PI0001973 (gene) Melon (PI 482460) v1

BLAST of PI0001973 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 5.6e-158
Identity = 281/326 (86.20%), Postives = 309/326 (94.79%), Query Frame = 0

Query: 51  IRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 110
           IRAS+AVEQQ ++ K AL+RIGTRGSPLALAQAHETRDKLMASH ELAE+GAIQIV+IKT
Sbjct: 44  IRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETRDKLMASHTELAEEGAIQIVIIKT 103

Query: 111 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 170
           TGDKILSQPLADIGGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLPE+TILPCNLPRED
Sbjct: 104 TGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPEETILPCNLPRED 163

Query: 171 VRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEG 230
           VRDAFISLSA SLA+LPAGS+IGTASLRRKSQ+L+RYPSL V +NFRGNVQTRLRKL+EG
Sbjct: 164 VRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRYPSLTVQDNFRGNVQTRLRKLSEG 223

Query: 231 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHE 290
           VV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQGAIGIACRS+DD MA YLASLNHE
Sbjct: 224 VVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQGAIGIACRSNDDKMAEYLASLNHE 283

Query: 291 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYA 350
           ETRLA+ CERAFL TLDGSCRTPIAGYASRD+DGNC+F+GLVASPDGTRVLETSR G Y 
Sbjct: 284 ETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCLFRGLVASPDGTRVLETSRIGSYT 343

Query: 351 IDDMIAMGKDAGQELLSQAGPGFFDS 377
            +DM+ +GKDAG+ELLS+AGPGFF+S
Sbjct: 344 YEDMMKIGKDAGEELLSRAGPGFFNS 369

BLAST of PI0001973 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 4.0e-156
Identity = 278/357 (77.87%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 18  CNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQVEKTKVALVRIGTRGSP 77
           C++GS+S +GFS   +  PA     +K    G ++A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 78  LALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDAL 137
           LALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 138 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASL 197
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGS++GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 198 RRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSI 257
           RRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 258 DEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 317
           DEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 318 ASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKDAGQELLSQAGPGFF 375
           AS+DE+GNCIF+GLVASPDGT+VLETSR+GPY  +DM+ MGKDAGQELLS+AGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of PI0001973 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 4.1e-140
Identity = 251/326 (76.99%), Postives = 286/326 (87.73%), Query Frame = 0

Query: 51  IRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 110
           +RA+VAV+ + +  KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKT
Sbjct: 33  VRAAVAVQAEAQ-AKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 111 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 170
           TGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 171 VRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEG 230
           VRDAFI L+A SLAELPAGS++G+ASLRR+SQ+L +YPSL V+ NFRGNVQTRLRKL EG
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 231 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHE 290
            V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M NYL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 291 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYA 350
           +TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR GPY 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 351 IDDMIAMGKDAGQELLSQAGPGFFDS 377
            D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

BLAST of PI0001973 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.9e-84
Identity = 172/307 (56.03%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 69  VRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 128
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 129 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPA 188
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 189 GSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 248
           G+++G++SLRR++QL  R P L ++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 249 ENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDG 308
                 +  DEMLPAVAQG IG+  R+DD   A+ LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 309 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKDAGQELLSQ 368
           SC+TPIAG A    D     +G +  PDG+ V+   R GP A  D  AMG D  +EL  +
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 369 AGPGFFD 376
           A   FFD
Sbjct: 311 APADFFD 311

BLAST of PI0001973 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.6e-83
Identity = 173/316 (54.75%), Postives = 215/316 (68.04%), Query Frame = 0

Query: 59  QQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 118
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 119 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 178
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 179 SAGSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLA 238
            AGSL +LPAG+ +GTASLRR++ +L   P L V  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 239 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVC 298
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 299 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMG 358
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R+G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 359 KDAGQELLSQAGPGFF 375
            DA  EL  +AG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

BLAST of PI0001973 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.5e-195
Identity = 354/376 (94.15%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPPAFS G KKFHG GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.6e-195
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPPAFS G K+FHGVGLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.5e-193
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           M  LSSPSVS+SPM RPCN+ SLSL GFSSLSLKPPAF+N  KKFHG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS++GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+G YAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 351/376 (93.35%), Postives = 360/376 (95.74%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           M  LSSPSVSHSPM  PCN+ SLSL GFSSLSLKPPAF+NG KK HG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
             KTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS++GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+G YAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.1e-192
Identity = 347/376 (92.29%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGV SSP ++HSPM RPCNVGSLS LGFSSLSLKPPAF NG KKF G+GL+RASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSR+G YAIDDM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 710.7 bits (1833), Expect = 6.8e-201
Identity = 367/376 (97.61%), Postives = 371/376 (98.67%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGVLSSPSVSHSPMPRP NVGSLSLLGFSSLSLKPPAFSNGAKKFHG GLIRASVAVEQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSIIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACR+DDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 709.1 bits (1829), Expect = 2.0e-200
Identity = 366/376 (97.34%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPP FSNGAKKFHGVGLIRA VA EQQ
Sbjct: 57  MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
           VEKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGSIIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER
Sbjct: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 356

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAI+DMIAMGKD
Sbjct: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416

Query: 361 AGQELLSQAGPGFFDS 377
           AGQELLS+AGPGFFDS
Sbjct: 417 AGQELLSRAGPGFFDS 431

BLAST of PI0001973 vs. NCBI nr
Match: XP_004149732.1 (porphobilinogen deaminase, chloroplastic [Cucumis sativus])

HSP 1 Score: 709.1 bits (1829), Expect = 2.0e-200
Identity = 366/376 (97.34%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPP FSNGAKKFHGVGLIRA VA EQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
           VEKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GS AELPAGSIIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AGQELLS+AGPGFFDS
Sbjct: 361 AGQELLSRAGPGFFDS 375

BLAST of PI0001973 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.5e-195
Identity = 355/376 (94.41%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPPAFS GAKKFHG GLIRASVAVEQQ
Sbjct: 18  MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQ 77

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 78  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 137

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 138 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 197

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 198 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 257

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 258 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 317

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAIDDMIAMGKD
Sbjct: 318 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 377

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 378 AGKELLSRAGPGFFDS 393

BLAST of PI0001973 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 690.6 bits (1781), Expect = 7.3e-195
Identity = 354/376 (94.15%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQ 60
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPPAFS G KKFHG GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  VEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR+GPYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGQELLSQAGPGFFDS 377
           AG+ELLS+AGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of PI0001973 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 552.4 bits (1422), Expect = 2.9e-157
Identity = 278/357 (77.87%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 18  CNVGSLSLLGFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQVEKTKVALVRIGTRGSP 77
           C++GS+S +GFS   +  PA     +K    G ++A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 78  LALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDAL 137
           LALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 138 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASL 197
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGS++GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 198 RRKSQLLNRYPSLIVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSI 257
           RRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 258 DEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 317
           DEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 318 ASRDEDGNCIFKGLVASPDGTRVLETSRQGPYAIDDMIAMGKDAGQELLSQAGPGFF 375
           AS+DE+GNCIF+GLVASPDGT+VLETSR+GPY  +DM+ MGKDAGQELLS+AGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380