Moc09g06210 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g06210
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionstachyose synthase-like
Locationchr9: 4831477 .. 4834167 (-)
RNA-Seq ExpressionMoc09g06210
SyntenyMoc09g06210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGATTGGAAATTCTGGATCAGATTTACAAATGGAAACTCAATGGGTCATCTTAAATGTGCCTGAAGTAAAGTCATATGTCATTTTCATACCCATCATAGAAGGCAGTTTCAGATCTGCCCTTCATCCTGGGGATGATGGGCAGGTGTTTATTTGTGTTGAAAGTGGCTCAACTCATGTGAAAGCATCAAGTTTTGATGCAATTGCTTACGTTCATGTGTCTGATAACCCCTACAATTTAATGAAAGAGGCCTATGCTGCCATTCGAGTCCATTTGAATACTTTTAGACTCTTGGAAGGGAAGCCTGTCACACATCTGGTGGATAAATTCGGTTGGTGCACTTGGGATGCTTTCTACTTAACAGTAGACCCTATTGGAATTTGGAATGGTCTTAATGATTTTGCTAAAGAAGGCATCTCGCCGAGGTTCCTCATCATCGACGATGGGTGGCAAAGCATCAATATGGATGGCGACGACCCGAATCGAGATGCTAAAAATCTTGTTCTATGTGGGACTCAGATGACTGCCAGGCTCTACAGATTTGAAGAGGCCGAAAAATTCAGAAAGTACAAAGGAGGGTCTTTGTTGGGTCCAAATGCTCCATCTTTCAATCCAAAGAGGCCGAAGTTGCTTATCGCGAAGGCGATCGAGATCGAGCATGCTGAGAAAGACAGAGACAAGGCTATTGCATCTGGAGTCAATGAAATATCTAAGTTTGAAATAAAAATTCAGAAGCTGAAGGAAGAGTTGGATGATATTTTTGGGAAAGAAGATGAAGAAAGTGGTGCTGTAATCAAAGGTTGTTCAAGCTGTTCTTGCAAGGCTGATGACTCCGGAATGAAGGCTTTCGTGAGGGACTTGAAGACTAAATTCAAAGGTTTGGATGATGTATTTGTTTGGCATGCTCTTGCTGGTGCTTGGGGCGGTGTAAGGCCAGGGGCTACGCATCTAAGTTCCAATTTAACTCCCTGCAAGCTCTCTCCCGGGCTCGGAGACACGATGGACGATCTCGCTGTTGTGAAGATCTTCGATGGTAGCATCGGACTTGTTCATCCCAATCAAGCTGACGATTTCTTTGATTCCATGCATTCCTATCTTTCTAAAGTTGGGATTGCAGGAGTGAAAGTTGATGTGATAAACGTAAGTTTCTCTAAAACAAAACCTTCAGTTTGCTTTTGACATAGTGCAATGAAGAACTAATCTATCAACCATTTAGACTCTAGAGTATGTCTCAGAGGAACATGGAGGACGAGTTGATCTTGCAAAGGCCTATTATAAGGGTCTGACCAACTCTATTCTCAAGAACTTCAAAGGGACTGGCCTTTTCTCTAGCATGCAACAATGTAATGATTTCTTCTTCCTTGGCACAAAGCAAAACTCCATAGGAAGAGTTGGTAAGAACCTCAACTTTTTCCTCTCTCTCTCAAACTCCATGGGAAGAGTTGGTAAGAATTTCAGCTTTTTCCTCTCGCTGATCTCTGGTCACTTGCTTGAAGTTTACAAAAGGGATGACCAAAATGAAACAAAAGAACAGAAAAAGGTAAACATTTGGATTAAAACTTAACTATAACTTATGTTTTGGGGGACAGGTGAAGATTTTTGGTTCCAAGATCCAAATGGTGACCCCATGGGTATTTACTGGTTACAAGGAGTTCATATGATCCACTGTGCATACAACAGCATGTGGATCGGACAGATCATCCAGCCTGATTGGGACATGTTCCAATCAGACCATCTATGTGCCAAGTTCCATGCAGGATCAAGAGCCATTTGTGGGGGTCCTGTGTATGTGAGTGACTCCGTGGGTGGCCATAATTTTGATCTTAAAAAGCAGCTTGTGTATCCAGATGGAACTATTCCTAGGTGCCAGCATTTTGCCCTACCCACTAGAGATTGCCTCTTCAAAAATACCCTATTTGACGGCAGAACTGTTCTCAAGATTTGGAACCTTAACAAGGTATATAAATATACATATATATATTATACTAAAAGTTCTAAGAAGAAAGTAAATGCAGTTCTAAAAGTTCATGAACCAAATTCATAATTTAACCTTCTTTATCTGTGAGTCTTTGTATTCTACCAAAATATGACATTATTTTCATCTTCTTCGGAATATAATTACAGTATGGAGGAGTAATTGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCAGAAGAGCGAAGGATTAAGGGGCATCCAGAGTGTTACAAGCCAATGTCCATGACAATACATGTTAGTGATGTGGAATGGGACCAAAAACCAGAAGCAGCCCCAATGGGGAATTTCGTCGAATACATTGTGTACCTGAACCAAGCCGAGCAAATTCTCCACACGACCCCAAAATCCGAGCCGCTAGAAGTAACCGTTCAACCATCCACATTCGAGCTCTTCAGCTTCATACCCCTCCGAAAGCTCGGCTCCAGCATCAAATTCGCCCCCATCGGCCTCACGAACATGTTCAACAGCTCCGGCACAATCCAACACTTGAAGTATAATGAAAATGGAGTGGAGCTGAAGGTGAAAGGAGGAGGAAGCTTCTTGGCCTACTCGAGCGGATCGCCGAAGCAGTGCCTTTCGAACGGAATGGAGGTGGAATTCGAATGGGATTCCGACGGAAAGCTAGGGCTTGATCTTCCATGGAATGGAGAAGCCGACGGAGTTTCTAATTTAGATATTTTCTTCTGA

mRNA sequence

ATGTGGATTGGAAATTCTGGATCAGATTTACAAATGGAAACTCAATGGGTCATCTTAAATGTGCCTGAAGTAAAGTCATATGTCATTTTCATACCCATCATAGAAGGCAGTTTCAGATCTGCCCTTCATCCTGGGGATGATGGGCAGGTGTTTATTTGTGTTGAAAGTGGCTCAACTCATGTGAAAGCATCAAGTTTTGATGCAATTGCTTACGTTCATGTGTCTGATAACCCCTACAATTTAATGAAAGAGGCCTATGCTGCCATTCGAGTCCATTTGAATACTTTTAGACTCTTGGAAGGGAAGCCTGTCACACATCTGGTGGATAAATTCGGTTGGTGCACTTGGGATGCTTTCTACTTAACAGTAGACCCTATTGGAATTTGGAATGGTCTTAATGATTTTGCTAAAGAAGGCATCTCGCCGAGGTTCCTCATCATCGACGATGGGTGGCAAAGCATCAATATGGATGGCGACGACCCGAATCGAGATGCTAAAAATCTTGTTCTATGTGGGACTCAGATGACTGCCAGGCTCTACAGATTTGAAGAGGCCGAAAAATTCAGAAAGTACAAAGGAGGGTCTTTGTTGGGTCCAAATGCTCCATCTTTCAATCCAAAGAGGCCGAAGTTGCTTATCGCGAAGGCGATCGAGATCGAGCATGCTGAGAAAGACAGAGACAAGGCTATTGCATCTGGAGTCAATGAAATATCTAAGTTTGAAATAAAAATTCAGAAGCTGAAGGAAGAGTTGGATGATATTTTTGGGAAAGAAGATGAAGAAAGTGGTGCTGTAATCAAAGGTTGTTCAAGCTGTTCTTGCAAGGCTGATGACTCCGGAATGAAGGCTTTCGTGAGGGACTTGAAGACTAAATTCAAAGGTTTGGATGATGTATTTGTTTGGCATGCTCTTGCTGGTGCTTGGGGCGGTGTAAGGCCAGGGGCTACGCATCTAAGTTCCAATTTAACTCCCTGCAAGCTCTCTCCCGGGCTCGGAGACACGATGGACGATCTCGCTGTTGTGAAGATCTTCGATGGTAGCATCGGACTTGTTCATCCCAATCAAGCTGACGATTTCTTTGATTCCATGCATTCCTATCTTTCTAAAGTTGGGATTGCAGGAGTGAAAGTTGATGTGATAAACACTCTAGAGTATGTCTCAGAGGAACATGGAGGACGAGTTGATCTTGCAAAGGCCTATTATAAGGGTCTGACCAACTCTATTCTCAAGAACTTCAAAGGGACTGGCCTTTTCTCTAGCATGCAACAATGTAATGATTTCTTCTTCCTTGGCACAAAGCAAAACTCCATAGGAAGAGTTGGTGAAGATTTTTGGTTCCAAGATCCAAATGGTGACCCCATGGGTATTTACTGGTTACAAGGAGTTCATATGATCCACTGTGCATACAACAGCATGTGGATCGGACAGATCATCCAGCCTGATTGGGACATGTTCCAATCAGACCATCTATGTGCCAAGTTCCATGCAGGATCAAGAGCCATTTGTGGGGGTCCTGTGTATGTGAGTGACTCCGTGGGTGGCCATAATTTTGATCTTAAAAAGCAGCTTGTGTATCCAGATGGAACTATTCCTAGGTGCCAGCATTTTGCCCTACCCACTAGAGATTGCCTCTTCAAAAATACCCTATTTGACGGCAGAACTGTTCTCAAGATTTGGAACCTTAACAAGTATGGAGGAGTAATTGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCAGAAGAGCGAAGGATTAAGGGGCATCCAGAGTGTTACAAGCCAATGTCCATGACAATACATGTTAGTGATGTGGAATGGGACCAAAAACCAGAAGCAGCCCCAATGGGGAATTTCGTCGAATACATTGTGTACCTGAACCAAGCCGAGCAAATTCTCCACACGACCCCAAAATCCGAGCCGCTAGAAGTAACCGTTCAACCATCCACATTCGAGCTCTTCAGCTTCATACCCCTCCGAAAGCTCGGCTCCAGCATCAAATTCGCCCCCATCGGCCTCACGAACATGTTCAACAGCTCCGGCACAATCCAACACTTGAAGTATAATGAAAATGGAGTGGAGCTGAAGGTGAAAGGAGGAGGAAGCTTCTTGGCCTACTCGAGCGGATCGCCGAAGCAGTGCCTTTCGAACGGAATGGAGGTGGAATTCGAATGGGATTCCGACGGAAAGCTAGGGCTTGATCTTCCATGGAATGGAGAAGCCGACGGAGTTTCTAATTTAGATATTTTCTTCTGA

Coding sequence (CDS)

ATGTGGATTGGAAATTCTGGATCAGATTTACAAATGGAAACTCAATGGGTCATCTTAAATGTGCCTGAAGTAAAGTCATATGTCATTTTCATACCCATCATAGAAGGCAGTTTCAGATCTGCCCTTCATCCTGGGGATGATGGGCAGGTGTTTATTTGTGTTGAAAGTGGCTCAACTCATGTGAAAGCATCAAGTTTTGATGCAATTGCTTACGTTCATGTGTCTGATAACCCCTACAATTTAATGAAAGAGGCCTATGCTGCCATTCGAGTCCATTTGAATACTTTTAGACTCTTGGAAGGGAAGCCTGTCACACATCTGGTGGATAAATTCGGTTGGTGCACTTGGGATGCTTTCTACTTAACAGTAGACCCTATTGGAATTTGGAATGGTCTTAATGATTTTGCTAAAGAAGGCATCTCGCCGAGGTTCCTCATCATCGACGATGGGTGGCAAAGCATCAATATGGATGGCGACGACCCGAATCGAGATGCTAAAAATCTTGTTCTATGTGGGACTCAGATGACTGCCAGGCTCTACAGATTTGAAGAGGCCGAAAAATTCAGAAAGTACAAAGGAGGGTCTTTGTTGGGTCCAAATGCTCCATCTTTCAATCCAAAGAGGCCGAAGTTGCTTATCGCGAAGGCGATCGAGATCGAGCATGCTGAGAAAGACAGAGACAAGGCTATTGCATCTGGAGTCAATGAAATATCTAAGTTTGAAATAAAAATTCAGAAGCTGAAGGAAGAGTTGGATGATATTTTTGGGAAAGAAGATGAAGAAAGTGGTGCTGTAATCAAAGGTTGTTCAAGCTGTTCTTGCAAGGCTGATGACTCCGGAATGAAGGCTTTCGTGAGGGACTTGAAGACTAAATTCAAAGGTTTGGATGATGTATTTGTTTGGCATGCTCTTGCTGGTGCTTGGGGCGGTGTAAGGCCAGGGGCTACGCATCTAAGTTCCAATTTAACTCCCTGCAAGCTCTCTCCCGGGCTCGGAGACACGATGGACGATCTCGCTGTTGTGAAGATCTTCGATGGTAGCATCGGACTTGTTCATCCCAATCAAGCTGACGATTTCTTTGATTCCATGCATTCCTATCTTTCTAAAGTTGGGATTGCAGGAGTGAAAGTTGATGTGATAAACACTCTAGAGTATGTCTCAGAGGAACATGGAGGACGAGTTGATCTTGCAAAGGCCTATTATAAGGGTCTGACCAACTCTATTCTCAAGAACTTCAAAGGGACTGGCCTTTTCTCTAGCATGCAACAATGTAATGATTTCTTCTTCCTTGGCACAAAGCAAAACTCCATAGGAAGAGTTGGTGAAGATTTTTGGTTCCAAGATCCAAATGGTGACCCCATGGGTATTTACTGGTTACAAGGAGTTCATATGATCCACTGTGCATACAACAGCATGTGGATCGGACAGATCATCCAGCCTGATTGGGACATGTTCCAATCAGACCATCTATGTGCCAAGTTCCATGCAGGATCAAGAGCCATTTGTGGGGGTCCTGTGTATGTGAGTGACTCCGTGGGTGGCCATAATTTTGATCTTAAAAAGCAGCTTGTGTATCCAGATGGAACTATTCCTAGGTGCCAGCATTTTGCCCTACCCACTAGAGATTGCCTCTTCAAAAATACCCTATTTGACGGCAGAACTGTTCTCAAGATTTGGAACCTTAACAAGTATGGAGGAGTAATTGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCAGAAGAGCGAAGGATTAAGGGGCATCCAGAGTGTTACAAGCCAATGTCCATGACAATACATGTTAGTGATGTGGAATGGGACCAAAAACCAGAAGCAGCCCCAATGGGGAATTTCGTCGAATACATTGTGTACCTGAACCAAGCCGAGCAAATTCTCCACACGACCCCAAAATCCGAGCCGCTAGAAGTAACCGTTCAACCATCCACATTCGAGCTCTTCAGCTTCATACCCCTCCGAAAGCTCGGCTCCAGCATCAAATTCGCCCCCATCGGCCTCACGAACATGTTCAACAGCTCCGGCACAATCCAACACTTGAAGTATAATGAAAATGGAGTGGAGCTGAAGGTGAAAGGAGGAGGAAGCTTCTTGGCCTACTCGAGCGGATCGCCGAAGCAGTGCCTTTCGAACGGAATGGAGGTGGAATTCGAATGGGATTCCGACGGAAAGCTAGGGCTTGATCTTCCATGGAATGGAGAAGCCGACGGAGTTTCTAATTTAGATATTTTCTTCTGA

Protein sequence

MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFVWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRKLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEVEFEWDSDGKLGLDLPWNGEADGVSNLDIFF
Homology
BLAST of Moc09g06210 vs. NCBI nr
Match: XP_022148001.1 (stachyose synthase-like [Momordica charantia])

HSP 1 Score: 1467.6 bits (3798), Expect = 0.0e+00
Identity = 685/750 (91.33%), Postives = 721/750 (96.13%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MWIGNSGSDLQMETQWVILNVPE+KSYV+FIPIIEGSFRSALHPG DGQV IC ESGSTH
Sbjct: 114 MWIGNSGSDLQMETQWVILNVPEIKSYVVFIPIIEGSFRSALHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAAIRVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKVSSFDAIAYVHVSDNPYKLMKEAYAAIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDPIGIWNG+NDFA+ GISPRFLIIDDGWQSINMD +DPNRD KNL+L GTQMT+RLY
Sbjct: 234 LTVDPIGIWNGVNDFAEGGISPRFLIIDDGWQSINMDDEDPNRDGKNLILGGTQMTSRLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RFEE EKFRKYKGGSLLGPNAPSF+PK+PKLLIAKAIE+EHAE+DRD+AIASGVN+ SKF
Sbjct: 294 RFEECEKFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIELEHAERDRDEAIASGVNDTSKF 353

Query: 241 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 300
           EIKIQKLKEE+D+IFGKEDEESGAV KGCSSCSCKAD+SGMKAF RDLKTKFKGLDD+FV
Sbjct: 354 EIKIQKLKEEVDEIFGKEDEESGAVTKGCSSCSCKADNSGMKAFTRDLKTKFKGLDDIFV 413

Query: 301 WHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFF 360
           WHALAGAWGGVRPGATHLSS + PCKLSPGL  TM DLAVVKI +GSIGLVHPNQADDFF
Sbjct: 414 WHALAGAWGGVRPGATHLSSKIVPCKLSPGLDGTMTDLAVVKIVEGSIGLVHPNQADDFF 473

Query: 361 DSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSS 420
           DSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFSS
Sbjct: 474 DSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSS 533

Query: 421 MQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQP 480
           MQQCNDFFFLGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQP
Sbjct: 534 MQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQP 593

Query: 481 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPT 540
           DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALPT
Sbjct: 594 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPT 653

Query: 541 RDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIH 600
           RDCLFKN+LFDG+TVLKIWNLNKYGGVIGAFNCQGAGWDP+E+RIKGHPECYKP S T+H
Sbjct: 654 RDCLFKNSLFDGKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPTSTTVH 713

Query: 601 VSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRK 660
           VSDVEWDQK EAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+VT+QPSTFELFSFIPLRK
Sbjct: 714 VSDVEWDQKQEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRK 773

Query: 661 LGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEV 720
           LGSSIKFAPIGLTNMFNSSGTIQHLKYNE GVELKVKGGGSFLAYS+ SPK+C+SNGMEV
Sbjct: 774 LGSSIKFAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGGGSFLAYSNASPKKCVSNGMEV 833

Query: 721 EFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           EFEWDSDGKLG DLPWNGEA GVSNLDIFF
Sbjct: 834 EFEWDSDGKLGFDLPWNGEAGGVSNLDIFF 863

BLAST of Moc09g06210 vs. NCBI nr
Match: XP_022148013.1 (stachyose synthase-like [Momordica charantia])

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 674/750 (89.87%), Postives = 717/750 (95.60%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSD+QMETQWV+LNVPE+KSYV+FIPIIEGSFRSAL+PG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDIQMETQWVMLNVPEIKSYVVFIPIIEGSFRSALYPGADGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVS+NPYNLMKEAYAAIRVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKESSFDAIAYVHVSNNPYNLMKEAYAAIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP GIWNGLNDFA+ GISPRFLIIDDGWQSINMD +DPN+D K+LVLCGTQMT+RLY
Sbjct: 234 LTVDPTGIWNGLNDFAEGGISPRFLIIDDGWQSINMDDEDPNQDGKHLVLCGTQMTSRLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           +FEE EKF+KYKGGS LGPN PSFNPKRPKLLI KA EIE A++DRD+AIA GVN++SKF
Sbjct: 294 KFEECEKFKKYKGGSFLGPNVPSFNPKRPKLLIQKADEIEDAKRDRDEAIAYGVNDVSKF 353

Query: 241 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 300
           EIKIQKLKEELDDIFGKEDEESGAV KGCS+ SCKA DSGMKAF RDLKTKFKGLDDVFV
Sbjct: 354 EIKIQKLKEELDDIFGKEDEESGAVTKGCSTGSCKAADSGMKAFTRDLKTKFKGLDDVFV 413

Query: 301 WHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFF 360
           WHALAGAWGGVRPGATHL+SNL PCKLSPGL  TMDDLAVVKI +GSIGLVHPNQADDFF
Sbjct: 414 WHALAGAWGGVRPGATHLNSNLIPCKLSPGLDGTMDDLAVVKIIEGSIGLVHPNQADDFF 473

Query: 361 DSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSS 420
           DSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS++KNFKGTGLFSS
Sbjct: 474 DSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLIKNFKGTGLFSS 533

Query: 421 MQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQP 480
           MQQCNDFFFLGTKQNSIGRVG+DFWFQDPNGDPMGIYWLQGVHMIHCAYNSMW+GQIIQP
Sbjct: 534 MQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWMGQIIQP 593

Query: 481 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPT 540
           DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALPT
Sbjct: 594 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPT 653

Query: 541 RDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIH 600
           RDC+FKN+LFDG+TVLKIWNLNKYGGVIGAFNCQGAGWDP+ERRIKGHP+CYKPMS TIH
Sbjct: 654 RDCIFKNSLFDGKTVLKIWNLNKYGGVIGAFNCQGAGWDPKERRIKGHPKCYKPMSTTIH 713

Query: 601 VSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRK 660
           VSDVEWDQKPEAAPMGNFVEYIVYL+QAEQILHTT KSEPL+ ++QPSTFELFSFIPLRK
Sbjct: 714 VSDVEWDQKPEAAPMGNFVEYIVYLSQAEQILHTTTKSEPLKASLQPSTFELFSFIPLRK 773

Query: 661 LGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEV 720
           LGSSIKFAPIGLTNMFNSSGTIQHLKYNE GVELKVKGGGSFLAYS+ SPK+C+SNGMEV
Sbjct: 774 LGSSIKFAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGGGSFLAYSNVSPKKCVSNGMEV 833

Query: 721 EFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           EFEW+SDGKLGLDLPWNGEA GVS+LDI F
Sbjct: 834 EFEWNSDGKLGLDLPWNGEAGGVSHLDIIF 863

BLAST of Moc09g06210 vs. NCBI nr
Match: XP_038897999.1 (stachyose synthase [Benincasa hispida])

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 659/751 (87.75%), Postives = 711/751 (94.67%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSALHPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VKASSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKASSFDAIAYVHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG+NDF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GP+APSF+PK+PKLLIAKAIEIEHAEKDRDKAI SGV +ISKF
Sbjct: 294 RFDECEKFRKYKGGSLSGPSAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDISKF 353

Query: 241 EIKIQKLKEELDDIFGKEDEE-SGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KI+KLKEEL +IFGKE+EE S A+ KGC+SCSCKA++SGMKAF RDL+T+FKGLDD+F
Sbjct: 354 ETKIKKLKEELHEIFGKEEEEDSTAISKGCTSCSCKAENSGMKAFTRDLRTQFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPG+THL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGSTHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKG+PECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGYPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGG FLAYSSGSPK+CLSNG+E
Sbjct: 774 KLGSNIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGDFLAYSSGSPKKCLSNGIE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           VEFEWDSDGKL  DLPW  E  GVSNLDIFF
Sbjct: 834 VEFEWDSDGKLSFDLPWIEETGGVSNLDIFF 864

BLAST of Moc09g06210 vs. NCBI nr
Match: NP_001267675.1 (steryl-sulfatase [Cucumis sativus] >ABQ53598.1 stachyose synthase [Cucumis sativus])

HSP 1 Score: 1418.3 bits (3670), Expect = 0.0e+00
Identity = 657/751 (87.48%), Postives = 707/751 (94.14%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSA+HPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKTSSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG++DF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GPNAPSF+PK+PKLLIAKAIEIEHAEK+RDKAI SGV  +SKF
Sbjct: 294 RFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKF 353

Query: 241 EIKIQKLKEELDDIFGK-EDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KIQKLKEEL  IFGK E+EES A+ KGC+SCSCKAD+SGMKAF RDL+TKFKGLDD+F
Sbjct: 354 ETKIQKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPGATHL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKGHPECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFN SGTIQHLKYNENGVELKVKGGG+FLAYSSGSPK+C+SNG+E
Sbjct: 774 KLGSNIKFAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           VEFEW SDGKL  DL W  EA GVSNLDIFF
Sbjct: 834 VEFEWKSDGKLSFDLHWIEEAGGVSNLDIFF 864

BLAST of Moc09g06210 vs. NCBI nr
Match: ABV44498.1 (stachyose synthetase variant 1 [Cucumis sativus] >ABV44499.1 stachyose synthetase variant 2 [Cucumis sativus] >ABV44500.1 stachyose synthetase variant 3 [Cucumis sativus])

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 656/751 (87.35%), Postives = 706/751 (94.01%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSA+HPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKTSSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG++DF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GPNAPSF+PK+PKLLIAKAIEIEHAEK+RDKAI SGV  +SKF
Sbjct: 294 RFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKF 353

Query: 241 EIKIQKLKEELDDIFGK-EDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KIQKLKEEL  IFGK E+EES A+ KGC+SCSCKAD+SGMKAF RDL+TKFKGLDD+F
Sbjct: 354 ETKIQKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPGATHL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKGHPECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFN SGTIQHLKYNENGVELKVKGGG+FLAYSSGSPK+C+SNG+E
Sbjct: 774 KLGSNIKFAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           VEFEW SDGKL  DL W  EA GV NLDIFF
Sbjct: 834 VEFEWKSDGKLSFDLHWIEEAGGVFNLDIFF 864

BLAST of Moc09g06210 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 501/749 (66.89%), Postives = 612/749 (81.71%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTHV 61
           WIG SGSDLQMETQW+++ VPE KSYV+ IPIIE  FRSAL PG +  V I  ESGST V
Sbjct: 112 WIGKSGSDLQMETQWILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKV 171

Query: 62  KASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFYL 121
           K S+F++IAYVH S+NPY+LMKEAY+AIRVHLN+FRLLE K + +LVDKFGWCTWDAFYL
Sbjct: 172 KESTFNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYL 231

Query: 122 TVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLYR 181
           TV+PIGI++GL+DF+K G+ PRF+IIDDGWQSI+ DG DPN DAKNLVL G QM+ RL+R
Sbjct: 232 TVNPIGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHR 291

Query: 182 FEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKFE 241
           F+E  KFRKY+ G LLGPN+P ++P     LI K IE E   K R++AI+S  +++++ E
Sbjct: 292 FDECYKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIE 351

Query: 242 IKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFVW 301
            KI+K+ +E+DD+FG E   SG         S    + G+KAF +DL+TKFKGLDDV+VW
Sbjct: 352 SKIKKVVKEIDDLFGGEQFSSG-------EKSEMKSEYGLKAFTKDLRTKFKGLDDVYVW 411

Query: 302 HALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFFD 361
           HAL GAWGGVRP  THL + + PCKLSPGL  TM+DLAVV+I   S+GLVHP+QA++ +D
Sbjct: 412 HALCGAWGGVRPETTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYD 471

Query: 362 SMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSSM 421
           SMHSYL++ GI GVKVDVI++LEYV +E+GGRVDLAK YY+GLT SI+KNF G G+ +SM
Sbjct: 472 SMHSYLAESGITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASM 531

Query: 422 QQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQPD 481
           Q CNDFFFLGTKQ S+GRVG+DFWFQDPNGDPMG +WLQGVHMIHC+YNS+W+GQ+IQPD
Sbjct: 532 QHCNDFFFLGTKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPD 591

Query: 482 WDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPTR 541
           WDMFQSDH+CAKFHAGSRAICGGP+YVSD+VG H+FDL K+LV+PDGTIP+C +F LPTR
Sbjct: 592 WDMFQSDHVCAKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTR 651

Query: 542 DCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIHV 601
           DCLFKN LFD  TVLKIWN NKYGGVIGAFNCQGAGWDP  ++ +G PECYKP+  T+HV
Sbjct: 652 DCLFKNPLFDHTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHV 711

Query: 602 SDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRKL 661
           ++VEWDQK E + +G   EY+VYLNQAE++   T KSEP++ T+QPSTFEL+SF+P+ KL
Sbjct: 712 TEVEWDQKEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKL 771

Query: 662 GSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEVE 721
              IKFAPIGLTNMFNS GT+  L+Y  NG ++KVKGGGSFLAYSS SPK+   NG EV+
Sbjct: 772 CGGIKFAPIGLTNMFNSGGTVIDLEYVGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVD 831

Query: 722 FEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           FEW  DGKL +++PW  EA GVS+++IFF
Sbjct: 832 FEWLGDGKLCVNVPWIEEACGVSDMEIFF 853

BLAST of Moc09g06210 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 944.5 bits (2440), Expect = 7.3e-274
Identity = 447/760 (58.82%), Postives = 571/760 (75.13%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTHV 61
           WIG SGSDLQ ETQWV+L +PE+ SYV  IP IEG+FR++L PG+ G V IC ESGST V
Sbjct: 126 WIGKSGSDLQAETQWVMLKIPEIDSYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKV 185

Query: 62  KASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFYL 121
           K SSF +IAY+H+ DNPYNLMKEA++A+RVH+NTF+LLE K +  +VDKFGWCTWDA YL
Sbjct: 186 KESSFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYL 245

Query: 122 TVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLYR 181
           TVDP  IW G+ +F   G+ P+F+IIDDGWQSIN DGD+ ++DA+NLVL G QMTARL  
Sbjct: 246 TVDPATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTS 305

Query: 182 FEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAI-ASGVNEISKF 241
           F+E +KFR YKGGS +  +A  FNP +PK+LI KA E   A   R K +  SG  ++++ 
Sbjct: 306 FKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTEL 365

Query: 242 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 301
           + KI+ L EEL+ +F + ++E        S  S     SGM AF +DL+ +FK LDD++V
Sbjct: 366 DEKIKILSEELNAMFDEVEKEE-------SLGSDDVSGSGMAAFTKDLRLRFKSLDDIYV 425

Query: 302 WHALAGAWGGVRPGA-THLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 361
           WHAL GAW GVRP     L + + P +LSP LG TM DLAV K+ +  IGLVHP++A +F
Sbjct: 426 WHALCGAWNGVRPETMMDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEF 485

Query: 362 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 421
           +DSMHSYL+ VG+ G K+DV  TLE ++EEHGGRV+LAKAYY GLT S++KNF GT + +
Sbjct: 486 YDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIA 545

Query: 422 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 481
           SMQQCN+FFFL TKQ SIGRVG+DFW+QDP GDP G+YWLQGVHMIHC+YNS+W+GQ+IQ
Sbjct: 546 SMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQ 605

Query: 482 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVG--GHNFDLKKQLVYPDGTIPRCQHFA 541
           PDWDMFQSDH+CA++HA SRAICGGPVY+SD +G   HNFDL K+L + DGTIPRC H+A
Sbjct: 606 PDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYA 665

Query: 542 LPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSM 601
           LPTRD LFKN LFD  ++LKI+N NK+GGVIG FNCQGAGW PEE R KG+ ECY  +S 
Sbjct: 666 LPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSG 725

Query: 602 TIHVSDVEWDQKPEAAPMGNFV----EYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELF 661
           T+HVSD+EWDQ PEAA  G+ V    +Y+VY  Q+E+IL    KSE +++T++PS F+L 
Sbjct: 726 TVHVSDIEWDQNPEAA--GSQVTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLL 785

Query: 662 SFIPLRKLGSS-IKFAPIGLTNMFNSSGTIQHLKY-NENGVELKVKGGGSFLAYSSGSPK 721
           SF+P+ +L SS ++FAP+GL NMFN  GT+Q +K   +N + + VKG G F+AYSS +P 
Sbjct: 786 SFVPVTELVSSGVRFAPLGLINMFNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPV 845

Query: 722 QCLSNGMEVEFEWDSD-GKLGLDLPWNGEADGVSNLDIFF 751
           +C  N  E EF+W+ + GKL   +PW  E+ G+S+L   F
Sbjct: 846 KCYLNDKEAEFKWEEETGKLSFFVPWVEESGGISHLSFTF 876

BLAST of Moc09g06210 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.2e-199
Identity = 348/760 (45.79%), Postives = 471/760 (61.97%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKS---------YVIFIPIIEGSFRSALHPGDDGQVFI 61
           W+G++G D++ ETQ +IL+     S         YV+ +P++EGSFRS+   G+D  V +
Sbjct: 106 WVGSNGRDIENETQIIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAV 165

Query: 62  CVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFG 121
           CVESGST V  S F  I YVH  D+P+ L+K+A   IRVH+NTF+LLE K    +VDKFG
Sbjct: 166 CVESGSTEVTGSEFRQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFG 225

Query: 122 WCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCG 181
           WCTWDAFYLTV+P G+  G+      G  P  ++IDDGWQSI  D D  + +  N+ + G
Sbjct: 226 WCTWDAFYLTVNPDGVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAG 285

Query: 182 TQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIAS 241
            QM  RL +FEE  KF+ Y             +PK                         
Sbjct: 286 EQMPCRLLKFEENHKFKDY------------VSPK------------------------- 345

Query: 242 GVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKF 301
                                                       +D GMKAFVRDLK +F
Sbjct: 346 ------------------------------------------DQNDVGMKAFVRDLKDEF 405

Query: 302 KGLDDVFVWHALAGAWGGVRPGATHL-SSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLV 361
             +D ++VWHAL G WGG+RP A  L  S +   +LSPGL  TM+DLAV KI +  IG  
Sbjct: 406 STVDYIYVWHALCGYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFA 465

Query: 362 HPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKN 421
            P+ A +F++ +HS+L   GI GVKVDVI+ LE + +++GGRVDLAKAY+K LT+S+ K+
Sbjct: 466 SPDLAKEFYEGLHSHLQNAGIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKH 525

Query: 422 FKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNS 481
           F G G+ +SM+ CNDF FLGT+  S+GRVG+DFW  DP+GDP G +WLQG HM+HCAYNS
Sbjct: 526 FNGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNS 585

Query: 482 MWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIP 541
           +W+G  IQPDWDMFQS H CA+FHA SRAI GGP+Y+SD VG H+FDL K+LV P+G+I 
Sbjct: 586 LWMGNFIQPDWDMFQSTHPCAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSIL 645

Query: 542 RCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPEC 601
           RC+++ALPTRD LF++ L DG+T+LKIWNLNKY GVIGAFNCQG GW  E RR +   EC
Sbjct: 646 RCEYYALPTRDRLFEDPLHDGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSEC 705

Query: 602 YKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFE 661
              ++ T    DVEW+       + N  E+ ++L+Q++++L  +  ++ LE+T++P  FE
Sbjct: 706 VNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFE 765

Query: 662 LFSFIPLRKL-GSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSP 721
           L +  P+  + G+S++FAPIGL NM N+SG I+ L YN+  VE+ V G G F  Y+S  P
Sbjct: 766 LITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKP 783

Query: 722 KQCLSNGMEVEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
             CL +G  VEF ++ D  + + +PW+G  DG+S++   F
Sbjct: 826 VSCLIDGEVVEFGYE-DSMVMVQVPWSG-PDGLSSIQYLF 783

BLAST of Moc09g06210 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 5.4e-192
Identity = 343/747 (45.92%), Postives = 453/747 (60.64%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVIL--NVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGST 61
           W+G +G +LQ ETQ +IL  N+   + YV+ +PI+E SFR++L PG +  V + VESGST
Sbjct: 124 WVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQPGLNDYVDMSVESGST 183

Query: 62  HVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAF 121
           HV  S+F A  Y+H+S++PY L+KEA   I+  L TF+ LE K    +++KFGWCTWDAF
Sbjct: 184 HVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGWCTWDAF 243

Query: 122 YLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDP--NRDAKNLVLCGTQMTA 181
           YL V P G+W G+      G  P F+IIDDGWQSI+ D DDP   RD  N    G QM  
Sbjct: 244 YLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPVTERDGMNRTSAGEQMPC 303

Query: 182 RLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEI 241
           RL ++EE  KFR+Y+ G                                           
Sbjct: 304 RLIKYEENYKFREYENG------------------------------------------- 363

Query: 242 SKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDD 301
                          D  GK+                     G+  FVRDLK +F+ ++ 
Sbjct: 364 ---------------DNGGKK---------------------GLVGFVRDLKEEFRSVES 423

Query: 302 VFVWHALAGAWGGVRPGATHL-SSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQA 361
           V+VWHAL G WGGVRP    +  + +   KLSPG+  TM+DLAV KI +  +GLV PN A
Sbjct: 424 VYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENGVGLVPPNLA 483

Query: 362 DDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTG 421
            + FD +HS+L   GI GVKVDVI+ LE +SEE+GGRV+LAKAYYK LT+S+ K+FKG G
Sbjct: 484 QEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSSVNKHFKGNG 543

Query: 422 LFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQ 481
           + +SM+ CNDFF LGT+  S+GRVG+DFW  DP+GDP G YWLQG HM+HCAYNS+W+G 
Sbjct: 544 VIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHCAYNSLWMGN 603

Query: 482 IIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHF 541
            I PDWDMFQS H CA+FHA SRAI GGPVYVSD VG HNF L K  V PDG+I RCQH+
Sbjct: 604 FIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPDGSILRCQHY 663

Query: 542 ALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMS 601
           ALPTRDCLF++ L +G+T+LKIWNLNKY GV+G FNCQG GW PE RR K   E    ++
Sbjct: 664 ALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKSASEFSHAVT 723

Query: 602 MTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFI 661
                 D+EW        +     + VY  + E+ L     S+ LEV+++P +FEL +  
Sbjct: 724 CYASPEDIEWCNGKTPMDIKGVDVFAVYFFK-EKKLSLMKCSDRLEVSLEPFSFELMTVS 783

Query: 662 PLRKLGSS-IKFAPIGLTNMFNSSGTIQHLKYNENG--VELKVKGGGSFLAYSSGSPKQC 721
           PL+      I+FAPIGL NM NS G +Q L+++++   V++ V+G G    ++S  P  C
Sbjct: 784 PLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFASEKPVCC 789

Query: 722 LSNGMEVEFEWDSDGKLGLDLPWNGEA 741
             +G+ VEF+++ D  + + + W G +
Sbjct: 844 KIDGVSVEFDYE-DKMVRVQILWPGSS 789

BLAST of Moc09g06210 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 4.9e-185
Identity = 332/767 (43.29%), Postives = 447/767 (58.28%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKS-------YVIFIPIIEGSFRSALHPGD-DGQVFIC 61
           W+G +G D++ ETQ +IL+    KS       YV+ +PI+EG FR+ L  G  +  V + 
Sbjct: 110 WVGTNGRDVENETQMMILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLESGKAEDYVHMV 169

Query: 62  VESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGW 121
           +ESGS+ V+ S F +  Y+H  D+P++L+K+A   +R HL TFRL+E K    +VDKFGW
Sbjct: 170 LESGSSTVRGSVFRSAVYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGW 229

Query: 122 CTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAK--NLVLC 181
           CTWDAFYL V P G+W G+   A  G  P  ++IDDGWQSI  D DD    A+  N    
Sbjct: 230 CTWDAFYLKVHPEGVWEGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSGAEGMNRTSA 289

Query: 182 GTQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIA 241
           G QM  RL +F+E  KFR+YKG                                      
Sbjct: 290 GEQMPCRLIKFQENYKFREYKG-------------------------------------- 349

Query: 242 SGVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTK 301
                                                           GM  FVR++K  
Sbjct: 350 ------------------------------------------------GMGGFVREMKAA 409

Query: 302 FKGLDDVFVWHALAGAWGGVRPGATHL-SSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGL 361
           F  ++ V+VWHAL G WGG+RPGA  L  + +   +LSPGL  TM+DLAV KI +  +GL
Sbjct: 410 FPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGL 469

Query: 362 VHPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILK 421
           V P +A + ++ +HS+L   GI GVKVDVI+ LE V EE+GGRV+LAKAY+ GLT S+ +
Sbjct: 470 VDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEMVCEEYGGRVELAKAYFAGLTESVRR 529

Query: 422 NFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYN 481
           +F G G+ +SM+ CNDF  LGT+  ++GRVG+DFW  DP+GDP G +WLQG HM+HCAYN
Sbjct: 530 HFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYN 589

Query: 482 SMWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTI 541
           S+W+G  I PDWDMFQS H CA FHA SRA+ GGPVYVSD+VG H+FDL ++L  PDGTI
Sbjct: 590 SLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTI 649

Query: 542 PRCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPE 601
            RC+ +ALPTRDCLF + L DG+T+LKIWN+NK+ GV+GAFNCQG GW  E RR      
Sbjct: 650 LRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFSGVLGAFNCQGGGWSREARRNMCAAG 709

Query: 602 CYKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTF 661
              P++     +DVEW         G    + VY  +A + L    + E +E+T++P T+
Sbjct: 710 FSVPVTARASPADVEWSHGG-----GGGDRFAVYFVEARK-LQLLRRDESVELTLEPFTY 769

Query: 662 ELFSFIPLRKLGS---SIKFAPIGLTNMFNSSGTIQHL----KYNENGVELKVKGGGSFL 721
           EL    P+R + S    I FAPIGL NM N+ G +Q      K  +   E+ VKG G  +
Sbjct: 770 ELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEMV 783

Query: 722 AYSSGSPKQCLSNGMEVEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           AYSS  P+ C  NG + EF+++ DG + +D+PW G +  +S ++ F+
Sbjct: 830 AYSSARPRLCKVNGQDAEFKYE-DGIVTVDVPWTGSSKKLSRVEYFY 783

BLAST of Moc09g06210 vs. ExPASy TrEMBL
Match: A0A6J1D420 (stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111016788 PE=3 SV=1)

HSP 1 Score: 1467.6 bits (3798), Expect = 0.0e+00
Identity = 685/750 (91.33%), Postives = 721/750 (96.13%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MWIGNSGSDLQMETQWVILNVPE+KSYV+FIPIIEGSFRSALHPG DGQV IC ESGSTH
Sbjct: 114 MWIGNSGSDLQMETQWVILNVPEIKSYVVFIPIIEGSFRSALHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAAIRVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKVSSFDAIAYVHVSDNPYKLMKEAYAAIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDPIGIWNG+NDFA+ GISPRFLIIDDGWQSINMD +DPNRD KNL+L GTQMT+RLY
Sbjct: 234 LTVDPIGIWNGVNDFAEGGISPRFLIIDDGWQSINMDDEDPNRDGKNLILGGTQMTSRLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RFEE EKFRKYKGGSLLGPNAPSF+PK+PKLLIAKAIE+EHAE+DRD+AIASGVN+ SKF
Sbjct: 294 RFEECEKFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIELEHAERDRDEAIASGVNDTSKF 353

Query: 241 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 300
           EIKIQKLKEE+D+IFGKEDEESGAV KGCSSCSCKAD+SGMKAF RDLKTKFKGLDD+FV
Sbjct: 354 EIKIQKLKEEVDEIFGKEDEESGAVTKGCSSCSCKADNSGMKAFTRDLKTKFKGLDDIFV 413

Query: 301 WHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFF 360
           WHALAGAWGGVRPGATHLSS + PCKLSPGL  TM DLAVVKI +GSIGLVHPNQADDFF
Sbjct: 414 WHALAGAWGGVRPGATHLSSKIVPCKLSPGLDGTMTDLAVVKIVEGSIGLVHPNQADDFF 473

Query: 361 DSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSS 420
           DSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFSS
Sbjct: 474 DSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSS 533

Query: 421 MQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQP 480
           MQQCNDFFFLGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQP
Sbjct: 534 MQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQP 593

Query: 481 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPT 540
           DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALPT
Sbjct: 594 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPT 653

Query: 541 RDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIH 600
           RDCLFKN+LFDG+TVLKIWNLNKYGGVIGAFNCQGAGWDP+E+RIKGHPECYKP S T+H
Sbjct: 654 RDCLFKNSLFDGKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPTSTTVH 713

Query: 601 VSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRK 660
           VSDVEWDQK EAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+VT+QPSTFELFSFIPLRK
Sbjct: 714 VSDVEWDQKQEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRK 773

Query: 661 LGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEV 720
           LGSSIKFAPIGLTNMFNSSGTIQHLKYNE GVELKVKGGGSFLAYS+ SPK+C+SNGMEV
Sbjct: 774 LGSSIKFAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGGGSFLAYSNASPKKCVSNGMEV 833

Query: 721 EFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           EFEWDSDGKLG DLPWNGEA GVSNLDIFF
Sbjct: 834 EFEWDSDGKLGFDLPWNGEAGGVSNLDIFF 863

BLAST of Moc09g06210 vs. ExPASy TrEMBL
Match: A0A6J1D2W3 (stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111016796 PE=3 SV=1)

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 674/750 (89.87%), Postives = 717/750 (95.60%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSD+QMETQWV+LNVPE+KSYV+FIPIIEGSFRSAL+PG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDIQMETQWVMLNVPEIKSYVVFIPIIEGSFRSALYPGADGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVS+NPYNLMKEAYAAIRVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKESSFDAIAYVHVSNNPYNLMKEAYAAIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP GIWNGLNDFA+ GISPRFLIIDDGWQSINMD +DPN+D K+LVLCGTQMT+RLY
Sbjct: 234 LTVDPTGIWNGLNDFAEGGISPRFLIIDDGWQSINMDDEDPNQDGKHLVLCGTQMTSRLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           +FEE EKF+KYKGGS LGPN PSFNPKRPKLLI KA EIE A++DRD+AIA GVN++SKF
Sbjct: 294 KFEECEKFKKYKGGSFLGPNVPSFNPKRPKLLIQKADEIEDAKRDRDEAIAYGVNDVSKF 353

Query: 241 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 300
           EIKIQKLKEELDDIFGKEDEESGAV KGCS+ SCKA DSGMKAF RDLKTKFKGLDDVFV
Sbjct: 354 EIKIQKLKEELDDIFGKEDEESGAVTKGCSTGSCKAADSGMKAFTRDLKTKFKGLDDVFV 413

Query: 301 WHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDFF 360
           WHALAGAWGGVRPGATHL+SNL PCKLSPGL  TMDDLAVVKI +GSIGLVHPNQADDFF
Sbjct: 414 WHALAGAWGGVRPGATHLNSNLIPCKLSPGLDGTMDDLAVVKIIEGSIGLVHPNQADDFF 473

Query: 361 DSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFSS 420
           DSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS++KNFKGTGLFSS
Sbjct: 474 DSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLIKNFKGTGLFSS 533

Query: 421 MQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQP 480
           MQQCNDFFFLGTKQNSIGRVG+DFWFQDPNGDPMGIYWLQGVHMIHCAYNSMW+GQIIQP
Sbjct: 534 MQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWMGQIIQP 593

Query: 481 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALPT 540
           DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALPT
Sbjct: 594 DWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPT 653

Query: 541 RDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTIH 600
           RDC+FKN+LFDG+TVLKIWNLNKYGGVIGAFNCQGAGWDP+ERRIKGHP+CYKPMS TIH
Sbjct: 654 RDCIFKNSLFDGKTVLKIWNLNKYGGVIGAFNCQGAGWDPKERRIKGHPKCYKPMSTTIH 713

Query: 601 VSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLRK 660
           VSDVEWDQKPEAAPMGNFVEYIVYL+QAEQILHTT KSEPL+ ++QPSTFELFSFIPLRK
Sbjct: 714 VSDVEWDQKPEAAPMGNFVEYIVYLSQAEQILHTTTKSEPLKASLQPSTFELFSFIPLRK 773

Query: 661 LGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGMEV 720
           LGSSIKFAPIGLTNMFNSSGTIQHLKYNE GVELKVKGGGSFLAYS+ SPK+C+SNGMEV
Sbjct: 774 LGSSIKFAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGGGSFLAYSNVSPKKCVSNGMEV 833

Query: 721 EFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           EFEW+SDGKLGLDLPWNGEA GVS+LDI F
Sbjct: 834 EFEWNSDGKLGLDLPWNGEAGGVSHLDIIF 863

BLAST of Moc09g06210 vs. ExPASy TrEMBL
Match: B8LG99 (Stachyose synthase OS=Cucumis sativus OX=3659 GN=Csa_7G407800 PE=2 SV=1)

HSP 1 Score: 1418.3 bits (3670), Expect = 0.0e+00
Identity = 657/751 (87.48%), Postives = 707/751 (94.14%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSA+HPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKTSSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG++DF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GPNAPSF+PK+PKLLIAKAIEIEHAEK+RDKAI SGV  +SKF
Sbjct: 294 RFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKF 353

Query: 241 EIKIQKLKEELDDIFGK-EDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KIQKLKEEL  IFGK E+EES A+ KGC+SCSCKAD+SGMKAF RDL+TKFKGLDD+F
Sbjct: 354 ETKIQKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPGATHL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKGHPECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFN SGTIQHLKYNENGVELKVKGGG+FLAYSSGSPK+C+SNG+E
Sbjct: 774 KLGSNIKFAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           VEFEW SDGKL  DL W  EA GVSNLDIFF
Sbjct: 834 VEFEWKSDGKLSFDLHWIEEAGGVSNLDIFF 864

BLAST of Moc09g06210 vs. ExPASy TrEMBL
Match: A8CM21 (Stachyose synthetase variant 1 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 656/751 (87.35%), Postives = 706/751 (94.01%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSA+HPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VK SSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKTSSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG++DF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GPNAPSF+PK+PKLLIAKAIEIEHAEK+RDKAI SGV  +SKF
Sbjct: 294 RFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKF 353

Query: 241 EIKIQKLKEELDDIFGK-EDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KIQKLKEEL  IFGK E+EES A+ KGC+SCSCKAD+SGMKAF RDL+TKFKGLDD+F
Sbjct: 354 ETKIQKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPGATHL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKGHPECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFN SGTIQHLKYNENGVELKVKGGG+FLAYSSGSPK+C+SNG+E
Sbjct: 774 KLGSNIKFAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           VEFEW SDGKL  DL W  EA GV NLDIFF
Sbjct: 834 VEFEWKSDGKLSFDLHWIEEAGGVFNLDIFF 864

BLAST of Moc09g06210 vs. ExPASy TrEMBL
Match: A0A5A7TXN2 (Stachyose synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001820 PE=3 SV=1)

HSP 1 Score: 1415.2 bits (3662), Expect = 0.0e+00
Identity = 655/751 (87.22%), Postives = 709/751 (94.41%), Query Frame = 0

Query: 1   MWIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTH 60
           MW+GNSGSDLQMETQWV+LN+PE+KSYV+ IPIIEGSFRSALHPG DGQV IC ESGSTH
Sbjct: 114 MWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTH 173

Query: 61  VKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFY 120
           VKASSFDAIAYVHVSDNPY LMKEAYAA+RVHLNTFRLLE KPVTHLVDKFGWCTWDAFY
Sbjct: 174 VKASSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFY 233

Query: 121 LTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLY 180
           LTVDP+GIWNG+NDF + GISPRFLIIDDGWQSIN+DG+DP RDAKNLVL GTQMTARLY
Sbjct: 234 LTVDPVGIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY 293

Query: 181 RFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIASGVNEISKF 240
           RF+E EKFRKYKGGSL GPNAPSF+PK+PKLLIAKAIEIEHAEK+RDKAI SGV  +SKF
Sbjct: 294 RFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKF 353

Query: 241 EIKIQKLKEELDDIFG-KEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVF 300
           E KIQKLKEEL +IFG +E+EES A+ KGC+SCSCKAD+SGMKAF RDL+TKFKGLDD+F
Sbjct: 354 ETKIQKLKEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIF 413

Query: 301 VWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 360
           VWHALAGAWGGVRPGATHL+S + PCKLSPGL  TM DLAVVKI +GSIGLVHP+QADDF
Sbjct: 414 VWHALAGAWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDF 473

Query: 361 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 420
           FDSMHSYLSKVGI GVKVDV++TLEYVSEE+GGRVDLAKAYYKGLTNS+LKNFKGTGLFS
Sbjct: 474 FDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFS 533

Query: 421 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 480
           SMQQCNDFF+LGTKQNSIGRVG+DFWFQDPNGDPMG+YWLQGVHMIHCAYNSMW+GQIIQ
Sbjct: 534 SMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQ 593

Query: 481 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIPRCQHFALP 540
           PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDL KQLVYPDGTIPRCQHFALP
Sbjct: 594 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALP 653

Query: 541 TRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSMTI 600
           TRDCLFKN LFD +TVLKIWNLNKYGGVIG FNCQGAGWDP+E+RIKGHPECYKPMS T+
Sbjct: 654 TRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTV 713

Query: 601 HVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELFSFIPLR 660
           HV+D+EWDQKPEAAPMGNFVEYIVYLNQAEQI+HTTPKSEPL+ T+QPSTFELF+FIPLR
Sbjct: 714 HVNDIEWDQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLR 773

Query: 661 KLGSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKQCLSNGME 720
           KLGS+IKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGG+FLAYSSGSPK+CLSNG E
Sbjct: 774 KLGSNIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTE 833

Query: 721 VEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
           ++F W+SDGKL  D+ W  EA G+SNLDIFF
Sbjct: 834 LKFVWNSDGKLSFDVHWIEEAGGISNLDIFF 864

BLAST of Moc09g06210 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 944.5 bits (2440), Expect = 5.2e-275
Identity = 447/760 (58.82%), Postives = 571/760 (75.13%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKSYVIFIPIIEGSFRSALHPGDDGQVFICVESGSTHV 61
           WIG SGSDLQ ETQWV+L +PE+ SYV  IP IEG+FR++L PG+ G V IC ESGST V
Sbjct: 126 WIGKSGSDLQAETQWVMLKIPEIDSYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKV 185

Query: 62  KASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFGWCTWDAFYL 121
           K SSF +IAY+H+ DNPYNLMKEA++A+RVH+NTF+LLE K +  +VDKFGWCTWDA YL
Sbjct: 186 KESSFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYL 245

Query: 122 TVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCGTQMTARLYR 181
           TVDP  IW G+ +F   G+ P+F+IIDDGWQSIN DGD+ ++DA+NLVL G QMTARL  
Sbjct: 246 TVDPATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTS 305

Query: 182 FEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAI-ASGVNEISKF 241
           F+E +KFR YKGGS +  +A  FNP +PK+LI KA E   A   R K +  SG  ++++ 
Sbjct: 306 FKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTEL 365

Query: 242 EIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKFKGLDDVFV 301
           + KI+ L EEL+ +F + ++E        S  S     SGM AF +DL+ +FK LDD++V
Sbjct: 366 DEKIKILSEELNAMFDEVEKEE-------SLGSDDVSGSGMAAFTKDLRLRFKSLDDIYV 425

Query: 302 WHALAGAWGGVRPGA-THLSSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLVHPNQADDF 361
           WHAL GAW GVRP     L + + P +LSP LG TM DLAV K+ +  IGLVHP++A +F
Sbjct: 426 WHALCGAWNGVRPETMMDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEF 485

Query: 362 FDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKNFKGTGLFS 421
           +DSMHSYL+ VG+ G K+DV  TLE ++EEHGGRV+LAKAYY GLT S++KNF GT + +
Sbjct: 486 YDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIA 545

Query: 422 SMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNSMWIGQIIQ 481
           SMQQCN+FFFL TKQ SIGRVG+DFW+QDP GDP G+YWLQGVHMIHC+YNS+W+GQ+IQ
Sbjct: 546 SMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQ 605

Query: 482 PDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVG--GHNFDLKKQLVYPDGTIPRCQHFA 541
           PDWDMFQSDH+CA++HA SRAICGGPVY+SD +G   HNFDL K+L + DGTIPRC H+A
Sbjct: 606 PDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYA 665

Query: 542 LPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPECYKPMSM 601
           LPTRD LFKN LFD  ++LKI+N NK+GGVIG FNCQGAGW PEE R KG+ ECY  +S 
Sbjct: 666 LPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSG 725

Query: 602 TIHVSDVEWDQKPEAAPMGNFV----EYIVYLNQAEQILHTTPKSEPLEVTVQPSTFELF 661
           T+HVSD+EWDQ PEAA  G+ V    +Y+VY  Q+E+IL    KSE +++T++PS F+L 
Sbjct: 726 TVHVSDIEWDQNPEAA--GSQVTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLL 785

Query: 662 SFIPLRKLGSS-IKFAPIGLTNMFNSSGTIQHLKY-NENGVELKVKGGGSFLAYSSGSPK 721
           SF+P+ +L SS ++FAP+GL NMFN  GT+Q +K   +N + + VKG G F+AYSS +P 
Sbjct: 786 SFVPVTELVSSGVRFAPLGLINMFNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPV 845

Query: 722 QCLSNGMEVEFEWDSD-GKLGLDLPWNGEADGVSNLDIFF 751
           +C  N  E EF+W+ + GKL   +PW  E+ G+S+L   F
Sbjct: 846 KCYLNDKEAEFKWEEETGKLSFFVPWVEESGGISHLSFTF 876

BLAST of Moc09g06210 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 698.0 bits (1800), Expect = 8.4e-201
Identity = 348/760 (45.79%), Postives = 471/760 (61.97%), Query Frame = 0

Query: 2   WIGNSGSDLQMETQWVILNVPEVKS---------YVIFIPIIEGSFRSALHPGDDGQVFI 61
           W+G++G D++ ETQ +IL+     S         YV+ +P++EGSFRS+   G+D  V +
Sbjct: 106 WVGSNGRDIENETQIIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAV 165

Query: 62  CVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHLVDKFG 121
           CVESGST V  S F  I YVH  D+P+ L+K+A   IRVH+NTF+LLE K    +VDKFG
Sbjct: 166 CVESGSTEVTGSEFRQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFG 225

Query: 122 WCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKNLVLCG 181
           WCTWDAFYLTV+P G+  G+      G  P  ++IDDGWQSI  D D  + +  N+ + G
Sbjct: 226 WCTWDAFYLTVNPDGVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAG 285

Query: 182 TQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRDKAIAS 241
            QM  RL +FEE  KF+ Y             +PK                         
Sbjct: 286 EQMPCRLLKFEENHKFKDY------------VSPK------------------------- 345

Query: 242 GVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRDLKTKF 301
                                                       +D GMKAFVRDLK +F
Sbjct: 346 ------------------------------------------DQNDVGMKAFVRDLKDEF 405

Query: 302 KGLDDVFVWHALAGAWGGVRPGATHL-SSNLTPCKLSPGLGDTMDDLAVVKIFDGSIGLV 361
             +D ++VWHAL G WGG+RP A  L  S +   +LSPGL  TM+DLAV KI +  IG  
Sbjct: 406 STVDYIYVWHALCGYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFA 465

Query: 362 HPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNSILKN 421
            P+ A +F++ +HS+L   GI GVKVDVI+ LE + +++GGRVDLAKAY+K LT+S+ K+
Sbjct: 466 SPDLAKEFYEGLHSHLQNAGIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKH 525

Query: 422 FKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHCAYNS 481
           F G G+ +SM+ CNDF FLGT+  S+GRVG+DFW  DP+GDP G +WLQG HM+HCAYNS
Sbjct: 526 FNGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNS 585

Query: 482 MWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPDGTIP 541
           +W+G  IQPDWDMFQS H CA+FHA SRAI GGP+Y+SD VG H+FDL K+LV P+G+I 
Sbjct: 586 LWMGNFIQPDWDMFQSTHPCAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSIL 645

Query: 542 RCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKGHPEC 601
           RC+++ALPTRD LF++ L DG+T+LKIWNLNKY GVIGAFNCQG GW  E RR +   EC
Sbjct: 646 RCEYYALPTRDRLFEDPLHDGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSEC 705

Query: 602 YKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQPSTFE 661
              ++ T    DVEW+       + N  E+ ++L+Q++++L  +  ++ LE+T++P  FE
Sbjct: 706 VNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFE 765

Query: 662 LFSFIPLRKL-GSSIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSP 721
           L +  P+  + G+S++FAPIGL NM N+SG I+ L YN+  VE+ V G G F  Y+S  P
Sbjct: 766 LITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKP 783

Query: 722 KQCLSNGMEVEFEWDSDGKLGLDLPWNGEADGVSNLDIFF 751
             CL +G  VEF ++ D  + + +PW+G  DG+S++   F
Sbjct: 826 VSCLIDGEVVEFGYE-DSMVMVQVPWSG-PDGLSSIQYLF 783

BLAST of Moc09g06210 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 9.6e-120
Identity = 256/745 (34.36%), Postives = 374/745 (50.20%), Query Frame = 0

Query: 3   IGNSGSDLQMETQWVILNVPE---------------VKSYVIFIPIIEGSFRSALHPGDD 62
           +G  G D+  ETQ++++   +                K Y +F+P+IEGSFRS L    +
Sbjct: 83  MGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVN 142

Query: 63  GQVFICVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHL 122
            +V +C+ESG    K SSF    Y+H   +P+  + +A   +++HLN+FR    K +  +
Sbjct: 143 DEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGI 202

Query: 123 VDKFGWCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKN 182
           VD FGWCTWDAFY  V   G+  GL   A  G  P+F+IIDDGWQS+  D      D K 
Sbjct: 203 VDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK- 262

Query: 183 LVLCGTQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRD 242
                          +E+  FR                                      
Sbjct: 263 ---------------KESPIFR-------------------------------------- 322

Query: 243 KAIASGVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRD 302
               +G+ E  KF+               K+D+                 + G+K  V+ 
Sbjct: 323 ---LTGIKENEKFK---------------KKDD----------------PNVGIKNIVKI 382

Query: 303 LKTKFKGLDDVFVWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGS 362
            K K  GL  V+VWHA+ G WGGVRPG  + S    P  +S G+ +         +    
Sbjct: 383 AKEK-HGLKYVYVWHAITGYWGGVRPGEEYGSVMKYP-NMSKGVVENDPTWKTDVMTLQG 442

Query: 363 IGLVHPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNS 422
           +GLV P +   F++ +HSYL+  G+ GVKVDV   LE +    GGRV+L + +++ L +S
Sbjct: 443 LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSS 502

Query: 423 ILKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHC 482
           + KNF   G  + M    D  +  +KQ ++ R  +DF+ +DP            +H+   
Sbjct: 503 VAKNFPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVS--------HTIHIASV 562

Query: 483 AYNSMWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPD 542
           AYNS+++G+ +QPDWDMF S H  A++HA +RAI GGP+YVSDS G HNF+L ++LV PD
Sbjct: 563 AYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPD 622

Query: 543 GTIPRCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKG 602
           G+I R +    PTRDCLF +   DG ++LKIWN+NKY GV+G +NCQGA W   ER+   
Sbjct: 623 GSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIF 682

Query: 603 HPECYKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQP 662
           H      ++ +I   DV    +    P     +  VY +Q+   L   P +  L V+++ 
Sbjct: 683 HQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKI 727

Query: 663 STFELFSFIPLRKLGSSIKFAPIGLTNMFNSSGTIQHLKY--NENGVELKVKGGGSFLAY 722
              E+F+  P+  L   + FAPIGL NM+NS G I+ L+Y   +  V ++VKG G F +Y
Sbjct: 743 REHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSY 727

Query: 723 SSGSPKQCLSNGMEVEFEWDSDGKL 731
           SS  PK+C+    E+ FE+DS   L
Sbjct: 803 SSVKPKRCVVESNEIAFEYDSSSGL 727

BLAST of Moc09g06210 vs. TAIR 10
Match: AT5G20250.2 (Raffinose synthase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 9.6e-120
Identity = 256/745 (34.36%), Postives = 374/745 (50.20%), Query Frame = 0

Query: 3   IGNSGSDLQMETQWVILNVPE---------------VKSYVIFIPIIEGSFRSALHPGDD 62
           +G  G D+  ETQ++++   +                K Y +F+P+IEGSFRS L    +
Sbjct: 83  MGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVN 142

Query: 63  GQVFICVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHL 122
            +V +C+ESG    K SSF    Y+H   +P+  + +A   +++HLN+FR    K +  +
Sbjct: 143 DEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGI 202

Query: 123 VDKFGWCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKN 182
           VD FGWCTWDAFY  V   G+  GL   A  G  P+F+IIDDGWQS+  D      D K 
Sbjct: 203 VDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK- 262

Query: 183 LVLCGTQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRD 242
                          +E+  FR                                      
Sbjct: 263 ---------------KESPIFR-------------------------------------- 322

Query: 243 KAIASGVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRD 302
               +G+ E  KF+               K+D+                 + G+K  V+ 
Sbjct: 323 ---LTGIKENEKFK---------------KKDD----------------PNVGIKNIVKI 382

Query: 303 LKTKFKGLDDVFVWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGS 362
            K K  GL  V+VWHA+ G WGGVRPG  + S    P  +S G+ +         +    
Sbjct: 383 AKEK-HGLKYVYVWHAITGYWGGVRPGEEYGSVMKYP-NMSKGVVENDPTWKTDVMTLQG 442

Query: 363 IGLVHPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNS 422
           +GLV P +   F++ +HSYL+  G+ GVKVDV   LE +    GGRV+L + +++ L +S
Sbjct: 443 LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSS 502

Query: 423 ILKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHC 482
           + KNF   G  + M    D  +  +KQ ++ R  +DF+ +DP            +H+   
Sbjct: 503 VAKNFPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVS--------HTIHIASV 562

Query: 483 AYNSMWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPD 542
           AYNS+++G+ +QPDWDMF S H  A++HA +RAI GGP+YVSDS G HNF+L ++LV PD
Sbjct: 563 AYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPD 622

Query: 543 GTIPRCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKG 602
           G+I R +    PTRDCLF +   DG ++LKIWN+NKY GV+G +NCQGA W   ER+   
Sbjct: 623 GSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIF 682

Query: 603 HPECYKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQP 662
           H      ++ +I   DV    +    P     +  VY +Q+   L   P +  L V+++ 
Sbjct: 683 HQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKI 727

Query: 663 STFELFSFIPLRKLGSSIKFAPIGLTNMFNSSGTIQHLKY--NENGVELKVKGGGSFLAY 722
              E+F+  P+  L   + FAPIGL NM+NS G I+ L+Y   +  V ++VKG G F +Y
Sbjct: 743 REHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSY 727

Query: 723 SSGSPKQCLSNGMEVEFEWDSDGKL 731
           SS  PK+C+    E+ FE+DS   L
Sbjct: 803 SSVKPKRCVVESNEIAFEYDSSSGL 727

BLAST of Moc09g06210 vs. TAIR 10
Match: AT5G20250.3 (Raffinose synthase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 9.6e-120
Identity = 256/745 (34.36%), Postives = 374/745 (50.20%), Query Frame = 0

Query: 3   IGNSGSDLQMETQWVILNVPE---------------VKSYVIFIPIIEGSFRSALHPGDD 62
           +G  G D+  ETQ++++   +                K Y +F+P+IEGSFRS L    +
Sbjct: 83  MGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVN 142

Query: 63  GQVFICVESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYAAIRVHLNTFRLLEGKPVTHL 122
            +V +C+ESG    K SSF    Y+H   +P+  + +A   +++HLN+FR    K +  +
Sbjct: 143 DEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGI 202

Query: 123 VDKFGWCTWDAFYLTVDPIGIWNGLNDFAKEGISPRFLIIDDGWQSINMDGDDPNRDAKN 182
           VD FGWCTWDAFY  V   G+  GL   A  G  P+F+IIDDGWQS+  D      D K 
Sbjct: 203 VDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK- 262

Query: 183 LVLCGTQMTARLYRFEEAEKFRKYKGGSLLGPNAPSFNPKRPKLLIAKAIEIEHAEKDRD 242
                          +E+  FR                                      
Sbjct: 263 ---------------KESPIFR-------------------------------------- 322

Query: 243 KAIASGVNEISKFEIKIQKLKEELDDIFGKEDEESGAVIKGCSSCSCKADDSGMKAFVRD 302
               +G+ E  KF+               K+D+                 + G+K  V+ 
Sbjct: 323 ---LTGIKENEKFK---------------KKDD----------------PNVGIKNIVKI 382

Query: 303 LKTKFKGLDDVFVWHALAGAWGGVRPGATHLSSNLTPCKLSPGLGDTMDDLAVVKIFDGS 362
            K K  GL  V+VWHA+ G WGGVRPG  + S    P  +S G+ +         +    
Sbjct: 383 AKEK-HGLKYVYVWHAITGYWGGVRPGEEYGSVMKYP-NMSKGVVENDPTWKTDVMTLQG 442

Query: 363 IGLVHPNQADDFFDSMHSYLSKVGIAGVKVDVINTLEYVSEEHGGRVDLAKAYYKGLTNS 422
           +GLV P +   F++ +HSYL+  G+ GVKVDV   LE +    GGRV+L + +++ L +S
Sbjct: 443 LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSS 502

Query: 423 ILKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGEDFWFQDPNGDPMGIYWLQGVHMIHC 482
           + KNF   G  + M    D  +  +KQ ++ R  +DF+ +DP            +H+   
Sbjct: 503 VAKNFPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVS--------HTIHIASV 562

Query: 483 AYNSMWIGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLKKQLVYPD 542
           AYNS+++G+ +QPDWDMF S H  A++HA +RAI GGP+YVSDS G HNF+L ++LV PD
Sbjct: 563 AYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPD 622

Query: 543 GTIPRCQHFALPTRDCLFKNTLFDGRTVLKIWNLNKYGGVIGAFNCQGAGWDPEERRIKG 602
           G+I R +    PTRDCLF +   DG ++LKIWN+NKY GV+G +NCQGA W   ER+   
Sbjct: 623 GSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIF 682

Query: 603 HPECYKPMSMTIHVSDVEWDQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLEVTVQP 662
           H      ++ +I   DV    +    P     +  VY +Q+   L   P +  L V+++ 
Sbjct: 683 HQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKI 727

Query: 663 STFELFSFIPLRKLGSSIKFAPIGLTNMFNSSGTIQHLKY--NENGVELKVKGGGSFLAY 722
              E+F+  P+  L   + FAPIGL NM+NS G I+ L+Y   +  V ++VKG G F +Y
Sbjct: 743 REHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSY 727

Query: 723 SSGSPKQCLSNGMEVEFEWDSDGKL 731
           SS  PK+C+    E+ FE+DS   L
Sbjct: 803 SSVKPKRCVVESNEIAFEYDSSSGL 727

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148001.10.0e+0091.33stachyose synthase-like [Momordica charantia][more]
XP_022148013.10.0e+0089.87stachyose synthase-like [Momordica charantia][more]
XP_038897999.10.0e+0087.75stachyose synthase [Benincasa hispida][more]
NP_001267675.10.0e+0087.48steryl-sulfatase [Cucumis sativus] >ABQ53598.1 stachyose synthase [Cucumis sativ... [more]
ABV44498.10.0e+0087.35stachyose synthetase variant 1 [Cucumis sativus] >ABV44499.1 stachyose synthetas... [more]
Match NameE-valueIdentityDescription
Q93XK20.0e+0066.89Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1[more]
Q9SYJ47.3e-27458.82Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=... [more]
Q9FND91.2e-19945.79Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=... [more]
Q8VWN65.4e-19245.92Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 S... [more]
Q5VQG44.9e-18543.29Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1D4200.0e+0091.33stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111016788 PE=3 SV=1[more]
A0A6J1D2W30.0e+0089.87stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111016796 PE=3 SV=1[more]
B8LG990.0e+0087.48Stachyose synthase OS=Cucumis sativus OX=3659 GN=Csa_7G407800 PE=2 SV=1[more]
A8CM210.0e+0087.35Stachyose synthetase variant 1 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A5A7TXN20.0e+0087.22Stachyose synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G0... [more]
Match NameE-valueIdentityDescription
AT4G01970.15.2e-27558.82stachyose synthase [more]
AT5G40390.18.4e-20145.79Raffinose synthase family protein [more]
AT5G20250.19.6e-12034.36Raffinose synthase family protein [more]
AT5G20250.29.6e-12034.36Raffinose synthase family protein [more]
AT5G20250.39.6e-12034.36Raffinose synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 230..257
NoneNo IPR availablePANTHERPTHR31268:SF8GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 4-RELATEDcoord: 1..750
IPR008811Glycosyl hydrolases 36PFAMPF05691Raffinose_syncoord: 2..731
e-value: 9.2E-294
score: 976.0
IPR008811Glycosyl hydrolases 36PANTHERPTHR31268FAMILY NOT NAMEDcoord: 1..750
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 107..524

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g06210.1Moc09g06210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process