PI0028314 (gene) Melon (PI 482460) v1

Overview


Name	PI0028314
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Sarcosine oxidase
Location	chr04: 30731623 .. 30733216 (-)
RNA-Seq Expression	PI0028314
Synteny	PI0028314

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTTCTTATCGGAGACTCCGTCGGAGAGGGAAAAGAAAAACGAGCACCAAATCCAACCCCAGAGAGAAATGGCTGCTTCCAATACTCAATTTGATGTAATCGTCGTCGGTGCCGGCGTAATGGGCAGTTCGACGGCGTACCATCTCGCTAAAACAGGGAACAGAGTTCTGATTCTCGAGCAATTTGATTTTCTTCATCACAGAGGCTCTTCTCATGGTGAATCTCGTACCATACGCGCCACCTATCCAGAGGATTATTACCATGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGGCGGAGGCGGAAATTGGCTACAGAGTCTATTTTCCGGCGGAACAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCTGCCGTCGTTGAGACCTGCCGAAAGCATTCGATCCCTCATCTGGTTCTGGATCGTGGGGAACTGGCGGAGAAGTACTCAGGTAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGTAAATACGGCGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTTTGGCTTACAAAAACGGCGCCATTTTGAAGGATAATGCGAAAGTAGTGGAGATTAAGAGAGATGAGAGTAATGGAAGAATAGTTGTTTCAACAGCCAATGGGGAGAGTTTTCGTGGGAAAAAATGTGTTGTGACAGTTGGAGCTTGGAGTAAAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGGCAATTCAGCCATTGGAGGCTACTGTTTCCTATTGGAGAATCAAGGAAGGGTACGAGGCCGAGTACGCGATCGGAGGGGACTTTCCAACGTTTGCTAGTTATGGCGACCCGTATGTTTATGGGACACCGTCATTGGAGTTTCCAGGACTGATTAAGGTGGCTATACATGGTGGACATCAATGCAATCCGGACAAGCGGTCGTGGGGGAATGGAGGGCGGCTACCGATAGGTGCTTTAAAGGAGTGGATAGAGGGGAAGTTTGGAGGGAGGGTGGATTCAAGTGGACCGGTGTCGACGCAATTGTGTATGTACTCAATGACTCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTGGTGATTGGTGGTGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGATCGGGAGGATACTAGCAGAGCTTGCATTGAAGGGGGCAGCGGAGGGGGTGGATCTGAAGTATTTCAAGATAGCAAGGTTTGAAAAGAATCTAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACCAGAATGATTAATCTCAAAGCCATCCATGGTTTACTTTGTTTAATTCTTCTTTCTCTCATGATTCAGAACATTGAATAACTCCTCAACTCTTTCTTTTTAAATTTCAATACGTGGGAGTAAGAGATTCAAACCTTACCTAACCACTTTGTCAAGAAAAATGTCTTAGCTATGTGTCATTTTCTTATTTAGAGAATGTTAATTAGGTGGTAAGCATTCAATTGAGTTTATGAAGTATAGAATTAATAAATTGGAATAAGAAAGTCAGTGTTTGAAGTGTTTGAAGTGTTGAATTATGG

mRNA sequence

GTTTTTCTTATCGGAGACTCCGTCGGAGAGGGAAAAGAAAAACGAGCACCAAATCCAACCCCAGAGAGAAATGGCTGCTTCCAATACTCAATTTGATGTAATCGTCGTCGGTGCCGGCGTAATGGGCAGTTCGACGGCGTACCATCTCGCTAAAACAGGGAACAGAGTTCTGATTCTCGAGCAATTTGATTTTCTTCATCACAGAGGCTCTTCTCATGGTGAATCTCGTACCATACGCGCCACCTATCCAGAGGATTATTACCATGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGGCGGAGGCGGAAATTGGCTACAGAGTCTATTTTCCGGCGGAACAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCTGCCGTCGTTGAGACCTGCCGAAAGCATTCGATCCCTCATCTGGTTCTGGATCGTGGGGAACTGGCGGAGAAGTACTCAGGTAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGTAAATACGGCGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTTTGGCTTACAAAAACGGCGCCATTTTGAAGGATAATGCGAAAGTAGTGGAGATTAAGAGAGATGAGAGTAATGGAAGAATAGTTGTTTCAACAGCCAATGGGGAGAGTTTTCGTGGGAAAAAATGTGTTGTGACAGTTGGAGCTTGGAGTAAAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGGCAATTCAGCCATTGGAGGCTACTGTTTCCTATTGGAGAATCAAGGAAGGGTACGAGGCCGAGTACGCGATCGGAGGGGACTTTCCAACGTTTGCTAGTTATGGCGACCCGTATGTTTATGGGACACCGTCATTGGAGTTTCCAGGACTGATTAAGGTGGCTATACATGGTGGACATCAATGCAATCCGGACAAGCGGTCGTGGGGGAATGGAGGGCGGCTACCGATAGGTGCTTTAAAGGAGTGGATAGAGGGGAAGTTTGGAGGGAGGGTGGATTCAAGTGGACCGGTGTCGACGCAATTGTGTATGTACTCAATGACTCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTGGTGATTGGTGGTGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGATCGGGAGGATACTAGCAGAGCTTGCATTGAAGGGGGCAGCGGAGGGGGTGGATCTGAAGTATTTCAAGATAGCAAGGTTTGAAAAGAATCTAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACCAGAATGATTAATCTCAAAGCCATCCATGGTTTACTTTGTTTAATTCTTCTTTCTCTCATGATTCAGAACATTGAATAACTCCTCAACTCTTTCTTTTTAAATTTCAATACGTGGGAGTAAGAGATTCAAACCTTACCTAACCACTTTGTCAAGAAAAATGTCTTAGCTATGTGTCATTTTCTTATTTAGAGAATGTTAATTAGGTGGTAAGCATTCAATTGAGTTTATGAAGTATAGAATTAATAAATTGGAATAAGAAAGTCAGTGTTTGAAGTGTTTGAAGTGTTGAATTATGG

Coding sequence (CDS)

ATGGCTGCTTCCAATACTCAATTTGATGTAATCGTCGTCGGTGCCGGCGTAATGGGCAGTTCGACGGCGTACCATCTCGCTAAAACAGGGAACAGAGTTCTGATTCTCGAGCAATTTGATTTTCTTCATCACAGAGGCTCTTCTCATGGTGAATCTCGTACCATACGCGCCACCTATCCAGAGGATTATTACCATGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGGCGGAGGCGGAAATTGGCTACAGAGTCTATTTTCCGGCGGAACAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCTGCCGTCGTTGAGACCTGCCGAAAGCATTCGATCCCTCATCTGGTTCTGGATCGTGGGGAACTGGCGGAGAAGTACTCAGGTAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGTAAATACGGCGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTTTGGCTTACAAAAACGGCGCCATTTTGAAGGATAATGCGAAAGTAGTGGAGATTAAGAGAGATGAGAGTAATGGAAGAATAGTTGTTTCAACAGCCAATGGGGAGAGTTTTCGTGGGAAAAAATGTGTTGTGACAGTTGGAGCTTGGAGTAAAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGGCAATTCAGCCATTGGAGGCTACTGTTTCCTATTGGAGAATCAAGGAAGGGTACGAGGCCGAGTACGCGATCGGAGGGGACTTTCCAACGTTTGCTAGTTATGGCGACCCGTATGTTTATGGGACACCGTCATTGGAGTTTCCAGGACTGATTAAGGTGGCTATACATGGTGGACATCAATGCAATCCGGACAAGCGGTCGTGGGGGAATGGAGGGCGGCTACCGATAGGTGCTTTAAAGGAGTGGATAGAGGGGAAGTTTGGAGGGAGGGTGGATTCAAGTGGACCGGTGTCGACGCAATTGTGTATGTACTCAATGACTCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTGGTGATTGGTGGTGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGATCGGGAGGATACTAGCAGAGCTTGCATTGAAGGGGGCAGCGGAGGGGGTGGATCTGAAGTATTTCAAGATAGCAAGGTTTGAAAAGAATCTAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACCAGAATGATTAA

Protein sequence

MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIKRDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEGYEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIGALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLHQND

Homology

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BLAST of PI0028314 vs. ExPASy Swiss-Prot
Match: Q9SJA7 (Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 4.3e-159
Identity = 264/405 (65.19%), Postives = 324/405 (80.00%), Query Frame = 0


Query: 7   QFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHG 66
           +FDVIVVGAGVMGSS AY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYPEDYY+ 
Sbjct: 8   RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYYYS 67

Query: 67  LVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRGELA 126
           +V ES  LW  A++EIGY+V+FP +Q D+GP+D +SL +VV TC+KH + H V+D   ++
Sbjct: 68  MVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMDSHAVS 127

Query: 127 EKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIKRD-ESN 186
           E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQTLA  +GAIL+DN KV  IKRD ES 
Sbjct: 128 EHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKRDGESG 187

Query: 187 GRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEGYEAEY 246
             ++V T  G+ F GKKC+VT GAW  KLVK+V GI+  ++PLE TV YWRIKEG+E ++
Sbjct: 188 EGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEGHEEKF 247

Query: 247 AIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIGALKEW 306
            I G+FPTFASYG PYVYGTPSLE+PGLIKVA+HGG+ C+PDKR WG G +L    LKEW
Sbjct: 248 TIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKLE--ELKEW 307

Query: 307 IEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMSPAI 366
           I+ +FGG VDS GPV+TQLCMYSMTPDEDFVIDFLGGEF +DVV+GGGFSGHGFKM+PA+
Sbjct: 308 IKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAV 367

Query: 367 GRILAELAL--KGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKL 409
           GRILA++A+  +    GV++K F + RFE N KGN K + DQV L
Sbjct: 368 GRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

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BLAST of PI0028314 vs. ExPASy Swiss-Prot
Match: Q9P0Z9 (Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 1.7e-62
Identity = 143/396 (36.11%), Postives = 213/396 (53.79%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAA    +D IV+GAG+ G  TAYHLAK   R+L+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTAYHLAKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L  +     +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
              EL +++   + +P   V +    GGVI   KA+   Q    + G I++D  KVVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
              + G +V       S++ K  V+T G W+ +L++ + GIE+ +Q L   V YWR  E 
Sbjct: 181 ---NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--EM 240

Query: 241 YEAEYAIGGDFPTFASYG--DPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLP 300
               Y +   FP F   G    ++YG P+ E+PGL+KV+ H G+  +P++R      R  
Sbjct: 241 VPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPT-ARTD 300

Query: 301 IG---ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFS 360
           IG    L  ++            P   + CMY+ TPDE F++D        ++VIG GFS
Sbjct: 301 IGDVQILSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFS 360

Query: 361 GHGFKMSPAIGRILAELALKGAAEGVDLKYFKIARF 392
           GHGFK++P +G+IL EL++K      DL  F+I+RF
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRF 382

[Close]

BLAST of PI0028314 vs. ExPASy Swiss-Prot
Match: Q29RU9 (Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 4.9e-62
Identity = 143/397 (36.02%), Postives = 208/397 (52.39%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAA    +D IV+GAG+ G  TAYHLAK   +VL+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQRELYDAIVIGAGIQGCFTAYHLAKHSKKVLLLEQFFLPHSRGSSHGQSRIIRRAYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y LW   E E G ++Y     L +G  ++  L  +  T  +  + H  L
Sbjct: 61  EDFYTQMMAECYSLWAQLEHEAGTQLYRQTGLLLLGMKENPELKIIQATLSRQGVEHQCL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
              EL +++   + +    V +    GGV+   KA+   Q    + G I+ D  KVVEIK
Sbjct: 121 SSEELKQRFP-NIRLARGEVGLLEVSGGVLYADKALRALQDAIRQLGGIVHDGEKVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
               +G  V+      S++ K  ++T G W+ +L++ +G  EL +Q L   V YW  +E 
Sbjct: 181 ----SGLPVMVKTTSRSYQAKSLIITAGPWTNRLLRPLGA-ELPLQTLRINVCYW--QEK 240

Query: 241 YEAEYAIGGDFPTFASYG----DPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSW--GNG 300
               Y++   FP F   G      ++YG PS E+PGL+KV  H G+  +P++R       
Sbjct: 241 VPGSYSVSQAFPCFMGLGLSLAPHHIYGLPSREYPGLMKVCYHHGNNADPEERDCPAAFS 300

Query: 301 GRLPIGALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGF 360
               +  L  ++            P   + CMY+ TPD  FV+D        ++VIG GF
Sbjct: 301 DIQDVHILSGFVRDHLPDL--QPEPAVMEHCMYTNTPDGHFVLD--RHPKYDNIVIGAGF 360

Query: 361 SGHGFKMSPAIGRILAELALKGAAEGVDLKYFKIARF 392
           SGHGFK+SP +G+IL EL++K      DL  F+I+RF
Sbjct: 361 SGHGFKLSPVVGKILYELSMK-LTPSYDLTPFRISRF 384

[Close]

BLAST of PI0028314 vs. ExPASy Swiss-Prot
Match: P79371 (Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.4e-61
Identity = 141/396 (35.61%), Postives = 211/396 (53.28%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAA    +D IV+GAG+ G  T YHL K   R+L+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTVYHLVKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L  +     +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
              EL +++   + +P   V +    GGVI   KA+   Q    + G I++D  KVVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
              + G +V       S++ K  V+T G W+ +L++ + GIE+ +Q L   V YWR  E 
Sbjct: 181 ---NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--EM 240

Query: 241 YEAEYAIGGDFPTFASYG--DPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLP 300
               Y +   FP F   G    ++YG P+ E+PGL+KV+ H G+  +P++R      R  
Sbjct: 241 VPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPT-ARTD 300

Query: 301 IG---ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFS 360
           IG    L  ++            P   + CMY+ TPDE F++D        ++VIG GFS
Sbjct: 301 IGDVQILSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFS 360

Query: 361 GHGFKMSPAIGRILAELALKGAAEGVDLKYFKIARF 392
           GHGFK++P +G+IL EL++K      DL  F+I+RF
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRF 382

[Close]

BLAST of PI0028314 vs. ExPASy Swiss-Prot
Match: Q9D826 (Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-58
Identity = 139/403 (34.49%), Postives = 211/403 (52.36%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAA    +D IV+GAG+ G  TAYHLAK    VL+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQTDFWDAIVIGAGIQGCFTAYHLAKHSKSVLLLEQFFLPHSRGSSHGQSRIIRKAYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y+ W   E E G +++   E L +G  ++  L  +  T  +  I H  L
Sbjct: 61  EDFYTMMMKECYQTWAQLEREAGTQLHRQTELLLLGTKENPGLKTIQATLSRQGIDHEYL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
              +L +++   +      V +  K GGV+   KA+   Q +  + G  + D  KVVEI+
Sbjct: 121 SSVDLKQRFP-NIRFTRGEVGLLDKTGGVLYADKALRALQHIICQLGGTVCDGEKVVEIR 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
                G  V      +S++    V+T G W+ +L+  + GIEL +Q L   V YWR  E 
Sbjct: 181 ----PGLPVTVKTTLKSYQANSLVITAGPWTNRLLHPL-GIELPLQTLRINVCYWR--EK 240

Query: 241 YEAEYAIGGDFPTF--ASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGN--GGR 300
               Y +   FP          ++YG P+ E+PGL+K+  H G   +P++R         
Sbjct: 241 VPGSYGVSQAFPCILGLDLAPHHIYGLPASEYPGLMKICYHHGDNVDPEERDCPKTFSDI 300

Query: 301 LPIGALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSG 360
             +  L  ++     G    + P   + CMY+ TPDE F++D    +++ ++VIG GFSG
Sbjct: 301 QDVQILCHFVRDHLPGL--RAEPDIMERCMYTNTPDEHFILD-CHPKYD-NIVIGAGFSG 360

Query: 361 HGFKMSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNV 400
           HGFK++P +G+IL EL++K      DL  F+++RF    K ++
Sbjct: 361 HGFKLAPVVGKILYELSMK-LPPSYDLAPFRMSRFSTLSKAHL 390

[Close]

BLAST of PI0028314 vs. ExPASy TrEMBL
Match: A0A0A0LJG6 (Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.6e-217
Identity = 370/409 (90.46%), Postives = 387/409 (94.62%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+T FDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY+GLVMESYELWRMAE EIGY+VYFP EQLDIG  DDKSL AVV+TCRKHSIPHLVL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           D GEL EKYSGRVEIPADWV VWSKYGGVIKPTKAVSM+QTLAYKNGA++KDNA+VVEIK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVS ANGESFRGKKCVVTVGAWSKKLVKSVGGIEL I+PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAIGG FPT ASYG+PYVYGTPSLEFPGLIKVAIHGGH+CNPDKRSWG GGRLPI 
Sbjct: 241 FEAEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSSGPVSTQ CMYSMTPD DFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLH 410
           MSP IGRILAELAL GAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLH
Sbjct: 361 MSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of PI0028314 vs. ExPASy TrEMBL
Match: A0A5D3E5F8 (Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006680 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.8e-216
Identity = 370/412 (89.81%), Postives = 390/412 (94.66%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+TQFDVIVVGAGVMGSSTAYHLAKTGNRVL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWRMAEAEIG++VY+PAEQLDIGPS+ +SL AVV+TC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DRGEL EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLAYKNG +LKDNA+VVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVSTANGESF GKKCVVTVGAWSKKLVKSVGGIEL I PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAI G FPT ASYG+PYVYGTPSLEFPGLIKVAIH G+ CNPDKRSWG  GRLPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGRDGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLHQND 413
           MSPAIGRILAELALKGAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLHQND
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLHQND 412

[Close]

BLAST of PI0028314 vs. ExPASy TrEMBL
Match: A0A1S3CR44 (Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 8.8e-216
Identity = 370/412 (89.81%), Postives = 390/412 (94.66%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+TQFDVIVVGAGVMGSSTAYHLAKTGNRVL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWRMAEAEIG++VY+PAEQLDIGPS+ +SL AVV+TC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DRGEL EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLAYKNG +LKDNA+VVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVSTANGESF GKKCVVTVGAWSKKLVKSVGGIEL I PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAI G FPT ASYG+PYVYGTPSLEFPGLIKVAIH G+ CNPDKRSWG  GRLPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGREGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLHQND 413
           MSPAIGRILAELALKGAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLHQND
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLHQND 412

[Close]

BLAST of PI0028314 vs. ExPASy TrEMBL
Match: A0A6J1FC48 (Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.4e-213
Identity = 363/409 (88.75%), Postives = 387/409 (94.62%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S TQ+DVIVVGAGVMGSSTAYHLAKTGN+VL LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWR AEAEIGYRVYFPAEQLDIG SDDKSLAA V+TC+KHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DR +LAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLA+KNGA LKDNA+VVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           R+ S+G +VVSTANGE FRGKKCVVTVGAW++KLVKSVGGIEL IQPLEATVSYWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
            E EYAIGGDFPTFASYGD Y+YGTPSLEFPGLIKVA+HGGH+C+PDKRSWG+GG LPI 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWIEG+FGGRVDSS PVSTQLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLH 410
           MSPAIGRILA+LALKG AEG++LKYF+IARFE+N KGNVKSFADQV+LH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 409

[Close]

BLAST of PI0028314 vs. ExPASy TrEMBL
Match: A0A6J1IIQ6 (Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.2e-212
Identity = 361/409 (88.26%), Postives = 387/409 (94.62%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S TQ+DVIVVGAGVMGSSTAYHLAKTGN+VLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWR AEAEIGYRVYFPAEQLDIG SDDKSLAA V+TC+KHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DR +LAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLA+KNGA LKDNA+VVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RD S+G +VVS ANGE FRGKKCVVTVGAW++KLVKSVGGIEL IQPLEATVSYWRIKEG
Sbjct: 181 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
            E EYAIGG+FPTFASYGD Y+YGTPSLEFPGLIKVA+HGGH+C+PDKRSWG+GG+LPI 
Sbjct: 241 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALK+WIEG+FGGRVDSS PVSTQLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLH 410
           MSPAIGRILA+LALKG AEG++LKYF+IARFE+N KGNVK FADQV+LH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 409

[Close]

BLAST of PI0028314 vs. NCBI nr
Match: XP_038896765.1 (probable sarcosine oxidase [Benincasa hispida])

HSP 1 Score: 776.9 bits (2005), Expect = 8.4e-221
Identity = 376/408 (92.16%), Postives = 393/408 (96.32%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S TQFDVIVVGAGVMGSST YHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQFDVIVVGAGVMGSSTGYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVV+TCRKHSIPH+VL
Sbjct: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVDTCRKHSIPHMVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DRG+LAEKYSGRVEIP+DWVAVWSKYGGVIKPTKAVSMFQ LAYKNGA+LKDNA+VVEIK
Sbjct: 121 DRGQLAEKYSGRVEIPSDWVAVWSKYGGVIKPTKAVSMFQCLAYKNGAVLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVS ANGESF GKKCVVTVGAW++KLVKSV  IEL IQPLEATVSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFSGKKCVVTVGAWARKLVKSVSRIELPIQPLEATVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
            EAEYAIGGDFPTFASYG+PYVYGTPSLEFPGLIKVA+HGGHQCNPDKRSWG+GGRLPI 
Sbjct: 241 AEAEYAIGGDFPTFASYGNPYVYGTPSLEFPGLIKVAMHGGHQCNPDKRSWGSGGRLPIS 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWIE +FGGRVDSS P++TQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEERFGGRVDSSDPIATQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKL 409
           MSPAIGRILAELALKG AEGV+LKYFKIARFE+N KGN+KSFADQVKL
Sbjct: 361 MSPAIGRILAELALKGEAEGVELKYFKIARFEENPKGNIKSFADQVKL 408

[Close]

BLAST of PI0028314 vs. NCBI nr
Match: XP_004136447.1 (probable sarcosine oxidase [Cucumis sativus] >KGN60176.1 hypothetical protein Csa_000993 [Cucumis sativus])

HSP 1 Score: 765.0 bits (1974), Expect = 3.3e-217
Identity = 370/409 (90.46%), Postives = 387/409 (94.62%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+T FDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY+GLVMESYELWRMAE EIGY+VYFP EQLDIG  DDKSL AVV+TCRKHSIPHLVL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           D GEL EKYSGRVEIPADWV VWSKYGGVIKPTKAVSM+QTLAYKNGA++KDNA+VVEIK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVS ANGESFRGKKCVVTVGAWSKKLVKSVGGIEL I+PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAIGG FPT ASYG+PYVYGTPSLEFPGLIKVAIHGGH+CNPDKRSWG GGRLPI 
Sbjct: 241 FEAEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSSGPVSTQ CMYSMTPD DFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLH 410
           MSP IGRILAELAL GAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLH
Sbjct: 361 MSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of PI0028314 vs. NCBI nr
Match: KAA0038750.1 (putative sarcosine oxidase [Cucumis melo var. makuwa] >TYK31363.1 putative sarcosine oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 759.6 bits (1960), Expect = 1.4e-215
Identity = 370/412 (89.81%), Postives = 390/412 (94.66%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+TQFDVIVVGAGVMGSSTAYHLAKTGNRVL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWRMAEAEIG++VY+PAEQLDIGPS+ +SL AVV+TC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DRGEL EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLAYKNG +LKDNA+VVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVSTANGESF GKKCVVTVGAWSKKLVKSVGGIEL I PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAI G FPT ASYG+PYVYGTPSLEFPGLIKVAIH G+ CNPDKRSWG  GRLPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGRDGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLHQND 413
           MSPAIGRILAELALKGAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLHQND
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLHQND 412

[Close]

BLAST of PI0028314 vs. NCBI nr
Match: XP_008466296.1 (PREDICTED: probable sarcosine oxidase [Cucumis melo])

HSP 1 Score: 759.2 bits (1959), Expect = 1.8e-215
Identity = 370/412 (89.81%), Postives = 390/412 (94.66%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MAAS+TQFDVIVVGAGVMGSSTAYHLAKTGNRVL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWRMAEAEIG++VY+PAEQLDIGPS+ +SL AVV+TC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DRGEL EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLAYKNG +LKDNA+VVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           RDESNGRIVVSTANGESF GKKCVVTVGAWSKKLVKSVGGIEL I PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
           +EAEYAI G FPT ASYG+PYVYGTPSLEFPGLIKVAIH G+ CNPDKRSWG  GRLPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGREGRLPIA 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWI+ KFGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLHQND 413
           MSPAIGRILAELALKGAAEGV+LKYFK+ARFE+N KGNVKSFADQVKLHQND
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLHQND 412

[Close]

BLAST of PI0028314 vs. NCBI nr
Match: XP_022936028.1 (probable sarcosine oxidase [Cucurbita moschata])

HSP 1 Score: 751.9 bits (1940), Expect = 2.9e-213
Identity = 363/409 (88.75%), Postives = 387/409 (94.62%), Query Frame = 0


Query: 1   MAASNTQFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S TQ+DVIVVGAGVMGSSTAYHLAKTGN+VL LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWR AEAEIGYRVYFPAEQLDIG SDDKSLAA V+TC+KHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DRGELAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIK 180
           DR +LAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLA+KNGA LKDNA+VVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDESNGRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEG 240
           R+ S+G +VVSTANGE FRGKKCVVTVGAW++KLVKSVGGIEL IQPLEATVSYWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 YEAEYAIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIG 300
            E EYAIGGDFPTFASYGD Y+YGTPSLEFPGLIKVA+HGGH+C+PDKRSWG+GG LPI 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 301 ALKEWIEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360
           ALKEWIEG+FGGRVDSS PVSTQLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRILAELALKGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKLH 410
           MSPAIGRILA+LALKG AEG++LKYF+IARFE+N KGNVKSFADQV+LH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 409

[Close]

BLAST of PI0028314 vs. TAIR 10
Match: AT2G24580.1 (FAD-dependent oxidoreductase family protein )

HSP 1 Score: 562.4 bits (1448), Expect = 3.0e-160
Identity = 264/405 (65.19%), Postives = 324/405 (80.00%), Query Frame = 0


Query: 7   QFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHG 66
           +FDVIVVGAGVMGSS AY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYPEDYY+ 
Sbjct: 8   RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYYYS 67

Query: 67  LVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRGELA 126
           +V ES  LW  A++EIGY+V+FP +Q D+GP+D +SL +VV TC+KH + H V+D   ++
Sbjct: 68  MVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMDSHAVS 127

Query: 127 EKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAYKNGAILKDNAKVVEIKRD-ESN 186
           E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQTLA  +GAIL+DN KV  IKRD ES 
Sbjct: 128 EHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKRDGESG 187

Query: 187 GRIVVSTANGESFRGKKCVVTVGAWSKKLVKSVGGIELAIQPLEATVSYWRIKEGYEAEY 246
             ++V T  G+ F GKKC+VT GAW  KLVK+V GI+  ++PLE TV YWRIKEG+E ++
Sbjct: 188 EGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEGHEEKF 247

Query: 247 AIGGDFPTFASYGDPYVYGTPSLEFPGLIKVAIHGGHQCNPDKRSWGNGGRLPIGALKEW 306
            I G+FPTFASYG PYVYGTPSLE+PGLIKVA+HGG+ C+PDKR WG G +L    LKEW
Sbjct: 248 TIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKLE--ELKEW 307

Query: 307 IEGKFGGRVDSSGPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMSPAI 366
           I+ +FGG VDS GPV+TQLCMYSMTPDEDFVIDFLGGEF +DVV+GGGFSGHGFKM+PA+
Sbjct: 308 IKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAV 367

Query: 367 GRILAELAL--KGAAEGVDLKYFKIARFEKNLKGNVKSFADQVKL 409
           GRILA++A+  +    GV++K F + RFE N KGN K + DQV L
Sbjct: 368 GRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

The following BLAST results are available for this feature:

BLAST of PI0028314 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJA7	4.3e-159	65.19	Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=...	[more]
Q9P0Z9	1.7e-62	36.11	Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2	[more]
Q29RU9	4.9e-62	36.02	Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2	[more]
P79371	1.4e-61	35.61	Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=...	[more]
Q9D826	1.5e-58	34.49	Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1	[more]

BLAST of PI0028314 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJG6	1.6e-217	90.46	Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1	[more]
A0A5D3E5F8	6.8e-216	89.81	Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CR44	8.8e-216	89.81	Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1	[more]
A0A6J1FC48	1.4e-213	88.75	Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1	[more]
A0A6J1IIQ6	1.2e-212	88.26	Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1	[more]

BLAST of PI0028314 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896765.1	8.4e-221	92.16	probable sarcosine oxidase [Benincasa hispida]	[more]
XP_004136447.1	3.3e-217	90.46	probable sarcosine oxidase [Cucumis sativus] >KGN60176.1 hypothetical protein Cs...	[more]
KAA0038750.1	1.4e-215	89.81	putative sarcosine oxidase [Cucumis melo var. makuwa] >TYK31363.1 putative sarco...	[more]
XP_008466296.1	1.8e-215	89.81	PREDICTED: probable sarcosine oxidase [Cucumis melo]	[more]
XP_022936028.1	2.9e-213	88.75	probable sarcosine oxidase [Cucurbita moschata]	[more]

BLAST of PI0028314 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24580.1	3.0e-160	65.19	FAD-dependent oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 9..377 e-value: 8.7E-101 score: 339.7
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 7..393
IPR006076	FAD dependent oxidoreductase	PFAM	PF01266	DAO	coord: 9..372 e-value: 6.5E-45 score: 154.2
IPR006281	Sarcosine oxidase, monomeric	TIGRFAM	TIGR01377	TIGR01377	coord: 8..396 e-value: 1.9E-150 score: 498.8
None	No IPR available	GENE3D	3.30.9.10		coord: 63..331 e-value: 8.7E-101 score: 339.7
None	No IPR available	PANTHER	PTHR10961:SF7	PEROXISOMAL SARCOSINE OXIDASE	coord: 4..401
None	No IPR available	PANTHER	PTHR10961	PEROXISOMAL SARCOSINE OXIDASE	coord: 4..401
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 226..331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028314.1	PI0028314.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046653	tetrahydrofolate metabolic process
molecular_function	GO:0008115	sarcosine oxidase activity
molecular_function	GO:0016491	oxidoreductase activity