PI0015539 (gene) Melon (PI 482460) v1

Overview
NamePI0015539
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr04: 5671953 .. 5673371 (+)
RNA-Seq ExpressionPI0015539
SyntenyPI0015539
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAGCAAGCAATCCAAAGCTCCATCAATGGATGAACAAATCGAAGTCCCCCACTATTTCCTCTGCCCCATCTCCCTCCAAATCATGAAAGACCCTGTAACTCTCCCCTCCGGCATCACCTACGATCGCCACTCCATTGAAACTTGGCTCTTCTCCGGCAAAAACTCCTCCTGCCCCGTCACTAAACTCCCCGTCTCCGACTCCGATTCCGATCTCCTCACCCCCAACCACACCCTCCGCCGTCTCATCCAAGCTTGGTGTACTTTGAACTCCTCCCATGGCGTCGAACGTTTCCCCACTCCTAAACCCCCCATTCATAAATCCCAAATCCTTCACCTTATTTCCACTTCCAACACTTCCCCTTCTTCCCTAATCTCCTCCATTCGTCGTCTCCGCTCCATTTCCGCCGAGTCTGAAACTAATCGCCGATGCGTTGAATTCGCCGGTGCGCCGGAGTTTTTAGCCTCTGTTATTGTGGATTCGGATTCCTCTGCTTCTCATGAAGCCCTCTCCACTCTACATAATCTCCGTCTCTCTGATTCAACGTTTAAATCATTGGCTACCCATCCTGAATTCCTCGAGTCCTTGACTGATTTCATGAAATTACAACAGGGTACTCATGAATCGTCCAGAACGTACGCGGTGTTGATTTTGAAATCGATTGTTGAAGTCGCCGACCCAATTCAACTAAGCTTCTTGAAATCCGAATTGTTTGTGCAAATCGTTGAGATTTTGAAAGATCGATCATCTTCTCAACAGATTTTCAAAGCGGCATTGGGTATTTTGATTGCGGTGAGTCCAATGGGGAGAAACAGACTGAAGGCAGTGGAAGCAGGTGGGGTTGGGGCTTTGGTTGAGATTTTACTGTCGTCGCCGGAAAAAAGAGTATGTGAAATGACATTGACGGCGATGGATTTACTGTGTGGGTGTGCAGATGGAAGAGCGGCGCTGTTGGCACACGGCGGAGGGATGGCGGTGGTTTCGAAGAAGATATTGAGAGTGTCGCAATTTGGGAGTGAAAGGGCGGTGAGGATATTGTATTGGGTGGCGAAATTCTCAGGAAGTCCGGCGGTGTTGATGGAAATGGCGCAATTGGGGATTGTGGCAAAGCTCTGTTTGGTTCTGCAAATTGAAAATGGAAGCAAGACGAAGGAGAAAGCTAAAGAGATTTTGAAAATGCATTCTCGTCTTTGGAAGAACTCACCTTGTATTCCTTCTAAATTGGCTTCTTCATATCCTACAAATTAAGGAATTTCTATCAACAAAAACAAGAAACCATTTTAAAACTATTTTTCCTTTTTCTTCTCATTTTTGTGTATATAATTCAATAGAATTGTAAACTATTAATTTTTAGTTAAATGAAAATCCAAAGGATCCACATTATTGTTTCTATGCAAAGCTTTGTCTTTATAATTTA

mRNA sequence

AAACAAGCAAGCAATCCAAAGCTCCATCAATGGATGAACAAATCGAAGTCCCCCACTATTTCCTCTGCCCCATCTCCCTCCAAATCATGAAAGACCCTGTAACTCTCCCCTCCGGCATCACCTACGATCGCCACTCCATTGAAACTTGGCTCTTCTCCGGCAAAAACTCCTCCTGCCCCGTCACTAAACTCCCCGTCTCCGACTCCGATTCCGATCTCCTCACCCCCAACCACACCCTCCGCCGTCTCATCCAAGCTTGGTGTACTTTGAACTCCTCCCATGGCGTCGAACGTTTCCCCACTCCTAAACCCCCCATTCATAAATCCCAAATCCTTCACCTTATTTCCACTTCCAACACTTCCCCTTCTTCCCTAATCTCCTCCATTCGTCGTCTCCGCTCCATTTCCGCCGAGTCTGAAACTAATCGCCGATGCGTTGAATTCGCCGGTGCGCCGGAGTTTTTAGCCTCTGTTATTGTGGATTCGGATTCCTCTGCTTCTCATGAAGCCCTCTCCACTCTACATAATCTCCGTCTCTCTGATTCAACGTTTAAATCATTGGCTACCCATCCTGAATTCCTCGAGTCCTTGACTGATTTCATGAAATTACAACAGGGTACTCATGAATCGTCCAGAACGTACGCGGTGTTGATTTTGAAATCGATTGTTGAAGTCGCCGACCCAATTCAACTAAGCTTCTTGAAATCCGAATTGTTTGTGCAAATCGTTGAGATTTTGAAAGATCGATCATCTTCTCAACAGATTTTCAAAGCGGCATTGGGTATTTTGATTGCGGTGAGTCCAATGGGGAGAAACAGACTGAAGGCAGTGGAAGCAGGTGGGGTTGGGGCTTTGGTTGAGATTTTACTGTCGTCGCCGGAAAAAAGAGTATGTGAAATGACATTGACGGCGATGGATTTACTGTGTGGGTGTGCAGATGGAAGAGCGGCGCTGTTGGCACACGGCGGAGGGATGGCGGTGGTTTCGAAGAAGATATTGAGAGTGTCGCAATTTGGGAGTGAAAGGGCGGTGAGGATATTGTATTGGGTGGCGAAATTCTCAGGAAGTCCGGCGGTGTTGATGGAAATGGCGCAATTGGGGATTGTGGCAAAGCTCTGTTTGGTTCTGCAAATTGAAAATGGAAGCAAGACGAAGGAGAAAGCTAAAGAGATTTTGAAAATGCATTCTCGTCTTTGGAAGAACTCACCTTGTATTCCTTCTAAATTGGCTTCTTCATATCCTACAAATTAAGGAATTTCTATCAACAAAAACAAGAAACCATTTTAAAACTATTTTTCCTTTTTCTTCTCATTTTTGTGTATATAATTCAATAGAATTGTAAACTATTAATTTTTAGTTAAATGAAAATCCAAAGGATCCACATTATTGTTTCTATGCAAAGCTTTGTCTTTATAATTTA

Coding sequence (CDS)

ATGGATGAACAAATCGAAGTCCCCCACTATTTCCTCTGCCCCATCTCCCTCCAAATCATGAAAGACCCTGTAACTCTCCCCTCCGGCATCACCTACGATCGCCACTCCATTGAAACTTGGCTCTTCTCCGGCAAAAACTCCTCCTGCCCCGTCACTAAACTCCCCGTCTCCGACTCCGATTCCGATCTCCTCACCCCCAACCACACCCTCCGCCGTCTCATCCAAGCTTGGTGTACTTTGAACTCCTCCCATGGCGTCGAACGTTTCCCCACTCCTAAACCCCCCATTCATAAATCCCAAATCCTTCACCTTATTTCCACTTCCAACACTTCCCCTTCTTCCCTAATCTCCTCCATTCGTCGTCTCCGCTCCATTTCCGCCGAGTCTGAAACTAATCGCCGATGCGTTGAATTCGCCGGTGCGCCGGAGTTTTTAGCCTCTGTTATTGTGGATTCGGATTCCTCTGCTTCTCATGAAGCCCTCTCCACTCTACATAATCTCCGTCTCTCTGATTCAACGTTTAAATCATTGGCTACCCATCCTGAATTCCTCGAGTCCTTGACTGATTTCATGAAATTACAACAGGGTACTCATGAATCGTCCAGAACGTACGCGGTGTTGATTTTGAAATCGATTGTTGAAGTCGCCGACCCAATTCAACTAAGCTTCTTGAAATCCGAATTGTTTGTGCAAATCGTTGAGATTTTGAAAGATCGATCATCTTCTCAACAGATTTTCAAAGCGGCATTGGGTATTTTGATTGCGGTGAGTCCAATGGGGAGAAACAGACTGAAGGCAGTGGAAGCAGGTGGGGTTGGGGCTTTGGTTGAGATTTTACTGTCGTCGCCGGAAAAAAGAGTATGTGAAATGACATTGACGGCGATGGATTTACTGTGTGGGTGTGCAGATGGAAGAGCGGCGCTGTTGGCACACGGCGGAGGGATGGCGGTGGTTTCGAAGAAGATATTGAGAGTGTCGCAATTTGGGAGTGAAAGGGCGGTGAGGATATTGTATTGGGTGGCGAAATTCTCAGGAAGTCCGGCGGTGTTGATGGAAATGGCGCAATTGGGGATTGTGGCAAAGCTCTGTTTGGTTCTGCAAATTGAAAATGGAAGCAAGACGAAGGAGAAAGCTAAAGAGATTTTGAAAATGCATTCTCGTCTTTGGAAGAACTCACCTTGTATTCCTTCTAAATTGGCTTCTTCATATCCTACAAATTAA

Protein sequence

MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRRLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN
Homology
BLAST of PI0015539 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 6.8e-117
Identity = 225/412 (54.61%), Postives = 306/412 (74.27%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDE+IE+P +FLCPISL+IMKDPV + +GITYDR SIE WLF+GK +SCPVTK  ++D+D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
              LTPNHTLRRLIQ+WCTLN+S+GVER PTP+PPI KS+I  LI  S +S  + +  ++
Sbjct: 66  ---LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLK 125

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVD--SDSSASHEALSTLHNLRLSDSTFKSLA 180
           RLR I +E+ TN+RC+E AG PEFLA+++ +   + S + EAL+ L++L  S++  K+L 
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLL 185

Query: 181 THPE---FLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEI 240
            + +    ++SLT  M  Q+G +E SR YA L+LK+I+EVADP+Q   LK E+F ++V+I
Sbjct: 186 NNKKDNNIVKSLTKIM--QRGMYE-SRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 245

Query: 241 LKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLS---SPEKRVCEMTL 300
           L DR  SQ+  KAA+ IL+ + P GRNR KAVEAG +  ++E+L+    + E+R  EM +
Sbjct: 246 LDDR-ISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAM 305

Query: 301 TAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLME 360
             +DLLC CA+GRA  L HG  +AVV KKILRVSQ  S+RAVR+L  V +F  +PA+L E
Sbjct: 306 VVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHE 365

Query: 361 MAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYP 405
           M QLG+VAKLCLVLQ+  G KTKEKAKE+LK+H+R+WK+SPC+P  +  +YP
Sbjct: 366 MLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of PI0015539 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.9e-112
Identity = 217/438 (49.54%), Postives = 295/438 (67.35%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MD++IE+P +FLCPISL IMKDPV + +GITYDR SIE WLFSGK +SCPVTK  ++++D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
              LTPNHTLRRLIQ+WCTLN+S+G+ER PTPKPPI KS+I  LI  S++S  + +  ++
Sbjct: 61  ---LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLK 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVI------------------------------- 180
           RLR I +E+ TN+RC+E A  PEFLA+++                               
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 181 VDSDSSASHEALSTLHNLRLSDSTFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLIL 240
            DS  S   EALS L++L  S++  KSL  + +    +    K+ Q     SR YA L+L
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 241 KSIVEVADPIQLSFLKSELFVQIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEA 300
           K ++EVADP+Q+  L+ ELF ++++IL D+  S +  ++A+ IL+   P GRNR KAVE 
Sbjct: 241 KKLLEVADPMQIILLERELFGEVIQILHDQ-ISHKATRSAMQILVITCPWGRNRHKAVEG 300

Query: 301 GGVGALVEILLS---SPEKRVCEMTLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVS 360
           G +  ++E+L+    S E+R  EM +  +D+LC CA+GRA  L HG  +AVVSKKILRVS
Sbjct: 301 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 360

Query: 361 QFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHS 405
           Q  SERAVR+L  V +F  +P++L EM QLG+VAKLCLVLQ+  G+KTKEKAKE+LK+H+
Sbjct: 361 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 420

BLAST of PI0015539 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.4e-60
Identity = 170/441 (38.55%), Postives = 231/441 (52.38%), Query Frame = 0

Query: 2   DEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDS 61
           +E+IE+P+YF+CPISL+IMKDPVT  SGITYDR +I  WL   K  SCPVTK P+   DS
Sbjct: 5   EEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPL-PLDS 64

Query: 62  DLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRR 121
           D LTPNH LRRLIQ WC  N + GV R  TP+ P  K  ++  I          +     
Sbjct: 65  D-LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 124

Query: 122 LRSIS--AESETNRRCVEFAGAPEFLASVIV-----DSDS----SASHEALSTLHNLRLS 181
           L+ +   A    NRR +   G  + L   +V     D D         E+L  LH + + 
Sbjct: 125 LQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 184

Query: 182 DSTFKS-LATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELF 241
            +  K+ L  +   +ESLT  +  +      S+ Y +++L+++ E      +  L  E+F
Sbjct: 185 SNDAKTILMENDRVMESLTWVLHQEDFL---SKAYTIVLLRNLTEYTSSHIVERLNPEIF 244

Query: 242 VQIVEILKD----------------RSSS------------------QQIFKAALGILIA 301
             I+  LKD                +SSS                  +Q   AAL IL+ 
Sbjct: 245 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 304

Query: 302 VSPMGRNRLKAVEAGGVGALVEILLS-SPEKRVCEMTLTAMDLLCGCADGRAALLAHGGG 361
            S   RNR   V+ G V  L+E+ +S + EKR+ E+ L  +  LC CA+GRA +LAH GG
Sbjct: 305 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 364

Query: 362 MAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGSKT 396
           +AVV+K++LRVS    +RA+ IL  V+KFS    V+ EM  +G V KLC VL ++ G   
Sbjct: 365 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 424

BLAST of PI0015539 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.3e-46
Identity = 132/399 (33.08%), Postives = 219/399 (54.89%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           +D  I++P++F CPISL++M+DPVT+ +G TYDR SIE+W+  G N++CPVT+ P+SD  
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF- 67

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLIST------SNTSPSS 120
              L PNHTLRRLIQ WC  N S+GVER PTPK P   + +  L+S       ++ S  S
Sbjct: 68  --TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 127

Query: 121 LISSIRRLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSAS--HEALSTLHNLRLSD- 180
             +++RRLR  + +S+ NR  +    A E L  ++    +S+    E+L+ L  L +++ 
Sbjct: 128 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEP 187

Query: 181 STFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSE-LFV 240
           + F S+++ P  +E LT  +             A++ + S    +  ++ S   SE +F 
Sbjct: 188 NQFVSISSDPGRVEFLTRLL-FDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 247

Query: 241 QIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEM 300
            ++++L++  SS++  K  +  L A+  +   R  A+ AG    L++ L +  ++   E 
Sbjct: 248 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 307

Query: 301 TLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVL 360
            L  ++LLC   +G AA   H   + ++ K ILRVS   +E A   L  +A  +      
Sbjct: 308 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEERWR 367

Query: 361 MEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLW 390
            E A  G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 368 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of PI0015539 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.1e-45
Identity = 132/412 (32.04%), Postives = 225/412 (54.61%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           +D  I++P++F CPISL +M DPVT+ +G TYDR SI++W+  G N++CPVT++ +SD  
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALSDF- 67

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLIST------SNTSPSS 120
              L PNHTLRRLIQ WC  N S+GVER PTPK P     +  L+S       ++ S  S
Sbjct: 68  --TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 127

Query: 121 LISSIRRLRSISAESETNRRCVEFAGAPEFLASVI---VDSDSSASH---EALSTLHNLR 180
             ++IRRLR ++ +SE NR  +    A E L  ++   +++ S +S    E+L+ L  L 
Sbjct: 128 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLH 187

Query: 181 LSDSTFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKS-- 240
           ++++  +++A+ P  +  +T   +L   +    R  A  +++ ++  A  + L  + S  
Sbjct: 188 MTETECEAVASDPSRVGFMT---RLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGS 247

Query: 241 -ELFVQIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEK 300
             +F  ++++LK+  SS++  K  +  + A+  + + R  A+ AG  G L++ L +  ++
Sbjct: 248 DSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDR 307

Query: 301 RVCEMTLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSG 360
              E  L  ++LLC   +G AA   H   + ++ K ILRVS   +E A   L  +A  + 
Sbjct: 308 CDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTA 367

Query: 361 SPAVLMEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPS 398
                 E A  G+V +L L++Q +   + K KA+ +LK+    W +   + S
Sbjct: 368 EERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of PI0015539 vs. ExPASy TrEMBL
Match: A0A5D3DNW0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004610 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.3e-214
Identity = 393/406 (96.80%), Postives = 399/406 (98.28%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCP+TKLPVS SD
Sbjct: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SDLLTPNHTLRRLIQAWCTLNSS+GVERFPTPKPPIHKSQILH+ISTSNTSPSSLISSIR
Sbjct: 61  SDLLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIR 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSISAESETNRRCVEFAGAPEFL SVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH
Sbjct: 121 RLRSISAESETNRRCVEFAGAPEFLVSVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
           PE LESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLK ELFVQIVEILKDRS
Sbjct: 181 PEVLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SS QIFKAALGILIAVSP+GRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG
Sbjct: 241 SSLQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CADGRAALLAHGGGMAVVSKKILRVSQ GSERAVRILY VAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLV+QIENGSKTKEKAKEILKMHSR+WKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of PI0015539 vs. ExPASy TrEMBL
Match: A0A1S3B6U7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.3e-214
Identity = 393/406 (96.80%), Postives = 399/406 (98.28%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCP+TKLPVS SD
Sbjct: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SDLLTPNHTLRRLIQAWCTLNSS+GVERFPTPKPPIHKSQILH+ISTSNTSPSSLISSIR
Sbjct: 61  SDLLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIR 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSISAESETNRRCVEFAGAPEFL SVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH
Sbjct: 121 RLRSISAESETNRRCVEFAGAPEFLVSVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
           PE LESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLK ELFVQIVEILKDRS
Sbjct: 181 PEVLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SS QIFKAALGILIAVSP+GRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG
Sbjct: 241 SSLQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CADGRAALLAHGGGMAVVSKKILRVSQ GSERAVRILY VAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLV+QIENGSKTKEKAKEILKMHSR+WKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of PI0015539 vs. ExPASy TrEMBL
Match: A0A0A0LB33 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE=4 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 3.7e-214
Identity = 392/406 (96.55%), Postives = 397/406 (97.78%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD
Sbjct: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILH+ISTSNTSPSS ISSIR
Sbjct: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIR 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSISAESETNRRCVEFAGAPEFL SVIV SDSSASHEALSTLHNLRLSDSTFKSLAT 
Sbjct: 121 RLRSISAESETNRRCVEFAGAPEFLVSVIVGSDSSASHEALSTLHNLRLSDSTFKSLATR 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
           PEFLESLTDFMKLQQGTHESSRTYAVLILKSI+EVA+PIQLSFLK ELFVQIVEILKDRS
Sbjct: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SSQQIFKAALGILIAVSP+GRNRLKAVEAGGV ALVEILLSSPEKRVCEMTLTAMD+LCG
Sbjct: 241 SSQQIFKAALGILIAVSPLGRNRLKAVEAGGVRALVEILLSSPEKRVCEMTLTAMDILCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CADGRAALLAHGGGMAVVSKKILRVSQ GSERAVRILY VAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLVLQIENG KTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVLQIENGGKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 406

BLAST of PI0015539 vs. ExPASy TrEMBL
Match: A0A6J1KXX6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.1e-162
Identity = 311/412 (75.49%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCP+TKLP+  +DSD
Sbjct: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPI-PADSD 61

Query: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRRL 122
            LTPNHTLRRLIQAWCTL+S HG+ERFPTPKPPI+K QI  LIS+S+++PSSL+S IRRL
Sbjct: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121

Query: 123 RSISAESETNRRCVEFAGAPEFLASVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182
           RS++AESE+NRRC+E AGAPEFLASVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 181

Query: 183 KSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVE 242
           KSL  + E  +SLT  M  +QGT+E SR YA L+LKS++EVADP++L+FLKSELFV+IV 
Sbjct: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241

Query: 243 ILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302
           ILKD+ +SQQ  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 242 ILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301

Query: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMA 362
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ GSERAVRIL+ VAK+SGSPAVL EMA
Sbjct: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361

Query: 363 QLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           QLGIVAKLCLVLQ+E+GSKTKEKAKEILKMH+R+W+NS CIPSKLASSYP N
Sbjct: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of PI0015539 vs. ExPASy TrEMBL
Match: A0A6J1GKE4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.9e-162
Identity = 310/412 (75.24%), Postives = 356/412 (86.41%), Query Frame = 0

Query: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCP+TKLP+  +DSD
Sbjct: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSNKNASCPITKLPI-PADSD 61

Query: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRRL 122
            LTPNHTLRRLIQAWCTL+S HG+ERFPTPKPPI+K QI  LIS+S+++PSSL+S IRRL
Sbjct: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121

Query: 123 RSISAESETNRRCVEFAGAPEFLASVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182
           RS++AESE+NRRC+E AGAPEFLASVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 181

Query: 183 KSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVE 242
           KSL  + E  +SLT  M  +QGT+E SR YA L+ KS++EVADP++L+FLKSELFV+IV 
Sbjct: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLFKSLLEVADPMELTFLKSELFVEIVG 241

Query: 243 ILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302
           ILKD+ +SQQ  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 242 ILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301

Query: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMA 362
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ GSERAVRIL+ VAK+SGSPAVL EMA
Sbjct: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361

Query: 363 QLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           QLGIVAKLCLVLQ+E+GSKTKEKAKEILKMH+R+W+NS CIPSKLASSYP N
Sbjct: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of PI0015539 vs. NCBI nr
Match: XP_008442921.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo] >KAA0043809.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK25323.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa])

HSP 1 Score: 755.4 bits (1949), Expect = 2.6e-214
Identity = 393/406 (96.80%), Postives = 399/406 (98.28%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCP+TKLPVS SD
Sbjct: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SDLLTPNHTLRRLIQAWCTLNSS+GVERFPTPKPPIHKSQILH+ISTSNTSPSSLISSIR
Sbjct: 61  SDLLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIR 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSISAESETNRRCVEFAGAPEFL SVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH
Sbjct: 121 RLRSISAESETNRRCVEFAGAPEFLVSVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
           PE LESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLK ELFVQIVEILKDRS
Sbjct: 181 PEVLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SS QIFKAALGILIAVSP+GRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG
Sbjct: 241 SSLQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CADGRAALLAHGGGMAVVSKKILRVSQ GSERAVRILY VAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLV+QIENGSKTKEKAKEILKMHSR+WKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of PI0015539 vs. NCBI nr
Match: XP_004136517.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN59215.1 hypothetical protein Csa_001842 [Cucumis sativus])

HSP 1 Score: 753.8 bits (1945), Expect = 7.5e-214
Identity = 392/406 (96.55%), Postives = 397/406 (97.78%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD
Sbjct: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILH+ISTSNTSPSS ISSIR
Sbjct: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIR 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSISAESETNRRCVEFAGAPEFL SVIV SDSSASHEALSTLHNLRLSDSTFKSLAT 
Sbjct: 121 RLRSISAESETNRRCVEFAGAPEFLVSVIVGSDSSASHEALSTLHNLRLSDSTFKSLATR 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
           PEFLESLTDFMKLQQGTHESSRTYAVLILKSI+EVA+PIQLSFLK ELFVQIVEILKDRS
Sbjct: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SSQQIFKAALGILIAVSP+GRNRLKAVEAGGV ALVEILLSSPEKRVCEMTLTAMD+LCG
Sbjct: 241 SSQQIFKAALGILIAVSPLGRNRLKAVEAGGVRALVEILLSSPEKRVCEMTLTAMDILCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CADGRAALLAHGGGMAVVSKKILRVSQ GSERAVRILY VAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLVLQIENG KTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVLQIENGGKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 406

BLAST of PI0015539 vs. NCBI nr
Match: XP_038903290.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 713.0 bits (1839), Expect = 1.5e-201
Identity = 369/406 (90.89%), Postives = 388/406 (95.57%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           M+++IEVPHYFLCPISLQIMKDPV LPSGITYDRHSIETWLFS KNS CPVTKLP+SDSD
Sbjct: 1   MEDEIEVPHYFLCPISLQIMKDPVILPSGITYDRHSIETWLFSNKNSFCPVTKLPISDSD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
           SD LTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLIS++NTSPSSLISSI 
Sbjct: 61  SDFLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISSANTSPSSLISSIH 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSASHEALSTLHNLRLSDSTFKSLATH 180
           RLRSIS ESETNRRCVE AGAPEFLASVIVD+DSSAS+EALSTLHNLRLSDSTFK LATH
Sbjct: 121 RLRSISDESETNRRCVESAGAPEFLASVIVDTDSSASYEALSTLHNLRLSDSTFKRLATH 180

Query: 181 PEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEILKDRS 240
            EFL+SLT+FMKL +GT ESSR YAVLILKSIVEVADPIQLSFLKS+LFVQIVEILKDRS
Sbjct: 181 SEFLDSLTNFMKL-RGTQESSRAYAVLILKSIVEVADPIQLSFLKSDLFVQIVEILKDRS 240

Query: 241 SSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTAMDLLCG 300
           SSQQIFKAALGILIAV P GRNR+KAVEAGGVG LVEILL++PE+RVCEMTL AMDLLCG
Sbjct: 241 SSQQIFKAALGILIAVCPWGRNRVKAVEAGGVGVLVEILLATPERRVCEMTLMAMDLLCG 300

Query: 301 CADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360
           CA+GRAALL HGGGMAVVSKKILRVSQ GSERAVRILYWVAKFSGSPAVLMEMAQLGIVA
Sbjct: 301 CAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILYWVAKFSGSPAVLMEMAQLGIVA 360

Query: 361 KLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           KLCLVLQIENGSKTKEKAKEILKMH+R+WKNSPCIPSKLASSYPTN
Sbjct: 361 KLCLVLQIENGSKTKEKAKEILKMHARVWKNSPCIPSKLASSYPTN 405

BLAST of PI0015539 vs. NCBI nr
Match: XP_023540239.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.6 bits (1516), Expect = 4.2e-164
Identity = 312/412 (75.73%), Postives = 358/412 (86.89%), Query Frame = 0

Query: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCP+TKLP+  +DSD
Sbjct: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPI-PADSD 61

Query: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRRL 122
            LTPNHTLRRLIQAWCTL+S HG+ERFPTPKPPI+K QI  LIS+S+++PSSL+S IRRL
Sbjct: 62  SLTPNHTLRRLIQAWCTLHSDHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121

Query: 123 RSISAESETNRRCVEFAGAPEFLASVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182
           RS++AESE+NRRC+E AGAPEFLASVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 181

Query: 183 KSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVE 242
           KSL  + E  +SLT  M  +QGT+E SR YA L+LKS++EVADP++L+FLKSELFV+IV 
Sbjct: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241

Query: 243 ILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302
           ILKD+ +SQQ  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 242 ILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMVLTA 301

Query: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMA 362
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ GSERAVRIL+WVAK+SGSPAVL EMA
Sbjct: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFWVAKYSGSPAVLTEMA 361

Query: 363 QLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           QLGIVAKLCLVLQ+E+GSKTKEKAKEILKMH+R+W+NS CIPSKLASSYP N
Sbjct: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of PI0015539 vs. NCBI nr
Match: XP_023005635.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 582.8 bits (1501), Expect = 2.3e-162
Identity = 311/412 (75.49%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCP+TKLP+  +DSD
Sbjct: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPI-PADSD 61

Query: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRRL 122
            LTPNHTLRRLIQAWCTL+S HG+ERFPTPKPPI+K QI  LIS+S+++PSSL+S IRRL
Sbjct: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121

Query: 123 RSISAESETNRRCVEFAGAPEFLASVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182
           RS++AESE+NRRC+E AGAPEFLASVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 181

Query: 183 KSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVE 242
           KSL  + E  +SLT  M  +QGT+E SR YA L+LKS++EVADP++L+FLKSELFV+IV 
Sbjct: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241

Query: 243 ILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302
           ILKD+ +SQQ  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 242 ILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301

Query: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMA 362
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ GSERAVRIL+ VAK+SGSPAVL EMA
Sbjct: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361

Query: 363 QLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 407
           QLGIVAKLCLVLQ+E+GSKTKEKAKEILKMH+R+W+NS CIPSKLASSYP N
Sbjct: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of PI0015539 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 422.2 bits (1084), Expect = 4.8e-118
Identity = 225/412 (54.61%), Postives = 306/412 (74.27%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MDE+IE+P +FLCPISL+IMKDPV + +GITYDR SIE WLF+GK +SCPVTK  ++D+D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
              LTPNHTLRRLIQ+WCTLN+S+GVER PTP+PPI KS+I  LI  S +S  + +  ++
Sbjct: 66  ---LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLK 125

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVIVD--SDSSASHEALSTLHNLRLSDSTFKSLA 180
           RLR I +E+ TN+RC+E AG PEFLA+++ +   + S + EAL+ L++L  S++  K+L 
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLL 185

Query: 181 THPE---FLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELFVQIVEI 240
            + +    ++SLT  M  Q+G +E SR YA L+LK+I+EVADP+Q   LK E+F ++V+I
Sbjct: 186 NNKKDNNIVKSLTKIM--QRGMYE-SRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 245

Query: 241 LKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLS---SPEKRVCEMTL 300
           L DR  SQ+  KAA+ IL+ + P GRNR KAVEAG +  ++E+L+    + E+R  EM +
Sbjct: 246 LDDR-ISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAM 305

Query: 301 TAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLME 360
             +DLLC CA+GRA  L HG  +AVV KKILRVSQ  S+RAVR+L  V +F  +PA+L E
Sbjct: 306 VVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHE 365

Query: 361 MAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPSKLASSYP 405
           M QLG+VAKLCLVLQ+  G KTKEKAKE+LK+H+R+WK+SPC+P  +  +YP
Sbjct: 366 MLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of PI0015539 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 406.4 bits (1043), Expect = 2.8e-113
Identity = 217/438 (49.54%), Postives = 295/438 (67.35%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           MD++IE+P +FLCPISL IMKDPV + +GITYDR SIE WLFSGK +SCPVTK  ++++D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIR 120
              LTPNHTLRRLIQ+WCTLN+S+G+ER PTPKPPI KS+I  LI  S++S  + +  ++
Sbjct: 61  ---LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLK 120

Query: 121 RLRSISAESETNRRCVEFAGAPEFLASVI------------------------------- 180
           RLR I +E+ TN+RC+E A  PEFLA+++                               
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 181 VDSDSSASHEALSTLHNLRLSDSTFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLIL 240
            DS  S   EALS L++L  S++  KSL  + +    +    K+ Q     SR YA L+L
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 241 KSIVEVADPIQLSFLKSELFVQIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEA 300
           K ++EVADP+Q+  L+ ELF ++++IL D+  S +  ++A+ IL+   P GRNR KAVE 
Sbjct: 241 KKLLEVADPMQIILLERELFGEVIQILHDQ-ISHKATRSAMQILVITCPWGRNRHKAVEG 300

Query: 301 GGVGALVEILLS---SPEKRVCEMTLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVS 360
           G +  ++E+L+    S E+R  EM +  +D+LC CA+GRA  L HG  +AVVSKKILRVS
Sbjct: 301 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 360

Query: 361 QFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHS 405
           Q  SERAVR+L  V +F  +P++L EM QLG+VAKLCLVLQ+  G+KTKEKAKE+LK+H+
Sbjct: 361 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 420

BLAST of PI0015539 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 233.8 bits (595), Expect = 2.4e-61
Identity = 170/441 (38.55%), Postives = 231/441 (52.38%), Query Frame = 0

Query: 2   DEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDS 61
           +E+IE+P+YF+CPISL+IMKDPVT  SGITYDR +I  WL   K  SCPVTK P+   DS
Sbjct: 19  EEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPL-PLDS 78

Query: 62  DLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLISTSNTSPSSLISSIRR 121
           D LTPNH LRRLIQ WC  N + GV R  TP+ P  K  ++  I          +     
Sbjct: 79  D-LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 138

Query: 122 LRSIS--AESETNRRCVEFAGAPEFLASVIV-----DSDS----SASHEALSTLHNLRLS 181
           L+ +   A    NRR +   G  + L   +V     D D         E+L  LH + + 
Sbjct: 139 LQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 198

Query: 182 DSTFKS-LATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSELF 241
            +  K+ L  +   +ESLT  +  +      S+ Y +++L+++ E      +  L  E+F
Sbjct: 199 SNDAKTILMENDRVMESLTWVLHQEDFL---SKAYTIVLLRNLTEYTSSHIVERLNPEIF 258

Query: 242 VQIVEILKD----------------RSSS------------------QQIFKAALGILIA 301
             I+  LKD                +SSS                  +Q   AAL IL+ 
Sbjct: 259 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 318

Query: 302 VSPMGRNRLKAVEAGGVGALVEILLS-SPEKRVCEMTLTAMDLLCGCADGRAALLAHGGG 361
            S   RNR   V+ G V  L+E+ +S + EKR+ E+ L  +  LC CA+GRA +LAH GG
Sbjct: 319 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 378

Query: 362 MAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGSKT 396
           +AVV+K++LRVS    +RA+ IL  V+KFS    V+ EM  +G V KLC VL ++ G   
Sbjct: 379 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 438

BLAST of PI0015539 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 188.7 bits (478), Expect = 9.0e-48
Identity = 132/399 (33.08%), Postives = 219/399 (54.89%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           +D  I++P++F CPISL++M+DPVT+ +G TYDR SIE+W+  G N++CPVT+ P+SD  
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF- 67

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLIST------SNTSPSS 120
              L PNHTLRRLIQ WC  N S+GVER PTPK P   + +  L+S       ++ S  S
Sbjct: 68  --TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 127

Query: 121 LISSIRRLRSISAESETNRRCVEFAGAPEFLASVIVDSDSSAS--HEALSTLHNLRLSD- 180
             +++RRLR  + +S+ NR  +    A E L  ++    +S+    E+L+ L  L +++ 
Sbjct: 128 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEP 187

Query: 181 STFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKSE-LFV 240
           + F S+++ P  +E LT  +             A++ + S    +  ++ S   SE +F 
Sbjct: 188 NQFVSISSDPGRVEFLTRLL-FDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 247

Query: 241 QIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEKRVCEM 300
            ++++L++  SS++  K  +  L A+  +   R  A+ AG    L++ L +  ++   E 
Sbjct: 248 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 307

Query: 301 TLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSGSPAVL 360
            L  ++LLC   +G AA   H   + ++ K ILRVS   +E A   L  +A  +      
Sbjct: 308 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEERWR 367

Query: 361 MEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLW 390
            E A  G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 368 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of PI0015539 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 185.7 bits (470), Expect = 7.6e-47
Identity = 132/412 (32.04%), Postives = 225/412 (54.61%), Query Frame = 0

Query: 1   MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSD 60
           +D  I++P++F CPISL +M DPVT+ +G TYDR SI++W+  G N++CPVT++ +SD  
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALSDF- 67

Query: 61  SDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHLIST------SNTSPSS 120
              L PNHTLRRLIQ WC  N S+GVER PTPK P     +  L+S       ++ S  S
Sbjct: 68  --TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 127

Query: 121 LISSIRRLRSISAESETNRRCVEFAGAPEFLASVI---VDSDSSASH---EALSTLHNLR 180
             ++IRRLR ++ +SE NR  +    A E L  ++   +++ S +S    E+L+ L  L 
Sbjct: 128 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLH 187

Query: 181 LSDSTFKSLATHPEFLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKS-- 240
           ++++  +++A+ P  +  +T   +L   +    R  A  +++ ++  A  + L  + S  
Sbjct: 188 MTETECEAVASDPSRVGFMT---RLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGS 247

Query: 241 -ELFVQIVEILKDRSSSQQIFKAALGILIAVSPMGRNRLKAVEAGGVGALVEILLSSPEK 300
             +F  ++++LK+  SS++  K  +  + A+  + + R  A+ AG  G L++ L +  ++
Sbjct: 248 DSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDR 307

Query: 301 RVCEMTLTAMDLLCGCADGRAALLAHGGGMAVVSKKILRVSQFGSERAVRILYWVAKFSG 360
              E  L  ++LLC   +G AA   H   + ++ K ILRVS   +E A   L  +A  + 
Sbjct: 308 CDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTA 367

Query: 361 SPAVLMEMAQLGIVAKLCLVLQIENGSKTKEKAKEILKMHSRLWKNSPCIPS 398
                 E A  G+V +L L++Q +   + K KA+ +LK+    W +   + S
Sbjct: 368 EERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TG36.8e-11754.61E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC63.9e-11249.54E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF153.4e-6038.55E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT791.3e-4633.08U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA41.1e-4532.04U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5D3DNW01.3e-21496.80RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B6U71.3e-21496.80RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 ... [more]
A0A0A0LB333.7e-21496.55RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE... [more]
A0A6J1KXX61.1e-16275.49RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 P... [more]
A0A6J1GKE41.9e-16275.24RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050... [more]
Match NameE-valueIdentityDescription
XP_008442921.12.6e-21496.80PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo] >KAA0043809.1 E... [more]
XP_004136517.17.5e-21496.55E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN59215.1 hypothetical pro... [more]
XP_038903290.11.5e-20190.89E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_023540239.14.2e-16475.73E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_023005635.12.3e-16275.49E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G35930.14.8e-11854.61plant U-box 23 [more]
AT3G52450.12.8e-11349.54plant U-box 22 [more]
AT3G11840.12.4e-6138.55plant U-box 24 [more]
AT3G19380.19.0e-4833.08plant U-box 25 [more]
AT1G49780.17.6e-4732.04plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 10..77
e-value: 4.8E-30
score: 115.8
IPR003613U box domainPFAMPF04564U-boxcoord: 7..81
e-value: 7.4E-20
score: 70.9
IPR003613U box domainPROSITEPS51698U_BOXcoord: 6..84
score: 35.932999
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..88
e-value: 1.8E-27
score: 97.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 95..392
e-value: 1.7E-20
score: 74.8
NoneNo IPR availablePANTHERPTHR22849:SF132E3 UBIQUITIN-PROTEIN LIGASE PUB23coord: 3..404
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 3..404
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 11..53
e-value: 2.03246E-20
score: 81.8963
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..85
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 101..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015539.1PI0015539.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity