PI0003608 (gene) Melon (PI 482460) v1

Overview


Name	PI0003608
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerases II and IV subunit 5A
Location	chr01: 22817615 .. 22821705 (-)
RNA-Seq Expression	PI0003608
Synteny	PI0003608

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACAAAATAAAAAATCCATTAAATCGATTATTTTTAAAAAGTTATTTTAATCAAATTAATTAATAGTCTAAGTTTAAATTCAAATAAAATCAGAGTAAGCCCTAATTTCTCGTTCCACCATCTTCTTCTTCTTCTTCTTCACGCCGGCTTCTCCGCTTCAATTTCAGCAGCTCAGTGACGCCCGTGTTCTACTTAGTGGGTTTTCCTGATTTCTTGTTGTTACTGGTAATTATCTCAATTTTCATCTATGCCTTTTTTTTTTTCTTTCTATAAATCGAAAAACTCCTTTTTGAACTTGTCTGATGAAAGAAAGAAGCAATACCCAGCGTCAAACAGTGAACTAATCGTTGATTTTGAACTTTTTTTTAGTTAATTACAAGTTACCAACTTGCTTTTCACCAATTACTCAGGTGGTGGTAGTGGTATAGCTCGAAGTTTTATAGTTCCATAATCCTTAAGGCCTTCATCTCCTCAAATGAAAGTTTCCTTTTTTATAAAAAAGAAAATTATATGTAAGTGTCTGGACCAGCTTACATCCAGCCCAACTAATTTCAGACGGGAGAACTTCTGGTGTCGAAGGAAACCTGTCGATATTAAATCCCAAATAGGTGAACAGCAACACTGTGGAAAGTTATGTTTTCTTTAGAGAAAATAGTTTGTGTAAAAAATATTGGTTAAGTAGTTAAAAGAATAATTCTTTTTTATCTTAGTAAAGGGCCTAGAGGTAGGCGAAGGCATGTTGGAGACTGCAAAAACGAATGATCTCTGTTCCGTTGATTCCCAGCTAGTGTGTTTTGTGTAGAAAGGACTTGGAAGAAAGGCTGCCCTTGTTTTTCCTATGCCTGTTTGATGCTTCATTTTTATTTTTATTTTATTTTTGATAAGAAACAGATTTCATAGACGAATGAAATTTACGTTTGCTGCTTCATTTTGATCCAGTCTCTCTTAAATATTTTACTTAGTCTGGGTGTTTCCAAGATTTGTTCATACTTCAGACAGAGGCTTTCTATCAGCTCATTTATGGAACCCTCTTAGAATTTTGTGGTATAATTAGGCGTTGGCTCTTCTGTGGAGAATTTGGTGTAAAAGAAATAGTACTTTTCTAGGAAGTGGCTGTACTCTTGTTGTATACTGGGAAAAAGGTGGTTGTCTACTTGGTATTAGTTGGTAGTTACAATCTTTAGCTTTTGTAATTATGGTTTATCTAAAATTTTTTGTCAACTAGGAGTTCTTTTTGTATCCCCCCTGGGCTTGTAGGGGATATCTCATTTCCTTTTTTATGTGTCTCCCTCTTATTAATGAGATTGTTTACTTATTTACTCATTCCTATATATATAAAAAGAAGTATATTACTAGAGGTAATGAGTTCAAGCCACAATAGTCACCCACCCGAGATTTAATGAGTGTTCTTTACAACCAAATCTAACAGGGTCAGGATGTTTACCTATTAGAAGAGTCAAAGCGTGCATGACTTGACCTGAACACTCATGAACATAGAAAAAGAAGGATAAAGTTCAAATCAGGATGGTCAATTAAGGATATTAAAATTTCTTTTAAATTTTTTTAATTCAAAATCACCATGGCTAAGCTGTTGACTTGTATGGCGGGTTGAGTTGTACATTCGTTGATCCAAACACCTATAGTTGTTTAAAACGATAATAATAGTAAAAGAAGGATGTTTATATGTTATGTACACATGTTTGAAAACTGTTGTTGCAGGTCTATGCTTTTCCAGCAGGATTTTATTTTTTCTTTAAATTGGTGTCTCCATATTCATGGTATATCTTGCTTTTGCATCCATCATGTTAATGTCTCCAACTTGTTTTGAATTTCTGAAAATGATTGTTTCATTATATCCGTGGTGTGTTCTGTATGTTGTGTCTTGTATTGGTTTTGAGATTTGTAACATAGCTATTCAGATTGATGTTACATTAACTTACTCTTACTCATTTAGAATGTGCATTATATATTCCTTGTGTACCTATTTAGTGAATGAGAATTGAATTGTTTGACAATGAATTAGCCAGGAACAAGATGGTGCTATCAGAAGAGGAAATCACCAGGCTTTTCAGAATTCGAAAAACCGTGTTTCAAATGCTTAGAGACCGAGGCTATTTTGTTGGAGACTTTGAGATTGACATGACAAGGGAGCAATTTAAGCAAAAATATGGTGAAAACATGAAAAGAGAAGACATTGTTATTAATAAATCCAAGAGAAATGATAGCAGTGATCAGGTATTGTCTCAGAAAAGGAGATATGTGATTAGTATTTCTCATGGCTATTAAGTTCAGTTACAAGAACTACAAGTTTCTCGTTAATTCTCTTATTTGTTTACCTCCTCTTATGGTGATTTCGTTTTTCCTGTATAGATATATGTTTTTTTTCCAGAAGAAGCGAAGGTTGGCATCAAGCCGTTGAAAACATATACCACCCGCATGAAATCGGAGAATGTTTTTAGAGCAATATTGGTCGTTCAACAGAATTTGACTCCTTTTGCCCGGGCCTGTATGAGTGAAGCAACTTCCAAATCATTCCACTTAGAAGTTTTCCAGGTGAAAATATTTTATGGAGTATTTTTATGATTTAAGTTTGAGAAAAACCAAACCACCACAAGTTAGAGAAGTAAATTAGATTCTTTTCGTGCACCACTAATTTGAGAAGAATCAAAGGAAACCCTCCTACTCTGGAAACTGGAAATCATCTTATGCTTAGTTTGGTTATTCCTTATTTTGCAAAATGAGAATGAAAATATGGGGGTTTCTTGGATTCTCTGGTTGAAGAACAATTAAAAGGCTAAGGTAGGGCTTGGTTTCTATAATATTCTTGCTCTTTTCTTAGTTCACTTCAAATATACAATTTTCATTCTTTTTCTTATCTTCATCTGTTGATGCAGGAAGCAGAATTGCTAGTTAATATCAAAGATCACGTCCTTGTTCCTGAGCACCAGTTACTTAACAATGAAGAGAAAAAGACACTACTTGAGAGATATACCGTGAAAGAAACACAGGTTAGCATTAATTCTGAAGAAAATATAACATTTGTGAGAACTTATCTTAAGATCTATGTTGGATATTTCTTAAGAAAATTTAACATATATGTGTGTGTGTGTGTTGGAATGTGTTATGCTCATGAATTCCTGATACCAGTTTGATTCCCAAAGTAGACATAAAAATGAAAGAATACACGGGTATACAAAAAAAAAAAAAACTCACAAAAAGGGAGCTCCCTGTACAAAAAGAGCTTAAATAATGTCTATAGAATAGTTACAAAAAGGCTTCAAAATCGAAGCCCACAAAGAAGCATGAAAACGAACGAGGAACCAAAAGTTTGAAGGATCTCTATCCACTTCCCTGGACACTCCATATTTCTTGTCCTGTAAAAGGACTGTAGAGTCATGTGCGTTGACGTTCATGACCTCAACAAAGCCAGTCCCAAGGATGATTTCCCTTCGTCACACATTGACATCTTGGTTACCAATAAAGCAGTGCATGAATCATTAGATATCATCACTATTACTTCACTGATCACAAGGCTATCTAGAGTCGTATGTCAGGCCATACCATCTCGTCTTGACTGTGTCAAAACTGCATCTATTCGTTAGTGTCTATATGTGTTTGCATTTCTGTTGCAATATGAAAGAGATTCTTATATGATTTTGCATATTTGGTGTTAGCTACCACGCATACAAGTGACCGACCCAGTTGCGCGATATTACGGGCTAAAGCGTGGCCAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGAGTATGTTTAAGATTTTGTAATTGAAGATGTGACATATGCATAGACCTTGAAGTTATGTTTTGAAGTCTTAATTAGTGTTATAATGGTTGTGATATCTGAGTTGAGTTGAAGAAGAATTTTGGAATTTCTGTATTAGGGATTTAGATGGGACCTTCATAGTATTAGTTTAGGTGTTGTTGTATACTAGTAGATTTTTATGAACATTGGAAGATTAATTATTTCTATTTAAGTGTACTTTTTTTGTAAATTGTCCCCCTGGAGTGTGGAATTTAGAAATAGCTATCTTTAAA

mRNA sequence

TAACAAAATAAAAAATCCATTAAATCGATTATTTTTAAAAAGTTATTTTAATCAAATTAATTAATAGTCTAAGTTTAAATTCAAATAAAATCAGAGTAAGCCCTAATTTCTCGTTCCACCATCTTCTTCTTCTTCTTCTTCACGCCGGCTTCTCCGCTTCAATTTCAGCAGCTCAGTGACGCCCGTGTTCTACTTAGTGGGTTTTCCTGATTTCTTGTTGTTACTGGTGGTGGTAGTGGTATAGCTCGAAGTTTTATAGTTCCATAATCCTTAAGGCCTTCATCTCCTCAAATGAAAGTTTCCTTTTTTATAAAAAAGAAAATTATATGTAAGTGTCTGGACCAGCTTACATCCAGCCCAACTAATTTCAGACGGGAGAACTTCTGGTGTCGAAGGAAACCTGTCGATATTAAATCCCAAATAGCCAGGAACAAGATGGTGCTATCAGAAGAGGAAATCACCAGGCTTTTCAGAATTCGAAAAACCGTGTTTCAAATGCTTAGAGACCGAGGCTATTTTGTTGGAGACTTTGAGATTGACATGACAAGGGAGCAATTTAAGCAAAAATATGGTGAAAACATGAAAAGAGAAGACATTGTTATTAATAAATCCAAGAGAAATGATAGCAGTGATCAGATATATGTTTTTTTTCCAGAAGAAGCGAAGGTTGGCATCAAGCCGTTGAAAACATATACCACCCGCATGAAATCGGAGAATGTTTTTAGAGCAATATTGGTCGTTCAACAGAATTTGACTCCTTTTGCCCGGGCCTGTATGAGTGAAGCAACTTCCAAATCATTCCACTTAGAAGTTTTCCAGGAAGCAGAATTGCTAGTTAATATCAAAGATCACGTCCTTGTTCCTGAGCACCAGTTACTTAACAATGAAGAGAAAAAGACACTACTTGAGAGATATACCGTGAAAGAAACACAGCTACCACGCATACAAGTGACCGACCCAGTTGCGCGATATTACGGGCTAAAGCGTGGCCAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGAGTATGTTTAAGATTTTGTAATTGAAGATGTGACATATGCATAGACCTTGAAGTTATGTTTTGAAGTCTTAATTAGTGTTATAATGGTTGTGATATCTGAGTTGAGTTGAAGAAGAATTTTGGAATTTCTGTATTAGGGATTTAGATGGGACCTTCATAGTATTAGTTTAGGTGTTGTTGTATACTAGTAGATTTTTATGAACATTGGAAGATTAATTATTTCTATTTAAGTGTACTTTTTTTGTAAATTGTCCCCCTGGAGTGTGGAATTTAGAAATAGCTATCTTTAAA

Coding sequence (CDS)

ATGAAAGTTTCCTTTTTTATAAAAAAGAAAATTATATGTAAGTGTCTGGACCAGCTTACATCCAGCCCAACTAATTTCAGACGGGAGAACTTCTGGTGTCGAAGGAAACCTGTCGATATTAAATCCCAAATAGCCAGGAACAAGATGGTGCTATCAGAAGAGGAAATCACCAGGCTTTTCAGAATTCGAAAAACCGTGTTTCAAATGCTTAGAGACCGAGGCTATTTTGTTGGAGACTTTGAGATTGACATGACAAGGGAGCAATTTAAGCAAAAATATGGTGAAAACATGAAAAGAGAAGACATTGTTATTAATAAATCCAAGAGAAATGATAGCAGTGATCAGATATATGTTTTTTTTCCAGAAGAAGCGAAGGTTGGCATCAAGCCGTTGAAAACATATACCACCCGCATGAAATCGGAGAATGTTTTTAGAGCAATATTGGTCGTTCAACAGAATTTGACTCCTTTTGCCCGGGCCTGTATGAGTGAAGCAACTTCCAAATCATTCCACTTAGAAGTTTTCCAGGAAGCAGAATTGCTAGTTAATATCAAAGATCACGTCCTTGTTCCTGAGCACCAGTTACTTAACAATGAAGAGAAAAAGACACTACTTGAGAGATATACCGTGAAAGAAACACAGCTACCACGCATACAAGTGACCGACCCAGTTGCGCGATATTACGGGCTAAAGCGTGGCCAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGA

Protein sequence

MKVSFFIKKKIICKCLDQLTSSPTNFRRENFWCRRKPVDIKSQIARNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV

Homology

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BLAST of PI0003608 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-88
Identity = 162/206 (78.64%), Postives = 190/206 (92.23%), Query Frame = 0


Query: 50  VLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSKR 109
           +L+EEE+ RL+RI+KT+ QMLRDRGYF+ D E+ MT++QF +K+G+NMKRED+V  K+KR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 110 NDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSKS 169
           ND+SDQ+Y+FFP+EAKVG+K +K YT RMKSENVFRAILVVQQNLTPFAR C+SE +SK 
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK- 120

Query: 170 FHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 229
           FHLEVFQEAE+LVNIK+HVLVPEHQ+L  EEKKTLLERYTVKETQLPRIQVTDP+ARY+G
Sbjct: 121 FHLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFG 180

Query: 230 LKRGQVVKIIRPSETAGRYVTYRYVV 256
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of PI0003608 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.2e-54
Identity = 110/207 (53.14%), Postives = 148/207 (71.50%), Query Frame = 0


Query: 53  EEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGE-----NMKREDIVINKS 112
           EEE  RL++IRKT+ Q+  DRGY V   E+D T E+FK ++G+       +R D+ +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 113 KRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATS 172
             +D +DQ++VFFPEE KVGIK +K Y  RM+ EN+ RA++VVQQ +TP A+  + +   
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 173 KSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARY 232
           K + LE F + ELL+NI +H LVPEH ++  EE   LL RY ++E+QLPRIQ  DPVARY
Sbjct: 124 K-YVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 233 YGLKRGQVVKIIRPSETAGRYVTYRYV 255
           +G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of PI0003608 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.2e-54
Identity = 110/207 (53.14%), Postives = 148/207 (71.50%), Query Frame = 0


Query: 53  EEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGE-----NMKREDIVINKS 112
           EEE  RL++IRKT+ Q+  DRGY V   E+D T E+FK ++G+       +R D+ +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 113 KRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATS 172
             +D +DQ++VFFPEE KVGIK +K Y  RM+ EN+ RA++VVQQ +TP A+  + +   
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 173 KSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARY 232
           K + LE F + ELL+NI +H LVPEH ++  EE   LL RY ++E+QLPRIQ  DPVARY
Sbjct: 124 K-YVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 233 YGLKRGQVVKIIRPSETAGRYVTYRYV 255
           +G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of PI0003608 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.5e-53
Identity = 109/207 (52.66%), Postives = 147/207 (71.01%), Query Frame = 0


Query: 53  EEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGE-----NMKREDIVINKS 112
           EEE  RL++IRKT+ Q+  DRGY V   E+D T E+F+ ++G+       +R D+ +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 113 KRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATS 172
             +D +DQ++VFFPEE KVGIK +K Y  RM+ EN+ RA++VVQQ +TP A+  + +   
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 173 KSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARY 232
           K + LE F + ELL+NI +H LVPEH ++  EE   LL RY ++E QLPRIQ  DPVARY
Sbjct: 124 K-YILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARY 183

Query: 233 YGLKRGQVVKIIRPSETAGRYVTYRYV 255
           +G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of PI0003608 vs. ExPASy Swiss-Prot
Match: Q9N5K2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans OX=6239 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.6e-53
Identity = 114/212 (53.77%), Postives = 148/212 (69.81%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMK-----REDIV 108
           M   E E  RL+RIRKTV QM+ DRGY V   E+D   E FK +YG+        R D+ 
Sbjct: 1   MADDELETYRLWRIRKTVLQMVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLT 60

Query: 109 INKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMS 168
           I  +  +D +DQ++VFFPE+AK+GIK +K    +M+ +N+ RAI+VVQ  +TP A+  + 
Sbjct: 61  ILVAHNDDPADQMFVFFPEDAKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIG 120

Query: 169 EATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDP 228
           +   K + LE F EAEL+VNI +H LVPEH ++  EEK  LL RY +K++QLPRIQ  DP
Sbjct: 121 DMAPK-YMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDP 180

Query: 229 VARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 256
           VARY+GL+RGQVVKIIRPSETAGRY+TYR VV
Sbjct: 181 VARYFGLRRGQVVKIIRPSETAGRYITYRLVV 211

[Close]

BLAST of PI0003608 vs. ExPASy TrEMBL
Match: A0A5D3C9X8 (DNA-directed RNA polymerases II and IV subunit 5A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00310 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.2e-109
Identity = 209/214 (97.66%), Postives = 212/214 (99.07%), Query Frame = 0


Query: 42  SQIARNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED 101
           +++A NKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED
Sbjct: 8   NELAWNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED 67

Query: 102 IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC 161
           IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC
Sbjct: 68  IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC 127

Query: 162 MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVT 221
           MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVT
Sbjct: 128 MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVT 187

Query: 222 DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 256
           DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 188 DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 221

[Close]

BLAST of PI0003608 vs. ExPASy TrEMBL
Match: A0A0A0K8L9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G374590 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.7e-108
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK
Sbjct: 1   MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK
Sbjct: 61  RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
           SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of PI0003608 vs. ExPASy TrEMBL
Match: A0A1S3BS23 (DNA-directed RNA polymerases II and IV subunit 5A OS=Cucumis melo OX=3656 GN=LOC103492579 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 8.4e-108
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK
Sbjct: 1   MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK
Sbjct: 61  RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
           SFHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 SFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of PI0003608 vs. ExPASy TrEMBL
Match: A0A6J1JRV4 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111488357 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 6.0e-98
Identity = 188/207 (90.82%), Postives = 198/207 (95.65%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTV QML+DRGYFVGDFEIDM+REQFK KYGE+MKREDIVINK+K
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEE KVG+K +KTYT RMK+ENVFRAILVVQQNLTPFAR C+SE +SK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
            FHLEVFQEAELLVN+KDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 -FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of PI0003608 vs. ExPASy TrEMBL
Match: A0A6J1CPU1 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX=3673 GN=LOC111013465 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 6.0e-98
Identity = 189/207 (91.30%), Postives = 197/207 (95.17%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTV QML+DR YFVGDFEIDM+REQFK KYGENMKREDIVINK++
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKAR 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEE KVG+K +KTYT RMKSENVFRAILVVQQNLTPFAR C+SE +SK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
            FHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 -FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of PI0003608 vs. NCBI nr
Match: KAA0059617.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis melo var. makuwa] >TYK08150.1 DNA-directed RNA polymerases II and IV subunit 5A [Cucumis melo var. makuwa])

HSP 1 Score: 406.0 bits (1042), Expect = 2.4e-109
Identity = 209/214 (97.66%), Postives = 212/214 (99.07%), Query Frame = 0


Query: 42  SQIARNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED 101
           +++A NKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED
Sbjct: 8   NELAWNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRED 67

Query: 102 IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC 161
           IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC
Sbjct: 68  IVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARAC 127

Query: 162 MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVT 221
           MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVT
Sbjct: 128 MSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVT 187

Query: 222 DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 256
           DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 188 DPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 221

[Close]

BLAST of PI0003608 vs. NCBI nr
Match: XP_004148944.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >XP_011659238.1 DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN44717.1 hypothetical protein Csa_016684 [Cucumis sativus])

HSP 1 Score: 402.1 bits (1032), Expect = 3.5e-108
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK
Sbjct: 1   MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK
Sbjct: 61  RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
           SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of PI0003608 vs. NCBI nr
Match: XP_008451219.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A [Cucumis melo])

HSP 1 Score: 399.8 bits (1026), Expect = 1.7e-107
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK
Sbjct: 1   MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK
Sbjct: 61  RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
           SFHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 SFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of PI0003608 vs. NCBI nr
Match: XP_022143609.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia])

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 189/207 (91.30%), Postives = 197/207 (95.17%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTV QML+DR YFVGDFEIDM+REQFK KYGENMKREDIVINK++
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKAR 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEE KVG+K +KTYT RMKSENVFRAILVVQQNLTPFAR C+SE +SK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
            FHLEVFQEAELLVNIKDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 -FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of PI0003608 vs. NCBI nr
Match: XP_022991826.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023547519.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 188/207 (90.82%), Postives = 198/207 (95.65%), Query Frame = 0


Query: 49  MVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSK 108
           MVLSEEEITRLFRIRKTV QML+DRGYFVGDFEIDM+REQFK KYGE+MKREDIVINK+K
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 109 RNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 168
           RNDSSDQIYVFFPEE KVG+K +KTYT RMK+ENVFRAILVVQQNLTPFAR C+SE +SK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 169 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 228
            FHLEVFQEAELLVN+KDHVLVPEHQLL NEEKKTLLERYTVKETQLPRIQVTDPVARYY
Sbjct: 121 -FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 229 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of PI0003608 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.0e-89
Identity = 162/206 (78.64%), Postives = 190/206 (92.23%), Query Frame = 0


Query: 50  VLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSKR 109
           +L+EEE+ RL+RI+KT+ QMLRDRGYF+ D E+ MT++QF +K+G+NMKRED+V  K+KR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 110 NDSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSKS 169
           ND+SDQ+Y+FFP+EAKVG+K +K YT RMKSENVFRAILVVQQNLTPFAR C+SE +SK 
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK- 120

Query: 170 FHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 229
           FHLEVFQEAE+LVNIK+HVLVPEHQ+L  EEKKTLLERYTVKETQLPRIQVTDP+ARY+G
Sbjct: 121 FHLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFG 180

Query: 230 LKRGQVVKIIRPSETAGRYVTYRYVV 256
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of PI0003608 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 179.1 bits (453), Expect = 4.5e-45
Identity = 97/207 (46.86%), Postives = 146/207 (70.53%), Query Frame = 0


Query: 53  EEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENM---KREDIVINKSKR 112
           ++EITR+F++R+TV QMLRDRGY + + ++++ RE+F Q++ + M    +E + ++ +K 
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 113 NDSSDQIYVFFPEEAKVGIKPL-KTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSK 172
            + +D+IYVF+PE  KVG+  + K    +M+ + V R I+VV   +T  AR  +SE  +K
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSE-LNK 124

Query: 173 SFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYY 232
              +EVF+EAEL+ NI +H LV ++ +L+++ KK LL  YTV++TQLPRI VTDP+ARYY
Sbjct: 125 MLTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYY 184

Query: 233 GLKRGQVVKIIRPSETAGRYVTYRYVV 256
           GLKRGQVVKI R   T+  Y TYR+ V
Sbjct: 185 GLKRGQVVKIRRSDATSLDYYTYRFAV 210

[Close]

BLAST of PI0003608 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 136.7 bits (343), Expect = 2.6e-32
Identity = 79/215 (36.74%), Postives = 130/215 (60.47%), Query Frame = 0


Query: 41  KSQIARNKMVLSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKRE 100
           K  +  + +  S  E  + +  R+T  +MLRDRGY V D +I+++ +QF+  YGE+   +
Sbjct: 6   KEIVVGHSISKSSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVD 65

Query: 101 DIVINKSKRNDSSDQIYVFFPEEAKVGIKPLKTYTTRMKS-ENVFRAILVVQQNLTPFAR 160
            + I+   R DSS +I V F     V +  ++     + S EN+   ILV+Q ++T    
Sbjct: 66  LLRISAKHRFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHIT---N 125

Query: 161 ACMSEATSKSFHLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQ 220
             +      SF +E+F+  +LLVN+  HVL P+HQ+LN++EK++LL++++++E QLPR+ 
Sbjct: 126 QALKAVELFSFKVELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLS 185

Query: 221 VTDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYV 255
             DP+ RYYGL+ GQV+K+    E +  +VTYR V
Sbjct: 186 SKDPIVRYYGLETGQVMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of PI0003608 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 132.5 bits (332), Expect = 4.8e-31
Identity = 75/204 (36.76%), Postives = 119/204 (58.33%), Query Frame = 0


Query: 51  LSEEEITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSKRN 110
           LS EE  R +  R+   QMLRDRGY V D +I+++   F+  YGE    + + I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 111 DSSDQIYVFFPEEAKVGIKPLKTYTTRMKSENVFRAILVVQQNLTPFARACMSEATSKSF 170
           DS+ ++ + F   + V +  +++    + S+     +++V QN        +      SF
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQN--HVTNQALKAIELFSF 139

Query: 171 HLEVFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 230
            +E+FQ  +LLVNI  H L P+HQ+LN+EEK TLL++++++E QLPRI   D + RYYGL
Sbjct: 140 KVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGL 199

Query: 231 KRGQVVKIIRPSETAGRYVTYRYV 255
           ++GQVVK+    E    +V +R V
Sbjct: 200 EKGQVVKVNYRGELTESHVAFRCV 221

[Close]

BLAST of PI0003608 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-27
Identity = 72/202 (35.64%), Postives = 114/202 (56.44%), Query Frame = 0


Query: 55  EITRLFRIRKTVFQMLRDRGYFVGDFEIDMTREQFKQKYGENMKREDIVINKSKRNDSSD 114
           E  R +  R T F+MLRDRGY V + E+ +T  +F+  +GE  + E + I    R+D   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 115 QIYVFFPEEAKVGIKPLKTYTTRMKSE-NVFRAILVVQQNLTPFARACMSEATSKSFHLE 174
           +I V F     + +K ++    ++ +   +   ILV+Q  +  FA+  +   T+  F +E
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKAL---TTFPFTVE 154

Query: 175 VFQEAELLVNIKDHVLVPEHQLLNNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRG 234
            F   +LLVNI  H+  P+ ++LN EEK+ LL ++ +++ QLP +Q  D   RYYGLK+ 
Sbjct: 155 TFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKK 214

Query: 235 QVVKIIRPSETAGRYVTYRYVV 256
           QVVKI    E  G +VTYR ++
Sbjct: 215 QVVKITYSKEPVGDFVTYRCII 233

The following BLAST results are available for this feature:

BLAST of PI0003608 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	1.4e-88	78.64	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	5.2e-54	53.14	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	5.2e-54	53.14	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q5R587	1.5e-53	52.66	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q9N5K2	2.6e-53	53.77	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis ele...	[more]

BLAST of PI0003608 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C9X8	1.2e-109	97.66	DNA-directed RNA polymerases II and IV subunit 5A OS=Cucumis melo var. makuwa OX...	[more]
A0A0A0K8L9	1.7e-108	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G374590 PE=3 SV=1	[more]
A0A1S3BS23	8.4e-108	99.52	DNA-directed RNA polymerases II and IV subunit 5A OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1JRV4	6.0e-98	90.82	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1CPU1	6.0e-98	91.30	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX...	[more]

BLAST of PI0003608 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0059617.1	2.4e-109	97.66	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis melo var. makuwa] >TY...	[more]
XP_004148944.1	3.5e-108	100.00	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >XP_01165923...	[more]
XP_008451219.1	1.7e-107	99.52	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A [Cucumis melo]	[more]
XP_022143609.1	1.2e-97	91.30	DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia]	[more]
XP_022991826.1	1.2e-97	90.82	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_02...	[more]

BLAST of PI0003608 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	1.0e-89	78.64	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	4.5e-45	46.86	RNA polymerase II fifth largest subunit, C	[more]
AT2G41340.1	2.6e-32	36.74	RNA polymerase II fifth largest subunit, D	[more]
AT3G57080.1	4.8e-31	36.76	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT3G54490.1	1.1e-27	35.64	RNA polymerase II fifth largest subunit, E	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 182..254 e-value: 1.6E-34 score: 117.6
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 180..255 e-value: 1.7E-34 score: 119.3
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 180..255
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 48..255 e-value: 2.2E-88 score: 293.1
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 53..138 e-value: 1.5E-31 score: 108.9
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 51..179 e-value: 1.2E-45 score: 156.9
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 53..183
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 49..255
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 49..255
IPR020608	RNA polymerase, subunit H/Rpb5, conserved site	PROSITE	PS01110	RNA_POL_H_23KD	coord: 187..200
IPR020609	Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit	HAMAP	MF_00025	RNApol_RpoH_RPB5	coord: 182..255 score: 22.768368

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003608.3	PI0003608.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity