PI0000381 (gene) Melon (PI 482460) v1

BLAST of PI0000381 vs. ExPASy Swiss-Prot
Match: Q8GYD9 (Probable RNA helicase SDE3 OS=Arabidopsis thaliana OX=3702 GN=SDE3 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-49
Identity = 140/484 (28.93%), Postives = 256/484 (52.89%), Query Frame = 0

Query: 192 SKTSSLPSTPTTSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIK 251
           S TS+ P + +   P+     +  ++ +  SK   + +     N LPLY +PK++ ++I+
Sbjct: 173 SLTSNRPYSRSRRAPKKDFAVDDYVKGSRPSKVVERSF----RNRLPLYEIPKEIREMIE 232

Query: 252 NDIVPQVLRKPLSPLTYKSYFAALLYAEDVYYKK-WSNYKLKSVSLELQQITIHKGPNKK 311
           N   P  L + L+   Y +Y+  LL  E++  ++    Y +++VS++ + I         
Sbjct: 233 NKEFPDDLNEGLTARNYANYYKTLLIMEELQLEEDMRAYDMENVSMKRRGI--------- 292

Query: 312 TKLNSHEVIKTFVAFEIDSVPERRPFLLSRDLVHARLC--EKTLEPFEGFVYRIAKSNCP 371
                      +++ E+  + ERRP L+  D +  R    + T   ++GFV+R+      
Sbjct: 293 -----------YLSLEVPGLAERRPSLVHGDFIFVRHAYDDGTDHAYQGFVHRV------ 352

Query: 372 LGKKNVLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKAT 431
             + + + + F  EF+ RH   + Y++ FT+NR+  +R +QA+ +A++ L  NFLFP   
Sbjct: 353 --EADEVHMKFASEFHQRHTAGSVYNVRFTYNRINTRRLYQAV-DAAEMLDPNFLFPSLH 412

Query: 432 SR----KVQPYFQVKHSSHQQLDHHQKNAIHQILHLHGSPPYLIKGPP------YVEGAV 491
           S     K +P+  +  +    L+  Q  +I  +L   G+PPY+I GPP       +  A+
Sbjct: 413 SGKRMIKTKPFVPISPA----LNAEQICSIEMVLGCKGAPPYVIHGPPGTGKTMTLVEAI 472

Query: 492 FQLYSTSPNCKILICAPRNATCDKL---MISLKKV-IPDSKMFRATAAFRERDEVPDDIL 551
            QLY+T  N ++L+CAP N+  D +   ++ L+ V I D+++FR  AA R  +E+  +I+
Sbjct: 473 VQLYTTQRNARVLVCAPSNSAADHILEKLLCLEGVRIKDNEIFRLNAATRSYEEIKPEII 532

Query: 552 PLCDYNEFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEP 611
             C ++E    F CP L  L +YK+++ST++S+  L+A+G+  GHF+HI L +A +A EP
Sbjct: 533 RFCFFDEL--IFKCPPLKALTRYKLVVSTYMSASLLNAEGVNRGHFTHILLDEAGQASEP 592

Query: 612 EVLVPLTKFVIDTTNVIVTGETGKQPYWVRSMIARKHGLRTSYFERLGEMMPY-RSNPSF 658
           E ++ ++   +  T V++ G+  +    + S  A   GL  SY ERL E   Y   + ++
Sbjct: 593 ENMIAVSNLCLTETVVVLAGDPRQLGPVIYSRDAESLGLGKSYLERLFECDYYCEGDENY 617

BLAST of PI0000381 vs. ExPASy Swiss-Prot
Match: Q5ZKD7 (Putative helicase MOV-10 OS=Gallus gallus OX=9031 GN=MOV10 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.2e-32
Identity = 108/406 (26.60%), Postives = 192/406 (47.29%), Query Frame = 0

Query: 258 VLRKPLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE 317
           +L  PL P  YK  F  LL+ E++            + +++++  + + P  +       
Sbjct: 323 LLEAPLQPENYKQKFELLLHLEEI-----------QLEVDIRRYDLQEVPMVQN------ 382

Query: 318 VIKTFVAFEIDSVPERRPFLLSRDLVHARL-CEKTLEP---FEGFVYRIAKSNCPLGKKN 377
             +  +   +  V E RP +L  D + A L  E+   P   ++G+V+ +      LG   
Sbjct: 383 --RALLLLNVPGVAENRPSVLRGDHLFASLSSERDSSPRVLYKGYVHGVELERVQLGFSP 442

Query: 378 VLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRK-- 437
            L+  F  +         K+D++FTFNR+ L+  H+A   A    + + LFP A+ ++  
Sbjct: 443 KLMKKFVNDL--------KFDVTFTFNRLPLQVQHRAAAMAMQKGLDSVLFPSASCQRSL 502

Query: 438 VQPYFQVKHSSHQ-QLDHHQKNAI-HQILHLHGSPPYLIKGPP------YVEGAVFQLYS 497
               FQ +    + + +  Q  A+ H +  +    PYLI GPP       +  A+ Q++S
Sbjct: 503 FTGIFQPRWFDRKVEANEEQCQAVKHIVTGMSRPAPYLIFGPPGTGKTVTLVEAIKQVWS 562

Query: 498 TSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNEFQ 557
              + +IL CAP N+  D L   L   I    ++R  A+     +VP D+ P C++++ +
Sbjct: 563 CFKDARILACAPSNSAADLLCQRLLTNIAPRYIYRIMASSANYKDVPADVRPCCNWDDSE 622

Query: 558 ECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVP---- 617
           +C+  P    L  Y+I+++T +++ RL +     G+FSH+F+ +   A+EPE +V     
Sbjct: 623 KCYVYPSKKLLKPYRILITTLVTAGRLVSANFPPGYFSHVFIDECGHAVEPESVVAIAGL 682

Query: 618 LTKFVIDTT----NVIVTGETGKQPYWVRSMIARKHGLRTSYFERL 642
           LT    DT      +++ G+  +     RS +A +HGL TS  ERL
Sbjct: 683 LTTMDPDTNPNGGQLVLAGDPQQLGPVPRSPLAAQHGLGTSLLERL 701

BLAST of PI0000381 vs. ExPASy Swiss-Prot
Match: Q1LXK5 (Putative helicase mov-10-B.2 OS=Danio rerio OX=7955 GN=mov10b.2 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.1e-27
Identity = 114/443 (25.73%), Postives = 200/443 (45.15%), Query Frame = 0

Query: 235 NSLPL--YLVPKD---VEDLIKNDIVPQVLRKPL--------SPLTYKSY---FAALLYA 294
           N LPL  Y+ P D   + +L+K D   ++ R  L        SPLT+ +Y   F  LLY 
Sbjct: 321 NVLPLGKYIPPPDTTILAELLKEDCSSRIFRGKLKDLQSLLQSPLTFDNYAERFDLLLYL 380

Query: 295 ED----VYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHEVIKTFVAFEIDSVPERR 354
           E+    V  K+++             +T+H+  +K+            +   +  V E R
Sbjct: 381 EECQMHVDIKRYNK----------DSVTLHRDRDKR-----------LMGLNLPGVSENR 440

Query: 355 PFLLSRDLVHARLCEK----TLEPFEGFVYRIAKSNCPLGKKNVLLVDFGEEFYARHCGT 414
           P +L  D +     E+     +  ++G+V+R+      LG     L D        +   
Sbjct: 441 PSVLRGDHLLLTKSEEVTFSNVTKYKGYVHRVELDQVKLGLSKRFLKDV-------YIDK 500

Query: 415 NKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATS--RKVQPYFQVKHSSHQQLDH 474
            K+ + FT NR+ ++  H+A+  A    +++ LFP A+     V P          + + 
Sbjct: 501 MKFRVEFTVNRIPVRLQHRAVHMAVQHNLKDVLFPMASRSLNPVSPPALRLFDRKLEYNF 560

Query: 475 HQKNAIHQILHLHGSP-PYLIKGPP------YVEGAVFQLYSTSPNCKILICAPRNATCD 534
            Q +A+  I+     P PYL+ GPP       +  A+ Q+    P+  IL CAP N+  D
Sbjct: 561 QQYSAVCNIVAGTSKPAPYLVFGPPGTGKTVTIVEAIKQVEKNIPDAYILACAPSNSAAD 620

Query: 535 KLMISL--KKVIPDSKMFRATAAFRERDEVPDDILPLCDYNEFQECFTCPELDELDKYKI 594
           +L   L   + +   K++R  A+ R + ++P  +   C+ +E    F C E  +L  YKI
Sbjct: 621 QLCEKLITSEHVDAHKIYRLYASSRNQKDIPKILKDNCNVDEEMIDFPCKE--DLMSYKI 680

Query: 595 ILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLTKFV-IDTTNVIVTGETGK 642
           ++ T +++ RL   G +  HF+H F+ +A  A+E E ++ +   +  +   +++ G+  +
Sbjct: 681 LICTLVTAGRLVTGGFSEDHFTHNFVDEAGHAVESETIISVAGLLNAEKGQLVLAGDPKQ 733

BLAST of PI0000381 vs. ExPASy Swiss-Prot
Match: Q1LXK4 (Putative helicase mov-10-B.1 OS=Danio rerio OX=7955 GN=mov10b.1 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 6.2e-27
Identity = 95/350 (27.14%), Postives = 161/350 (46.00%), Query Frame = 0

Query: 308 NKKTKLNSHEVIKTFVAFEIDSVPERRPFLLSRDLV----HARLCEKTLEPFEGFVYRIA 367
           NKK      +  K  +  E+  V E RP +L  D +       L    +  ++G+V+R+ 
Sbjct: 389 NKKDVSMVRDRDKKLLVLELPGVSENRPSVLRGDHLLLTKSEELQNSNVTKYKGYVHRVE 448

Query: 368 KSNCPLGKKNVLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFL 427
                LG        F +    R     K+ + FT NR+ L+  H+A+       +++ L
Sbjct: 449 LDQVKLG--------FSKRLLERFIDNMKFSVEFTINRLPLRLQHRAVHMVVQHHLKDVL 508

Query: 428 FPKATSR--KVQPYFQVKHSSHQQLDHHQKNAIHQILHLHGSP-PYLIKGPP------YV 487
           FP A+ R   V P          + +  QK A+  I+     P PYL+ GPP       +
Sbjct: 509 FPVASRRLNPVSPSALRLFDQKLEKNPEQKTAVCNIVAGTSKPAPYLVFGPPGTGKTVTI 568

Query: 488 EGAVFQLYSTSPNCKILICAPRNATCDKL--MISLKKVIPDSKMFRATAAFRERDEVPDD 547
             A+ Q+   +   +IL CAP N+  D+L   +   + +    ++R  A+ R   E+P  
Sbjct: 569 VEAIKQVEKNTGGARILACAPSNSAADQLGEKLITSQHVDARNIYRIYASSRNPKEIPKV 628

Query: 548 ILPLCDYNEFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAI 607
           +    +       F C E  +L  YKI++ T +++ RL + G   GHFSHIF+ +A  A+
Sbjct: 629 LENNSNVEGENIIFPCKE--DLMPYKIVVCTLVTAGRLVSGGFPVGHFSHIFVDEAGHAV 688

Query: 608 EPEVLVPLTKFV-IDTTNVIVTGETGKQPYWVRSMIARKHGLRTSYFERL 642
           EPE+++ +   +  +T  +++ G+  +    +RS  A K+GL  S  ERL
Sbjct: 689 EPEIVISVAGLLNAETGQLVLAGDPKQLGPILRSPFAIKYGLGLSLLERL 728

BLAST of PI0000381 vs. ExPASy Swiss-Prot
Match: Q9HCE1 (Helicase MOV-10 OS=Homo sapiens OX=9606 GN=MOV10 PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 3.1e-26
Identity = 108/416 (25.96%), Postives = 190/416 (45.67%), Query Frame = 0

Query: 285 KWSNYKLK----------SVSLELQQITIHKGPNKKTKLNSHEVIKTFVAFEIDSVPERR 344
           KW NY++K           +  +++   +   P     ++ +  + T    E+  V E R
Sbjct: 332 KWRNYEVKLRLLLHLEELQMEHDIRHYDLESVPMTWDPVDQNPRLLT---LEVPGVTESR 391

Query: 345 PFLLSRDLVHARLCEKTLE----PFEGFVYRIAKSNCPLGKKNVLLVDFGEEFYARHCGT 404
           P +L  D + A L  +T +     ++GFV+++      L         F     +R    
Sbjct: 392 PSVLRGDHLFALLSSETHQEDPITYKGFVHKVELDRVKL--------SFSMSLLSRFVDG 451

Query: 405 NKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKA--------TSRKVQPYFQVKHSS 464
             + ++FTFNR  L+  H+A+E     L+   LFP A        +  K++ Y +   S+
Sbjct: 452 LTFKVNFTFNRQPLRVQHRALELTGRWLLWPMLFPVAPRDVPLLPSDVKLKLYDRSLESN 511

Query: 465 HQQLDHHQKNAIHQILHLHGSPPYLIKGPP------YVEGAVFQLYSTSPNCKILICAPR 524
            +QL   +    H +       PY+I GPP       +  A+ Q+    P   IL CAP 
Sbjct: 512 PEQLQAMR----HIVTGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPS 571

Query: 525 NATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNEFQECFTCPELDELDK 584
           N+  D L   L+  +P S ++R  A  R+   VP+DI P C+++  +  +  P   +L +
Sbjct: 572 NSGADLLCQRLRVHLP-SSIYRLLAPSRDIRMVPEDIKPCCNWDAKKGEYVFPAKKKLQE 631

Query: 585 YKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLTKFV-IDTT-----NV 644
           Y+++++T I++ RL +      HF+HIF+ +A   +EPE LV +   + +  T      +
Sbjct: 632 YRVLITTLITAGRLVSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMEVKETGDPGGQL 691

Query: 645 IVTGETGKQPYWVRSMIARKHGLRTSYFERLGEMMPYRS---------NPSFISEL 658
           ++ G+  +    +RS + +KHGL  S  ERL   + Y S         +P FI++L
Sbjct: 692 VLAGDPRQLGPVLRSPLTQKHGLGYSLLERL---LTYNSLYKKGPDGYDPQFITKL 728

BLAST of PI0000381 vs. ExPASy TrEMBL
Match: A0A6J1FE20 (probable RNA helicase SDE3 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111444680 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 3.3e-249
Identity = 489/717 (68.20%), Postives = 541/717 (75.45%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   ++V DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSDVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L Y HYPNY K KESTLE+S+  S+NAKTS+  LLQHSSK QSSPVFPKPSS        
Sbjct: 66  LYYYHYPNYSKAKESTLEKSSTASYNAKTSN--LLQHSSKPQSSPVFPKPSSHAAAPDCG 125

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSI-NASSSSSSF 180
                         ++L + ST                  SS+ TPSGSI ++SSSSSSF
Sbjct: 126 KSNSSSFRAHSVSSSTLEKSSTASYNAKTSSPLQHYSKPQSSHSTPSGSISSSSSSSSSF 185

Query: 181 RTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPT 240
             +SL  +  + Q  +YL SPSP  S LT+SSVNQLVSS+KL T TPKPS    LPS  T
Sbjct: 186 GAHSLSSSLLRPQEISYLRSPSPVSSDLTRSSVNQLVSSSKLPTPTPKPS--YFLPSPST 245

Query: 241 TSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRKP 300
           TS PEPP    PTL   SSSK N QEYILD+++   LYL PKD+EDLIKNDIVP VLRKP
Sbjct: 246 TSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLSTLYLAPKDIEDLIKNDIVPHVLRKP 305

Query: 301 LSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIKT 360
           LSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE V KT
Sbjct: 306 LSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVTKT 365

Query: 361 FVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFGE 420
           FVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYR++KSN  L   N+LLV+FGE
Sbjct: 366 FVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRVSKSNYQLRMNNILLVNFGE 425

Query: 421 EFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKHS 480
           +F++RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  S
Sbjct: 426 DFHSRHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVNPYIEVTRS 485

Query: 481 SHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQL 540
             Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ+
Sbjct: 486 GQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITRTGEVVVGAVFQI 545

Query: 541 YSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNE 600
           YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN 
Sbjct: 546 YSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYNR 605

Query: 601 FQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLT 658
            QECF CP LDEL KYKII STF+SSFRL  KG A GHFSHIFLLDAS AIEPEVLVPLT
Sbjct: 606 DQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAPGHFSHIFLLDASAAIEPEVLVPLT 665

BLAST of PI0000381 vs. ExPASy TrEMBL
Match: A0A6J1FIW9 (probable RNA helicase SDE3 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111444680 PE=4 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 5.0e-245
Identity = 476/681 (69.90%), Postives = 524/681 (76.95%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   ++V DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSDVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTVTSLNR 120
           L Y HYPNY K KESTLE+S+  S+NAKTSSP  LQH SK Q                  
Sbjct: 66  LYYYHYPNYSKAKESTLEKSSTASYNAKTSSP--LQHYSKPQ------------------ 125

Query: 121 KSTLSSYLTPSGSI-NASSSSSSFRTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQL 180
               SS+ TPSGSI ++SSSSSSF  +SL  +  + Q  +YL SPSP  S LT+SSVNQL
Sbjct: 126 ----SSHSTPSGSISSSSSSSSSFGAHSLSSSLLRPQEISYLRSPSPVSSDLTRSSVNQL 185

Query: 181 VSSTKLTTSTPKPSKTSSLPSTPTTSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLP 240
           VSS+KL T TPKPS    LPS  TTS PEPP    PTL   SSSK N QEYILD+++   
Sbjct: 186 VSSSKLPTPTPKPS--YFLPSPSTTSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLST 245

Query: 241 LYLVPKDVEDLIKNDIVPQVLRKPLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLEL 300
           LYL PKD+EDLIKNDIVP VLRKPLSP TYKSYFAALLYAED YYKKWS+YKL++VSLEL
Sbjct: 246 LYLAPKDIEDLIKNDIVPHVLRKPLSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLEL 305

Query: 301 QQITIHKGPNKKTKLNSHE-VIKTFVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEG 360
           QQITIHKGPNKKTK N HE V KTFVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G
Sbjct: 306 QQITIHKGPNKKTKFNGHEKVTKTFVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQG 365

Query: 361 FVYRIAKSNCPLGKKNVLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDS 420
            VYR++KSN  L   N+LLV+FGE+F++RH  T+KYDISFTFNRVCLKRAHQAIEEASDS
Sbjct: 366 LVYRVSKSNYQLRMNNILLVNFGEDFHSRHRKTDKYDISFTFNRVCLKRAHQAIEEASDS 425

Query: 421 LIQNFLFPKATSRKVQPYFQVKHSSHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE-- 480
           L+Q+FLFPK+TSRKV PY +V  S  Q LD  QKNAI QIL L GSPPYLI+G P V   
Sbjct: 426 LLQSFLFPKSTSRKVNPYIEVTRSGQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSY 485

Query: 481 ------------------GAVFQLYSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMF 540
                             GAVFQ+YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MF
Sbjct: 486 DWAENQTRKITRTGEVVVGAVFQIYSTSPNCKILICAPRNTTCDELMISLKKVIPESNMF 545

Query: 541 RATAAFRERDEVPDDILPLCDYNEFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAA 600
           RA AAFRERDEVPDDILPLCDYN  QECF CP LDEL KYKII STF+SSFRL  KG A 
Sbjct: 546 RAIAAFRERDEVPDDILPLCDYNRDQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAP 605

Query: 601 GHFSHIFLLDASEAIEPEVLVPLTKFVIDTTNVIVTGETGKQPYWVRSMIARKHGLRTSY 658
           GHFSHIFLLDAS AIEPEVLVPLTKF ID TNVIVTG+ G QPYWVRS IAR+HGL+ SY
Sbjct: 606 GHFSHIFLLDASAAIEPEVLVPLTKFAIDATNVIVTGQRGNQPYWVRSQIARRHGLKISY 658

BLAST of PI0000381 vs. ExPASy TrEMBL
Match: A0A6J1JVI0 (probable RNA helicase SDE3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489245 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.4e-244
Identity = 485/718 (67.55%), Postives = 533/718 (74.23%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFLDIL CF CCCDDP+ DNN+ EIYTANSS R   +NV DSSS QK Q DTS +TPL
Sbjct: 1   MSVFLDILRCFCCCCDDPDHDNNYREIYTANSSERFRVSNVSDSSSYQKIQPDTSAVTPL 60

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L YDHYPNY K KESTLE+S+  S+NAKTS+  L QHSSK QSSPVF KPSS        
Sbjct: 61  LYYDHYPNYSKAKESTLEKSSTASYNAKTSN--LYQHSSKPQSSPVFSKPSSHAAAPNCG 120

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSINASSSSSSFR 180
                         ++L + ST                  SS+ TPSGSI+ SSSSSSFR
Sbjct: 121 KSSSSSFGAHSLSSSTLEKSSTASYNAKTSSLLQHSSKPQSSHPTPSGSISPSSSSSSFR 180

Query: 181 TNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPTT 240
            +SL  +  + Q T+YL SPSP  S L +SSVNQLVSS+KL T TPKPS   SLPS  TT
Sbjct: 181 AHSLSSSLLRPQETSYLRSPSPVSSDLPRSSVNQLVSSSKLPTPTPKPS--YSLPSPSTT 240

Query: 241 SLPEPP--PTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRK 300
           S PEPP   T +PT                     L LYL PKDVE+LIKNDI P VLRK
Sbjct: 241 SFPEPPREQTLHPT---------------------LTLYLAPKDVENLIKNDIEPHVLRK 300

Query: 301 PLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIK 360
           PLSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE VIK
Sbjct: 301 PLSPSTYKSYFAALLYAEDYYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVIK 360

Query: 361 TFVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFG 420
           TFVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYRI+ SN  L  KN+LLV+FG
Sbjct: 361 TFVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRISTSNYQLSMKNILLVNFG 420

Query: 421 EEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKH 480
           E+F+ RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  
Sbjct: 421 EDFHFRHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVDPYIKVTR 480

Query: 481 SSHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQ 540
           S  Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ
Sbjct: 481 SGQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITKTGEVVVGAVFQ 540

Query: 541 LYSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYN 600
           +YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN
Sbjct: 541 IYSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYN 600

Query: 601 EFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPL 658
             QECFTCP LD+L KYKII STF+SSFRLH KGLAAGHFSHIFLLDAS AIEPEVLVPL
Sbjct: 601 RDQECFTCPSLDKLHKYKIIFSTFMSSFRLHPKGLAAGHFSHIFLLDASAAIEPEVLVPL 660

BLAST of PI0000381 vs. ExPASy TrEMBL
Match: A0A6J1JVH4 (probable RNA helicase SDE3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489245 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.4e-244
Identity = 485/718 (67.55%), Postives = 533/718 (74.23%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFLDIL CF CCCDDP+ DNN+ EIYTANSS R   +NV DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLDILRCFCCCCDDPDHDNNYREIYTANSSERFRVSNVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L YDHYPNY K KESTLE+S+  S+NAKTS+  L QHSSK QSSPVF KPSS        
Sbjct: 66  LYYDHYPNYSKAKESTLEKSSTASYNAKTSN--LYQHSSKPQSSPVFSKPSSHAAAPNCG 125

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSINASSSSSSFR 180
                         ++L + ST                  SS+ TPSGSI+ SSSSSSFR
Sbjct: 126 KSSSSSFGAHSLSSSTLEKSSTASYNAKTSSLLQHSSKPQSSHPTPSGSISPSSSSSSFR 185

Query: 181 TNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPTT 240
            +SL  +  + Q T+YL SPSP  S L +SSVNQLVSS+KL T TPKPS   SLPS  TT
Sbjct: 186 AHSLSSSLLRPQETSYLRSPSPVSSDLPRSSVNQLVSSSKLPTPTPKPS--YSLPSPSTT 245

Query: 241 SLPEPP--PTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRK 300
           S PEPP   T +PT                     L LYL PKDVE+LIKNDI P VLRK
Sbjct: 246 SFPEPPREQTLHPT---------------------LTLYLAPKDVENLIKNDIEPHVLRK 305

Query: 301 PLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIK 360
           PLSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE VIK
Sbjct: 306 PLSPSTYKSYFAALLYAEDYYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVIK 365

Query: 361 TFVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFG 420
           TFVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYRI+ SN  L  KN+LLV+FG
Sbjct: 366 TFVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRISTSNYQLSMKNILLVNFG 425

Query: 421 EEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKH 480
           E+F+ RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  
Sbjct: 426 EDFHFRHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVDPYIKVTR 485

Query: 481 SSHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQ 540
           S  Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ
Sbjct: 486 SGQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITKTGEVVVGAVFQ 545

Query: 541 LYSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYN 600
           +YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN
Sbjct: 546 IYSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYN 605

Query: 601 EFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPL 658
             QECFTCP LD+L KYKII STF+SSFRLH KGLAAGHFSHIFLLDAS AIEPEVLVPL
Sbjct: 606 RDQECFTCPSLDKLHKYKIIFSTFMSSFRLHPKGLAAGHFSHIFLLDASAAIEPEVLVPL 665

BLAST of PI0000381 vs. ExPASy TrEMBL
Match: A0A6J1FJT2 (uncharacterized protein LOC111444680 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444680 PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 4.6e-243
Identity = 490/773 (63.39%), Postives = 542/773 (70.12%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   ++V DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSDVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPL--------------------------- 120
           L Y HYPNY K KESTLE+S+  S+NAKTS+ L                           
Sbjct: 66  LYYYHYPNYSKAKESTLEKSSTASYNAKTSNLLQHSSKPQSSSVFPKPSSHAAAPNCGRS 125

Query: 121 -----------------------------LLQHSSKFQSSPVFPKPSSQTV--------- 180
                                        LLQHSSK QSSPVFPKPSS            
Sbjct: 126 SSSSFGAHSLSSSTLEKSSTASCTAKTSNLLQHSSKPQSSPVFPKPSSHAAAPDCGKSNS 185

Query: 181 ----------TSLNRKSTL-----------------SSYLTPSGSI-NASSSSSSFRTNS 240
                     ++L + ST                  SS+ TPSGSI ++SSSSSSF  +S
Sbjct: 186 SSFRAHSVSSSTLEKSSTASYNAKTSSPLQHYSKPQSSHSTPSGSISSSSSSSSSFGAHS 245

Query: 241 LPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPTTSLP 300
           L  +  + Q  +YL SPSP  S LT+SSVNQLVSS+KL T TPKPS    LPS  TTS P
Sbjct: 246 LSSSLLRPQEISYLRSPSPVSSDLTRSSVNQLVSSSKLPTPTPKPS--YFLPSPSTTSFP 305

Query: 301 EPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRKPLSPL 360
           EPP    PTL   SSSK N QEYILD+++   LYL PKD+EDLIKNDIVP VLRKPLSP 
Sbjct: 306 EPP--REPTLHPTSSSKTNAQEYILDSDSLSTLYLAPKDIEDLIKNDIVPHVLRKPLSPS 365

Query: 361 TYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIKTFVAF 420
           TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE V KTFVAF
Sbjct: 366 TYKSYFAALLYAEDFYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVTKTFVAF 425

Query: 421 EIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFGEEFYA 480
           EIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYR++KSN  L   N+LLV+FGE+F++
Sbjct: 426 EIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRVSKSNYQLRMNNILLVNFGEDFHS 485

Query: 481 RHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKHSSHQQ 540
           RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  S  Q 
Sbjct: 486 RHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVNPYIEVTRSGQQL 545

Query: 541 LDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQLYSTS 600
           LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ+YSTS
Sbjct: 546 LDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITRTGEVVVGAVFQIYSTS 605

Query: 601 PNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNEFQEC 658
           PNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN  QEC
Sbjct: 606 PNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYNRDQEC 665

BLAST of PI0000381 vs. NCBI nr
Match: XP_022938442.1 (probable RNA helicase SDE3 isoform X4 [Cucurbita moschata])

HSP 1 Score: 870.9 bits (2249), Expect = 6.8e-249
Identity = 489/717 (68.20%), Postives = 541/717 (75.45%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   ++V DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSDVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L Y HYPNY K KESTLE+S+  S+NAKTS+  LLQHSSK QSSPVFPKPSS        
Sbjct: 66  LYYYHYPNYSKAKESTLEKSSTASYNAKTSN--LLQHSSKPQSSPVFPKPSSHAAAPDCG 125

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSI-NASSSSSSF 180
                         ++L + ST                  SS+ TPSGSI ++SSSSSSF
Sbjct: 126 KSNSSSFRAHSVSSSTLEKSSTASYNAKTSSPLQHYSKPQSSHSTPSGSISSSSSSSSSF 185

Query: 181 RTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPT 240
             +SL  +  + Q  +YL SPSP  S LT+SSVNQLVSS+KL T TPKPS    LPS  T
Sbjct: 186 GAHSLSSSLLRPQEISYLRSPSPVSSDLTRSSVNQLVSSSKLPTPTPKPS--YFLPSPST 245

Query: 241 TSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRKP 300
           TS PEPP    PTL   SSSK N QEYILD+++   LYL PKD+EDLIKNDIVP VLRKP
Sbjct: 246 TSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLSTLYLAPKDIEDLIKNDIVPHVLRKP 305

Query: 301 LSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIKT 360
           LSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE V KT
Sbjct: 306 LSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVTKT 365

Query: 361 FVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFGE 420
           FVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYR++KSN  L   N+LLV+FGE
Sbjct: 366 FVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRVSKSNYQLRMNNILLVNFGE 425

Query: 421 EFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKHS 480
           +F++RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  S
Sbjct: 426 DFHSRHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVNPYIEVTRS 485

Query: 481 SHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQL 540
             Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ+
Sbjct: 486 GQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITRTGEVVVGAVFQI 545

Query: 541 YSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNE 600
           YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN 
Sbjct: 546 YSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYNR 605

Query: 601 FQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLT 658
            QECF CP LDEL KYKII STF+SSFRL  KG A GHFSHIFLLDAS AIEPEVLVPLT
Sbjct: 606 DQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAPGHFSHIFLLDASAAIEPEVLVPLT 665

BLAST of PI0000381 vs. NCBI nr
Match: XP_023549845.1 (probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549846.1 probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549847.1 probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549848.1 probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549849.1 probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549850.1 probable RNA helicase SDE3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.9 bits (2236), Expect = 2.2e-247
Identity = 486/717 (67.78%), Postives = 540/717 (75.31%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   +NV DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSNVRDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L YDHYPNY K KESTLE+S+  S+NAKTS+  LLQHSSK QSSPVFPKPSS T      
Sbjct: 66  LYYDHYPNYSKAKESTLEKSSTASYNAKTSN--LLQHSSKPQSSPVFPKPSSHTAAPNCG 125

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSIN-ASSSSSSF 180
                         ++L + ST                  SS+ TPSGSI+ +SSSSSSF
Sbjct: 126 KSNSSSFRAHSLSSSTLEKTSTASYNAKTSSPLQHYSKPQSSHPTPSGSISPSSSSSSSF 185

Query: 181 RTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPT 240
             +S   +  + Q  +YL SPSP  S LT+SSVNQLVSS+KL T TPKPS   SLPS  T
Sbjct: 186 GAHSRSSSLLRPQEISYLRSPSPVSSDLTRSSVNQLVSSSKLPTPTPKPS--YSLPSPST 245

Query: 241 TSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRKP 300
           TS PEPP    PTL   SSSK N QEYILD+++   LYL PKD+EDLIKNDIVP VLRKP
Sbjct: 246 TSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLSTLYLAPKDIEDLIKNDIVPHVLRKP 305

Query: 301 LSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIKT 360
           LSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHK PNKKTK N HE V  T
Sbjct: 306 LSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLELQQITIHKRPNKKTKFNGHEKVTTT 365

Query: 361 FVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFGE 420
           FVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VY++++SN  L   N+LLV+FGE
Sbjct: 366 FVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYKVSRSNYQLRMNNILLVNFGE 425

Query: 421 EFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKHS 480
           +F++RH  T+KYDISFTFNRVCLKRAHQAI+EASDSL+Q+FLFPK+TSRKV PY +V  S
Sbjct: 426 DFHSRHRKTDKYDISFTFNRVCLKRAHQAIKEASDSLLQSFLFPKSTSRKVDPYIEVTRS 485

Query: 481 SHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQL 540
             Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ+
Sbjct: 486 GQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITKTGEVVVGAVFQI 545

Query: 541 YSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNE 600
           YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN 
Sbjct: 546 YSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYNR 605

Query: 601 FQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLT 658
            QECF CP LDEL KYKII STF+SSFRL  KG A GHFSHIFLLDAS AIEPEVLVPLT
Sbjct: 606 DQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAPGHFSHIFLLDASAAIEPEVLVPLT 665

BLAST of PI0000381 vs. NCBI nr
Match: XP_023549851.1 (probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549852.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549853.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549854.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549856.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549857.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549858.1 probable RNA helicase SDE3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.9 bits (2236), Expect = 2.2e-247
Identity = 486/717 (67.78%), Postives = 540/717 (75.31%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   +NV DSSS QK Q DTS +TPL
Sbjct: 1   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSNVRDSSSYQKIQPDTSAVTPL 60

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L YDHYPNY K KESTLE+S+  S+NAKTS+  LLQHSSK QSSPVFPKPSS T      
Sbjct: 61  LYYDHYPNYSKAKESTLEKSSTASYNAKTSN--LLQHSSKPQSSPVFPKPSSHTAAPNCG 120

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSIN-ASSSSSSF 180
                         ++L + ST                  SS+ TPSGSI+ +SSSSSSF
Sbjct: 121 KSNSSSFRAHSLSSSTLEKTSTASYNAKTSSPLQHYSKPQSSHPTPSGSISPSSSSSSSF 180

Query: 181 RTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPT 240
             +S   +  + Q  +YL SPSP  S LT+SSVNQLVSS+KL T TPKPS   SLPS  T
Sbjct: 181 GAHSRSSSLLRPQEISYLRSPSPVSSDLTRSSVNQLVSSSKLPTPTPKPS--YSLPSPST 240

Query: 241 TSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRKP 300
           TS PEPP    PTL   SSSK N QEYILD+++   LYL PKD+EDLIKNDIVP VLRKP
Sbjct: 241 TSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLSTLYLAPKDIEDLIKNDIVPHVLRKP 300

Query: 301 LSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIKT 360
           LSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHK PNKKTK N HE V  T
Sbjct: 301 LSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLELQQITIHKRPNKKTKFNGHEKVTTT 360

Query: 361 FVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFGE 420
           FVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VY++++SN  L   N+LLV+FGE
Sbjct: 361 FVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYKVSRSNYQLRMNNILLVNFGE 420

Query: 421 EFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKHS 480
           +F++RH  T+KYDISFTFNRVCLKRAHQAI+EASDSL+Q+FLFPK+TSRKV PY +V  S
Sbjct: 421 DFHSRHRKTDKYDISFTFNRVCLKRAHQAIKEASDSLLQSFLFPKSTSRKVDPYIEVTRS 480

Query: 481 SHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQL 540
             Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ+
Sbjct: 481 GQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITKTGEVVVGAVFQI 540

Query: 541 YSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYNE 600
           YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN 
Sbjct: 541 YSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYNR 600

Query: 601 FQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPLT 658
            QECF CP LDEL KYKII STF+SSFRL  KG A GHFSHIFLLDAS AIEPEVLVPLT
Sbjct: 601 DQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAPGHFSHIFLLDASAAIEPEVLVPLT 660

BLAST of PI0000381 vs. NCBI nr
Match: XP_022938443.1 (probable RNA helicase SDE3 isoform X5 [Cucurbita moschata])

HSP 1 Score: 857.1 bits (2213), Expect = 1.0e-244
Identity = 476/681 (69.90%), Postives = 524/681 (76.95%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFL IL CF CCCDDP+ DNN+ EIYTANSS R   ++V DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLHILRCFCCCCDDPDLDNNYREIYTANSSERFRVSDVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTVTSLNR 120
           L Y HYPNY K KESTLE+S+  S+NAKTSSP  LQH SK Q                  
Sbjct: 66  LYYYHYPNYSKAKESTLEKSSTASYNAKTSSP--LQHYSKPQ------------------ 125

Query: 121 KSTLSSYLTPSGSI-NASSSSSSFRTNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQL 180
               SS+ TPSGSI ++SSSSSSF  +SL  +  + Q  +YL SPSP  S LT+SSVNQL
Sbjct: 126 ----SSHSTPSGSISSSSSSSSSFGAHSLSSSLLRPQEISYLRSPSPVSSDLTRSSVNQL 185

Query: 181 VSSTKLTTSTPKPSKTSSLPSTPTTSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLP 240
           VSS+KL T TPKPS    LPS  TTS PEPP    PTL   SSSK N QEYILD+++   
Sbjct: 186 VSSSKLPTPTPKPS--YFLPSPSTTSFPEPP--REPTLHPTSSSKTNAQEYILDSDSLST 245

Query: 241 LYLVPKDVEDLIKNDIVPQVLRKPLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLEL 300
           LYL PKD+EDLIKNDIVP VLRKPLSP TYKSYFAALLYAED YYKKWS+YKL++VSLEL
Sbjct: 246 LYLAPKDIEDLIKNDIVPHVLRKPLSPSTYKSYFAALLYAEDFYYKKWSDYKLRNVSLEL 305

Query: 301 QQITIHKGPNKKTKLNSHE-VIKTFVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEG 360
           QQITIHKGPNKKTK N HE V KTFVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G
Sbjct: 306 QQITIHKGPNKKTKFNGHEKVTKTFVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQG 365

Query: 361 FVYRIAKSNCPLGKKNVLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDS 420
            VYR++KSN  L   N+LLV+FGE+F++RH  T+KYDISFTFNRVCLKRAHQAIEEASDS
Sbjct: 366 LVYRVSKSNYQLRMNNILLVNFGEDFHSRHRKTDKYDISFTFNRVCLKRAHQAIEEASDS 425

Query: 421 LIQNFLFPKATSRKVQPYFQVKHSSHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE-- 480
           L+Q+FLFPK+TSRKV PY +V  S  Q LD  QKNAI QIL L GSPPYLI+G P V   
Sbjct: 426 LLQSFLFPKSTSRKVNPYIEVTRSGQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSY 485

Query: 481 ------------------GAVFQLYSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMF 540
                             GAVFQ+YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MF
Sbjct: 486 DWAENQTRKITRTGEVVVGAVFQIYSTSPNCKILICAPRNTTCDELMISLKKVIPESNMF 545

Query: 541 RATAAFRERDEVPDDILPLCDYNEFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAA 600
           RA AAFRERDEVPDDILPLCDYN  QECF CP LDEL KYKII STF+SSFRL  KG A 
Sbjct: 546 RAIAAFRERDEVPDDILPLCDYNRDQECFACPALDELHKYKIIFSTFMSSFRLRTKGSAP 605

Query: 601 GHFSHIFLLDASEAIEPEVLVPLTKFVIDTTNVIVTGETGKQPYWVRSMIARKHGLRTSY 658
           GHFSHIFLLDAS AIEPEVLVPLTKF ID TNVIVTG+ G QPYWVRS IAR+HGL+ SY
Sbjct: 606 GHFSHIFLLDASAAIEPEVLVPLTKFAIDATNVIVTGQRGNQPYWVRSQIARRHGLKISY 658

BLAST of PI0000381 vs. NCBI nr
Match: XP_022993134.1 (probable RNA helicase SDE3 isoform X1 [Cucurbita maxima] >XP_022993136.1 probable RNA helicase SDE3 isoform X1 [Cucurbita maxima] >XP_022993137.1 probable RNA helicase SDE3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 855.5 bits (2209), Expect = 3.0e-244
Identity = 485/718 (67.55%), Postives = 533/718 (74.23%), Query Frame = 0

Query: 1   MSVFLDILCCFGCCCDDPNSDNNFHEIYTANSSARCTYTNVIDSSSCQKNQTDTSPITPL 60
           MSVFLDIL CF CCCDDP+ DNN+ EIYTANSS R   +NV DSSS QK Q DTS +TPL
Sbjct: 6   MSVFLDILRCFCCCCDDPDHDNNYREIYTANSSERFRVSNVSDSSSYQKIQPDTSAVTPL 65

Query: 61  L-YDHYPNYYKKKESTLEESNQTSHNAKTSSPLLLQHSSKFQSSPVFPKPSSQTV----- 120
           L YDHYPNY K KESTLE+S+  S+NAKTS+  L QHSSK QSSPVF KPSS        
Sbjct: 66  LYYDHYPNYSKAKESTLEKSSTASYNAKTSN--LYQHSSKPQSSPVFSKPSSHAAAPNCG 125

Query: 121 --------------TSLNRKSTL-----------------SSYLTPSGSINASSSSSSFR 180
                         ++L + ST                  SS+ TPSGSI+ SSSSSSFR
Sbjct: 126 KSSSSSFGAHSLSSSTLEKSSTASYNAKTSSLLQHSSKPQSSHPTPSGSISPSSSSSSFR 185

Query: 181 TNSLPLTPPKLQTTTYLSSPSPFFSGLTQSSVNQLVSSTKLTTSTPKPSKTSSLPSTPTT 240
            +SL  +  + Q T+YL SPSP  S L +SSVNQLVSS+KL T TPKPS   SLPS  TT
Sbjct: 186 AHSLSSSLLRPQETSYLRSPSPVSSDLPRSSVNQLVSSSKLPTPTPKPS--YSLPSPSTT 245

Query: 241 SLPEPP--PTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIKNDIVPQVLRK 300
           S PEPP   T +PT                     L LYL PKDVE+LIKNDI P VLRK
Sbjct: 246 SFPEPPREQTLHPT---------------------LTLYLAPKDVENLIKNDIEPHVLRK 305

Query: 301 PLSPLTYKSYFAALLYAEDVYYKKWSNYKLKSVSLELQQITIHKGPNKKTKLNSHE-VIK 360
           PLSP TYKSYFAALLYAED YYKKWS+YKL++VSLELQQITIHKGPNKKTK N HE VIK
Sbjct: 306 PLSPSTYKSYFAALLYAEDYYYKKWSDYKLRNVSLELQQITIHKGPNKKTKFNGHEKVIK 365

Query: 361 TFVAFEIDSVPERRPFLLSRDLVHARLCEKTLEPFEGFVYRIAKSNCPLGKKNVLLVDFG 420
           TFVAFEIDSVPERRPFLLSRDLVHARLCE+ LEPF+G VYRI+ SN  L  KN+LLV+FG
Sbjct: 366 TFVAFEIDSVPERRPFLLSRDLVHARLCERKLEPFQGLVYRISTSNYQLSMKNILLVNFG 425

Query: 421 EEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKATSRKVQPYFQVKH 480
           E+F+ RH  T+KYDISFTFNRVCLKRAHQAIEEASDSL+Q+FLFPK+TSRKV PY +V  
Sbjct: 426 EDFHFRHRKTDKYDISFTFNRVCLKRAHQAIEEASDSLLQSFLFPKSTSRKVDPYIKVTR 485

Query: 481 SSHQQLDHHQKNAIHQILHLHGSPPYLIKGPPYVE--------------------GAVFQ 540
           S  Q LD  QKNAI QIL L GSPPYLI+G P V                     GAVFQ
Sbjct: 486 SGQQLLDPAQKNAIRQILLLQGSPPYLIRGSPCVSSYDWAENQTRKITKTGEVVVGAVFQ 545

Query: 541 LYSTSPNCKILICAPRNATCDKLMISLKKVIPDSKMFRATAAFRERDEVPDDILPLCDYN 600
           +YSTSPNCKILICAPRN TCD+LMISLKKVIP+S MFRA AAFRERDEVPDDILPLCDYN
Sbjct: 546 IYSTSPNCKILICAPRNTTCDELMISLKKVIPESNMFRAIAAFRERDEVPDDILPLCDYN 605

Query: 601 EFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEPEVLVPL 658
             QECFTCP LD+L KYKII STF+SSFRLH KGLAAGHFSHIFLLDAS AIEPEVLVPL
Sbjct: 606 RDQECFTCPSLDKLHKYKIIFSTFMSSFRLHPKGLAAGHFSHIFLLDASAAIEPEVLVPL 665

BLAST of PI0000381 vs. TAIR 10
Match: AT1G05460.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 8.3e-51
Identity = 140/484 (28.93%), Postives = 256/484 (52.89%), Query Frame = 0

Query: 192 SKTSSLPSTPTTSLPEPPPTSNPTLRSASSSKKNGQEYILDANNSLPLYLVPKDVEDLIK 251
           S TS+ P + +   P+     +  ++ +  SK   + +     N LPLY +PK++ ++I+
Sbjct: 173 SLTSNRPYSRSRRAPKKDFAVDDYVKGSRPSKVVERSF----RNRLPLYEIPKEIREMIE 232

Query: 252 NDIVPQVLRKPLSPLTYKSYFAALLYAEDVYYKK-WSNYKLKSVSLELQQITIHKGPNKK 311
           N   P  L + L+   Y +Y+  LL  E++  ++    Y +++VS++ + I         
Sbjct: 233 NKEFPDDLNEGLTARNYANYYKTLLIMEELQLEEDMRAYDMENVSMKRRGI--------- 292

Query: 312 TKLNSHEVIKTFVAFEIDSVPERRPFLLSRDLVHARLC--EKTLEPFEGFVYRIAKSNCP 371
                      +++ E+  + ERRP L+  D +  R    + T   ++GFV+R+      
Sbjct: 293 -----------YLSLEVPGLAERRPSLVHGDFIFVRHAYDDGTDHAYQGFVHRV------ 352

Query: 372 LGKKNVLLVDFGEEFYARHCGTNKYDISFTFNRVCLKRAHQAIEEASDSLIQNFLFPKAT 431
             + + + + F  EF+ RH   + Y++ FT+NR+  +R +QA+ +A++ L  NFLFP   
Sbjct: 353 --EADEVHMKFASEFHQRHTAGSVYNVRFTYNRINTRRLYQAV-DAAEMLDPNFLFPSLH 412

Query: 432 SR----KVQPYFQVKHSSHQQLDHHQKNAIHQILHLHGSPPYLIKGPP------YVEGAV 491
           S     K +P+  +  +    L+  Q  +I  +L   G+PPY+I GPP       +  A+
Sbjct: 413 SGKRMIKTKPFVPISPA----LNAEQICSIEMVLGCKGAPPYVIHGPPGTGKTMTLVEAI 472

Query: 492 FQLYSTSPNCKILICAPRNATCDKL---MISLKKV-IPDSKMFRATAAFRERDEVPDDIL 551
            QLY+T  N ++L+CAP N+  D +   ++ L+ V I D+++FR  AA R  +E+  +I+
Sbjct: 473 VQLYTTQRNARVLVCAPSNSAADHILEKLLCLEGVRIKDNEIFRLNAATRSYEEIKPEII 532

Query: 552 PLCDYNEFQECFTCPELDELDKYKIILSTFISSFRLHAKGLAAGHFSHIFLLDASEAIEP 611
             C ++E    F CP L  L +YK+++ST++S+  L+A+G+  GHF+HI L +A +A EP
Sbjct: 533 RFCFFDEL--IFKCPPLKALTRYKLVVSTYMSASLLNAEGVNRGHFTHILLDEAGQASEP 592

Query: 612 EVLVPLTKFVIDTTNVIVTGETGKQPYWVRSMIARKHGLRTSYFERLGEMMPY-RSNPSF 658
           E ++ ++   +  T V++ G+  +    + S  A   GL  SY ERL E   Y   + ++
Sbjct: 593 ENMIAVSNLCLTETVVVLAGDPRQLGPVIYSRDAESLGLGKSYLERLFECDYYCEGDENY 617