PI0021589 (gene) Melon (PI 482460) v1

Overview
NamePI0021589
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAuxin-responsive GH3 family protein
Locationchr08: 19978141 .. 19980573 (+)
RNA-Seq ExpressionPI0021589
SyntenyPI0021589
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTCTCTTCATATCCTTCAAACTTGTTTTAATTTTATAAATAATGGCCGTTTTTGATAATTCTGAATTTTCATCTCCTCTTGGACCGCCTGCTTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACAAATGCTGACTCAGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTGAACCGGTTCCGGCTCAATGGAGCTACGGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTTACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCCAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGGTTTAGTTTCGTAATTCCCCCATTTTTTTTGTTATTTATTATAAATTAATTAAACTAAATTAAATTAGGGTTAATTATATACGTTTAATAATTTATGCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATTTGTAAGTTTTTTTTTTTTTTAAATTTAATTTGGTGAAAATTATTTAAAAACAAAAAACAAAAACAAAAACAAAAAATTGACAATGAAAAAAAATATATATAATTTTCAGGTATGTGCCGGGGTTGGACAAAGGAAAAGGACTTTACTTTTTATTCGTGAAGGCAGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGCTATTACAAGAGCGATATCTTCAAGACTCGGCCTTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTCTATGCGCTGATTCATTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTCGGTGCGGTTTTCGCCTCCGGTCTTCTCCGTGCCATTCGCTTTCTCCAGATCAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAATCGGGAGCTAGCGGAATTCATCGCAAATGAATGCTCCACGGAGGAATGGGAAGGGATTATCACGAAAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTTCCGCTCTTTCACGTGACTCCCCGCCTCGACTTGTTGACCTTGCTGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGTGTCGATACCGGGTTGGTGATGTCCTTGAAGTCACTGGGTTCCATAACGCCGCCCCTCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAGGCGGTCGAGAACGCGTCATCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGCTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATATTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCAGATAACTCAATTGGACCATTGGAGATTAGAGTGGTGAAAAACGGGACGTTTGAAGAGTTAATGGACTACGCGATCTCGAGAGGCGCATCAATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACGCCCATTGTTGAGCTTCTCGACTCTAGGGTAACTTCGGTTCACTTTAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGAGGTCGACCAACAAAAATAAAAATTTCACATTAAAAAAAAATTGAAAGAGACCCATATGTGTAAAAAGATTATTATTATGATGATGATGTAGAAGCCAAAGTAGAAGAGGATAGAGTTTGAGTTTGTTGGATTATGCATATGGCTTTTAGAAGTGTCTTTTGATTATTATTATTATTATTGTAGAGTTTTGTGTTTTTTTTTATTTTTGATTTCTTAGGGAAAATTAATTTCTAAGTTCATGTGTCATGTCTGTGCAATAAGGTTCAAGTAATTAATGTCTAAAATATGGGCTTCATGATGTAATTGTTCTTTTACAAAATATGTCTGTTGGTCTATTATATATTGAATCTTTTTGTC

mRNA sequence

CTTCTCTCTTCATATCCTTCAAACTTGTTTTAATTTTATAAATAATGGCCGTTTTTGATAATTCTGAATTTTCATCTCCTCTTGGACCGCCTGCTTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACAAATGCTGACTCAGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTGAACCGGTTCCGGCTCAATGGAGCTACGGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTTACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCCAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATTTGTATGTGCCGGGGTTGGACAAAGGAAAAGGACTTTACTTTTTATTCGTGAAGGCAGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGCTATTACAAGAGCGATATCTTCAAGACTCGGCCTTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTCTATGCGCTGATTCATTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTCGGTGCGGTTTTCGCCTCCGGTCTTCTCCGTGCCATTCGCTTTCTCCAGATCAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAATCGGGAGCTAGCGGAATTCATCGCAAATGAATGCTCCACGGAGGAATGGGAAGGGATTATCACGAAAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTTCCGCTCTTTCACGTGACTCCCCGCCTCGACTTGTTGACCTTGCTGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGTGTCGATACCGGGTTGGTGATGTCCTTGAAGTCACTGGGTTCCATAACGCCGCCCCTCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAGGCGGTCGAGAACGCGTCATCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGCTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATATTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCAGATAACTCAATTGGACCATTGGAGATTAGAGTGGTGAAAAACGGGACGTTTGAAGAGTTAATGGACTACGCGATCTCGAGAGGCGCATCAATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACGCCCATTGTTGAGCTTCTCGACTCTAGGGTAACTTCGGTTCACTTTAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGAGGTCGACCAACAAAAATAAAAATTTCACATTAAAAAAAAATTGAAAGAGACCCATATGTGTAAAAAGATTATTATTATGATGATGATGTAGAAGCCAAAGTAGAAGAGGATAGAGTTTGAGTTTGTTGGATTATGCATATGGCTTTTAGAAGTGTCTTTTGATTATTATTATTATTATTGTAGAGTTTTGTGTTTTTTTTTATTTTTGATTTCTTAGGGAAAATTAATTTCTAAGTTCATGTGTCATGTCTGTGCAATAAGGTTCAAGTAATTAATGTCTAAAATATGGGCTTCATGATGTAATTGTTCTTTTACAAAATATGTCTGTTGGTCTATTATATATTGAATCTTTTTGTC

Coding sequence (CDS)

ATGGCCGTTTTTGATAATTCTGAATTTTCATCTCCTCTTGGACCGCCTGCTTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACAAATGCTGACTCAGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTGAACCGGTTCCGGCTCAATGGAGCTACGGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTTACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCCAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATTTGTATGTGCCGGGGTTGGACAAAGGAAAAGGACTTTACTTTTTATTCGTGAAGGCAGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGCTATTACAAGAGCGATATCTTCAAGACTCGGCCTTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTCTATGCGCTGATTCATTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTCGGTGCGGTTTTCGCCTCCGGTCTTCTCCGTGCCATTCGCTTTCTCCAGATCAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAATCGGGAGCTAGCGGAATTCATCGCAAATGAATGCTCCACGGAGGAATGGGAAGGGATTATCACGAAAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTTCCGCTCTTTCACGTGACTCCCCGCCTCGACTTGTTGACCTTGCTGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGTGTCGATACCGGGTTGGTGATGTCCTTGAAGTCACTGGGTTCCATAACGCCGCCCCTCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAGGCGGTCGAGAACGCGTCATCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGCTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATATTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCAGATAACTCAATTGGACCATTGGAGATTAGAGTGGTGAAAAACGGGACGTTTGAAGAGTTAATGGACTACGCGATCTCGAGAGGCGCATCAATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACGCCCATTGTTGAGCTTCTCGACTCTAGGGTAACTTCGGTTCACTTTAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGA

Protein sequence

MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERRR
Homology
BLAST of PI0021589 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 2.7e-274
Identity = 463/603 (76.78%), Postives = 519/603 (86.07%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S  SSPLGPPACEKDAKALRFIEE T NAD+VQ+ +LAEIL +NA TEY+ RF L GAT
Sbjct: 4   DSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDTFK+K PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 64  DRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           E++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+ F+
Sbjct: 124 ELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFR 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
           +RPYDP+N YTSPNEA+LC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAIRFLQ+
Sbjct: 184 SRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIW 305
           +W + AHDI  G L+ +ITD  +R+C++   +  P+  LAEFI  EC ++ WE IIT+IW
Sbjct: 244 HWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 363

Query: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425
           PNM YFEF+P   +            + V+L DV +GKEYELV+TTYAGLCRYRVGD+L 
Sbjct: 364 PNMAYFEFIPLGGT------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILR 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLL-REFNTTVVEYTSYAD 485
           VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKAVENASS+L  E  + V EYTSYAD
Sbjct: 424 VTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYAD 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           T TIPGHYV+YWELLV+D     SH   E L +CCL +EESLNSVYRQ RVADNS+GPLE
Sbjct: 484 TSTIPGHYVLYWELLVRDGARQPSH---ETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 605
           IRVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPE
Sbjct: 544 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 589

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 589

BLAST of PI0021589 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 1.2e-261
Identity = 451/603 (74.79%), Postives = 510/603 (84.58%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S   SP+      KD KALRFIEE T N D VQ++V+ EIL++N+ TEY+ RF L G T
Sbjct: 4   DSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DR TFK+K PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E
Sbjct: 64  DRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           +M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK
Sbjct: 124 DMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFK 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
            RP DP+N YTSPNEA+LC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ 
Sbjct: 184 RRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTE-EWEGIITKI 305
           NWK+LA DISTGTL+ +I+D  ++E + SK LT P++ELA+FI + C  +  WEGIITKI
Sbjct: 244 NWKELADDISTGTLSSRISDPAIKESM-SKILTKPDQELADFITSVCGQDNSWEGIITKI 303

Query: 306 WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTI 365
           WPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTI
Sbjct: 304 WPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTI 363

Query: 366 MPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVL 425
           MPNM YFEFLPH       +        LV+LADVEVGKEYELVITTYAGL RYRVGD+L
Sbjct: 364 MPNMAYFEFLPHHEVPTEKS-------ELVELADVEVGKEYELVITTYAGLNRYRVGDIL 423

Query: 426 EVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYAD 485
           +VTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+
Sbjct: 424 QVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAE 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           TKTIPGHYVIYWELLVKD     + P DE++ +CCL +EESLNSVYRQ RVAD SIGPLE
Sbjct: 484 TKTIPGHYVIYWELLVKD---QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 605
           IRVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE
Sbjct: 544 IRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPE 595

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 595

BLAST of PI0021589 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 880.6 bits (2274), Expect = 1.0e-254
Identity = 446/612 (72.88%), Postives = 504/612 (82.35%), Query Frame = 0

Query: 1   MAVF-DNSEFSSPLGPPAC----EKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEY 60
           MAV  D S   + L  PA     E D + LRFI+E TTN D+VQ+RVL EIL +NA TEY
Sbjct: 1   MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEY 60

Query: 61  VNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAG 120
           + +  L+GATDR  F++K PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAG
Sbjct: 61  LTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAG 120

Query: 121 ERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVL 180
           ERKLMPTI +E++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVL
Sbjct: 121 ERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVL 180

Query: 181 TSYYKSDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASG 240
           TSYYKSD FK RPYDP+++YTSP  A+LCAD+FQSMY QM+CGL  R+ VLR+GAVFASG
Sbjct: 181 TSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASG 240

Query: 241 LLRAIRFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTE 300
           LLRAIRFLQ+NW+QLA DI +G L P++TD  +RE + +  L +P  ELA+ I  ECS  
Sbjct: 241 LLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILLPDP--ELAKLIRAECSKG 300

Query: 301 EWEGIITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMC 360
           +W GIIT++WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL PMC
Sbjct: 301 DWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMC 360

Query: 361 KPSDVTYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGL 420
            PS+V+YTIMPNMGYFEFLP D +  AS        +LVDLA VEVG+EYELVITTYAGL
Sbjct: 361 DPSEVSYTIMPNMGYFEFLPVDETGAASG----DATQLVDLARVEVGREYELVITTYAGL 420

Query: 421 CRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTT 480
            RYRVGDVL VTGFHNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+AVE AS+LLR    +
Sbjct: 421 NRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGAS 480

Query: 481 VVEYTSYADTKTIPGHYVIYWELLVKD-NETMNSHPTDEILNQCCLAIEESLNSVYRQGR 540
           VVEYTS A TK IPGHYVIYWELL K    T+    T   L +CCL +EE+LN+VYRQ R
Sbjct: 481 VVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADT---LGRCCLEMEEALNTVYRQSR 540

Query: 541 VADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHF 600
           VAD SIGPLEIRVV+ GTFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HF
Sbjct: 541 VADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHF 600

Query: 601 SPSKPHWTPERR 607
           SP+ PHWTP RR
Sbjct: 601 SPALPHWTPARR 603

BLAST of PI0021589 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 1.0e-254
Identity = 446/612 (72.88%), Postives = 504/612 (82.35%), Query Frame = 0

Query: 1   MAVF-DNSEFSSPLGPPAC----EKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEY 60
           MAV  D S   + L  PA     E D + LRFI+E TTN D+VQ+RVL EIL +NA TEY
Sbjct: 1   MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEY 60

Query: 61  VNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAG 120
           + +  L+GATDR  F++K PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAG
Sbjct: 61  LTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAG 120

Query: 121 ERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVL 180
           ERKLMPTI +E++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVL
Sbjct: 121 ERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVL 180

Query: 181 TSYYKSDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASG 240
           TSYYKSD FK RPYDP+++YTSP  A+LCAD+FQSMY QM+CGL  R+ VLR+GAVFASG
Sbjct: 181 TSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASG 240

Query: 241 LLRAIRFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTE 300
           LLRAIRFLQ+NW+QLA DI +G L P++TD  +RE + +  L +P  ELA+ I  ECS  
Sbjct: 241 LLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILLPDP--ELAKLIRAECSKG 300

Query: 301 EWEGIITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMC 360
           +W GIIT++WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL PMC
Sbjct: 301 DWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMC 360

Query: 361 KPSDVTYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGL 420
            PS+V+YTIMPNMGYFEFLP D +  AS        +LVDLA VEVG+EYELVITTYAGL
Sbjct: 361 DPSEVSYTIMPNMGYFEFLPVDETGAASG----DATQLVDLARVEVGREYELVITTYAGL 420

Query: 421 CRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTT 480
            RYRVGDVL VTGFHNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+AVE AS+LLR    +
Sbjct: 421 NRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGAS 480

Query: 481 VVEYTSYADTKTIPGHYVIYWELLVKD-NETMNSHPTDEILNQCCLAIEESLNSVYRQGR 540
           VVEYTS A TK IPGHYVIYWELL K    T+    T   L +CCL +EE+LN+VYRQ R
Sbjct: 481 VVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADT---LGRCCLEMEEALNTVYRQSR 540

Query: 541 VADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHF 600
           VAD SIGPLEIRVV+ GTFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HF
Sbjct: 541 VADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHF 600

Query: 601 SPSKPHWTPERR 607
           SP+ PHWTP RR
Sbjct: 601 SPALPHWTPARR 603

BLAST of PI0021589 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 1.1e-251
Identity = 422/594 (71.04%), Postives = 503/594 (84.68%), Query Frame = 0

Query: 17  ACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPV 76
           ACE+DA+ L FIEE T   D+VQ+RVLA IL +N   EY+ R  + G TDR+ FK++ PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 77  VTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSL 136
           VTYEDL+P+I+RIANGDRS I SSHPI+EFLTSSGTSAGERKLMPTI++E++RRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 137 LMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYT 196
           LMP+MNLYVPGLDKGKGLYFLF+K+ETKTPGGL ARPVLTSYYKSD FK RP+DP+N YT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 197 SPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQINWKQLAHDIST 256
           SP  A+LC D+FQSMY QMLCGL+ R +VLRVGAVFASGLLRAIRFLQ++W++LAHDI T
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 257 GTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWPNTKYLDVIVT 316
           GTL+ K+T+  +R+ + ++ L  P+ ELA F+  EC  ++WEGIIT++WPNTKYLDVIVT
Sbjct: 265 GTLSAKVTEPSIRDAV-AEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVT 324

Query: 317 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPH 376
           GAMAQYIPTL+FYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNMGYFE +PH
Sbjct: 325 GAMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPH 384

Query: 377 DSSSRASALSRDS-PPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQ 436
           D    A  L RD+ PPRLVDLAD EVG+EYELVITTYAGLCRYRVGD+L+VTGFHNAAPQ
Sbjct: 385 DPD--APPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQ 444

Query: 437 FRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGHYVIY 496
           FRFVRRKNVLLSIDSDKTDEAELQ AVE AS+LL  +  ++VEYTS AD  TIPGHYV+Y
Sbjct: 445 FRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVY 504

Query: 497 WELLVKDNETMNSHPTDE---ILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGT 556
           WEL+V++         +E   +  +CCL +EE+LN+VYRQGR  + +IGPLEIRVV+ GT
Sbjct: 505 WELMVREGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGT 564

Query: 557 FEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 607
           FEE+MDYAISRGASINQYKAPRCV+F PI+ELL+SRV S HFSP+ P ++P ++
Sbjct: 565 FEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

BLAST of PI0021589 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 598/607 (98.52%), Postives = 603/607 (99.34%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DNSEFSSPLGPPACEKDAKALRFIEETTTNAD VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPN ELAEFI+NEC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. ExPASy TrEMBL
Match: A0A1S3B0G2 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484816 PE=3 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 598/607 (98.52%), Postives = 603/607 (99.34%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DNSEFSSPLGPPACEKDAKALRFIEETTTNAD VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPN ELAEFI+NEC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. ExPASy TrEMBL
Match: A0A0A0KJM5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1)

HSP 1 Score: 1205.3 bits (3117), Expect = 0.0e+00
Identity = 593/607 (97.69%), Postives = 598/607 (98.52%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DNSEFS+PLGPPACEKDAKALRFIEETTTN DSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNP  ELAEFI++EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDSSSRA ALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. ExPASy TrEMBL
Match: A0A6J1GH54 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111453877 PE=3 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 557/602 (92.52%), Postives = 575/602 (95.51%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S+ SSPL PP  +KDAKALRFIEE TTN D+VQQRVLAEIL++NA+TEYVNRFRLNGAT
Sbjct: 4   DSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLNGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+E
Sbjct: 64  DRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
           TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIRFLQ+
Sbjct: 184 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIW 305
           NWKQLAHDISTGTLNPKITD CLRECI SKYLTN N E+AEFI+ ECSTEEWEGIIT+IW
Sbjct: 244 NWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363

Query: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425
           PNMGYFEFLPHD+SS    LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRVGDVLE
Sbjct: 364 PNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLE 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADT 485
           VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYTSYADT
Sbjct: 424 VTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYADT 483

Query: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545
           KTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEI
Sbjct: 484 KTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 543

Query: 546 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 605
           RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPER
Sbjct: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPER 603

Query: 606 RR 608
           RR
Sbjct: 604 RR 603

BLAST of PI0021589 vs. ExPASy TrEMBL
Match: A0A6J1IRM5 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111478730 PE=3 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 556/601 (92.51%), Postives = 575/601 (95.67%), Query Frame = 0

Query: 7   SEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATD 66
           S+ SSPL PP  ++DAKALRFIEE TTNAD+VQQRVLAEIL++NA+TEYVNRFRLNGATD
Sbjct: 5   SQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFRLNGATD 64

Query: 67  RDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEE 126
           RDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+EE
Sbjct: 65  RDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQEE 124

Query: 127 MERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKT 186
           MERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKT
Sbjct: 125 MERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKT 184

Query: 187 RPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQIN 246
           RPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIRFLQ+N
Sbjct: 185 RPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQLN 244

Query: 247 WKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWP 306
           WKQLAHDISTGTLNPKITD CLRECI SKYLTNPN E+AEFI+ ECSTEEWEGIIT+IWP
Sbjct: 245 WKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIITRIWP 304

Query: 307 NTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMP 366
           NTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIMP
Sbjct: 305 NTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMP 364

Query: 367 NMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEV 426
           NMGYFEFLPH +SS    LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRVGDVLEV
Sbjct: 365 NMGYFEFLPHHTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLEV 424

Query: 427 TGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTK 486
           TGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYTSYADTK
Sbjct: 425 TGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYADTK 484

Query: 487 TIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIR 546
           TIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEIR
Sbjct: 485 TIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIR 544

Query: 547 VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606
           VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSP+KPHWTPERR
Sbjct: 545 VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPHWTPERR 603

Query: 607 R 608
           R
Sbjct: 605 R 603

BLAST of PI0021589 vs. NCBI nr
Match: XP_008440335.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >TYK12848.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 598/607 (98.52%), Postives = 603/607 (99.34%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DNSEFSSPLGPPACEKDAKALRFIEETTTNAD VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPN ELAEFI+NEC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. NCBI nr
Match: XP_004141893.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48572.1 hypothetical protein Csa_003850 [Cucumis sativus])

HSP 1 Score: 1205.3 bits (3117), Expect = 0.0e+00
Identity = 593/607 (97.69%), Postives = 598/607 (98.52%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DNSEFS+PLGPPACEKDAKALRFIEETTTN DSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNP  ELAEFI++EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDSSSRA ALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. NCBI nr
Match: XP_038881175.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 579/607 (95.39%), Postives = 595/607 (98.02%), Query Frame = 0

Query: 1   MAVFDNSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV DN +FSSPLGPPACEKDAKALRFI+ETT NADSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNPQFSSPLGPPACEKDAKALRFIQETTANADSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRPYDP+NDYTSPNEAVLCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPYNDYTSPNEAVLCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAI 240

Query: 241 RFLQINWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGI 300
           RFLQ+NWKQLAHDI+TGTLNPKITD+CLRECIT+KYLTNPN ELAEFI+ ECSTEEWEGI
Sbjct: 241 RFLQLNWKQLAHDIATGTLNPKITDSCLRECITTKYLTNPNPELAEFISKECSTEEWEGI 300

Query: 301 ITKIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           IT+IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKP +V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPDEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420
           TYTIMPNMGYFEFLPHDS S+A ALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSLSQAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVL+VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+E+ASSLLREFNTTVVEYT
Sbjct: 421 GDVLKVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIESASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNE MN HPTDEILNQCCLAIEE+LNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNEAMNCHPTDEILNQCCLAIEENLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSP+KPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPAKPH 600

Query: 601 WTPERRR 608
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of PI0021589 vs. NCBI nr
Match: KAG6604151.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 558/602 (92.69%), Postives = 576/602 (95.68%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S+ SSPL PP  +KDAKALRFIEE TTN D+VQQRVLAEIL++NA+TEYVNRFRLNGAT
Sbjct: 4   DSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLNGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+E
Sbjct: 64  DRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
           TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIRFLQ+
Sbjct: 184 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIW 305
           NWKQLAHDISTGTLNPKITD CLRECI SKYLTNPN E+AEFI+ ECSTEEWEGIIT+IW
Sbjct: 244 NWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363

Query: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425
           PNMGYFEFLPHD+SS    LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRVGDVLE
Sbjct: 364 PNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLE 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADT 485
           VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYTSYADT
Sbjct: 424 VTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYADT 483

Query: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545
           KTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEI
Sbjct: 484 KTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 543

Query: 546 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 605
           RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPER
Sbjct: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPER 603

Query: 606 RR 608
           RR
Sbjct: 604 RR 603

BLAST of PI0021589 vs. NCBI nr
Match: XP_023543141.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 558/602 (92.69%), Postives = 576/602 (95.68%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S+ SSPL PP  +KDAKALRFI+E TTN D+VQQRVLAEIL++NA+TEYVNRFRLNGAT
Sbjct: 4   DSQLSSPLPPPPSDKDAKALRFIQEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLNGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+E
Sbjct: 64  DRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
           TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIRFLQ+
Sbjct: 184 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIW 305
           NWKQLAHDISTGTLNPKITD CLRECI SKYLTNPN E+AEFI+ ECSTEEWEGIIT+IW
Sbjct: 244 NWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363

Query: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425
           PNMGYFEFLPHD+SS    LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRVGDVLE
Sbjct: 364 PNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLE 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADT 485
           VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRE NT+VVEYTSYADT
Sbjct: 424 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRELNTSVVEYTSYADT 483

Query: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545
           KTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEI
Sbjct: 484 KTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 543

Query: 546 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 605
           RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPER
Sbjct: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPER 603

Query: 606 RR 608
           RR
Sbjct: 604 RR 603

BLAST of PI0021589 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 945.7 bits (2443), Expect = 1.9e-275
Identity = 463/603 (76.78%), Postives = 519/603 (86.07%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S  SSPLGPPACEKDAKALRFIEE T NAD+VQ+ +LAEIL +NA TEY+ RF L GAT
Sbjct: 4   DSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDTFK+K PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 64  DRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           E++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+ F+
Sbjct: 124 ELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFR 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
           +RPYDP+N YTSPNEA+LC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAIRFLQ+
Sbjct: 184 SRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIW 305
           +W + AHDI  G L+ +ITD  +R+C++   +  P+  LAEFI  EC ++ WE IIT+IW
Sbjct: 244 HWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 363

Query: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425
           PNM YFEF+P   +            + V+L DV +GKEYELV+TTYAGLCRYRVGD+L 
Sbjct: 364 PNMAYFEFIPLGGT------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILR 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLL-REFNTTVVEYTSYAD 485
           VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKAVENASS+L  E  + V EYTSYAD
Sbjct: 424 VTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYAD 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           T TIPGHYV+YWELLV+D     SH   E L +CCL +EESLNSVYRQ RVADNS+GPLE
Sbjct: 484 TSTIPGHYVLYWELLVRDGARQPSH---ETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 605
           IRVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPE
Sbjct: 544 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 589

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 589

BLAST of PI0021589 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 922.2 bits (2382), Expect = 2.2e-268
Identity = 451/589 (76.57%), Postives = 513/589 (87.10%), Query Frame = 0

Query: 19  EKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVT 78
           EKD KAL+FIEE T N DSVQ++VL EILT+N++TEY+ RF L+G  DR TFKSK PVVT
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 79  YEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLM 138
           YEDL+P+IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMPTI+E+++RRQLLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 139 PIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSP 198
           P+MNLYVPGLDKGKGLYFLFVK+E+KT GGL ARPVLTSYYKSD FK RPYDP+N YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 199 NEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQINWKQLAHDISTGT 258
           NEA+LC+DS QSMY QMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA DISTGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 259 LNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWPNTKYLDVIVTGA 318
           L+ +I D  ++  + SK LT P++ELAEF+   CS E WEGIITKIWPNTKYLDVIVTGA
Sbjct: 258 LSSRIFDPAIKNRM-SKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGA 317

Query: 319 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDS 378
           MAQYIPTLE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM YFEFLPH+ 
Sbjct: 318 MAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNH 377

Query: 379 SSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRF 438
               +A +      LV+LA+VEVGKEYELVITTYAGL RYRVGD+L VTGFHN+APQF+F
Sbjct: 378 DGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKF 437

Query: 439 VRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGHYVIYWEL 498
           +RRKNVLLS++SDKTDEAELQKAVENAS L  E  T V+EYTSYA+TKTIPGHYVIYWEL
Sbjct: 438 IRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWEL 497

Query: 499 LVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMD 558
           L +D    N+  ++E++ +CCL +EESLNSVYRQ RVAD SIGPLEIRVV+NGTFEELMD
Sbjct: 498 LGRDQS--NALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMD 557

Query: 559 YAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERRR 608
           YAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSPS PHW+PERRR
Sbjct: 558 YAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERRR 603

BLAST of PI0021589 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 903.7 bits (2334), Expect = 8.2e-263
Identity = 451/603 (74.79%), Postives = 510/603 (84.58%), Query Frame = 0

Query: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S   SP+      KD KALRFIEE T N D VQ++V+ EIL++N+ TEY+ RF L G T
Sbjct: 4   DSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DR TFK+K PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E
Sbjct: 64  DRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           +M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK
Sbjct: 124 DMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFK 183

Query: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQI 245
            RP DP+N YTSPNEA+LC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ 
Sbjct: 184 RRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTE-EWEGIITKI 305
           NWK+LA DISTGTL+ +I+D  ++E + SK LT P++ELA+FI + C  +  WEGIITKI
Sbjct: 244 NWKELADDISTGTLSSRISDPAIKESM-SKILTKPDQELADFITSVCGQDNSWEGIITKI 303

Query: 306 WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTI 365
           WPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTI
Sbjct: 304 WPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTI 363

Query: 366 MPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVL 425
           MPNM YFEFLPH       +        LV+LADVEVGKEYELVITTYAGL RYRVGD+L
Sbjct: 364 MPNMAYFEFLPHHEVPTEKS-------ELVELADVEVGKEYELVITTYAGLNRYRVGDIL 423

Query: 426 EVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYAD 485
           +VTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+
Sbjct: 424 QVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAE 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           TKTIPGHYVIYWELLVKD     + P DE++ +CCL +EESLNSVYRQ RVAD SIGPLE
Sbjct: 484 TKTIPGHYVIYWELLVKD---QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 605
           IRVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE
Sbjct: 544 IRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPE 595

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 595

BLAST of PI0021589 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 862.8 bits (2228), Expect = 1.6e-250
Identity = 431/599 (71.95%), Postives = 497/599 (82.97%), Query Frame = 0

Query: 10  SSPLGPPACEKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDT 69
           S    P   E + KAL+FIEE T N DSVQ++VL EIL++N++TEY+ RF LNGA DR +
Sbjct: 9   SGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKS 68

Query: 70  FKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMER 129
           FKSK PVV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI+E++ R
Sbjct: 69  FKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINR 128

Query: 130 RQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPY 189
           RQLL +LLMP+MNLYVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKSD F+T   
Sbjct: 129 RQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDS 188

Query: 190 DPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQINWKQ 249
           D  + YTSP EA+LC DS QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FLQ NWK+
Sbjct: 189 D--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKE 248

Query: 250 LAHDISTGTLNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWPNTK 309
           L+ DISTGTL+ KI D  ++  + S  L  P++ELAEF+   CS E WEGIITKIWPNTK
Sbjct: 249 LSQDISTGTLSSKIFDHAIKTRM-SNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTK 308

Query: 310 YLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMG 369
           YLDVIVTGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NLNPMCKPS+V+YTI PNM 
Sbjct: 309 YLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMA 368

Query: 370 YFEFLP--HDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVT 429
           YFEFLP  HD      A S      LV+LADVEVGKEYELVITTYAGL RYRVGD+L VT
Sbjct: 369 YFEFLPHNHDGDGGVEATS------LVELADVEVGKEYELVITTYAGLYRYRVGDILRVT 428

Query: 430 GFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKT 489
           GFHN+APQF+F+RR+NVLLSI+SDKTDEA+LQKAVENAS LL E  T V+EYTSYADTKT
Sbjct: 429 GFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKT 488

Query: 490 IPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRV 549
           IPGHYVIYWELL +D    N+ P+DE++ +CCL +EESLN+VYRQ RV+D SIGPLEIRV
Sbjct: 489 IPGHYVIYWELLSRDQS--NALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRV 548

Query: 550 VKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 607
           V+NGTFEELMD++ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW+PERR
Sbjct: 549 VQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

BLAST of PI0021589 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 796.2 bits (2055), Expect = 1.8e-230
Identity = 382/596 (64.09%), Postives = 473/596 (79.36%), Query Frame = 0

Query: 19  EKDAKALRFIEETTTNADSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVT 78
           EK+   L+FIE+ TTNAD VQ+RVL EIL++NA  EY+ R  L G TDR+TFK   PVVT
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 79  YEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLM 138
           YED+QP+I RIANGD+S +  S+PISEFLTSSGTS GERKLMPTI+EE++RR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 139 PIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSP 198
           P+M+ +VPGLDKGKG+YFLF+K+E+KTPGGL ARPVLTSYYKS  FK RPYDP+ +YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 199 NEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQINWKQLAHDISTGT 258
           N+ +LC+DS+QSMY+QMLCGL    +VLRVGAVFASG +RAI+FL+ +W +LA DI TGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 259 LNPKITDTCLRECITSKYLTNPNRELAEFIANECSTEEWEGIITKIWPNTKYLDVIVTGA 318
           L+ +ITD+ +RE +    +  P+ +LA+F+ +EC    W+GIIT++WPNTKY+DVIVTG 
Sbjct: 259 LSSEITDSSVREAVGE--ILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 318

Query: 319 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLP--- 378
           M+QYIPTL++YS GLP+ CTMYASSECYFGVNL P+CKPS+V+YT++PNM YFEFLP   
Sbjct: 319 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 378

Query: 379 ----HDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHN 438
                 S S   AL+      LVDL DV++G+EYELV+TTYAGL RYRVGDVL V GF N
Sbjct: 379 NSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 438

Query: 439 AAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGH 498
            APQF F+ RKNV+LSIDSDKTDE ELQ AV+NA + L  F+ ++ EYTSYADT +IPGH
Sbjct: 439 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 498

Query: 499 YVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNG 558
           YV++WEL +  N  +       +   CCL IEESLNSVYRQGRV+D SIGPLEI++V++G
Sbjct: 499 YVLFWELCLNGNTPI----PPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 558

Query: 559 TFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERRR 608
           TF++LMDYAIS GASINQYK PRCV F PI+ELL+SRV   +FSP  P W+P  ++
Sbjct: 559 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQ 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823332.7e-27476.78Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221901.2e-26174.79Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS01.0e-25472.88Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z61.0e-25472.88Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M21.1e-25171.04Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A5D3CNV80.0e+0098.52Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B0G20.0e+0098.52probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A0A0KJM50.0e+0097.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1[more]
A0A6J1GH540.0e+0092.52probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1IRM50.0e+0092.51probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
XP_008440335.10.0e+0098.52PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >... [more]
XP_004141893.10.0e+0097.69probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48572... [more]
XP_038881175.10.0e+0095.39probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
KAG6604151.10.0e+0092.69putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ... [more]
XP_023543141.10.0e+0092.69probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT2G14960.11.9e-27576.78Auxin-responsive GH3 family protein [more]
AT4G37390.12.2e-26876.57Auxin-responsive GH3 family protein [more]
AT2G23170.18.2e-26374.79Auxin-responsive GH3 family protein [more]
AT1G59500.11.6e-25071.95Auxin-responsive GH3 family protein [more]
AT5G54510.11.8e-23064.09Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 28..578
e-value: 2.5E-201
score: 670.1
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 10..607
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 10..607

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021589.1PI0021589.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity