PI0009067 (gene) Melon (PI 482460) v1

Overview
NamePI0009067
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGlucan endo-1,3-beta-glucosidase
Locationchr09: 137218 .. 139054 (-)
RNA-Seq ExpressionPI0009067
SyntenyPI0009067
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAATGCCACACCCAAATTCACTCTCTTCTTCCAAATCCATCTTCATCACCTCCATCCGATGGCTTCCCCTCCTTTCACTCCCTCACATCACCTTCTTCTACCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCTCTACACTCATCCAAATTCAAGGTAAATTAATTACATCATACATTATTCTTATGTGCTTTTCTTTTCACTACCATTTTTTTAAAAATAAAAAATTAACTTTCTTCAGGAGTTTATGGTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCAACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCACTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACTAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATCGAAGGATTCAAATCTCAACGCCCCACTCTCTCGGCATTTTGTCGAACTCAACCCCGCCGTCAACTGGAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTCAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGAAAATCTGGATTACGCACTCTTCCGACGGAATCCCGGCATGTTCGACTCGGATTTAGGGATTTTATACGCCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGTTTGGGCTTTGAGGATCTTGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGACCCGACCCAAGTTGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTCATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAAGATCTCAAACCCGGTCCAGTTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGACCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGTAGCTGCCACAGCTCGAACCCACCCTAAAACGTTTTTGATAATCATGATCGTTACATGTTGGTTGATTGGTTTGTTGTCGACTTGGATCTAACAGGCTCAATCCGCTACTCCGAGAGCCCATCACAGTCCAGTTGAAGGCCCAATATCTACACCAAGAAGCTCAAGCCCAAGTCCAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAACTGAGGCCAACGTGGAGGCCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACGGGCGCTCTAACCACGGTGGATCCAAGTAAGTCCCATTGTTTTTGTTTTTGCTTTTGTTCTTTTTTTTTTTTAAATGCTTAAATTATTATTAATTAAAATTCATTTACTAATTCTGTAATTTAATTATTCATAAAAGTATTTTCTTTAGCAATTTGTTTTGGTTTTAATTTTTCAGGTTATGGGAAGTGCAAATATTGGTAA

mRNA sequence

ATGGCCAATGCCACACCCAAATTCACTCTCTTCTTCCAAATCCATCTTCATCACCTCCATCCGATGGCTTCCCCTCCTTTCACTCCCTCACATCACCTTCTTCTACCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCTCTACACTCATCCAAATTCAAGGAGTTTATGGTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCAACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCACTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACTAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATCGAAGGATTCAAATCTCAACGCCCCACTCTCTCGGCATTTTGTCGAACTCAACCCCGCCGTCAACTGGAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTCAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGAAAATCTGGATTACGCACTCTTCCGACGGAATCCCGGCATGTTCGACTCGGATTTAGGGATTTTATACGCCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGTTTGGGCTTTGAGGATCTTGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGACCCGACCCAAGTTGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTCATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAAGATCTCAAACCCGGTCCAGTTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGACCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGCTCAATCCGCTACTCCGAGAGCCCATCACAGTCCAGTTGAAGGCCCAATATCTACACCAAGAAGCTCAAGCCCAAGTCCAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAACTGAGGCCAACGTGGAGGCCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACGGGCGCTCTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Coding sequence (CDS)

ATGGCCAATGCCACACCCAAATTCACTCTCTTCTTCCAAATCCATCTTCATCACCTCCATCCGATGGCTTCCCCTCCTTTCACTCCCTCACATCACCTTCTTCTACCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCTCTACACTCATCCAAATTCAAGGAGTTTATGGTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCAACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCACTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACTAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCTCTCATTGAAGCCTCCTTGGATCGAAGGATTCAAATCTCAACGCCCCACTCTCTCGGCATTTTGTCGAACTCAACCCCGCCGTCAACTGGAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTCAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGAAAATCTGGATTACGCACTCTTCCGACGGAATCCCGGCATGTTCGACTCGGATTTAGGGATTTTATACGCCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGTTTGGGCTTTGAGGATCTTGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGACCCGACCCAAGTTGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTCATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAAGATCTCAAACCCGGTCCAGTTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGACCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGCTCAATCCGCTACTCCGAGAGCCCATCACAGTCCAGTTGAAGGCCCAATATCTACACCAAGAAGCTCAAGCCCAAGTCCAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAACTGAGGCCAACGTGGAGGCCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACGGGCGCTCTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Protein sequence

MANATPKFTLFFQIHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKYW
Homology
BLAST of PI0009067 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.4e-126
Identity = 224/443 (50.56%), Postives = 297/443 (67.04%), Query Frame = 0

Query: 63  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQI 122
           IGVNYG  ADNLP P+ VA FL   T IDRV+LFDA+P  + AFA T IS+++++PN  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 123 PHLVKP----NFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHT 182
           P L       + A  WI+ N+ PY+PATN+  +L GNE+L + +  LI +L+PAM+ L  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 183 ALIEASLDRRIQISTPHSLGILSNSTP-PSTGRFRQGYDTHVIKPMLSFLRETNSPLMVN 242
           AL    L   ++++TPH LGIL+ S   PS   FR GY+T +   ML F R+T SP MVN
Sbjct: 146 ALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 243 PYPFFACTAENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIA 302
           PYP+F+   E L+YALFR N G++D    + Y +MLDAQ+DA+Y+AMK LG+ D+DI + 
Sbjct: 206 PYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVDIAVG 265

Query: 303 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPG 362
           E GWP++ +P Q+GVG +EA  +N  ++R   SGKGTPLMPNRTFETY+F+LF+E+ KPG
Sbjct: 266 EAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPG 325

Query: 363 PVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRW 422
           P+ ER+FGLF PD TPVY++G+LR  A  A       P   P  +P  S     S   +W
Sbjct: 326 PIAERHFGLFNPDFTPVYDLGLLRDGASVA-------PTPSPNPSPNPSPKPAPSGGGKW 385

Query: 423 CLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGND 482
           C+ K  AN   LQ NI+Y CG  +DC PIQ  G CF+PN+++AHA+YVMNAY+QA    D
Sbjct: 386 CVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTD 445

Query: 483 FDCDFDQTGALTTVDPSYGKCKY 501
             CDF  TG +T+ DPSYG CKY
Sbjct: 446 LACDFKGTGIVTSSDPSYGGCKY 459

BLAST of PI0009067 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 6.0e-115
Identity = 210/443 (47.40%), Postives = 283/443 (63.88%), Query Frame = 0

Query: 63  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQI 122
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 123 PHLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 182
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 183 EASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 242
             SL  +I++ST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 243 FACTA----ENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIA 302
           FA  +    E L + LF  N G  DS  GI Y NM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 303 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPG 362
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 363 PVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRW 422
           P  ER FGLF+ DL+ VY++G+ + ++ S TP        G ++            S  W
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP-------SGKVT------------SSGW 385

Query: 423 CLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGND 482
           C+PK  A  E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +    
Sbjct: 386 CVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQP 445

Query: 483 FDCDFDQTGALTTVDPSYGKCKY 501
            DCDF +T  +T+ +PSY  C Y
Sbjct: 446 TDCDFSKTATVTSQNPSYNNCVY 447

BLAST of PI0009067 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-111
Identity = 201/463 (43.41%), Postives = 292/463 (63.07%), Query Frame = 0

Query: 47  LILSTLIQIQGVYGGS----IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNI 106
           L+    + +Q  Y  +    +GVNYG ++DNL P  +    LL +T I +VRLF A+P +
Sbjct: 10  LLFLVFLLLQNFYSANSQSFLGVNYGQLSDNL-PSLQATVNLLKSTTIQKVRLFGAEPAV 69

Query: 107 LRAFAHTGISVSITIPNDQIPHLV-KPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTA 166
           ++AFA+TG+ + I   N  IP L   PN A +++K N+  + PA+NII + VGNEVL++ 
Sbjct: 70  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSG 129

Query: 167 NKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIK 226
           ++ LI+ L+PAMQ++  AL  ASL  ++++ST H++ +LS S PPS+G F  G     +K
Sbjct: 130 DQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLG-DTMK 189

Query: 227 PMLSFLRETNSPLMVNPYPFFAC----TAENLDYALFRRNPGMFDSDLGILYANMLDAQL 286
            +L F    ++P M++PYP+FA     T + L + LF+ N G  DS  G  Y NM DAQ+
Sbjct: 190 ALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQV 249

Query: 287 DAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLM 346
           DAV+SA+ ++GF+D++IV+AETGWP  GD  +VG     A  Y GNL+ H+ S  GTPLM
Sbjct: 250 DAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLM 309

Query: 347 PNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVE 406
           P ++ +TY+F+L++ED K G   E+ FGLF+PD +  Y++G+L+ T    TP        
Sbjct: 310 PGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPAT------ 369

Query: 407 GPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNT 466
            P  TP+++          WC+PK   + + L  NI+Y CG G+DCGPIQ  G CF PNT
Sbjct: 370 -PTPTPKAAG--------SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNT 429

Query: 467 VRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
           V+AHAAYVMN Y+Q+   N ++CDF QT  LT  +PSYG C +
Sbjct: 430 VKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 455

BLAST of PI0009067 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-92
Identity = 182/474 (38.40%), Postives = 274/474 (57.81%), Query Frame = 0

Query: 47  LILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAF 106
           + +S L  +       IG+ YG  ADNLP P++V+  L+ +  I  VR++DA+ ++L+AF
Sbjct: 11  IFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSE-LIQHLNIKFVRIYDANIDVLKAF 70

Query: 107 AHTGISVSITIPN-DQIPHLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLL 166
           A+TGI + I +PN D +      +  + W+  NI PY P+T I  + VG EV    +   
Sbjct: 71  ANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-A 130

Query: 167 IANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLS 226
              ++PAM+++HTAL ++ LD++I+IS+ HSL ILS S PPS+  F + +    +KPML 
Sbjct: 131 TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLE 190

Query: 227 FLRETNSPLMVNPYPFFACTAEN----LDYALFRRNPGMFDSDLGILYANMLDAQLDAVY 286
           FL E  SP M++ YP++A         L+YALF  +  + D   G+LY+NM DAQLDA+Y
Sbjct: 191 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 250

Query: 287 SAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRT 346
            A+ ++ F+ + +++ E+GWPSKG P +    P+ A  YN NL+RHV+   GTP  P   
Sbjct: 251 FALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEE 310

Query: 347 FETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPIS 406
            + Y+F+LFNE+ KPG   ERN+G+F  + T VY +     T ++ TP    SP      
Sbjct: 311 IDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDF---TGENTTP---VSPTNSTTG 370

Query: 407 TPRSSSPSPV--------------SESKRWCLPKTEANVEALQRNIDYVCGLG-LDCGPI 466
           T  S S SP+                +K+WC+  ++A+V  LQ  +D+ CG G +DC  +
Sbjct: 371 TSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAV 430

Query: 467 QENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
           Q + PCF P+TV +HA+Y  N Y+Q +  +  DC F+        DPSYG C Y
Sbjct: 431 QPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of PI0009067 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.0e-90
Identity = 180/456 (39.47%), Postives = 258/456 (56.58%), Query Frame = 0

Query: 52  LIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGI 111
           L+ +   YGG +GV YG  AD+LP PSKV   L+    I  VR++D +  +L+AF +T I
Sbjct: 15  LLLLDCCYGGKVGVCYGRSADDLPTPSKVVQ-LIQQHNIKYVRIYDYNSQVLKAFGNTSI 74

Query: 112 SVSITIPNDQIPHLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLV 171
            + I +PN  +    +  +  + W+K ++ PY P T I  + VG E     +    + +V
Sbjct: 75  ELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVV 134

Query: 172 PAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRET 231
           PAMQ++ TAL +  L RRI++ST  SLGILS S PPS G F   Y  + ++PML FL E 
Sbjct: 135 PAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRPMLEFLAEN 194

Query: 232 NSPLMVNPYPFFACTAE----NLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKS 291
            SP M++ YP++A        +LDY LF  +  + D + G+LY NM DAQ+DA+Y A+ +
Sbjct: 195 KSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTA 254

Query: 292 LGFEDLDIVIAETGWPSKGDPTQVGVGPKE-AAYYNGNLMRHVVSGKGTPLMPNRTFETY 351
           L F  + I++ ETGWP+KG P +      + A  YN N++RHVV+ +GTP  P      Y
Sbjct: 255 LNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVY 314

Query: 352 IFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRS 411
           IF+LFNE+ K G   ERN+GLF PD T VY++        +      HS   G  S+  S
Sbjct: 315 IFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF------TGKSNGFHSNSSGTNSSGSS 374

Query: 412 SSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLG-LDCGPIQENGPCFAPNTVRAHAAY 471
           +S         WC+  ++A+   L+  +D+ CG G +DC  IQ + PCF P+T+ +HA++
Sbjct: 375 NS---------WCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASF 434

Query: 472 VMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
           V N+YFQ     D  C F   G     DPSY KC Y
Sbjct: 435 VFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIY 453

BLAST of PI0009067 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 4.6e-275
Identity = 464/489 (94.89%), Postives = 476/489 (97.34%), Query Frame = 0

Query: 13  QIHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVAD 72
           +IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLIL TLIQIQGV GGSIGVNYGTVAD
Sbjct: 11  KIHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVAD 70

Query: 73  NLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAE 132
           NLPPPSKVAAFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAE
Sbjct: 71  NLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAE 130

Query: 133 EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIS 192
           EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQIS
Sbjct: 131 EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQIS 190

Query: 193 TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA 252
           TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA
Sbjct: 191 TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA 250

Query: 253 LFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGV 312
           LFR NPG+FDSDLGILY NMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGV
Sbjct: 251 LFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGV 310

Query: 313 GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLT 372
           GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLT
Sbjct: 311 GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLT 370

Query: 373 PVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRN 432
           PVYEIGILRPTAQSATPRAH  PVEGPISTPRS S S VSESKRWC+PKTEA+VEALQRN
Sbjct: 371 PVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRN 430

Query: 433 IDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVD 492
           IDYVCGLGLDCGPIQENGPC+APNTVR HAAYVMNAYFQATEG +FDCDFDQTGALTTVD
Sbjct: 431 IDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVD 490

Query: 493 PSYGKCKYW 502
           PSYGKC+YW
Sbjct: 491 PSYGKCRYW 499

BLAST of PI0009067 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.2e-263
Identity = 448/472 (94.92%), Postives = 459/472 (97.25%), Query Frame = 0

Query: 22  MASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVA 81
           MASPPFTPSHHLLLPYFSTLPTFLLLIL TLIQIQGV GGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 82  AFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQP 141
           AFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 142 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 201
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 202 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMF 261
           NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFR NPG+F
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 262 DSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 321
           DSDLGILY NMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 322 GNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILR 381
           GNLMRHVV+GKGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLTPVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 382 PTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGL 441
           PTAQSATPRAH  PVEGPISTPRS S S VSESKRWC+PKTEA+VEALQRNIDYVCGLGL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 420

Query: 442 DCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDP 494
           DCGPIQENGPC+APNTVR HAAYVMNAYFQATEG +FDCDFDQTGALTTVDP
Sbjct: 421 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of PI0009067 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 9.0e-263
Identity = 450/488 (92.21%), Postives = 461/488 (94.47%), Query Frame = 0

Query: 14  IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADN 73
           I +  +HPMAS PFT SHHLLLPYFSTLPTFLLLIL TL QIQGV GGSIGVNYGTVADN
Sbjct: 13  IFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADN 72

Query: 74  LPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEE 133
           LPPPSKVAAFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAEE
Sbjct: 73  LPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEE 132

Query: 134 WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST 193
           WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST
Sbjct: 133 WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST 192

Query: 194 PHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYAL 253
           PHSLGILSNSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYAL
Sbjct: 193 PHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYAL 252

Query: 254 FRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG 313
           FR NPG+FDSDLGILY NMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG
Sbjct: 253 FRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG 312

Query: 314 PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTP 373
           PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDL+P
Sbjct: 313 PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSP 372

Query: 374 VYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNI 433
           VYEIGILRPTAQSATPRAH   VEGP       SPS VSESKRWCLPK+EA+ E LQRNI
Sbjct: 373 VYEIGILRPTAQSATPRAHQGQVEGP-------SPSTVSESKRWCLPKSEASEEGLQRNI 432

Query: 434 DYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDP 493
           DYVCGLGLDCGPI+ENG CFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTG LTTVDP
Sbjct: 433 DYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDP 492

Query: 494 SYGKCKYW 502
           SYGKCKYW
Sbjct: 493 SYGKCKYW 493

BLAST of PI0009067 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.7e-233
Identity = 397/469 (84.65%), Postives = 428/469 (91.26%), Query Frame = 0

Query: 33  LLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDR 92
           LL  Y S   T L+L+L TL+Q+Q   GGSIGVNYGT+ADNLP PSKVA FLLD+TIIDR
Sbjct: 10  LLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDR 69

Query: 93  VRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQPYIPATNIIRVL 152
           +RLFDA+P+ILRAFAHT ISV+IT+PNDQIP LVKPNFAEEWIKFN+QPY+PAT+IIR+L
Sbjct: 70  IRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRIL 129

Query: 153 VGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFR 212
           VGNEVLSTANK+LIANLVPAMQSLHTAL EASL+RRIQ+STPHSLGILSNS+PPST RFR
Sbjct: 130 VGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILSNSSPPSTARFR 189

Query: 213 QGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMFDSDLGILYANM 272
           QGYDTHVIKPMLSFLRETNSPLMVNPYPFF  TAE LDYALFR NPG++DSD GILY NM
Sbjct: 190 QGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNM 249

Query: 273 LDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGK 332
           LDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDP Q+GVGPKEAA YNGNL+RHVVSG 
Sbjct: 250 LDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGV 309

Query: 333 GTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAH 392
           GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDL+PVYEIGILRPTAQS+TPRAH
Sbjct: 310 GTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAH 369

Query: 393 HSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPC 452
           H    GP  TP+  SPSPV++ KRWCLPKTEA+ EALQRNIDYVCGLGLDCGPIQENGPC
Sbjct: 370 H----GPEPTPKGPSPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGPIQENGPC 429

Query: 453 FAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKYW 502
           F PNTVRAHAAYVMNAYFQAT+GND DCDF QTG LTTVDPSYGKCKYW
Sbjct: 430 FLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKYW 473

BLAST of PI0009067 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.6e-230
Identity = 402/503 (79.92%), Postives = 435/503 (86.48%), Query Frame = 0

Query: 1   MANATPKFTLFFQIHLHHLHPMASPPFT--PSHHLLLPYFSTLPTFLLLILSTLIQIQGV 60
           MANAT          L+ L  ++SP  +      LL  Y S   T LLL+L TL+Q+Q  
Sbjct: 24  MANAT----------LNSLFSLSSPMASSFALSRLLRAYTSIAVTLLLLLLPTLLQMQ-A 83

Query: 61  YGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIP 120
            GGSIGVNYGT+ADNLP PSKVA FLLD+TII R+RLFDA+P+ILRAFAHT ISV+IT+P
Sbjct: 84  KGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIGRIRLFDANPDILRAFAHTRISVTITVP 143

Query: 121 NDQIPHLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHT 180
           NDQIP LVKPNFAEEWIKFN+QPY+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHT
Sbjct: 144 NDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHT 203

Query: 181 ALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNP 240
           AL EASL+ RIQ+STPHSLGILSNS+PPST RFRQGYDTHVIKPMLSFL+ETNSPLMVNP
Sbjct: 204 ALSEASLNSRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVNP 263

Query: 241 YPFFACTAENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAE 300
           YPFF  TAE LDYALFR NPG++DSD GILY NMLDAQLDAVYSAMKSLGF DLDIVIAE
Sbjct: 264 YPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAE 323

Query: 301 TGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGP 360
           TGWPSKGDP Q+GVGPKEAA YNGNL+RHVVSG GTPLMPNRTFETYIFALFNEDLKPGP
Sbjct: 324 TGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGP 383

Query: 361 VGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWC 420
           VGERNFGLFEPDL PVYEIGILRPTAQS+TPRAHH    GP  TP+  SPSPV++ KRWC
Sbjct: 384 VGERNFGLFEPDLNPVYEIGILRPTAQSSTPRAHH----GPEPTPKGPSPSPVADGKRWC 443

Query: 421 LPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDF 480
           LPKTEA+ EALQRNIDYVCGLG DCGPIQ NGPCF PNTVRAHAAYVMNAYFQA +GND 
Sbjct: 444 LPKTEADSEALQRNIDYVCGLGFDCGPIQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDS 503

Query: 481 DCDFDQTGALTTVDPSYGKCKYW 502
           DCDF QTG LTTVDPSYGKCKYW
Sbjct: 504 DCDFQQTGDLTTVDPSYGKCKYW 511

BLAST of PI0009067 vs. NCBI nr
Match: XP_008437641.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo])

HSP 1 Score: 956.4 bits (2471), Expect = 9.5e-275
Identity = 464/489 (94.89%), Postives = 476/489 (97.34%), Query Frame = 0

Query: 13  QIHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVAD 72
           +IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLIL TLIQIQGV GGSIGVNYGTVAD
Sbjct: 11  KIHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVAD 70

Query: 73  NLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAE 132
           NLPPPSKVAAFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAE
Sbjct: 71  NLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAE 130

Query: 133 EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIS 192
           EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQIS
Sbjct: 131 EWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQIS 190

Query: 193 TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA 252
           TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA
Sbjct: 191 TPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYA 250

Query: 253 LFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGV 312
           LFR NPG+FDSDLGILY NMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGV
Sbjct: 251 LFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGV 310

Query: 313 GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLT 372
           GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLT
Sbjct: 311 GPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLT 370

Query: 373 PVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRN 432
           PVYEIGILRPTAQSATPRAH  PVEGPISTPRS S S VSESKRWC+PKTEA+VEALQRN
Sbjct: 371 PVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRN 430

Query: 433 IDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVD 492
           IDYVCGLGLDCGPIQENGPC+APNTVR HAAYVMNAYFQATEG +FDCDFDQTGALTTVD
Sbjct: 431 IDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVD 490

Query: 493 PSYGKCKYW 502
           PSYGKC+YW
Sbjct: 491 PSYGKCRYW 499

BLAST of PI0009067 vs. NCBI nr
Match: KAA0042530.1 (glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 448/472 (94.92%), Postives = 459/472 (97.25%), Query Frame = 0

Query: 22  MASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVA 81
           MASPPFTPSHHLLLPYFSTLPTFLLLIL TLIQIQGV GGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 82  AFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQP 141
           AFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 142 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 201
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL+EASLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 202 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMF 261
           NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFR NPG+F
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 262 DSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 321
           DSDLGILY NMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 322 GNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILR 381
           GNLMRHVV+GKGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLTPVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 382 PTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGL 441
           PTAQSATPRAH  PVEGPISTPRS S S VSESKRWC+PKTEA+VEALQRNIDYVCGLGL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 420

Query: 442 DCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDP 494
           DCGPIQENGPC+APNTVR HAAYVMNAYFQATEG +FDCDFDQTGALTTVDP
Sbjct: 421 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of PI0009067 vs. NCBI nr
Match: XP_011654592.1 (glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus])

HSP 1 Score: 915.6 bits (2365), Expect = 1.8e-262
Identity = 450/488 (92.21%), Postives = 461/488 (94.47%), Query Frame = 0

Query: 14  IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADN 73
           I +  +HPMAS PFT SHHLLLPYFSTLPTFLLLIL TL QIQGV GGSIGVNYGTVADN
Sbjct: 13  IFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADN 72

Query: 74  LPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEE 133
           LPPPSKVAAFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAEE
Sbjct: 73  LPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEE 132

Query: 134 WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST 193
           WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST
Sbjct: 133 WIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQIST 192

Query: 194 PHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYAL 253
           PHSLGILSNSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYAL
Sbjct: 193 PHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYAL 252

Query: 254 FRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG 313
           FR NPG+FDSDLGILY NMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG
Sbjct: 253 FRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVG 312

Query: 314 PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTP 373
           PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDL+P
Sbjct: 313 PKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSP 372

Query: 374 VYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNI 433
           VYEIGILRPTAQSATPRAH   VEGP       SPS VSESKRWCLPK+EA+ E LQRNI
Sbjct: 373 VYEIGILRPTAQSATPRAHQGQVEGP-------SPSTVSESKRWCLPKSEASEEGLQRNI 432

Query: 434 DYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDP 493
           DYVCGLGLDCGPI+ENG CFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTG LTTVDP
Sbjct: 433 DYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDP 492

Query: 494 SYGKCKYW 502
           SYGKCKYW
Sbjct: 493 SYGKCKYW 493

BLAST of PI0009067 vs. NCBI nr
Match: KAE8647796.1 (hypothetical protein Csa_000195 [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 1.8e-257
Identity = 447/497 (89.94%), Postives = 456/497 (91.75%), Query Frame = 0

Query: 22  MASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVA 81
           MAS PFT SHHLLLPYFSTLPTFLLLIL TL QIQGV GGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 82  AFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQP 141
           AFLLDNTIIDRVRLFDADP+ILRAFAHTGISVSITIPNDQIP LVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 142 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 201
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180

Query: 202 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMF 261
           NSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFR NPG+F
Sbjct: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240

Query: 262 DSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 321
           DSDLGILY NMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300

Query: 322 GNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILR 381
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360

Query: 382 PT-----------------AQSATPRAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEA 441
           PT                 AQSATPRAH   VEGP       SPS VSESKRWCLPK+EA
Sbjct: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGP-------SPSTVSESKRWCLPKSEA 420

Query: 442 NVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 501
           + E LQRNIDYVCGLGLDCGPI+ENG CFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ
Sbjct: 421 SEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 480

BLAST of PI0009067 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 882.1 bits (2278), Expect = 2.3e-252
Identity = 442/497 (88.93%), Postives = 461/497 (92.76%), Query Frame = 0

Query: 9   TLFFQIHLHHL--HPMASPPFTPSHHLLLPYFSTLPTFLLLILSTLIQIQGVYGGSIGVN 68
           TLF+  HLHH+   PMAS PFTPS HLL  + ST+ TFLLL L TLIQIQG   GSIGVN
Sbjct: 17  TLFY--HLHHITPPPMAS-PFTPS-HLLFVHISTVSTFLLL-LPTLIQIQGAKDGSIGVN 76

Query: 69  YGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLV 128
           YGT+ADNLPPPSKVAAFLLD+TIIDRVRLFDA+P+ILRAFAHTGISVSITIPNDQIP L 
Sbjct: 77  YGTLADNLPPPSKVAAFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLA 136

Query: 129 KPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLD 188
           KPNFAEEWIKFNI+PYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLD
Sbjct: 137 KPNFAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLD 196

Query: 189 RRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA 248
           RRIQISTPHSLGILSNS+PPSTGRFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFACTA
Sbjct: 197 RRIQISTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFACTA 256

Query: 249 ENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGD 308
           ENLDYALFR NPG+FDSD GILY NMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGD
Sbjct: 257 ENLDYALFRPNPGVFDSDSGILYTNMLDAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGD 316

Query: 309 PTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGL 368
             Q+GVGPKEAA YNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGL
Sbjct: 317 QAQIGVGPKEAADYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGL 376

Query: 369 FEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPR--SSSPSPVSESKRWCLPKTEA 428
           FEPDL+PVYEIGILRPTAQS+TP+AH  P+EG +STPR  S SPSPVSE KRWCLPKT A
Sbjct: 377 FEPDLSPVYEIGILRPTAQSSTPKAHQGPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGA 436

Query: 429 NVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 488
           + EALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGND DCDFDQ
Sbjct: 437 DSEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQ 496

Query: 489 TGALTTVDPSYGKCKYW 502
           TGALTTVDPSYGKCKYW
Sbjct: 497 TGALTTVDPSYGKCKYW 508

BLAST of PI0009067 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 599.7 bits (1545), Expect = 2.1e-171
Identity = 296/471 (62.85%), Postives = 360/471 (76.43%), Query Frame = 0

Query: 34  LLPYFSTLPTF----LLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTI 93
           +LP+FS L  F    +LLILST  +       SIGVNYGT+A+NLPPP +VA FLL +T+
Sbjct: 4   VLPFFS-LSMFTIVGVLLILSTGSE------ASIGVNYGTLANNLPPPQQVAEFLLHSTV 63

Query: 94  IDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQPYIPATNII 153
           I+R+RLFD DP IL+AFA+TGI+V++T+PNDQIPHL   + A++WI  +IQP+ P+TNII
Sbjct: 64  INRIRLFDTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNII 123

Query: 154 RVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTG 213
           R+LVGNEV+STA+ LLI  L+PAMQSLHTAL+ ASL RRIQISTPHSLG L+NSTPPS+ 
Sbjct: 124 RILVGNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSA 183

Query: 214 RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMFDSDLGILY 273
           +FR+GYD  V+KP+LSFLR T+SP +VNPYPFF  + E LD+ALFR NPG+FD    +LY
Sbjct: 184 KFRRGYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLY 243

Query: 274 ANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVV 333
            NMLDAQLD+VYSAM  LGF D++IVI E GWPS+GD  Q+GV    AA +N NL+  V 
Sbjct: 244 TNMLDAQLDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVD 303

Query: 334 SGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATP 393
           SG GTPLMPNRTFETYIFALFNE+LK GP  ERNFG+F  DLTP+Y+IGILRPT +S+  
Sbjct: 304 SGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSS-- 363

Query: 394 RAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQEN 453
                    P+  PR  SP   S SKRWC+ K  A   ALQRNIDYVCGLGLDC PI E 
Sbjct: 364 --------DPVYNPR--SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEG 423

Query: 454 GPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
           G C+ PNTV+AH+ Y MN Y+Q  E ++FDCDFD TG +TT+DPSYG C+Y
Sbjct: 424 GLCYLPNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of PI0009067 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 587.8 bits (1514), Expect = 8.2e-168
Identity = 292/465 (62.80%), Postives = 355/465 (76.34%), Query Frame = 0

Query: 34  LLPYFSTLPTF----LLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTI 93
           +LP+FS L  F    +LLILST  +       SIGVNYGT+A+NLPPP +VA FLL +T+
Sbjct: 4   VLPFFS-LSMFTIVGVLLILSTGSE------ASIGVNYGTLANNLPPPQQVAEFLLHSTV 63

Query: 94  IDRVRLFDADPNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQPYIPATNII 153
           I+R+RLFD DP IL+AFA+TGI+V++T+PNDQIPHL   + A++WI  +IQP+ P+TNII
Sbjct: 64  INRIRLFDTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNII 123

Query: 154 RVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTG 213
           R+LVGNEV+STA+ LLI  L+PAMQSLHTAL+ ASL RRIQISTPHSLG L+NSTPPS+ 
Sbjct: 124 RILVGNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSA 183

Query: 214 RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRRNPGMFDSDLGILY 273
           +FR+GYD  V+KP+LSFLR T+SP +VNPYPFF  + E LD+ALFR NPG+FD    +LY
Sbjct: 184 KFRRGYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLY 243

Query: 274 ANMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVV 333
            NMLDAQLD+VYSAM  LGF D++IVI E GWPS+GD  Q+GV    AA +N NL+  V 
Sbjct: 244 TNMLDAQLDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVD 303

Query: 334 SGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATP 393
           SG GTPLMPNRTFETYIFALFNE+LK GP  ERNFG+F  DLTP+Y+IGILRPT +S+  
Sbjct: 304 SGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSS-- 363

Query: 394 RAHHSPVEGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQEN 453
                    P+  PR  SP   S SKRWC+ K  A   ALQRNIDYVCGLGLDC PI E 
Sbjct: 364 --------DPVYNPR--SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEG 423

Query: 454 GPCFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPS 495
           G C+ PNTV+AH+ Y MN Y+Q  E ++FDCDFD TG +TT+DPS
Sbjct: 424 GLCYLPNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of PI0009067 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 422.5 bits (1085), Expect = 4.6e-118
Identity = 215/464 (46.34%), Postives = 297/464 (64.01%), Query Frame = 0

Query: 43  TFLLLILSTLIQIQGVYGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPN 102
           + L L+L  L  I      S IGVNYG ++DNLPPPS+ A  LL +T I +VRL++AD +
Sbjct: 4   SILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAK-LLQSTSIQKVRLYNADSS 63

Query: 103 ILRAFAHTGISVSITIPNDQIPHLVKP-NFAEEWIKFNIQPYIPATNIIRVLVGNEVLST 162
           I+ +   TGI + I + N  +P +    N A +WI  N+ P+ PA+NII + VGNEVL +
Sbjct: 64  IITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLS 123

Query: 163 ANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVI 222
            +  L+  L+PAMQ++  AL   SL  +I++ST H++ +L NS PPS G F   Y    +
Sbjct: 124 NDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG-L 183

Query: 223 KPMLSFLRETNSPLMVNPYPFFACTA----ENLDYALFRRNPGMFDSDLGILYANMLDAQ 282
           K +L FL +T SP  +NPYPFFA  +    E L + LF+ NPG  DS+ GI Y NM DAQ
Sbjct: 184 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQ 243

Query: 283 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 342
           +DAV+SA+KS+GFE +++++AETGWPS GD  +VG   + A  YNGNL+ H+ S  GTPL
Sbjct: 244 VDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPL 303

Query: 343 MPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPV 402
           MP ++ +TYIFALF+E+LKPGP  E++FGLF+PDL+  Y+IG+ + T+   +     SP 
Sbjct: 304 MPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQTS----QSPQ 363

Query: 403 EGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPN 462
            G ++            S  WC+PK +A  E LQ ++D+VCG G+DCGPI   G CF PN
Sbjct: 364 LGKVT------------SMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPN 423

Query: 463 TVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
            V +H AY MN YFQ +  N  DCDF +T  +T+ +PSY  C Y
Sbjct: 424 NVASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVY 449

BLAST of PI0009067 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 416.0 bits (1068), Expect = 4.3e-116
Identity = 210/443 (47.40%), Postives = 283/443 (63.88%), Query Frame = 0

Query: 63  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPNILRAFAHTGISVSITIPNDQI 122
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 123 PHLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 182
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 183 EASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 242
             SL  +I++ST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 243 FACTA----ENLDYALFRRNPGMFDSDLGILYANMLDAQLDAVYSAMKSLGFEDLDIVIA 302
           FA  +    E L + LF  N G  DS  GI Y NM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 303 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPG 362
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 363 PVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPVEGPISTPRSSSPSPVSESKRW 422
           P  ER FGLF+ DL+ VY++G+ + ++ S TP        G ++            S  W
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP-------SGKVT------------SSGW 385

Query: 423 CLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGND 482
           C+PK  A  E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +    
Sbjct: 386 CVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQP 445

Query: 483 FDCDFDQTGALTTVDPSYGKCKY 501
            DCDF +T  +T+ +PSY  C Y
Sbjct: 446 TDCDFSKTATVTSQNPSYNNCVY 447

BLAST of PI0009067 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.4e-111
Identity = 208/464 (44.83%), Postives = 296/464 (63.79%), Query Frame = 0

Query: 40  TLPTFLLLILSTLIQIQGVYGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDAD 99
           ++   LLL  +  + I  V   +IGVNYGT+  NLPPP++VA F+   T ID V++FD +
Sbjct: 7   SISLLLLLCAAVFLTIPAVI-SAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVN 66

Query: 100 PNILRAFAHTGISVSITIPNDQIPHLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLS 159
           P+ILRAFA TGISV +T+PN  IP L     A  W+  NI P+ P T I  + VGNE+L 
Sbjct: 67  PDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILL 126

Query: 160 TANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSN--STPPSTGRFRQGYDT 219
           T +  +I NL+PAM++L+ AL+ A + R ++++T HSL I++   +  PS+GRFR G+D 
Sbjct: 127 TGDNNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDK 186

Query: 220 HVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRR-NPGMFDSDLGILYANMLDAQ 279
            ++ P+L++ R T SP MVNPYP+F    +N+++A+FR     + D     +Y NM DA 
Sbjct: 187 GILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDAL 246

Query: 280 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 339
           +D+ YSAMK+LG+ D++IV+ ETGWPS  D       P  AA++N N+++    G+GTPL
Sbjct: 247 MDSTYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTPL 306

Query: 340 MPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHHSPV 399
           MPNR FETYIF LFNE+ KPGP  ERN+GLF  D +PVY++G+LR               
Sbjct: 307 MPNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR--------------- 366

Query: 400 EGPISTPRSSSPSPVSESKRWCLPKTEANVEALQRNIDYVCGLGLDCGPIQENGPCFAPN 459
            G     R + P+P +   +WC+ ++ A    LQ +I++VCG G+DC PIQ  G CF P+
Sbjct: 367 NGQGGGGRPALPAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPS 426

Query: 460 TVRAHAAYVMNAYFQATEGNDFDCDFDQTGALTTVDPSYGKCKY 501
           ++R HA++VMNAYFQ+    D  C+F  TG +   +PS G CKY
Sbjct: 427 SLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524093.4e-12650.56Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0696.0e-11547.40Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G861.4e-11143.41Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE51.4e-9238.40Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU93.0e-9039.47Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
A0A1S3AV364.6e-27594.89(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV... [more]
A0A5A7TGS65.2e-26394.92(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0KJS09.0e-26392.21(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3... [more]
A0A6J1H2P35.7e-23384.65(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 P... [more]
A0A6J1K7807.6e-23079.92(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=... [more]
Match NameE-valueIdentityDescription
XP_008437641.19.5e-27594.89PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo][more]
KAA0042530.11.1e-26294.92glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan e... [more]
XP_011654592.11.8e-26292.21glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus][more]
KAE8647796.11.8e-25789.94hypothetical protein Csa_000195 [Cucumis sativus][more]
XP_038906871.12.3e-25288.93glucan endo-1,3-beta-glucosidase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G24318.12.1e-17162.85O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.28.2e-16862.80O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.14.6e-11846.34O-Glycosyl hydrolases family 17 protein [more]
AT4G34480.14.3e-11647.40O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.12.4e-11144.83O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 416..500
e-value: 1.5E-40
score: 150.7
IPR012946X8 domainPFAMPF07983X8coord: 417..486
e-value: 3.0E-18
score: 66.3
NoneNo IPR availableGENE3D1.20.58.1040coord: 411..500
e-value: 1.5E-9
score: 40.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 61..384
e-value: 1.4E-117
score: 394.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 384..414
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..413
NoneNo IPR availablePANTHERPTHR32227:SF62O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 54..493
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 63..377
e-value: 5.8E-78
score: 262.6
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 292..305
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 54..493
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 63..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009067.1PI0009067.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds