PI0009499 (gene) Melon (PI 482460) v1

Overview
NamePI0009499
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionElongation factor Tu
Locationchr02: 24624561 .. 24625994 (+)
RNA-Seq ExpressionPI0009499
SyntenyPI0009499
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCATTTCCTTAGCTTCCGTTTCCACTTCTTCTAAGCTTGTTTTCCCCCACCCTTCTTCTTCTTCTTCTACTACTTCTTCTTCTTCCCTTTCTTCTTCCCTCCTCTCCAAACCCTCTTCCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCAATTCGTCCTCTCTCCTTCTCTTCCCCTTCTGTAAACCGTCCTCGTTCCTTCACAATTCGTGCCGCTCGTGGGAAATTTGAGAGGAAGAAACCCCATGTCAATATTGGCACAATTGGCCATGTCGACCATGGTAAAACCACTCTCACTGCTGCTTTAACCATGGCTTTAAGCAAAGCCCAAAGAAATACGATGAAATTGACGCTGCCCCAGAAGAACGAGCTCGTGGTATTACGATTAATACAGCTACTGTTGAGTACGAGACTGAGAGTCGACATTATGCTCATGTTGATTGTCCTGGACATGCTGATTATGTCAAGAATATGATTACTGGTGCTGCGCAAATGGATGGAGCGATTTTGGTTGTTTCTGGTGCTGATGGTCCAATGCCACAGACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTTCCTAATATGGTGGTGTTTTTGAACAAGAAGGATCAAGTTGATGATGAGGAGCTTTTGGAGCTTGTGGAGTTGGAGATGCGTGAGTTGCTTTCTTCTTATGAATTCCCAGGTGATGATGTACCAATTATTGCTGGTTCTGCTTTGTTAGCTTTGGAAGCTTTAATGGCTAACAATAATATAGCTAGAGGTGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATGCTGTTGATAGTTATATACCAATACCTGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTGTTCTCTATTACTGGTCGTGGTACTGTTGCTACAGGGCGTGTTGAAAGAGGAACCGTTAGAGTGGGAGAGACAGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACTGTAACAGGGGTGGAAATGTTTCAAAAAATTCTTGATGAGGCTTTGGCTGGGGATAATGTTGGTTGTTGCTTCGAGGTGTTCAGAAGGCTGATATCCAAAGAGGGATGGTTTTAGCCAAGCCTGGCACTATCACCCACATACCAAGTTTAGTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCTGGTTACAGACCGCAATTCTATATGAGGACGACTGATGTCACTGGCAAGGTGTCTTCAATTATGAATGACAAGGACGAGGAGTCGAAAATGGTTATGCCGGGAGACCGAGTGAAAATGGTTGTGGAACTGATTATGCCTGTGGCTTGTGAACAAGGAATGAGGTTTGCTATCAGAGAAGGTGGAAAGACTGTTGGTGCTGGTGTGATTCAATCAATTATCGAGTGA

mRNA sequence

ATGGCGGCCATTTCCTTAGCTTCCGTTTCCACTTCTTCTAAGCTTGTTTTCCCCCACCCTTCTTCTTCTTCTTCTACTACTTCTTCTTCTTCCCTTTCTTCTTCCCTCCTCTCCAAACCCTCTTCCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCAATTCGTCCTCTCTCCTTCTCTTCCCCTTCTGTAAACCGTCCTCGTTCCTTCACAATTCGTGCCGCTCGTGGGAAATTTGAGAGGAAGAAACCCCATGTCAATATTGGCACAATTGGCCATCAAAGCCCAAAGAAATACGATGAAATTGACGCTGCCCCAGAAGAACGAGCTCGTGGTATTACGATTAATACAGCTACTGTTGAGTACGAGACTGAGAGTCGACATTATGCTCATGTTGATTGTCCTGGACATGCTGATTATGTCAAGAATATGATTACTGGTGCTGCGCAAATGGATGGAGCGATTTTGGTTGTTTCTGGTGCTGATGGTCCAATGCCACAGACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTTCCTAATATGGTGGTGTTTTTGAACAAGAAGGATCAAGTTGATGATGAGGAGCTTTTGGAGCTTGTGGAGTTGGAGATGCGTGAGTTGCTTTCTTCTTATGAATTCCCAGGTGATGATGTACCAATTATTGCTGGTTCTGCTTTGTTAGCTTTGGAAGCTTTAATGGCTAACAATAATATAGCTAGAGGTGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATGCTGTTGATAGTTATATACCAATACCTGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTGTTCTCTATTACTGGTCGTGGTACTGTTGCTACAGGGCGTGTTGAAAGAGGAACCGTTAGAGTGGGAGAGACAGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACTGTAACAGGGGTGGAAATGTTTCAAAAAATTCTTGATGAGGCTTTGGCTGGGGATAATGTTGGTTGTTGCTTCGAGGTCCAAGCCTGGCACTATCACCCACATACCAAGTTTAGTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCTGGTTACAGACCGCAATTCTATATGAGGACGACTGATGTCACTGGCAAGGTGTCTTCAATTATGAATGACAAGGACGAGGAGTCGAAAATGGTTATGCCGGGAGACCGAGTGAAAATGGTTGTGGAACTGATTATGCCTGTGGCTTGTGAACAAGGAATGAGGTTTGCTATCAGAGAAGGTGGAAAGACTGTTGGTGCTGGTGTGATTCAATCAATTATCGAGTGA

Coding sequence (CDS)

ATGGCGGCCATTTCCTTAGCTTCCGTTTCCACTTCTTCTAAGCTTGTTTTCCCCCACCCTTCTTCTTCTTCTTCTACTACTTCTTCTTCTTCCCTTTCTTCTTCCCTCCTCTCCAAACCCTCTTCCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCAATTCGTCCTCTCTCCTTCTCTTCCCCTTCTGTAAACCGTCCTCGTTCCTTCACAATTCGTGCCGCTCGTGGGAAATTTGAGAGGAAGAAACCCCATGTCAATATTGGCACAATTGGCCATCAAAGCCCAAAGAAATACGATGAAATTGACGCTGCCCCAGAAGAACGAGCTCGTGGTATTACGATTAATACAGCTACTGTTGAGTACGAGACTGAGAGTCGACATTATGCTCATGTTGATTGTCCTGGACATGCTGATTATGTCAAGAATATGATTACTGGTGCTGCGCAAATGGATGGAGCGATTTTGGTTGTTTCTGGTGCTGATGGTCCAATGCCACAGACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTTCCTAATATGGTGGTGTTTTTGAACAAGAAGGATCAAGTTGATGATGAGGAGCTTTTGGAGCTTGTGGAGTTGGAGATGCGTGAGTTGCTTTCTTCTTATGAATTCCCAGGTGATGATGTACCAATTATTGCTGGTTCTGCTTTGTTAGCTTTGGAAGCTTTAATGGCTAACAATAATATAGCTAGAGGTGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATGCTGTTGATAGTTATATACCAATACCTGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTGTTCTCTATTACTGGTCGTGGTACTGTTGCTACAGGGCGTGTTGAAAGAGGAACCGTTAGAGTGGGAGAGACAGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACTGTAACAGGGGTGGAAATGTTTCAAAAAATTCTTGATGAGGCTTTGGCTGGGGATAATGTTGGTTGTTGCTTCGAGGTCCAAGCCTGGCACTATCACCCACATACCAAGTTTAGTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCTGGTTACAGACCGCAATTCTATATGAGGACGACTGATGTCACTGGCAAGGTGTCTTCAATTATGAATGACAAGGACGAGGAGTCGAAAATGGTTATGCCGGGAGACCGAGTGAAAATGGTTGTGGAACTGATTATGCCTGTGGCTTGTGAACAAGGAATGAGGTTTGCTATCAGAGAAGGTGGAAAGACTGTTGGTGCTGGTGTGATTCAATCAATTATCGAGTGA

Protein sequence

MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGHQSPKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCFEVQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Homology
BLAST of PI0009499 vs. ExPASy Swiss-Prot
Match: Q43467 (Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFA PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 3.4e-194
Identity = 365/480 (76.04%), Postives = 405/480 (84.38%), Query Frame = 0

Query: 4   ISLASVSTSSKLV-FPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFSSP 63
           ++++S + SSKL+  PH SSSSS  S+   SS+  +   + LSSSFL+P+++   + SS 
Sbjct: 1   MAVSSATASSKLILLPHASSSSSLNSTPFRSSTTNTHKLTPLSSSFLHPTTVLRRTPSST 60

Query: 64  SVNRPRSFTIRAARGKFERKKPHVNIGTIGH---------------------QSPKKYDE 123
           +  R R+FT+RAARGKFERKKPHVNIGTIGH                      +PKKYDE
Sbjct: 61  TTPR-RTFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALAALGNSAPKKYDE 120

Query: 124 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 183
           IDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 184 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 243
           DGPMPQTKEHI+LAKQVGVPNMVVFLNK+DQVDDEELL+LVE+E+R+LLSSYEFPGDD P
Sbjct: 181 DGPMPQTKEHIILAKQVGVPNMVVFLNKQDQVDDEELLQLVEIEVRDLLSSYEFPGDDTP 240

Query: 244 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 303
           I++GSALLALEALMAN  I RG+NEWVDKIF+LMD VD+YIPIP+RQTDLPFLLAVEDVF
Sbjct: 241 IVSGSALLALEALMANPAIKRGDNEWVDKIFQLMDEVDNYIPIPQRQTDLPFLLAVEDVF 300

Query: 304 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 363
           SITGRGTVATGRVERGT++VGETVD+VGLRETRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 301 SITGRGTVATGRVERGTIKVGETVDLVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 364 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 423
                          +     PHTKFSA+VYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKTDIQRGMVLAKPGTITPHTKFSAIVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 424 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKV+SIMNDKDEES MV+PGDRVKMVVELI+PVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVTSIMNDKDEESTMVLPGDRVKMVVELIVPVACEQGMRFAIREGGKTVGAGVIQSIIE 479

BLAST of PI0009499 vs. ExPASy Swiss-Prot
Match: P46280 (Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFB1 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.6e-191
Identity = 360/480 (75.00%), Postives = 404/480 (84.17%), Query Frame = 0

Query: 3   AISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFSSP 62
           AIS A+ +T+SKL +P     S + SS+ L        +++LSSSF++P++I  L+ ++ 
Sbjct: 2   AISWAA-ATTSKLAYPPHVHFSPSPSSNYLFLKTHKPSATHLSSSFIHPTTILHLAAANT 61

Query: 63  SVNRPRSFTIRAARGKFERKKPHVNIGTIGH---------------------QSPKKYDE 122
           +  R RSFT+RAARGKFERKKPHVNIGTIGH                      +PKKYDE
Sbjct: 62  TTRR-RSFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASLGNSAPKKYDE 121

Query: 123 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 182
           IDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 122 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 181

Query: 183 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 242
           DGPMPQTKEHILLAKQVGVPN+VVFLNK+DQVDDEELL+LVELE+RELLS YEFPGDDVP
Sbjct: 182 DGPMPQTKEHILLAKQVGVPNIVVFLNKQDQVDDEELLQLVELEVRELLSKYEFPGDDVP 241

Query: 243 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 302
           II+GSALL+LEALMAN +I RGEN+WVDKI+ELM+AVD YIPIP+RQT+LPFLLA+EDVF
Sbjct: 242 IISGSALLSLEALMANPSIKRGENQWVDKIYELMEAVDDYIPIPQRQTELPFLLAIEDVF 301

Query: 303 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 362
           +ITGRGTVATGRVERGT+RVGETVDIVG+++TRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 302 TITGRGTVATGRVERGTIRVGETVDIVGVKDTRNTTVTGVEMFQKILDEALAGDNVGLLL 361

Query: 363 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 422
                          +     PHTKFSA+VYVLKKEEGGRHSPFF+GYRPQFYMRTTDVT
Sbjct: 362 RGIQKTDIQRGMVLAKPGTITPHTKFSAIVYVLKKEEGGRHSPFFSGYRPQFYMRTTDVT 421

Query: 423 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKV+ IMNDKDEESKMVMPGDRVK+VVELI+PVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 422 GKVTEIMNDKDEESKMVMPGDRVKLVVELIVPVACEQGMRFAIREGGKTVGAGVIQSIIE 479

BLAST of PI0009499 vs. ExPASy Swiss-Prot
Match: Q43364 (Elongation factor TuB, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFB PE=2 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 1.9e-189
Identity = 364/487 (74.74%), Postives = 405/487 (83.16%), Query Frame = 0

Query: 1   MAAISLAS--VSTSSKLVFPH-PSSSSSTTSSSSLSSSLLSKPSSNLSSSFL-NPSSIRP 60
           MA+IS AS   + S+KL +P+ PSSSSS+++++++  S  SK    LSSSF   PS++  
Sbjct: 1   MASISAASATATASTKLAYPYSPSSSSSSSNTAAVFPSNSSK--LILSSSFTPTPSTLFL 60

Query: 61  LS-FSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH---------------------Q 120
            S  ++PS   PR FT+RAARGKFERKKPHVNIGTIGH                      
Sbjct: 61  HSPTTTPSTTHPRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASMGNS 120

Query: 121 SPKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGA 180
           +PKKYDEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGA
Sbjct: 121 APKKYDEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA 180

Query: 181 ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYE 240
           ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNK+DQVDDEELLELVELE+RELLSSYE
Sbjct: 181 ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLELVELEVRELLSSYE 240

Query: 241 FPGDDVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFL 300
           FPGD++PII+GSALLALEALMAN +I RGEN+WVDKI++LMD VD YIPIP+RQT+LPFL
Sbjct: 241 FPGDEIPIISGSALLALEALMANPSIKRGENQWVDKIYQLMDNVDEYIPIPQRQTELPFL 300

Query: 301 LAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAG 360
           +A+EDVFSITGRGTVATGRVERGTV+VGE VDIVGL++TRNTTVTGVEMFQKILDEA+AG
Sbjct: 301 MAIEDVFSITGRGTVATGRVERGTVKVGEIVDIVGLKDTRNTTVTGVEMFQKILDEAMAG 360

Query: 361 DNVGCCFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFY 420
           DNVG                  +     PHTKF A+VYVLKKEEGGRHSPFFAGYRPQFY
Sbjct: 361 DNVGLLLRGIQKIDIQRGMVLAKPGTITPHTKFEALVYVLKKEEGGRHSPFFAGYRPQFY 420

Query: 421 MRTTDVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAG 449
           MRTTDVTGKV+ IM+DK EESKMVMPGDRV MVVELIMPVACEQGMRFAIREGGKTVGAG
Sbjct: 421 MRTTDVTGKVTVIMSDKGEESKMVMPGDRVNMVVELIMPVACEQGMRFAIREGGKTVGAG 480

BLAST of PI0009499 vs. ExPASy Swiss-Prot
Match: O24310 (Elongation factor Tu, chloroplastic OS=Pisum sativum OX=3888 GN=tufA PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 8.1e-188
Identity = 358/488 (73.36%), Postives = 402/488 (82.38%), Query Frame = 0

Query: 3   AISLASVSTSSKLVFPHPSSSSST---TSSSSLSSSLLSKPS---SNLSSSFLNPSSIRP 62
           A+S  + +TSSKL   +P S S T   ++S+S+S+S   +P    + LSSSFLNPS+I  
Sbjct: 2   ALSSTAATTSSKLKLSNPPSLSHTFTASASASVSNSTSFRPKLTLTRLSSSFLNPSTILH 61

Query: 63  LSFSSPSVNRPRS--FTIRAARGKFERKKPHVNIGTIGH--------------------- 122
           L+  S   NRP S  FT+RAARGKFERKKPH+NIGTIGH                     
Sbjct: 62  LT-PSQRTNRPSSSPFTVRAARGKFERKKPHLNIGTIGHVDHGKTTLTAALTMALACLGN 121

Query: 123 QSPKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDG 182
            +PKKYDEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDG
Sbjct: 122 SAPKKYDEIDAAPEERARGITINTATVEYETETRHYAHVDCPGHADYVKNMITGAAQMDG 181

Query: 183 AILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSY 242
           AILVVSGADGPMPQTKEHILLAKQVGVP++VVFLNK+DQVDDEELLELVELE+RELLSSY
Sbjct: 182 AILVVSGADGPMPQTKEHILLAKQVGVPSVVVFLNKQDQVDDEELLELVELEVRELLSSY 241

Query: 243 EFPGDDVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPF 302
           EFPGDD+PI++GSALLALEALMAN  + RG N+WVDKI++LMD VD YIPIP+RQT+LPF
Sbjct: 242 EFPGDDIPIVSGSALLALEALMANPTLKRGNNQWVDKIYQLMDEVDKYIPIPQRQTELPF 301

Query: 303 LLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALA 362
           LLA+EDVFSIT RGTVATGR+ERG V+VG+ VD+VGLRETRNTTVTGVEMFQKILD+A+A
Sbjct: 302 LLAIEDVFSITXRGTVATGRIERGLVKVGDVVDLVGLRETRNTTVTGVEMFQKILDDAMA 361

Query: 363 GDNVGCCFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQF 422
           GDNVG                  +     PH+KFSA+VYVLKKEEGGRHSPFFAGYRPQF
Sbjct: 362 GDNVGLLLRGIQKIDIQRGMVLAKPGTITPHSKFSAIVYVLKKEEGGRHSPFFAGYRPQF 421

Query: 423 YMRTTDVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGA 449
           YMRTTDVTGKV+SIMNDKDEESKMVMPGDRVK+VVELI+PVA EQGMRFAIREGGKTVGA
Sbjct: 422 YMRTTDVTGKVTSIMNDKDEESKMVMPGDRVKIVVELIVPVAIEQGMRFAIREGGKTVGA 481

BLAST of PI0009499 vs. ExPASy Swiss-Prot
Match: Q40450 (Elongation factor TuA, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFA PE=2 SV=2)

HSP 1 Score: 657.1 bits (1694), Expect = 1.4e-187
Identity = 354/482 (73.44%), Postives = 395/482 (81.95%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFS 60
           MA+IS A+ ++S+KLV  + ++    +S+      L S  + N S+ FL+     P + S
Sbjct: 1   MASISAATATSSTKLVSSNSTNPLLPSSTKPSKLILSSSFTPNCSTLFLH----SPATPS 60

Query: 61  SPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH---------------------QSPKKY 120
           S + +R R FT+RAARGKFERKKPHVNIGTIGH                      +PKKY
Sbjct: 61  STATHRHRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASMGNSAPKKY 120

Query: 121 DEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVS 180
           DEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILV S
Sbjct: 121 DEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVCS 180

Query: 181 GADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDD 240
           GADGPMPQTKEHILLAKQVGVPNMVVFLNK+DQVDDEELL+LVELE+RELLSSYEFPGDD
Sbjct: 181 GADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDD 240

Query: 241 VPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVED 300
           +PII+GSALLALEALMAN +I RGEN+WVDKI+ELMDAVDSYIPIP RQT+LPFL+A+ED
Sbjct: 241 IPIISGSALLALEALMANPSIKRGENQWVDKIYELMDAVDSYIPIPVRQTELPFLMAIED 300

Query: 301 VFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGC 360
           VFSITGRGTVATGRVERGTVR+G+TVDIVGL++TR+TTVTGVEMFQKILDEA+AGDNVG 
Sbjct: 301 VFSITGRGTVATGRVERGTVRIGDTVDIVGLKDTRSTTVTGVEMFQKILDEAMAGDNVGL 360

Query: 361 CFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTD 420
                            +     PHTKF A+VYVLKKEEGGRHSPFF+GYRPQFYMRTTD
Sbjct: 361 LLRGIQKIDIQRGMVLAKPGTITPHTKFEAIVYVLKKEEGGRHSPFFSGYRPQFYMRTTD 420

Query: 421 VTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSI 449
           VTGKV+SI  DK EESKMVMPGDRV +VVELIMPVACEQGMRFAIREGGKTVGAGVIQ I
Sbjct: 421 VTGKVTSITTDKGEESKMVMPGDRVNLVVELIMPVACEQGMRFAIREGGKTVGAGVIQKI 478

BLAST of PI0009499 vs. ExPASy TrEMBL
Match: A0A5A7URR7 (Elongation factor Tu OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001070 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 427/480 (88.96%), Postives = 431/480 (89.79%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSS--SSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLS 60
           MAAISLASVSTSSKLVFPHPSS  S S +SSSS SSSL SKPSSNLSSSFLN SSIRP S
Sbjct: 1   MAAISLASVSTSSKLVFPHPSSSPSPSPSSSSSPSSSLFSKPSSNLSSSFLNSSSIRPFS 60

Query: 61  FSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYDE 120
           FSSPSVNRPRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKYDE
Sbjct: 61  FSSPSVNRPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240

Query: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300
           IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 360
           SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
                          +     PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLAKPGTITPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 480

BLAST of PI0009499 vs. ExPASy TrEMBL
Match: A0A1S3CLM5 (Elongation factor Tu OS=Cucumis melo OX=3656 GN=LOC103502373 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 427/480 (88.96%), Postives = 431/480 (89.79%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSS--SSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLS 60
           MAAISLASVSTSSKLVFPHPSS  S S +SSSS SSSL SKPSSNLSSSFLN SSIRP S
Sbjct: 1   MAAISLASVSTSSKLVFPHPSSSPSPSPSSSSSPSSSLFSKPSSNLSSSFLNSSSIRPFS 60

Query: 61  FSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYDE 120
           FSSPSVNRPRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKYDE
Sbjct: 61  FSSPSVNRPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240

Query: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300
           IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 360
           SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
                          +     PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLAKPGTITPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 480

BLAST of PI0009499 vs. ExPASy TrEMBL
Match: A0A6J1E4U0 (Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111429936 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.4e-212
Identity = 410/484 (84.71%), Postives = 419/484 (86.57%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSN------LSSSFLNPSSI 60
           MAAISLAS STSS L+F H S+S     SSS SSSL  KPSS+      LSSSFLNPSSI
Sbjct: 1   MAAISLASASTSSNLLFLHASAS----PSSSPSSSLFVKPSSSSSSAIKLSSSFLNPSSI 60

Query: 61  RPLSFSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPK 120
           RPL+FSSP+V  PRS TIRAARGKFERKKPHVNIGTIGH                 ++PK
Sbjct: 61  RPLTFSSPAVKPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPK 120

Query: 121 KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV 180
           KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV
Sbjct: 121 KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV 180

Query: 181 VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG 240
           VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG
Sbjct: 181 VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG 240

Query: 241 DDVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV 300
           DDVPIIAGSALLALEALMAN  I RGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV
Sbjct: 241 DDVPIIAGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV 300

Query: 301 EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV 360
           EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV
Sbjct: 301 EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV 360

Query: 361 GCCFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRT 420
           G                  +     PHTKF AVVYVLKKEEGGRHSPFFAGYRPQFYMRT
Sbjct: 361 GLLLRGVQKADIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRT 420

Query: 421 TDVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ 449
           TDVTGKV+SIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ
Sbjct: 421 TDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ 480

BLAST of PI0009499 vs. ExPASy TrEMBL
Match: A0A6J1C0N2 (Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111007263 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 411/483 (85.09%), Postives = 422/483 (87.37%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSS--NLSSSFLNPSSIRPLS 60
           MAAISLAS STS+KLVFPH S      S SS  +SL +KPSS   LSSSFLNPSSIRPL+
Sbjct: 1   MAAISLASASTSTKLVFPHAS-----PSPSSPPASLFAKPSSIPKLSSSFLNPSSIRPLT 60

Query: 61  FSSPS---VNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKK 120
           FSS S   V RPRSFTIRAARGKFERKKPHVNIGTIGH                 ++PKK
Sbjct: 61  FSSGSSATVARPRSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKK 120

Query: 121 YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV 180
           YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV
Sbjct: 121 YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV 180

Query: 181 SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGD 240
           SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELL+LVELEMRELLSSYEFPGD
Sbjct: 181 SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLQLVELEMRELLSSYEFPGD 240

Query: 241 DVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVE 300
           DVPIIAGSALLALEALMAN +IARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVE
Sbjct: 241 DVPIIAGSALLALEALMANQSIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVE 300

Query: 301 DVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG 360
           DVFSITGRGTVATGRVERGT+RVG+TVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG
Sbjct: 301 DVFSITGRGTVATGRVERGTIRVGDTVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG 360

Query: 361 CCFE-VQAWHYH------------PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTT 420
                VQ                 PHTKF AVVYVLKKEEGGRHSPFFAGYRPQFYMRTT
Sbjct: 361 LLLRGVQKADIQRGMVLSKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTT 420

Query: 421 DVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS 449
           DVTGKV+SIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS
Sbjct: 421 DVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS 478

BLAST of PI0009499 vs. ExPASy TrEMBL
Match: A0A6J1JDL1 (Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111484851 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 405/478 (84.73%), Postives = 417/478 (87.24%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFS 60
           MAAISLAS STSS L+F H S+S S++ SSSL +   S  +  LSSSFLNPSSIRPL+FS
Sbjct: 1   MAAISLASASTSSNLLFLHASASPSSSPSSSLFAKPSSSSAIKLSSSFLNPSSIRPLTFS 60

Query: 61  SPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYDEID 120
           S +VN PRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKYDEID
Sbjct: 61  SSAVNPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEID 120

Query: 121 AAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADG 180
           AAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADG
Sbjct: 121 AAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADG 180

Query: 181 PMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPII 240
           PMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPII
Sbjct: 181 PMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPII 240

Query: 241 AGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSI 300
           AGSALLALEALMAN  I RGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSI
Sbjct: 241 AGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSI 300

Query: 301 TGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCFE- 360
           TGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG     
Sbjct: 301 TGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRG 360

Query: 361 ------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGK 420
                        +     PHTKF AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGK
Sbjct: 361 VQKADIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGK 420

Query: 421 VSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           V+SIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 VTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 478

BLAST of PI0009499 vs. NCBI nr
Match: XP_004138088.2 (elongation factor Tu, chloroplastic [Cucumis sativus] >KAE8652395.1 hypothetical protein Csa_014228 [Cucumis sativus])

HSP 1 Score: 786.6 bits (2030), Expect = 1.2e-223
Identity = 427/482 (88.59%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSS----SSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRP 60
           MAAISLASVSTSSKLVFPHPSSS    SS++SSSSLSS+L SKPSSNLSSSFLN SSIRP
Sbjct: 1   MAAISLASVSTSSKLVFPHPSSSSSSPSSSSSSSSLSSTLFSKPSSNLSSSFLNSSSIRP 60

Query: 61  LSFSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKY 120
           LSFSSPSV+RPRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKY
Sbjct: 61  LSFSSPSVSRPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKY 120

Query: 121 DEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVS 180
           DEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVS
Sbjct: 121 DEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVS 180

Query: 181 GADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDD 240
           GADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDD
Sbjct: 181 GADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDD 240

Query: 241 VPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVED 300
           VPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVED
Sbjct: 241 VPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVED 300

Query: 301 VFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGC 360
           VFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG 
Sbjct: 301 VFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGL 360

Query: 361 CFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTD 420
                            +     PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTD
Sbjct: 361 LLRGVQKADIQRGMVLAKPGTITPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTD 420

Query: 421 VTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSI 449
           VTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSI
Sbjct: 421 VTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSI 480

BLAST of PI0009499 vs. NCBI nr
Match: XP_008464512.1 (PREDICTED: elongation factor Tu, chloroplastic [Cucumis melo] >KAA0057820.1 elongation factor Tu [Cucumis melo var. makuwa] >TYJ98504.1 elongation factor Tu [Cucumis melo var. makuwa])

HSP 1 Score: 784.6 bits (2025), Expect = 4.4e-223
Identity = 427/480 (88.96%), Postives = 431/480 (89.79%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSS--SSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLS 60
           MAAISLASVSTSSKLVFPHPSS  S S +SSSS SSSL SKPSSNLSSSFLN SSIRP S
Sbjct: 1   MAAISLASVSTSSKLVFPHPSSSPSPSPSSSSSPSSSLFSKPSSNLSSSFLNSSSIRPFS 60

Query: 61  FSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYDE 120
           FSSPSVNRPRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKYDE
Sbjct: 61  FSSPSVNRPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240

Query: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300
           IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 360
           SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
                          +     PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLAKPGTITPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 480

BLAST of PI0009499 vs. NCBI nr
Match: XP_038879216.1 (elongation factor Tu, chloroplastic [Benincasa hispida])

HSP 1 Score: 772.3 bits (1993), Expect = 2.3e-219
Identity = 420/480 (87.50%), Postives = 429/480 (89.38%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSN--LSSSFLNPSSIRPLS 60
           MAAISLAS STSSKL+FPHPSSSSS++SSSS      S PSS+  LSSSFLNPSSIRPLS
Sbjct: 1   MAAISLASASTSSKLLFPHPSSSSSSSSSSS-----SSPPSSSLKLSSSFLNPSSIRPLS 60

Query: 61  FSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYDE 120
           FSSPS+NRPRSFTIRAARGKFERKKPHVNIGTIGH                 + PKKYDE
Sbjct: 61  FSSPSLNRPRSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKVPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELL+LVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLQLVELEMRELLSSYEFPGDDVP 240

Query: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300
           IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCCF 360
           SITGRGTVATGRVERGTV+VGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG   
Sbjct: 301 SITGRGTVATGRVERGTVKVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 E-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
                          +     PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLAKPGTITPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 449
           GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475

BLAST of PI0009499 vs. NCBI nr
Match: KAG6589415.1 (Elongation factor Tu, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 749.6 bits (1934), Expect = 1.6e-212
Identity = 411/481 (85.45%), Postives = 420/481 (87.32%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSN---LSSSFLNPSSIRPL 60
           MAAISLAS STSS L+F H S+S     SSS SSSL  KPSS+   LSSSFLNPSSIRPL
Sbjct: 1   MAAISLASASTSSNLLFLHASAS----PSSSPSSSLFVKPSSSAIKLSSSFLNPSSIRPL 60

Query: 61  SFSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPKKYD 120
           +FSSP+VN PRS TIRAARGKFERKKPHVNIGTIGH                 ++PKKYD
Sbjct: 61  TFSSPAVNPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYD 120

Query: 121 EIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSG 180
           EIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSG
Sbjct: 121 EIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSG 180

Query: 181 ADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDV 240
           ADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDV
Sbjct: 181 ADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDV 240

Query: 241 PIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDV 300
           PIIAGSALLALEALMAN  I RGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDV
Sbjct: 241 PIIAGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDV 300

Query: 301 FSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGCC 360
           FSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG  
Sbjct: 301 FSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLL 360

Query: 361 FE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDV 420
                           +     PHTKF AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDV
Sbjct: 361 LRGVQKADIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDV 420

Query: 421 TGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSII 449
           TGKV+SIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSII
Sbjct: 421 TGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSII 477

BLAST of PI0009499 vs. NCBI nr
Match: XP_023516393.1 (elongation factor Tu, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 748.0 bits (1930), Expect = 4.6e-212
Identity = 413/484 (85.33%), Postives = 421/484 (86.98%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSN------LSSSFLNPSSI 60
           MAAISLAS STSS L+F H S+S    SSS  SSSL  KPSS+      LSSSFLNPSSI
Sbjct: 1   MAAISLASASTSSNLLFLHASAS---PSSSPSSSSLFVKPSSSSSSAIKLSSSFLNPSSI 60

Query: 61  RPLSFSSPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH-----------------QSPK 120
           RPL+FSSP+VN PRS TIRAARGKFERKKPHVNIGTIGH                 ++PK
Sbjct: 61  RPLTFSSPAVNPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPK 120

Query: 121 KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV 180
           KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV
Sbjct: 121 KYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILV 180

Query: 181 VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG 240
           VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG
Sbjct: 181 VSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPG 240

Query: 241 DDVPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV 300
           DDVPIIAGSALLALEALMAN  I RGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV
Sbjct: 241 DDVPIIAGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAV 300

Query: 301 EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV 360
           EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV
Sbjct: 301 EDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNV 360

Query: 361 GCCFE-VQAWHYH------------PHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRT 420
           G     VQ                 PHTKF AVVYVLKKEEGGRHSPFFAGYRPQFYMRT
Sbjct: 361 GLLLRGVQKADIQRGMVLSKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRT 420

Query: 421 TDVTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ 449
           TDVTGKV+SIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ
Sbjct: 421 TDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQ 480

BLAST of PI0009499 vs. TAIR 10
Match: AT4G20360.1 (RAB GTPase homolog E1B )

HSP 1 Score: 641.7 bits (1654), Expect = 4.3e-184
Identity = 352/482 (73.03%), Postives = 389/482 (80.71%), Query Frame = 0

Query: 1   MAAISLASVSTSSKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFS 60
           MA  + A+ S+SS+++  + S S S   + S S  L    +  LSSSFL   S   L+ +
Sbjct: 1   MAISAPAACSSSSRILCSYSSPSPSLCPAISTSGKL---KTLTLSSSFLPSYS---LTTT 60

Query: 61  SPSVNRPRSFTIRAARGKFERKKPHVNIGTIGH---------------------QSPKKY 120
           S S +  RSFT+RAARGKFERKKPHVNIGTIGH                        KKY
Sbjct: 61  SASQSTRRSFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKY 120

Query: 121 DEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVS 180
           DEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVS
Sbjct: 121 DEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVS 180

Query: 181 GADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDD 240
           GADGPMPQTKEHILLAKQVGVP+MVVFLNK+DQVDD ELLELVELE+RELLSSYEF GDD
Sbjct: 181 GADGPMPQTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSYEFNGDD 240

Query: 241 VPIIAGSALLALEALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVED 300
           +PII+GSALLA+E L  N  + RG+N+WVDKI+ELMDAVD YIPIP+RQT+LPFLLAVED
Sbjct: 241 IPIISGSALLAVETLTENPKVKRGDNKWVDKIYELMDAVDDYIPIPQRQTELPFLLAVED 300

Query: 301 VFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGC 360
           VFSITGRGTVATGRVERGTV+VGETVD+VGLRETR+ TVTGVEMFQKILDEALAGDNVG 
Sbjct: 301 VFSITGRGTVATGRVERGTVKVGETVDLVGLRETRSYTVTGVEMFQKILDEALAGDNVGL 360

Query: 361 CFE-------------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTD 420
                            +     PHTKF A++YVLKKEEGGRHSPFFAGYRPQFYMRTTD
Sbjct: 361 LLRGIQKADIQRGMVLAKPGSITPHTKFEAIIYVLKKEEGGRHSPFFAGYRPQFYMRTTD 420

Query: 421 VTGKVSSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSI 449
           VTGKV+ IMNDKDEESKMVMPGDRVK+VVELI+PVACEQGMRFAIREGGKTVGAGVI +I
Sbjct: 421 VTGKVTKIMNDKDEESKMVMPGDRVKIVVELIVPVACEQGMRFAIREGGKTVGAGVIGTI 476

BLAST of PI0009499 vs. TAIR 10
Match: AT4G02930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 438.0 bits (1125), Expect = 9.4e-123
Identity = 257/472 (54.45%), Postives = 306/472 (64.83%), Query Frame = 0

Query: 13  SKLVFPHPSSSSSTTSSSSLSSSLLSKPSSNLSSSFLNPSSIRPLSFSSPSVNRPRSFTI 72
           + +V  +PSS          SS + S+  ++++SS+    SI     SS +      +  
Sbjct: 2   ASVVLRNPSSKRLV----PFSSQIYSRCGASVTSSYSISHSIGGDDLSSSTFGTSSFWRS 61

Query: 73  RAARGKFERKKPHVNIGTIGHQSPKK---------------------YDEIDAAPEERAR 132
            A    F R KPHVN+GTIGH    K                     +DEID APEE+ R
Sbjct: 62  MAT---FTRNKPHVNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKR 121

Query: 133 GITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEH 192
           GITI TA VEYET  RHYAHVDCPGHADYVKNMITGAAQMDG ILVVSG DGPMPQTKEH
Sbjct: 122 GITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMPQTKEH 181

Query: 193 ILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIIAGSALLAL 252
           ILLA+QVGVP++V FLNK D VDD ELLELVE+E+RELLS Y+FPGDD+PII GSAL AL
Sbjct: 182 ILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKFPGDDIPIIRGSALSAL 241

Query: 253 EALMANNNIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSITGRGTVAT 312
           +    N+ I R        I +LMDAVD YIP P R  D PFL+ +EDVFSI GRGTVAT
Sbjct: 242 QG--TNDEIGR------QAILKLMDAVDEYIPDPVRVLDKPFLMPIEDVFSIQGRGTVAT 301

Query: 313 GRVERGTVRVGETVDIVGLRE---TRNTTVTGVEMFQKILDEALAGDNVGCCFE------ 372
           GR+E+G ++VGE V+I+GLRE      +TVTGVEMF+KILD   AGDNVG          
Sbjct: 302 GRIEQGVIKVGEEVEILGLREGGVPLKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKRED 361

Query: 373 -------VQAWHYHPHTKFSAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVSSIM 432
                   +      + KF A +YVL K+EGGRH+ FF+ YRPQFY+RT D+TGKV    
Sbjct: 362 IQRGMVIAKPGSCKTYKKFEAEIYVLTKDEGGRHTAFFSNYRPQFYLRTADITGKV---- 421

Query: 433 NDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSII 448
            +  E  KMVMPGD V  V ELIMPV  E G RFA+REGG+TVGAGV+  ++
Sbjct: 422 -ELPENVKMVMPGDNVTAVFELIMPVPLETGQRFALREGGRTVGAGVVSKVM 453

BLAST of PI0009499 vs. TAIR 10
Match: AT1G07920.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 132.9 bits (333), Expect = 6.5e-31
Identity = 115/385 (29.87%), Postives = 174/385 (45.19%), Query Frame = 0

Query: 102 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 161
           +D    ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++   
Sbjct: 60  LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119

Query: 162 DGPMP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLS 221
            G          QT+EH LLA  +GV  M+   NK D       +   + +  E+   L 
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLK 179

Query: 222 SYEFPGDDVPIIAGSALLALEALMANNNIARGEN-EWVDKIFELMDAVDSYIPIPERQTD 281
              +  D +P +       +     +N I R  N +W  K   L++A+D  I  P+R +D
Sbjct: 180 KVGYNPDKIPFV------PISGFEGDNMIERSTNLDWY-KGPTLLEALDQ-INEPKRPSD 239

Query: 282 LPFLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDE 341
            P  L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L E
Sbjct: 240 KPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLE 299

Query: 342 ALAGDNVG------CCFEVQAWHYHPHTK---------FSAVVYVLKKEEGGRHSPFFAG 401
           AL GDNVG         +++  +   ++K         F++ V ++             G
Sbjct: 300 ALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNG 359

Query: 402 YRPQFYMRTTDVTGKVSSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM 447
           Y P     T+ +  K S I+   D        +E K +  GD   + +    P+  E   
Sbjct: 360 YAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFS 419

BLAST of PI0009499 vs. TAIR 10
Match: AT1G07930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 132.9 bits (333), Expect = 6.5e-31
Identity = 115/385 (29.87%), Postives = 174/385 (45.19%), Query Frame = 0

Query: 102 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 161
           +D    ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++   
Sbjct: 60  LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119

Query: 162 DGPMP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLS 221
            G          QT+EH LLA  +GV  M+   NK D       +   + +  E+   L 
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLK 179

Query: 222 SYEFPGDDVPIIAGSALLALEALMANNNIARGEN-EWVDKIFELMDAVDSYIPIPERQTD 281
              +  D +P +       +     +N I R  N +W  K   L++A+D  I  P+R +D
Sbjct: 180 KVGYNPDKIPFV------PISGFEGDNMIERSTNLDWY-KGPTLLEALDQ-INEPKRPSD 239

Query: 282 LPFLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDE 341
            P  L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L E
Sbjct: 240 KPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLE 299

Query: 342 ALAGDNVG------CCFEVQAWHYHPHTK---------FSAVVYVLKKEEGGRHSPFFAG 401
           AL GDNVG         +++  +   ++K         F++ V ++             G
Sbjct: 300 ALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNG 359

Query: 402 YRPQFYMRTTDVTGKVSSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM 447
           Y P     T+ +  K S I+   D        +E K +  GD   + +    P+  E   
Sbjct: 360 YAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFS 419

BLAST of PI0009499 vs. TAIR 10
Match: AT1G07940.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 132.9 bits (333), Expect = 6.5e-31
Identity = 115/385 (29.87%), Postives = 174/385 (45.19%), Query Frame = 0

Query: 102 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 161
           +D    ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++   
Sbjct: 60  LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119

Query: 162 DGPMP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLS 221
            G          QT+EH LLA  +GV  M+   NK D       +   + +  E+   L 
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLK 179

Query: 222 SYEFPGDDVPIIAGSALLALEALMANNNIARGEN-EWVDKIFELMDAVDSYIPIPERQTD 281
              +  D +P +       +     +N I R  N +W  K   L++A+D  I  P+R +D
Sbjct: 180 KVGYNPDKIPFV------PISGFEGDNMIERSTNLDWY-KGPTLLEALDQ-INEPKRPSD 239

Query: 282 LPFLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDE 341
            P  L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L E
Sbjct: 240 KPLRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLE 299

Query: 342 ALAGDNVG------CCFEVQAWHYHPHTK---------FSAVVYVLKKEEGGRHSPFFAG 401
           AL GDNVG         +++  +   ++K         F++ V ++             G
Sbjct: 300 ALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNG 359

Query: 402 YRPQFYMRTTDVTGKVSSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM 447
           Y P     T+ +  K S I+   D        +E K +  GD   + +    P+  E   
Sbjct: 360 YAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFS 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q434673.4e-19476.04Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFA PE=3 SV=1[more]
P462801.6e-19175.00Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFB1 PE=3 SV=1[more]
Q433641.9e-18974.74Elongation factor TuB, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFB PE=... [more]
O243108.1e-18873.36Elongation factor Tu, chloroplastic OS=Pisum sativum OX=3888 GN=tufA PE=2 SV=1[more]
Q404501.4e-18773.44Elongation factor TuA, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFA PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7URR72.1e-22388.96Elongation factor Tu OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350... [more]
A0A1S3CLM52.1e-22388.96Elongation factor Tu OS=Cucumis melo OX=3656 GN=LOC103502373 PE=3 SV=1[more]
A0A6J1E4U08.4e-21284.71Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111429936 PE=3 SV=1[more]
A0A6J1C0N21.1e-21185.09Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111007263 PE=3 SV=1[more]
A0A6J1JDL11.4e-21184.73Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111484851 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004138088.21.2e-22388.59elongation factor Tu, chloroplastic [Cucumis sativus] >KAE8652395.1 hypothetical... [more]
XP_008464512.14.4e-22388.96PREDICTED: elongation factor Tu, chloroplastic [Cucumis melo] >KAA0057820.1 elon... [more]
XP_038879216.12.3e-21987.50elongation factor Tu, chloroplastic [Benincasa hispida][more]
KAG6589415.11.6e-21285.45Elongation factor Tu, chloroplastic, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023516393.14.6e-21285.33elongation factor Tu, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G20360.14.3e-18473.03RAB GTPase homolog E1B [more]
AT4G02930.19.4e-12354.45GTP binding Elongation factor Tu family protein [more]
AT1G07920.16.5e-3129.87GTP binding Elongation factor Tu family protein [more]
AT1G07930.16.5e-3129.87GTP binding Elongation factor Tu family protein [more]
AT1G07940.16.5e-3129.87GTP binding Elongation factor Tu family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004541Translation elongation factor EFTu/EF1A, bacterial/organelleTIGRFAMTIGR00485TIGR00485coord: 96..448
e-value: 4.1E-181
score: 599.9
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 350..448
e-value: 1.9E-40
score: 139.1
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 269..348
e-value: 1.7E-23
score: 84.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availablePANTHERPTHR43721ELONGATION FACTOR TU-RELATEDcoord: 31..448
NoneNo IPR availablePANTHERPTHR43721:SF5ELONGATION FACTOR TU, CHLOROPLASTICcoord: 31..448
NoneNo IPR availableCDDcd03707EFTU_IIIcoord: 349..443
e-value: 7.66166E-55
score: 175.393
IPR004161Translation elongation factor EFTu-like, domain 2PFAMPF03144GTP_EFTU_D2coord: 287..339
e-value: 8.7E-13
score: 48.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 75..265
e-value: 4.4E-58
score: 198.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 78..281
IPR000795Translational (tr)-type GTP-binding domainPFAMPF00009GTP_EFTUcoord: 95..263
e-value: 1.7E-42
score: 145.2
IPR000795Translational (tr)-type GTP-binding domainPROSITEPS51722G_TR_2coord: 103..266
score: 42.081429
IPR004160Translation elongation factor EFTu/EF1A, C-terminalPFAMPF03143GTP_EFTU_D3coord: 349..446
e-value: 3.7E-39
score: 133.3
IPR031157Tr-type G domain, conserved sitePROSITEPS00301G_TR_1coord: 103..118
IPR033720Elongation factor Tu, domain 2CDDcd03697EFTU_IIcoord: 273..339
e-value: 3.44513E-37
score: 129.176
IPR009001Translation elongation factor EF1A/initiation factor IF2gamma, C-terminalSUPERFAMILY50465EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domaincoord: 348..447
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 267..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009499.1PI0009499.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070125 mitochondrial translational elongation
biological_process GO:0006414 translational elongation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003746 translation elongation factor activity