PI0005707 (gene) Melon (PI 482460) v1

BLAST of PI0005707 vs. ExPASy Swiss-Prot
Match: Q9SJD4 (Long chain acyl-CoA synthetase 8 OS=Arabidopsis thaliana OX=3702 GN=LACS8 PE=1 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 1.6e-294
Identity = 485/702 (69.09%), Postives = 583/702 (83.05%), Query Frame = 0

Query: 30  YGKVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVDVGGDPGYAVRNVRVTELSQVPW 89
           +G  GI+G   + +L+ +LL  V    K+ K+RG+P+ VGG+ GY +R+ R  EL  VPW
Sbjct: 18  FGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRHARAPELVDVPW 77

Query: 90  KGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFD 149
           +GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGEY+WQ+YGEVF+
Sbjct: 78  EGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGEYKWQSYGEVFE 137

Query: 150 RACNFASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSL 209
           R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYASLGEEALI+SL
Sbjct: 138 RVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYASLGEEALIYSL 197

Query: 210 SETEVSTLICNSKQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEV 269
           +ET VSTLIC+SKQL+KL  I SSL TV+NIIY E+ G +       +     V S SEV
Sbjct: 198 NETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSMGDITVSSISEV 257

Query: 270 IKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDD 329
            KLG +N V P LPS + +AV+M+TSGSTGLPKGVMI+HGN+VATAAGVM V+PKLD +D
Sbjct: 258 EKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKND 317

Query: 330 VYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTA 389
            Y+AYLPLAHV EL AE V+ ++G A+GYGS +T+TDTS+K+KKGTKGDVS LKPTIMTA
Sbjct: 318 TYIAYLPLAHVFELEAEIVVFTSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKPTIMTA 377

Query: 390 VPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRN 449
           VPAILDRVR+GVLKKVEEKGG+ K LF  AY R+LAA++GSW GAWGLE++ W+ L+F+ 
Sbjct: 378 VPAILDRVREGVLKKVEEKGGMAKTLFDFAYKRRLAAVDGSWFGAWGLEKMLWDALVFKK 437

Query: 450 IRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGR 509
           IR+VLGG +RFML GGAPLS +SQRF NICMG+ IGQGYGLTET AGA FSEWDD +VGR
Sbjct: 438 IRAVLGGHIRFMLVGGAPLSPDSQRFINICMGSPIGQGYGLTETCAGATFSEWDDPAVGR 497

Query: 510 VGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKG 569
           VGPPLPC ++KLVSWEEGGYR +D PMPRGEIV+GG SVT GYF N EKT+EVYK DEKG
Sbjct: 498 VGPPLPCGYVKLVSWEEGGYRISDKPMPRGEIVVGGNSVTAGYFNNQEKTDEVYKVDEKG 557

Query: 570 IRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADP 629
            RWFYTGDIG FHPDGCLE+IDRKKDIVKLQHGEY+SLGK EAAL SS YVDN+MV ADP
Sbjct: 558 TRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSSNYVDNIMVHADP 617

Query: 630 FHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFE 689
            ++YCVA+VVP R ALE+WA+EAG+ + +F++LCE GE + EVQ+SL+K GK AKL+KFE
Sbjct: 618 INSYCVALVVPSRGALEKWAEEAGVKHSEFAELCEKGEAVKEVQQSLTKAGKAAKLEKFE 677

Query: 690 IPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           +P KI L  +PWTPESGLVTAALKIKREQ+K+KFKD+L +LY
Sbjct: 678 LPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSKLY 719

BLAST of PI0005707 vs. ExPASy Swiss-Prot
Match: Q9CAP8 (Long chain acyl-CoA synthetase 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LACS9 PE=1 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 3.3e-263
Identity = 434/690 (62.90%), Postives = 546/690 (79.13%), Query Frame = 0

Query: 42  GILIAILLPSVFLVKKRRKRRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQ 101
           G+++ + L  +    K+ K+RG+ VDVGG+PGYA+RN R TE     W+  +T+  LFE 
Sbjct: 7   GVIVPLALTFLVQKSKKEKKRGVVVDVGGEPGYAIRNHRFTEPVSSHWEHISTLPELFEI 66

Query: 102 SCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGF 161
           SC  HS   FLGTRKLIS+E  T+ DG+ FEKL+LG+YEW T+G+  +  C+FASGLV  
Sbjct: 67  SCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQI 126

Query: 162 GHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNS 221
           GH  + RVAI+A+T  EWFI+ QGC R+++TVVTIY+SLGEEAL HSL+ETEV+T+IC S
Sbjct: 127 GHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGS 186

Query: 222 KQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPS 281
           K+L+KLM+IS  L TV+ +I  +D     +F S +N S W   SF++V KLG ENPV P+
Sbjct: 187 KELKKLMDISQQLETVKRVICMDD-----EFPSDVN-SNWMATSFTDVQKLGRENPVDPN 246

Query: 282 LPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVL 341
            P S  +AV+MYTSGSTGLPKGVM++HGN++AT + VMT++P L   D+Y+AYLPLAH+L
Sbjct: 247 FPLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLGKRDIYMAYLPLAHIL 306

Query: 342 ELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGV 401
           ELAAE+V+ + G A+GYGSPLT+TDTS+KIKKGTKGDV+ LKPTIMTAVPAILDRVRDGV
Sbjct: 307 ELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTALKPTIMTAVPAILDRVRDGV 366

Query: 402 LKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFM 461
            KKV+ KGGL KKLF  AY R+L+A+ GSW GAWGLE+L W+VL+FR IR+VLGG++R++
Sbjct: 367 RKKVDAKGGLSKKLFDFAYARRLSAINGSWFGAWGLEKLLWDVLVFRKIRAVLGGQIRYL 426

Query: 462 LCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKL 521
           L GGAPLSG++QRF NIC+GA IGQGYGLTET AG  FSE++DTSVGRVG PLPC F+KL
Sbjct: 427 LSGGAPLSGDTQRFINICVGAPIGQGYGLTETCAGGTFSEFEDTSVGRVGAPLPCSFVKL 486

Query: 522 VSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAF 581
           V W EGGY T+D PMPRGEIVIGG+++T+GYFKN EKT EVYK DEKG+RWFYTGDIG F
Sbjct: 487 VDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKEVYKVDEKGMRWFYTGDIGRF 546

Query: 582 HPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPL 641
           HPDGCLEIIDRKKDIVKLQHGEY+SLGK EAAL  S YV+N+MV AD F++YCVA+VV  
Sbjct: 547 HPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVENIMVHADSFYSYCVALVVAS 606

Query: 642 RPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPW 701
           +  +E WA + GI++ +F +LC   + + EV  SL K  K ++L+KFEIP KI L   PW
Sbjct: 607 QHTVEGWASKQGIDFANFEELCTKEQAVKEVYASLVKAAKQSRLEKFEIPAKIKLLASPW 666

Query: 702 TPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           TPESGLVTAALK+KR+ ++ +F + L +LY
Sbjct: 667 TPESGLVTAALKLKRDVIRREFSEDLTKLY 690

BLAST of PI0005707 vs. ExPASy Swiss-Prot
Match: O95573 (Long-chain-fatty-acid--CoA ligase 3 OS=Homo sapiens OX=9606 GN=ACSL3 PE=1 SV=3)

HSP 1 Score: 586.3 bits (1510), Expect = 4.9e-166
Identity = 323/703 (45.95%), Postives = 446/703 (63.44%), Query Frame = 0

Query: 40  FIGILIAI-----LLPSVFLVKKRRKR--RGIPVDVGGDPGYAVRNVRVTE-LSQVPWKG 99
           FI  LI++      +P  F  + R+++  R     V   P  A R+V   + L+ V + G
Sbjct: 25  FIHFLISLYTILTYIPFYFFSESRQEKSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYPG 84

Query: 100 ATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRA 159
             T+  +F  +  K    + LGTR+++++E     +G+ F+K+ LG+Y W +Y +VF RA
Sbjct: 85  CDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRA 144

Query: 160 CNFASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSE 219
            NF +GL   G    + +AI+ ET AEW IA Q C   +  +VT+YA+LG  A++H+L+E
Sbjct: 145 FNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNE 204

Query: 220 TEVSTLICNSKQLR-KLMEISSSLTTVENIIYFEDAGYE-TDFISSINTSKWKVISFSEV 279
           TEV+ +I + + L+ KL +I S +  + +II  +      ++F   I       +  +  
Sbjct: 205 TEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVE-ALG 264

Query: 280 IKLGMEN-PVVPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSD 339
            K  MEN P    LPS   IAV+MYTSGSTGLPKGVMISH NI+A   G+   IP+L  +
Sbjct: 265 AKASMENQPHSKPLPS--DIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEE 324

Query: 340 DVYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMT 399
           DVY+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SSKIKKG+KGD S+LKPT+M 
Sbjct: 325 DVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMA 384

Query: 400 AVPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFR 459
           AVP I+DR+   V+ KV E     + LF +AYN ++  +      + G      +  +FR
Sbjct: 385 AVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI------SKGRNTPLCDSFVFR 444

Query: 460 NIRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVG 519
            +RS+LGG +R +LCGGAPLS  +QRF NIC    +GQGYGLTE+      SE  D + G
Sbjct: 445 KVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTG 504

Query: 520 RVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEK 579
           RVG PL CC IKL +WEEGGY  TD P PRGEI+IGG SVT+GY+KN  KT   + +DE 
Sbjct: 505 RVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDEN 564

Query: 580 GIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFAD 639
           G RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK EAAL +   VDN+  +A+
Sbjct: 565 GQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYAN 624

Query: 640 PFHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKF 699
            +H+Y +  VVP +  L E A++ G+  G + +LC + E  +EV K LS+    A L+KF
Sbjct: 625 SYHSYVIGFVVPNQKELTELARKKGLK-GTWEELCNSCEMENEVLKVLSEAAISASLEKF 684

Query: 700 EIPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           EIP KI L+P+PWTPE+GLVT A K+KR+++K  ++  ++++Y
Sbjct: 685 EIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMY 717

BLAST of PI0005707 vs. ExPASy Swiss-Prot
Match: Q5R668 (Long-chain-fatty-acid--CoA ligase 3 OS=Pongo abelii OX=9601 GN=ACSL3 PE=2 SV=2)

HSP 1 Score: 585.9 bits (1509), Expect = 6.4e-166
Identity = 323/703 (45.95%), Postives = 445/703 (63.30%), Query Frame = 0

Query: 40  FIGILIAI-----LLPSVFLVKKRRKR--RGIPVDVGGDPGYAVRNVRVTE-LSQVPWKG 99
           FI  LI++      +P  F  + R+++  R     V   P  A R+V   + L+ V + G
Sbjct: 25  FIHFLISLYTILTYIPFYFFSESRQEKSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYPG 84

Query: 100 ATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRA 159
             T+  +F  +  K    + LGTR+++++E     +G+ F+K+ LG+Y W +Y +VF RA
Sbjct: 85  CDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRA 144

Query: 160 CNFASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSE 219
            NF +GL   G    + +AI+ ET AEW IA Q C   +  +VT+YA+LG  A++H+L+E
Sbjct: 145 FNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNE 204

Query: 220 TEVSTLICNSKQLR-KLMEISSSLTTVENIIYFEDAGYE-TDFISSINTSKWKVISFSEV 279
           TEV+ +I + + L+ KL +I S +  + +II  +      ++F   I       +  +  
Sbjct: 205 TEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVE-ALG 264

Query: 280 IKLGMEN-PVVPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSD 339
            K  MEN P    LPS   IAV+MYTSGSTGLPKGVMISH NI+A   G+   IP+L  +
Sbjct: 265 AKASMENQPHSKPLPS--DIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEE 324

Query: 340 DVYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMT 399
           DVY+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SSKIKKG+KGD S+LKPT+M 
Sbjct: 325 DVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMA 384

Query: 400 AVPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFR 459
           AVP I+DR+   V+ KV E     + LF +AYN ++  +      + G      N  +FR
Sbjct: 385 AVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI------SKGRNTPLCNSFVFR 444

Query: 460 NIRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVG 519
            +RS+LGG +R +LCGGAPLS  +QRF NIC    +GQGYGLTE+      SE  D + G
Sbjct: 445 KVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTG 504

Query: 520 RVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEK 579
           RVG PL CC IKL +WEEGGY  TD P PRGEI+IGG  VT+GY+KN  KT   + +DE 
Sbjct: 505 RVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQIVTMGYYKNEAKTKADFFEDEN 564

Query: 580 GIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFAD 639
           G RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK EAAL +   VDN+  +A+
Sbjct: 565 GQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYAN 624

Query: 640 PFHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKF 699
            +H+Y +  VVP +  L E A++ G+  G + +LC + E  +EV K LS+    A L+KF
Sbjct: 625 SYHSYVIGFVVPNQKELTELARKKGLK-GTWEELCNSCEMENEVLKVLSEAAISASLEKF 684

Query: 700 EIPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           EIP KI L+P+PWTPE+GLVT A K+KR+++K  ++  ++++Y
Sbjct: 685 EIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMY 717

BLAST of PI0005707 vs. ExPASy Swiss-Prot
Match: Q63151 (Long-chain-fatty-acid--CoA ligase 3 OS=Rattus norvegicus OX=10116 GN=Acsl3 PE=1 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 3.2e-165
Identity = 319/703 (45.38%), Postives = 444/703 (63.16%), Query Frame = 0

Query: 40  FIGILIAIL--LPSVFLVKKRRKR----RGIPVDVGGDPGYAVRNVRVTELSQVPWKGAT 99
           FI  L  IL  +P  FL + ++++    +  PV    D  Y   N  +  L+ V + G  
Sbjct: 28  FIISLYTILTYIPFYFLCESKQEKPNHIKAKPVSSKPDSAYRSVN-SMDGLASVLYPGCD 87

Query: 100 TMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACN 159
           T+  +F  +  K    + LGTR+++++E     +G+ F+K+ LG Y W +Y +VF RA +
Sbjct: 88  TLDKVFMYAKNKFKDKRLLGTREILNEEDEIQPNGKVFKKVILGHYNWLSYEDVFIRALD 147

Query: 160 FASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSETE 219
           F +GL   G    + +AI+ ET AEW IA Q C   +  +VT+YA+LG  A++H L+ETE
Sbjct: 148 FGNGLQMLGQKPKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETE 207

Query: 220 VSTLICNSKQLR-KLMEISSSLTTVENIIYFEDAGYE-TDFISSINTSKWKVISFSEVIK 279
           V+ +I + + L+ KL +I S +  + +II  +      ++F   +      V + + V  
Sbjct: 208 VTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGV-----IVHTMAAVQA 267

Query: 280 LGMENPV---VPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSD 339
           LG++  V     S P    IAV+MYTSGSTG+PKGVMISH NI+A+  G+   IP+L  +
Sbjct: 268 LGVKADVDKKAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEE 327

Query: 340 DVYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMT 399
           DVY+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SSKIKKG+KGD SVLKPT+M 
Sbjct: 328 DVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMA 387

Query: 400 AVPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFR 459
           AVP I+DR+   V+ KV E     + LF +AYN ++  +      + G      +  +FR
Sbjct: 388 AVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQI------SKGCSTPLCDRFVFR 447

Query: 460 NIRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVG 519
           N+R +LGG +R +LCGGAPLS  +QRF NIC    +GQGYGLTE+      +E  D + G
Sbjct: 448 NVRRLLGGNIRVLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTG 507

Query: 520 RVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEK 579
           RVG PL CC IKL +WEEGGY  TD P PRGEI+IGG +VT+GY+KN  KT   + +DE 
Sbjct: 508 RVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKADFFEDEN 567

Query: 580 GIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFAD 639
           G RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK EAAL +   +DN+  +A+
Sbjct: 568 GQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYAN 627

Query: 640 PFHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKF 699
            +H+Y +  VVP +  L E A+  G N G + +LC + E  +EV K LS+    A L+KF
Sbjct: 628 SYHSYVIGFVVPNQKELTELARTKGFN-GTWEELCNSSEMENEVLKVLSEAAISASLEKF 687

Query: 700 EIPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           EIP KI L+PDPWTPE+GLVT A K+KR+++K  ++  ++++Y
Sbjct: 688 EIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIERMY 717

BLAST of PI0005707 vs. ExPASy TrEMBL
Match: A0A5A7UTI1 (Long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001400 PE=4 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 707/733 (96.45%), Postives = 721/733 (98.36%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLRKLMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGVMTVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKK-VEEKGGLVKKLFHIA 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKK VEEKGGLVKKLFHIA
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVVEEKGGLVKKLFHIA 445

Query: 421 YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNIC 480
           YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNIC
Sbjct: 446 YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNIC 505

Query: 481 MGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG 540
           MG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG
Sbjct: 506 MGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG 565

Query: 541 EIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKL 600
           EIVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKL
Sbjct: 566 EIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKL 625

Query: 601 QHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDF 660
           QHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+F
Sbjct: 626 QHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEF 685

Query: 661 SQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQV 720
           SQLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQV
Sbjct: 686 SQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQV 745

Query: 721 KAKFKDQLQQLYE 733
           KAKFKDQLQQLY+
Sbjct: 746 KAKFKDQLQQLYK 758

BLAST of PI0005707 vs. ExPASy TrEMBL
Match: A0A1S3CGI4 (long chain acyl-CoA synthetase 8 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 1   MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 60

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 61  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 120

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 121 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 180

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 181 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 240

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 241 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 300

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 301 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 421 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 480

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 481 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 541 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 601 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 660

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 661 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 720

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 721 AKFKDQLQQLYK 732

BLAST of PI0005707 vs. ExPASy TrEMBL
Match: A0A1S4E3L6 (long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 445

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 446 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 505

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 506 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 565

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 566 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 625

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 626 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 685

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 686 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 745

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 746 AKFKDQLQQLYK 757

BLAST of PI0005707 vs. ExPASy TrEMBL
Match: A0A1S4E3L7 (long chain acyl-CoA synthetase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 11  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 70

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 71  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 130

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 131 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 190

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 191 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 250

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 251 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 310

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 311 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 370

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 371 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 430

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 431 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 490

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 491 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 550

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 551 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 610

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 611 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 670

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 671 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 730

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 731 AKFKDQLQQLYK 742

BLAST of PI0005707 vs. ExPASy TrEMBL
Match: A0A5D3D6X5 (Long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00280 PE=4 SV=1)

HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLRKLMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGVMTVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 445

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 446 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 505

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 506 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 565

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNE   DDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 566 IVIGGTSVAVGYFKNPEKTNE---DDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 625

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 626 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 685

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 686 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 745

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 746 AKFKDQLQQLYK 754

BLAST of PI0005707 vs. NCBI nr
Match: KAA0059212.1 (long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 707/733 (96.45%), Postives = 721/733 (98.36%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLRKLMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGVMTVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKK-VEEKGGLVKKLFHIA 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKK VEEKGGLVKKLFHIA
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVVEEKGGLVKKLFHIA 445

Query: 421 YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNIC 480
           YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNIC
Sbjct: 446 YNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNIC 505

Query: 481 MGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG 540
           MG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG
Sbjct: 506 MGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRG 565

Query: 541 EIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKL 600
           EIVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKL
Sbjct: 566 EIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKL 625

Query: 601 QHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDF 660
           QHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+F
Sbjct: 626 QHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEF 685

Query: 661 SQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQV 720
           SQLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQV
Sbjct: 686 SQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQV 745

Query: 721 KAKFKDQLQQLYE 733
           KAKFKDQLQQLY+
Sbjct: 746 KAKFKDQLQQLYK 758

BLAST of PI0005707 vs. NCBI nr
Match: XP_016902819.1 (PREDICTED: long chain acyl-CoA synthetase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 11  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 70

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 71  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 130

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 131 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 190

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 191 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 250

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 251 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 310

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 311 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 370

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 371 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 430

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 431 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 490

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 491 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 550

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 551 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 610

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 611 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 670

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 671 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 730

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 731 AKFKDQLQQLYK 742

BLAST of PI0005707 vs. NCBI nr
Match: XP_016902818.1 (PREDICTED: long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 445

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 446 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 505

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 506 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 565

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 566 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 625

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 626 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 685

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 686 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 745

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 746 AKFKDQLQQLYK 757

BLAST of PI0005707 vs. NCBI nr
Match: XP_008462036.1 (PREDICTED: long chain acyl-CoA synthetase 8 isoform X3 [Cucumis melo] >XP_008462038.1 PREDICTED: long chain acyl-CoA synthetase 8 isoform X3 [Cucumis melo])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 1   MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 60

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 61  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 120

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 121 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 180

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLR LMEISSSLTTV NI
Sbjct: 181 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 240

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 241 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 300

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGV TVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 301 PKGVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLA LEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 421 NRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 480

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 481 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNEVYKDDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 541 IVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 601 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 660

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 661 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 720

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 721 AKFKDQLQQLYK 732

BLAST of PI0005707 vs. NCBI nr
Match: TYK19308.1 (long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 704/732 (96.17%), Postives = 718/732 (98.09%), Query Frame = 0

Query: 1   MTGDSIENIRNSKILENLHAGDYSLVWRGYGKVGIVGTIFIGILIAILLPSVFLVKKRRK 60
           MTG+SIE+IRNSKI+ENLHAGDYSLVWRGYG VGIVGTIFIGILIAILLPSVFLVKKRRK
Sbjct: 26  MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 85

Query: 61  RRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120
           RRGIPVDVGGDPGYAVRN RVTELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 86  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 145

Query: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDSRVAIYAETCAEWF 180
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVDSRVAIYAETCAEWF
Sbjct: 146 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 205

Query: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNSKQLRKLMEISSSLTTVENI 240
           +AFQGCIRQDITVVTIYASLGEEALIHSL+ETEVSTLICNSKQLRKLMEISSSLTTV NI
Sbjct: 206 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNI 265

Query: 241 IYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 300
           IYFEDAGYETDFISS NTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 266 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 325

Query: 301 PKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 360
           PKGVMISHGNIVAT AGVMTVIPKL+SDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS
Sbjct: 326 PKGVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEAVILSAGLAMGYGS 385

Query: 361 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 420
           PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY
Sbjct: 386 PLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIAY 445

Query: 421 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESQRFFNICM 480
           NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGES RFFNICM
Sbjct: 446 NRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAPLSGESLRFFNICM 505

Query: 481 GATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 540
           G+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE
Sbjct: 506 GSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDIPMPRGE 565

Query: 541 IVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 600
           IVIGGTSV VGYFKNPEKTNE   DDEKG RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ
Sbjct: 566 IVIGGTSVAVGYFKNPEKTNE---DDEKGTRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQ 625

Query: 601 HGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPLRPALEEWAKEAGINYGDFS 660
           HGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALEEWAKEAGINYG+FS
Sbjct: 626 HGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEEWAKEAGINYGEFS 685

Query: 661 QLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPWTPESGLVTAALKIKREQVK 720
           QLCENGE+ISEVQ+SLSKVGKDAKLQKFEIPEKI+LAPDPWTPESGLVTAALKIKREQVK
Sbjct: 686 QLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGLVTAALKIKREQVK 745

Query: 721 AKFKDQLQQLYE 733
           AKFKDQLQQLY+
Sbjct: 746 AKFKDQLQQLYK 754

BLAST of PI0005707 vs. TAIR 10
Match: AT2G04350.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1013.1 bits (2618), Expect = 1.2e-295
Identity = 485/702 (69.09%), Postives = 583/702 (83.05%), Query Frame = 0

Query: 30  YGKVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVDVGGDPGYAVRNVRVTELSQVPW 89
           +G  GI+G   + +L+ +LL  V    K+ K+RG+P+ VGG+ GY +R+ R  EL  VPW
Sbjct: 18  FGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRHARAPELVDVPW 77

Query: 90  KGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFD 149
           +GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGEY+WQ+YGEVF+
Sbjct: 78  EGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGEYKWQSYGEVFE 137

Query: 150 RACNFASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSL 209
           R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYASLGEEALI+SL
Sbjct: 138 RVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYASLGEEALIYSL 197

Query: 210 SETEVSTLICNSKQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEV 269
           +ET VSTLIC+SKQL+KL  I SSL TV+NIIY E+ G +       +     V S SEV
Sbjct: 198 NETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSMGDITVSSISEV 257

Query: 270 IKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDD 329
            KLG +N V P LPS + +AV+M+TSGSTGLPKGVMI+HGN+VATAAGVM V+PKLD +D
Sbjct: 258 EKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKND 317

Query: 330 VYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTA 389
            Y+AYLPLAHV EL AE V+ ++G A+GYGS +T+TDTS+K+KKGTKGDVS LKPTIMTA
Sbjct: 318 TYIAYLPLAHVFELEAEIVVFTSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKPTIMTA 377

Query: 390 VPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRN 449
           VPAILDRVR+GVLKKVEEKGG+ K LF  AY R+LAA++GSW GAWGLE++ W+ L+F+ 
Sbjct: 378 VPAILDRVREGVLKKVEEKGGMAKTLFDFAYKRRLAAVDGSWFGAWGLEKMLWDALVFKK 437

Query: 450 IRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGR 509
           IR+VLGG +RFML GGAPLS +SQRF NICMG+ IGQGYGLTET AGA FSEWDD +VGR
Sbjct: 438 IRAVLGGHIRFMLVGGAPLSPDSQRFINICMGSPIGQGYGLTETCAGATFSEWDDPAVGR 497

Query: 510 VGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKG 569
           VGPPLPC ++KLVSWEEGGYR +D PMPRGEIV+GG SVT GYF N EKT+EVYK DEKG
Sbjct: 498 VGPPLPCGYVKLVSWEEGGYRISDKPMPRGEIVVGGNSVTAGYFNNQEKTDEVYKVDEKG 557

Query: 570 IRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADP 629
            RWFYTGDIG FHPDGCLE+IDRKKDIVKLQHGEY+SLGK EAAL SS YVDN+MV ADP
Sbjct: 558 TRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSSNYVDNIMVHADP 617

Query: 630 FHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFE 689
            ++YCVA+VVP R ALE+WA+EAG+ + +F++LCE GE + EVQ+SL+K GK AKL+KFE
Sbjct: 618 INSYCVALVVPSRGALEKWAEEAGVKHSEFAELCEKGEAVKEVQQSLTKAGKAAKLEKFE 677

Query: 690 IPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           +P KI L  +PWTPESGLVTAALKIKREQ+K+KFKD+L +LY
Sbjct: 678 LPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSKLY 719

BLAST of PI0005707 vs. TAIR 10
Match: AT2G04350.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1013.1 bits (2618), Expect = 1.2e-295
Identity = 485/702 (69.09%), Postives = 583/702 (83.05%), Query Frame = 0

Query: 30  YGKVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVDVGGDPGYAVRNVRVTELSQVPW 89
           +G  GI+G   + +L+ +LL  V    K+ K+RG+P+ VGG+ GY +R+ R  EL  VPW
Sbjct: 18  FGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRHARAPELVDVPW 77

Query: 90  KGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFD 149
           +GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGEY+WQ+YGEVF+
Sbjct: 78  EGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGEYKWQSYGEVFE 137

Query: 150 RACNFASGLVGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSL 209
           R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYASLGEEALI+SL
Sbjct: 138 RVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYASLGEEALIYSL 197

Query: 210 SETEVSTLICNSKQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEV 269
           +ET VSTLIC+SKQL+KL  I SSL TV+NIIY E+ G +       +     V S SEV
Sbjct: 198 NETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSMGDITVSSISEV 257

Query: 270 IKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDD 329
            KLG +N V P LPS + +AV+M+TSGSTGLPKGVMI+HGN+VATAAGVM V+PKLD +D
Sbjct: 258 EKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKND 317

Query: 330 VYLAYLPLAHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTA 389
            Y+AYLPLAHV EL AE V+ ++G A+GYGS +T+TDTS+K+KKGTKGDVS LKPTIMTA
Sbjct: 318 TYIAYLPLAHVFELEAEIVVFTSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKPTIMTA 377

Query: 390 VPAILDRVRDGVLKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRN 449
           VPAILDRVR+GVLKKVEEKGG+ K LF  AY R+LAA++GSW GAWGLE++ W+ L+F+ 
Sbjct: 378 VPAILDRVREGVLKKVEEKGGMAKTLFDFAYKRRLAAVDGSWFGAWGLEKMLWDALVFKK 437

Query: 450 IRSVLGGRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGR 509
           IR+VLGG +RFML GGAPLS +SQRF NICMG+ IGQGYGLTET AGA FSEWDD +VGR
Sbjct: 438 IRAVLGGHIRFMLVGGAPLSPDSQRFINICMGSPIGQGYGLTETCAGATFSEWDDPAVGR 497

Query: 510 VGPPLPCCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKG 569
           VGPPLPC ++KLVSWEEGGYR +D PMPRGEIV+GG SVT GYF N EKT+EVYK DEKG
Sbjct: 498 VGPPLPCGYVKLVSWEEGGYRISDKPMPRGEIVVGGNSVTAGYFNNQEKTDEVYKVDEKG 557

Query: 570 IRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADP 629
            RWFYTGDIG FHPDGCLE+IDRKKDIVKLQHGEY+SLGK EAAL SS YVDN+MV ADP
Sbjct: 558 TRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSSNYVDNIMVHADP 617

Query: 630 FHNYCVAVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFE 689
            ++YCVA+VVP R ALE+WA+EAG+ + +F++LCE GE + EVQ+SL+K GK AKL+KFE
Sbjct: 618 INSYCVALVVPSRGALEKWAEEAGVKHSEFAELCEKGEAVKEVQQSLTKAGKAAKLEKFE 677

Query: 690 IPEKIMLAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           +P KI L  +PWTPESGLVTAALKIKREQ+K+KFKD+L +LY
Sbjct: 678 LPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSKLY 719

BLAST of PI0005707 vs. TAIR 10
Match: AT1G77590.1 (long chain acyl-CoA synthetase 9 )

HSP 1 Score: 909.1 bits (2348), Expect = 2.4e-264
Identity = 434/690 (62.90%), Postives = 546/690 (79.13%), Query Frame = 0

Query: 42  GILIAILLPSVFLVKKRRKRRGIPVDVGGDPGYAVRNVRVTELSQVPWKGATTMAALFEQ 101
           G+++ + L  +    K+ K+RG+ VDVGG+PGYA+RN R TE     W+  +T+  LFE 
Sbjct: 7   GVIVPLALTFLVQKSKKEKKRGVVVDVGGEPGYAIRNHRFTEPVSSHWEHISTLPELFEI 66

Query: 102 SCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGF 161
           SC  HS   FLGTRKLIS+E  T+ DG+ FEKL+LG+YEW T+G+  +  C+FASGLV  
Sbjct: 67  SCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQI 126

Query: 162 GHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLICNS 221
           GH  + RVAI+A+T  EWFI+ QGC R+++TVVTIY+SLGEEAL HSL+ETEV+T+IC S
Sbjct: 127 GHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGS 186

Query: 222 KQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPVVPS 281
           K+L+KLM+IS  L TV+ +I  +D     +F S +N S W   SF++V KLG ENPV P+
Sbjct: 187 KELKKLMDISQQLETVKRVICMDD-----EFPSDVN-SNWMATSFTDVQKLGRENPVDPN 246

Query: 282 LPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHVL 341
            P S  +AV+MYTSGSTGLPKGVM++HGN++AT + VMT++P L   D+Y+AYLPLAH+L
Sbjct: 247 FPLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLGKRDIYMAYLPLAHIL 306

Query: 342 ELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGV 401
           ELAAE+V+ + G A+GYGSPLT+TDTS+KIKKGTKGDV+ LKPTIMTAVPAILDRVRDGV
Sbjct: 307 ELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTALKPTIMTAVPAILDRVRDGV 366

Query: 402 LKKVEEKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFM 461
            KKV+ KGGL KKLF  AY R+L+A+ GSW GAWGLE+L W+VL+FR IR+VLGG++R++
Sbjct: 367 RKKVDAKGGLSKKLFDFAYARRLSAINGSWFGAWGLEKLLWDVLVFRKIRAVLGGQIRYL 426

Query: 462 LCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKL 521
           L GGAPLSG++QRF NIC+GA IGQGYGLTET AG  FSE++DTSVGRVG PLPC F+KL
Sbjct: 427 LSGGAPLSGDTQRFINICVGAPIGQGYGLTETCAGGTFSEFEDTSVGRVGAPLPCSFVKL 486

Query: 522 VSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYTGDIGAF 581
           V W EGGY T+D PMPRGEIVIGG+++T+GYFKN EKT EVYK DEKG+RWFYTGDIG F
Sbjct: 487 VDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKEVYKVDEKGMRWFYTGDIGRF 546

Query: 582 HPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCVAVVVPL 641
           HPDGCLEIIDRKKDIVKLQHGEY+SLGK EAAL  S YV+N+MV AD F++YCVA+VV  
Sbjct: 547 HPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVENIMVHADSFYSYCVALVVAS 606

Query: 642 RPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIMLAPDPW 701
           +  +E WA + GI++ +F +LC   + + EV  SL K  K ++L+KFEIP KI L   PW
Sbjct: 607 QHTVEGWASKQGIDFANFEELCTKEQAVKEVYASLVKAAKQSRLEKFEIPAKIKLLASPW 666

Query: 702 TPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           TPESGLVTAALK+KR+ ++ +F + L +LY
Sbjct: 667 TPESGLVTAALKLKRDVIRREFSEDLTKLY 690

BLAST of PI0005707 vs. TAIR 10
Match: AT3G05970.1 (long-chain acyl-CoA synthetase 6 )

HSP 1 Score: 374.8 bits (961), Expect = 1.6e-103
Identity = 221/637 (34.69%), Postives = 332/637 (52.12%), Query Frame = 0

Query: 99  FEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGL 158
           FE +       K+LGTR  +        DG       +G+Y+W TYGE         SGL
Sbjct: 90  FEHAVHDFRDYKYLGTRVRV--------DG------TVGDYKWMTYGEAGTARTALGSGL 149

Query: 159 VGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLI 218
           V  G  + S V IY     EW I    C       V +Y +LG +A+   ++   V  + 
Sbjct: 150 VHHGIPMGSSVGIYFINRPEWLIVDHACSSYSYVSVPLYDTLGPDAVKFIVNHATVQAIF 209

Query: 219 CNSKQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPV 278
           C ++ L  L+   S + +V  ++     G      S  ++S  KV+S+S ++  G  NP 
Sbjct: 210 CVAETLNSLLSCLSEMPSVRLVVVV--GGLIESLPSLPSSSGVKVVSYSVLLNQGRSNPQ 269

Query: 279 VPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLA 338
               P    +A + YTSG+TG PKGV+++H N++A  AG    + K  S DVY++YLPLA
Sbjct: 270 RFFPPKPDDVATICYTSGTTGTPKGVVLTHANLIANVAGSSFSV-KFFSSDVYISYLPLA 329

Query: 339 HVLELAAEAVILSAGLAMGY--GSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDR 398
           H+ E A + + +  G+A+G+  G  + + D           D++ L+PT+ ++VP + +R
Sbjct: 330 HIYERANQILTVYFGVAVGFYQGDNMKLLD-----------DLAALRPTVFSSVPRLYNR 389

Query: 399 VRDGVLKKVEEKGGLVKKLFHIAYN-RQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLG 458
           +  G++  V+  GGL ++LF+ AYN ++ A L G            W+ L+F  I+  LG
Sbjct: 390 IYAGIINAVKTSGGLKERLFNAAYNAKKQALLNGKSASP------IWDRLVFNKIKDRLG 449

Query: 459 GRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLP 518
           GR+RFM  G +PLS E   F  +C G  + +GYG+TET    +  +  D   G VG P P
Sbjct: 450 GRVRFMTSGASPLSPEVMEFLKVCFGGRVTEGYGMTETSCVISGMDEGDNLTGHVGSPNP 509

Query: 519 CCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYT 578
            C +KLV   E  Y + D P PRGEI + G  +  GY+K+  +T EV  +D     W +T
Sbjct: 510 ACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEIQTKEVIDED----GWLHT 569

Query: 579 GDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCV 638
           GDIG + P G L+IIDRKK+I KL  GEYI+  K E      K+V    ++ D F++  V
Sbjct: 570 GDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYAKCKFVGQCFIYGDSFNSSLV 629

Query: 639 AVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIM 698
           AVV      L+ WA   GI  GD  +LC N    + V   +  VG++A+L+ FE  + + 
Sbjct: 630 AVVSVDPDVLKSWAASEGIKGGDLRELCNNPRVKAAVLSDMDTVGREAQLRGFEFAKAVT 688

Query: 699 LAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLYE 733
           L  +P+T E+GL+T   KIKR Q K  F + +  +Y+
Sbjct: 690 LVLEPFTLENGLLTPTFKIKRPQAKEYFAEAITNMYK 688

BLAST of PI0005707 vs. TAIR 10
Match: AT5G27600.1 (long-chain acyl-CoA synthetase 7 )

HSP 1 Score: 367.1 bits (941), Expect = 3.3e-101
Identity = 214/636 (33.65%), Postives = 331/636 (52.04%), Query Frame = 0

Query: 99  FEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGL 158
           F  +   +++NK+LGTR          SDG       +GEY W TYGE         SGL
Sbjct: 90  FVHAVETYAENKYLGTR--------VRSDG------TIGEYSWMTYGEAASERQAIGSGL 149

Query: 159 VGFGHDVDSRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLSETEVSTLI 218
           +  G +    V +Y     EW +    C       V +Y +LG +A+   ++   +  + 
Sbjct: 150 LFHGVNQGDCVGLYFINRPEWLVVDHACAAYSFVSVPLYDTLGPDAVKFVVNHANLQAIF 209

Query: 219 CNSKQLRKLMEISSSLTTVENIIYFEDAGYETDFISSINTSKWKVISFSEVIKLGMENPV 278
           C  + L  L+   + + ++  I+     G +    S    +   ++S+ +++  G  +  
Sbjct: 210 CVPQTLNILLSFLAEIPSIRLIVVV--GGADEHLPSLPRGTGVTIVSYQKLLSQGRSSLH 269

Query: 279 VPSLPSSHSIAVVMYTSGSTGLPKGVMISHGNIVATAAGVMTVIPKLDSDDVYLAYLPLA 338
             S P    IA + YTSG+TG PKGV+++HGN++A  AG  +V  +    DVY++YLPLA
Sbjct: 270 PFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAG-SSVEAEFFPSDVYISYLPLA 329

Query: 339 HVLELAAEAVILSAGLAMGY--GSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDR 398
           H+ E A + + +  G+A+G+  G    + D           D +VL+PTI  +VP + +R
Sbjct: 330 HIYERANQIMGVYGGVAVGFYQGDVFKLMD-----------DFAVLRPTIFCSVPRLYNR 389

Query: 399 VRDGVLKKVEEKGGLVKKLFHIAYN-RQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLG 458
           + DG+   V+  G + K+LF IAYN ++ A + G    A      FW+ L+F  I+  LG
Sbjct: 390 IYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSA------FWDKLVFNKIKEKLG 449

Query: 459 GRLRFMLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLP 518
           GR+RFM  G +PLS +   F  IC G ++ +GYG+TET    +  +  D   G VG P P
Sbjct: 450 GRVRFMGSGASPLSPDVMDFLRICFGCSVREGYGMTETSCVISAMDDGDNLSGHVGSPNP 509

Query: 519 CCFIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVTVGYFKNPEKTNEVYKDDEKGIRWFYT 578
            C +KLV   E  Y + D P PRGEI + G  +  GY+K+ E+T E+   D     W +T
Sbjct: 510 ACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKDEEQTREILDGD----GWLHT 569

Query: 579 GDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVFADPFHNYCV 638
           GDIG + P G L+IIDRKK+I KL  GEYI+  K E      ++V    +  D F++  V
Sbjct: 570 GDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYTKCRFVSQCFIHGDSFNSSLV 629

Query: 639 AVVVPLRPALEEWAKEAGINYGDFSQLCENGETISEVQKSLSKVGKDAKLQKFEIPEKIM 698
           A+V      +++WA   GI Y    QLC +      V   +  +G++A+L+ FE  + + 
Sbjct: 630 AIVSVDPEVMKDWAASEGIKYEHLGQLCNDPRVRKTVLAEMDDLGREAQLRGFEFAKAVT 687

Query: 699 LAPDPWTPESGLVTAALKIKREQVKAKFKDQLQQLY 732
           L P+P+T E+GL+T   KIKR Q KA F + + ++Y
Sbjct: 690 LVPEPFTLENGLLTPTFKIKRPQAKAYFAEAISKMY 687