PI0000110 (gene) Melon (PI 482460) v1

Overview
NamePI0000110
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProcollagen-proline 4-dioxygenase
Locationchr09: 19491366 .. 19495407 (-)
RNA-Seq ExpressionPI0000110
SyntenyPI0000110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAAAAAAGAAAAAAAGAAAAAGAAAAGAAAGATAATTTGTTTGAATTTTCTTAATTTGCAATTTCCAAAAAACCGCCCACTGATTCTAATCTTTCATTAATTGTCTTCATAGTTGAATTTTCATAATTAGTTCAAACCCATTTATATATTTTTTTTTTTCTTTTTCCTTTCTTGTAAACGAAAGAACCCTTGAATCGTTGAATTATTTTTCTTGTTCATTTCTCCGATTTGATATCGGAGAAACAATCATGGATTCTCGACCATTCCTCGCATTTTCTCTCTGCTTTCTCTCCGTCTTCACCGCCTTCGCTCGCTTGCCGGAAACGCGTACGAACAAGCAATCGTACGATCATTTTCTTCCCCTTTTCTTCGTTCTCTTTTTTTTTTTTTTTTTTTTGTAATTTCACGAATTCACTGATGCATGGTTTTGGATTCGACTGTTTTTGGAATTTTAGAAGTGGATCTGTGCTTCGATTGAAGACGGATTCATCTCCGCTCATTTTCGATCCAACACGAGTCACTCAGCTCTCCTGGCAACCCAGGTAATCTGTCTTTCTCTCTTAAGCGATCGAATAAGATGTTCCTTTACTCTCTGGCTTCATTGTGTGGAAATATTATGCTGCCTGGGATAGTCAAACCACATTTCCATCCATTCTGTTTTTAATGTATCCTCAATTGGCGATGTGTTTGTTTATATCCTTCTAAAAACTGCAAATCTACAGGGCATTTTTGTATAAGGGATTTTTATCTGATGAGGAATGTGATCACCTAATTGATCTGGTAATTGATTATGGAACGGTCTGTTTGTTTTGATTTAGATTTCGATGTTGTGGTATTTATAAATGTTTGTTTAATTCTATTATTTTCTGGAATAGGCTAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCTGGTAAAAGTGTAAGTAGTGAAGTCCGTACGAGTTCTGGCATGTTTCTTCGGAAGGCCCAGGTGCGTCAATTTGAATCATCCCATCCATGATTTTAATGTTTGGCTTTTTGAACTTATTTTTATGTTACTGTATGTAAAAGATTGTGGTGTCATGGTATGTTCTTGTAGAATTAGGTGAGAAGGATAGTGTAATTGTAGATGTCAATGTATGAAGTTACGAACGTGCTATCCAATTTCTTGGTGTTGGTAAACATTTTGTGGTAAAAACTTGAAAGGAAAACTGTCTGAACTTATGTAAATTATTTGACTACATGAATTTTCCAAAATGTGTTTGTGCAGTAATGTATGTAAGATTACCTGTTTTAGATAGAAAGAGATGCCTGAAAATAAATGAACACACGTGTTTGCCTGATGGAGTCTAGTCATTGCCCCTATGTAGTTTGACCTTATGCATGTGTTTGAGGGGCTATTGTGGAGCTATTGTGGAGGAGAATGTCAGGAACCATACGGTTACCTTTGTCCCATATCGGTTTGAATGGGATGCCCAATGTGGTACCTAAGTGGCTTGGCTCTCTGCCTCACCTTGATAGTTGGTTTTGGGGTGTGGTTATTCAAGGTGGTAATCATTGACATCCCTGTTGTCAACAATATCCCTTAGCCATCTGCCTTCTCTCCTCTTATCACCATTCATACTGCTGGAATTGGATGTTCAATTAAAGGACATTGTTGGCAACATCCTTGACTTCCTCTTCCTTTGAAATGGGTGCTTAATCTTGATGTTTAGATCCTCTCCCCCTCATTTTTCTTCTTTAAATATGATCCTGTCGGAGGATAGATCATTGACAGAACCTACAGATCTCCATTTAAAGTGTTGAAGGGTGAACAGCCTTCGTTATATTATCTCCTGTGTTTGAGGACTTGGTTTTTGGCATTTTGCTTGTTTTTAGAGAACACGATTTTCTTTGTGAATAAAACATAAGTAGAAGATATATTGAAGTTTCAAGTCTAAACAGATGGTGAAATGACCAGAAGCAATAATTATTTGTTATTTATTTAAATATACTATAATATACAAGTTTTGTTATGGGTTTAATGCCTATAAACTGCAAGTTAGTTAATTACAATCCATTACTAAATTACTGATAAACCACAAGTCTAAACTAGAGAAACATCGATGACAAAAATTAAAGATTAAATTGGTTCTGTTAGTGATAATATTGACGATGGTATTGAGAATGATCTCTAGATTATTTATTTAAGAGAAAGTGGCTATTGATAGACTTGTTTTGCATATAAATTTTTTAATATTTCAATCATGGCTTATGCCTCAAATCACCTTGAAGCTTAACTATAATGTATATGATTTATTTTGTGCTTTGAAGGATAAAATTGTTGCTGGCGTTGAAGCCAGGATAGCTGCATGGACACTCCTTCCAGCAGGTAGATTTGTTGTATGCCCATTGTCATGGATGCACCTTTTTTTTTCTTTTTTAAATTTCGGATTATAAAGCTATTCCAATCACTTACGTATCTTTCTTGGAACTATTGTTAAAATCAGAAAATGGAGAATCCATTCAAATTCTTCACTATGAGAATGGTCAAAAATATGAACCACATTTTGATTTTTTTCACGACAAGGTGAATCAGGAGTTAGGTGGCCACCGCATAGCCACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCTAATTCCGAGGTATGGCAGTGGTTCTGCTACTTCAGTGCCCTTTTTTTTTTTTTTGAAAAACGGACGTATATTTTAATTTTGTTGCCAACTGTGATGGCTTTATCTTCTGCAGTTTAAAGAATCTCAAGAAAAAGATGACAGCTGGTCTGATTGTTCTCGAAAGGGTTATGCAGGTAGGTTTGTTATTGACTCGTACAGATCATAAGTCATCAATGTATTATGTTACGTTTCCAAATTAGAAAAGGCCACAGATGGCCTATCTATCATTGATAGATCTTCCCTTGAAAGTATCTTTCTTGACCTTGACCTTCCTTTCTGGTCTTTGAGAAGGGTACTCATCGCTTGGCTAATATCCTTTATGGAGAACCAACTTGGTTTGAACCTTTGTTGTCTGATTTTCCCCTTTCCAAGAGTTGCTCGCCTGTGTCATCAATTCGTCATTATAGTCTGTTCAATAGTCTGGTTTAGAAAAAGTTTGGTTGTCCAATTAGTTTGAAATGTTAGCTGAGACTATTGCTTGTGATTTTGCATGTTCTAACTTACATTGTTTTAAAAGGCACGTCTAGACGCCATTACAAAGGCAATGCACACTCAGTAAAAGTGAAATATATTGCTTGGATGTTTACGCCTGTCTTTTAAACATATTAAACTTTGTAGGCTCTATTGTAAATTGAAATATATTTCTCGACTCTTTCATTATTAGGCTCTCTTCTATTTTTGTTGTCCTTGTTTCATGTAGCTTCTAGGATTTGATATTGTACCGTTATACATCCTACTTGGCTGTTCATTACAGTTAAAGCGCAGAAGGGCGATGCATTGTTGTTCTTCAGCCTACATCTCGACGCAACGACAGATGAAAGAAGTTTGCATGGTAGTTGCCCTGTAATTGAAGGCGAGAAATGGTCTGCAACCAAATGGATTCATGTGAGATCCTTTGAGAAGCTAACTCATGTAAGCAGGCAGGATTGCATGGACGAGAACGAAAATTGCCCGGCATGGGCGAAAAGGGGAGAGTGCAAAAAGAACCCTACTTACATGGTGGGTTCTGAAAGTGCTTTAGGATACTGTAGGAAGAGTTGCAAAGCATGCTAAAACCCTAGGAGGAAGAAGAGGGAGAAGAAGAAGAAGAAGTAATCCCCACATCTCTCTTTCTTTTTCTGTTTTGCTGAGCTTGGGTGTCGATTTTGTAATTGGCTATGTATATAACATTGGGCAGCAACTTGGTATACTATATACAATTACAAGTGGATATTAATTACATCTCTTTCATTAAACCTTGTTGTAGCAATTAACCACAAGAGTTTCATTTGATAATTTAAATGCAATTAGAAGTTTTCTCTTGTATGATGCTTATTGGCTGGTTAACTTTTCTATTCAACTTTACAAATTTT

mRNA sequence

AAAAAAGAAAAAAGAAAAAAAGAAAAAGAAAAGAAAGATAATTTGTTTGAATTTTCTTAATTTGCAATTTCCAAAAAACCGCCCACTGATTCTAATCTTTCATTAATTGTCTTCATAGTTGAATTTTCATAATTAGTTCAAACCCATTTATATATTTTTTTTTTTCTTTTTCCTTTCTTGTAAACGAAAGAACCCTTGAATCGTTGAATTATTTTTCTTGTTCATTTCTCCGATTTGATATCGGAGAAACAATCATGGATTCTCGACCATTCCTCGCATTTTCTCTCTGCTTTCTCTCCGTCTTCACCGCCTTCGCTCGCTTGCCGGAAACGCGTACGAACAAGCAATCAAGTGGATCTGTGCTTCGATTGAAGACGGATTCATCTCCGCTCATTTTCGATCCAACACGAGTCACTCAGCTCTCCTGGCAACCCAGGGCATTTTTGTATAAGGGATTTTTATCTGATGAGGAATGTGATCACCTAATTGATCTGGCTAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCTGGTAAAAGTGTAAGTAGTGAAGTCCGTACGAGTTCTGGCATGTTTCTTCGGAAGGCCCAGGATAAAATTGTTGCTGGCGTTGAAGCCAGGATAGCTGCATGGACACTCCTTCCAGCAGAAAATGGAGAATCCATTCAAATTCTTCACTATGAGAATGGTCAAAAATATGAACCACATTTTGATTTTTTTCACGACAAGGTGAATCAGGAGTTAGGTGGCCACCGCATAGCCACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCTAATTCCGAGTTTAAAGAATCTCAAGAAAAAGATGACAGCTGGTCTGATTGTTCTCGAAAGGGTTATGCAGTTAAAGCGCAGAAGGGCGATGCATTGTTGTTCTTCAGCCTACATCTCGACGCAACGACAGATGAAAGAAGTTTGCATGGTAGTTGCCCTGTAATTGAAGGCGAGAAATGGTCTGCAACCAAATGGATTCATGTGAGATCCTTTGAGAAGCTAACTCATGTAAGCAGGCAGGATTGCATGGACGAGAACGAAAATTGCCCGGCATGGGCGAAAAGGGGAGAGTGCAAAAAGAACCCTACTTACATGGTGGGTTCTGAAAGTGCTTTAGGATACTGTAGGAAGAGTTGCAAAGCATGCTAAAACCCTAGGAGGAAGAAGAGGGAGAAGAAGAAGAAGAAGTAATCCCCACATCTCTCTTTCTTTTTCTGTTTTGCTGAGCTTGGGTGTCGATTTTGTAATTGGCTATGTATATAACATTGGGCAGCAACTTGGTATACTATATACAATTACAAGTGGATATTAATTACATCTCTTTCATTAAACCTTGTTGTAGCAATTAACCACAAGAGTTTCATTTGATAATTTAAATGCAATTAGAAGTTTTCTCTTGTATGATGCTTATTGGCTGGTTAACTTTTCTATTCAACTTTACAAATTTT

Coding sequence (CDS)

ATGGATTCTCGACCATTCCTCGCATTTTCTCTCTGCTTTCTCTCCGTCTTCACCGCCTTCGCTCGCTTGCCGGAAACGCGTACGAACAAGCAATCAAGTGGATCTGTGCTTCGATTGAAGACGGATTCATCTCCGCTCATTTTCGATCCAACACGAGTCACTCAGCTCTCCTGGCAACCCAGGGCATTTTTGTATAAGGGATTTTTATCTGATGAGGAATGTGATCACCTAATTGATCTGGCTAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCTGGTAAAAGTGTAAGTAGTGAAGTCCGTACGAGTTCTGGCATGTTTCTTCGGAAGGCCCAGGATAAAATTGTTGCTGGCGTTGAAGCCAGGATAGCTGCATGGACACTCCTTCCAGCAGAAAATGGAGAATCCATTCAAATTCTTCACTATGAGAATGGTCAAAAATATGAACCACATTTTGATTTTTTTCACGACAAGGTGAATCAGGAGTTAGGTGGCCACCGCATAGCCACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCTAATTCCGAGTTTAAAGAATCTCAAGAAAAAGATGACAGCTGGTCTGATTGTTCTCGAAAGGGTTATGCAGTTAAAGCGCAGAAGGGCGATGCATTGTTGTTCTTCAGCCTACATCTCGACGCAACGACAGATGAAAGAAGTTTGCATGGTAGTTGCCCTGTAATTGAAGGCGAGAAATGGTCTGCAACCAAATGGATTCATGTGAGATCCTTTGAGAAGCTAACTCATGTAAGCAGGCAGGATTGCATGGACGAGAACGAAAATTGCCCGGCATGGGCGAAAAGGGGAGAGTGCAAAAAGAACCCTACTTACATGGTGGGTTCTGAAAGTGCTTTAGGATACTGTAGGAAGAGTTGCAAAGCATGCTAA

Protein sequence

MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSESALGYCRKSCKAC
Homology
[Close]
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.7e-129
Identity = 222/315 (70.48%), Postives = 258/315 (81.90%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPE---TRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLS 60
           MDSR FLAFSLCFL      +  P    TR++    GSV+++KT +S   FDPTRVTQLS
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLS 60

Query: 61  WQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDK 120
           W PR FLY+GFLSDEECDH I LAK KLEKSMVADN+SG+SV SEVRTSSGMFL K QD 
Sbjct: 61  WTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDD 120

Query: 121 IVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMY 180
           IV+ VEA++AAWT LP ENGES+QILHYENGQKYEPHFD+FHD+ N ELGGHRIATVLMY
Sbjct: 121 IVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMY 180

Query: 181 LSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERS 240
           LSNVEKGGET+FP  + K +Q KDDSW++C+++GYAVK +KGDALLFF+LH +ATTD  S
Sbjct: 181 LSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNS 240

Query: 241 LHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVG 300
           LHGSCPV+EGEKWSAT+WIHV+SFE+  +  +  CMDEN +C  WAK GEC+KNPTYMVG
Sbjct: 241 LHGSCPVVEGEKWSATRWIHVKSFERAFN-KQSGCMDENVSCEKWAKAGECQKNPTYMVG 300

Query: 301 SESALGYCRKSCKAC 313
           S+   GYCRKSCKAC
Sbjct: 301 SDKDHGYCRKSCKAC 314

[Close]
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 9.9e-116
Identity = 210/313 (67.09%), Postives = 241/313 (77.00%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDS+ FLAFSL  L +F+                     +  S     DPTR+TQLSW P
Sbjct: 1   MDSQYFLAFSLSLLLIFS---------------------QISSFSFSVDPTRITQLSWTP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSM-VADNESGKSVSSEVRTSSGMFLRKAQDKIV 120
           RAFLYKGFLSDEECDHLI LAK KLEKSM VAD +SG+S  SEVRTSSGMFL K QD IV
Sbjct: 61  RAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIV 120

Query: 121 AGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLS 180
           A VEA++AAWT LP ENGE++QILHYENGQKY+PHFD+F+DK   ELGGHRIATVLMYLS
Sbjct: 121 ANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLS 180

Query: 181 NVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLH 240
           NV KGGET+FPN + K  Q KDDSWS C+++GYAVK +KGDALLFF+LHL+ TTD  SLH
Sbjct: 181 NVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLH 240

Query: 241 GSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSE 300
           GSCPVIEGEKWSAT+WIHVRSF K   V    C+D++E+C  WA  GEC+KNP YMVGSE
Sbjct: 241 GSCPVIEGEKWSATRWIHVRSFGKKKLV----CVDDHESCQEWADAGECEKNPMYMVGSE 288

Query: 301 SALGYCRKSCKAC 313
           ++LG+CRKSCKAC
Sbjct: 301 TSLGFCRKSCKAC 288

[Close]
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.3e-95
Identity = 171/302 (56.62%), Postives = 220/302 (72.85%), Query Frame = 0 

Query: 14  LSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDEE 73
           +S F  F+ L ++ T+  SS SV            +P++V Q+S +PRAF+Y+GFL++ E
Sbjct: 8   ISFFAIFSVLLQSSTSLISSSSV----------FVNPSKVKQVSSKPRAFVYEGFLTELE 67

Query: 74  CDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVAGVEARIAAWTLLP 133
           CDH++ LAK  L++S VADN+SG+S  SEVRTSSG F+ K +D IV+G+E +I+ WT LP
Sbjct: 68  CDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLP 127

Query: 134 AENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSE 193
            ENGE IQ+L YE+GQKY+ HFD+FHDKVN   GGHR+AT+LMYLSNV KGGET+FP++E
Sbjct: 128 KENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAE 187

Query: 194 FKESQ---EKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHGSCPVIEGEKW 253
               +   E  +  SDC+++G AVK +KGDALLFF+LH DA  D  SLHG CPVIEGEKW
Sbjct: 188 IPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKW 247

Query: 254 SATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSESALGYCRKSCK 313
           SATKWIHV SF+++   S  +C D NE+C  WA  GEC KNP YMVG+    GYCR+SCK
Sbjct: 248 SATKWIHVDSFDRIVTPS-GNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCK 298

[Close]
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 6.9e-93
Identity = 169/275 (61.45%), Postives = 211/275 (76.73%), Query Frame = 0 

Query: 43  SSP-LIFDPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSS 102
           SSP  I +P++V Q+S +PRAF+Y+GFL+D ECDHLI LAK+ L++S VADN++G+S  S
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 103 EVRTSSGMFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDK 162
           +VRTSSG F+ K +D IV+G+E +++ WT LP ENGE +Q+L YE+GQKY+ HFD+FHDK
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 163 VNQELGGHRIATVLMYLSNVEKGGETIFPNS-EF--KESQEKDDSWSDCSRKGYAVKAQK 222
           VN   GGHRIATVL+YLSNV KGGET+FP++ EF  +   E  D  SDC++KG AVK +K
Sbjct: 147 VNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKK 206

Query: 223 GDALLFFSLHLDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEK-LTHVSRQDCMDENE 282
           G+ALLFF+L  DA  D  SLHG CPVIEGEKWSATKWIHV SF+K LTH    +C D NE
Sbjct: 207 GNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDG--NCTDVNE 266

Query: 283 NCPAWAKRGECKKNPTYMVGSESALGYCRKSCKAC 313
           +C  WA  GEC KNP YMVG+    G CR+SCKAC
Sbjct: 267 SCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.1e-65
Identity = 118/208 (56.73%), Postives = 156/208 (75.00%), Query Frame = 0 

Query: 56  LSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQ 115
           LSW+PRAF+Y  FLS EEC++LI LAK  + KS V D+E+GKS  S VRTSSG FLR+ +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 116 DKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVL 175
           DKI+  +E RIA +T +PA++GE +Q+LHYE GQKYEPH+D+F D+ N + GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 176 MYLSNVEKGGETIFPNSEFK-ESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTD 235
           MYLS+VE+GGET+FP +     S    +  S+C +KG +VK + GDALLF+S+  DAT D
Sbjct: 199 MYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLD 258

Query: 236 ERSLHGSCPVIEGEKWSATKWIHVRSFE 263
             SLHG CPVI G KWS+TKW+HV  ++
Sbjct: 259 PTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match: A0A1S3C8G4 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.2e-174
Identity = 305/316 (96.52%), Postives = 308/316 (97.47%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETR----TNKQSSGSVLRLKTDSSPLIFDPTRVTQL 60
           MDSRPFLAFSLCFLSVFTAFARLPETR    + KQS+GSVLRLKTDSSPLIFDPTRVTQL
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQSTGSVLRLKTDSSPLIFDPTRVTQL 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120
           SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120

Query: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180
           KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM
Sbjct: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180

Query: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER 240
           YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER
Sbjct: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER 240

Query: 241 SLHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMV 300
           SLHGSCPVIEGEKWSATKWIHVRSFEKL  VSRQDC+DENENCPAWAKRGECKKNPTYMV
Sbjct: 241 SLHGSCPVIEGEKWSATKWIHVRSFEKLPRVSRQDCVDENENCPAWAKRGECKKNPTYMV 300

Query: 301 GSESALGYCRKSCKAC 313
           GSE ALGYCRKSCKAC
Sbjct: 301 GSEGALGYCRKSCKAC 316

[Close]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match: A0A0A0KS38 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.1e-169
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDSRPFLAFSLCFLSVFTAFARLPETRT+KQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFLSD ECDHLIDLAKDKLEKSMVADN+SGKSVSSEVRTSSGMFLRKAQD++VA
Sbjct: 61  RAFLYKGFLSDAECDHLIDLAKDKLEKSMVADNDSGKSVSSEVRTSSGMFLRKAQDEVVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
           GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN
Sbjct: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNSEFKESQ KD+SWSDCSRKGYAVKAQKGDALLFFSL+LDATTDERSLHG
Sbjct: 181 VEKGGETIFPNSEFKESQAKDESWSDCSRKGYAVKAQKGDALLFFSLNLDATTDERSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLT-HVSRQDCMDENENCPAWAKRGECKKNPTYMVGSE 300
           SCPVI GEKWSATKWIHVRSFEK+T  VSRQ C+DENENC AWAK+GECKKNPTYMVGS 
Sbjct: 241 SCPVIAGEKWSATKWIHVRSFEKITSRVSRQGCVDENENCLAWAKKGECKKNPTYMVGSG 300

Query: 301 SALGYCRKSCKAC 313
            ALGYCRKSCKAC
Sbjct: 301 GALGYCRKSCKAC 313

[Close]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match: A0A5D3CTS4 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00740 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.8e-166
Identity = 305/375 (81.33%), Postives = 308/375 (82.13%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETR----TNKQSSGSVLRLKTDSSPLIFDPTRVTQL 60
           MDSRPFLAFSLCFLSVFTAFARLPETR    + KQS+GSVLRLKTDSSPLIFDPTRVTQL
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQSTGSVLRLKTDSSPLIFDPTRVTQL 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120
           SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120

Query: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180
           KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM
Sbjct: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180

Query: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGY------------------------ 240
           YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGY                        
Sbjct: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAGSTHRLANILYGEPTWFEPLLSD 240

Query: 241 -----------------------------------AVKAQKGDALLFFSLHLDATTDERS 300
                                              AVKAQKGDALLFFSLHLDATTDERS
Sbjct: 241 FPRVARLYYRFAIMLLGFGIVPSYTPYGSTTWLFIAVKAQKGDALLFFSLHLDATTDERS 300

Query: 301 LHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVG 313
           LHGSCPVIEGEKWSATKWIHVRSFEKL  VSRQDC+DENENCPAWAKRGECKKNPTYMVG
Sbjct: 301 LHGSCPVIEGEKWSATKWIHVRSFEKLPRVSRQDCVDENENCPAWAKRGECKKNPTYMVG 360

[Close]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match: A0A6J1BXN9 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.0e-159
Identity = 278/313 (88.82%), Postives = 294/313 (93.93%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDS  FL+FSLCFL VFTA ARLP+ R +K+ SGSVLRLK + SPLIFDPTRVTQLSWQP
Sbjct: 1   MDSPRFLSFSLCFLFVFTALARLPDMRAHKKISGSVLRLKGEPSPLIFDPTRVTQLSWQP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFLSD+ECDHLIDLAKDKLEKSMVADN SGKSVSSEVRTSSGMFL KAQD+IVA
Sbjct: 61  RAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNNSGKSVSSEVRTSSGMFLHKAQDEIVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
            VEARIAAWT LPAENGESIQILHYENGQKYEPHFD+FHDKVNQELGGHR+ATVLMYLSN
Sbjct: 121 AVEARIAAWTFLPAENGESIQILHYENGQKYEPHFDYFHDKVNQELGGHRVATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNSEFKESQEKDDSWSDC+RKGYAVKA+KGDALLFFSLHLDATTD +SLHG
Sbjct: 181 VEKGGETIFPNSEFKESQEKDDSWSDCARKGYAVKAKKGDALLFFSLHLDATTDVKSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLTHVSRQ-DCMDENENCPAWAKRGECKKNPTYMVGSE 300
           SCPVIEGEKWSATKWIHVRSFEK T  SR+ DC+DENENC +WAKRGECKKNPTYMVGSE
Sbjct: 241 SCPVIEGEKWSATKWIHVRSFEKPTRPSRRLDCVDENENCASWAKRGECKKNPTYMVGSE 300

Query: 301 SALGYCRKSCKAC 313
           SALGYCRKSC+AC
Sbjct: 301 SALGYCRKSCQAC 313

[Close]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match: A0A6J1FJ93 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111444767 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.7e-158
Identity = 277/312 (88.78%), Postives = 293/312 (93.91%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDSR FLAFSL FLSV T FARLPE  T+K+ SGSVL LK DS  LIFDPTRVTQLSWQP
Sbjct: 1   MDSRRFLAFSLFFLSVSTGFARLPE--THKKLSGSVLELKRDSPRLIFDPTRVTQLSWQP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFL+D+ECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD+IVA
Sbjct: 61  RAFLYKGFLTDQECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDEIVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
           G+EARI+AWT LP ENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN
Sbjct: 121 GIEARISAWTFLPVENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNS F ESQEKDDSWSDC+RKGYAVKAQKGDALLFFSLHLDATTD+RSLHG
Sbjct: 181 VEKGGETIFPNSAF-ESQEKDDSWSDCARKGYAVKAQKGDALLFFSLHLDATTDKRSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSES 300
           SCPVIEGEKWSATKWIHVRSF+K T +S QDC+DEN+NCP+WAKRGEC+KNPTYMVGSE 
Sbjct: 241 SCPVIEGEKWSATKWIHVRSFDKATRISSQDCVDENKNCPSWAKRGECQKNPTYMVGSEG 300

Query: 301 ALGYCRKSCKAC 313
           A+GYCRKSCKAC
Sbjct: 301 AVGYCRKSCKAC 309

[Close]
BLAST of PI0000110 vs. NCBI nr
Match: XP_008458700.1 (PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo])

HSP 1 Score: 621.3 bits (1601), Expect = 4.5e-174
Identity = 305/316 (96.52%), Postives = 308/316 (97.47%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETR----TNKQSSGSVLRLKTDSSPLIFDPTRVTQL 60
           MDSRPFLAFSLCFLSVFTAFARLPETR    + KQS+GSVLRLKTDSSPLIFDPTRVTQL
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQSTGSVLRLKTDSSPLIFDPTRVTQL 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120
           SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120

Query: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180
           KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM
Sbjct: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180

Query: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER 240
           YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER
Sbjct: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDER 240

Query: 241 SLHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMV 300
           SLHGSCPVIEGEKWSATKWIHVRSFEKL  VSRQDC+DENENCPAWAKRGECKKNPTYMV
Sbjct: 241 SLHGSCPVIEGEKWSATKWIHVRSFEKLPRVSRQDCVDENENCPAWAKRGECKKNPTYMV 300

Query: 301 GSESALGYCRKSCKAC 313
           GSE ALGYCRKSCKAC
Sbjct: 301 GSEGALGYCRKSCKAC 316

[Close]
BLAST of PI0000110 vs. NCBI nr
Match: XP_011655982.1 (probable prolyl 4-hydroxylase 7 isoform X1 [Cucumis sativus])

HSP 1 Score: 603.2 bits (1554), Expect = 1.3e-168
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDSRPFLAFSLCFLSVFTAFARLPETRT+KQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFLSD ECDHLIDLAKDKLEKSMVADN+SGKSVSSEVRTSSGMFLRKAQD++VA
Sbjct: 61  RAFLYKGFLSDAECDHLIDLAKDKLEKSMVADNDSGKSVSSEVRTSSGMFLRKAQDEVVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
           GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN
Sbjct: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNSEFKESQ KD+SWSDCSRKGYAVKAQKGDALLFFSL+LDATTDERSLHG
Sbjct: 181 VEKGGETIFPNSEFKESQAKDESWSDCSRKGYAVKAQKGDALLFFSLNLDATTDERSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLT-HVSRQDCMDENENCPAWAKRGECKKNPTYMVGSE 300
           SCPVI GEKWSATKWIHVRSFEK+T  VSRQ C+DENENC AWAK+GECKKNPTYMVGS 
Sbjct: 241 SCPVIAGEKWSATKWIHVRSFEKITSRVSRQGCVDENENCLAWAKKGECKKNPTYMVGSG 300

Query: 301 SALGYCRKSCKAC 313
            ALGYCRKSCKAC
Sbjct: 301 GALGYCRKSCKAC 313

[Close]
BLAST of PI0000110 vs. NCBI nr
Match: XP_031742194.1 (probable prolyl 4-hydroxylase 7 isoform X2 [Cucumis sativus])

HSP 1 Score: 597.0 bits (1538), Expect = 9.1e-167
Identity = 294/313 (93.93%), Postives = 303/313 (96.81%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDSRPFLAFSLCFLSVFTAFARLPETRT+KQ SGSVLRLKTDSSPLIFDPTRVTQLSWQP
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQ-SGSVLRLKTDSSPLIFDPTRVTQLSWQP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFLSD ECDHLIDLAKDKLEKSMVADN+SGKSVSSEVRTSSGMFLRKAQD++VA
Sbjct: 61  RAFLYKGFLSDAECDHLIDLAKDKLEKSMVADNDSGKSVSSEVRTSSGMFLRKAQDEVVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
           GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN
Sbjct: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNSEFKESQ KD+SWSDCSRKGYAVKAQKGDALLFFSL+LDATTDERSLHG
Sbjct: 181 VEKGGETIFPNSEFKESQAKDESWSDCSRKGYAVKAQKGDALLFFSLNLDATTDERSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLT-HVSRQDCMDENENCPAWAKRGECKKNPTYMVGSE 300
           SCPVI GEKWSATKWIHVRSFEK+T  VSRQ C+DENENC AWAK+GECKKNPTYMVGS 
Sbjct: 241 SCPVIAGEKWSATKWIHVRSFEKITSRVSRQGCVDENENCLAWAKKGECKKNPTYMVGSG 300

Query: 301 SALGYCRKSCKAC 313
            ALGYCRKSCKAC
Sbjct: 301 GALGYCRKSCKAC 312

[Close]
BLAST of PI0000110 vs. NCBI nr
Match: TYK15293.1 (putative prolyl 4-hydroxylase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 594.3 bits (1531), Expect = 5.9e-166
Identity = 305/375 (81.33%), Postives = 308/375 (82.13%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETR----TNKQSSGSVLRLKTDSSPLIFDPTRVTQL 60
           MDSRPFLAFSLCFLSVFTAFARLPETR    + KQS+GSVLRLKTDSSPLIFDPTRVTQL
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQSTGSVLRLKTDSSPLIFDPTRVTQL 60

Query: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120
           SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD 120

Query: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180
           KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM
Sbjct: 121 KIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLM 180

Query: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGY------------------------ 240
           YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGY                        
Sbjct: 181 YLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAGSTHRLANILYGEPTWFEPLLSD 240

Query: 241 -----------------------------------AVKAQKGDALLFFSLHLDATTDERS 300
                                              AVKAQKGDALLFFSLHLDATTDERS
Sbjct: 241 FPRVARLYYRFAIMLLGFGIVPSYTPYGSTTWLFIAVKAQKGDALLFFSLHLDATTDERS 300

Query: 301 LHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVG 313
           LHGSCPVIEGEKWSATKWIHVRSFEKL  VSRQDC+DENENCPAWAKRGECKKNPTYMVG
Sbjct: 301 LHGSCPVIEGEKWSATKWIHVRSFEKLPRVSRQDCVDENENCPAWAKRGECKKNPTYMVG 360

[Close]
BLAST of PI0000110 vs. NCBI nr
Match: XP_038889686.1 (probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 589.3 bits (1518), Expect = 1.9e-164
Identity = 283/312 (90.71%), Postives = 298/312 (95.51%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDSR FLAF LCFLSVFT FARLPE R+ K+SSGSV+RLKTDSSPL+FDPTRVTQLSW+P
Sbjct: 1   MDSRRFLAFCLCFLSVFTGFARLPELRSQKKSSGSVIRLKTDSSPLVFDPTRVTQLSWEP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVA 120
           RAFLYKGFLSD+ECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQD+IVA
Sbjct: 61  RAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDEIVA 120

Query: 121 GVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180
            +EARI+AWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN
Sbjct: 121 AIEARISAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSN 180

Query: 181 VEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHG 240
           VEKGGETIFPNSEFKESQEKD+SWSDC+RKGYAVKA+KGDALLFFSL  DATTD +SLHG
Sbjct: 181 VEKGGETIFPNSEFKESQEKDESWSDCARKGYAVKARKGDALLFFSLRPDATTDVKSLHG 240

Query: 241 SCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSES 300
           SCPVIEGEKWSATKWIHVRSFEK T VSRQDC+DENENC  WAKRGECKKNPTYMVGSE 
Sbjct: 241 SCPVIEGEKWSATKWIHVRSFEKATRVSRQDCVDENENCQIWAKRGECKKNPTYMVGSED 300

Query: 301 ALGYCRKSCKAC 313
           ALGYCRKSC+AC
Sbjct: 301 ALGYCRKSCRAC 312

[Close]
BLAST of PI0000110 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 463.0 bits (1190), Expect = 1.9e-130
Identity = 222/315 (70.48%), Postives = 258/315 (81.90%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPE---TRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLS 60
           MDSR FLAFSLCFL      +  P    TR++    GSV+++KT +S   FDPTRVTQLS
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLS 60

Query: 61  WQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDK 120
           W PR FLY+GFLSDEECDH I LAK KLEKSMVADN+SG+SV SEVRTSSGMFL K QD 
Sbjct: 61  WTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDD 120

Query: 121 IVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMY 180
           IV+ VEA++AAWT LP ENGES+QILHYENGQKYEPHFD+FHD+ N ELGGHRIATVLMY
Sbjct: 121 IVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMY 180

Query: 181 LSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERS 240
           LSNVEKGGET+FP  + K +Q KDDSW++C+++GYAVK +KGDALLFF+LH +ATTD  S
Sbjct: 181 LSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNS 240

Query: 241 LHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVG 300
           LHGSCPV+EGEKWSAT+WIHV+SFE+  +  +  CMDEN +C  WAK GEC+KNPTYMVG
Sbjct: 241 LHGSCPVVEGEKWSATRWIHVKSFERAFN-KQSGCMDENVSCEKWAKAGECQKNPTYMVG 300

Query: 301 SESALGYCRKSCKAC 313
           S+   GYCRKSCKAC
Sbjct: 301 SDKDHGYCRKSCKAC 314

[Close]
BLAST of PI0000110 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 436.4 bits (1121), Expect = 1.9e-122
Identity = 215/323 (66.56%), Postives = 251/323 (77.71%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPE---TRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLS 60
           MDSR FLAFSLCFL      +  P    TR++    GSV+++KT +S   FDPTRVTQLS
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLS 60

Query: 61  WQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSE-----VRTSSGMFLR 120
           W PR FLY+GFLSDEECDH I LAK KLEKSMVADN+SG+SV SE     VR SS     
Sbjct: 61  WTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFIAN 120

Query: 121 KAQ---DKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGH 180
                 D IV+ VEA++AAWT LP ENGES+QILHYENGQKYEPHFD+FHD+ N ELGGH
Sbjct: 121 MDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGH 180

Query: 181 RIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHL 240
           RIATVLMYLSNVEKGGET+FP  + K +Q KDDSW++C+++GYAVK +KGDALLFF+LH 
Sbjct: 181 RIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHP 240

Query: 241 DATTDERSLHGSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECK 300
           +ATTD  SLHGSCPV+EGEKWSAT+WIHV+SFE+  +  +  CMDEN +C  WAK GEC+
Sbjct: 241 NATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFN-KQSGCMDENVSCEKWAKAGECQ 300

Query: 301 KNPTYMVGSESALGYCRKSCKAC 313
           KNPTYMVGS+   GYCRKSCKAC
Sbjct: 301 KNPTYMVGSDKDHGYCRKSCKAC 322

[Close]
BLAST of PI0000110 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 417.9 bits (1073), Expect = 7.0e-117
Identity = 210/313 (67.09%), Postives = 241/313 (77.00%), Query Frame = 0 

Query: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQP 60
           MDS+ FLAFSL  L +F+                     +  S     DPTR+TQLSW P
Sbjct: 1   MDSQYFLAFSLSLLLIFS---------------------QISSFSFSVDPTRITQLSWTP 60

Query: 61  RAFLYKGFLSDEECDHLIDLAKDKLEKSM-VADNESGKSVSSEVRTSSGMFLRKAQDKIV 120
           RAFLYKGFLSDEECDHLI LAK KLEKSM VAD +SG+S  SEVRTSSGMFL K QD IV
Sbjct: 61  RAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIV 120

Query: 121 AGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLS 180
           A VEA++AAWT LP ENGE++QILHYENGQKY+PHFD+F+DK   ELGGHRIATVLMYLS
Sbjct: 121 ANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLS 180

Query: 181 NVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLH 240
           NV KGGET+FPN + K  Q KDDSWS C+++GYAVK +KGDALLFF+LHL+ TTD  SLH
Sbjct: 181 NVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLH 240

Query: 241 GSCPVIEGEKWSATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSE 300
           GSCPVIEGEKWSAT+WIHVRSF K   V    C+D++E+C  WA  GEC+KNP YMVGSE
Sbjct: 241 GSCPVIEGEKWSATRWIHVRSFGKKKLV----CVDDHESCQEWADAGECEKNPMYMVGSE 288

Query: 301 SALGYCRKSCKAC 313
           ++LG+CRKSCKAC
Sbjct: 301 TSLGFCRKSCKAC 288

[Close]
BLAST of PI0000110 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-96
Identity = 171/302 (56.62%), Postives = 220/302 (72.85%), Query Frame = 0 

Query: 14  LSVFTAFARLPETRTNKQSSGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDEE 73
           +S F  F+ L ++ T+  SS SV            +P++V Q+S +PRAF+Y+GFL++ E
Sbjct: 8   ISFFAIFSVLLQSSTSLISSSSV----------FVNPSKVKQVSSKPRAFVYEGFLTELE 67

Query: 74  CDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLRKAQDKIVAGVEARIAAWTLLP 133
           CDH++ LAK  L++S VADN+SG+S  SEVRTSSG F+ K +D IV+G+E +I+ WT LP
Sbjct: 68  CDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLP 127

Query: 134 AENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSE 193
            ENGE IQ+L YE+GQKY+ HFD+FHDKVN   GGHR+AT+LMYLSNV KGGET+FP++E
Sbjct: 128 KENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAE 187

Query: 194 FKESQ---EKDDSWSDCSRKGYAVKAQKGDALLFFSLHLDATTDERSLHGSCPVIEGEKW 253
               +   E  +  SDC+++G AVK +KGDALLFF+LH DA  D  SLHG CPVIEGEKW
Sbjct: 188 IPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKW 247

Query: 254 SATKWIHVRSFEKLTHVSRQDCMDENENCPAWAKRGECKKNPTYMVGSESALGYCRKSCK 313
           SATKWIHV SF+++   S  +C D NE+C  WA  GEC KNP YMVG+    GYCR+SCK
Sbjct: 248 SATKWIHVDSFDRIVTPS-GNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCK 298

[Close]
BLAST of PI0000110 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 342.0 bits (876), Expect = 4.9e-94
Identity = 169/275 (61.45%), Postives = 211/275 (76.73%), Query Frame = 0 

Query: 43  SSP-LIFDPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSS 102
           SSP  I +P++V Q+S +PRAF+Y+GFL+D ECDHLI LAK+ L++S VADN++G+S  S
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 103 EVRTSSGMFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDK 162
           +VRTSSG F+ K +D IV+G+E +++ WT LP ENGE +Q+L YE+GQKY+ HFD+FHDK
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 163 VNQELGGHRIATVLMYLSNVEKGGETIFPNS-EF--KESQEKDDSWSDCSRKGYAVKAQK 222
           VN   GGHRIATVL+YLSNV KGGET+FP++ EF  +   E  D  SDC++KG AVK +K
Sbjct: 147 VNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKK 206

Query: 223 GDALLFFSLHLDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEK-LTHVSRQDCMDENE 282
           G+ALLFF+L  DA  D  SLHG CPVIEGEKWSATKWIHV SF+K LTH    +C D NE
Sbjct: 207 GNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDG--NCTDVNE 266

Query: 283 NCPAWAKRGECKKNPTYMVGSESALGYCRKSCKAC 313
           +C  WA  GEC KNP YMVG+    G CR+SCKAC
Sbjct: 267 SCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

The following BLAST results are available for this feature:
BLAST of PI0000110 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L9702.7e-12970.48Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4J0A89.9e-11667.09Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q8LAN34.3e-9556.62Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
F4JAU36.9e-9361.45Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q9LN206.1e-6556.73Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
BLAST of PI0000110 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3C8G42.2e-17496.52Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 S... [more]
A0A0A0KS386.1e-16994.25Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=... [more]
A0A5D3CTS42.8e-16681.33Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1BXN92.0e-15988.82Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412... [more]
A0A6J1FJ933.7e-15888.78Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111444767 ... [more]
BLAST of PI0000110 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008458700.14.5e-17496.52PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo][more]
XP_011655982.11.3e-16894.25probable prolyl 4-hydroxylase 7 isoform X1 [Cucumis sativus][more]
XP_031742194.19.1e-16793.93probable prolyl 4-hydroxylase 7 isoform X2 [Cucumis sativus][more]
TYK15293.15.9e-16681.33putative prolyl 4-hydroxylase 7 [Cucumis melo var. makuwa][more]
XP_038889686.11.9e-16490.71probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida][more]
BLAST of PI0000110 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G28480.11.9e-13070.48Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.21.9e-12266.56Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.17.0e-11767.09Oxoglutarate/iron-dependent oxygenase [more]
AT5G18900.13.1e-9656.622-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G06300.14.9e-9461.45P4H isoform 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 271..312
e-value: 8.0E-5
score: 32.0
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 272..312
score: 9.699209
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 60..257
e-value: 5.7E-55
score: 198.6
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 141..257
e-value: 5.7E-20
score: 72.0
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 52..258
e-value: 2.7E-77
score: 261.1
NoneNo IPR availablePANTHERPTHR10869:SF140OS03G0803500 PROTEINcoord: 41..312
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 41..312
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 136..258
score: 12.478646

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000110.1PI0000110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen