PI0016141 (gene) Melon (PI 482460) v1

Overview
NamePI0016141
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionlipase-like PAD4
Locationchr08: 12554263 .. 12560054 (+)
RNA-Seq ExpressionPI0016141
SyntenyPI0016141
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCCGAAGCTTCAACGTAATTCATTCAACTTTTTCTTTTTCTTTTCTACTTTCAATTTTTTCAACTCTCTTTTCAATTTCCTTTCTTTTCTTTCCAGATTCGAGAGTTGTCATGTCATGGCTGCTCTTTTGGGTTCAACTCCTCTGCTTCTGCAGTCGTGGGAATTCTGCGCCGCCGCCAACGCCACCTCCCCAGAAAGTTTCACGACTGTGGTGATTGATGATGTGGCTTATGTTGCCTTCTCCGGCGTCCAAGTTCTTGGTAGATGCGGCGGGGGAAGAGAGTTGGTGGCGTTGGATGGAGAGGGAGTTGAGGGAGAGCTGTTTTGGCCGTTGAACCGCCACCGTGAGGAGCTCCAGGAAGCGGCCATGGCGGATTCCGGCATCCTCAAGATGTTTGTTGATATTTATACTCATAAGAATCTTGTTGAAACGGTCAGTTTTCAAATCTCTTACCTAATTCTTTACCATTTACTTTCTACCCCACTTTGACTGAATTGGAGTTTTTAAATGTTCAAACAAAGTATTTATAACTCAAATAAAATGTATATAAATAGTGAAACTCAATCCAAACAAAATCCTAATTTTCTACCTCAAATTACATTCACTAATCATTTAATTTTTACTCTTTATTTTTTTTTCTAAATTTTTTTATTTTTTATTTAGTTTTTTACCATGTTTTCACAAATCAAACCCAATTTTAAAATCTTAAAAAATAGCTTTTTAAAAAGCTTGTTTTTCTCTTTAAAATTTGATCAAAAACTCAACTAATTTACCTTAAAAAAAATGAAGAAATTGATAAAAGAAAAAAATAGACTTAGCTTTTAAAAACTAAAATTCAATAACAAAAAGTTACTAAATAAGTCGTAATTTTTAAAAATCTGTTTTTATTTTTTCTACTTTAGTTAAGCATTGTTTTTTACTTAAAAGAAAATGAAAACTAAATGGATTCGATTTCAAAAAAAAAACTCTAAACTAAACAAAACCTTATTATATACTCTTGAGATTAGGGTTTGGATTCTATCCTTTCATATATTCTTAAAAGAATTGTTTATATGTTTTGATAATTATTTCAAAGGATAGTGTTTGTACTTTTTGAACCATGATTAATATATTTATCGATATATGTGTATGCTAAAGAGTTTAACATCAAAATATGGAATATAATTGGATAATATCATTCATATTTTGTATAAATACCCTTAAAATATAAAAATGTATCATCAATTTATCACCAATATAACTATATAAATTCTAATAGTTATTAAAATTTACGACTTATTTAGAGACTAAAAATAATGATAATTGTTATGGCTTTCGATTGAATCCCTCGTAGTTGTTACATTCAAAATAATAATTTAAGTCGTATTTGATAACTCCTTGAAATTTTTGTTTTTAGTGATTAGGCCTATAAACATTTATTCAAACTCTAAATTTTTTTGTTTTGTTATAAATTTTTATCAATGTTTTCAAAAACCAAGCCAAAATTTGAAAACTAAAAAAATTGTAGTTTTACTAATTTTTGTTTTTGAAATTTAGCTAGAAAATTAACTCTTTTAAATTAAGGAAAAAGCAAATCATAATTAAGAAATTAGGAGGAATTAGGCTTTTTTTTTTTTTTCGAAAACCAAAAATAAAAAACACAATGGTTACTAAACGTGTGTTCAGGAGTCTTTTGTTCTATGAGCATTCTAAATTCTCATAGAATGGATATCTGGACTACCATGTTTGTCTTGCGATACTATAAAAAGCTATGTTATTTGAAGATTTCATGCATCTCAAAAGTTTTTGGATGTTTTATTGAAACATGGGTTTTCCAGATTTTGTAGGTAGGGCATTTCCATGTTTACATTTTTCTTGCCTTTCTCTTTTCCTTATGTATATATAACTATCCATTTGCATTATATAATTAAAATACATGTTGTCAACTATCTATATCATTTTTTTTTTTTTTTGGTTATATTTTAGTTCTTATAAATTTTTAAGCAAGATGTTCAAATTCAAATTTAAATTAATATGCATTTTATAAAAAAAATCTTAATTTATGATATATATATAAACATATATAAATTAGTTTGTCCAGAAATACAAAATTATTAAAAACAAATATCTTATATAAGTTTAAATTATATTAAATTAATTTTAAATGAATAAATAGTCATAATATTATTGTATAAATTTAAGATATATTCGAGTAGAAATCTTACAAACAAATAAAGTTATCAAAATATTTTCAGATGTTTGCAAAAACATTCCTACAAAACAAACATATTAATCCAAAGATGTTGGATATCTAAAGTTTCGAGTATCTAAAAATTCCTATTATTTACCTTATAAGAAAAACAAACAGTCCCTCAAAGATTCCCACATAAATGTCATAAACATTTTCTACATTTTACATCTAAATATTTTTTAGAAAAATTATTTATATAACAAAACTGTTAAAAATACGTACAAATATAACAAAATATAATTTTAACGATAAGAAGTAAAATTTTACTTTATTTATAAATGTTTTTCATTTTACTATATTAAAAAACAACTTGTTTATGTGAGAAAAACTAAAGAAATAAGATATAAAATCGATCCTTAAAATAAATAAATAAATAAATATATATATATATATATATATATAATCATAAAATTAATAATATTATTTACGTTTATAATTAATTATTTGAATTATGAAATTTTTGCCCGTTTTGTGGTCTTGTTGACAAAAGTCTTGGAAAGGGACTTAGGAAGTCCACCATTAATTCACCAAATATTACTTTCAAGTTTGAATGGAAAAATGAATATCATTTAAATTCAGTTGTATTATTATTATTATTATTGTTGTTGTTGTTTCTTTGTTATTGTAATTTATTTTTATTGTAGTATGAGATTCATTTATACTTTTATTTTTTGTAAGATTGTTTTTCATTGATGACATCTTGTTTAATAATCTACTCTTTTTTCAAATATCTTAACCAAATATTCAAAACTCATCTTTGACCATTGACTTCAAAAGCAATTTTATATCAAGGGTTGATGATTTTTTAAAAAAAAAATAATAATAAACAAAAAAAAAAGGAGAAATAAAGGAATTTCGAACCTTTTCTAAATATGCTTTCTTAAGAACTTATTACATGAGACTTATATTGACGAAAATAATATTAATACTCATTTATTATGTACAATGAAAGTATATATATGATATTAGAAAAAGCCACTAGAAACTTTTGATACTGAGCAAGATGTAGATAAATAATGTTGGAATTTCTTTCTAACCACTTTTTTCTCTACCAACCTTTGCCCAATGTCAATATAATTAACGAAATGTTCTTTTCTTTTTTTGAAACTTGGAATATGTTTATATTAAAGTTTCATTGGAAAAGAAATTAATGATTGATTGCAAAAGGTAGTGTGAAAAGTGAAGCTTCTTTGGACCAAAATATAATTGGAAATTTAAAGGAAATTACATATGAGAAACTTAGATGGGTACATTTGGACCATTCTTTACTAATCATTTTTTAATGATTAAACAAATTCAATATGGGTCTAGTCATCATTTGGTTTTTGGTTTCCTATATTTAAAATTGATCCTACAACCACTCATTCCATTTGTTGCTTTATATATTTTGTTGCTTTATATATTTTGTTGTTTACTTTTAGAAACATTTTTAAAATCAATGCAAAATCTTGAAAACAAATTTATATATATATATATATATATAGTTTTCAAAGACTTATCTTTGTTTTTAGAAATTAACTAAGGAATCAAATAAATGTCTACATGGAAATATGAGAACCGTTAGTAAGAAATTGTAAGAAAACATGCAAGATTTTCAAAAACCAAATATAAGAGCCCTTAGTAAGAAATGTAGAAATGTATCTAATGTACCATGTAGGTTTAAGAATTGTTTCAATTTGACCCCTAAATTTCCAATTTTACACTTTTTAACCTCAATTTATCACAAATCTTAAAACTCAAGGACTAAATTAAAAAACAAAATTCAAAACTCATTTTCAAACTTTAGAACCAAATAAAAACCATTAGGGACCAAAAGGGGCAAGTAAAAGATCATCTTTTTGGTTCCAATGGTTTACTTTAAACCTTTCCCCATTCAGTGTTTCAGTTCATTACTTTCTTTTGAACTTTGGCTTAGAAAGGCCTAAAGACTTTCCAATGAGAGGCCTATGATCTATACATACATATTTGTTTTGAAAAGTATTATTGGTATATATGTTGCAGATGACAAAGGTAATGGAGAAAAGCAAGTCAATAGTAATTACAGGTCATTCACTTGGAGGAGCAGCAGCCACTCTATGTACACTTTGGCTGCTTTCTTTCTTCCATACCAAAACCCATCATCACCCAATTCTATGCATCACCTTTGGCTCCCCATTGATAGGCAATGAGTCACTTTCTAGAGCCATTCAAAGAGAACGATGGTGCGGCAAGTTTTGCCATGTCGTCTCCAACTACGACATTATGCCAAGGCTTCTCTCTACTCCACTTTCCTCTCTCTCCCCTAAGCTCCATATCCTCTTTAGATATTGGCATTTGTCAATGGCCTCTCCACATTTTGGGAAGCTTGCTACCCAATTGACAGAAAGAGAGAAAGAAGAGCTCTTCCACGTCGTATTGGCTCACTCCAACTCGATATCCGACCTAGGAGAGGGATCGGTACGAGGTCAGTTTTGGCCATTTGGAAACTTCTTCTTTTGCTCGGAACACGGTGCAATTTGTTTGGATAATGCCATGTCAGTTCTTAAGATGCTCTATCTGATGCTCAAAACAAGTGCTCCAAACTTGAGCATTGAGGACCATCTCAACTATGGAGATCATGTGAGAAAAGTTGGAGTTCAATACATGGAGAGGAAGAACTTCAATTCATTGAGTCTTCCTAATTCAAGCTATGAAGCTGGGCTTGCTCTAGCATTGCAGTCAGCAGGAATACCTTTCCAAGTAACAAGCCTTAAGAACTTTCCTTAAAAACAAAACCTACAACTTAGATTTTAGAACTAACTGGAGCTTATTTCAAAATTGCAGGATGAAGTTGCTCGAATGGCCGAACATAGCTTGAGAACAGCAAGCAGAATAGGACAAACACCAAACTTGAATGCGGCAAAACTAGCTATAAGCTTATCAAAGATTACACCTTACAGGGCTGAGATAGAATGGTACAAAGCCTCTTGCGAGGAGGCAGATAACCAATTGGGTTACTACGATTGCTTCAAGAAAGAAGATGCTTCGGTAAGACATGACCGAGTAAATATGAACAGGCACAAGCTTGCAACATTCTGGAACAGAGTGATCGACATGTGGGAGAACAATGAGCTTCCTCCAGATTTTAACACAAGGGCAAAGTGGGTCAATGCTTCACAATTCTACAAACTCCTGGTAGAGCCACTGGACATTGCAGAATATTACCGTCGTGATAACCACATTGTCCATGGTCATTATTTGAATAGCGGAAGAGAGAGAAGATATGAGATTTTTGACAAATGGTGGAGAGGAAGAGAAGTCACAGAGGAAGGGAACACTCAGAGGATGAAATATGCAAGCTTGACCCAAGACTCATGCTTTTGGGCAAGGTTGGAGGAAGCCAAAGACCTACTGGAAATCATTAAAAGAGAAGGAGATGTGAGAAAGTTGGCTCCAATATGGAAAAGTCTTGAAAACTTTGAAAGGTATGCCAGAGGATTGATTGAAAGAAAGGAGGTTTCTAAAGATGTCATAGCAAAGAATTCAAGCTACACTTTGTGGGCTCAAGAACTGAGGGCATTGAAGCTAAATATGTAA

mRNA sequence

ATGGAGTCCGAAGCTTCAACATTCGAGAGTTGTCATGTCATGGCTGCTCTTTTGGGTTCAACTCCTCTGCTTCTGCAGTCGTGGGAATTCTGCGCCGCCGCCAACGCCACCTCCCCAGAAAGTTTCACGACTGTGGTGATTGATGATGTGGCTTATGTTGCCTTCTCCGGCGTCCAAGTTCTTGGTAGATGCGGCGGGGGAAGAGAGTTGGTGGCGTTGGATGGAGAGGGAGTTGAGGGAGAGCTGTTTTGGCCGTTGAACCGCCACCGTGAGGAGCTCCAGGAAGCGGCCATGGCGGATTCCGGCATCCTCAAGATGTTTGTTGATATTTATACTCATAAGAATCTTGTTGAAACGATGACAAAGGTAATGGAGAAAAGCAAGTCAATAGTAATTACAGGTCATTCACTTGGAGGAGCAGCAGCCACTCTATGTACACTTTGGCTGCTTTCTTTCTTCCATACCAAAACCCATCATCACCCAATTCTATGCATCACCTTTGGCTCCCCATTGATAGGCAATGAGTCACTTTCTAGAGCCATTCAAAGAGAACGATGGTGCGGCAAGTTTTGCCATGTCGTCTCCAACTACGACATTATGCCAAGGCTTCTCTCTACTCCACTTTCCTCTCTCTCCCCTAAGCTCCATATCCTCTTTAGATATTGGCATTTGTCAATGGCCTCTCCACATTTTGGGAAGCTTGCTACCCAATTGACAGAAAGAGAGAAAGAAGAGCTCTTCCACGTCGTATTGGCTCACTCCAACTCGATATCCGACCTAGGAGAGGGATCGGTACGAGGTCAGTTTTGGCCATTTGGAAACTTCTTCTTTTGCTCGGAACACGGTGCAATTTGTTTGGATAATGCCATGTCAGTTCTTAAGATGCTCTATCTGATGCTCAAAACAAGTGCTCCAAACTTGAGCATTGAGGACCATCTCAACTATGGAGATCATGTGAGAAAAGTTGGAGTTCAATACATGGAGAGGAAGAACTTCAATTCATTGAGTCTTCCTAATTCAAGCTATGAAGCTGGGCTTGCTCTAGCATTGCAGTCAGCAGGAATACCTTTCCAAGATGAAGTTGCTCGAATGGCCGAACATAGCTTGAGAACAGCAAGCAGAATAGGACAAACACCAAACTTGAATGCGGCAAAACTAGCTATAAGCTTATCAAAGATTACACCTTACAGGGCTGAGATAGAATGGTACAAAGCCTCTTGCGAGGAGGCAGATAACCAATTGGGTTACTACGATTGCTTCAAGAAAGAAGATGCTTCGGTAAGACATGACCGAGTAAATATGAACAGGCACAAGCTTGCAACATTCTGGAACAGAGTGATCGACATGTGGGAGAACAATGAGCTTCCTCCAGATTTTAACACAAGGGCAAAGTGGGTCAATGCTTCACAATTCTACAAACTCCTGGTAGAGCCACTGGACATTGCAGAATATTACCGTCGTGATAACCACATTGTCCATGGTCATTATTTGAATAGCGGAAGAGAGAGAAGATATGAGATTTTTGACAAATGGTGGAGAGGAAGAGAAGTCACAGAGGAAGGGAACACTCAGAGGATGAAATATGCAAGCTTGACCCAAGACTCATGCTTTTGGGCAAGGTTGGAGGAAGCCAAAGACCTACTGGAAATCATTAAAAGAGAAGGAGATGTGAGAAAGTTGGCTCCAATATGGAAAAGTCTTGAAAACTTTGAAAGGTATGCCAGAGGATTGATTGAAAGAAAGGAGGTTTCTAAAGATGTCATAGCAAAGAATTCAAGCTACACTTTGTGGGCTCAAGAACTGAGGGCATTGAAGCTAAATATGTAA

Coding sequence (CDS)

ATGGAGTCCGAAGCTTCAACATTCGAGAGTTGTCATGTCATGGCTGCTCTTTTGGGTTCAACTCCTCTGCTTCTGCAGTCGTGGGAATTCTGCGCCGCCGCCAACGCCACCTCCCCAGAAAGTTTCACGACTGTGGTGATTGATGATGTGGCTTATGTTGCCTTCTCCGGCGTCCAAGTTCTTGGTAGATGCGGCGGGGGAAGAGAGTTGGTGGCGTTGGATGGAGAGGGAGTTGAGGGAGAGCTGTTTTGGCCGTTGAACCGCCACCGTGAGGAGCTCCAGGAAGCGGCCATGGCGGATTCCGGCATCCTCAAGATGTTTGTTGATATTTATACTCATAAGAATCTTGTTGAAACGATGACAAAGGTAATGGAGAAAAGCAAGTCAATAGTAATTACAGGTCATTCACTTGGAGGAGCAGCAGCCACTCTATGTACACTTTGGCTGCTTTCTTTCTTCCATACCAAAACCCATCATCACCCAATTCTATGCATCACCTTTGGCTCCCCATTGATAGGCAATGAGTCACTTTCTAGAGCCATTCAAAGAGAACGATGGTGCGGCAAGTTTTGCCATGTCGTCTCCAACTACGACATTATGCCAAGGCTTCTCTCTACTCCACTTTCCTCTCTCTCCCCTAAGCTCCATATCCTCTTTAGATATTGGCATTTGTCAATGGCCTCTCCACATTTTGGGAAGCTTGCTACCCAATTGACAGAAAGAGAGAAAGAAGAGCTCTTCCACGTCGTATTGGCTCACTCCAACTCGATATCCGACCTAGGAGAGGGATCGGTACGAGGTCAGTTTTGGCCATTTGGAAACTTCTTCTTTTGCTCGGAACACGGTGCAATTTGTTTGGATAATGCCATGTCAGTTCTTAAGATGCTCTATCTGATGCTCAAAACAAGTGCTCCAAACTTGAGCATTGAGGACCATCTCAACTATGGAGATCATGTGAGAAAAGTTGGAGTTCAATACATGGAGAGGAAGAACTTCAATTCATTGAGTCTTCCTAATTCAAGCTATGAAGCTGGGCTTGCTCTAGCATTGCAGTCAGCAGGAATACCTTTCCAAGATGAAGTTGCTCGAATGGCCGAACATAGCTTGAGAACAGCAAGCAGAATAGGACAAACACCAAACTTGAATGCGGCAAAACTAGCTATAAGCTTATCAAAGATTACACCTTACAGGGCTGAGATAGAATGGTACAAAGCCTCTTGCGAGGAGGCAGATAACCAATTGGGTTACTACGATTGCTTCAAGAAAGAAGATGCTTCGGTAAGACATGACCGAGTAAATATGAACAGGCACAAGCTTGCAACATTCTGGAACAGAGTGATCGACATGTGGGAGAACAATGAGCTTCCTCCAGATTTTAACACAAGGGCAAAGTGGGTCAATGCTTCACAATTCTACAAACTCCTGGTAGAGCCACTGGACATTGCAGAATATTACCGTCGTGATAACCACATTGTCCATGGTCATTATTTGAATAGCGGAAGAGAGAGAAGATATGAGATTTTTGACAAATGGTGGAGAGGAAGAGAAGTCACAGAGGAAGGGAACACTCAGAGGATGAAATATGCAAGCTTGACCCAAGACTCATGCTTTTGGGCAAGGTTGGAGGAAGCCAAAGACCTACTGGAAATCATTAAAAGAGAAGGAGATGTGAGAAAGTTGGCTCCAATATGGAAAAGTCTTGAAAACTTTGAAAGGTATGCCAGAGGATTGATTGAAAGAAAGGAGGTTTCTAAAGATGTCATAGCAAAGAATTCAAGCTACACTTTGTGGGCTCAAGAACTGAGGGCATTGAAGCTAAATATGTAA

Protein sequence

MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQVLGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTEREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWARLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQELRALKLNM
Homology
BLAST of PI0016141 vs. ExPASy Swiss-Prot
Match: Q9S745 (Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.2e-104
Identity = 230/606 (37.95%), Postives = 333/606 (54.95%), Query Frame = 0

Query: 8   FESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVA---YVAFSGVQVLGRC 67
           FE+  + A+++ STPL   SW  C  AN        ++ I D+A   YVA   V ++   
Sbjct: 6   FETSELQASVMISTPLFTDSWSSCNTANCNG-----SIKIHDIAGITYVAIPAVSMI--- 65

Query: 68  GGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNL-VETMTKV 127
               +L  L G  V G++ +P     E L    M D+ ILK+F+ +   + L +E + K 
Sbjct: 66  ----QLGNLVGLPVTGDVLFPGLSSDEPL---PMVDAAILKLFLQLKIKEGLELELLGK- 125

Query: 128 MEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRAIQR 187
               K +VITGHS GGA A    LWLLS   +      + CITFGSPL+GN+SLS +I R
Sbjct: 126 ----KLVVITGHSTGGALAAFTALWLLS--QSSPPSFRVFCITFGSPLLGNQSLSTSISR 185

Query: 188 ERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTEREK 247
            R    FCHVVS +D++PR                                         
Sbjct: 186 SRLAHNFCHVVSIHDLVPR----------------------------------------- 245

Query: 248 EELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTS 307
                               S   QFWPFG + FCS+ G +CLDNA SV ++++ +L T+
Sbjct: 246 --------------------SSNEQFWPFGTYLFCSDKGGVCLDNAGSV-RLMFNILNTT 305

Query: 308 APNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVAR 367
           A   + E+H  YG +V  +   +++ ++F   S+P++SY+AG+ALA+++ G    D    
Sbjct: 306 ATQ-NTEEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFSNDDTSGV 365

Query: 368 MAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 427
           + +  + TA+RI + P L +A+LA  L+ + P R EI+WYK  C+ ++ QLGYYD FK+ 
Sbjct: 366 LVKECIETATRIVRAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDFFKRY 425

Query: 428 DASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 487
               R  +VNM+R +LA FW+ VI M E NELP DF+   KW+ ASQFY+LL EPLDIA 
Sbjct: 426 SLK-RDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLDIAN 485

Query: 488 YYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWARLEE 547
           +Y+  +    GHYL   R +RYE+ DKW +G +V EE    R +YAS TQD+CFWA+LE+
Sbjct: 486 FYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE--CVRSRYASTTQDTCFWAKLEQ 523

Query: 548 AKDLLEIIKRE-GDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQELR 607
           AK+ L+  ++E  D ++ + + + +  FE YA  L+ +KEVS DV AKNSSY++W   L+
Sbjct: 546 AKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLK 523

Query: 608 ALKLNM 609
             K  M
Sbjct: 606 EFKCKM 523

BLAST of PI0016141 vs. ExPASy Swiss-Prot
Match: Q9SU72 (Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.8e-36
Identity = 134/506 (26.48%), Postives = 223/506 (44.07%), Query Frame = 0

Query: 95  EAAMADSGILKMFVDIYTHKNLVETMTKVMEKS-KSIVITGHSLGGAAATLCTLWLLS-- 154
           + A  +   LK    I   +   +   ++  +S K IV TGHS GGA A L T+W L   
Sbjct: 81  DVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKY 140

Query: 155 FFHTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSL 214
           F      +    C+TFG+PL+G+   S A+ RE+W   F + VS +DI+PR++    +S+
Sbjct: 141 FIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASV 200

Query: 215 SPKL-HILFRYWHLSMASPHFGKLATQLTEREKEELFHVVLAHSNSISDLGEGSVRGQ-- 274
              L H+      L+   P   K + Q +E+   E +  V+  ++++++     + G   
Sbjct: 201 EETLPHV------LAQLDPR--KSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAE 260

Query: 275 --------------FWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTS-------APNL 334
                         + P G F F +E   + ++N+ ++L+ML+   + S        P  
Sbjct: 261 AFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFR 320

Query: 335 SIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVARMAEH 394
           SI DH +Y + V+ +G     +K FN L   NS       L + + G  +    A + E 
Sbjct: 321 SIRDHHSYEELVQSMG-----KKLFNHLDGENSIESTLNDLGVSTRGRQYVQ--AALEEE 380

Query: 395 SLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEW----YKASCEEADNQLGYYDCFKKE 454
             R  ++          K  I + +   +  ++ W    YK  C+   N  GYYD FK  
Sbjct: 381 KKRVENQ----------KKIIQVIEQERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVS 440

Query: 455 DASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 514
           +      + N+ R +LA  ++ V+ + +  +LP +F     W+  +  Y+ LVEPLDIA 
Sbjct: 441 NEE-NDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIAN 500

Query: 515 YYRRDNHIVHGHYLNSGR-------ERRYEIF----------DKWWR-------GREVTE 546
           Y+R   +   G Y+  GR       +R YE +          D +W        G ++ E
Sbjct: 501 YHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEE 556

BLAST of PI0016141 vs. ExPASy Swiss-Prot
Match: Q9SU71 (Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 3.4e-35
Identity = 130/521 (24.95%), Postives = 229/521 (43.95%), Query Frame = 0

Query: 119 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLL-SFFHTKTHHHP-ILCITFGSPLIGNES 178
           ++   +++ + +V TGHS GGA A L T+W L ++F    +  P   C+TFG+PL+G+  
Sbjct: 106 SVQSAVDRKQQVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYI 165

Query: 179 LSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPH-FGKLA 238
              A+ RE W   F + V+ +DI+PR++    +++   L  +      + A  H   ++ 
Sbjct: 166 FKHALGRENWSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVI 225

Query: 239 TQLTEREKEELFHVVLAHSNSISDLGEGSVR--------GQFWPFGNFFFCSEHGAICLD 298
           T+   R   + + V       +   GE  +           + P G F F ++   + ++
Sbjct: 226 TEFYTRVMRDTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVFSTQKRLVVVN 285

Query: 299 NAMSVLKMLYLMLKTS-------APNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNS 358
           N+ ++L+ML+   +++        P LSI DH  Y + V+ +G++ +   N   L  P  
Sbjct: 286 NSDAILQMLFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLL---NHLDLHNPLL 345

Query: 359 SYEAGLALALQSAGIPFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEI 418
             E  +  AL   G+  +   AR   H+   A +       N  K+     +I      I
Sbjct: 346 DGENSIGSALDDLGMSTR---ARQCIHAALEAEKQRVE---NQKKIETKRDQIVERLTWI 405

Query: 419 -EWYKASCEEADNQLGYYDCFKKEDASVRHD-RVNMNRHKLATFWNRVIDMWENNELPPD 478
            E YK  C+   N  GYYD FK  D++  +D + N+ R +LA  ++ V+ + +  +LP  
Sbjct: 406 VEVYKPKCQAHKN--GYYDSFK--DSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDG 465

Query: 479 FNTRAKWVNASQFYKLLVEPLDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVT 538
           F     W+N +  Y+ L+EPLDI+ Y+ +  +   G Y+  GR  RY+   + +    + 
Sbjct: 466 FEGSRGWINLATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILK 525

Query: 539 EEGNTQRMKYAS--------LTQD-------------SCFWARLEEAKDLLEIIKREGDV 598
             G   +  + S        L QD             SCFWA +EE K            
Sbjct: 526 PTGMIAKDVFWSKVNGLNLGLQQDIQEILKNSGSECGSCFWAEVEELKG----------- 585

BLAST of PI0016141 vs. ExPASy Swiss-Prot
Match: Q9XF23 (Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-34
Identity = 130/506 (25.69%), Postives = 219/506 (43.28%), Query Frame = 0

Query: 95  EAAMADSGILKMFVDIYTHKNLVETMTKVMEKS-KSIVITGHSLGGAAATLCTLWLLS-- 154
           + A  +   LK    +   +   +   ++  +S K IV TGHS GGA A L T+W L   
Sbjct: 81  DVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKY 140

Query: 155 FFHTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSL 214
           F      +    C+TFG+PL+G+   S A+ RE+W   F + V+ +DI+PR+     +S+
Sbjct: 141 FIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVTRFDIVPRITLARKASV 200

Query: 215 SPKL-HILFRYWHLSMASPHFGKLATQLTEREKEELFHVVLAHSNSISDLGEGSVRGQ-- 274
              L H+      L+   P     + Q +E+   E +  V+  ++++++     + G   
Sbjct: 201 EETLPHV------LAQLDPR--NSSVQESEQRITEFYTSVMRDTSTVANQAVCELTGSAE 260

Query: 275 --------------FWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTS-------APNL 334
                         + P G F F +E   + ++N+ ++L+ML+   + S        P  
Sbjct: 261 AILETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTCQASDEQEWSLIPFR 320

Query: 335 SIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVARMAEH 394
           SI DH +Y + V+ +G+     K FN L   NS   +   L + + G  +    A + E 
Sbjct: 321 SIRDHHSYEELVQSMGM-----KLFNHLDGENSIESSLNDLGVSTRGRQYVQ--AALEEE 380

Query: 395 SLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEW----YKASCEEADNQLGYYDCFKKE 454
             R  ++          K  I + +   +  ++ W    YK  C+   N  GYYD FK  
Sbjct: 381 KKRVENQ----------KKIIQVIQQERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVS 440

Query: 455 DASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 514
           +      + N+ R +LA  ++ V+ + +  +LP +F     W+  +  Y+ LVEPLDIA 
Sbjct: 441 NEE-NDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIAN 500

Query: 515 YYRRDNHIVHGHYLNSGRERRYEIF-----------------DKWWR-------GREVTE 546
           Y+R   +   G Y+  GR  RY                    D +W        G ++ E
Sbjct: 501 YHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEE 556

BLAST of PI0016141 vs. ExPASy Swiss-Prot
Match: Q4F883 (Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SAG101 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.3e-31
Identity = 127/500 (25.40%), Postives = 204/500 (40.80%), Query Frame = 0

Query: 116 LVETMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNE 175
           L   + + ++  K ++ITG +LGG+ A+L TLWLL      T   P LCITFGSPLIG+ 
Sbjct: 126 LKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIE-PTLKRP-LCITFGSPLIGDA 185

Query: 176 SLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLA 235
           SL + ++       F HVVS                                        
Sbjct: 186 SLQQILENSVRNSCFLHVVS---------------------------------------- 245

Query: 236 TQLTEREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKM 295
                R K + F                       PFG F  C + G +C+++ ++V ++
Sbjct: 246 --AQTRIKMDFFK----------------------PFGTFLICFDSGCVCIEDHVAVTEL 305

Query: 296 LYLMLKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGI 355
           L  +  +   + S                Q + R + + LSL +S               
Sbjct: 306 LNGVHDSGLVDYS----------------QVLNRLDQSMLSLADSRL------------- 365

Query: 356 PFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLG 415
                   + E  ++   +  +  NL    +   L+ +    A IEWYK  C+E   ++G
Sbjct: 366 --------IPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEV--KIG 425

Query: 416 YYDCFKKEDA--SVRHDRVNMNRHK--LATFWNRVIDMWENNELPPDFNTRAKWVNASQF 475
           YYD FK + A  S   D    N HK  L  FW  V++  E          + +++ +   
Sbjct: 426 YYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNN 485

Query: 476 YKLLVEPLDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYAS- 535
           Y+ ++EPLDIAEYY          Y  +GR   Y + +KW+    +  E    + +  S 
Sbjct: 486 YRRMIEPLDIAEYYLEGRK----EYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSD 514

Query: 536 -LTQDSCFWARLEEA----KDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSK 595
            LT DSCFWA +E++      L   +    DVR++  + + L  FE Y   +I ++EVS 
Sbjct: 546 LLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREV--LTRKLVEFEGYVWEIITKREVSP 514

Query: 596 DVIAKNSSYTLWAQELRALK 606
           ++  + SS+  W +E + +K
Sbjct: 606 EIFLEESSFMKWWKEYKKIK 514

BLAST of PI0016141 vs. ExPASy TrEMBL
Match: A0A0A0L3S4 (PAD4 OS=Cucumis sativus OX=3659 GN=Csa_4G496760 PE=4 SV=1)

HSP 1 Score: 1176.8 bits (3043), Expect = 0.0e+00
Identity = 576/609 (94.58%), Postives = 589/609 (96.72%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANA SPESFTTVVIDDVAYV FSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60

Query: 61  LGRC-GGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVET 120
           L RC GGGRELVALDGEGVE ELFWPLNRHREELQE AMADSGILKMFVDIYTHKNLVET
Sbjct: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120

Query: 121 MTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSR 180
           +TKVME+SKSIVITGHSLGGAAATLCTLWLLSF HTKTHHHPILCITFGSPLIGNESLSR
Sbjct: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180

Query: 181 AIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           AIQRERWCGKFCHVVSN+DIMPRLLSTPLSSLSPKLHIL RYWHLSMASP FGKLATQLT
Sbjct: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           EREKEELFH+VLAHSN ISDLGEG+V+ QFWPFGNFFFCSEHGAICLDNA+SVLKMLYLM
Sbjct: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTSAPNLSIEDHLNYG HV+KVGVQYMERKNFNS   PNSSYEAGLALALQSAGIPFQD
Sbjct: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVA++AEH LRTASRIGQTPN+NAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC
Sbjct: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FKKEDASVRHDRVNMNRHKLATFWNRVI+MWENNELPPDFNTRAKWVNASQFYKLLVEPL
Sbjct: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY RD HIVHGHYL  GRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA
Sbjct: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKDLLEIIKR+GDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ
Sbjct: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 609

BLAST of PI0016141 vs. ExPASy TrEMBL
Match: A0A1S3B5S6 (lipase-like PAD4 OS=Cucumis melo OX=3656 GN=LOC103486086 PE=4 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 574/610 (94.10%), Postives = 587/610 (96.23%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANA SPESFTT+VIDDVAYVAFSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTLVIDDVAYVAFSGVQV 60

Query: 61  LGRCGGG--RELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVE 120
           L  CGGG  RELVALDGEGVEGELFWPL RHREELQE AMADSGILKMFVDIY HKNLVE
Sbjct: 61  LPGCGGGRVRELVALDGEGVEGELFWPLKRHREELQEPAMADSGILKMFVDIYAHKNLVE 120

Query: 121 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS 180
           TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS
Sbjct: 121 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS 180

Query: 181 RAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQL 240
           RAIQRERWCGKFCHVVSN+DIMPRLLSTPL SLSPKLHI+FRYWHLSMASP FGKLATQL
Sbjct: 181 RAIQRERWCGKFCHVVSNHDIMPRLLSTPLPSLSPKLHIIFRYWHLSMASPQFGKLATQL 240

Query: 241 TEREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYL 300
           TEREKEELF++VLAHSN+I DLGEGSV+ QFWPFGNFFFCSEHGAICLDNA+SVLKML L
Sbjct: 241 TEREKEELFYIVLAHSNTIFDLGEGSVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLDL 300

Query: 301 MLKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQ 360
           MLKTSAPNLSIEDHLNYGDHV+KVGVQYMERKN NSL LPNSSYEAGLALAL+SAGIPFQ
Sbjct: 301 MLKTSAPNLSIEDHLNYGDHVKKVGVQYMERKNLNSLCLPNSSYEAGLALALESAGIPFQ 360

Query: 361 DEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYD 420
           DEVA MAEHSLRTASRIGQTPN+NAAKLAISLSKITPYRAEIEWYK SC+EADNQLGYYD
Sbjct: 361 DEVAHMAEHSLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKTSCDEADNQLGYYD 420

Query: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEP 480
           CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFN RAKWVNASQFYKLLVEP
Sbjct: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNMRAKWVNASQFYKLLVEP 480

Query: 481 LDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFW 540
           LDIAEYYRRD HIVHGHYL  GRERRYEIFDKWWRGREVTEEGNT RMKYASLTQDSCFW
Sbjct: 481 LDIAEYYRRDTHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTPRMKYASLTQDSCFW 540

Query: 541 ARLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWA 600
           ARLEEAKDLLEIIKR GDVRKLAPIWKSLENFERYARGLIERKEVS+DVIAKNSSYTLWA
Sbjct: 541 ARLEEAKDLLEIIKR-GDVRKLAPIWKSLENFERYARGLIERKEVSEDVIAKNSSYTLWA 600

Query: 601 QELRALKLNM 609
           QELRALKLNM
Sbjct: 601 QELRALKLNM 609

BLAST of PI0016141 vs. ExPASy TrEMBL
Match: A0A6J1HT58 (lipase-like PAD4 OS=Cucurbita maxima OX=3661 GN=LOC111465963 PE=4 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.7e-298
Identity = 499/609 (81.94%), Postives = 555/609 (91.13%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESE+STFESC +MAALLGS+PLLLQSW+ CAAANA +PE+F+  VI DVA+VAFS VQ+
Sbjct: 1   MESESSTFESCEIMAALLGSSPLLLQSWKLCAAANAAAPETFSVEVIGDVAFVAFSAVQI 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L   GGGRELVAL+G+    ELFWPLNRHREEL+  AMADSGILK+F+ I+TH+NL+E M
Sbjct: 61  LPGSGGGRELVALEGDA--EELFWPLNRHREELRAPAMADSGILKIFMSIFTHQNLLEKM 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           TKVMEKSKSIVITGHSLGGAAA+LCTLWLLS FH+KT HHP+LCITFGSPLIGNESLSRA
Sbjct: 121 TKVMEKSKSIVITGHSLGGAAASLCTLWLLSLFHSKTTHHPLLCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPL-SSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           I RERWCG FCHVVSN+DIMPRLLSTPL SSLS +LH+L RYWHLS+ASP FGKLATQL+
Sbjct: 181 ILRERWCGNFCHVVSNHDIMPRLLSTPLSSSLSSQLHVLLRYWHLSVASPQFGKLATQLS 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           E+EK+ELF VVLAHSN+IS+  EGS++ QFWPFGNFFFCS +GAICLDNA+SVLKMLYL+
Sbjct: 241 EKEKDELFRVVLAHSNTISNSEEGSIQSQFWPFGNFFFCSGNGAICLDNAISVLKMLYLL 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTS PN+SIEDHLNYGD+V+KVGVQ+MERKNF S  LPNSSYEAGLALALQS+GIPFQD
Sbjct: 301 LKTSVPNISIEDHLNYGDYVKKVGVQHMERKNFASERLPNSSYEAGLALALQSSGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAE+SLRTA R+GQTPNLNAAKLA+SLSKITPYRAEIEWYKASC+EA +QLGYYDC
Sbjct: 361 EVARMAENSLRTARRMGQTPNLNAAKLAVSLSKITPYRAEIEWYKASCDEAGDQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK+ + SV+H RVNMNRHKLATFWNRVIDMWENNELPPDFN RAKW+NAS FYKLLVEPL
Sbjct: 421 FKQVNTSVKHARVNMNRHKLATFWNRVIDMWENNELPPDFNLRAKWINASHFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY R  HI HGHYL  GR+RRYEIFDKWWRG +  ++GNTQR KYA LTQDSCFWA
Sbjct: 481 DIAEYYCRGKHITHGHYLKHGRDRRYEIFDKWWRGSDAADKGNTQRTKYAGLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAK+LLEIIK EGDVRKLAP+WKSLENFERYARGL+ERKEVSKDV+AKNSSYTLWAQ
Sbjct: 541 RLEEAKELLEIIKCEGDVRKLAPVWKSLENFERYARGLVERKEVSKDVVAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 607

BLAST of PI0016141 vs. ExPASy TrEMBL
Match: A0A6J1EHH0 (lipase-like PAD4 OS=Cucurbita moschata OX=3662 GN=LOC111432561 PE=4 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.7e-296
Identity = 498/609 (81.77%), Postives = 555/609 (91.13%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESE+STFESC +MAALLGS+PLLLQSW  CAAANA +PE+F+  VI DVA+VAFS VQ+
Sbjct: 1   MESESSTFESCEIMAALLGSSPLLLQSWTLCAAANAAAPETFSVEVIGDVAFVAFSAVQI 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L   GGGRELVAL+G+ VE ELFWPLNRHREEL+  AMADSGILK+F+ I+TH+NL E M
Sbjct: 61  LPGSGGGRELVALEGD-VE-ELFWPLNRHREELRAPAMADSGILKIFMSIFTHQNLSEKM 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           TKVMEKSKSIVITGHSLGGAAA+LCTLWLLS FH+KT HHP+LCITFGSPLIGNESLSRA
Sbjct: 121 TKVMEKSKSIVITGHSLGGAAASLCTLWLLSLFHSKTTHHPLLCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPL-SSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           I RERWCG FCHVVSN+DIMPRLLSTPL SSLS +LH+L RYWHLS+AS  FGKLATQL+
Sbjct: 181 ILRERWCGNFCHVVSNHDIMPRLLSTPLSSSLSSQLHVLLRYWHLSLASLQFGKLATQLS 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           ++EK+ELF VVLAHSN+IS+  E S+R QFWPFGNFFFCSE+GAICLDNA+SVLKMLYL+
Sbjct: 241 DKEKDELFRVVLAHSNTISNSEEESLRSQFWPFGNFFFCSENGAICLDNAISVLKMLYLL 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTS PN+SIEDHLNYGD+V+KVGVQ+MERKNF+S  LPNSSYEAGLALALQS+GIPFQD
Sbjct: 301 LKTSVPNMSIEDHLNYGDYVKKVGVQHMERKNFSSERLPNSSYEAGLALALQSSGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAE+SLRTA R+GQTPNLNAAKLA+SLSKITPYRAEIEWYKASC+EA +QLGYYDC
Sbjct: 361 EVARMAENSLRTARRMGQTPNLNAAKLAVSLSKITPYRAEIEWYKASCDEAGDQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK+ + SV+H RVNMNRHKLATFWNRVI+MWENNELPPDFN RAKW+NAS FYKLLVEPL
Sbjct: 421 FKQVNTSVKHARVNMNRHKLATFWNRVINMWENNELPPDFNLRAKWINASHFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY R  HI HGHYL  GR+RRYEIFDKWWRG +  ++GNTQR KYA LTQDSCFWA
Sbjct: 481 DIAEYYCRGKHIAHGHYLKHGRDRRYEIFDKWWRGSDAADKGNTQRTKYAGLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKD+LEIIK EGDVRKLAP+W+SLENFERYARGL+ERKEVSKDV+AKNSSYTLWAQ
Sbjct: 541 RLEEAKDMLEIIKCEGDVRKLAPVWRSLENFERYARGLVERKEVSKDVVAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 607

BLAST of PI0016141 vs. ExPASy TrEMBL
Match: A0A6J1D6Z0 (lipase-like PAD4 OS=Momordica charantia OX=3673 GN=LOC111017842 PE=4 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 1.2e-282
Identity = 484/611 (79.21%), Postives = 537/611 (87.89%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEAS FE+C +MAALLGS+PLL QSW  C   +AT+PE+FT  +I DVAYVAFS VQV
Sbjct: 1   MESEASMFETCEIMAALLGSSPLLSQSWSLC---SATAPETFTAELIGDVAYVAFSAVQV 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L    GGRELVAL+GE    ELF PLNRHREEL+   MADSG+L++F++I+TH+NL + M
Sbjct: 61  LPLARGGRELVALEGEA--EELFRPLNRHREELRPPPMADSGLLRIFLNIFTHQNLSQKM 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           T++M+KSKSIVITGHSLGGAAATLCTLWLLSFFH+KT H P+LCITFGSPLIGNESLSRA
Sbjct: 121 TRIMQKSKSIVITGHSLGGAAATLCTLWLLSFFHSKTTHQPLLCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTE 240
           I RERWCG FCHVVSN+DIMPRL STP   LSP+LH+L RYWHLSM SP FGKLATQL++
Sbjct: 181 ILRERWCGNFCHVVSNHDIMPRLFSTP---LSPQLHVLLRYWHLSMVSPQFGKLATQLSQ 240

Query: 241 REKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLML 300
           REK+ELF  VLAH + IS+ GEGS R QFWP GNFFFCSE+GAICLDNAMSV+KMLYLML
Sbjct: 241 REKDELFQAVLAHFDMISNSGEGSERSQFWPLGNFFFCSENGAICLDNAMSVMKMLYLML 300

Query: 301 KTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNS-LSLPNSSYEAGLALALQSAGIPFQD 360
           KTSAPN SIEDHLNYGD+V++VG+Q+MERK+F S   LP+SSYEAGLALA+QS+GIPFQ+
Sbjct: 301 KTSAPNFSIEDHLNYGDYVKEVGIQHMERKSFTSECLLPDSSYEAGLALAIQSSGIPFQE 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAE+SLRTA R+G  P L+ AKLAISLSKITPYRAEIEWYKASC+EADNQLGYYDC
Sbjct: 361 EVARMAENSLRTARRMGLKPTLSVAKLAISLSKITPYRAEIEWYKASCDEADNQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK+ DASVR  RVNMNRHKLATFWNRVID WENNELPPDFN RAKWVNAS  YKLLVEPL
Sbjct: 421 FKRVDASVRQARVNMNRHKLATFWNRVIDRWENNELPPDFNIRAKWVNASHSYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNT--QRMKYASLTQDSCF 540
           DIAEYYRR  H+VHGHYL  GRERRYEIFDKWWRG+E+ E+GNT  QRMKYA LTQDSCF
Sbjct: 481 DIAEYYRRGKHLVHGHYLKHGRERRYEIFDKWWRGKEIREQGNTQSQRMKYAGLTQDSCF 540

Query: 541 WARLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLW 600
           WARLEEA+DLLE IK EGDVRKLA IWKSLENFERYARGLIERKEVSKD +AKNSSYTLW
Sbjct: 541 WARLEEARDLLESIKCEGDVRKLALIWKSLENFERYARGLIERKEVSKDAVAKNSSYTLW 600

Query: 601 AQELRALKLNM 609
           AQELRALKLNM
Sbjct: 601 AQELRALKLNM 603

BLAST of PI0016141 vs. NCBI nr
Match: XP_011653897.1 (lipase-like PAD4 [Cucumis sativus] >KGN54786.1 hypothetical protein Csa_012136 [Cucumis sativus])

HSP 1 Score: 1176.8 bits (3043), Expect = 0.0e+00
Identity = 576/609 (94.58%), Postives = 589/609 (96.72%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANA SPESFTTVVIDDVAYV FSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60

Query: 61  LGRC-GGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVET 120
           L RC GGGRELVALDGEGVE ELFWPLNRHREELQE AMADSGILKMFVDIYTHKNLVET
Sbjct: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120

Query: 121 MTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSR 180
           +TKVME+SKSIVITGHSLGGAAATLCTLWLLSF HTKTHHHPILCITFGSPLIGNESLSR
Sbjct: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180

Query: 181 AIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           AIQRERWCGKFCHVVSN+DIMPRLLSTPLSSLSPKLHIL RYWHLSMASP FGKLATQLT
Sbjct: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           EREKEELFH+VLAHSN ISDLGEG+V+ QFWPFGNFFFCSEHGAICLDNA+SVLKMLYLM
Sbjct: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTSAPNLSIEDHLNYG HV+KVGVQYMERKNFNS   PNSSYEAGLALALQSAGIPFQD
Sbjct: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVA++AEH LRTASRIGQTPN+NAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC
Sbjct: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FKKEDASVRHDRVNMNRHKLATFWNRVI+MWENNELPPDFNTRAKWVNASQFYKLLVEPL
Sbjct: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY RD HIVHGHYL  GRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA
Sbjct: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKDLLEIIKR+GDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ
Sbjct: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 609

BLAST of PI0016141 vs. NCBI nr
Match: XP_008442139.1 (PREDICTED: lipase-like PAD4 [Cucumis melo])

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 574/610 (94.10%), Postives = 587/610 (96.23%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANA SPESFTT+VIDDVAYVAFSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTLVIDDVAYVAFSGVQV 60

Query: 61  LGRCGGG--RELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVE 120
           L  CGGG  RELVALDGEGVEGELFWPL RHREELQE AMADSGILKMFVDIY HKNLVE
Sbjct: 61  LPGCGGGRVRELVALDGEGVEGELFWPLKRHREELQEPAMADSGILKMFVDIYAHKNLVE 120

Query: 121 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS 180
           TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS
Sbjct: 121 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS 180

Query: 181 RAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQL 240
           RAIQRERWCGKFCHVVSN+DIMPRLLSTPL SLSPKLHI+FRYWHLSMASP FGKLATQL
Sbjct: 181 RAIQRERWCGKFCHVVSNHDIMPRLLSTPLPSLSPKLHIIFRYWHLSMASPQFGKLATQL 240

Query: 241 TEREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYL 300
           TEREKEELF++VLAHSN+I DLGEGSV+ QFWPFGNFFFCSEHGAICLDNA+SVLKML L
Sbjct: 241 TEREKEELFYIVLAHSNTIFDLGEGSVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLDL 300

Query: 301 MLKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQ 360
           MLKTSAPNLSIEDHLNYGDHV+KVGVQYMERKN NSL LPNSSYEAGLALAL+SAGIPFQ
Sbjct: 301 MLKTSAPNLSIEDHLNYGDHVKKVGVQYMERKNLNSLCLPNSSYEAGLALALESAGIPFQ 360

Query: 361 DEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYD 420
           DEVA MAEHSLRTASRIGQTPN+NAAKLAISLSKITPYRAEIEWYK SC+EADNQLGYYD
Sbjct: 361 DEVAHMAEHSLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKTSCDEADNQLGYYD 420

Query: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEP 480
           CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFN RAKWVNASQFYKLLVEP
Sbjct: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNMRAKWVNASQFYKLLVEP 480

Query: 481 LDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFW 540
           LDIAEYYRRD HIVHGHYL  GRERRYEIFDKWWRGREVTEEGNT RMKYASLTQDSCFW
Sbjct: 481 LDIAEYYRRDTHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTPRMKYASLTQDSCFW 540

Query: 541 ARLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWA 600
           ARLEEAKDLLEIIKR GDVRKLAPIWKSLENFERYARGLIERKEVS+DVIAKNSSYTLWA
Sbjct: 541 ARLEEAKDLLEIIKR-GDVRKLAPIWKSLENFERYARGLIERKEVSEDVIAKNSSYTLWA 600

Query: 601 QELRALKLNM 609
           QELRALKLNM
Sbjct: 601 QELRALKLNM 609

BLAST of PI0016141 vs. NCBI nr
Match: XP_038881104.1 (lipase-like PAD4 [Benincasa hispida])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 542/609 (89.00%), Postives = 570/609 (93.60%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESEASTFESC +MAALLGS+PLLLQSW  CAAAN+ +PESFT  VI DVAY+AFSGVQV
Sbjct: 1   MESEASTFESCEIMAALLGSSPLLLQSWRLCAAANSAAPESFTAEVIGDVAYLAFSGVQV 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L +CG GRELVALD  GVE E+F PLNRHR+EL+E AMADSGILKMFVDIYTH+NL+E  
Sbjct: 61  LPKCGDGRELVALDAGGVE-EMFLPLNRHRQELREPAMADSGILKMFVDIYTHQNLLEKT 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           T+V+EKSKSIVITGHSLGGAAATLCTLWLLSFFH KT HHPILCITFGSPLIGNESLSRA
Sbjct: 121 TRVLEKSKSIVITGHSLGGAAATLCTLWLLSFFHPKTIHHPILCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTE 240
           IQRERWCGKFCHV+SN+DIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTE
Sbjct: 181 IQRERWCGKFCHVISNHDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTE 240

Query: 241 REKEELFHVVLAHSNSISDLG-EGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           REK+ELF VVLAHSN IS+ G EGSV+ Q WPFGNFFFCS+HGAICLDNA+SVLKML LM
Sbjct: 241 REKDELFQVVLAHSNMISNPGLEGSVQSQLWPFGNFFFCSKHGAICLDNAISVLKMLDLM 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTSAPNLSIEDHLNYGDHV+KVGVQY+ERK+F S  LPNSSYEAGLALALQSAGIPFQD
Sbjct: 301 LKTSAPNLSIEDHLNYGDHVKKVGVQYIERKSFTSECLPNSSYEAGLALALQSAGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAEHSLRTA RIGQTPNLNAAKLAISLSKITPYRAEIEWYK SC+EAD+QLGYYDC
Sbjct: 361 EVARMAEHSLRTARRIGQTPNLNAAKLAISLSKITPYRAEIEWYKVSCDEADDQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FKK DASVRH RVNMNRHKLATFWNRVIDMWE NELPPDFNTRAKWVNAS FYKLLVEPL
Sbjct: 421 FKKVDASVRHARVNMNRHKLATFWNRVIDMWEYNELPPDFNTRAKWVNASHFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYYRRD HIVHGHYL  GRERRYEIFDKWW+GREVTEEGNTQRMKYASLTQDSCFWA
Sbjct: 481 DIAEYYRRDKHIVHGHYLKYGRERRYEIFDKWWKGREVTEEGNTQRMKYASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKDLLE IK EGDVRKLAPIWKSL+NFERYARGLIERKEVSKDV+AKNSSYTLWAQ
Sbjct: 541 RLEEAKDLLERIKCEGDVRKLAPIWKSLDNFERYARGLIERKEVSKDVVAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 608

BLAST of PI0016141 vs. NCBI nr
Match: XP_022966244.1 (lipase-like PAD4 [Cucurbita maxima])

HSP 1 Score: 1033.1 bits (2670), Expect = 9.6e-298
Identity = 499/609 (81.94%), Postives = 555/609 (91.13%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESE+STFESC +MAALLGS+PLLLQSW+ CAAANA +PE+F+  VI DVA+VAFS VQ+
Sbjct: 1   MESESSTFESCEIMAALLGSSPLLLQSWKLCAAANAAAPETFSVEVIGDVAFVAFSAVQI 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L   GGGRELVAL+G+    ELFWPLNRHREEL+  AMADSGILK+F+ I+TH+NL+E M
Sbjct: 61  LPGSGGGRELVALEGDA--EELFWPLNRHREELRAPAMADSGILKIFMSIFTHQNLLEKM 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           TKVMEKSKSIVITGHSLGGAAA+LCTLWLLS FH+KT HHP+LCITFGSPLIGNESLSRA
Sbjct: 121 TKVMEKSKSIVITGHSLGGAAASLCTLWLLSLFHSKTTHHPLLCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPL-SSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           I RERWCG FCHVVSN+DIMPRLLSTPL SSLS +LH+L RYWHLS+ASP FGKLATQL+
Sbjct: 181 ILRERWCGNFCHVVSNHDIMPRLLSTPLSSSLSSQLHVLLRYWHLSVASPQFGKLATQLS 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           E+EK+ELF VVLAHSN+IS+  EGS++ QFWPFGNFFFCS +GAICLDNA+SVLKMLYL+
Sbjct: 241 EKEKDELFRVVLAHSNTISNSEEGSIQSQFWPFGNFFFCSGNGAICLDNAISVLKMLYLL 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTS PN+SIEDHLNYGD+V+KVGVQ+MERKNF S  LPNSSYEAGLALALQS+GIPFQD
Sbjct: 301 LKTSVPNISIEDHLNYGDYVKKVGVQHMERKNFASERLPNSSYEAGLALALQSSGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAE+SLRTA R+GQTPNLNAAKLA+SLSKITPYRAEIEWYKASC+EA +QLGYYDC
Sbjct: 361 EVARMAENSLRTARRMGQTPNLNAAKLAVSLSKITPYRAEIEWYKASCDEAGDQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK+ + SV+H RVNMNRHKLATFWNRVIDMWENNELPPDFN RAKW+NAS FYKLLVEPL
Sbjct: 421 FKQVNTSVKHARVNMNRHKLATFWNRVIDMWENNELPPDFNLRAKWINASHFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY R  HI HGHYL  GR+RRYEIFDKWWRG +  ++GNTQR KYA LTQDSCFWA
Sbjct: 481 DIAEYYCRGKHITHGHYLKHGRDRRYEIFDKWWRGSDAADKGNTQRTKYAGLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAK+LLEIIK EGDVRKLAP+WKSLENFERYARGL+ERKEVSKDV+AKNSSYTLWAQ
Sbjct: 541 RLEEAKELLEIIKCEGDVRKLAPVWKSLENFERYARGLVERKEVSKDVVAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 607

BLAST of PI0016141 vs. NCBI nr
Match: XP_022925275.1 (lipase-like PAD4 [Cucurbita moschata])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.2e-295
Identity = 498/609 (81.77%), Postives = 555/609 (91.13%), Query Frame = 0

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVAYVAFSGVQV 60
           MESE+STFESC +MAALLGS+PLLLQSW  CAAANA +PE+F+  VI DVA+VAFS VQ+
Sbjct: 1   MESESSTFESCEIMAALLGSSPLLLQSWTLCAAANAAAPETFSVEVIGDVAFVAFSAVQI 60

Query: 61  LGRCGGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNLVETM 120
           L   GGGRELVAL+G+ VE ELFWPLNRHREEL+  AMADSGILK+F+ I+TH+NL E M
Sbjct: 61  LPGSGGGRELVALEGD-VE-ELFWPLNRHREELRAPAMADSGILKIFMSIFTHQNLSEKM 120

Query: 121 TKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRA 180
           TKVMEKSKSIVITGHSLGGAAA+LCTLWLLS FH+KT HHP+LCITFGSPLIGNESLSRA
Sbjct: 121 TKVMEKSKSIVITGHSLGGAAASLCTLWLLSLFHSKTTHHPLLCITFGSPLIGNESLSRA 180

Query: 181 IQRERWCGKFCHVVSNYDIMPRLLSTPL-SSLSPKLHILFRYWHLSMASPHFGKLATQLT 240
           I RERWCG FCHVVSN+DIMPRLLSTPL SSLS +LH+L RYWHLS+AS  FGKLATQL+
Sbjct: 181 ILRERWCGNFCHVVSNHDIMPRLLSTPLSSSLSSQLHVLLRYWHLSLASLQFGKLATQLS 240

Query: 241 EREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLM 300
           ++EK+ELF VVLAHSN+IS+  E S+R QFWPFGNFFFCSE+GAICLDNA+SVLKMLYL+
Sbjct: 241 DKEKDELFRVVLAHSNTISNSEEESLRSQFWPFGNFFFCSENGAICLDNAISVLKMLYLL 300

Query: 301 LKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQD 360
           LKTS PN+SIEDHLNYGD+V+KVGVQ+MERKNF+S  LPNSSYEAGLALALQS+GIPFQD
Sbjct: 301 LKTSVPNMSIEDHLNYGDYVKKVGVQHMERKNFSSERLPNSSYEAGLALALQSSGIPFQD 360

Query: 361 EVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVARMAE+SLRTA R+GQTPNLNAAKLA+SLSKITPYRAEIEWYKASC+EA +QLGYYDC
Sbjct: 361 EVARMAENSLRTARRMGQTPNLNAAKLAVSLSKITPYRAEIEWYKASCDEAGDQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK+ + SV+H RVNMNRHKLATFWNRVI+MWENNELPPDFN RAKW+NAS FYKLLVEPL
Sbjct: 421 FKQVNTSVKHARVNMNRHKLATFWNRVINMWENNELPPDFNLRAKWINASHFYKLLVEPL 480

Query: 481 DIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY R  HI HGHYL  GR+RRYEIFDKWWRG +  ++GNTQR KYA LTQDSCFWA
Sbjct: 481 DIAEYYCRGKHIAHGHYLKHGRDRRYEIFDKWWRGSDAADKGNTQRTKYAGLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKD+LEIIK EGDVRKLAP+W+SLENFERYARGL+ERKEVSKDV+AKNSSYTLWAQ
Sbjct: 541 RLEEAKDMLEIIKCEGDVRKLAPVWRSLENFERYARGLVERKEVSKDVVAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 609
           ELRALKLNM
Sbjct: 601 ELRALKLNM 607

BLAST of PI0016141 vs. TAIR 10
Match: AT3G52430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 8.3e-106
Identity = 230/606 (37.95%), Postives = 333/606 (54.95%), Query Frame = 0

Query: 8   FESCHVMAALLGSTPLLLQSWEFCAAANATSPESFTTVVIDDVA---YVAFSGVQVLGRC 67
           FE+  + A+++ STPL   SW  C  AN        ++ I D+A   YVA   V ++   
Sbjct: 6   FETSELQASVMISTPLFTDSWSSCNTANCNG-----SIKIHDIAGITYVAIPAVSMI--- 65

Query: 68  GGGRELVALDGEGVEGELFWPLNRHREELQEAAMADSGILKMFVDIYTHKNL-VETMTKV 127
               +L  L G  V G++ +P     E L    M D+ ILK+F+ +   + L +E + K 
Sbjct: 66  ----QLGNLVGLPVTGDVLFPGLSSDEPL---PMVDAAILKLFLQLKIKEGLELELLGK- 125

Query: 128 MEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLSRAIQR 187
               K +VITGHS GGA A    LWLLS   +      + CITFGSPL+GN+SLS +I R
Sbjct: 126 ----KLVVITGHSTGGALAAFTALWLLS--QSSPPSFRVFCITFGSPLLGNQSLSTSISR 185

Query: 188 ERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLATQLTEREK 247
            R    FCHVVS +D++PR                                         
Sbjct: 186 SRLAHNFCHVVSIHDLVPR----------------------------------------- 245

Query: 248 EELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTS 307
                               S   QFWPFG + FCS+ G +CLDNA SV ++++ +L T+
Sbjct: 246 --------------------SSNEQFWPFGTYLFCSDKGGVCLDNAGSV-RLMFNILNTT 305

Query: 308 APNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVAR 367
           A   + E+H  YG +V  +   +++ ++F   S+P++SY+AG+ALA+++ G    D    
Sbjct: 306 ATQ-NTEEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFSNDDTSGV 365

Query: 368 MAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 427
           + +  + TA+RI + P L +A+LA  L+ + P R EI+WYK  C+ ++ QLGYYD FK+ 
Sbjct: 366 LVKECIETATRIVRAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDFFKRY 425

Query: 428 DASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 487
               R  +VNM+R +LA FW+ VI M E NELP DF+   KW+ ASQFY+LL EPLDIA 
Sbjct: 426 SLK-RDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLDIAN 485

Query: 488 YYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWARLEE 547
           +Y+  +    GHYL   R +RYE+ DKW +G +V EE    R +YAS TQD+CFWA+LE+
Sbjct: 486 FYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE--CVRSRYASTTQDTCFWAKLEQ 523

Query: 548 AKDLLEIIKRE-GDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQELR 607
           AK+ L+  ++E  D ++ + + + +  FE YA  L+ +KEVS DV AKNSSY++W   L+
Sbjct: 546 AKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLK 523

Query: 608 ALKLNM 609
             K  M
Sbjct: 606 EFKCKM 523

BLAST of PI0016141 vs. TAIR 10
Match: AT3G48090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 4.8e-37
Identity = 134/506 (26.48%), Postives = 223/506 (44.07%), Query Frame = 0

Query: 95  EAAMADSGILKMFVDIYTHKNLVETMTKVMEKS-KSIVITGHSLGGAAATLCTLWLLS-- 154
           + A  +   LK    I   +   +   ++  +S K IV TGHS GGA A L T+W L   
Sbjct: 81  DVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKY 140

Query: 155 FFHTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSL 214
           F      +    C+TFG+PL+G+   S A+ RE+W   F + VS +DI+PR++    +S+
Sbjct: 141 FIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASV 200

Query: 215 SPKL-HILFRYWHLSMASPHFGKLATQLTEREKEELFHVVLAHSNSISDLGEGSVRGQ-- 274
              L H+      L+   P   K + Q +E+   E +  V+  ++++++     + G   
Sbjct: 201 EETLPHV------LAQLDPR--KSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAE 260

Query: 275 --------------FWPFGNFFFCSEHGAICLDNAMSVLKMLYLMLKTS-------APNL 334
                         + P G F F +E   + ++N+ ++L+ML+   + S        P  
Sbjct: 261 AFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFR 320

Query: 335 SIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGIPFQDEVARMAEH 394
           SI DH +Y + V+ +G     +K FN L   NS       L + + G  +    A + E 
Sbjct: 321 SIRDHHSYEELVQSMG-----KKLFNHLDGENSIESTLNDLGVSTRGRQYVQ--AALEEE 380

Query: 395 SLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEW----YKASCEEADNQLGYYDCFKKE 454
             R  ++          K  I + +   +  ++ W    YK  C+   N  GYYD FK  
Sbjct: 381 KKRVENQ----------KKIIQVIEQERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVS 440

Query: 455 DASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 514
           +      + N+ R +LA  ++ V+ + +  +LP +F     W+  +  Y+ LVEPLDIA 
Sbjct: 441 NEE-NDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIAN 500

Query: 515 YYRRDNHIVHGHYLNSGR-------ERRYEIF----------DKWWR-------GREVTE 546
           Y+R   +   G Y+  GR       +R YE +          D +W        G ++ E
Sbjct: 501 YHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEE 556

BLAST of PI0016141 vs. TAIR 10
Match: AT3G48090.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 1.1e-36
Identity = 129/472 (27.33%), Postives = 211/472 (44.70%), Query Frame = 0

Query: 128 KSIVITGHSLGGAAATLCTLWLLS--FFHTKTHHHPILCITFGSPLIGNESLSRAIQRER 187
           K IV TGHS GGA A L T+W L   F      +    C+TFG+PL+G+   S A+ RE+
Sbjct: 7   KQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREK 66

Query: 188 WCGKFCHVVSNYDIMPRLLSTPLSSLSPKL-HILFRYWHLSMASPHFGKLATQLTEREKE 247
           W   F + VS +DI+PR++    +S+   L H+      L+   P   K + Q +E+   
Sbjct: 67  WSRFFVNFVSRFDIVPRIMLARKASVEETLPHV------LAQLDPR--KSSVQESEQRIT 126

Query: 248 ELFHVVLAHSNSISDLGEGSVRGQ----------------FWPFGNFFFCSEHGAICLDN 307
           E +  V+  ++++++     + G                 + P G F F +E   + ++N
Sbjct: 127 EFYTRVMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNN 186

Query: 308 AMSVLKMLYLMLKTS-------APNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSS 367
           + ++L+ML+   + S        P  SI DH +Y + V+ +G     +K FN L   NS 
Sbjct: 187 SDAILQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMG-----KKLFNHLDGENSI 246

Query: 368 YEAGLALALQSAGIPFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIE 427
                 L + + G  +    A + E   R  ++          K  I + +   +  ++ 
Sbjct: 247 ESTLNDLGVSTRGRQYVQ--AALEEEKKRVENQ----------KKIIQVIEQERFLKKLA 306

Query: 428 W----YKASCEEADNQLGYYDCFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPP 487
           W    YK  C+   N  GYYD FK  +      + N+ R +LA  ++ V+ + +  +LP 
Sbjct: 307 WIEDEYKPKCQAHKN--GYYDSFKVSNEE-NDFKANVKRAELAGVFDEVLGLMKKCQLPD 366

Query: 488 DFNTRAKWVNASQFYKLLVEPLDIAEYYRRDNHIVHGHYLNSGR-------ERRYEIF-- 546
           +F     W+  +  Y+ LVEPLDIA Y+R   +   G Y+  GR       +R YE +  
Sbjct: 367 EFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYIL 426

BLAST of PI0016141 vs. TAIR 10
Match: AT3G48080.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 2.4e-36
Identity = 130/521 (24.95%), Postives = 229/521 (43.95%), Query Frame = 0

Query: 119 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLL-SFFHTKTHHHP-ILCITFGSPLIGNES 178
           ++   +++ + +V TGHS GGA A L T+W L ++F    +  P   C+TFG+PL+G+  
Sbjct: 106 SVQSAVDRKQQVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYI 165

Query: 179 LSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPH-FGKLA 238
              A+ RE W   F + V+ +DI+PR++    +++   L  +      + A  H   ++ 
Sbjct: 166 FKHALGRENWSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVI 225

Query: 239 TQLTEREKEELFHVVLAHSNSISDLGEGSVR--------GQFWPFGNFFFCSEHGAICLD 298
           T+   R   + + V       +   GE  +           + P G F F ++   + ++
Sbjct: 226 TEFYTRVMRDTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVFSTQKRLVVVN 285

Query: 299 NAMSVLKMLYLMLKTS-------APNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNS 358
           N+ ++L+ML+   +++        P LSI DH  Y + V+ +G++ +   N   L  P  
Sbjct: 286 NSDAILQMLFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLL---NHLDLHNPLL 345

Query: 359 SYEAGLALALQSAGIPFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEI 418
             E  +  AL   G+  +   AR   H+   A +       N  K+     +I      I
Sbjct: 346 DGENSIGSALDDLGMSTR---ARQCIHAALEAEKQRVE---NQKKIETKRDQIVERLTWI 405

Query: 419 -EWYKASCEEADNQLGYYDCFKKEDASVRHD-RVNMNRHKLATFWNRVIDMWENNELPPD 478
            E YK  C+   N  GYYD FK  D++  +D + N+ R +LA  ++ V+ + +  +LP  
Sbjct: 406 VEVYKPKCQAHKN--GYYDSFK--DSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDG 465

Query: 479 FNTRAKWVNASQFYKLLVEPLDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVT 538
           F     W+N +  Y+ L+EPLDI+ Y+ +  +   G Y+  GR  RY+   + +    + 
Sbjct: 466 FEGSRGWINLATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILK 525

Query: 539 EEGNTQRMKYAS--------LTQD-------------SCFWARLEEAKDLLEIIKREGDV 598
             G   +  + S        L QD             SCFWA +EE K            
Sbjct: 526 PTGMIAKDVFWSKVNGLNLGLQQDIQEILKNSGSECGSCFWAEVEELKG----------- 585

BLAST of PI0016141 vs. TAIR 10
Match: AT5G14930.2 (senescence-associated gene 101 )

HSP 1 Score: 139.4 bits (350), Expect = 9.4e-33
Identity = 127/500 (25.40%), Postives = 204/500 (40.80%), Query Frame = 0

Query: 116 LVETMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNE 175
           L   + + ++  K ++ITG +LGG+ A+L TLWLL      T   P LCITFGSPLIG+ 
Sbjct: 126 LKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIE-PTLKRP-LCITFGSPLIGDA 185

Query: 176 SLSRAIQRERWCGKFCHVVSNYDIMPRLLSTPLSSLSPKLHILFRYWHLSMASPHFGKLA 235
           SL + ++       F HVVS                                        
Sbjct: 186 SLQQILENSVRNSCFLHVVS---------------------------------------- 245

Query: 236 TQLTEREKEELFHVVLAHSNSISDLGEGSVRGQFWPFGNFFFCSEHGAICLDNAMSVLKM 295
                R K + F                       PFG F  C + G +C+++ ++V ++
Sbjct: 246 --AQTRIKMDFFK----------------------PFGTFLICFDSGCVCIEDHVAVTEL 305

Query: 296 LYLMLKTSAPNLSIEDHLNYGDHVRKVGVQYMERKNFNSLSLPNSSYEAGLALALQSAGI 355
           L  +  +   + S                Q + R + + LSL +S               
Sbjct: 306 LNGVHDSGLVDYS----------------QVLNRLDQSMLSLADSRL------------- 365

Query: 356 PFQDEVARMAEHSLRTASRIGQTPNLNAAKLAISLSKITPYRAEIEWYKASCEEADNQLG 415
                   + E  ++   +  +  NL    +   L+ +    A IEWYK  C+E   ++G
Sbjct: 366 --------IPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEV--KIG 425

Query: 416 YYDCFKKEDA--SVRHDRVNMNRHK--LATFWNRVIDMWENNELPPDFNTRAKWVNASQF 475
           YYD FK + A  S   D    N HK  L  FW  V++  E          + +++ +   
Sbjct: 426 YYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNN 485

Query: 476 YKLLVEPLDIAEYYRRDNHIVHGHYLNSGRERRYEIFDKWWRGREVTEEGNTQRMKYAS- 535
           Y+ ++EPLDIAEYY          Y  +GR   Y + +KW+    +  E    + +  S 
Sbjct: 486 YRRMIEPLDIAEYYLEGRK----EYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSD 514

Query: 536 -LTQDSCFWARLEEA----KDLLEIIKREGDVRKLAPIWKSLENFERYARGLIERKEVSK 595
            LT DSCFWA +E++      L   +    DVR++  + + L  FE Y   +I ++EVS 
Sbjct: 546 LLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREV--LTRKLVEFEGYVWEIITKREVSP 514

Query: 596 DVIAKNSSYTLWAQELRALK 606
           ++  + SS+  W +E + +K
Sbjct: 606 EIFLEESSFMKWWKEYKKIK 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7451.2e-10437.95Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1[more]
Q9SU726.8e-3626.48Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1[more]
Q9SU713.4e-3524.95Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1[more]
Q9XF231.7e-3425.69Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1[more]
Q4F8831.3e-3125.40Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SA... [more]
Match NameE-valueIdentityDescription
A0A0A0L3S40.0e+0094.58PAD4 OS=Cucumis sativus OX=3659 GN=Csa_4G496760 PE=4 SV=1[more]
A0A1S3B5S60.0e+0094.10lipase-like PAD4 OS=Cucumis melo OX=3656 GN=LOC103486086 PE=4 SV=1[more]
A0A6J1HT584.7e-29881.94lipase-like PAD4 OS=Cucurbita maxima OX=3661 GN=LOC111465963 PE=4 SV=1[more]
A0A6J1EHH05.7e-29681.77lipase-like PAD4 OS=Cucurbita moschata OX=3662 GN=LOC111432561 PE=4 SV=1[more]
A0A6J1D6Z01.2e-28279.21lipase-like PAD4 OS=Momordica charantia OX=3673 GN=LOC111017842 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_011653897.10.0e+0094.58lipase-like PAD4 [Cucumis sativus] >KGN54786.1 hypothetical protein Csa_012136 [... [more]
XP_008442139.10.0e+0094.10PREDICTED: lipase-like PAD4 [Cucumis melo][more]
XP_038881104.10.0e+0089.00lipase-like PAD4 [Benincasa hispida][more]
XP_022966244.19.6e-29881.94lipase-like PAD4 [Cucurbita maxima][more]
XP_022925275.11.2e-29581.77lipase-like PAD4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G52430.18.3e-10637.95alpha/beta-Hydrolases superfamily protein [more]
AT3G48090.14.8e-3726.48alpha/beta-Hydrolases superfamily protein [more]
AT3G48090.21.1e-3627.33alpha/beta-Hydrolases superfamily protein [more]
AT3G48080.12.4e-3624.95alpha/beta-Hydrolases superfamily protein [more]
AT5G14930.29.4e-3325.40senescence-associated gene 101 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041266EDS1, EP domainPFAMPF18117EDS1_EPcoord: 398..601
e-value: 1.6E-66
score: 223.9
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 100..204
e-value: 2.4E-20
score: 72.8
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 37..253
e-value: 5.5E-23
score: 83.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 102..207
NoneNo IPR availablePANTHERPTHR47413:SF2LIPASE-LIKE PAD4coord: 1..608
NoneNo IPR availablePANTHERPTHR47413LIPASE-LIKE PAD4coord: 1..608

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016141.1PI0016141.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010618 aerenchyma formation
biological_process GO:0071327 cellular response to trehalose stimulus
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0010225 response to UV-C
biological_process GO:0009625 response to insect
biological_process GO:0001666 response to hypoxia
biological_process GO:0080142 regulation of salicylic acid biosynthetic process
biological_process GO:2000022 regulation of jasmonic acid mediated signaling pathway
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0080151 positive regulation of salicylic acid mediated signaling pathway
biological_process GO:1900367 positive regulation of defense response to insect
biological_process GO:1900426 positive regulation of defense response to bacterium
biological_process GO:0010942 positive regulation of cell death
biological_process GO:1901183 positive regulation of camalexin biosynthetic process
biological_process GO:0009626 plant-type hypersensitive response
biological_process GO:0010105 negative regulation of ethylene-activated signaling pathway
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0006629 lipid metabolic process
biological_process GO:0010150 leaf senescence
biological_process GO:0060866 leaf abscission
biological_process GO:0050829 defense response to Gram-negative bacterium
cellular_component GO:0005829 cytosol
cellular_component GO:0106093 EDS1 disease-resistance complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus