PI0016847 (gene) Melon (PI 482460) v1

BLAST of PI0016847 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.8e-95
Identity = 182/371 (49.06%), Postives = 247/371 (66.58%), Query Frame = 0

Query: 3   DTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDS--MTLS 62
           D    E NK K+ +E++   + +WKY+FGF ++A  KCAI+LKI + IE+H  S  +TL+
Sbjct: 11  DRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLA 70

Query: 63  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRE----SYSHTPLSR--LLTTTGPNSMAP 122
           +LSS+++ SP  L RI+RFLVH+GIFKE  T++     Y +TPLSR  ++T     S+AP
Sbjct: 71  ELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAP 130

Query: 123 LHLLESSPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMA 182
             L E++P M+APW  LS+ V +  +     PF+  HG D+W++A  NP  S ++NE MA
Sbjct: 131 FVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMA 190

Query: 183 CFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS 242
           C AR V+ P V   C  +FDGV  +VDVGGG G  + +LVK  PWIKG NFDLPHV+ V+
Sbjct: 191 CDARRVV-PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 250

Query: 243 QEYEGVQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEV 302
           +  +GV++V G+MFDS+P  DA FI  VLHDW D++CIKIL+NCKEA+P   GKV+IVE 
Sbjct: 251 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 310

Query: 303 VIDEK-------EEISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTP 359
           VI E        E   K   VRLM+DM+MMAHT  GKERT +EW  VL++AGF+ Y    
Sbjct: 311 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 370

BLAST of PI0016847 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 155/352 (44.03%), Postives = 220/352 (62.50%), Query Frame = 0

Query: 22  KVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLL----LHRIL 81
           +  VWK I G  +  V+KCA+EL+I D++ SH   +TL+Q++SS+  SP L    L RI+
Sbjct: 16  EANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSRIM 75

Query: 82  RFLVHRGIFKEEKTRES----YSHTPLSRLL--TTTGPN--SMAPLHLLESSPVMVAPWH 141
           R LV R IF + K+ +     Y  T  SRLL   TT P+  ++AP     + P + APW 
Sbjct: 76  RLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWS 135

Query: 142 NLSACVKANSHEDGSQPFEMAH-GMDLWTYAAANPSHSSLVNEGMACFARLVILPAVLER 201
            L+ CVK     +G   FEM H G  LW  +  NP  + + N+GMA  AR+  + A+L  
Sbjct: 136 CLARCVK-----EGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTM-AILSE 195

Query: 202 CGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEG-VQHVGGNM 261
             ++F G+  LVDVGG  G  +S +VK+ P IKGIN+DLPHVV+ +  Y G V HVGGNM
Sbjct: 196 YRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNM 255

Query: 262 FDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKEEISKYSD 321
           F+ +P A A F+  +LHDW DE+C+KIL+NC+ A+PEK GK+IIV++V+ E E    + D
Sbjct: 256 FEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVL-EPEGNGLFDD 315

Query: 322 VRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
             +M+D+ +MA T +GKERT++EW  VL++ GF  Y       + SVI+A+P
Sbjct: 316 AAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

BLAST of PI0016847 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.4e-70
Identity = 146/356 (41.01%), Postives = 215/356 (60.39%), Query Frame = 0

Query: 16  EEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSL-----NCSP 75
           EE    + ++W+++F FV+   +KCA+EL I D+I SHG  +T+S++  SL     + SP
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 76  LL--LHRILRFLVHRGIFKEEKTRES----YSHTPLSRLLTTTGPNSMAPLHLLESSPVM 135
            +  L RI+R LVH+ +F  E  +ES    Y+ T  S+ L      +++PL L E++P++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 136 VAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVILPA 195
           + PW  L  C      ++ S PFE AHG ++W  A A+P  ++ +N  M C    +I   
Sbjct: 125 LKPWQYLGKCA-----QEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEM 184

Query: 196 VLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQHVG 255
           +LE         G LVDVGGG G+ ++ +VKA P I+GINFDLPHVV+ + E+ GV+HVG
Sbjct: 185 LLEYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVG 244

Query: 256 GNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAI-PEKTGKVIIVEVVIDEKEEIS 315
           G+MF  +P+ADA  +  +LHDW DE+C  IL+NC  AI  +K GKVIIV+ V+   +   
Sbjct: 245 GDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVL-RPDGND 304

Query: 316 KYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            +  + L+ D++MMAHT  GKERT+ EW  +L  AGF  Y    T     +I+AFP
Sbjct: 305 LFDKMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of PI0016847 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.7e-69
Identity = 147/351 (41.88%), Postives = 211/351 (60.11%), Query Frame = 0

Query: 14  IEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLL- 73
           + + D   + ++WK I+GF E  V+KCA++L+I + + ++   M+LS+L+S L  +  + 
Sbjct: 4   VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 74  ---LHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLESSPVMVAPWH 133
              L RI+R+LVH  +FK + T + YS  P ++ L      SM    L  +    +APWH
Sbjct: 64  EDRLFRIMRYLVHMELFKIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123

Query: 134 NLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVILPAVLERC 193
           +L   +  N        FE A G  +W Y + NP  + L N  MAC  RLV   A+   C
Sbjct: 124 HLGDGLTGN-----CDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLV-TSALANEC 183

Query: 194 GEIF-DGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQHVGGNMF 253
             IF DG+  LVDVGGG GT +  + KA P IK   +DLPHV++ S E   +  + G+MF
Sbjct: 184 KSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMF 243

Query: 254 DSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDV 313
            S+P ADA F+  +LHDW+D+ECI+IL+ CKEA+P K GKVIIV+VVID  +    Y+ +
Sbjct: 244 KSIPSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVID-MDSTHPYAKI 303

Query: 314 RLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
           RL +D+ MM +T  GKERTKEEW  +   AGF+ +  T  S +QSVI+A+P
Sbjct: 304 RLTLDLDMMLNT-GGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

BLAST of PI0016847 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 4.0e-68
Identity = 140/345 (40.58%), Postives = 210/345 (60.87%), Query Frame = 0

Query: 22  KVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSL---NCSPLLLHRILR 81
           + Q+W  I+GF +  V++CA+E+ I D+I+++  ++TL+QL++ L   N S   L+R++R
Sbjct: 20  QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 82  FLVHRGIFKEEK----TRESYSHTPLSRLLTTTGPNSMAPLHLLESSPVMVAPWHNLSAC 141
           +LVH  I ++E       + YS  P+  LL      SM P+ L  +    +  WH     
Sbjct: 80  YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH----F 139

Query: 142 VKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVILPAVLERCGEIFD 201
           +K       +  FE   GMD+W Y   NP  S L NEGMA   RL +   ++E C + F 
Sbjct: 140 MKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL-LTKTLIEDCRDTFQ 199

Query: 202 GVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQHVGGNMFDSVPKA 261
           G+  LVD+GGGNGT +  + +A P IK   +DLPHVV+ S +   ++ V G+MF SVP A
Sbjct: 200 GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSA 259

Query: 262 DAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDVRLMMDM 321
            A  +  +LHDW DEEC+ IL+ CKEAIP++TGKVIIV+V ++E E   + +  RL++D+
Sbjct: 260 QAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEE-ESNHELTKTRLILDI 319

Query: 322 IMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            M+ +T  G+ERT ++W N+L++AGF  +   P   IQSVI+AFP
Sbjct: 320 DMLVNT-GGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

BLAST of PI0016847 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 5.2e-196
Identity = 338/359 (94.15%), Postives = 350/359 (97.49%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDT SK SNKLKIEEEDSDIKVQ+WKYIFGFVEMAV+KCAIELKIGDVIESHGDSMTLS
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSLNCSPLLLHRILRFLVHRGIFKEEKT + YSHTP+SRLLTTTGPNSMAPLHLLES
Sbjct: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKCYSHTPMSRLLTTTGPNSMAPLHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKA+ HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NE MACFARLV
Sbjct: 121 SPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLV 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEG+
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGI 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKIL+NCKEAIPEKTGKVIIVEVVIDEKE
Sbjct: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 300

Query: 301 EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
           EISKYSD RLMMDMIMMAHTIKGKERT EEWANVLQKAGFS+YT TPTSTIQSVIQAFP
Sbjct: 301 EISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

BLAST of PI0016847 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.1e-193
Identity = 335/360 (93.06%), Postives = 353/360 (98.06%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDTG+KESNKLKIEEE+SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSL+CSPLLLHRILRFLVHRGIFKEEK+ +SYSHTP+SRLLTTTGPNSMAP+HLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKAN HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NEGMACFARL 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGG+MFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVE+VIDEKE
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            +ISKYSDV+L MDMIMMAHTIKGKERT EEWAN+LQKAGFS+YT TPT+TIQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

BLAST of PI0016847 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.1e-193
Identity = 334/360 (92.78%), Postives = 353/360 (98.06%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDTG+KESNKLKIEEE+SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDS+TLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSL+CSPLLLHRILRFLVHRGIFKEEK+ +SYSHTP+SRLLTTTGPNSMAP+HLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKAN HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NEGMACFARL 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGG+MFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVE+VIDEKE
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            +ISKYSDV+L MDMIMMAHTIKGKERT EEWAN+LQKAGFS+YT TPT+TIQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

BLAST of PI0016847 vs. ExPASy TrEMBL
Match: A0A6J1GLV0 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455162 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.3e-158
Identity = 284/359 (79.11%), Postives = 324/359 (90.25%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEED-SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTL 60
           MD+ GSK+S KLKIEEE+ +DIKVQVWKYIFGFVEMAV+KCAIELKIGD IE+HG  MTL
Sbjct: 1   MDNIGSKDS-KLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTL 60

Query: 61  SQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLE 120
           + LSSSLNCSPLLLHRILRFL+HRGIFKEE T+ SY+ TP+SRLLT++G +S+AP+H+LE
Sbjct: 61  TDLSSSLNCSPLLLHRILRFLIHRGIFKEEPTK-SYAQTPMSRLLTSSGAHSLAPMHVLE 120

Query: 121 SSPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARL 180
           SS VM+APWHNLSA +KAN  + GSQPFEMAHG DLW Y AAN SH+ ++NEGMA FAR+
Sbjct: 121 SSHVMLAPWHNLSAYLKAN--DGGSQPFEMAHGKDLWRYCAANSSHNKVMNEGMASFARV 180

Query: 181 VILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEG 240
           VI+PAVLERCG +F+GVG LVDVGGGNG+CLS+LVKA PWI+GINFDLPHVVSVSQEYEG
Sbjct: 181 VIVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEG 240

Query: 241 VQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEK 300
           V+HVGGNMFD VPKADAAFIM  LHDWDDE+CIKIL+NCKEAIPEKTGKVIIVEVVIDEK
Sbjct: 241 VRHVGGNMFDFVPKADAAFIMNALHDWDDEDCIKILKNCKEAIPEKTGKVIIVEVVIDEK 300

Query: 301 EEISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAF 359
           +E S  SD+RLM+DMIMMAHTIKGKERT+EEWA VLQ+AGFS +T TPTSTIQS+IQ F
Sbjct: 301 DE-SMVSDIRLMLDMIMMAHTIKGKERTREEWAYVLQQAGFSRHTITPTSTIQSIIQGF 354

BLAST of PI0016847 vs. ExPASy TrEMBL
Match: A0A6J1HWX9 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.9e-154
Identity = 277/358 (77.37%), Postives = 318/358 (88.83%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MD+ GSK+S K K EEE +DIK+QVWKYIFGFVEMAV+KCAIELKI D I++HG  MTL 
Sbjct: 1   MDNIGSKDS-KFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLP 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           +LSSSLNCSPLLL RILRFL+HRGIFKEE  + SY+ TP+SRLLT++G +S+AP+H+LES
Sbjct: 61  ELSSSLNCSPLLLRRILRFLIHRGIFKEEPIK-SYAQTPMSRLLTSSGAHSLAPIHVLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           S VM+APWHNLSA +KAN+   G+QPFEMAHG DLW+Y AAN SH+ ++NEGMA F+R+V
Sbjct: 121 SLVMLAPWHNLSAYLKANN--GGTQPFEMAHGKDLWSYCAANSSHNMVMNEGMASFSRVV 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           I+PAVLERCG +F+GVG LVDVGGGNG+CLS+LVKA PWI+GINFDLPHVVSVSQEYEGV
Sbjct: 181 IVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           +HVGGNMFD VPKADAAFIM  LHDWDDEECIKIL+NCKEAIPEKTGKVIIVEVVIDEKE
Sbjct: 241 RHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 300

Query: 301 EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAF 359
           E S  SDVRLM+DMIM+AHTIKGKERT+EEWA VLQ AGFS +T TPTSTIQS+IQ F
Sbjct: 301 E-SMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGAGFSRHTITPTSTIQSIIQGF 353

BLAST of PI0016847 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 338/359 (94.15%), Postives = 350/359 (97.49%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDT SK SNKLKIEEEDSDIKVQ+WKYIFGFVEMAV+KCAIELKIGDVIESHGDSMTLS
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSLNCSPLLLHRILRFLVHRGIFKEEKT + YSHTP+SRLLTTTGPNSMAPLHLLES
Sbjct: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKCYSHTPMSRLLTTTGPNSMAPLHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKA+ HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NE MACFARLV
Sbjct: 121 SPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLV 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEG+
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGI 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKIL+NCKEAIPEKTGKVIIVEVVIDEKE
Sbjct: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 300

Query: 301 EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
           EISKYSD RLMMDMIMMAHTIKGKERT EEWANVLQKAGFS+YT TPTSTIQSVIQAFP
Sbjct: 301 EISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

BLAST of PI0016847 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 684.1 bits (1764), Expect = 6.5e-193
Identity = 335/360 (93.06%), Postives = 353/360 (98.06%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDTG+KESNKLKIEEE+SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSL+CSPLLLHRILRFLVHRGIFKEEK+ +SYSHTP+SRLLTTTGPNSMAP+HLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKAN HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NEGMACFARL 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGG+MFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVE+VIDEKE
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            +ISKYSDV+L MDMIMMAHTIKGKERT EEWAN+LQKAGFS+YT TPT+TIQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

BLAST of PI0016847 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 682.6 bits (1760), Expect = 1.9e-192
Identity = 334/360 (92.78%), Postives = 353/360 (98.06%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MDDTG+KESNKLKIEEE+SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDS+TLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           QLSSSL+CSPLLLHRILRFLVHRGIFKEEK+ +SYSHTP+SRLLTTTGPNSMAP+HLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SPVM+APWHNLSACVKAN HE+GSQPFEMAHGMDLWTYAAANPSHSSL+NEGMACFARL 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGG+MFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVE+VIDEKE
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
            +ISKYSDV+L MDMIMMAHTIKGKERT EEWAN+LQKAGFS+YT TPT+TIQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

BLAST of PI0016847 vs. NCBI nr
Match: XP_038888743.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-179
Identity = 310/359 (86.35%), Postives = 338/359 (94.15%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60
           MD+ GSKESNKLKIEEE+SD+KV+VWKYIFGFVEMAV+KCAIELKIGD IES+G SMTLS
Sbjct: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60

Query: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLES 120
           +LSSSLN SPLLLHR+LRFLVHRGIFKEEK  +SYS TP+SRLLTTTGPNS+AP+HLLES
Sbjct: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLES 120

Query: 121 SPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLV 180
           SP MVAPWH LSAC+KAN HE+ SQPFE+AHGMDLW+YA+ANPSH+ LVNE MACFAR+V
Sbjct: 121 SPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVV 180

Query: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGV 240
           ILPAVL+RCGEIFDGVG LVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS SQEYEGV
Sbjct: 181 ILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGV 240

Query: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKE 300
           QHVGGNMFDS+PKADAAFIMEVLHDWDDEECIKIL+N KEAIP+KTGKVIIVEVVIDE E
Sbjct: 241 QHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENE 300

Query: 301 EISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAFP 360
           +IS+ SD+RLMMDMIMMAHTIKGKERT  EWANVLQKAGFSHYT TPT++IQSVIQAFP
Sbjct: 301 KISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 359

BLAST of PI0016847 vs. NCBI nr
Match: KAG6572137.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.0 bits (1460), Expect = 1.2e-157
Identity = 283/359 (78.83%), Postives = 325/359 (90.53%), Query Frame = 0

Query: 1   MDDTGSKESNKLKIEEED-SDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTL 60
           MD+ GSK+S KLKIEEE+ +DIK+QVWKYIFGFVEMAV+KCAIELKIGD IE+HG  MTL
Sbjct: 1   MDNIGSKDS-KLKIEEEENNDIKIQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTL 60

Query: 61  SQLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRESYSHTPLSRLLTTTGPNSMAPLHLLE 120
           ++LSSSLNCSPLLLHRILRFL+HRGIFKEE T+ SY+ TP+SRLLT++G +S+AP+H+LE
Sbjct: 61  TELSSSLNCSPLLLHRILRFLIHRGIFKEEPTK-SYAQTPMSRLLTSSGAHSLAPMHVLE 120

Query: 121 SSPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARL 180
           SS VM+APWHNLSA +KAN  + GSQPFEMAHG DLW Y AAN SH+ ++NEGMA FAR+
Sbjct: 121 SSHVMLAPWHNLSAYLKAN--DGGSQPFEMAHGKDLWRYCAANSSHNKVMNEGMASFARV 180

Query: 181 VILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEG 240
           VI+PAVLERCG +F+GVG LVDVGGGNG+CLS+LVKA PWI+GINFDLPHVVSVSQEYEG
Sbjct: 181 VIVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEG 240

Query: 241 VQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEK 300
           V+HVGGNMFD VPKADAAFIM  LHDWDDE+CIKIL+NCKEAIPEKTGKVIIVEVVIDEK
Sbjct: 241 VRHVGGNMFDFVPKADAAFIMNALHDWDDEDCIKILKNCKEAIPEKTGKVIIVEVVIDEK 300

Query: 301 EEISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSVIQAF 359
           +E S  SD+RLM+DMIMMAHTIKGKERT+EEWA VLQ+AGFS +T TPTSTIQS+IQ F
Sbjct: 301 DE-SMVSDIRLMLDMIMMAHTIKGKERTREEWAYVLQEAGFSRHTITPTSTIQSIIQGF 354

BLAST of PI0016847 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.7e-96
Identity = 182/371 (49.06%), Postives = 247/371 (66.58%), Query Frame = 0

Query: 3   DTGSKESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDS--MTLS 62
           D    E NK K+ +E++   + +WKY+FGF ++A  KCAI+LKI + IE+H  S  +TL+
Sbjct: 11  DRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLA 70

Query: 63  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTRE----SYSHTPLSR--LLTTTGPNSMAP 122
           +LSS+++ SP  L RI+RFLVH+GIFKE  T++     Y +TPLSR  ++T     S+AP
Sbjct: 71  ELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAP 130

Query: 123 LHLLESSPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMA 182
             L E++P M+APW  LS+ V +  +     PF+  HG D+W++A  NP  S ++NE MA
Sbjct: 131 FVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMA 190

Query: 183 CFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVS 242
           C AR V+ P V   C  +FDGV  +VDVGGG G  + +LVK  PWIKG NFDLPHV+ V+
Sbjct: 191 CDARRVV-PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 250

Query: 243 QEYEGVQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEV 302
           +  +GV++V G+MFDS+P  DA FI  VLHDW D++CIKIL+NCKEA+P   GKV+IVE 
Sbjct: 251 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 310

Query: 303 VIDEK-------EEISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTP 359
           VI E        E   K   VRLM+DM+MMAHT  GKERT +EW  VL++AGF+ Y    
Sbjct: 311 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 370

BLAST of PI0016847 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 298.5 bits (763), Expect = 7.1e-81
Identity = 164/364 (45.05%), Postives = 218/364 (59.89%), Query Frame = 0

Query: 8   ESNKLKIEEEDSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDS--MTLSQLSSS 67
           E +K  + +E++   + +W+Y+FGF ++A  KCAI+LKI + IE+H  S  +TLS+LSS+
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 68  LNCSPLLLHRILRFLVHRGIFKEEKTRE----SYSHTPLSRLLTTTGPNSMAPLHLLESS 127
           ++ SP  L RI+RFLVH+G+FKE  T++     Y++TPLSR +  T              
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMIT-------------- 121

Query: 128 PVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVI 187
                                        HG DLW +A  N  HS L+NE MAC AR V+
Sbjct: 122 ---------------------------KLHGKDLWAFAQDNLCHSQLINEAMACDARRVV 181

Query: 188 LPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQ 247
            P V   C  +FDGV  +VDVGGG G  + ILVK  PWIKG NFDLPHV+ V+Q  +GV+
Sbjct: 182 -PRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVE 241

Query: 248 HVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEK-- 307
           +V G+MFDS+P +DA  I  VLHDW D++CIKIL+NCKEA+    GKV+IVE VI EK  
Sbjct: 242 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 301

Query: 308 -----EEISKYSDVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGFSHYTFTPTSTIQSV 359
                E   K   VRL +DM+MM HT  GKERT +EW  VL +AGF+ Y       +QS+
Sbjct: 302 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSL 323

BLAST of PI0016847 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 188.7 bits (478), Expect = 8.0e-48
Identity = 111/315 (35.24%), Postives = 179/315 (56.83%), Query Frame = 0

Query: 38  VKCAIELKIGDVIESHGDSMTLSQLSSSLNC----SPLLLHRILRFLVHRGIFKEEKTRE 97
           +K A+EL + +++  +G  M+ ++++S L      +P++L RILR L    +      + 
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 98  S-------YSHTPLSRLLTTTGPN-SMAPLHLLESSPVMVAPWHNLSACVKANSHEDGSQ 157
           S       Y   P+ + LT      S+A L L+    V++  W++L   +      DG  
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAIL-----DGGI 155

Query: 158 PFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVILPAVLERCGEIFDGVGCLVDVGGG 217
           PF  A+GM  + Y   +P  + + N GM+  +  + +  +LE   + F+G+  LVDVGGG
Sbjct: 156 PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKILETY-KGFEGLTSLVDVGGG 215

Query: 218 NGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQHVGGNMFDSVPKADAAFIMEVLHD 277
            G  L ++V   P +KGINFDLPHV+  +  + G++HVGG+MF SVPK DA F+  + HD
Sbjct: 216 IGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHD 275

Query: 278 WDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDVRLMMDMIMMAHTIKGKE 337
           W DE C+K L+NC E++PE  GKVI+ E ++ E  + S  +   + +D IM+AH   GKE
Sbjct: 276 WSDEHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKE 335

Query: 338 RTKEEWANVLQKAGF 341
           RT++E+  + + +GF
Sbjct: 336 RTEKEFEALAKASGF 342

BLAST of PI0016847 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.4e-44
Identity = 123/351 (35.04%), Postives = 191/351 (54.42%), Query Frame = 0

Query: 15  EEEDSD---IKVQVWKYIFGFVEMAVVKCAIELKIGDVI--ESHGDSMTLSQLSSSLNC- 74
           EE++ D   + +Q    +       V+K A+EL + D I   S+G  ++ S+++ SL   
Sbjct: 20  EEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNK 79

Query: 75  -----SPLLLHRILRFLVHRGIFK-----------EEKTRESYSHTPLSR--LLTTTGPN 134
                +P+LL R+LR LV   I K             K    Y+  P+ +  L  + G  
Sbjct: 80  PTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSG 139

Query: 135 SMAPLHLLESSPVMVAPWHNLSACVKANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVN 194
           S++ L LL  S V++  W NL   +      +G   F  AH M L+ Y +++   S L +
Sbjct: 140 SLSSLLLLLHSQVILKTWTNLKDVIL-----EGKDAFSSAHDMRLFEYISSDDQFSKLFH 199

Query: 195 EGMACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHV 254
             M+  + +V+   + E  G  F+ V  LVDVGGG GT L ++    P IKG+NFDL  V
Sbjct: 200 RAMSESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQV 259

Query: 255 VSVSQEYEGVQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVI 314
           ++ +  Y GV+HV G+MF  VPK DA F+  +LHDW DE+CIKIL+NC +++PEK GKVI
Sbjct: 260 LTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKVI 319

Query: 315 IVEVVIDEKEEISKYS-DVRLMMDMIMMAHTIKGKERTKEEWANVLQKAGF 341
           IVE++   + + + +S +  L MD++M+     GKER+  ++ N+   +GF
Sbjct: 320 IVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGF 362

BLAST of PI0016847 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.7e-43
Identity = 115/326 (35.28%), Postives = 174/326 (53.37%), Query Frame = 0

Query: 37  VVKCAIELKIGDVIESHGDSMTLS--QLSSSLNC------SPLLLHRILRFLVHRGIFK- 96
           V+K A EL + D I + G+   LS  +++ SL        +P+LL R+L  LV   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 97  ----------EEKTRESYSHTPLSR--LLTTTGPNSMAPLHLLESSPVMVAPWHNLSACV 156
                       K    Y+  P+ +  L  + G  S+ PL +L  + V    W NL   +
Sbjct: 105 RMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVI 164

Query: 157 KANSHEDGSQPFEMAHGMDLWTYAAANPSHSSLVNEGMACFARLVILPAVLERCGEIFDG 216
                 +G   F  AHGM ++ Y  ++   + L N  M+  + +++   +    G  F+ 
Sbjct: 165 L-----EGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYRG--FED 224

Query: 217 VGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSQEYEGVQHVGGNMFDSVPKAD 276
           V  LVDVGGGNGT L ++    P IKG+NFDL  V++ +  Y GV+HV G+MF  VPK D
Sbjct: 225 VNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGD 284

Query: 277 AAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEVVIDEKEEISKY-SDVRLMMDM 336
           A F+  +LHDW DE+CIKIL+NC +++PEK GK+IIVE V  ++ +     S+    MD+
Sbjct: 285 AVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDL 344

Query: 337 IMMAHTIKGKERTKEEWANVLQKAGF 341
           +M+     GKER+  ++ N+   +GF
Sbjct: 345 LMLTQCSGGKERSLSQFENLAFASGF 362