Moc11g12950 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g12950
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Calcium-binding EF-hand family protein
Location	chr11: 9591588 .. 9592067 (+)
RNA-Seq Expression	Moc11g12950
Synteny	Moc11g12950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAATCCTGAGGCGATGTCTCCATTAACCGCCTCCGATTTGCAGAGGATCTTCGAGACGCTCGACAGTAATGGCGACGGATTCTTGAGCGTGGAGGAGCTCAATCGCCTGCTCGACAGCATCGGGGATGGCCAAATCTGCGACGATCTCGAGTCGTTGATCGGCAAATGCAAGCTTGATTTTGAAGAATTCTTGTCTCTATACAAATCCATATCGGAGAGAAACAGAGCAGGCGCGGAAGAGGAAATTGAGAGCGATCTTTTGAATGCGTTCCAAGTGTTCGATCAAAATCGCGATGGATTTATTTGTTGCGAGGAGCTTCGGAATGTGCTGGCGAGGCTAGGGCTGTGGGATGAGCGCTGCGGCCGCGATTGCAGCAGCATGATTGGCGCATTCGATACAAATTTCGATGGACGGCTTGATTTTCAGGAGTTCAAGAACATAATGCTCTCTGCTTCCAATTCTCCATGTATTTAA

mRNA sequence

ATGTTGAATCCTGAGGCGATGTCTCCATTAACCGCCTCCGATTTGCAGAGGATCTTCGAGACGCTCGACAGTAATGGCGACGGATTCTTGAGCGTGGAGGAGCTCAATCGCCTGCTCGACAGCATCGGGGATGGCCAAATCTGCGACGATCTCGAGTCGTTGATCGGCAAATGCAAGCTTGATTTTGAAGAATTCTTGTCTCTATACAAATCCATATCGGAGAGAAACAGAGCAGGCGCGGAAGAGGAAATTGAGAGCGATCTTTTGAATGCGTTCCAAGTGTTCGATCAAAATCGCGATGGATTTATTTGTTGCGAGGAGCTTCGGAATGTGCTGGCGAGGCTAGGGCTGTGGGATGAGCGCTGCGGCCGCGATTGCAGCAGCATGATTGGCGCATTCGATACAAATTTCGATGGACGGCTTGATTTTCAGGAGTTCAAGAACATAATGCTCTCTGCTTCCAATTCTCCATGTATTTAA

Coding sequence (CDS)

ATGTTGAATCCTGAGGCGATGTCTCCATTAACCGCCTCCGATTTGCAGAGGATCTTCGAGACGCTCGACAGTAATGGCGACGGATTCTTGAGCGTGGAGGAGCTCAATCGCCTGCTCGACAGCATCGGGGATGGCCAAATCTGCGACGATCTCGAGTCGTTGATCGGCAAATGCAAGCTTGATTTTGAAGAATTCTTGTCTCTATACAAATCCATATCGGAGAGAAACAGAGCAGGCGCGGAAGAGGAAATTGAGAGCGATCTTTTGAATGCGTTCCAAGTGTTCGATCAAAATCGCGATGGATTTATTTGTTGCGAGGAGCTTCGGAATGTGCTGGCGAGGCTAGGGCTGTGGGATGAGCGCTGCGGCCGCGATTGCAGCAGCATGATTGGCGCATTCGATACAAATTTCGATGGACGGCTTGATTTTCAGGAGTTCAAGAACATAATGCTCTCTGCTTCCAATTCTCCATGTATTTAA

Protein sequence

MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI

Homology

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BLAST of Moc11g12950 vs. NCBI nr
Match: XP_022139705.1 (probable calcium-binding protein CML44 [Momordica charantia])

HSP 1 Score: 325.9 bits (834), Expect = 2.0e-85
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL 60
           MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL
Sbjct: 1   MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL 60

Query: 61  DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE 120
           DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE
Sbjct: 61  DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE 120

Query: 121 RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI 160
           RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI
Sbjct: 121 RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI 159

[Close]

BLAST of Moc11g12950 vs. NCBI nr
Match: KAG7025009.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 214.9 bits (546), Expect = 4.9e-52
Identity = 109/151 (72.19%), Postives = 124/151 (82.12%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK  +DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+NRDGFI C+EL NVL RLGLWD+ RC RD
Sbjct: 61  SLYESISEMNRGG--EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDRRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +SNS
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNS 149

[Close]

BLAST of Moc11g12950 vs. NCBI nr
Match: XP_022976028.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 214.5 bits (545), Expect = 6.4e-52
Identity = 109/151 (72.19%), Postives = 125/151 (82.78%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK K+DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSKIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+NRDGFI C+EL NVL RLGLWD+ RC RD
Sbjct: 61  SLYESISEINRGG--EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDKRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +S+S
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSHS 149

[Close]

BLAST of Moc11g12950 vs. NCBI nr
Match: XP_023536413.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.2 bits (544), Expect = 8.4e-52
Identity = 109/151 (72.19%), Postives = 124/151 (82.12%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK  +DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+NRDGFI C+EL NVL RLGLWD+ RC RD
Sbjct: 61  SLYESISEINRGG--EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDRRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +SNS
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNS 149

[Close]

BLAST of Moc11g12950 vs. NCBI nr
Match: KAG6592136.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.9e-51
Identity = 108/151 (71.52%), Postives = 123/151 (81.46%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK  +DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+N DGFI C+EL NVL RLGLWD+ RC RD
Sbjct: 61  SLYESISEMNRGG--EEIESDLLNAFQVFDENHDGFISCDELHNVLVRLGLWDDKRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +SNS
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNS 149

[Close]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 5.3e-25
Identity = 62/148 (41.89%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 10  LTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQ-ICDDLESLIGKCKLDFEEFLSL 69
           +T +DL+R+F+TLD N DG ++++EL  +LD +G  +   D+LE ++GK  LD +EFL  
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 70  Y-----KSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCG 129
           Y      S   +       + +  +  AF VFD N DG+I  EELR+VL RLG  +E   
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKA 125

Query: 130 RDCSSMIGAFDTNFDGRLDFQEFKNIML 152
            DC  MI   D N DG +DF+EFKN++L
Sbjct: 126 WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.8e-23
Identity = 65/144 (45.14%), Postives = 85/144 (59.03%), Query Frame = 0


Query: 13  SDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL------DFEEFL 72
           ++L RIF+  D NGDG ++ +ELN  L+++G      DL  +I K  L      D EEF 
Sbjct: 4   AELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFG 63

Query: 73  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDC 132
            LY++I E      E + E D+  AF VFDQNRDGFI  EELR+VLA LGL   R   DC
Sbjct: 64  GLYQTIME------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDC 123

Query: 133 SSMIGAFDTNFDGRLDFQEFKNIM 151
             MI   D + DG ++F+EFK +M
Sbjct: 124 KRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.8e-23
Identity = 67/159 (42.14%), Postives = 90/159 (56.60%), Query Frame = 0


Query: 10  LTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLI------GKCKLDFE 69
           L A  LQRIF+  D NGDGF++VEEL++ L  +G      DL+S +      G   L+F+
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 70  EFLSLYKSISERNRAGA------------EEEIESDLLNAFQVFDQNRDGFICCEELRNV 129
           +F SL+K++ +    GA              E ESDL  AF+VFD+N DGFI   EL+ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 130 LARLGLWDERCGRDCSSMIGAFDTNFDGRLDFQEFKNIM 151
           L +LGL +         MI + D N DGR+DF EFKN+M
Sbjct: 145 LKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.2e-21
Identity = 65/152 (42.76%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 12  ASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCK------LDFEEF 71
           A++L R+FE  D NGDG ++ EEL   L  +G     D+L ++I +        +D EEF
Sbjct: 90  AAELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEF 149

Query: 72  LSLYKSI------SERNRAGAEEEIE-SDLLNAFQVFDQNRDGFICCEELRNVLARLGLW 131
             LY+SI      S+  RA  EEE E  D+  AF+VFD N DG+I  +EL  VLA LGL 
Sbjct: 150 GELYRSIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLK 209

Query: 132 DERCGRDCSSMIGAFDTNFDGRLDFQEFKNIM 151
             R   +C  MIG  D + DGR+DF EF  +M
Sbjct: 210 QGRTAEECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 6.1e-21
Identity = 59/144 (40.97%), Postives = 86/144 (59.72%), Query Frame = 0


Query: 13  SDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCK------LDFEEFL 72
           S+L+R+F+  D NGDG ++ EELN  L+++G      DL  +I K        +D +EF 
Sbjct: 64  SELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFE 123

Query: 73  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDC 132
           SLY SI + +    E E E D+ +AF VFDQ+ DGFI  EEL++V+A LGL   +    C
Sbjct: 124 SLYSSIVDEHHNDGETE-EEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGC 183

Query: 133 SSMIGAFDTNFDGRLDFQEFKNIM 151
             MI   D + DGR++++EF  +M
Sbjct: 184 KKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of Moc11g12950 vs. ExPASy TrEMBL
Match: A0A6J1CD20 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111010552 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 9.6e-86
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL 60
           MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL
Sbjct: 1   MLNPEAMSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL 60

Query: 61  DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE 120
           DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE
Sbjct: 61  DFEEFLSLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE 120

Query: 121 RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI 160
           RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI
Sbjct: 121 RCGRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNSPCI 159

[Close]

BLAST of Moc11g12950 vs. ExPASy TrEMBL
Match: A0A6J1IKW9 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111476552 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.1e-52
Identity = 109/151 (72.19%), Postives = 125/151 (82.78%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK K+DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSKIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+NRDGFI C+EL NVL RLGLWD+ RC RD
Sbjct: 61  SLYESISEINRGG--EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDKRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +S+S
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSHS 149

[Close]

BLAST of Moc11g12950 vs. ExPASy TrEMBL
Match: A0A6J1FD73 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111443045 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.5e-51
Identity = 108/151 (71.52%), Postives = 123/151 (81.46%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSP  ASDLQRIFET+D NGDGF+S+EEL+ LL+  G   I DDLESLIGK  +DF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 67  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDE-RCGRD 126
           SLY+SISE NR G  EEIESDLLNAFQVFD+NRDGFI C+EL NVL RL LWD+ RC RD
Sbjct: 61  SLYESISEINRGG--EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLELWDDRRCVRD 120

Query: 127 CSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 157
           C  MIGAFD + DGRLDF+EFKN+M  +SNS
Sbjct: 121 CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNS 149

[Close]

BLAST of Moc11g12950 vs. ExPASy TrEMBL
Match: A0A6P5ZL18 (probable calcium-binding protein CML44 OS=Durio zibethinus OX=66656 GN=LOC111301784 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.5e-41
Identity = 96/165 (58.18%), Postives = 115/165 (69.70%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66
           MSPL+ +DLQRIFE LD NGDGF+S+EELN LL+ IG     ++LESL+GK  LDF+EFL
Sbjct: 28  MSPLSKNDLQRIFEKLDKNGDGFVSLEELNWLLERIGVQFSLEELESLVGKPFLDFDEFL 87

Query: 67  SLYKSISERNR----------AGAEEEIE----------SDLLNAFQVFDQNRDGFICCE 126
             Y SIS +N           AG+EEE+E          SDL  AF+VFD N DGFI CE
Sbjct: 88  FFYYSISNQNADGGKKKIEPVAGSEEELELIDSHGGEEDSDLAKAFKVFDLNGDGFISCE 147

Query: 127 ELRNVLARLGLWDERCGRDCSSMIGAFDTNFDGRLDFQEFKNIML 152
           EL+NVL RLGLWDE  G+DC SMI  +DTN DG +DF+EFKN+ML
Sbjct: 148 ELQNVLGRLGLWDESSGKDCRSMICFYDTNMDGMVDFEEFKNMML 192

[Close]

BLAST of Moc11g12950 vs. ExPASy TrEMBL
Match: A0A2N9GA67 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24170 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.7e-40
Identity = 89/152 (58.55%), Postives = 112/152 (73.68%), Query Frame = 0


Query: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQIC-DDLESLIGKCKLDFEEF 66
           MSP+  +DL RIFE LD NGDG +S+EELN LL+ IG   I  ++LES++GK  LD  EF
Sbjct: 1   MSPINTNDLHRIFEKLDKNGDGLVSLEELNWLLEKIGVQIISQEELESIVGKSSLDLNEF 60

Query: 67  LSLYKSISERNRAGA-------EEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLW 126
           LS + SIS++N + +       EE  ESDL+ AF+VFD N DGFI C+EL+NVL+RLGLW
Sbjct: 61  LSFHDSISKQNSSCSNEVVEEEEENEESDLVKAFKVFDLNGDGFISCDELQNVLSRLGLW 120

Query: 127 DERCGRDCSSMIGAFDTNFDGRLDFQEFKNIM 151
           DE  GRDC SMI  +DTN DG+LDF+EFKN+M
Sbjct: 121 DENSGRDCRSMIHVYDTNLDGQLDFEEFKNMM 152

[Close]

BLAST of Moc11g12950 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 115.5 bits (288), Expect = 3.8e-26
Identity = 62/148 (41.89%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 10  LTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQ-ICDDLESLIGKCKLDFEEFLSL 69
           +T +DL+R+F+TLD N DG ++++EL  +LD +G  +   D+LE ++GK  LD +EFL  
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 70  Y-----KSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCG 129
           Y      S   +       + +  +  AF VFD N DG+I  EELR+VL RLG  +E   
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKA 125

Query: 130 RDCSSMIGAFDTNFDGRLDFQEFKNIML 152
            DC  MI   D N DG +DF+EFKN++L
Sbjct: 126 WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Moc11g12950 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 109.4 bits (272), Expect = 2.7e-24
Identity = 65/144 (45.14%), Postives = 85/144 (59.03%), Query Frame = 0


Query: 13  SDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKL------DFEEFL 72
           ++L RIF+  D NGDG ++ +ELN  L+++G      DL  +I K  L      D EEF 
Sbjct: 4   AELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFG 63

Query: 73  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDC 132
            LY++I E      E + E D+  AF VFDQNRDGFI  EELR+VLA LGL   R   DC
Sbjct: 64  GLYQTIME------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDC 123

Query: 133 SSMIGAFDTNFDGRLDFQEFKNIM 151
             MI   D + DG ++F+EFK +M
Sbjct: 124 KRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Moc11g12950 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 109.4 bits (272), Expect = 2.7e-24
Identity = 67/159 (42.14%), Postives = 90/159 (56.60%), Query Frame = 0


Query: 10  LTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLI------GKCKLDFE 69
           L A  LQRIF+  D NGDGF++VEEL++ L  +G      DL+S +      G   L+F+
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 70  EFLSLYKSISERNRAGA------------EEEIESDLLNAFQVFDQNRDGFICCEELRNV 129
           +F SL+K++ +    GA              E ESDL  AF+VFD+N DGFI   EL+ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 130 LARLGLWDERCGRDCSSMIGAFDTNFDGRLDFQEFKNIM 151
           L +LGL +         MI + D N DGR+DF EFKN+M
Sbjct: 145 LKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Moc11g12950 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 102.1 bits (253), Expect = 4.3e-22
Identity = 59/144 (40.97%), Postives = 86/144 (59.72%), Query Frame = 0


Query: 13  SDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCK------LDFEEFL 72
           S+L+R+F+  D NGDG ++ EELN  L+++G      DL  +I K        +D +EF 
Sbjct: 64  SELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFE 123

Query: 73  SLYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDC 132
           SLY SI + +    E E E D+ +AF VFDQ+ DGFI  EEL++V+A LGL   +    C
Sbjct: 124 SLYSSIVDEHHNDGETE-EEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGC 183

Query: 133 SSMIGAFDTNFDGRLDFQEFKNIM 151
             MI   D + DGR++++EF  +M
Sbjct: 184 KKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of Moc11g12950 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 101.7 bits (252), Expect = 5.7e-22
Identity = 58/143 (40.56%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 14  DLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKC------KLDFEEFLS 73
           +L R+F+  D NGDG ++  EL     S+G     +++  +I K        +D +EF S
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 74  LYKSISERNRAGAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLWDERCGRDCS 133
           LY+ + E      E+E E D+  AF+VFDQN DGFI  EELR+VLA +GL   R   DC 
Sbjct: 65  LYQEMVE------EKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCK 124

Query: 134 SMIGAFDTNFDGRLDFQEFKNIM 151
            MI   D + DG ++F+EFK +M
Sbjct: 125 KMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of Moc11g12950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139705.1	2.0e-85	100.00	probable calcium-binding protein CML44 [Momordica charantia]	[more]
KAG7025009.1	4.9e-52	72.19	Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022976028.1	6.4e-52	72.19	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
XP_023536413.1	8.4e-52	72.19	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
KAG6592136.1	1.9e-51	71.52	Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc11g12950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	5.3e-25	41.89	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SRR7	3.8e-23	45.14	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9SVG9	3.8e-23	42.14	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q0JC44	1.2e-21	42.76	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O22845	6.1e-21	40.97	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]

BLAST of Moc11g12950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CD20	9.6e-86	100.00	probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IKW9	3.1e-52	72.19	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114765...	[more]
A0A6J1FD73	3.5e-51	71.52	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6P5ZL18	1.5e-41	58.18	probable calcium-binding protein CML44 OS=Durio zibethinus OX=66656 GN=LOC111301...	[more]
A0A2N9GA67	2.7e-40	58.55	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24170 PE=4 SV=1	[more]

BLAST of Moc11g12950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	3.8e-26	41.89	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	2.7e-24	45.14	ARF-GAP domain 11	[more]
AT4G20780.1	2.7e-24	42.14	calmodulin like 42	[more]
AT2G43290.1	4.3e-22	40.97	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	5.7e-22	40.56	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 e-value: 2.8E-4 score: 30.2 coord: 87..115 e-value: 0.0012 score: 28.1 coord: 125..153 e-value: 0.76 score: 18.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 15..38 e-value: 3.5E-6 score: 26.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 86..150 e-value: 1.1E-9 score: 38.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 83..118 score: 13.871811
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 121..156 score: 9.185384
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 10..45 score: 13.481276
None	No IPR available	GENE3D	1.10.238.10		coord: 73..157 e-value: 3.0E-17 score: 65.1
None	No IPR available	GENE3D	1.10.238.10		coord: 2..72 e-value: 1.1E-9 score: 40.4
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 7..156
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 7..156
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 96..108
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 12..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g12950.1	Moc11g12950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding