Homology
BLAST of PI0012249 vs. ExPASy Swiss-Prot
Match:
Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)
HSP 1 Score: 467.2 bits (1201), Expect = 2.4e-130
Identity = 230/511 (45.01%), Postives = 335/511 (65.56%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C H NN +N L++T Y ILN +I+NLRF TPKPL I+TP +
Sbjct: 32 ENFLKCFSKHIP-NNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S +QA++ C K LQIRTRSGGHD EG SY+S VPFV++DL ++SI IDV ++AWV+
Sbjct: 92 SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
+GAT+GE+YY I++K++ L FP G+ PT+G+GG SGGG+G ++R YGL DN++DAHLV
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWD-QKAA 241
+ +G+V+DR SMGEDLFWAIRGGGG +FGI+ AWK+KLV +PS T F++ K +
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGR---IMEASFFSLFLGKANKLLSLMQK 301
L ++WQ +A + D+DL + T N ++ G+ + F S+F G + L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331
Query: 302 TFPELGLKKEDCLEMSWVESMAFMGSGFVSTNNV----KLLLNRTPLSNDRYKTKSDYAT 361
+FPELG+KK DC E SW+++ F SG V+ N ++LL+R+ + K DY
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIFY-SGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVK 391
Query: 362 EPISKTALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTW 421
+PI +TA+ + ++ +E++ +L P+G A ++ Y +W
Sbjct: 392 KPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW 451
Query: 422 QRRNDDLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGH 481
+++ D+ KH+ W R +Y++ TP+VS++PR AY+NY+DLD+G N P +Y +A +WG
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWGE 511
Query: 482 RYFGNNFERLMEVKTKVDPCNFFRHEQSIPP 489
+YFG NF RL++VKTKVDP NFFR+EQSIPP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPP 539
BLAST of PI0012249 vs. ExPASy Swiss-Prot
Match:
Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)
HSP 1 Score: 466.5 bits (1199), Expect = 4.0e-130
Identity = 237/511 (46.38%), Postives = 332/511 (64.97%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C+ S N+ +S +IHT SY ILN SI+N RF +TPKP++IITP A
Sbjct: 35 ENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 94
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVS-NVPFVLIDLIYLNSIAIDVEDESAWV 121
+ VQ+++ C + H + IRTRSGGHD+EG SY++ + PFV+IDL L SI +DV++ + WV
Sbjct: 95 TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 154
Query: 122 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 181
QSGAT+GELYY I K S++L FPAG PT+GIGG GGG+G ++RKYGL DNV+DAH+
Sbjct: 155 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 214
Query: 182 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 241
VD NG +DR MGED FWAIRGGGG SF +VL+WK++L+ +PS+VT F + KT +++A
Sbjct: 215 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 274
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 301
+++++WQY+A +V DLFI + + ASF L+LG + LL+LM+ FP
Sbjct: 275 SIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKFP 334
Query: 302 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTA 361
ELGL+ +C EMSW+ES+ + F+ ++++L R S +K K D+ EPI KTA
Sbjct: 335 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 394
Query: 362 LEGMWKRFNDEELETVQLMLIPFGA----------AYP------IQIGYYVTWQRRND-- 421
++ +W+RF E +++L PFG +P +I Y W D
Sbjct: 395 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 454
Query: 422 ---DLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRY 481
K+L W +Y++MTP+VSKSPR AY+N++D+D+G + T YEEA VWG +Y
Sbjct: 455 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 514
Query: 482 FGNNFERLMEVKTKVDPCNFFRHEQSIPPSK 491
F NNF+RL+ VKT VDP +FF EQSIP K
Sbjct: 515 FKNNFDRLVRVKTNVDPMDFFCDEQSIPIMK 530
BLAST of PI0012249 vs. ExPASy Swiss-Prot
Match:
Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)
HSP 1 Score: 462.6 bits (1189), Expect = 5.8e-129
Identity = 223/511 (43.64%), Postives = 334/511 (65.36%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C + NN +N I+T Y +LN +I+NLRF+ TPKPL I+TP +
Sbjct: 32 ENFLKCFSEYIP-NNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNV 91
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S +QAS+ C K LQIRTRSGGHD EG SY+S VPF ++DL ++++ +D+ ++AWV+
Sbjct: 92 SHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVE 151
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
+GAT+GE+YY I++ ++ FP G+ PT+G+GG SGGG+G ++R YGL DN++DAHLV
Sbjct: 152 AGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWD-QKAA 241
+ +G+V+DR SMGEDLFWAIRGGGG +FGI+ AWK+KLV +PS T F++ K +
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLV 271
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGR---IMEASFFSLFLGKANKLLSLMQK 301
L ++WQ +A + D+DL ++T N ++ G+ + F S+FLG + L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNK 331
Query: 302 TFPELGLKKEDCLEMSWVESMAFMGSGFVSTNNV----KLLLNRTPLSNDRYKTKSDYAT 361
+FPELG+KK DC E+SW+++ F SG V+ N ++LL+R+ + K DY
Sbjct: 332 SFPELGIKKTDCKELSWIDTTIFY-SGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVK 391
Query: 362 EPISKTALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTW 421
+ I +TA+ + ++ +EE+ +L P+G A ++ Y TW
Sbjct: 392 KLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATW 451
Query: 422 QRRNDDLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGH 481
+++ D+ KH+ W R +Y++ TP+VS++PR AY+NY+DLD+G N + P +Y +A +WG
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPES-PNNYTQARIWGE 511
Query: 482 RYFGNNFERLMEVKTKVDPCNFFRHEQSIPP 489
+YFG NF RL++VKTK DP NFFR+EQSIPP
Sbjct: 512 KYFGKNFNRLVKVKTKADPNNFFRNEQSIPP 539
BLAST of PI0012249 vs. ExPASy Swiss-Prot
Match:
A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)
HSP 1 Score: 461.1 bits (1185), Expect = 1.7e-128
Identity = 224/511 (43.84%), Postives = 339/511 (66.34%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C + N +N+ L++T Y ILN +I+NLRF+ TPKPL IITP +
Sbjct: 32 ENFLKCFSQYIP-TNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNV 91
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S +Q ++ C K LQIRTRSGGHD EG SY+S VPFV++DL ++S+ IDV ++AWV+
Sbjct: 92 SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
+GAT+GE+YY I++ ++ L FPAG+ PT+G GG SGGG+G ++R YGL DN++DAHLV
Sbjct: 152 AGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLV 211
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWD-QKAA 241
+ +G+V+DR SMGEDLFWAIRGGGG +FGI+ AWK++LV +PS+ T F++ K + +
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGR---IMEASFFSLFLGKANKLLSLMQK 301
LV++WQ +A +++L + T N ++ G+ + + F S+F G + L+ LM K
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331
Query: 302 TFPELGLKKEDCLEMSWVESMAFMGSGFVSTNNV----KLLLNRTPLSNDRYKTKSDYAT 361
+FPELG+KK DC ++SW++++ F SG V+ N ++LL+R+ + K DY
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIFY-SGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVK 391
Query: 362 EPISKTALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTW 421
+PI +TA+ + ++ +E++ + P+G A +I Y +W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASW 451
Query: 422 QRRNDDLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGH 481
+++ D+ KH+ W R +Y++ TP+VS++PR AY+NY+DLD+G N + P +Y +A +WG
Sbjct: 452 EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGE 511
Query: 482 RYFGNNFERLMEVKTKVDPCNFFRHEQSIPP 489
+YFG NF RL++VKTKVDP NFFR+EQSIPP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539
BLAST of PI0012249 vs. ExPASy Swiss-Prot
Match:
A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)
HSP 1 Score: 461.1 bits (1185), Expect = 1.7e-128
Identity = 223/511 (43.64%), Postives = 339/511 (66.34%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C+ + N +N+ L++T Y ILN +++NLRF+ TPKPL I TP +
Sbjct: 32 ENFLKCLSQYIP-TNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S +Q ++ C K LQIRTRSGGHD EG SY+S VPFV++DL ++S+ IDV ++AWV+
Sbjct: 92 SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
SGAT+GE+YY I++ ++ L FPAG+ PT+G GG SGGG+G ++R YGL DN++DAHLV
Sbjct: 152 SGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLV 211
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWD-QKAA 241
+ +G+V+DR SMGEDLFWAIRGGGG +FGI+ AWK++LV +PS+ T F++ K + +
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGR---IMEASFFSLFLGKANKLLSLMQK 301
LV++WQ +A +++L + T N ++ G+ + + F S+F G + L+ LM K
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331
Query: 302 TFPELGLKKEDCLEMSWVESMAFMGSGFVSTNNV----KLLLNRTPLSNDRYKTKSDYAT 361
+FPELG+KK DC ++SW++++ F SG V+ N ++LL+R+ + K DY
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIFY-SGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVK 391
Query: 362 EPISKTALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTW 421
+PI +TA+ + ++ +E++ + P+G A +I Y +W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASW 451
Query: 422 QRRNDDLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGH 481
+++ D+ KH+ W R +Y++ TP+VS++PR AY+NY+DLD+G N + P +Y +A +WG
Sbjct: 452 EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGE 511
Query: 482 RYFGNNFERLMEVKTKVDPCNFFRHEQSIPP 489
+YFG NF RL++VKTKVDP NFFR+EQSIPP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539
BLAST of PI0012249 vs. ExPASy TrEMBL
Match:
A0A5D3D0K7 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00130 PE=3 SV=1)
HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 406/506 (80.24%), Postives = 452/506 (89.33%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
MEDFV+CI+VHSSNNNDSNSTLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP H
Sbjct: 1 MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI IDVEDE+AWV
Sbjct: 61 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TLGFPAGF TIG+GGFLSGGG GMMVRKYGLG DNVVDA++
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NGRVV+R SMGEDLFWAIRGGGGGSFGIVLAWKL+LVQ+PSIVTSFALHKTWDQ AA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS-EGGGRIMEASFFSLFLGKANKLLSLMQKTF 300
NL++RWQY+AP VD+DLFIS WVTASNSS + GRIMEASFFSLFLG A +LLSLM+KTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300
Query: 301 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 360
PELGLKK+DCLEMSWVESMAF SGFV +++LLL+RTP+ N RYKTKSDYATEPISKT
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
AL+GMW+RF DEELETVQL+LIPFG A YPI IGYY+TWQ+ N D
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
++LEW REL+DYMTPFVSKSPRAAY+NY+DLD+GTNN+DG+PT Y+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPPS 490
FERLMEVK KVDP NFFRHEQSIPP+
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPA 506
BLAST of PI0012249 vs. ExPASy TrEMBL
Match:
A0A1S3CIZ3 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103501494 PE=3 SV=1)
HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 406/506 (80.24%), Postives = 452/506 (89.33%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
MEDFV+CI+VHSSNNNDSNSTLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP H
Sbjct: 1 MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI IDVEDE+AWV
Sbjct: 61 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TLGFPAGF TIG+GGFLSGGG GMMVRKYGLG DNVVDA++
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NGRVV+R SMGEDLFWAIRGGGGGSFGIVLAWKL+LVQ+PSIVTSFALHKTWDQ AA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS-EGGGRIMEASFFSLFLGKANKLLSLMQKTF 300
NL++RWQY+AP VD+DLFIS WVTASNSS + GRIMEASFFSLFLG A +LLSLM+KTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300
Query: 301 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 360
PELGLKK+DCLEMSWVESMAF SGFV +++LLL+RTP+ N RYKTKSDYATEPISKT
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
AL+GMW+RF DEELETVQL+LIPFG A YPI IGYY+TWQ+ N D
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
++LEW REL+DYMTPFVSKSPRAAY+NY+DLD+GTNN+DG+PT Y+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPPS 490
FERLMEVK KVDP NFFRHEQSIPP+
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPA 506
BLAST of PI0012249 vs. ExPASy TrEMBL
Match:
A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)
HSP 1 Score: 771.2 bits (1990), Expect = 2.8e-219
Identity = 374/507 (73.77%), Postives = 438/507 (86.39%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
++DF++CI +HSSN++ SNSTLIHTPSSSSY ++LNFSIRNLRF+E TPKP+AI+TP+
Sbjct: 49 LQDFLHCISLHSSNHH-SNSTLIHTPSSSSYPHVLNFSIRNLRFAESHTPKPVAIVTPWQ 108
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
S+VQA+V CCK+H LQIRTRSGGHDFEG SYVSNVPFVLIDLI LNSI IDV++E+AW
Sbjct: 109 PSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLIDLINLNSITIDVDNETAWA 168
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TL FPAGF +IG+GGF SGGG GM+VRKYGL VDNVVDA+L
Sbjct: 169 QSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 228
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NG+++DRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV++P VTSFA+HK+WD AA
Sbjct: 229 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 288
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS--EGGGRIMEASFFSLFLGKANKLLSLMQKT 300
L+HRWQYVAP+VD DLFI+ WVTASNSS EGGGR+M+ASFFSLFLGKA +L+SLM+K
Sbjct: 289 KLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKASFFSLFLGKAAELVSLMKKA 348
Query: 301 FPELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISK 360
FPEL LKKEDCLE+SWVE+MAF S +VS N++LLL+RTPL RYK KSDYAT+PIS+
Sbjct: 349 FPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTPLITGRYKMKSDYATKPISE 408
Query: 361 TALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRND 420
TAL G+W+RF EELETVQL+LIPFG A++PI IGYYVTW R +
Sbjct: 409 TALNGIWERFKIEELETVQLILIPFGGKMNEISETATPNPHRASHPIHIGYYVTWHRPDA 468
Query: 421 DLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGN 480
D +HLEWTREL+DYMTPFVS+SPRAAYMNYKDLDIG NNEDGIPTSY+EASVWG+RYFG
Sbjct: 469 DSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRNNEDGIPTSYDEASVWGYRYFGI 528
Query: 481 NFERLMEVKTKVDPCNFFRHEQSIPPS 490
NFERL+EVKTKVDP NFF+HE+SIPP+
Sbjct: 529 NFERLVEVKTKVDPFNFFQHEESIPPA 554
BLAST of PI0012249 vs. ExPASy TrEMBL
Match:
A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)
HSP 1 Score: 766.1 bits (1977), Expect = 9.1e-218
Identity = 374/505 (74.06%), Postives = 428/505 (84.75%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
++DF++CI +HSSN+N SNSTLIHTPSSSSY Y+LNFSIRNLRF+E TPKP+AI+TP+
Sbjct: 35 LQDFLHCISLHSSNHN-SNSTLIHTPSSSSYLYVLNFSIRNLRFAESDTPKPVAIVTPWQ 94
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCK+H LQIRTRSGG DFEG SYVSNVPFVLIDLI LNSI ID EDE+AW
Sbjct: 95 ASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLIDLINLNSITIDTEDETAWA 154
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TL FPAG +G+GGF SGGGFGM++RKYGL VDNVVDA L
Sbjct: 155 QSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGFGMLLRKYGLAVDNVVDAQL 214
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NG+++DRDSMGEDLFWAIRGGGGGSFGIVL WKLKLV++P VTSFA+HK+WD AA
Sbjct: 215 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVRVPPTVTSFAVHKSWDLNAA 274
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 300
L+H+WQYVAPEVD DLFI+ WVTASNSS+GGGRIM+ASFF+LFLG+A +L+SLM K FP
Sbjct: 275 KLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFFALFLGEAAELVSLMAKAFP 334
Query: 301 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNR-TPLSNDRYKTKSDYATEPISKT 360
EL L +EDCLEMSWVESMAF S FVS N++LLL+R T LSN RYK KSDYATEPIS+T
Sbjct: 335 ELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRTTQLSNGRYKIKSDYATEPISET 394
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
AL G+W+RF EELET+QL+LIPFG A +PI IGYY TW R + D
Sbjct: 395 ALNGIWERFKIEELETLQLILIPFGGKMNEISETDTPNPHRARHPIHIGYYATWHRPDAD 454
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
+HLEWTREL+DYMTPFVSKSPRAAY+N KDLDIG NNEDGIPTSYEEAS+WGH+YFG N
Sbjct: 455 SRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNEDGIPTSYEEASIWGHQYFGIN 514
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPP 489
FERL+EVKTK DP NFFRHEQSIPP
Sbjct: 515 FERLVEVKTKADPFNFFRHEQSIPP 538
BLAST of PI0012249 vs. ExPASy TrEMBL
Match:
A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)
HSP 1 Score: 765.4 bits (1975), Expect = 1.5e-217
Identity = 375/505 (74.26%), Postives = 432/505 (85.54%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
+EDF++CI +HSSN + SNSTLIHTPSSSSY Y+LNFSIRNLRF+E TPKP+AI+TP+
Sbjct: 44 LEDFLHCISLHSSNRH-SNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQ 103
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCK+H LQIRTRSGGHDFEG SYVSN+PFVLIDLI LNSI IDV+DE+AW
Sbjct: 104 ASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWA 163
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TL FPAG +IG+GGF SGGG GM+VRKYGL VDNVVDA+L
Sbjct: 164 QSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 223
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NG+++DRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV++P VTSFA+HK+WD AA
Sbjct: 224 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 283
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 300
L+H WQYVA EVD DLFI+ VTASNSS+ GGRIM+ASFFSLFLGKA +L+SLM+K FP
Sbjct: 284 KLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLMEKAFP 343
Query: 301 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTA 360
EL LKKEDCLE+SWVE+MAF S +VS N++LLL+RT L RYK KSDYAT+PIS+TA
Sbjct: 344 ELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKPISETA 403
Query: 361 LEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDDL 420
L G+W+RF EELETVQL+LIPFG A++PI IGYYVTW R + D
Sbjct: 404 LNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPDADS 463
Query: 421 KHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNNF 480
+HLEWTREL+DYMTPFVS+SPRAAYMNYKDLDIG NNEDGIPTSY+EASVWG+RYFG NF
Sbjct: 464 RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRYFGINF 523
Query: 481 ERLMEVKTKVDPCNFFRHEQSIPPS 490
ERL+EVKTKVDP NFFRHEQSIPP+
Sbjct: 524 ERLVEVKTKVDPFNFFRHEQSIPPA 547
BLAST of PI0012249 vs. NCBI nr
Match:
XP_038894011.1 (berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida])
HSP 1 Score: 867.1 bits (2239), Expect = 7.8e-248
Identity = 421/506 (83.20%), Postives = 457/506 (90.32%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
EDFV+CI +HSSNNNDSN+TLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP HA
Sbjct: 31 EDFVHCISLHSSNNNDSNTTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSHA 90
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S VQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI ID EDESAWVQ
Sbjct: 91 SHVQAAVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDTEDESAWVQ 150
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
SGAT+G+LYYRI++KSQTLGFPAGFAPTIG+GG+LSGGGFGMMVRKYGL VDNVVDA++V
Sbjct: 151 SGATIGKLYYRIAEKSQTLGFPAGFAPTIGVGGYLSGGGFGMMVRKYGLAVDNVVDAYVV 210
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAAN 241
D NGR+ DR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV +P IVTSFALHKTWDQ AA+
Sbjct: 211 DANGRIFDRHSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHKTWDQNAAD 270
Query: 242 LVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFPE 301
L+HRWQYVAPEVD DLFI+ WVTASNSS+ GGRIMEASFFSLFLGKA+KLLSLMQKTFPE
Sbjct: 271 LIHRWQYVAPEVDEDLFITAWVTASNSSKEGGRIMEASFFSLFLGKADKLLSLMQKTFPE 330
Query: 302 LGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTAL 361
LGLKKEDCLEMSW+ESMAFM SGFVS NNVKLLL+RTPLSNDRYKTKSDYAT+PISKTAL
Sbjct: 331 LGLKKEDCLEMSWIESMAFMASGFVSANNVKLLLDRTPLSNDRYKTKSDYATKPISKTAL 390
Query: 362 EGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDDLK 421
EGMW+RF EELETVQLMLIPFG A YPIQIGYYVTWQ + DL+
Sbjct: 391 EGMWERFKIEELETVQLMLIPFGGKMNEISETETPFAYRAGYPIQIGYYVTWQDPDADLR 450
Query: 422 HLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNNFE 481
HL+WT+EL+DYMTPFVSKSPR AY+NYKDLDIGTN EDGIPTSYEEA VWGH YFGNNFE
Sbjct: 451 HLKWTQELHDYMTPFVSKSPRGAYINYKDLDIGTNKEDGIPTSYEEAGVWGHPYFGNNFE 510
Query: 482 RLMEVKTKVDPCNFFRHEQSIPPSKI 492
RL+EVK KVDP NFFRHEQSIPP+K+
Sbjct: 511 RLVEVKMKVDPFNFFRHEQSIPPAKM 536
BLAST of PI0012249 vs. NCBI nr
Match:
XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])
HSP 1 Score: 862.4 bits (2227), Expect = 1.9e-246
Identity = 421/507 (83.04%), Postives = 457/507 (90.14%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
EDFV+CI +HSSNNNDSN+TLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP HA
Sbjct: 31 EDFVHCISLHSSNNNDSNTTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSHA 90
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 121
S VQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI ID EDESAWVQ
Sbjct: 91 SHVQAAVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDTEDESAWVQ 150
Query: 122 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 181
SGAT+G+LYYRI++KSQTLGFPAGFAPTIG+GG+LSGGGFGMMVRKYGL VDNVVDA++V
Sbjct: 151 SGATIGKLYYRIAEKSQTLGFPAGFAPTIGVGGYLSGGGFGMMVRKYGLAVDNVVDAYVV 210
Query: 182 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAAN 241
D NGR+ DR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV +P IVTSFALHKTWDQ AA+
Sbjct: 211 DANGRIFDRHSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHKTWDQNAAD 270
Query: 242 LVHRWQYVAPEVDRDLFISTWVT-ASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 301
L+HRWQYVAPEVD DLFI+ WVT ASNSS+ GGRIMEASFFSLFLGKA+KLLSLMQKTFP
Sbjct: 271 LIHRWQYVAPEVDEDLFITAWVTAASNSSKEGGRIMEASFFSLFLGKADKLLSLMQKTFP 330
Query: 302 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTA 361
ELGLKKEDCLEMSW+ESMAFM SGFVS NNVKLLL+RTPLSNDRYKTKSDYAT+PISKTA
Sbjct: 331 ELGLKKEDCLEMSWIESMAFMASGFVSANNVKLLLDRTPLSNDRYKTKSDYATKPISKTA 390
Query: 362 LEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDDL 421
LEGMW+RF EELETVQLMLIPFG A YPIQIGYYVTWQ + DL
Sbjct: 391 LEGMWERFKIEELETVQLMLIPFGGKMNEISETETPFAYRAGYPIQIGYYVTWQDPDADL 450
Query: 422 KHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNNF 481
+HL+WT+EL+DYMTPFVSKSPR AY+NYKDLDIGTN EDGIPTSYEEA VWGH YFGNNF
Sbjct: 451 RHLKWTQELHDYMTPFVSKSPRGAYINYKDLDIGTNKEDGIPTSYEEAGVWGHPYFGNNF 510
Query: 482 ERLMEVKTKVDPCNFFRHEQSIPPSKI 492
ERL+EVK KVDP NFFRHEQSIPP+K+
Sbjct: 511 ERLVEVKMKVDPFNFFRHEQSIPPAKM 537
BLAST of PI0012249 vs. NCBI nr
Match:
XP_004147307.2 (berberine bridge enzyme-like 27 [Cucumis sativus])
HSP 1 Score: 843.6 bits (2178), Expect = 9.2e-241
Identity = 411/506 (81.23%), Postives = 453/506 (89.53%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
M+DFVNCI+VHSSNNNDSNSTLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP H
Sbjct: 30 MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 89
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI IDVEDESAWV
Sbjct: 90 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 149
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELY+RI +KS+TLGFPAGFA TIG+GGFLSGGGFGMMVRKYGLG DNVVDA++
Sbjct: 150 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 209
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VDGNGRVV+R SMGEDLFWAIRGGGGGSFGIVLAWKL+LVQ+PSIVTSFALHK WDQ AA
Sbjct: 210 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 269
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS-EGGGRIMEASFFSLFLGKANKLLSLMQKTF 300
NL++RWQY+AP VD+DLFIS WVTASNSS +G GRIMEASFFSLFLG A +LLSLM+KTF
Sbjct: 270 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 329
Query: 301 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 360
PELGLKKEDCLE SWVESMAF SGFVS +++LLL+RTPL N RYKTKSDYATEPIS+T
Sbjct: 330 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 389
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
LEGMW+RF DEELETVQL+LIPFG A YPI IGYY+TWQR + D
Sbjct: 390 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 449
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
KHL+W REL++YMTPFVSKSPRAAY+NY+DLD+GTNN+DG+PT EEAS+WGHRYFGNN
Sbjct: 450 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 509
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPPS 490
FERLMEVK KVDP NFFRHEQSIPP+
Sbjct: 510 FERLMEVKRKVDPFNFFRHEQSIPPA 535
BLAST of PI0012249 vs. NCBI nr
Match:
KAE8652747.1 (hypothetical protein Csa_013703 [Cucumis sativus])
HSP 1 Score: 843.6 bits (2178), Expect = 9.2e-241
Identity = 411/506 (81.23%), Postives = 453/506 (89.53%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
M+DFVNCI+VHSSNNNDSNSTLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP H
Sbjct: 289 MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 348
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI IDVEDESAWV
Sbjct: 349 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 408
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELY+RI +KS+TLGFPAGFA TIG+GGFLSGGGFGMMVRKYGLG DNVVDA++
Sbjct: 409 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 468
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VDGNGRVV+R SMGEDLFWAIRGGGGGSFGIVLAWKL+LVQ+PSIVTSFALHK WDQ AA
Sbjct: 469 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 528
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS-EGGGRIMEASFFSLFLGKANKLLSLMQKTF 300
NL++RWQY+AP VD+DLFIS WVTASNSS +G GRIMEASFFSLFLG A +LLSLM+KTF
Sbjct: 529 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 588
Query: 301 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 360
PELGLKKEDCLE SWVESMAF SGFVS +++LLL+RTPL N RYKTKSDYATEPIS+T
Sbjct: 589 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 648
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
LEGMW+RF DEELETVQL+LIPFG A YPI IGYY+TWQR + D
Sbjct: 649 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 708
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
KHL+W REL++YMTPFVSKSPRAAY+NY+DLD+GTNN+DG+PT EEAS+WGHRYFGNN
Sbjct: 709 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 768
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPPS 490
FERLMEVK KVDP NFFRHEQSIPP+
Sbjct: 769 FERLMEVKRKVDPFNFFRHEQSIPPA 794
BLAST of PI0012249 vs. NCBI nr
Match:
XP_008463307.2 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK17150.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])
HSP 1 Score: 834.7 bits (2155), Expect = 4.3e-238
Identity = 406/506 (80.24%), Postives = 452/506 (89.33%), Query Frame = 0
Query: 1 MEDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 60
MEDFV+CI+VHSSNNNDSNSTLIHTPSSSSYSY+LNFSIRNLRFSEP+TPKP+AIITP H
Sbjct: 1 MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60
Query: 61 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWV 120
ASQVQA+V CCKSH LQIRTRSGGHDFEG SYV+NVPFVLIDLI LNSI IDVEDE+AWV
Sbjct: 61 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120
Query: 121 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 180
QSGATVGELYY+I +KS+TLGFPAGF TIG+GGFLSGGG GMMVRKYGLG DNVVDA++
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
Query: 181 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 240
VD NGRVV+R SMGEDLFWAIRGGGGGSFGIVLAWKL+LVQ+PSIVTSFALHKTWDQ AA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
Query: 241 NLVHRWQYVAPEVDRDLFISTWVTASNSS-EGGGRIMEASFFSLFLGKANKLLSLMQKTF 300
NL++RWQY+AP VD+DLFIS WVTASNSS + GRIMEASFFSLFLG A +LLSLM+KTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300
Query: 301 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 360
PELGLKK+DCLEMSWVESMAF SGFV +++LLL+RTP+ N RYKTKSDYATEPISKT
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360
Query: 361 ALEGMWKRFNDEELETVQLMLIPFG----------------AAYPIQIGYYVTWQRRNDD 420
AL+GMW+RF DEELETVQL+LIPFG A YPI IGYY+TWQ+ N D
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420
Query: 421 LKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNN 480
++LEW REL+DYMTPFVSKSPRAAY+NY+DLD+GTNN+DG+PT Y+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480
Query: 481 FERLMEVKTKVDPCNFFRHEQSIPPS 490
FERLMEVK KVDP NFFRHEQSIPP+
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPA 506
BLAST of PI0012249 vs. TAIR 10
Match:
AT5G44410.1 (FAD-binding Berberine family protein )
HSP 1 Score: 466.5 bits (1199), Expect = 2.9e-131
Identity = 237/511 (46.38%), Postives = 332/511 (64.97%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E+F+ C+ S N+ +S +IHT SY ILN SI+N RF +TPKP++IITP A
Sbjct: 35 ENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 94
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVS-NVPFVLIDLIYLNSIAIDVEDESAWV 121
+ VQ+++ C + H + IRTRSGGHD+EG SY++ + PFV+IDL L SI +DV++ + WV
Sbjct: 95 TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 154
Query: 122 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 181
QSGAT+GELYY I K S++L FPAG PT+GIGG GGG+G ++RKYGL DNV+DAH+
Sbjct: 155 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 214
Query: 182 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 241
VD NG +DR MGED FWAIRGGGG SF +VL+WK++L+ +PS+VT F + KT +++A
Sbjct: 215 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 274
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 301
+++++WQY+A +V DLFI + + ASF L+LG + LL+LM+ FP
Sbjct: 275 SIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKFP 334
Query: 302 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTA 361
ELGL+ +C EMSW+ES+ + F+ ++++L R S +K K D+ EPI KTA
Sbjct: 335 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 394
Query: 362 LEGMWKRFNDEELETVQLMLIPFGA----------AYP------IQIGYYVTWQRRND-- 421
++ +W+RF E +++L PFG +P +I Y W D
Sbjct: 395 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 454
Query: 422 ---DLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRY 481
K+L W +Y++MTP+VSKSPR AY+N++D+D+G + T YEEA VWG +Y
Sbjct: 455 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 514
Query: 482 FGNNFERLMEVKTKVDPCNFFRHEQSIPPSK 491
F NNF+RL+ VKT VDP +FF EQSIP K
Sbjct: 515 FKNNFDRLVRVKTNVDPMDFFCDEQSIPIMK 530
BLAST of PI0012249 vs. TAIR 10
Match:
AT4G20820.1 (FAD-binding Berberine family protein )
HSP 1 Score: 459.9 bits (1182), Expect = 2.7e-129
Identity = 236/503 (46.92%), Postives = 326/503 (64.81%), Query Frame = 0
Query: 4 FVNCIHVHSSNNNDSN--STLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 63
F+ C+ S NDSN S +IHTP+ +S+S +L SI+N RFS P PKP+ I+TP
Sbjct: 37 FLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQP 96
Query: 64 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNVPFVLIDLIYLNSIAIDVEDESAWVQ 123
S VQ++V C + + IRTRSGGHD+EG SYV++ PFV++DL L SI +DV++ S WVQ
Sbjct: 97 SDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQ 156
Query: 124 SGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHLV 183
+GAT+GELYY I KK++TL FPAG PT+G+GG SGGG+G ++RK+GL D+V+DA +V
Sbjct: 157 TGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVV 216
Query: 184 DGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAAN 243
D GR+++R MGED FWAIRGGGG SF +VL+WK+ L+ +PS VT F + K +Q A
Sbjct: 217 DARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALK 276
Query: 244 LVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFPE 303
++HRWQ+VA +V DLFI + + ++ ASF L+LG LL ++ K FPE
Sbjct: 277 IIHRWQFVADKVSDDLFIRVMLQRYKN------MVRASFPGLYLGSVKNLLKMVNKEFPE 336
Query: 304 LGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTAL 363
LGL+++DC EMSW+ES+ + + + +L RT S +K KSD+ EP+ KTA+
Sbjct: 337 LGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASL-AFKAKSDFVQEPMPKTAI 396
Query: 364 EGMWKRFNDEELETVQLMLIPFG----------AAYPIQIG-----YYVTWQRRNDDLKH 423
+W+R + E E QL+ PFG +P + G Y+ + R + K+
Sbjct: 397 SKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKEKY 456
Query: 424 LEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTS-YEEASVWGHRYFGNNFE 483
+ W +YD M+ FV+KSPR AY+N +DLD+G G+ S YEE WG +YF NNFE
Sbjct: 457 MRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFE 516
Query: 484 RLMEVKTKVDPCNFFRHEQSIPP 489
RL+ VKT VDP +FF EQSIPP
Sbjct: 517 RLVRVKTSVDPSDFFCDEQSIPP 525
BLAST of PI0012249 vs. TAIR 10
Match:
AT1G30700.1 (FAD-binding Berberine family protein )
HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 226/502 (45.02%), Postives = 328/502 (65.34%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
E F C+ +S+ S I + SYS +L +IRNLRF+ TPKP II H
Sbjct: 26 ETFTQCL-TSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATHE 85
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYV--SNVPFVLIDLIYLNSIAIDVEDESAW 121
S VQA++ C K HNLQ++ RSGGHD++G SYV S PF ++D+ L S+ +DV ++AW
Sbjct: 86 SHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTAW 145
Query: 122 VQSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAH 181
VQ+GA +GE+YY I +KS+TL +PAG PT+G+GG +SGGG+G M+RKYGL VDN +DA
Sbjct: 146 VQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDAR 205
Query: 182 LVDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKA 241
+VD NG+++DR MGEDL+WAI GGGGGS+G+VLA+K+ LV++P VT F + +T +Q A
Sbjct: 206 MVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQNA 265
Query: 242 ANLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTF 301
+++HRWQ VAP++ +LFI T + N + + + +F ++FLG LLS++ + F
Sbjct: 266 TDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRRF 325
Query: 302 PELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKT 361
PELGL + DC E SW++S+ F + V ++ LL P+ + K KSDY EPIS+T
Sbjct: 326 PELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQPV--NYLKRKSDYVREPISRT 385
Query: 362 ALEGMWKRFNDEELETV--------------QLMLIPFGAAYPIQIGYYVTWQRRNDDLK 421
LE +WK+ + E+ T+ + P+ A +I Y W+ +
Sbjct: 386 GLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLTDR 445
Query: 422 HLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYFGNNFE 481
++E TR+LY +MTPFVSK+PR ++ NY+D+D+G N+ +G +SY E +G +YF NFE
Sbjct: 446 YMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFE 505
Query: 482 RLMEVKTKVDPCNFFRHEQSIP 488
RL+++KT+VD NFFR+EQSIP
Sbjct: 506 RLVKIKTRVDSGNFFRNEQSIP 524
BLAST of PI0012249 vs. TAIR 10
Match:
AT5G44440.1 (FAD-binding Berberine family protein )
HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-128
Identity = 233/508 (45.87%), Postives = 328/508 (64.57%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLIHTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFHA 61
EDF+ C+ + N+N +IHT SS+ IL+ SI+N RFS +TPKP++IITP A
Sbjct: 29 EDFLKCLS-YRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 88
Query: 62 SQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVS-NVPFVLIDLIYLNSIAIDVEDESAWV 121
S VQ + C + H + +RTRS GH +EG SY++ N PF +IDL L SI++DV++ + WV
Sbjct: 89 SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 148
Query: 122 QSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDAHL 181
Q+GAT GELYY I K +++L FPAG PT+G+GG SGGG+G ++RKYGL DN++DA +
Sbjct: 149 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 208
Query: 182 VDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQKAA 241
VD +GR++DR +MGED FWAIRGGGG SFG++L+WK+KLV +PS +T F + KT ++A
Sbjct: 209 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 268
Query: 242 NLVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQKTFP 301
++ +WQY A +V DLFI T + SN + + A F L++G N LL+LM++ FP
Sbjct: 269 RIIKKWQYAADKVPDDLFIRTTLERSNKN-----AVHALFTGLYIGPVNNLLALMEEKFP 328
Query: 302 ELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPISKTA 361
ELGL+KE C EMSW+ES+ + + F ++ +L NR S +K K D+ EPI + A
Sbjct: 329 ELGLEKEGCEEMSWIESVLWF-ADFPKGESLGVLTNRERTSLS-FKGKDDFVQEPIPEAA 388
Query: 362 LEGMWKRFNDEELETVQLMLIPFGAAYPIQIGY---------------YVTWQRRNDDL- 421
++ +W+R E +++L PFG Y YV + R +D
Sbjct: 389 IQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKN 448
Query: 422 -----KHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRY 481
K+L+W +Y++MTP+VSKSPR AY+N+KD+D+G T YEE WG +Y
Sbjct: 449 KTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKK-KTKYEEGKSWGVKY 508
Query: 482 FGNNFERLMEVKTKVDPCNFFRHEQSIP 488
F NNFERL+ VKT+VDP +FF EQSIP
Sbjct: 509 FKNNFERLVRVKTRVDPTDFFCDEQSIP 527
BLAST of PI0012249 vs. TAIR 10
Match:
AT5G44400.1 (FAD-binding Berberine family protein )
HSP 1 Score: 451.1 bits (1159), Expect = 1.2e-126
Identity = 232/508 (45.67%), Postives = 327/508 (64.37%), Query Frame = 0
Query: 2 EDFVNCIHVHSSNNNDSNSTLI-HTPSSSSYSYILNFSIRNLRFSEPQTPKPLAIITPFH 61
+ F+NC+ ++ T T + S +S +L + +NLRF + PKP I +P H
Sbjct: 33 DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92
Query: 62 ASQVQASVHCCKSHNLQIRTRSGGHDFEGCSYVSNV--PFVLIDLIYLNSIAIDVEDESA 121
S VQAS+ C K + +R RSGGHD+EG SYVS + PF+L+DL + + I+++D SA
Sbjct: 93 ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152
Query: 122 WVQSGATVGELYYRISKKSQTLGFPAGFAPTIGIGGFLSGGGFGMMVRKYGLGVDNVVDA 181
WVQSGATVGELYYRI++KS+ GFPAG ++GIGG ++GG +G M+RKYGLG DNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212
Query: 182 HLVDGNGRVVDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVQIPSIVTSFALHKTWDQK 241
+VD NG+++DR +MGED FWAIRGG GGSFGI+LAWK+KLV +P VT F + KT Q
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272
Query: 242 AAN-LVHRWQYVAPEVDRDLFISTWVTASNSSEGGGRIMEASFFSLFLGKANKLLSLMQK 301
N ++ +WQ VA ++ +LFI V + + GG + + S+ +LFLG L+++M+K
Sbjct: 273 VGNKIISKWQRVADKLVEELFIR--VLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKK 332
Query: 302 TFPELGLKKEDCLEMSWVESMAFMGSGFVSTNNVKLLLNRTPLSNDRYKTKSDYATEPIS 361
+FPELGL +DC+EMSW+ES+A++ T LL ++P +K KSD+ PI
Sbjct: 333 SFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIP 392
Query: 362 KTALEGMWKRFNDEE------------LETVQLMLIPF----GAAYPIQIGYYVTW-QRR 421
++ L+G++K+ E+ + + IPF G + +Q Y +W
Sbjct: 393 ESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQ--YVTSWLDSD 452
Query: 422 NDDLKHLEWTRELYDYMTPFVSKSPRAAYMNYKDLDIGTNNEDGIPTSYEEASVWGHRYF 481
+H+ W R+LY YMTP+VS +PR AY+NY+DLD+G N +D + T ++A VWG YF
Sbjct: 453 KRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANYF 512
Query: 482 GNNFERLMEVKTKVDPCNFFRHEQSIPP 489
NNF RLM +K KVDP NFFRHEQSIPP
Sbjct: 513 KNNFNRLMMIKAKVDPENFFRHEQSIPP 535
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q8GTB6 | 2.4e-130 | 45.01 | Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... | [more] |
| Q9FI25 | 4.0e-130 | 46.38 | Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... | [more] |
| Q33DQ2 | 5.8e-129 | 43.64 | Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1 | [more] |
| A6P6W0 | 1.7e-128 | 43.84 | Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=... | [more] |
| A6P6W1 | 1.7e-128 | 43.64 | Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5D3D0K7 | 2.1e-238 | 80.24 | Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... | [more] |
| A0A1S3CIZ3 | 2.1e-238 | 80.24 | tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10350149... | [more] |
| A0A6J1HP64 | 2.8e-219 | 73.77 | berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE... | [more] |
| A0A6J1I038 | 9.1e-218 | 74.06 | berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3... | [more] |
| A0A6J1HYZ7 | 1.5e-217 | 74.26 | berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038894011.1 | 7.8e-248 | 83.20 | berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida] | [more] |
| XP_038894010.1 | 1.9e-246 | 83.04 | berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida] | [more] |
| XP_004147307.2 | 9.2e-241 | 81.23 | berberine bridge enzyme-like 27 [Cucumis sativus] | [more] |
| KAE8652747.1 | 9.2e-241 | 81.23 | hypothetical protein Csa_013703 [Cucumis sativus] | [more] |
| XP_008463307.2 | 4.3e-238 | 80.24 | PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK17150.1 ... | [more] |
