLAKVRVDGDAFAQSLVDERRE   S       ERK +ID++L LQ+ +P+YYKD
Sbjct: 254 QGFRKRLAKVRVDGDAFAQSLVDERRELKGSWC-----ERKTMIDAMLSLQESDPEYYKD 313

Query: 327 DIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNK 386
           DIIKGQFLTLLAAGTDT A TLEW +SLLLNHP  M KAWTEIN ++GD+RLV E D N 
Sbjct: 314 DIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNN 373

Query: 387 LHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDP 446
           LHYLQ+IINETYRLFPALPILVPRESSEDCTIGG+DIPKGTMLVVNAWAVHR+P+VW DP
Sbjct: 374 LHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDP 433

Query: 447 TSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLD-QKE 506
             F+PERFK GETVEVSKLLPFGMGRRACPG GLAHR V+LA+AT+IQCFEW +LD +K+
Sbjct: 434 NCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDNKKD 493

Query: 507 VDLSQGIGLTMPKASPLVAMCKPRNSMVHLLAQL 532
           VDL  GIGLTMPKA PL AMCKPR S+ HLL+QL
Sbjct: 494 VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of Sed0000442 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 462.6 bits (1189), Expect = 4.2e-130
Identity = 240/500 (48.00%), Postives = 335/500 (67.00%), Query Frame = 0

Query: 33  FLTLVVALILLFKLRKPDHK-NRPPGPP-SLPVIGHLHLLREPFHQMLQDLSQKY--GPI 92
           F  L V L L++ + K   K N PP P  SLPVIGHL LL+ P H+    LSQ     PI
Sbjct: 8   FSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPI 67

Query: 93  MSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQHWRI 152
            SL LG R V V SS +  +ECF+KND++ A+RP FI  + + YDYT + A +YG HWR 
Sbjct: 68  FSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRN 127

Query: 153 MRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTELSFNIALM 212
           +RRI + E+ S HRLN+ + IR  E++  +  L R   +  V+V+++S L++L+FN  L 
Sbjct: 128 LRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILR 187

Query: 213 LMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKV 272
           ++AGKRY+G+GV DD  EA+ ++  +  V   +GA    D+L VL+L+    +  R+ K+
Sbjct: 188 MVAGKRYYGDGVEDD-PEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKL 247

Query: 273 RVDGDAFAQSLVDERREKMASSSNENDKERKN-IIDSLLELQQMEPDYYKDDIIKGQFLT 332
               D F Q LVDE+RE          KE+ N +ID LL LQ+ +PDY+ D IIKG  L 
Sbjct: 248 AGRLDEFLQGLVDEKREA---------KEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLA 307

Query: 333 LLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQAIIN 392
           L+ AGTDT+A TLEW LS +LNHP  + KA  EI+  IG +RL++E D++ L YLQ I++
Sbjct: 308 LILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVS 367

Query: 393 ETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRPERFK 452
           ET RL+PA P+L+P  +SEDC + GYD+P+GT+L+ N WA+HRDP++W DP SF+PERF+
Sbjct: 368 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFE 427

Query: 453 AGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLSQGIGLT 512
             +  E  KL+PFG+GRRACPG GLAHR + L + ++IQC EW+++ + EVD+S+G G+T
Sbjct: 428 --KEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE-EVDMSEGKGVT 487

Query: 513 MPKASPLVAMCKPRNSMVHL 528
           MPKA PL AMC+ R S+V +
Sbjct: 488 MPKAKPLEAMCRARPSVVKI 492

BLAST of Sed0000442 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 234/502 (46.61%), Postives = 333/502 (66.33%), Query Frame = 0

Query: 31  IPFLTLVVALILLFKLRKPDHKNRPPGPP-SLPVIGHLHLLREPFHQMLQDLSQKYG--P 90
           + FL+L    I L   R     N PP P  SLPVIGHLHLL+ P H+    LS+  G  P
Sbjct: 9   LSFLSLFTLAIFLL-TRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 68

Query: 91  IMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQHWR 150
           +  L LG R V V+SS +  +ECF+KND++ A+RP+F   + L Y+ T + + +YG HWR
Sbjct: 69  VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 128

Query: 151 IMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTELSFNIAL 210
            +RRIA  E+ STHRLN+ + IR  E++  +  L R +    V VE+++ LT L+ N  +
Sbjct: 129 NLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTI 188

Query: 211 MLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAK 270
            ++AGKRYFGE    D+++A+ +KN + +    +GA  P D+LS+L+ +    + KR+  
Sbjct: 189 RMLAGKRYFGE----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKN 248

Query: 271 VRVDGDAFAQSLVDERREKMASSSNENDKER-KNIIDSLLELQQMEPDYYKDDIIKGQFL 330
           +    D F Q LVDE+R          +KE+ + +ID LL LQ ++PDYY D IIKG  L
Sbjct: 249 LGNRFDTFLQKLVDEKRA---------EKEKGETMIDHLLALQDIQPDYYTDVIIKGIIL 308

Query: 331 TLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQAII 390
           TL+ AGTDT++ TLEW +S LLNHP  ++KA  EI++ +G +RLV+E D+  L YLQ+I+
Sbjct: 309 TLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIV 368

Query: 391 NETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRPERF 450
            ET R++PA+P+L+P  SSEDC +GGYDIP GTM++ NAWA+HRDP+VW DP  F+PERF
Sbjct: 369 LETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF 428

Query: 451 KAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLSQGIGL 510
           +  +  E  KL+ FGMGRRACPG GLAHR +  A+ +++QCFEW+R+ +  VD+++  G 
Sbjct: 429 E--KEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGA 488

Query: 511 TMPKASPLVAMCKPRNSMVHLL 529
           T+PKA PL AMCK R+ +  L+
Sbjct: 489 TLPKAIPLRAMCKARSIVDKLI 492

BLAST of Sed0000442 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 451.4 bits (1160), Expect = 9.8e-127
Identity = 231/500 (46.20%), Postives = 333/500 (66.60%), Query Frame = 0

Query: 33  FLTLVVALILLFKLRKPDHK-NRPPGPP-SLPVIGHLHLLREPFHQMLQDLSQKYG--PI 92
           F    + L L+F + +   K N PP P  +LPVIGHL LL+ P H++   +SQ  G  PI
Sbjct: 8   FTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPI 67

Query: 93  MSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQHWRI 152
           +SL LG R + VVSS +  +ECF+KND+I A+R   IS + ++Y  + V + +Y +HWR 
Sbjct: 68  ISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRN 127

Query: 153 MRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTELSFNIALM 212
           +RRI   E+ S HRLN+   IR  E++  +  L R +     +VE++S  ++L+FN  + 
Sbjct: 128 LRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIR 187

Query: 213 LMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKV 272
           ++AGK Y+G+G  DD  EA+ ++  + +    SG    AD++ +L  I +     R+ K+
Sbjct: 188 MLAGKCYYGDGKEDD-PEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKL 247

Query: 273 RVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDIIKGQFLTL 332
               D F Q LVDE+RE         +K+   ++D LL LQ+ +P+YY D IIKG  L+L
Sbjct: 248 AGRLDEFLQGLVDEKRE-------GKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSL 307

Query: 333 LAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQAIINE 392
           +A GTDTTA TLEW LS LLN+P  + KA  EI+ +IG +RL+EE D+  L YLQ I++E
Sbjct: 308 IAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSE 367

Query: 393 TYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRPERFKA 452
           T RL+PA P+L+P  +S+DC +GGYD+P+GTML+ NAWA+HRDP +W DPTSF+PERF+ 
Sbjct: 368 TLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFE- 427

Query: 453 GETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLSQGIGLTM 512
            +  E  KL+PFG+GRRACPG GLA R VTL++ ++IQCFEW+R+ ++EVD+++G GLTM
Sbjct: 428 -KEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTM 487

Query: 513 PKASPLVAMCKPRNSMVHLL 529
           PKA PL AMC+ R+ +  +L
Sbjct: 488 PKARPLEAMCRARDFVGKIL 495

BLAST of Sed0000442 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 451.4 bits (1160), Expect = 9.8e-127
Identity = 238/513 (46.39%), Postives = 330/513 (64.33%), Query Frame = 0

Query: 20  MEET----LLLSHFQIPFLTLVVALILLFKLRKPDHKNRPPGPPS-LPVIGHLHLLREPF 79
           MEET    +L S F + F      LI+ FK  KP  +N PP PP  LP+IGHL LL+ P 
Sbjct: 1   MEETNIRVVLYSIFSLIF------LIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPI 60

Query: 80  HQMLQDLSQKY-----GPIMSLSLGFRRVVVVSS-PAAVQECFSKNDIIFASRPRFISGQ 139
           H+ L+  S+       G +MSL LG R V VVSS   A +ECF KND++ A+RP+ I G+
Sbjct: 61  HRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGK 120

Query: 140 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGEN 199
            + Y+ T + A  YG HWR +RR+ T E+ STHRLN  + +R  EV+  +  L R+ G  
Sbjct: 121 HVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTK 180

Query: 200 KVRVEIRSKLTELSFNIALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPAD 259
           K  VE++  L +L+FN  + +M GKRY+GE   D+ EEA+ ++  +  V   + +    D
Sbjct: 181 KTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDE-EEAKRVRKLVADVGANTSSGNAVD 240

Query: 260 FLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLEL 319
           ++ +L+L  F  +  R+ K+  + D F Q L+D++R +  + +         +ID LL L
Sbjct: 241 YVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETGT--------TMIDHLLVL 300

Query: 320 QQMEPDYYKDDIIKGQFLTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDE 379
           Q+ + +YY D IIKG  L ++ AGT+T+A TLEW LS LLNHP  + KA  EI++ +G +
Sbjct: 301 QKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLD 360

Query: 380 RLVEEGDLNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAV 439
           RL+EE DL++L YL+ I+ ET RL PA P+LVP  +SEDC IG YD+P+GT L+VNAWA+
Sbjct: 361 RLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAI 420

Query: 440 HRDPKVWPDPTSFRPERFKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCF 499
           HRDP  W DP SF+PERF+  E  E  KLL FG+GRRACPG GLA R V LA+ ++IQCF
Sbjct: 421 HRDPNTWDDPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCF 480

Query: 500 EWDRLDQKEVDLSQGIGLTMPKASPLVAMCKPR 522
           EW+R+   EVD+ +G+G T+PKA PL A+CK R
Sbjct: 481 EWERVGNVEVDMKEGVGNTVPKAIPLKAICKAR 494

BLAST of Sed0000442 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-124
Identity = 234/497 (47.08%), Postives = 327/497 (65.79%), Query Frame = 0

Query: 29  FQIPFLTLVVALILLFKLRKPDHKNRPPGPP-SLPVIGHLHLLREPFHQMLQDLSQKYG- 88
           F   F++L +  I+    R+P   N PP P  +LPVIGHL LL+ P H++   +S+  G 
Sbjct: 8   FTFLFISLSLTFIIGRIKRRP---NLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGD 67

Query: 89  -PIMSLSLGFRRVVVVSSPAAVQECFSKNDIIFASRPRFISGQILNYDYTAVGATAYGQH 148
            PI+SL LG R V VVSS +  +ECF+KND++ A+R   ++ + ++Y  T V   +YG H
Sbjct: 68  APIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDH 127

Query: 149 WRIMRRIATTELLSTHRLNTLVDIRHQEVKLWLKGLFRITGENKVRVEIRSKLTELSFNI 208
           WR +RRI   E+ S HRLN+   IR  E+   +  L R +     +VE++S  + L+FN 
Sbjct: 128 WRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNN 187

Query: 209 ALMLMAGKRYFGEGVGDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLIDFQGFRKRL 268
            + ++AGK Y+G+G  DD  EA+ ++  + +     GA   AD+L +L  I   G  KR+
Sbjct: 188 IIRMLAGKCYYGDGAEDD-PEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRI 247

Query: 269 AKVRVDGDAFAQSLVDERREKMASSSNENDKERKNIIDSLLELQQMEPDYYKDDIIKGQF 328
            K+    D F Q LVDERRE         +K +  ++D LL LQ+ +P+YY D+IIKG  
Sbjct: 248 KKIASRLDEFLQGLVDERRE-------GKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 307

Query: 329 LTLLAAGTDTTAGTLEWGLSLLLNHPSAMEKAWTEINDIIGDERLVEEGDLNKLHYLQAI 388
           L+L+ AGTDT+A TLEW LS LLNHP  + KA  EI++ +G  RLVEE DL+ L YLQ I
Sbjct: 308 LSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNI 367

Query: 389 INETYRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNAWAVHRDPKVWPDPTSFRPER 448
           ++E+ RL+PA P+LVP  +SEDC +GGY +P+GTML+ NAWA+HRDPK+W DPTSF+PER
Sbjct: 368 VSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPER 427

Query: 449 FKAGETVEVSKLLPFGMGRRACPGGGLAHREVTLAMATMIQCFEWDRLDQKEVDLSQ-GI 508
           F+  +  E  KLL FG+GRRACPG GLA R  +L + ++IQCFEW+R+ ++EVD+++ G 
Sbjct: 428 FE--KEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGG 487

Query: 509 GLTMPKASPLVAMCKPR 522
           G+ MPKA PLVAMCK R
Sbjct: 488 GVIMPKAIPLVAMCKAR 489