PI0015173 (gene) Melon (PI 482460) v1

Overview
NamePI0015173
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEF hand calcium-binding protein family
Locationchr06: 14058754 .. 14059723 (+)
RNA-Seq ExpressionPI0015173
SyntenyPI0015173
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGAAAAACAAACTCTCTCTCTCTCTCTCTCTCTATTCAAATTTCAAATCACCTTCACACAATCTACCCCATGGCCAAGAACAACCACTCCCTTTCAATTTCAAATCCAGGATCTTCTTCCTTAGGCTCCATGGACGAAATCATCAAAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACCGAACTCGGCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGAAGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGACGAGTTCACCGACTTCACCTCCTCTTCCACTGGTGGTAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAGTGTTCATCGCCAGTAGAGAGGTAGTTTCACAAATTTAATCAATTTTAGGCTTAGAATTTCGAAACCAAAATTGAGTATTATCAGTTGATTCTTAGTGTGTACAGTCTATGAATCTATCCTTTCTTTCAGGGTTTGTAACCGATTTAGGGCACTAAAACGTCTCTACAATGAATTTCTCCTCTGGTTTTAGATCTTAATCGGAGATCCGAAAAGAAAAAAAAAACAAACAGATTATAAGTTCATATCATCTCTCTCTAATTGATTCCGATTTTCTGTTTCTGAAAGGGATGGATGAATCCGAAGTTGAGGGAGTAAATTTTGTGTGCTGAATTTGTGGAATATTTGATTGATTGATATAGTTACTGAAGGAATTATTTGCATCGGTTAGATTATTTATAAACATAATTTGGTAATTTCTTCAAA

mRNA sequence

AAAAAGAAAAACAAACTCTCTCTCTCTCTCTCTCTCTATTCAAATTTCAAATCACCTTCACACAATCTACCCCATGGCCAAGAACAACCACTCCCTTTCAATTTCAAATCCAGGATCTTCTTCCTTAGGCTCCATGGACGAAATCATCAAAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACCGAACTCGGCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGAAGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGACGAGTTCACCGACTTCACCTCCTCTTCCACTGGTGGTAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAGTGTTCATCGCCAGTAGAGAGGTAGTTTCACAAATTTAATCAATTTTAGGCTTAGAATTTCGAAACCAAAATTGAGTATTATCAGTTGATTCTTAGTGTGTACAGTCTATGAATCTATCCTTTCTTTCAGGGTTTGTAACCGATTTAGGGCACTAAAACGTCTCTACAATGAATTTCTCCTCTGGTTTTAGATCTTAATCGGAGATCCGAAAAGAAAAAAAAAACAAACAGATTATAAGTTCATATCATCTCTCTCTAATTGATTCCGATTTTCTGTTTCTGAAAGGGATGGATGAATCCGAAGTTGAGGGAGTAAATTTTGTGTGCTGAATTTGTGGAATATTTGATTGATTGATATAGTTACTGAAGGAATTATTTGCATCGGTTAGATTATTTATAAACATAATTTGGTAATTTCTTCAAA

Coding sequence (CDS)

ATGGCCAAGAACAACCACTCCCTTTCAATTTCAAATCCAGGATCTTCTTCCTTAGGCTCCATGGACGAAATCATCAAAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACCGAACTCGGCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGAAGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGACGAGTTCACCGACTTCACCTCCTCTTCCACTGGTGGTAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Protein sequence

MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Homology
BLAST of PI0015173 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.0e-41
Identity = 88/148 (59.46%), Postives = 107/148 (72.30%), Query Frame = 0

Query: 12  NPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDG 71
           N   + LGSM++I KVF +FDKN DGKIS+ EL   +G LS N S EE   +M E D DG
Sbjct: 4   NVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDG 63

Query: 72  DGFIDLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCS 131
           +GFIDLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS
Sbjct: 64  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 123

Query: 132 LKDCCRMISSVDVDGDGHVNFEEFKKMM 155
           ++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 124 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of PI0015173 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-40
Identity = 88/147 (59.86%), Postives = 105/147 (71.43%), Query Frame = 0

Query: 16  SSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDGDGFI 75
           S LGSMD+I KVF +FDKNGDGKISV EL   +  LS   S EE   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 76  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 135
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 136 DCCRMISSVDVDGDGHVNFEEFKKMMT 156
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

BLAST of PI0015173 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 3.8e-39
Identity = 82/157 (52.23%), Postives = 109/157 (69.43%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA  N   +   P +  + + +E+ KVF++FD NGDGKISV ELG     +  + +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           +R++ E+D D DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL R
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

BLAST of PI0015173 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 6.6e-36
Identity = 80/140 (57.14%), Postives = 97/140 (69.29%), Query Frame = 0

Query: 22  DEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDGDGFIDLDEFT 81
           +E+  VFN+FD NGDGKIS  EL   L  L  N S EEI RIM EID D DGFI++ EF 
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 82  DFTSS------STGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 141
            F  +      S+GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 142 SVDVDGDGHVNFEEFKKMMT 156
           SVD DGDG+V+FEEFKKMMT
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

BLAST of PI0015173 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 4.3e-35
Identity = 78/151 (51.66%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 5   NHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIM 64
           N +L  +N  ++    M E+ KVF+KFD NGDGKISV+ELG     +  + + EE++R++
Sbjct: 3   NTNLESTNKSTTPSTDM-ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVL 62

Query: 65  SEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEK 124
            EID D DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  
Sbjct: 63  DEIDIDCDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMT 122

Query: 125 CSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 156
           CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 123 CSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

BLAST of PI0015173 vs. ExPASy TrEMBL
Match: A0A5A7UHX3 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001160 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.6e-77
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA NN SLSIS PGSSSLGSMDE+I+VFNKFDKNGDGKISV+ELGAALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

BLAST of PI0015173 vs. ExPASy TrEMBL
Match: A0A1S3C5W9 (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.6e-77
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA NN SLSIS PGSSSLGSMDE+I+VFNKFDKNGDGKISV+ELGAALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

BLAST of PI0015173 vs. ExPASy TrEMBL
Match: A0A0A0LCM1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 151/157 (96.18%), Postives = 154/157 (98.09%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MAKNN SLSIS PGSS+LGSMDEII+VFNKFDKNGDGKISVTEL AALGELSGNIST+EI
Sbjct: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

BLAST of PI0015173 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 126/156 (80.77%), Postives = 139/156 (89.10%), Query Frame = 0

Query: 9   SISNPGSS----SLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIM 68
           S+S+P S+    +LGSMDE+ KVFNKFDKNGDGKISV ELG ALGEL G +S++E+ RIM
Sbjct: 7   SVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIM 66

Query: 69  SEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 128
           +EIDKDGDGFIDLDEF +F    S++ GGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 67  AEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 126

Query: 129 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 127 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of PI0015173 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.2e-61
Identity = 126/156 (80.77%), Postives = 137/156 (87.82%), Query Frame = 0

Query: 9   SISNPGS----SSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIM 68
           S+SNP S     +LGSMDE+I VF KFDKNGDGKIS+ EL AALGEL G IS +EI RIM
Sbjct: 7   SVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEIRRIM 66

Query: 69  SEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 128
           SEIDKDG+GFIDLDEF +F   +SS+ GG+KDLQDAFDLYD+DKNGLISAKELHSVLKRL
Sbjct: 67  SEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSVLKRL 126

Query: 129 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 127 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of PI0015173 vs. NCBI nr
Match: XP_008457051.1 (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK01546.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 297.4 bits (760), Expect = 7.5e-77
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA NN SLSIS PGSSSLGSMDE+I+VFNKFDKNGDGKISV+ELGAALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

BLAST of PI0015173 vs. NCBI nr
Match: XP_004145834.1 (probable calcium-binding protein CML23 [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-76
Identity = 151/157 (96.18%), Postives = 154/157 (98.09%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MAKNN SLSIS PGSS+LGSMDEII+VFNKFDKNGDGKISVTEL AALGELSGNIST+EI
Sbjct: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

BLAST of PI0015173 vs. NCBI nr
Match: KAE8650604.1 (hypothetical protein Csa_010664 [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 7.2e-72
Identity = 140/144 (97.22%), Postives = 143/144 (99.31%), Query Frame = 0

Query: 14  GSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDGDG 73
           GSS+LGSMDEII+VFNKFDKNGDGKISVTEL AALGELSGNIST+EIHRIMSEIDKDGDG
Sbjct: 6   GSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDG 65

Query: 74  FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 133
           FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM
Sbjct: 66  FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 125

Query: 134 ISSVDVDGDGHVNFEEFKKMMTRS 158
           ISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 126 ISSVDVDGDGHVNFEEFKKMMTRS 149

BLAST of PI0015173 vs. NCBI nr
Match: XP_038879983.1 (probable calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 270.4 bits (690), Expect = 9.8e-69
Identity = 141/160 (88.12%), Postives = 147/160 (91.88%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MAK N+SLSISNPG SSLGSMD++IKVFNKFDKNGDGKISV ELGAAL EL G IST+EI
Sbjct: 1   MAKKNNSLSISNPG-SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 159

BLAST of PI0015173 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 249.2 bits (635), Expect = 2.3e-62
Identity = 126/156 (80.77%), Postives = 139/156 (89.10%), Query Frame = 0

Query: 9   SISNPGSS----SLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIM 68
           S+S+P S+    +LGSMDE+ KVFNKFDKNGDGKISV ELG ALGEL G +S++E+ RIM
Sbjct: 7   SVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIM 66

Query: 69  SEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 128
           +EIDKDGDGFIDLDEF +F    S++ GGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 67  AEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 126

Query: 129 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 127 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of PI0015173 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 169.1 bits (427), Expect = 2.9e-42
Identity = 88/148 (59.46%), Postives = 107/148 (72.30%), Query Frame = 0

Query: 12  NPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDG 71
           N   + LGSM++I KVF +FDKN DGKIS+ EL   +G LS N S EE   +M E D DG
Sbjct: 4   NVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDG 63

Query: 72  DGFIDLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCS 131
           +GFIDLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS
Sbjct: 64  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 123

Query: 132 LKDCCRMISSVDVDGDGHVNFEEFKKMM 155
           ++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 124 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of PI0015173 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 167.5 bits (423), Expect = 8.3e-42
Identity = 88/147 (59.86%), Postives = 105/147 (71.43%), Query Frame = 0

Query: 16  SSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIMSEIDKDGDGFI 75
           S LGSMD+I KVF +FDKNGDGKISV EL   +  LS   S EE   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 76  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 135
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 136 DCCRMISSVDVDGDGHVNFEEFKKMMT 156
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

BLAST of PI0015173 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-40
Identity = 82/157 (52.23%), Postives = 109/157 (69.43%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA  N   +   P +  + + +E+ KVF++FD NGDGKISV ELG     +  + +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           +R++ E+D D DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL R
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

BLAST of PI0015173 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-40
Identity = 82/157 (52.23%), Postives = 109/157 (69.43%), Query Frame = 0

Query: 1   MAKNNHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEI 60
           MA  N   +   P +  + + +E+ KVF++FD NGDGKISV ELG     +  + +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           +R++ E+D D DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL R
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 158
           LG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

BLAST of PI0015173 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 149.1 bits (375), Expect = 3.1e-36
Identity = 78/151 (51.66%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 5   NHSLSISNPGSSSLGSMDEIIKVFNKFDKNGDGKISVTELGAALGELSGNISTEEIHRIM 64
           N +L  +N  ++    M E+ KVF+KFD NGDGKISV+ELG     +  + + EE++R++
Sbjct: 3   NTNLESTNKSTTPSTDM-ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVL 62

Query: 65  SEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEK 124
            EID D DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  
Sbjct: 63  DEIDIDCDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMT 122

Query: 125 CSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 156
           CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 123 CSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8Y14.0e-4159.46Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
P250701.2e-4059.86Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2[more]
Q9LE223.8e-3952.23Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
Q9M7R06.6e-3657.14Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Q9C9U84.3e-3551.66Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UHX33.6e-7795.54Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3C5W93.6e-7795.54probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 P... [more]
A0A0A0LCM11.4e-7696.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1[more]
A0A6J1CNU41.1e-6280.77probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1G9H51.2e-6180.77probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
XP_008457051.17.5e-7795.54PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 p... [more]
XP_004145834.12.8e-7696.18probable calcium-binding protein CML23 [Cucumis sativus][more]
KAE8650604.17.2e-7297.22hypothetical protein Csa_010664 [Cucumis sativus][more]
XP_038879983.19.8e-6988.13probable calcium-binding protein CML23 [Benincasa hispida][more]
XP_022143269.12.3e-6280.77probable calcium-binding protein CML23 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G66400.12.9e-4259.46calmodulin like 23 [more]
AT5G37770.18.3e-4259.86EF hand calcium-binding protein family [more]
AT1G18210.12.7e-4052.23Calcium-binding EF-hand family protein [more]
AT1G18210.22.7e-4052.23Calcium-binding EF-hand family protein [more]
AT1G73630.13.1e-3651.66EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 59..87
e-value: 2.1E-5
score: 34.0
coord: 93..121
e-value: 3.2E-5
score: 33.4
coord: 23..51
e-value: 1.4E-5
score: 34.5
coord: 129..157
e-value: 2.1E-4
score: 30.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 23..84
e-value: 2.3E-13
score: 50.4
coord: 92..154
e-value: 1.0E-11
score: 45.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 89..124
score: 14.318138
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 55..83
score: 10.105932
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 19..54
score: 14.820255
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 125..157
score: 13.174425
IPR002048EF-hand domainCDDcd00051EFhcoord: 93..155
e-value: 5.67235E-19
score: 73.7361
IPR002048EF-hand domainCDDcd00051EFhcoord: 23..83
e-value: 3.59907E-18
score: 71.8101
NoneNo IPR availableGENE3D1.10.238.10coord: 88..157
e-value: 8.5E-23
score: 82.4
NoneNo IPR availableGENE3D1.10.238.10coord: 14..87
e-value: 4.3E-21
score: 77.4
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 16..156
NoneNo IPR availablePANTHERPTHR10891:SF794CALCIUM-BINDING PROTEIN CML23-RELATEDcoord: 16..156
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 102..114
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 138..150
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 32..44
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 21..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015173.1PI0015173.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding