Moc09g01220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g01220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Vacuolar-processing enzyme
Location	chr9: 1037723 .. 1039728 (-)
RNA-Seq Expression	Moc09g01220
Synteny	Moc09g01220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACTTGTTATGCAACTTCAACTAAATTTGTTCTTCTCATAGCTCTGCTGTTGTTCAGTGATATTATTGCTAAGAGAGAGAGTGTTGATGGTGCCTCTACCGACCAACCTGGCAAGAGATGGGCAATTTTGGTTGCTGGCTCCAGTGGTTATGAGAATTATAGGCATCAGGTGATTCAATAAGAACCAATTCATAGTCATACCAACATACTGATATTAACTGCGTCTCTTTTTCTTTTCTAGGCGGATGTATGTCATGCATACCAAATACTGAGAAAGGGTGGTCTCCCAGATGAGAACATAATAGTTTTCATGTATGATGATATTGCATTCAATCCAAGCAATCCAAGGCCAGGAGTCGTTATCAACAAACCAGATGGTGTCGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATTCGATCAACTTTTACGCTGTCATTTTGGGCAACAGATCTGCTCTCACTGGAGGAAGTGGTAAGGTTGTCGACAGTGACCTTCACGATCATATTTTCATCTACTACACTGACCACGGTAGCGCTGGGTTACTAGGTAAGTTATACTTACAACTCTTTTGTCTCAACAAAATAGTGTTCTGTTAGAAATTTGACAATATTACTTCACACAACCAAAGGAATGCCCGAGGGTGATTATGTTTACGCCAAGGATCTAATGGAAGTGCTGAAACAAAAGCATGAAGCCAAAAGCTACAAAAGCATGGTACCTAAATTCCATATAATGGCATAGCAGTTATGAAGTTCAAATTATCGAAGTAAAATTATAAGTTGACATAAAAATTCAGGTAATATATGTGGAAGCATGCGAGTCGGGAAGTATGTTAGAAGGATTACTTCCAGAAAACATAAAGATATATGCAACGACGGCATCAAATGCAACAGAGAATAGCTGGGCAACATATTGTCCTGGACAGTTTCCCAGTCCTCCCACAGATTATGATACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGGCAATATTTTCTTTACACCCTCTGCCTATATAACAAAGCTAACTTGTACCTAGCCAATCCCCGCCATATTAAAATGAATTTGTGTTGTGCCATGATATGCAGTGACAAACATGATTTGAGCAAGGAAACTTTAATCCAACAATATGATGCGGTACACATTTTAATTCCTAAATAAGCTCAATTTTTTTAAATGACATGATGAATGTGTTTGTAAATTGCCAAAATGCAGGTTCGAAGAAGAACTTTGGTCGATAAATTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATCGGCAACAATTCCCTTGACACTTATATTGGTGCTAACCCTGATAACTACAACTATACATCTTCTGTCCAGAGTACTGATACTATCGCTCCACCATCAAAGTTGTTGTACTCAAATGCTGTTAGTCAGCGTGATGCTAGTCTCATTCATTACTGGCACAAGGTATGACTTCTTAAACAACAAATATAGCTTTTCTAATCATGACTGCAGTCTACTACATAGTCTAAACGTGTAAAATTTTGCTTTGACAATAATTATCAGTTTCAAAAAGCTCCATTTGGCTCTAGAGAAAAAACGGAGGCTAGAAAGCAGTTAGAGGATGAAATTTTGAATAGAAGGCACGTTGACTCCAGTATATATCACATTGCTAAACTTCTGTTTGGACAAGCAAAGAGCTCAGAAGTGCTGAACAATGTTAGGCAGCAAGGACAATCCCTCGTTGACGATTGGGGCTGCTTCAAGAAGTTTGTAAGTCCTATTTTTAAGACCTGCCTAGCTATATTTTATAACATTAAAAACTCTCCTGATGCAATCTTTAAACCACAGGTGAAAACCTACGAGAAACACTGCAGAAGACTATCAAGATATGGGATGAAATACACACGAGCTCTAGCTAACATATGCAATGCTGGGATTACAATAAACCAAATGGATCAGGCCTGTCTGGAAACTTGCCTTGTAAAAACCTGA

mRNA sequence

ATGGCAACTTGTTATGCAACTTCAACTAAATTTGTTCTTCTCATAGCTCTGCTGTTGTTCAGTGATATTATTGCTAAGAGAGAGAGTGTTGATGGTGCCTCTACCGACCAACCTGGCAAGAGATGGGCAATTTTGGTTGCTGGCTCCAGTGGTTATGAGAATTATAGGCATCAGGCGGATGTATGTCATGCATACCAAATACTGAGAAAGGGTGGTCTCCCAGATGAGAACATAATAGTTTTCATGTATGATGATATTGCATTCAATCCAAGCAATCCAAGGCCAGGAGTCGTTATCAACAAACCAGATGGTGTCGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATTCGATCAACTTTTACGCTGTCATTTTGGGCAACAGATCTGCTCTCACTGGAGGAAGTGGTAAGGTTGTCGACAGTGACCTTCACGATCATATTTTCATCTACTACACTGACCACGGTAGCGCTGGGTTACTAGGAATGCCCGAGGGTGATTATGTTTACGCCAAGGATCTAATGGAAGTGCTGAAACAAAAGCATGAAGCCAAAAGCTACAAAAGCATGGTAATATATGTGGAAGCATGCGAGTCGGGAAGTATGTTAGAAGGATTACTTCCAGAAAACATAAAGATATATGCAACGACGGCATCAAATGCAACAGAGAATAGCTGGGCAACATATTGTCCTGGACAGTTTCCCAGTCCTCCCACAGATTATGATACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGGCAATATTTTCTTTACACCCTCTGCCTATATAACAAAGCTAACTTGTACCTAGCCAATCCCCGCCATATTAAAATGAATTTGTGTTGTGCCATGATATGCAGTGACAAACATGATTTGAGCAAGGAAACTTTAATCCAACAATATGATGCGGTTCGAAGAAGAACTTTGGTCGATAAATTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATCGGCAACAATTCCCTTGACACTTATATTGGTGCTAACCCTGATAACTACAACTATACATCTTCTGTCCAGAGTACTGATACTATCGCTCCACCATCAAAGTTGTTGTACTCAAATGCTGTTAGTCAGCGTGATGCTAGTCTCATTCATTACTGGCACAAGTTTCAAAAAGCTCCATTTGGCTCTAGAGAAAAAACGGAGGCTAGAAAGCAGTTAGAGGATGAAATTTTGAATAGAAGGCACGTTGACTCCAGTATATATCACATTGCTAAACTTCTGTTTGGACAAGCAAAGAGCTCAGAAGTGCTGAACAATGTTAGGCAGCAAGGACAATCCCTCGTTGACGATTGGGGCTGCTTCAAGAAGTTTGTGAAAACCTACGAGAAACACTGCAGAAGACTATCAAGATATGGGATGAAATACACACGAGCTCTAGCTAACATATGCAATGCTGGGATTACAATAAACCAAATGGATCAGGCCTGTCTGGAAACTTGCCTTGTAAAAACCTGA

Coding sequence (CDS)

ATGGCAACTTGTTATGCAACTTCAACTAAATTTGTTCTTCTCATAGCTCTGCTGTTGTTCAGTGATATTATTGCTAAGAGAGAGAGTGTTGATGGTGCCTCTACCGACCAACCTGGCAAGAGATGGGCAATTTTGGTTGCTGGCTCCAGTGGTTATGAGAATTATAGGCATCAGGCGGATGTATGTCATGCATACCAAATACTGAGAAAGGGTGGTCTCCCAGATGAGAACATAATAGTTTTCATGTATGATGATATTGCATTCAATCCAAGCAATCCAAGGCCAGGAGTCGTTATCAACAAACCAGATGGTGTCGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAGAGCATGTGAATTCGATCAACTTTTACGCTGTCATTTTGGGCAACAGATCTGCTCTCACTGGAGGAAGTGGTAAGGTTGTCGACAGTGACCTTCACGATCATATTTTCATCTACTACACTGACCACGGTAGCGCTGGGTTACTAGGAATGCCCGAGGGTGATTATGTTTACGCCAAGGATCTAATGGAAGTGCTGAAACAAAAGCATGAAGCCAAAAGCTACAAAAGCATGGTAATATATGTGGAAGCATGCGAGTCGGGAAGTATGTTAGAAGGATTACTTCCAGAAAACATAAAGATATATGCAACGACGGCATCAAATGCAACAGAGAATAGCTGGGCAACATATTGTCCTGGACAGTTTCCCAGTCCTCCCACAGATTATGATACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGGCAATATTTTCTTTACACCCTCTGCCTATATAACAAAGCTAACTTGTACCTAGCCAATCCCCGCCATATTAAAATGAATTTGTGTTGTGCCATGATATGCAGTGACAAACATGATTTGAGCAAGGAAACTTTAATCCAACAATATGATGCGGTTCGAAGAAGAACTTTGGTCGATAAATTCGGTTATGGTTCTCATGTAATGTTGTATGGAAACAAAAGCATCGGCAACAATTCCCTTGACACTTATATTGGTGCTAACCCTGATAACTACAACTATACATCTTCTGTCCAGAGTACTGATACTATCGCTCCACCATCAAAGTTGTTGTACTCAAATGCTGTTAGTCAGCGTGATGCTAGTCTCATTCATTACTGGCACAAGTTTCAAAAAGCTCCATTTGGCTCTAGAGAAAAAACGGAGGCTAGAAAGCAGTTAGAGGATGAAATTTTGAATAGAAGGCACGTTGACTCCAGTATATATCACATTGCTAAACTTCTGTTTGGACAAGCAAAGAGCTCAGAAGTGCTGAACAATGTTAGGCAGCAAGGACAATCCCTCGTTGACGATTGGGGCTGCTTCAAGAAGTTTGTGAAAACCTACGAGAAACACTGCAGAAGACTATCAAGATATGGGATGAAATACACACGAGCTCTAGCTAACATATGCAATGCTGGGATTACAATAAACCAAATGGATCAGGCCTGTCTGGAAACTTGCCTTGTAAAAACCTGA

Protein sequence

MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVKT

Homology

[Close]

BLAST of Moc09g01220 vs. NCBI nr
Match: XP_022148043.1 (vacuolar-processing enzyme-like isoform X1 [Momordica charantia])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.5e-299
Identity = 504/504 (100.00%), Postives = 504/504 (100.00%), Query Frame = 0


Query: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60
           MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120
           VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180
           NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240
           VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300
           PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360
           SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420
           DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480
           YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480

Query: 481 NICNAGITINQMDQACLETCLVKT 505
           NICNAGITINQMDQACLETCLVKT
Sbjct: 481 NICNAGITINQMDQACLETCLVKT 504

[Close]

BLAST of Moc09g01220 vs. NCBI nr
Match: XP_022148051.1 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia])

HSP 1 Score: 947.2 bits (2447), Expect = 5.8e-272
Identity = 470/504 (93.25%), Postives = 470/504 (93.25%), Query Frame = 0


Query: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60
           MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120
           VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180
           NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240
           VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300
           PPTDYDTCLGDLYSIAWMED                                  SDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMED----------------------------------SDKHDL 300

Query: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360
           SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420
           DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480
           YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 470

Query: 481 NICNAGITINQMDQACLETCLVKT 505
           NICNAGITINQMDQACLETCLVKT
Sbjct: 481 NICNAGITINQMDQACLETCLVKT 470

[Close]

BLAST of Moc09g01220 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 710.3 bits (1832), Expect = 1.2e-200
Identity = 353/506 (69.76%), Postives = 408/506 (80.63%), Query Frame = 0


Query: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60
           M TC  + TK  V+LI  + F++IIAK+E +  A+ +Q GKRWA+LVAGS+GYENYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120
           DVCHAYQIL+KGG+PDENIIVFMYDDIAF+P+NPR G++INKP G DVYQGVPKDYTG+ 
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180
           VN++NFYA + GN+SALTGGSGKV+ S  HDHIFIYY+DHG+AG+LGMPE DYV+  DL+
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFP 240
           + LK+KH+AKSYKSMVIYVEACESGSM EGLLPENIKIYATTASNATE+SWATYCP +  
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 241 S-PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKH 300
             PP DY+TCLGDLYS+AWMED                                  SDKH
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMED----------------------------------SDKH 300

Query: 301 DLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQ 360
           DLSKETL QQY AVRRRTLVDK G GSHVMLYGN+ IG + L+T+IG +PD  NY SSVQ
Sbjct: 301 DLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGNRRIGTDFLNTFIGNSPDYNNYKSSVQ 360

Query: 361 STDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDS 420
           S  TI+ PSK L SN VSQRDASLI+Y +KFQKAP GSREK EAR+QLEDEIL RRH D+
Sbjct: 361 SIHTISSPSK-LSSNGVSQRDASLIYYQYKFQKAPIGSREKMEAREQLEDEILKRRHADN 420

Query: 421 SIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRA 480
           SI +I KLLFGQA+SS+VLN VR+QGQSLVDDW CFK FVK YEKHC+R+S YGMKYTRA
Sbjct: 421 SINNIGKLLFGQARSSKVLNKVREQGQSLVDDWHCFKTFVKIYEKHCKRMSSYGMKYTRA 471

Query: 481 LANICNAGITINQMDQACLETCLVKT 505
           +ANICNAGIT++QMDQACL+TCL KT
Sbjct: 481 IANICNAGITMHQMDQACLQTCLGKT 471

[Close]

BLAST of Moc09g01220 vs. NCBI nr
Match: XP_023516171.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-200
Identity = 347/502 (69.12%), Postives = 402/502 (80.08%), Query Frame = 0


Query: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62
           T +AT+   +LLIALL F+ IIAK++ V  A+ DQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 2   TTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADVC 61

Query: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122
           HAYQILRK G+PDENIIVFMYDDIAF+P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 62  HAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 121

Query: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181

Query: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242
           K+KHEAKSYKSMVIYVEACESGSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 182 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241

Query: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302
           T YDTCLGDLYS+AWMED                                  SDKHD SK
Sbjct: 242 TGYDTCLGDLYSVAWMED----------------------------------SDKHDSSK 301

Query: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362
           ETL QQY  VRRRT VDK GYGSHVMLYGN+SIGNN L+TY G + D+ N  SSV+ST  
Sbjct: 302 ETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIGNNFLNTYFGNSSDDNNNESSVKSTHI 361

Query: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422
           I+       +N VSQRDASL+HYW+KFQ AP+GSREK EA ++LEDEIL+RRH D+SI  
Sbjct: 362 ISS-----LANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQ 421

Query: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482
           I +LLFG+AKSS VLN +R  GQS VDDW CFKK+VK YEKHC+R+SRYGMKYT+ALANI
Sbjct: 422 IGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANI 463

Query: 483 CNAGITINQMDQACLETCLVKT 505
           CNAGIT++QMDQACL+TC+  T
Sbjct: 482 CNAGITMDQMDQACLQTCVGST 463

[Close]

BLAST of Moc09g01220 vs. NCBI nr
Match: XP_022961313.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata])

HSP 1 Score: 707.2 bits (1824), Expect = 1.0e-199
Identity = 345/502 (68.73%), Postives = 401/502 (79.88%), Query Frame = 0


Query: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62
           T +AT+   +LLIALL F+ IIAK++ V  A+TDQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 20  TTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVC 79

Query: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122
           HAYQILRK G+PDENIIVFMYDDIA +P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 80  HAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 139

Query: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 140 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 199

Query: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242
           K+KHEAKSYKSMVIYVEACE+GS+ EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 200 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 259

Query: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302
           T YDTCLGDLYS+AWMED                                  SDKHD SK
Sbjct: 260 TGYDTCLGDLYSVAWMED----------------------------------SDKHDSSK 319

Query: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362
           ETL QQY  VRRRT VDK GYGSHVMLYGN+SIGNN L+TY G   D+ N  SSV+ST  
Sbjct: 320 ETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIGNNFLNTYFGNTSDDNNNESSVKSTHI 379

Query: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422
           I+       SN VSQRDASL+HYW+KFQ AP+GSREK EA ++LEDEIL+RRH D+S+  
Sbjct: 380 ISS-----LSNGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSVNQ 439

Query: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482
           I +LLFG+AKSS VLN +R  GQS VDDW CFKK+VK YEKHC+R+SRYGMKYT+ALANI
Sbjct: 440 IGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANI 481

Query: 483 CNAGITINQMDQACLETCLVKT 505
           CNAGITI+QMD+ACL+TC+  T
Sbjct: 500 CNAGITIDQMDKACLQTCVGNT 481

[Close]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 535.8 bits (1379), Expect = 5.3e-151
Identity = 265/504 (52.58%), Postives = 342/504 (67.86%), Query Frame = 0


Query: 11  FVLLIALLLFS------DIIAKRES--------VDGASTDQPGKRWAILVAGSSGYENYR 70
           FVLL++L+  S      D + K  S         +       G RWA+LVAGSSGY NYR
Sbjct: 15  FVLLVSLVAVSAARSGPDDVIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYR 74

Query: 71  HQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYT 130
           HQAD+CHAYQ+LRKGGL +ENI+VFMYDDIA N  NPRPG +IN P G DVYQGVPKDYT
Sbjct: 75  HQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVYQGVPKDYT 134

Query: 131 GEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAK 190
           G+ VN  N +AVILG+++A+ GGSGKVVDS  +DHIFI+Y+DHG  G+LGMP   Y+YA 
Sbjct: 135 GDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYAN 194

Query: 191 DLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPG 250
           DL +VLK+KH   +YKS+V Y+EACESGS+ EGLLPE + IYATTASNA E+SW TYCPG
Sbjct: 195 DLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPG 254

Query: 251 QFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSD 310
           + PSPP +Y+TCLGDLYS+AWMED                                  S 
Sbjct: 255 EEPSPPPEYETCLGDLYSVAWMED----------------------------------SG 314

Query: 311 KHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSS 370
            H+L  ETL QQY+ V+RRT    + YGSHVM YG+  I  ++LD Y+G NP N N+T +
Sbjct: 315 MHNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA 374

Query: 371 VQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHV 430
               +++ PPS++      +QRDA L+H+W K++KAP GS  KTEA+KQ+ + + +R H+
Sbjct: 375 --DANSLKPPSRV-----TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHI 434

Query: 431 DSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYT 490
           D+S+  + K+LFG ++  EVLN VR  GQ LVDDW C K  V+ +E+HC  LS+YG+K+ 
Sbjct: 435 DNSVILVGKILFGISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHM 477

Query: 491 RALANICNAGITINQMDQACLETC 501
           R+ ANICNAGI + QM++A  + C
Sbjct: 495 RSFANICNAGIQMEQMEEAASQAC 477

[Close]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 5.8e-150
Identity = 264/465 (56.77%), Postives = 323/465 (69.46%), Query Frame = 0


Query: 36  DQPGKRWAILVAGSSGYENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRP 95
           D  G RWA+L+AGS+G+ NYRHQAD+CHAYQ+LRKGGL DENIIVFMYDDIAFN  NPRP
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 96  GVVINKPDGVDVYQGVPKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIY 155
           GV+IN P G DVY+GVPKDYTGE V    F+AV+LGN++ALTGGSGKVVDS  +DHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 156 YTDHGSAGLLGMPEGDYVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENI 215
           Y+DHG  G+LGMP   Y+YA +L++VLK+KH + +YKS+V Y+EACESGS+ EGLL E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 216 KIYATTASNATENSWATYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKAN 275
            IYATTASNA E+SW TYCPG+ P PP +Y TCLGDLYSIAWMED               
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMED--------------- 294

Query: 276 LYLANPRHIKMNLCCAMICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSI 335
                              SD H+L  ETL QQY+ V+ RT      YGSHVM YG+  +
Sbjct: 295 -------------------SDIHNLRTETLHQQYELVKTRT-ASYNSYGSHVMQYGDIGL 354

Query: 336 GNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFG 395
             N+L TY+G NP N NYT      +++ P SK     AV+QRDA L+H+W K++KAP G
Sbjct: 355 SKNNLFTYLGTNPANDNYT--FVDENSLRPASK-----AVNQRDADLLHFWDKYRKAPEG 414

Query: 396 SREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFK 455
           +  K EA+KQ  + + +R HVD SI  I KLLFG  K  E+LN VR  GQ LVDDWGC K
Sbjct: 415 TPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFGIEKGPEILNTVRPAGQPLVDDWGCLK 474

Query: 456 KFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETC 501
             V+T+E HC  LS+YGMK+ R+LANICN GI   +M +A  + C
Sbjct: 475 SLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQAC 477

[Close]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 270/510 (52.94%), Postives = 339/510 (66.47%), Query Frame = 0


Query: 1   MATCYATSTKFVLLIALLLFSDIIAKRESV---------DGASTDQPGKRWAILVAGSSG 60
           MAT  AT T  + L+ L L + + A R+ V          G   +  G RWAIL AGSSG
Sbjct: 1   MATTTAT-TSLLALLLLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSG 60

Query: 61  YENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGV 120
           Y NYRHQAD+CHAYQ+LRKGGL DENIIVFMYDDIAFN  NPR GV+IN P+G +VY+GV
Sbjct: 61  YWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGV 120

Query: 121 PKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGD 180
           PKDYTGE V + NFYA +LG++S LTGGSGKVV+S  +DHIFI+Y+DHG  G+LG P G 
Sbjct: 121 PKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGP 180

Query: 181 YVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWA 240
           Y+YA DL EVLK+KH + +YK++V Y+EACESGS+ EGLLPE+I +YATTASNA E+SW 
Sbjct: 181 YIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWG 240

Query: 241 TYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCA 300
           TYCPG+ PSPP +Y TCLGDLYS+AWMED                               
Sbjct: 241 TYCPGEDPSPPPEYSTCLGDLYSVAWMED------------------------------- 300

Query: 301 MICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNY 360
              SD+H+L  ETL QQY  V+ RT+     YGSHVM YG+  +  + L  Y+G +P N 
Sbjct: 301 ---SDRHNLRTETLHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDPANE 360

Query: 361 NYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEIL 420
           N T        +   S    S AV+QRDA L+H+W KF+KAP GS +K EARKQ+ + + 
Sbjct: 361 NLT-------FVDENSLWSSSKAVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMS 420

Query: 421 NRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRY 480
           +R H+D S+  + KLLFG  K+ E+LN VR  G +LVDDW C K  V+T+E HC  LS+Y
Sbjct: 421 HRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSALVDDWDCLKTMVRTFETHCGSLSQY 468

Query: 481 GMKYTRALANICNAGITINQMDQACLETCL 502
           GMK+ R+ AN+CN GI   QM +A  + C+
Sbjct: 481 GMKHMRSFANMCNVGIKKEQMREASAQACV 468

[Close]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.7e-147
Identity = 260/467 (55.67%), Postives = 323/467 (69.16%), Query Frame = 0


Query: 36  DQPGKRWAILVAGSSGYENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRP 95
           D  G RWAIL+AGS+GY NYRHQ+DVCHAYQ+LRKGG  +ENIIVFMYDDIA N  NPRP
Sbjct: 51  DFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRP 110

Query: 96  GVVINKPDGVDVYQGVPKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIY 155
           GV+INKPDG DVY GVPKDYTG  V++ NFYA +LGN+SALTGGSGKVVDS  +DHIF+Y
Sbjct: 111 GVIINKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVY 170

Query: 156 YTDHGSAGLLGMPEGDYVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENI 215
           YTDHG  G+LGMP G Y+YA DL EVLK+KH + +YKS+V Y+EACESGS+ EGLLP+++
Sbjct: 171 YTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDL 230

Query: 216 KIYATTASNATENSWATYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKAN 275
            IYATTASNA E+SW  YCPG  P PP +Y TCLGDLYSIAWMED               
Sbjct: 231 NIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMED--------------- 290

Query: 276 LYLANPRHIKMNLCCAMICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSI 335
                              S+ H+L  E+L QQY  V+ RT+ +   YGSHVM YG+  +
Sbjct: 291 -------------------SEVHNLQTESLQQQYKLVKNRTISEP--YGSHVMEYGDIGL 350

Query: 336 GNNSLDTYIGANP--DNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAP 395
             N L  Y+G NP  DN ++    +++  +  PS      AV+QRDA LIH+W KF+KAP
Sbjct: 351 SKNDLYQYLGTNPANDNNSFVDETENSLKLRTPSA-----AVNQRDADLIHFWEKFRKAP 410

Query: 396 FGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGC 455
            GS +K EA KQ+ + + +R+H+D+S+  I +LLFG  K +E+L+ VR  G  LVD+W C
Sbjct: 411 EGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGIEKGTELLDVVRPAGSPLVDNWDC 470

Query: 456 FKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETC 501
            K  VKT+E HC  LS+YGMK+ R+ ANICNAGI    M +A  + C
Sbjct: 471 LKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNEPMAEASAQAC 476

[Close]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 516.9 bits (1330), Expect = 2.5e-145
Identity = 250/465 (53.76%), Postives = 326/465 (70.11%), Query Frame = 0


Query: 36  DQPGKRWAILVAGSSGYENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRP 95
           D    +WA+LVAGSSGY NYRHQADVCHAYQ+L+KGG+ +ENI+VFMYDDIA N  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 96  GVVINKPDGVDVYQGVPKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIY 155
           GV+IN P+G DVY GVPKDYTG+ VN  N  AVILGN++AL GGSGKVVDS  +DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 156 YTDHGSAGLLGMPEGDYVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENI 215
           Y+DHG  G+LGMP    +YA DL +VLK+K+ + +YKS+V Y+EACESGS+ EGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 216 KIYATTASNATENSWATYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKAN 275
            IYATTASNA E+SW TYCPG+ PSPP++Y+TCLGDLYS+AW+ED               
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIED--------------- 278

Query: 276 LYLANPRHIKMNLCCAMICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSI 335
                              S+KH+L  ETL +QY+ V++RT      YGSHVM +G+  +
Sbjct: 279 -------------------SEKHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGL 338

Query: 336 GNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFG 395
               L  ++G NP + N+T    + ++I PPS++      +QRDA L+H+WHK+QKAP G
Sbjct: 339 SKEKLVLFMGTNPADENFT--FVNENSIRPPSRV-----TNQRDADLVHFWHKYQKAPEG 398

Query: 396 SREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFK 455
           S  K EA+KQ+ + + +R HVD+SI  I  LLFG  +   VLN VR  G+ LVDDW C K
Sbjct: 399 SARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGHAVLNKVRPSGEPLVDDWDCLK 458

Query: 456 KFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETC 501
             V+ +E+HC  LS+YG+K+ R++AN+CNAGI + QM++A ++ C
Sbjct: 459 SLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEEAAMQAC 461

[Close]

BLAST of Moc09g01220 vs. ExPASy TrEMBL
Match: A0A6J1D2Z4 (vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.2e-299
Identity = 504/504 (100.00%), Postives = 504/504 (100.00%), Query Frame = 0


Query: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60
           MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120
           VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180
           NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240
           VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300
           PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300

Query: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360
           SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420
           DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480
           YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480

Query: 481 NICNAGITINQMDQACLETCLVKT 505
           NICNAGITINQMDQACLETCLVKT
Sbjct: 481 NICNAGITINQMDQACLETCLVKT 504

[Close]

BLAST of Moc09g01220 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.8e-272
Identity = 470/504 (93.25%), Postives = 470/504 (93.25%), Query Frame = 0


Query: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60
           MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD
Sbjct: 1   MATCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQAD 60

Query: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120
           VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV
Sbjct: 61  VCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHV 120

Query: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180
           NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME
Sbjct: 121 NSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLME 180

Query: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240
           VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS
Sbjct: 181 VLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPS 240

Query: 241 PPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDL 300
           PPTDYDTCLGDLYSIAWMED                                  SDKHDL
Sbjct: 241 PPTDYDTCLGDLYSIAWMED----------------------------------SDKHDL 300

Query: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360
           SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST
Sbjct: 301 SKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQST 360

Query: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420
           DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI
Sbjct: 361 DTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSI 420

Query: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 480
           YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA
Sbjct: 421 YHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALA 470

Query: 481 NICNAGITINQMDQACLETCLVKT 505
           NICNAGITINQMDQACLETCLVKT
Sbjct: 481 NICNAGITINQMDQACLETCLVKT 470

[Close]

BLAST of Moc09g01220 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.9e-200
Identity = 345/502 (68.73%), Postives = 401/502 (79.88%), Query Frame = 0


Query: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62
           T +AT+   +LLIALL F+ IIAK++ V  A+TDQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 20  TTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVC 79

Query: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122
           HAYQILRK G+PDENIIVFMYDDIA +P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 80  HAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 139

Query: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 140 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 199

Query: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242
           K+KHEAKSYKSMVIYVEACE+GS+ EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 200 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 259

Query: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302
           T YDTCLGDLYS+AWMED                                  SDKHD SK
Sbjct: 260 TGYDTCLGDLYSVAWMED----------------------------------SDKHDSSK 319

Query: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362
           ETL QQY  VRRRT VDK GYGSHVMLYGN+SIGNN L+TY G   D+ N  SSV+ST  
Sbjct: 320 ETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIGNNFLNTYFGNTSDDNNNESSVKSTHI 379

Query: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422
           I+       SN VSQRDASL+HYW+KFQ AP+GSREK EA ++LEDEIL+RRH D+S+  
Sbjct: 380 ISS-----LSNGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSVNQ 439

Query: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482
           I +LLFG+AKSS VLN +R  GQS VDDW CFKK+VK YEKHC+R+SRYGMKYT+ALANI
Sbjct: 440 IGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANI 481

Query: 483 CNAGITINQMDQACLETCLVKT 505
           CNAGITI+QMD+ACL+TC+  T
Sbjct: 500 CNAGITIDQMDKACLQTCVGNT 481

[Close]

BLAST of Moc09g01220 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 2.0e-198
Identity = 346/502 (68.92%), Postives = 395/502 (78.69%), Query Frame = 0


Query: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62
           T +AT+   +LLI LL F+ IIAK+E V  A+TDQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 2   TTFATTPTTLLLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVC 61

Query: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122
           HAYQILRK G+PDENIIVFMYDDIAF+P NPRPG++INKP G DVY GVPKDYTG+ VNS
Sbjct: 62  HAYQILRK-GIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDVNS 121

Query: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181

Query: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242
           K+KHEAKSYKSMVIYVEACESGSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 182 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241

Query: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302
           T YDTCLGDLYS+AWMED                                  SDKHD SK
Sbjct: 242 TGYDTCLGDLYSVAWMED----------------------------------SDKHDSSK 301

Query: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362
           ETL QQY  VRRRT VDK GYGSHVMLYGN+S GNN L+TY G   D+ N  SSV+ST  
Sbjct: 302 ETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESFGNNFLNTYFGNTSDDNNNESSVKSTHI 361

Query: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422
           I+       SNAVSQRDASL+HYW+KFQ AP+GSREK EA +QLEDEIL+RRH D+SI  
Sbjct: 362 ISS-----LSNAVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQ 421

Query: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482
           I  LLFG+AKSS VL   R  GQS VDDW CFK +VK YEKHC+R+SRYGMKYT+ALAN 
Sbjct: 422 IGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCFKNYVKIYEKHCKRMSRYGMKYTQALANT 463

Query: 483 CNAGITINQMDQACLETCLVKT 505
           CNAGIT++QMDQACL+TC+  T
Sbjct: 482 CNAGITMDQMDQACLQTCVGNT 463

[Close]

BLAST of Moc09g01220 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 1.2e-193
Identity = 343/505 (67.92%), Postives = 395/505 (78.22%), Query Frame = 0


Query: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60
           M TC    TK  ++LI  L F++IIAK E +  A+TDQ GK WA+L+AGS+GY+NYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120
                      GG+PDENIIVFMYDDIAF+PSNPRPG++INKPDG DVY GVPKDYTG+H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180
           VN++NFYA ILGN+SALTGGSGKV+ S   DHIFIYY+DHG+AG+LGMPEGDY++  DL+
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFP 240
           +VLK+KH+AK+YKSMVIYVEACESGSM EG+LPENIKIYATTASNATE+SWATYCP Q  
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 SPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHD 300
             P DY+TCLGDL+S+AWMED                                  SDKHD
Sbjct: 241 RSPKDYNTCLGDLFSVAWMED----------------------------------SDKHD 300

Query: 301 LSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQS 360
           LSKETL QQY AVRRRT VDK GYGSHVMLYGNK IGN+ LDT++G +PD  NY  SVQS
Sbjct: 301 LSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQS 360

Query: 361 TDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSS 420
             TI+  SK L SN+VSQRDASLIHY +KFQ+APFGSREK EAR+QLEDEI  RRH D+S
Sbjct: 361 PQTISSLSK-LSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNS 420

Query: 421 IYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRAL 480
           I  I KLLFGQA+SSE+L+NVR QGQSLVDDW CFK FVK YEKHC R+S YGMKYTRA+
Sbjct: 421 INSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAI 458

Query: 481 ANICNAGITINQMDQACLETCLVKT 505
           ANICNAGIT++QMDQACL+TCL KT
Sbjct: 481 ANICNAGITMHQMDQACLQTCLGKT 458

[Close]

BLAST of Moc09g01220 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 535.8 bits (1379), Expect = 3.7e-152
Identity = 265/504 (52.58%), Postives = 342/504 (67.86%), Query Frame = 0


Query: 11  FVLLIALLLFS------DIIAKRES--------VDGASTDQPGKRWAILVAGSSGYENYR 70
           FVLL++L+  S      D + K  S         +       G RWA+LVAGSSGY NYR
Sbjct: 15  FVLLVSLVAVSAARSGPDDVIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYR 74

Query: 71  HQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYT 130
           HQAD+CHAYQ+LRKGGL +ENI+VFMYDDIA N  NPRPG +IN P G DVYQGVPKDYT
Sbjct: 75  HQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVYQGVPKDYT 134

Query: 131 GEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAK 190
           G+ VN  N +AVILG+++A+ GGSGKVVDS  +DHIFI+Y+DHG  G+LGMP   Y+YA 
Sbjct: 135 GDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYAN 194

Query: 191 DLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPG 250
           DL +VLK+KH   +YKS+V Y+EACESGS+ EGLLPE + IYATTASNA E+SW TYCPG
Sbjct: 195 DLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPG 254

Query: 251 QFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSD 310
           + PSPP +Y+TCLGDLYS+AWMED                                  S 
Sbjct: 255 EEPSPPPEYETCLGDLYSVAWMED----------------------------------SG 314

Query: 311 KHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSS 370
            H+L  ETL QQY+ V+RRT    + YGSHVM YG+  I  ++LD Y+G NP N N+T +
Sbjct: 315 MHNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA 374

Query: 371 VQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHV 430
               +++ PPS++      +QRDA L+H+W K++KAP GS  KTEA+KQ+ + + +R H+
Sbjct: 375 --DANSLKPPSRV-----TNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHI 434

Query: 431 DSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYT 490
           D+S+  + K+LFG ++  EVLN VR  GQ LVDDW C K  V+ +E+HC  LS+YG+K+ 
Sbjct: 435 DNSVILVGKILFGISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHM 477

Query: 491 RALANICNAGITINQMDQACLETC 501
           R+ ANICNAGI + QM++A  + C
Sbjct: 495 RSFANICNAGIQMEQMEEAASQAC 477

[Close]

BLAST of Moc09g01220 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 516.9 bits (1330), Expect = 1.8e-146
Identity = 250/465 (53.76%), Postives = 326/465 (70.11%), Query Frame = 0


Query: 36  DQPGKRWAILVAGSSGYENYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRP 95
           D    +WA+LVAGSSGY NYRHQADVCHAYQ+L+KGG+ +ENI+VFMYDDIA N  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 96  GVVINKPDGVDVYQGVPKDYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIY 155
           GV+IN P+G DVY GVPKDYTG+ VN  N  AVILGN++AL GGSGKVVDS  +DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 156 YTDHGSAGLLGMPEGDYVYAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENI 215
           Y+DHG  G+LGMP    +YA DL +VLK+K+ + +YKS+V Y+EACESGS+ EGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 216 KIYATTASNATENSWATYCPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKAN 275
            IYATTASNA E+SW TYCPG+ PSPP++Y+TCLGDLYS+AW+ED               
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIED--------------- 278

Query: 276 LYLANPRHIKMNLCCAMICSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSI 335
                              S+KH+L  ETL +QY+ V++RT      YGSHVM +G+  +
Sbjct: 279 -------------------SEKHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGL 338

Query: 336 GNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFG 395
               L  ++G NP + N+T    + ++I PPS++      +QRDA L+H+WHK+QKAP G
Sbjct: 339 SKEKLVLFMGTNPADENFT--FVNENSIRPPSRV-----TNQRDADLVHFWHKYQKAPEG 398

Query: 396 SREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFK 455
           S  K EA+KQ+ + + +R HVD+SI  I  LLFG  +   VLN VR  G+ LVDDW C K
Sbjct: 399 SARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGHAVLNKVRPSGEPLVDDWDCLK 458

Query: 456 KFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETC 501
             V+ +E+HC  LS+YG+K+ R++AN+CNAGI + QM++A ++ C
Sbjct: 459 SLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEEAAMQAC 461

[Close]

BLAST of Moc09g01220 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 474.2 bits (1219), Expect = 1.3e-133
Identity = 245/508 (48.23%), Postives = 322/508 (63.39%), Query Frame = 0


Query: 3   TCYATSTKFVLLIALL-------LFSDIIAKRESVDGASTDQP--GKRWAILVAGSSGYE 62
           +CY      +LL+ L+           I+   E  + A  D+   G RWA+LVAGSSGY 
Sbjct: 4   SCYFRPALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYG 63

Query: 63  NYRHQADVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPK 122
           NYRHQADVCHAYQILRKGGL +ENI+V MYDDIA +P NPRPG +IN PDG DVY GVPK
Sbjct: 64  NYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYAGVPK 123

Query: 123 DYTGEHVNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYV 182
           DYTG  V + NFYAV+LG++ A+ GGSGKV+ S  +DHIF+YY DHG  G+LGMP   ++
Sbjct: 124 DYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHI 183

Query: 183 YAKDLMEVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATY 242
           YA D +E LK+KH + +YK MVIYVEACESGS+ EG++P+++ IY TTASNA E+S+ TY
Sbjct: 184 YAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTY 243

Query: 243 CPGQFPSPPTDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMI 302
           CPG  PSPP++Y TCLGDLYS+AWMED                                 
Sbjct: 244 CPGMNPSPPSEYITCLGDLYSVAWMED--------------------------------- 303

Query: 303 CSDKHDLSKETLIQQYDAVRRRTL-VDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYN 362
            S+ H+L KET+ QQY  V+ RT   + +  GSHVM YGN SI +  L  Y G +P   N
Sbjct: 304 -SETHNLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVN 363

Query: 363 YTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILN 422
                   + +   SK+     V+QRDA L+  WH ++ +  GSR+K +  K+L +   +
Sbjct: 364 L-----PLNELPVKSKI---GVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRH 423

Query: 423 RRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYG 482
           R+H+D+S+  IA +LFG   +  VLN VR+ G  LVDDW C K  V+ +E+HC  L++YG
Sbjct: 424 RKHLDASVELIATILFG--PTMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYG 467

Query: 483 MKYTRALANICNAGITINQMDQACLETC 501
           MK+ RA AN+CN G++   M++A    C
Sbjct: 484 MKHMRAFANVCNNGVSKELMEEASTAAC 467

[Close]

BLAST of Moc09g01220 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 472.6 bits (1215), Expect = 3.9e-133
Identity = 249/494 (50.40%), Postives = 314/494 (63.56%), Query Frame = 0


Query: 12  VLLIALLLFSDIIAKRE-----SVDGASTDQPGKRWAILVAGSSGYENYRHQADVCHAYQ 71
           V L ALL+FS    K +      V+ +     G RWA+LVAGS+ Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 72  ILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNSINFY 131
           ILRKGGL DENIIVFMYDDIAF+  NPRPGV+INKPDG DVY+GVPKDYT E VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 132 AVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVLKQKH 191
            V+LGN S +TGG+GKVV S  +D+IFIYY DHG+ GL+ MP GD V AKD  EVL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 192 EAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPPTDYD 251
           + K Y  MVIYVEACESGSM EG+L +N+ IYA TA+N+ E+SW  YCP  +P PP++  
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 252 TCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSKETLI 311
           TCLGD +SI+W+ED                                  SD HD+SKETL 
Sbjct: 252 TCLGDTFSISWLED----------------------------------SDLHDMSKETLE 311

Query: 312 QQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPP 371
           QQY  V+RR   D     SHV  +G + +  + L +YIG NP+N N+T     T++ + P
Sbjct: 312 QQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDYLSSYIGRNPENDNFT----FTESFSSP 371

Query: 372 SKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYHIAKL 431
             +  S  V+ RD  L++   K QKAP GS E  EA+K+L DE  +R+ +D SI  I +L
Sbjct: 372 --ISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKLLDEKNHRKQIDQSITDILRL 431

Query: 432 LFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANICNAG 491
              Q     +L + R  GQ LVDDW CFK  V +++ HC     YG+KYT ALANICN G
Sbjct: 432 SVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCGATVHYGLKYTGALANICNMG 464

Query: 492 I----TINQMDQAC 497
           +    T++ ++QAC
Sbjct: 492 VDVKQTVSAIEQAC 464

[Close]

BLAST of Moc09g01220 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 469.9 bits (1208), Expect = 2.5e-132
Identity = 246/485 (50.72%), Postives = 308/485 (63.51%), Query Frame = 0


Query: 12  VLLIALLLFSDIIAKRE-----SVDGASTDQPGKRWAILVAGSSGYENYRHQADVCHAYQ 71
           V L ALL+FS    K +      V+ +     G RWA+LVAGS+ Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 72  ILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNSINFY 131
           ILRKGGL DENIIVFMYDDIAF+  NPRPGV+INKPDG DVY+GVPKDYT E VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 132 AVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVLKQKH 191
            V+LGN S +TGG+GKVV S  +D+IFIYY DHG+ GL+ MP GD V AKD  EVL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 192 EAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPPTDYD 251
           + K Y  MVIYVEACESGSM EG+L +N+ IYA TA+N+ E+SW  YCP  +P PP++  
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 252 TCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSKETLI 311
           TCLGD +SI+W+ED                                  SD HD+SKETL 
Sbjct: 252 TCLGDTFSISWLED----------------------------------SDLHDMSKETLE 311

Query: 312 QQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPP 371
           QQY  V+RR   D     SHV  +G + +  + L +YIG NP+N N+T     T++ + P
Sbjct: 312 QQYHVVKRRVGSD-VPETSHVCRFGTEKMLKDYLSSYIGRNPENDNFT----FTESFSSP 371

Query: 372 SKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYHIAKL 431
             +  S  V+ RD  L++   K QKAP GS E  EA+K+L DE  +R+ +D SI  I +L
Sbjct: 372 --ISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKLLDEKNHRKQIDQSITDILRL 431

Query: 432 LFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANICNAG 491
              Q     +L + R  GQ LVDDW CFK  V +++ HC     YG+KYT ALANICN G
Sbjct: 432 SVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCGATVHYGLKYTGALANICNMG 455

The following BLAST results are available for this feature:

BLAST of Moc09g01220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148043.1	2.5e-299	100.00	vacuolar-processing enzyme-like isoform X1 [Momordica charantia]	[more]
XP_022148051.1	5.8e-272	93.25	vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia]	[more]
XP_038880044.1	1.2e-200	69.76	vacuolar-processing enzyme-like [Benincasa hispida]	[more]
XP_023516171.1	1.5e-200	69.12	vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo]	[more]
XP_022961313.1	1.0e-199	68.73	vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata]	[more]

BLAST of Moc09g01220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39119	5.3e-151	52.58	Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]
P49043	5.8e-150	56.77	Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1	[more]
O24325	1.4e-148	52.94	Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1	[more]
P49044	2.7e-147	55.67	Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1	[more]
P49047	2.5e-145	53.76	Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g...	[more]

BLAST of Moc09g01220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2Z4	1.2e-299	100.00	vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1D475	2.8e-272	93.25	vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ...	[more]
A0A6J1HBF5	4.9e-200	68.73	vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JF37	2.0e-198	68.92	vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5A7TXA7	1.2e-193	67.92	Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Moc09g01220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32940.1	3.7e-152	52.58	gamma vacuolar processing enzyme	[more]
AT2G25940.1	1.8e-146	53.76	alpha-vacuolar processing enzyme	[more]
AT1G62710.1	1.3e-133	48.23	beta vacuolar processing enzyme	[more]
AT3G20210.1	3.9e-133	50.40	delta vacuolar processing enzyme	[more]
AT3G20210.2	2.5e-132	50.72	delta vacuolar processing enzyme	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001096	Peptidase C13, legumain	PRINTS	PR00776	HEMOGLOBNASE	coord: 114..129 score: 55.43 coord: 142..161 score: 57.61 coord: 191..207 score: 62.92 coord: 42..66 score: 74.78 coord: 67..96 score: 63.48
IPR001096	Peptidase C13, legumain	PIRSF	PIRSF019663	Legumain	coord: 289..504 e-value: 8.3E-62 score: 207.5 coord: 3..266 e-value: 2.3E-138 score: 460.0
IPR001096	Peptidase C13, legumain	PFAM	PF01650	Peptidase_C13	coord: 41..262 e-value: 5.6E-97 score: 324.3
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 23..262
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 294..502
None	No IPR available	GENE3D	3.40.50.1460		coord: 39..367 e-value: 1.9E-122 score: 410.2
None	No IPR available	GENE3D	1.10.132.130		coord: 377..504 e-value: 4.7E-37 score: 128.9
None	No IPR available	PIRSR	PIRSR019663-1	PIRSR019663-1	coord: 39..262 e-value: 4.0E-132 score: 439.4
None	No IPR available	PANTHER	PTHR12000:SF45	VACUOLAR-PROCESSING ENZYME-LIKE	coord: 294..502 coord: 23..262
IPR043577	Asparaginyl endopeptidase	PIRSF	PIRSF500139	AE	coord: 1..265 e-value: 1.9E-138 score: 460.4 coord: 289..504 e-value: 4.6E-62 score: 208.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g01220.1	Moc09g01220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006624	vacuolar protein processing
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008233	peptidase activity