Moc10g13830 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g13830
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionreactive Intermediate Deaminase A, chloroplastic-like
Locationchr10: 10500289 .. 10504085 (+)
RNA-Seq ExpressionMoc10g13830
SyntenyMoc10g13830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTGGTGTGCGGCTCGGACCTTCCACATGCCGGCGTTCGACGTCACTGCGCTCCGCTCCAAGACCCCGCTAGCCGTCGGCGTCGGTTGCGCTTCCGTCGCCGGCACCACCTTGTGGCGATCTTCATCAACTTCCCCTGCTTCCAAGCGCCAAATGCCGTTCGCATCCCTTGGCATTTCCGCGGATACTTGTACGCCCTTTCTTCTCTCCTTTTTTTTTTTTTTTCTTTTCGTTTTCTGTTCAGCTGCTGAGAGAATTTTCGCTGCTATCTCAGAGAAATCGGATTGTTAATTACCTTTTTCTATTGTCCTTGGACGGATTTTGTATGGTTTGATTTGAGTTTCTACTGACAAGTTATTAAAGCTCTGATATCTGGCCTTTCTTTGGGATTAGATTTAACGCTTGGTGGTGAAATAATAGATTATTGGTATTCTTTCCCCTTTCTGTGCAAGAAAACTGTAATATCGGGAAACATTGTAATTTGTACGAAATGTGCAGCTAATTCAGTTCTTTTCATCTTAAAATTAGAAACCTCATCGTTAACACTCTAGAATGGTATGGTGCAGGCAGCGGGTATAAACTTGGGCATTCACATCCTAAATCACCAAAATAATTTTGAGTTAGAATTATGTACTTGATTTAAAATTTTAATTTTGATCATCCATTTTTAACTAAAAACTAATTAATTTCTAAATGTGATATGAAGATATGTATTGAACACAATATTATTAGTCAGTTCAACTTACTCGTACAAAATTAGTTCTTCATAATAATAAATAAACAAAAATCTGATGGGCAACCAAGACTTTCAGTGCACCATACGTATACAAGGAGGATGTTGGATAATGTCGGGAATAAAGGGGACAAGGTGTAGGACAGCGGTTATGAGAGAGTATGCGGAGTGCTGTATACTTGTCCTTCCCTGATTGTAATGTGCTAGAATGGGAATAGGCCACATAGGGATCAGCGTATTTGACGTGGGCTTGGAGCCTTGCATGTGTATCAGATTATTTTGTGTTTTTGGCTTAGAATTTCCTTCCAACGAAGAGAGCATCTGACCTCTCTAAGAGTTAGTAGACTTGTTCTTGCTGATTTTATTTCGTTAAATGAATTTTCATCAATAGGCCTTTTCGGTTCCCATCAAAATCTAAATAGGCATTAAGTAAATTCAAATAGACAACACTTATTCAATTGCCTCCAAGTGTTTTGGAGATATACAGGCTGTTAGTATCTCTTTTTGGATAGTTTTCCTTCCCTGATTGTTGTCGTGTGCTGAATGTTTCAAAACAGACGCATAACCACCACTTAATGTCAACGTCTTTTGTGGCAGGTATCAAGGAAGCTGTTCAGACTGACAAGGCTCCAGCAGCATTAGGGCCATATTCTCAGGCTATCAAAGCCAACAACCTTCTCTTTGTGTCTGGTGTCCTAGGTCTAAATCCTGAGGTAGCTTCATCACTTTATGATTGTTATTCCCTTTTATTTGATTGTGCTTACGTGTTGATTCAACCTAAACATTGCTCGAGGGATTAGGGTTAGACATTAGTGCCTCCAGTATAAATCTCACAATCTTGATTTGTAATTGATATTGTAAAAAGGAAAAAAAAGAAGAAGGATGAAGTGGGTTTCTCTTTGTGTGCGTGTGCATGTTGTCATGATGTCTAACATAATGTGTGTCCTCATGTTTGAATGCAGACAGGGAAATTTGTCTCAGATGATGTTGAAGGCCAAACTGAGCAGGTATATCCTTGTCCTTGTAGATCAAAATTTTGAGCTGAATATCAAAACTTCTAAGTTAATGAAATATTGCAGGTCCTCAAAAATATGGGTGAAATATTGAAAGCTGGAGGTGCTAGCTATGCTTCAGTGGTTAAGACGACCATTATGTATGAAAATTTGGCCTCTTTGCTTTATGATCTTAATATTTGAAGAGTAGGGAGTTGTTTGGTAGTTGTTCTAAAAAATGGTTTCATGGTATTGTCTACTTTTAGGAACAACATTTGAGTGGCTGATAAAAGTTAAGATTGTTTGACCCAACAAGCACTATTTACAATCTCATAATTTTGGAATGAAGAACAAATTTAGTTATTCTAAAGGTCTAGGTCATATTTCCCAATTTTTAGTAGCTATTCTCTAAATGATCTTATTACAAGTTGGAAAGCTCTCTTATAATTGCCCATGTGGCCTTTTGTATATTTCATACCATCAATGAAATATTTGTTTCATATCAAAAAAAAAAAAAAAAAAGGTCTAGGTCCGATTTTAGTCTAAAAAATTTCATGTTTATTGTATTTTACTTCCATATTTATCAACCTTTTGTTTTAGCCTCTTACACTTTATATTAAAAGTCATTTTAGTCTTTATAATTATTTTTGCATAAATCACTTTATGGAAGCTTGACAAAGTATATCTTTTGATAATTTACTTGATGAGTAGACGTCCATGTGAACAAGTAGTATTTGAAAGTAGATGTAATAAGTTATTTTTTGAGGATCAAAATGGATGAAGACTTTTGTTAAATAATTAATTGAAAATCAATGATATGAATGAAAAATACGTTTATAGAAGAGTGTAGGGACTAGAATGTTTAAAATTCAGAGACTTGAAGAATGAGTATCAAAAGTTATGGACAAAAATAGGATTCTATTCTAAGAAATATGTCATCTTTATGAGAGAACAATTCTTAGACGATATTGATAGAAACTCCTTTTTATTATTTATGATTAGAGGGATTACAAGGGTCTAGGGAGAATTGGAGAGCTCCCTGATCTCTCCCAAACATTCCTACAGACAATAGCCTGAAAAGATAAAACACCTACCTCTCTACAAGGAGTATATACCTTAACAGCATGCCCAAAATACCCTTTCTTACCCGTACACTGGTCCCTCCCCTCTAACAGAATTCTTACTTTTTCTCTGCTATACCCGCGTGTCTCTTCCTCCTATAGTAAACGTTCATCACCCTAGGTCTATCAGATATTTACCTAGACCCATTTTCAATAATGGATTGTTCTAAAAGTATTGAAAAAGATCTTTCAGAACATAACCAAGCAGCCCTACGTTTTTCCACTGATTCAGTTATTTATTCACAATTATTTCTTGCAGGTTGGCTGATCTGAAGGATTTCAAGAAAGTAAACGAGATTTATGCTAAATGTAAGTTTTGTTAAACTTAAACTGAATCTCTAATCTCTCAGTTATCGGATGCTGAGCATTTAGCTTAGATATGCCTTTATGATCACCCTCTGTGCCCATTACAGTTCAACTTTAGTTTCTAGAGTATAGTTATTTTAGTAAATTTAAAATTTGCATCTTCGATGAATTTGTATATGATTCTACTTGGAGAATAAGATGAACTTTTCTTTTTACCTATTTTTCTGGCTGTGTGAAATTACCCACATGGTGGTTACTTCGATACTTTGATATATTGGTTCATGGTTTTCTTTTGATCCAATTGTTGCGGGTCAGTTGATGAAAAGTGGATGTTCTATTTACTGAAGGGTAATTGAAATCACGATGAATTGATGATTCTATGATTCTATCCATATTGCTTTGATATATTCTCTTGAGATAAGAATGTATTCTTCAACTAAAAAATCTGTGATTTCCTCAGATTGATGACATTAACTTCATTTTTAGTGACTTCAAGCTGAAGTTGACATGGTTTTCACATTGGTTTTGTTTGCTAATTTTTATGCAGACTTTCTGTCCCCTGCTCCAGCTCGGTCAACATTTCAGGTTGCAGCATTGCCTTTGGATGCTAAGGTTGAAATCGAGTGCATCGCTACACTTTGA

mRNA sequence

ATGGCTTGGTGTGCGGCTCGGACCTTCCACATGCCGGCGTTCGACGTCACTGCGCTCCGCTCCAAGACCCCGCTAGCCGTCGGCGTCGGTTGCGCTTCCGTCGCCGGCACCACCTTGTGGCGATCTTCATCAACTTCCCCTGCTTCCAAGCGCCAAATGCCGTTCGCATCCCTTGGCATTTCCGCGGATACTTGTATCAAGGAAGCTGTTCAGACTGACAAGGCTCCAGCAGCATTAGGGCCATATTCTCAGGCTATCAAAGCCAACAACCTTCTCTTTGTGTCTGGTGTCCTAGGTCTAAATCCTGAGACAGGGAAATTTGTCTCAGATGATGTTGAAGGCCAAACTGAGCAGGTCCTCAAAAATATGGGTGAAATATTGAAAGCTGGAGGTGCTAGCTATGCTTCAGTGGTTAAGACGACCATTATGTTGGCTGATCTGAAGGATTTCAAGAAAGTAAACGAGATTTATGCTAAATACTTTCTGTCCCCTGCTCCAGCTCGGTCAACATTTCAGGTTGCAGCATTGCCTTTGGATGCTAAGGTTGAAATCGAGTGCATCGCTACACTTTGA

Coding sequence (CDS)

ATGGCTTGGTGTGCGGCTCGGACCTTCCACATGCCGGCGTTCGACGTCACTGCGCTCCGCTCCAAGACCCCGCTAGCCGTCGGCGTCGGTTGCGCTTCCGTCGCCGGCACCACCTTGTGGCGATCTTCATCAACTTCCCCTGCTTCCAAGCGCCAAATGCCGTTCGCATCCCTTGGCATTTCCGCGGATACTTGTATCAAGGAAGCTGTTCAGACTGACAAGGCTCCAGCAGCATTAGGGCCATATTCTCAGGCTATCAAAGCCAACAACCTTCTCTTTGTGTCTGGTGTCCTAGGTCTAAATCCTGAGACAGGGAAATTTGTCTCAGATGATGTTGAAGGCCAAACTGAGCAGGTCCTCAAAAATATGGGTGAAATATTGAAAGCTGGAGGTGCTAGCTATGCTTCAGTGGTTAAGACGACCATTATGTTGGCTGATCTGAAGGATTTCAAGAAAGTAAACGAGATTTATGCTAAATACTTTCTGTCCCCTGCTCCAGCTCGGTCAACATTTCAGGTTGCAGCATTGCCTTTGGATGCTAAGGTTGAAATCGAGTGCATCGCTACACTTTGA

Protein sequence

MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGISADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLKNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDAKVEIECIATL
Homology
BLAST of Moc10g13830 vs. NCBI nr
Match: XP_038903207.1 (reactive Intermediate Deaminase A, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 341.3 bits (874), Expect = 5.5e-90
Identity = 178/190 (93.68%), Postives = 181/190 (95.26%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSST   SKRQMPFASLGI
Sbjct: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSST---SKRQMPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S DT IKEAVQTDKAPAALGPYSQAIKANNLLFVSG LGLNPETGKFVSDDVE QTEQVL
Sbjct: 61  STDTSIKEAVQTDKAPAALGPYSQAIKANNLLFVSGCLGLNPETGKFVSDDVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGASY+SVVKTTIMLADLKDFKKVNEIY KYF SP+PARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGASYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPSPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. NCBI nr
Match: XP_004152932.1 (reactive Intermediate Deaminase A, chloroplastic [Cucumis sativus] >KGN56123.1 hypothetical protein Csa_009669 [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 1.5e-87
Identity = 173/190 (91.05%), Postives = 180/190 (94.74%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAARTFHMPAFDVTALRSK+PLAVGVGC SVAGTTLWRSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCAARTFHMPAFDVTALRSKSPLAVGVGCVSVAGTTLWRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S  + IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVE QTEQVL
Sbjct: 61  STSSSIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGG+SY+SVVKTTIMLADLKDFKKVNEIYAKYF SPAPARST++VA LPLDA
Sbjct: 121 KNMGEILKAGGSSYSSVVKTTIMLADLKDFKKVNEIYAKYFPSPAPARSTYEVARLPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. NCBI nr
Match: XP_008463412.1 (PREDICTED: reactive Intermediate Deaminase A, chloroplastic-like [Cucumis melo])

HSP 1 Score: 332.4 bits (851), Expect = 2.5e-87
Identity = 173/190 (91.05%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCA R+FHMPAFDVTALRSKTPLAVGVGC SVAGTTLWRSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCATRSFHMPAFDVTALRSKTPLAVGVGCVSVAGTTLWRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S  + IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSD+VE QTEQVL
Sbjct: 61  STSSSIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDNVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGASY+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGASYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. NCBI nr
Match: XP_022976324.1 (reactive Intermediate Deaminase A, chloroplastic [Cucurbita maxima])

HSP 1 Score: 330.1 bits (845), Expect = 1.3e-86
Identity = 172/190 (90.53%), Postives = 178/190 (93.68%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAAR  HMPA D+TALRSKTPLAVGVGCASVAGTT  RSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCAARALHMPALDITALRSKTPLAVGVGCASVAGTTFCRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S DT IKEAV+TDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDD+EGQTEQVL
Sbjct: 61  STDTSIKEAVKTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDIEGQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGASY+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGASYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. NCBI nr
Match: XP_022942433.1 (reactive Intermediate Deaminase A, chloroplastic [Cucurbita moschata] >XP_023545861.1 reactive Intermediate Deaminase A, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6600781.1 Reactive Intermediate Deaminase A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 325.9 bits (834), Expect = 2.4e-85
Identity = 170/190 (89.47%), Postives = 176/190 (92.63%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAAR  HMPA D+TALRSKTPLAVGVGCASVAGTTL RSSST   SKRQ+PFASL I
Sbjct: 1   MAWCAARALHMPALDITALRSKTPLAVGVGCASVAGTTLCRSSST---SKRQIPFASLSI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S D  IKEAV+TDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDD+EGQTEQVL
Sbjct: 61  STDASIKEAVKTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDIEGQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGA Y+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGAGYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy Swiss-Prot
Match: Q94JQ4 (Reactive Intermediate Deaminase A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIDA PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-65
Identity = 138/193 (71.50%), Postives = 157/193 (81.35%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDV-TALRS-KTPL-AVGVGCASVAGTTLWRSSSTSPASKRQMPFAS 60
           M W   R+ + P  D+ TALRS +TPL A GVGCA+ AG +L+R SS SP      PFAS
Sbjct: 1   MTWSVFRSINTPTLDLSTALRSTRTPLVAAGVGCATFAGVSLFRMSSRSP------PFAS 60

Query: 61  LGISADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTE 120
           L +SA +  KE V T+KAPAALGPYSQAIKANNL+F+SGVLGL PETGKFVS+ VE QTE
Sbjct: 61  LSVSASSVKKEVVSTEKAPAALGPYSQAIKANNLVFLSGVLGLIPETGKFVSESVEDQTE 120

Query: 121 QVLKNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALP 180
           QVLKNMGEILKA GA Y+SVVKTTIMLADL DFK VNEIYAKYF +P+PARST+QVAALP
Sbjct: 121 QVLKNMGEILKASGADYSSVVKTTIMLADLADFKTVNEIYAKYFPAPSPARSTYQVAALP 180

Query: 181 LDAKVEIECIATL 191
           L+AK+EIECIATL
Sbjct: 181 LNAKIEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy Swiss-Prot
Match: P52758 (2-iminobutanoate/2-iminopropanoate deaminase OS=Homo sapiens OX=9606 GN=RIDA PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 63/123 (51.22%), Postives = 90/123 (73.17%), Query Frame = 0

Query: 66  IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLKNMGE 125
           I+  + T KAP A+GPYSQA+  +  +++SG +G++P +G+ VS  V  + +Q LKNMGE
Sbjct: 5   IRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGE 64

Query: 126 ILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDAKVEIE 185
           ILKA G  + +VVKTT++LAD+ DF  VNEIY +YF S  PAR+ +QVAALP  +++EIE
Sbjct: 65  ILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIE 124

Query: 186 CIA 189
            +A
Sbjct: 125 AVA 127

BLAST of Moc10g13830 vs. ExPASy Swiss-Prot
Match: P52760 (2-iminobutanoate/2-iminopropanoate deaminase OS=Mus musculus OX=10090 GN=Rida PE=1 SV=3)

HSP 1 Score: 136.0 bits (341), Expect = 4.6e-31
Identity = 64/123 (52.03%), Postives = 90/123 (73.17%), Query Frame = 0

Query: 66  IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLKNMGE 125
           I++ + T KAPAA+GPYSQA++ +  +++SG +GL+P +G+ V   V  + +Q LKN+GE
Sbjct: 5   IRKVISTTKAPAAIGPYSQAVQVDRTIYISGQVGLDPSSGQLVPGGVVEEAKQALKNLGE 64

Query: 126 ILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDAKVEIE 185
           ILKA G  + +VVKTT++LAD+ DF  VNEIY  YF    PAR+ +QVAALP  ++VEIE
Sbjct: 65  ILKAAGCDFNNVVKTTVLLADMNDFGTVNEIYKTYFQGSLPARAAYQVAALPRGSRVEIE 124

Query: 186 CIA 189
            IA
Sbjct: 125 AIA 127

BLAST of Moc10g13830 vs. ExPASy Swiss-Prot
Match: Q10121 (RutC family protein C23G10.2 OS=Caenorhabditis elegans OX=6239 GN=C23G10.2 PE=3 SV=3)

HSP 1 Score: 135.2 bits (339), Expect = 7.8e-31
Identity = 66/127 (51.97%), Postives = 90/127 (70.87%), Query Frame = 0

Query: 62  ADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLK 121
           A    ++ + +  AP A+GPYSQA++A N +++SG LGL+P+TG      VE QT Q LK
Sbjct: 36  AQKVTRQIISSANAPGAIGPYSQAVRAGNTIYLSGSLGLDPKTGDLKEGVVE-QTHQSLK 95

Query: 122 NMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDAK 181
           N+GE+LKA GA Y +VVKTT++L ++ DF  VNE+Y +YF SP PAR+ +QVAALP    
Sbjct: 96  NLGEVLKAAGADYGNVVKTTVLLQNIADFAAVNEVYGQYFKSPYPARAAYQVAALPKGGL 155

Query: 182 VEIECIA 189
           VEIE +A
Sbjct: 156 VEIEAVA 161

BLAST of Moc10g13830 vs. ExPASy Swiss-Prot
Match: P52759 (2-iminobutanoate/2-iminopropanoate deaminase OS=Rattus norvegicus OX=10116 GN=Rida PE=1 SV=3)

HSP 1 Score: 132.5 bits (332), Expect = 5.0e-30
Identity = 62/123 (50.41%), Postives = 88/123 (71.54%), Query Frame = 0

Query: 66  IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLKNMGE 125
           I++ + T KAPAA+G YSQA+  +  ++VSG +G++P +G+ V   V  + +Q LKN+GE
Sbjct: 5   IRKVISTSKAPAAIGAYSQAVLVDRTIYVSGQIGMDPSSGQLVPGGVAEEAKQALKNLGE 64

Query: 126 ILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDAKVEIE 185
           ILKA G  + +VVKTT++LAD+ DF  VNEIY  YF    PAR+ +QVAALP  +++EIE
Sbjct: 65  ILKAAGCDFTNVVKTTVLLADINDFGTVNEIYKTYFQGNLPARAAYQVAALPKGSRIEIE 124

Query: 186 CIA 189
            IA
Sbjct: 125 AIA 127

BLAST of Moc10g13830 vs. ExPASy TrEMBL
Match: A0A0A0L2Z6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G077580 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 7.2e-88
Identity = 173/190 (91.05%), Postives = 180/190 (94.74%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAARTFHMPAFDVTALRSK+PLAVGVGC SVAGTTLWRSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCAARTFHMPAFDVTALRSKSPLAVGVGCVSVAGTTLWRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S  + IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVE QTEQVL
Sbjct: 61  STSSSIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGG+SY+SVVKTTIMLADLKDFKKVNEIYAKYF SPAPARST++VA LPLDA
Sbjct: 121 KNMGEILKAGGSSYSSVVKTTIMLADLKDFKKVNEIYAKYFPSPAPARSTYEVARLPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy TrEMBL
Match: A0A1S3CJ54 (reactive Intermediate Deaminase A, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103501583 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.2e-87
Identity = 173/190 (91.05%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCA R+FHMPAFDVTALRSKTPLAVGVGC SVAGTTLWRSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCATRSFHMPAFDVTALRSKTPLAVGVGCVSVAGTTLWRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S  + IKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSD+VE QTEQVL
Sbjct: 61  STSSSIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDNVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGASY+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGASYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy TrEMBL
Match: A0A6J1IN69 (reactive Intermediate Deaminase A, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476753 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.1e-87
Identity = 172/190 (90.53%), Postives = 178/190 (93.68%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAAR  HMPA D+TALRSKTPLAVGVGCASVAGTT  RSSST   SKRQ+PFASLGI
Sbjct: 1   MAWCAARALHMPALDITALRSKTPLAVGVGCASVAGTTFCRSSST---SKRQIPFASLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S DT IKEAV+TDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDD+EGQTEQVL
Sbjct: 61  STDTSIKEAVKTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDIEGQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGASY+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGASYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy TrEMBL
Match: A0A6J1FUT7 (reactive Intermediate Deaminase A, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447476 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.1e-85
Identity = 170/190 (89.47%), Postives = 176/190 (92.63%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAAR  HMPA D+TALRSKTPLAVGVGCASVAGTTL RSSST   SKRQ+PFASL I
Sbjct: 1   MAWCAARALHMPALDITALRSKTPLAVGVGCASVAGTTLCRSSST---SKRQIPFASLSI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S D  IKEAV+TDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDD+EGQTEQVL
Sbjct: 61  STDASIKEAVKTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDIEGQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGA Y+SVVKTTIMLADLKDFKKVNEIY KYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGAGYSSVVKTTIMLADLKDFKKVNEIYGKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           KVEIECIATL
Sbjct: 181 KVEIECIATL 187

BLAST of Moc10g13830 vs. ExPASy TrEMBL
Match: A0A6P4APP4 (reactive Intermediate Deaminase A, chloroplastic OS=Ziziphus jujuba OX=326968 GN=LOC107431842 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.2e-78
Identity = 157/190 (82.63%), Postives = 171/190 (90.00%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDVTALRSKTPLAVGVGCASVAGTTLWRSSSTSPASKRQMPFASLGI 60
           MAWCAARTF++PA DV ALR++ PLAVGVG ASVAG+ +WRSSS   + KR  PFA LGI
Sbjct: 1   MAWCAARTFNVPAIDVGALRTRAPLAVGVGFASVAGSNIWRSSS---SMKRSAPFACLGI 60

Query: 61  SADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVL 120
           S DT IKEAV+TDKAPAALGPYSQAIKANN L+VSGVLGL PETGKFVSD+VE QTEQVL
Sbjct: 61  STDTRIKEAVKTDKAPAALGPYSQAIKANNFLYVSGVLGLIPETGKFVSDNVEDQTEQVL 120

Query: 121 KNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALPLDA 180
           KNMGEILKAGGA Y+SVVKTTIMLADLKDFKKVNEIYAKYF SPAPARST+QVAALPLDA
Sbjct: 121 KNMGEILKAGGAGYSSVVKTTIMLADLKDFKKVNEIYAKYFPSPAPARSTYQVAALPLDA 180

Query: 181 KVEIECIATL 191
           K+EIECIA+L
Sbjct: 181 KIEIECIASL 187

BLAST of Moc10g13830 vs. TAIR 10
Match: AT3G20390.1 (endoribonuclease L-PSP family protein )

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-66
Identity = 138/193 (71.50%), Postives = 157/193 (81.35%), Query Frame = 0

Query: 1   MAWCAARTFHMPAFDV-TALRS-KTPL-AVGVGCASVAGTTLWRSSSTSPASKRQMPFAS 60
           M W   R+ + P  D+ TALRS +TPL A GVGCA+ AG +L+R SS SP      PFAS
Sbjct: 1   MTWSVFRSINTPTLDLSTALRSTRTPLVAAGVGCATFAGVSLFRMSSRSP------PFAS 60

Query: 61  LGISADTCIKEAVQTDKAPAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTE 120
           L +SA +  KE V T+KAPAALGPYSQAIKANNL+F+SGVLGL PETGKFVS+ VE QTE
Sbjct: 61  LSVSASSVKKEVVSTEKAPAALGPYSQAIKANNLVFLSGVLGLIPETGKFVSESVEDQTE 120

Query: 121 QVLKNMGEILKAGGASYASVVKTTIMLADLKDFKKVNEIYAKYFLSPAPARSTFQVAALP 180
           QVLKNMGEILKA GA Y+SVVKTTIMLADL DFK VNEIYAKYF +P+PARST+QVAALP
Sbjct: 121 QVLKNMGEILKASGADYSSVVKTTIMLADLADFKTVNEIYAKYFPAPSPARSTYQVAALP 180

Query: 181 LDAKVEIECIATL 191
           L+AK+EIECIATL
Sbjct: 181 LNAKIEIECIATL 187

BLAST of Moc10g13830 vs. TAIR 10
Match: AT3G04480.1 (endoribonucleases )

HSP 1 Score: 41.6 bits (96), Expect = 8.3e-04
Identity = 26/100 (26.00%), Postives = 51/100 (51.00%), Query Frame = 0

Query: 75  APAALGPYSQAIKANNLLFVSGVLGLNPETGKFVSDDVEGQTEQVLKNMGEILKAGGASY 134
           AP+ +GPYSQA    ++L ++G LGL+P T    ++    +  Q L N   I ++   S 
Sbjct: 424 APSCIGPYSQATLHQSVLHMAGQLGLDPPTMNLQTEGAIAELNQALTNSEAIAESFNCSI 483

Query: 135 ASVVKTTIMLADLKDFK-KVNEIYAKYFLSPAPARSTFQV 174
           +S     ++    +  + + N+++ K+      A+S+ +V
Sbjct: 484 SSSAILFVVFCSARTKQSERNQLHEKFVTFLGLAKSSRRV 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903207.15.5e-9093.68reactive Intermediate Deaminase A, chloroplastic-like [Benincasa hispida][more]
XP_004152932.11.5e-8791.05reactive Intermediate Deaminase A, chloroplastic [Cucumis sativus] >KGN56123.1 h... [more]
XP_008463412.12.5e-8791.05PREDICTED: reactive Intermediate Deaminase A, chloroplastic-like [Cucumis melo][more]
XP_022976324.11.3e-8690.53reactive Intermediate Deaminase A, chloroplastic [Cucurbita maxima][more]
XP_022942433.12.4e-8589.47reactive Intermediate Deaminase A, chloroplastic [Cucurbita moschata] >XP_023545... [more]
Match NameE-valueIdentityDescription
Q94JQ42.8e-6571.50Reactive Intermediate Deaminase A, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
P527581.2e-3151.222-iminobutanoate/2-iminopropanoate deaminase OS=Homo sapiens OX=9606 GN=RIDA PE=... [more]
P527604.6e-3152.032-iminobutanoate/2-iminopropanoate deaminase OS=Mus musculus OX=10090 GN=Rida PE... [more]
Q101217.8e-3151.97RutC family protein C23G10.2 OS=Caenorhabditis elegans OX=6239 GN=C23G10.2 PE=3 ... [more]
P527595.0e-3050.412-iminobutanoate/2-iminopropanoate deaminase OS=Rattus norvegicus OX=10116 GN=Ri... [more]
Match NameE-valueIdentityDescription
A0A0A0L2Z67.2e-8891.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G077580 PE=3 SV=1[more]
A0A1S3CJ541.2e-8791.05reactive Intermediate Deaminase A, chloroplastic-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1IN696.1e-8790.53reactive Intermediate Deaminase A, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1FUT71.1e-8589.47reactive Intermediate Deaminase A, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A6P4APP45.2e-7882.63reactive Intermediate Deaminase A, chloroplastic OS=Ziziphus jujuba OX=326968 GN... [more]
Match NameE-valueIdentityDescription
AT3G20390.12.0e-6671.50endoribonuclease L-PSP family protein [more]
AT3G04480.18.3e-0426.00endoribonucleases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006175YjgF/YER057c/UK114 familyPFAMPF01042Ribonuc_L-PSPcoord: 73..189
e-value: 1.7E-41
score: 141.0
IPR006175YjgF/YER057c/UK114 familyPANTHERPTHR118032-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDAcoord: 1..190
IPR006056RidA familyTIGRFAMTIGR00004TIGR00004coord: 67..189
e-value: 9.2E-51
score: 169.2
IPR035959RutC-like superfamilyGENE3D3.30.1330.40coord: 62..189
e-value: 1.5E-48
score: 166.1
IPR035959RutC-like superfamilySUPERFAMILY55298YjgF-likecoord: 67..190
NoneNo IPR availablePANTHERPTHR11803:SF45PERCHLORIC ACID SOLUBLE TRANSLATION INHIBITOR-LIKE PROTEINcoord: 1..190
NoneNo IPR availableCDDcd00448YjgF_YER057c_UK114_familycoord: 82..188
e-value: 6.04757E-44
score: 140.001
IPR019897RidA, conserved sitePROSITEPS01094UPF0076coord: 166..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g13830.1Moc10g13830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901565 organonitrogen compound catabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0019239 deaminase activity