PI0010935 (gene) Melon (PI 482460) v1

Overview
NamePI0010935
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4-like
Locationchr04: 4429878 .. 4431620 (+)
RNA-Seq ExpressionPI0010935
SyntenyPI0010935
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCTCAACCTCAAATCCCACCTCCCCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCTCCGGTGCAACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGCCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAACGTCGGCGTCATCAGCGGTCTCATCAACGAGGTTGCTCCCGCCTGGGTCGTTCTTCTTATCGGTGCCGTCATGAACCTTTTTGGCTACACCATGATCTGGTTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAGACCTTTGCAAACACAGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGTGGCGCCATCTTGTCCCAACTTTTCCGTGCCTTTTATGGCAATAATTCCAAATCTCTTATCCTCTTAATCGCTTGGCTTCCCGCCGCCGTCTCTGTTGTTTTCCTCCGTTTCATTCGCATCATTAAGGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATATCCTTTACATTTCTCTCGGCCTTGCTGGGTCTCTAATGGTTTTGATTATTTTGCAAAATCTTTTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTACTTTTTCTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAACCCAATCTTACAATTAGAGTTAGCATCTCAACAACCAACGCCGCCACTGACTTCGACGGCGCCACTAGCGCCCTCGAGCCCTCCGTCGGACTCCTGCTTTAAAAATATGTTTAAACCTCCAAACAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGGGTCGGTGGAACATTGACGGTGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGCGGTACCCATCTCACAGTACCACCACGTTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTTGCCACGCTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCAACTTTGTACAATTTGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGGGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACAGAAAGGCATTTCTTGTAATCACGGCAGCGACGGTGTTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTTTATAAGGGCGATATTTACCGGAAGTTCAGGGAGGAGGAAGGTGAAGACATGGAAATGAAAATGGCCGCTCCGACCAACAGTACGTTGTCGGCAGCGAAAAACTGA

mRNA sequence

ATGGCCGCCTCAACCTCAAATCCCACCTCCCCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCTCCGGTGCAACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGCCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAACGTCGGCGTCATCAGCGGTCTCATCAACGAGGTTGCTCCCGCCTGGGTCGTTCTTCTTATCGGTGCCGTCATGAACCTTTTTGGCTACACCATGATCTGGTTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAGACCTTTGCAAACACAGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGTGGCGCCATCTTGTCCCAACTTTTCCGTGCCTTTTATGGCAATAATTCCAAATCTCTTATCCTCTTAATCGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATATCCTTTACATTTCTCTCGGCCTTGCTGGGTCTCTAATGGTTTTGATTATTTTGCAAAATCTTTTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTACTTTTTCTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAACCCAATCTTACAATTAGAGTTAGCATCTCAACAACCAACGCCGCCACTGACTTCGACGGCGCCACTAGCGCCCTCGAGCCCTCCGTCGGACTCCTGCTTTAAAAATATGTTTAAACCTCCAAACAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGGGTCGGTGGAACATTGACGGTGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGCGGTACCCATCTCACAGTACCACCACGTTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTTGCCACGCTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCAACTTTGTACAATTTGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGGGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACAGAAAGGCATTTCTTGTAATCACGGCAGCGACGGTGTTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTTTATAAGGGCGATATTTACCGGAAGTTCAGGGAGGAGGAAGGTGAAGACATGGAAATGAAAATGGCCGCTCCGACCAACAGTACGTTGTCGGCAGCGAAAAACTGA

Coding sequence (CDS)

ATGGCCGCCTCAACCTCAAATCCCACCTCCCCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCTCCGGTGCAACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGCCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAACGTCGGCGTCATCAGCGGTCTCATCAACGAGGTTGCTCCCGCCTGGGTCGTTCTTCTTATCGGTGCCGTCATGAACCTTTTTGGCTACACCATGATCTGGTTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAGACCTTTGCAAACACAGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGTGGCGCCATCTTGTCCCAACTTTTCCGTGCCTTTTATGGCAATAATTCCAAATCTCTTATCCTCTTAATCGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATATCCTTTACATTTCTCTCGGCCTTGCTGGGTCTCTAATGGTTTTGATTATTTTGCAAAATCTTTTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTACTTTTTCTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAACCCAATCTTACAATTAGAGTTAGCATCTCAACAACCAACGCCGCCACTGACTTCGACGGCGCCACTAGCGCCCTCGAGCCCTCCGTCGGACTCCTGCTTTAAAAATATGTTTAAACCTCCAAACAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGGGTCGGTGGAACATTGACGGTGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGCGGTACCCATCTCACAGTACCACCACGTTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTTGCCACGCTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCAACTTTGTACAATTTGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGGGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACAGAAAGGCATTTCTTGTAATCACGGCAGCGACGGTGTTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTTTATAAGGGCGATATTTACCGGAAGTTCAGGGAGGAGGAAGGTGAAGACATGGAAATGAAAATGGCCGCTCCGACCAACAGTACGTTGTCGGCAGCGAAAAACTGA

Protein sequence

MAASTSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSLILLIDLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAKN
Homology
BLAST of PI0010935 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.1e-27
Identity = 134/549 (24.41%), Postives = 247/549 (44.99%), Query Frame = 0

Query: 19  LFGRWFS---VFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGV 78
           +FGRW     + A+I I + +G  + F+ YSS +KS L   Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 79  ISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYIC--IGANSQTF 138
            SG+     P  VVL   A M   GY + WL +TN I  P   +  +++C  +   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICW 156

Query: 139 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSLILLIDLRQPN 198
            NT   + C+++FP +R   L L   F G+S A+ S  F A    +S +L LL++   P 
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI-NPSSSNLYLLLNSLVP- 216

Query: 199 ELKVFYHILYISLGL---------------AGSLMVLIILQNLFRFQ-----------QI 258
            L V +  LY  L                 +    +L +L  +  F            ++
Sbjct: 217 -LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARL 276

Query: 259 QYVGSAIVVIVLLFLPLAIVFREE-LSVWKSKIANP-----ILQLELASQQPTPPLTSTA 318
            ++G+ ++++  L  PL +  R+  L V  +++ +      +L ++    Q T   + T 
Sbjct: 277 NFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG 336

Query: 319 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQ 378
                +    +  +        G++++    I  ++  + +IA  CG    L   +NLGQ
Sbjct: 337 YEHMGTAKEGNTVR-------LGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQ 396

Query: 379 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 438
           I +SL     ++TT +++ S +++ GR++S    ++  K++++ R  +    L+ + +  
Sbjct: 397 IAQSL---GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAF 456

Query: 439 LLIAF--GVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAY 498
            L+A       +L  ++ +IG   G  +    +I S++FG       +N+     PIG+ 
Sbjct: 457 FLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS- 516

Query: 499 ILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLI 529
           +L   +A  +Y+  A   +        + + + C+G +CY K F+     ++ G + SL 
Sbjct: 517 LLYGYIAASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLS 562

BLAST of PI0010935 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.6e-297
Identity = 531/580 (91.55%), Postives = 544/580 (93.79%), Query Frame = 0

Query: 1   MAASTSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTL 60
           MAASTSNPTS NPFWYQFLFGRWFSVFASILIMSV+GATYMFALYSSDIKSSL YDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNS 180
           CLYIC+GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLF AFYGN+S
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 KSLILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNL 240
           KSLILLI                   DLRQPNE+ VFYHILYISL LAG+LMVLIILQ+L
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 FRFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTA 300
            RFQQIQYVGSAIVVIVLL LPLAIVFREELSVWKSKIANPILQLELASQQP PPLTST 
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQ 360
           PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLT IDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYIL 480
           LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GGAASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFL+ITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAKN 562
           VRTWKFYKGDIYR+FR EEGED+EMKMAAPTNSTL+ AKN
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMKMAAPTNSTLTTAKN 580

BLAST of PI0010935 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 6.9e-288
Identity = 516/580 (88.97%), Postives = 536/580 (92.41%), Query Frame = 0

Query: 1   MAASTSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTL 60
           MAAS+SNPTS NPFWYQFLFGRWFSV ASILIMSV+GATYMFALYSSDIKSSL YDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNS 180
           CLYI IGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+RAFYGNN 
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 KSLILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNL 240
           +SLILLI                   DLRQPNELKVFYH LYISLGLAG+LMVLIILQ+L
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 FRFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTA 300
            RFQQIQYVGSAIVVIVLL LPL IVFREELSVWKSKIA+P+LQLE ASQQP PPLTST 
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLT IDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYIL 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFL+ITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAKN 562
           VRTWKFYKGDIYRKFRE EGED+EMKMAAPTN TL+AAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

BLAST of PI0010935 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.8e-273
Identity = 485/576 (84.20%), Postives = 519/576 (90.10%), Query Frame = 0

Query: 4   STSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSVSGATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSL 183
           ICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+ AFYGNNSKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 243
           ILLI                   DLRQPNEL+VF+HILYISLGLAGSLMVLIILQN  +F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 303
            Q++Y GSAIVVI LL LPLAIV+REELSVWK+KI +P+ QLELASQQ  PPLTS AP+A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTIPQA+FSIDMIILFIATICGVGGTLT IDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGG ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFL+ITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           WKFYKGDIYRKFREE GE++E++MAAPTNS ++ ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

BLAST of PI0010935 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 9.0e-272
Identity = 485/571 (84.94%), Postives = 509/571 (89.14%), Query Frame = 0

Query: 9   TSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKD 68
           TS NPFWY  LFGRWFSVFASILIMSVSGATYMF LYSS IKSSLAYDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 69  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGA 128
           LG N+GVISGL+NEVAPAWVVLLIG VMNLFGYTMIWLAVTNRIP PQIWHMCLYICIGA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSLILLI- 188
           NSQTFANT AL+TCVKNFPESRGS+LGL KGFVGLSGAILSQLF AFYGNNSKSLI LI 
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 189 ------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRFQQIQY 248
                             DLRQPNELKVFYH+LYISLGLAGSLMV IILQN  RFQQI Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 249 VGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLAPSSPP 308
           VGSAIVVIVLL LPLAIVFREEL +W+SKI NPI QLELASQ P PP        P +PP
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP--------PPNPP 302

Query: 309 SDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGESLRYP 368
           SDSCFKNMF PPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLT IDNLGQIGESLRYP
Sbjct: 303 SDSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 362

Query: 369 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVP 428
           SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GHLLIAFGVP
Sbjct: 363 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 422

Query: 429 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAGHL 488
           NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GGAASPIGAYI+NVRVAGHL
Sbjct: 423 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 482

Query: 489 YDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRTWKFYK 548
           YD EA+RQMEA GR R  GEDLSCLGVECY+KAFL+IT +TV G LVSLILVVRTWKFYK
Sbjct: 483 YDMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYK 542

Query: 549 GDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
            DIYR+F+E+EGED+E+K+AAPTN++L+AAK
Sbjct: 543 DDIYRRFKEKEGEDIEIKIAAPTNNSLTAAK 565

BLAST of PI0010935 vs. ExPASy TrEMBL
Match: A0A6J1KSH4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498302 PE=4 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.9e-271
Identity = 482/576 (83.68%), Postives = 517/576 (89.76%), Query Frame = 0

Query: 4   STSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSVSGATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSL 183
           ICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+ AFYGNNSKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 243
           ILLI                   DLRQPNEL+VF+HILYISLGLAGSLMVLIILQN  +F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 303
            Q++Y GSAIVVI LL LPLAIV+REELSVWK+KI +PI QLELASQ   PPLTS AP+A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPVA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTI QA+FSIDMIILFIATICGVGGTLT IDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGG ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFL+ITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           WKFYKGDIY+KFRE+ GE++E++MAAPTNS ++ ++
Sbjct: 542 WKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQ 577

BLAST of PI0010935 vs. NCBI nr
Match: KAA0043983.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.4e-297
Identity = 531/580 (91.55%), Postives = 544/580 (93.79%), Query Frame = 0

Query: 1   MAASTSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTL 60
           MAASTSNPTS NPFWYQFLFGRWFSVFASILIMSV+GATYMFALYSSDIKSSL YDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNS 180
           CLYIC+GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLF AFYGN+S
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 KSLILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNL 240
           KSLILLI                   DLRQPNE+ VFYHILYISL LAG+LMVLIILQ+L
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 FRFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTA 300
            RFQQIQYVGSAIVVIVLL LPLAIVFREELSVWKSKIANPILQLELASQQP PPLTST 
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQ 360
           PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLT IDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYIL 480
           LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GGAASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFL+ITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAKN 562
           VRTWKFYKGDIYR+FR EEGED+EMKMAAPTNSTL+ AKN
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMKMAAPTNSTLTTAKN 580

BLAST of PI0010935 vs. NCBI nr
Match: XP_011651970.1 (uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical protein Csa_001993 [Cucumis sativus])

HSP 1 Score: 999.2 bits (2582), Expect = 1.4e-287
Identity = 516/580 (88.97%), Postives = 536/580 (92.41%), Query Frame = 0

Query: 1   MAASTSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTL 60
           MAAS+SNPTS NPFWYQFLFGRWFSV ASILIMSV+GATYMFALYSSDIKSSL YDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNS 180
           CLYI IGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+RAFYGNN 
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 KSLILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNL 240
           +SLILLI                   DLRQPNELKVFYH LYISLGLAG+LMVLIILQ+L
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 FRFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTA 300
            RFQQIQYVGSAIVVIVLL LPL IVFREELSVWKSKIA+P+LQLE ASQQP PPLTST 
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLT IDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYIL 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFL+ITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAKN 562
           VRTWKFYKGDIYRKFRE EGED+EMKMAAPTN TL+AAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

BLAST of PI0010935 vs. NCBI nr
Match: XP_038905012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 996.5 bits (2575), Expect = 9.2e-287
Identity = 516/576 (89.58%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 4   STSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLL 63
           + SNPTS NPFWYQFLFGRWFSVFASILIMSV+GATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 2   AASNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 121

Query: 124 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSL 183
           ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLF AFYGNNSKSL
Sbjct: 122 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNNSKSL 181

Query: 184 ILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 243
           I LI                   DLRQPNELKVFYHILYISLGLAGSLMVLIILQN  RF
Sbjct: 182 IFLIAWLPAAVSVTFLRFVRIIKDLRQPNELKVFYHILYISLGLAGSLMVLIILQNRLRF 241

Query: 244 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 303
           QQIQYVGSAIVVIVLL LPLAIVFREEL+VWKSKIANPILQLELASQQP  P     PLA
Sbjct: 242 QQIQYVGSAIVVIVLLLLPLAIVFREELNVWKSKIANPILQLELASQQPPRP----PPLA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 363
           P SPPS SCFKN FKPPNRGEDYTIPQAIFS+DMIILFIATICGVGGTLT IDNLGQIGE
Sbjct: 302 PPSPPSGSCFKNTFKPPNRGEDYTIPQAIFSVDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SL YPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI
Sbjct: 362 SLGYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 483
           AFGVPNSLYFSSI++GFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIVVGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 543
           VAGHLYDREAQRQMEA GRRRNIGEDLSCLGVECYRKAFL+ITAATVFG LVSLILVVRT
Sbjct: 482 VAGHLYDREAQRQMEAAGRRRNIGEDLSCLGVECYRKAFLIITAATVFGGLVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           WKFYK DIYRKFREEE ED+E+K+  P N  L+AAK
Sbjct: 542 WKFYKSDIYRKFREEEAEDVEIKIVTPANGALTAAK 573

BLAST of PI0010935 vs. NCBI nr
Match: KAG6596491.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 950.7 bits (2456), Expect = 5.8e-273
Identity = 485/576 (84.20%), Postives = 519/576 (90.10%), Query Frame = 0

Query: 4   STSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSVSGATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSL 183
           ICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+ AFYGNNSKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 243
           ILLI                   DLRQPNEL+VF+HILYISLGLAGSLMVLIILQN  +F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 303
            Q++Y GSAIVVI LL LPLAIV+REELSVWK+KI +P+ QLELASQQ  PPLTS APLA
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPLA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTIPQA+FSIDMIILFIATICGVGGTLT IDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGG ASPIGAYILNV+
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVK 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFL+ITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           WKFYKGDIYRKFREE GE++E++MAAPTNS ++ ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

BLAST of PI0010935 vs. NCBI nr
Match: XP_022947808.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 950.7 bits (2456), Expect = 5.8e-273
Identity = 485/576 (84.20%), Postives = 519/576 (90.10%), Query Frame = 0

Query: 4   STSNPTSPNPFWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSVSGATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSL 183
           ICIGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+ AFYGNNSKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLI-------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 243
           ILLI                   DLRQPNEL+VF+HILYISLGLAGSLMVLIILQN  +F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 303
            Q++Y GSAIVVI LL LPLAIV+REELSVWK+KI +P+ QLELASQQ  PPLTS AP+A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTIPQA+FSIDMIILFIATICGVGGTLT IDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGG ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFL+ITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           WKFYKGDIYRKFREE GE++E++MAAPTNS ++ ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

BLAST of PI0010935 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 674.5 bits (1739), Expect = 7.5e-194
Identity = 355/578 (61.42%), Postives = 424/578 (73.36%), Query Frame = 0

Query: 17  QFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVI 76
           Q L GRWF  F S+LIMS +GATYMF +YS DIK +L YDQTTLNLLSFFKDLG NVGV+
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 77  SGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGANSQTFANT 136
           +GL+NEV P W +LLIGA++N FGY MIWLAVT RI KPQ+WHMCLYIC+GANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 137 GALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSLILLID-------- 196
           G+LVTCVKNFPESRG VLG+LKG+VGLSGAI++QL+RAFYG ++K LIL+I         
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 197 -----------LRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRFQQIQYVGSAIVVI 256
                       RQ NELKVFY+ LYISLGLA  LMV+II+  L  F Q ++ GSA VVI
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 257 VLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLAPSSPPSD------ 316
           VLL LP+ +V  EE  +WK K      Q+ L    P   +T    L  S    D      
Sbjct: 256 VLLLLPIIVVILEEKKLWKEK------QVALNDPAPINVVTEKPKLDSSEFKDDDGEESK 315

Query: 317 ---------SCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQI 376
                    SC+  +F PP RG+DYTI QA+FS+DM+ILF+ATICGVGGTLT IDNLGQI
Sbjct: 316 EVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQI 375

Query: 377 GESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHL 436
           G SL YP  S +TF+SLVSIWNY GRVVSG VSE F  KYK PRPL L   L+LSC GHL
Sbjct: 376 GNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHL 435

Query: 437 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILN 496
           LIAF VP  LY +S+IIGFCFGAQWPL+FAIISEIFGLKYY+TLYN G  ASPIG+Y+LN
Sbjct: 436 LIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLN 495

Query: 497 VRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVV 556
           VRVAG+LYD EA +Q +A G+ R  G+DL+C+G  C++ +F++I A T+FG LVS++LV+
Sbjct: 496 VRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVI 555

Query: 557 RTWKFYKGDIYRKFREEEGEDMEMKMAAPTNSTLSAAK 561
           RT KFYK DIY+KFR E+    EM+MAAP  +  + AK
Sbjct: 556 RTKKFYKSDIYKKFR-EKALAAEMEMAAPAAARSTVAK 586

BLAST of PI0010935 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 612.1 bits (1577), Expect = 4.6e-175
Identity = 324/556 (58.27%), Postives = 395/556 (71.04%), Query Frame = 0

Query: 14  FWYQFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNV 73
           F   F  GRWF VFAS LIM+ +GATY+F  YS DIKS+L YDQTTLNLL FFKDLG NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 74  GVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGANSQTF 133
           GV+SGLI EV P W VL IG+ MN  GY MIWL VT ++ KP++W MCLYICIGANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKSLILLID----- 193
           ANTGALVTCVKNFPESRG +LGLLKG+VGLSGAI +QL+ A YG++SKSLILLI      
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 194 --------------LRQPNELKVFYHILYISLGLAGSLMVLIILQNLFRFQQIQYVGSAI 253
                         +RQ NEL VFY  LYIS+ LA  LM + I +    F +  Y  SA 
Sbjct: 189 VSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASAT 248

Query: 254 VVIVLLFLPLAIVFREELSVW---KSKIANPI-LQLELASQQPTPPLTSTAPLAPSSPPS 313
           +   LLF+PL +  ++EL VW   K  I  P  +++E   ++        A +      +
Sbjct: 249 ICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKET 308

Query: 314 DSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGESLRYPS 373
            SCF  +F PP RGEDYTI QA+ S DMIILF+AT CG+G +LT +DNLGQIGESL YP+
Sbjct: 309 KSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPN 368

Query: 374 HSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 433
           H+ ++F+SLVSIWNY GRV SGFVSEY   KYK+PRPL +   L+LSC GHLLIAF VP 
Sbjct: 369 HTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPG 428

Query: 434 SLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAGHLY 493
           S+Y +SI++GF FGAQ PL+FAIISE+FGLKYY+TL+N G  ASP+G+YILNVRV G LY
Sbjct: 429 SVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLY 488

Query: 494 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRTWKFYKG 547
           D+EA +Q+ A G  R   +DL+CLG +CY+  FL++ A T FGALVSL L +RT +FYKG
Sbjct: 489 DKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKG 548

BLAST of PI0010935 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 3.0e-86
Identity = 197/544 (36.21%), Postives = 304/544 (55.88%), Query Frame = 0

Query: 22  RWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVISGLIN 81
           +W +  ASI I S SGATY FA+YSS +KSS +YDQ+TL+ +S FKD+GG  G+ISG + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 82  EVAPA--------WVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICIGANSQTF 141
               +        WVV+ +G V    G+  IW +V   I  P +  MCL++ +  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 142 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYG--NNSKSLILLID--- 201
            NT  +VT  +NF +  G+ +G+++GF+GLSGAIL QL+ A  G   N  + ILL+    
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 202 -----LRQP----------NELKVFYHILYISLGLAGSLMVLIILQNLFRFQQIQYVGSA 261
                L  P          ++ K    +  IS+ +A  LMV+I ++N+    +   + S 
Sbjct: 187 TLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFSF 246

Query: 262 IVVIVLLFLPLAIVFR--EELSVWKSKIANPILQLELASQQPTPPLTSTAPLAPSSPPSD 321
           I+V++LL  PL +  R   E     S +  P+L                   A   PPS 
Sbjct: 247 ILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTS----------------ALLDPPSS 306

Query: 322 SCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGESLRYPSH 381
           + F +        ED  I +A+ +++  +LF+A +CG+G     ++N+ QIGESLRY S 
Sbjct: 307 NIFPD--GDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSV 366

Query: 382 STTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPNS 441
              + +SL SIWN+LGR  +G+VS+ F  K+  PRP+F+  TL +  +GH+++A GV  S
Sbjct: 367 QLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGS 426

Query: 442 LYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVRVAGHLYD 501
           LY  S++IG  +G+QW L+  I SEIFG+++  T+Y     A PIG+YIL+V+V G+ YD
Sbjct: 427 LYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYD 486

Query: 502 REAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRTWKFYKGD 536
           + A              +D SC G +C+R +F+++ +  +FG+LV+ +L  RT KFYK  
Sbjct: 487 KVASE------------DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNL 520

BLAST of PI0010935 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-85
Identity = 193/547 (35.28%), Postives = 301/547 (55.03%), Query Frame = 0

Query: 17  QFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVI 76
           + L  +W ++ ASI I   SGA+Y F +YS+ +KS+ +YDQ+TL+ +S FKD+G N GV 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 77  SGLINEVAPA----------------WVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 136
           SGL+   A +                WVVL +GA+    GY +IW +VT  I KP +  M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 137 CLYICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNS 196
           CL++ + A SQTF NT  +V+ V+NF +  G+ +G++KGF+GLSGAIL QL+      + 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 197 KSLILLI------------------DLRQPNELKVFYHILYISLGLAGSLMVLIILQNLF 256
            S ILL+                  +    ++ K    +  +SL +A  LM++IIL+N F
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTF 241

Query: 257 RFQQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAP 316
                  + + + ++V+L LPL I  R +    +  + +            +P ++S   
Sbjct: 242 GLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDY----------SPLISSPKA 301

Query: 317 LAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQI 376
               +  S+   K         E+  + QA+  +   +LF+A ICG+G  L+ I+N+ QI
Sbjct: 302 TTSGNQSSEGDSK---VEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQI 361

Query: 377 GESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHL 436
           GESLRY S    + +SL SIWN+LGR  +G+ S+    K   PRPL + ATL    +GHL
Sbjct: 362 GESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHL 421

Query: 437 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILN 496
           +IA G   +LY  S+I+G C+G+QW L+  I SE+FG+++  T++N    ASPIG+YI +
Sbjct: 422 IIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFS 481

Query: 497 VRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVV 530
           VR+ G++YD+ A             GE  +C G  C+R +F+++ +   FG LV+++L  
Sbjct: 482 VRLIGYIYDKTAS------------GEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFF 523

BLAST of PI0010935 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 8.3e-84
Identity = 194/545 (35.60%), Postives = 301/545 (55.23%), Query Frame = 0

Query: 17  QFLFGRWFSVFASILIMSVSGATYMFALYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGVI 76
           + L  +W ++ ASI I   +G +Y F +YS+ +KS+ +YDQ+TL+ +S FKD+GGNVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 77  SGLINEVAP--------------AWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCL 136
           SGL+   A                WVV+LIGA++N  GY ++W +VT  I +P +  MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 137 YICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFRAFYGNNSKS 196
           ++ I A S TF NT  +V+ ++NF +  G+ +G++KGFVGLSGA+L QL+      + K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 197 LILLIDL------------------RQPNELKVFYHILYISLGLAGSLMVLIILQNLFRF 256
            ILL+ +                     +E K    +  +SL +A  LM+ IIL++    
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSL 243

Query: 257 QQIQYVGSAIVVIVLLFLPLAIVFREELSVWKSKIANPILQLELASQQPTPPLTSTAPLA 316
                  +  V++VLL  PL +  R      +  I  P+  +              +PL 
Sbjct: 244 PSWANAVTLAVLLVLLSSPLLVAVRAH----RDSIEKPLSSV-------------YSPLV 303

Query: 317 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTVIDNLGQIGE 376
            +   + S    M    +  +   + QA+ ++D  +LF+A ICG+G  ++ I+N+ QIGE
Sbjct: 304 DNLEATTSGEILML---DEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGE 363

Query: 377 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 436
           SLRY S    + ++L +IWN++GR   G+VS++   +   PRPL +  TL    +GHL+I
Sbjct: 364 SLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLII 423

Query: 437 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 496
           A G   +LY  SII+G C+G+QW L+  I SE+FG+K+  T+YN    ASP+G+YI +VR
Sbjct: 424 ASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVR 483

Query: 497 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLVITAATVFGALVSLILVVRT 530
           + G++YDR              IGE  +C G  C+R A++VI +    G LVS +LV RT
Sbjct: 484 LIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRT 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E13.1e-2724.41Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TKH41.6e-29791.55Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0LEA46.9e-28888.97Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G74260... [more]
A0A6J1G7M82.8e-27384.20protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7TKY59.0e-27284.94Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1KSH45.9e-27183.68protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498... [more]
Match NameE-valueIdentityDescription
KAA0043983.13.4e-29791.55protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 p... [more]
XP_011651970.11.4e-28788.97uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical ... [more]
XP_038905012.19.2e-28789.58protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
KAG6596491.15.8e-27384.20Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022947808.15.8e-27384.20protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G39210.17.5e-19461.42Major facilitator superfamily protein [more]
AT2G28120.14.6e-17558.27Major facilitator superfamily protein [more]
AT2G34355.13.0e-8636.21Major facilitator superfamily protein [more]
AT1G74780.13.4e-8535.28Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.18.3e-8435.60Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 190..248
e-value: 1.7E-6
score: 27.7
coord: 22..189
e-value: 2.9E-74
score: 249.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 286..527
e-value: 2.2E-13
score: 52.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 9..179
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 202..526
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 12..554
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 12..554
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 21..514
e-value: 3.82918E-114
score: 342.694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010935.1PI0010935.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099587 inorganic ion import across plasma membrane
biological_process GO:0002239 response to oomycetes
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity