Moc08g10400 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g10400
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPlant protein of unknown function (DUF247)
Locationchr8: 7712056 .. 7713708 (+)
RNA-Seq ExpressionMoc08g10400
SyntenyMoc08g10400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCAATTCTTCCTCATCCAACTTCGACGAGATCAGGTGGGTAGTTCGAATCCGACGGACCCTCGATGAAGAACTCGAGGACGACGATGGGATTCCGGTAGCAATTTTCAAGGTCCCCAGATCGTTAATGGCGTCCGATCCAGATTCCTACACTCCGCAGTTGGTCGCTTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCATGAGATGGAGAGATTCAAGCTCTCTGCCGCAAAAAGAGCCAAAAGATTCAGCCCTAGAGACCTCCAGTTTCACGATCTCGTCGAACAACTGACAAGGCTCGAGCAGAAGATTCGAGCGTCTTATCACAGTTACTTGGATTTTAATGGCGAAACTTTGGCCTGGATGATGGCCATTGACGCATCCTTCTTGCTCGAGTTTCTTCATATCTGCGCCGTCCAACTGGGGAAAATGAAGATTCTCCGGCGAGTGTCCTCCAGAATGTCGCATTTGGTCGATTACGCCGGGACGAAGCCGGCGCATAACGCCGTTCTGGGAGACATAATAATGCTAGAAAATCAGATCCCGTTGTTCGTATTGAGGAAGATGATGGAATTTCAATCTGAATCGTCGGAAATCGCCGATGAGATTTTGCTCTCCATGTTAATCGGATTGTTCAGAGAGGTTTCTCCTTTCAGTAATCTCACGGAGGACGTTCCAGAGATTCAAATTTCAGATTGCGCTCATTTGCTGGACTTTTTATACCAAATGACCACGCCCAAATCGATAGAATCATCAGAAATAATCGAAATCCACGAAGAAATCGAACAACCTCCCCAAATTTCAAGTTCCACAAAACGATTCGCACACAGATTGAAGCATTACCTCTCCAAATTAATCAAATCGTTAATTCATTTCACGAAACAAGCGATCCAATCCCAACCTGTGAAGACGGTCGCGCGGCTACCATGGACGATCCTCTCCACCATCCCCGCATTCGCAATCTTAAAACAGCCGGCCGAGATTCTCTTCTTCCGAGATGAAGAAATCCCCAAAGAAACAGAATCTAAAAACCGTAACTCAAATCACAACGCGAACAGACCGCCATTGATCGAGGAAATAACAATCCCTTCCGTCTCGGAACTCTCAAAAGCCGGAGTCCGATTCTCACCCTCCGCCGGCGGAATCTCAACGATCACCTTCGACGCAAAAACCGCAACGCTCCACCTTCCGACGGCGAGGCTGGACGTGAACACACAAGTGACGCTGAGAAATCTAGTGGCGTACGAAGCGTCGAGAGGATCGGGCCCGCTGGTTTTCACTCGGTACACGGAATTGATGAACGGGATCGTGGATTCGGAGGAGGACGTGAGATTGCTGAGAGAAGGAGGGGTGATTCTGAACCATCTGAAGAGCGACGAAGAGGCGGCAGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTTTTGGCATTGGATAAGGTGATCGAAGAAGTGAATGAATATTACGATGGATTATGGAGAGTGAAAATGGGAAGAATTGTGAATGAATATGTTTATAGCTCGTGGCCTTTGCTGGCTCTAATGGCGGCCGCTTGGCTTCTCTTCTTGATCGCTCTGCAAGTTTTTTGCTCTCTGTTTGGTTGTTTTCGCGCCCTTCATCCCAAGCATTCTTGA

mRNA sequence

ATGGCGTCCAATTCTTCCTCATCCAACTTCGACGAGATCAGGTGGGTAGTTCGAATCCGACGGACCCTCGATGAAGAACTCGAGGACGACGATGGGATTCCGGTAGCAATTTTCAAGGTCCCCAGATCGTTAATGGCGTCCGATCCAGATTCCTACACTCCGCAGTTGGTCGCTTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCATGAGATGGAGAGATTCAAGCTCTCTGCCGCAAAAAGAGCCAAAAGATTCAGCCCTAGAGACCTCCAGTTTCACGATCTCGTCGAACAACTGACAAGGCTCGAGCAGAAGATTCGAGCGTCTTATCACAGTTACTTGGATTTTAATGGCGAAACTTTGGCCTGGATGATGGCCATTGACGCATCCTTCTTGCTCGAGTTTCTTCATATCTGCGCCGTCCAACTGGGGAAAATGAAGATTCTCCGGCGAGTGTCCTCCAGAATGTCGCATTTGGTCGATTACGCCGGGACGAAGCCGGCGCATAACGCCGTTCTGGGAGACATAATAATGCTAGAAAATCAGATCCCGTTGTTCGTATTGAGGAAGATGATGGAATTTCAATCTGAATCGTCGGAAATCGCCGATGAGATTTTGCTCTCCATGTTAATCGGATTGTTCAGAGAGGTTTCTCCTTTCAGTAATCTCACGGAGGACGTTCCAGAGATTCAAATTTCAGATTGCGCTCATTTGCTGGACTTTTTATACCAAATGACCACGCCCAAATCGATAGAATCATCAGAAATAATCGAAATCCACGAAGAAATCGAACAACCTCCCCAAATTTCAAGTTCCACAAAACGATTCGCACACAGATTGAAGCATTACCTCTCCAAATTAATCAAATCGTTAATTCATTTCACGAAACAAGCGATCCAATCCCAACCTGTGAAGACGGTCGCGCGGCTACCATGGACGATCCTCTCCACCATCCCCGCATTCGCAATCTTAAAACAGCCGGCCGAGATTCTCTTCTTCCGAGATGAAGAAATCCCCAAAGAAACAGAATCTAAAAACCGTAACTCAAATCACAACGCGAACAGACCGCCATTGATCGAGGAAATAACAATCCCTTCCGTCTCGGAACTCTCAAAAGCCGGAGTCCGATTCTCACCCTCCGCCGGCGGAATCTCAACGATCACCTTCGACGCAAAAACCGCAACGCTCCACCTTCCGACGGCGAGGCTGGACGTGAACACACAAGTGACGCTGAGAAATCTAGTGGCGTACGAAGCGTCGAGAGGATCGGGCCCGCTGGTTTTCACTCGGTACACGGAATTGATGAACGGGATCGTGGATTCGGAGGAGGACGTGAGATTGCTGAGAGAAGGAGGGGTGATTCTGAACCATCTGAAGAGCGACGAAGAGGCGGCAGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTTTTGGCATTGGATAAGGTGATCGAAGAAGTGAATGAATATTACGATGGATTATGGAGAGTGAAAATGGGAAGAATTGTGAATGAATATGTTTATAGCTCGTGGCCTTTGCTGGCTCTAATGGCGGCCGCTTGGCTTCTCTTCTTGATCGCTCTGCAAGTTTTTTGCTCTCTGTTTGGTTGTTTTCGCGCCCTTCATCCCAAGCATTCTTGA

Coding sequence (CDS)

ATGGCGTCCAATTCTTCCTCATCCAACTTCGACGAGATCAGGTGGGTAGTTCGAATCCGACGGACCCTCGATGAAGAACTCGAGGACGACGATGGGATTCCGGTAGCAATTTTCAAGGTCCCCAGATCGTTAATGGCGTCCGATCCAGATTCCTACACTCCGCAGTTGGTCGCTTTGGGTCCTTACCATTGTTGGCGTCCGGAGCTCCATGAGATGGAGAGATTCAAGCTCTCTGCCGCAAAAAGAGCCAAAAGATTCAGCCCTAGAGACCTCCAGTTTCACGATCTCGTCGAACAACTGACAAGGCTCGAGCAGAAGATTCGAGCGTCTTATCACAGTTACTTGGATTTTAATGGCGAAACTTTGGCCTGGATGATGGCCATTGACGCATCCTTCTTGCTCGAGTTTCTTCATATCTGCGCCGTCCAACTGGGGAAAATGAAGATTCTCCGGCGAGTGTCCTCCAGAATGTCGCATTTGGTCGATTACGCCGGGACGAAGCCGGCGCATAACGCCGTTCTGGGAGACATAATAATGCTAGAAAATCAGATCCCGTTGTTCGTATTGAGGAAGATGATGGAATTTCAATCTGAATCGTCGGAAATCGCCGATGAGATTTTGCTCTCCATGTTAATCGGATTGTTCAGAGAGGTTTCTCCTTTCAGTAATCTCACGGAGGACGTTCCAGAGATTCAAATTTCAGATTGCGCTCATTTGCTGGACTTTTTATACCAAATGACCACGCCCAAATCGATAGAATCATCAGAAATAATCGAAATCCACGAAGAAATCGAACAACCTCCCCAAATTTCAAGTTCCACAAAACGATTCGCACACAGATTGAAGCATTACCTCTCCAAATTAATCAAATCGTTAATTCATTTCACGAAACAAGCGATCCAATCCCAACCTGTGAAGACGGTCGCGCGGCTACCATGGACGATCCTCTCCACCATCCCCGCATTCGCAATCTTAAAACAGCCGGCCGAGATTCTCTTCTTCCGAGATGAAGAAATCCCCAAAGAAACAGAATCTAAAAACCGTAACTCAAATCACAACGCGAACAGACCGCCATTGATCGAGGAAATAACAATCCCTTCCGTCTCGGAACTCTCAAAAGCCGGAGTCCGATTCTCACCCTCCGCCGGCGGAATCTCAACGATCACCTTCGACGCAAAAACCGCAACGCTCCACCTTCCGACGGCGAGGCTGGACGTGAACACACAAGTGACGCTGAGAAATCTAGTGGCGTACGAAGCGTCGAGAGGATCGGGCCCGCTGGTTTTCACTCGGTACACGGAATTGATGAACGGGATCGTGGATTCGGAGGAGGACGTGAGATTGCTGAGAGAAGGAGGGGTGATTCTGAACCATCTGAAGAGCGACGAAGAGGCGGCAGCGATGTGGAATGGGATGAGCAAATCGATCAGATTGAGCAGAGTTTTGGCATTGGATAAGGTGATCGAAGAAGTGAATGAATATTACGATGGATTATGGAGAGTGAAAATGGGAAGAATTGTGAATGAATATGTTTATAGCTCGTGGCCTTTGCTGGCTCTAATGGCGGCCGCTTGGCTTCTCTTCTTGATCGCTCTGCAAGTTTTTTGCTCTCTGTTTGGTTGTTTTCGCGCCCTTCATCCCAAGCATTCTTGA

Protein sequence

MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGETLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAIQSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLIEEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSRVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFGCFRALHPKHS
Homology
BLAST of Moc08g10400 vs. NCBI nr
Match: XP_022153444.1 (putative UPF0481 protein At3g02645 [Momordica charantia])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG
Sbjct: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE
Sbjct: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML
Sbjct: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI
Sbjct: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI
Sbjct: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA
Sbjct: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR
Sbjct: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG
Sbjct: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540

Query: 541 CFRALHPKHS 551
           CFRALHPKHS
Sbjct: 541 CFRALHPKHS 550

BLAST of Moc08g10400 vs. NCBI nr
Match: XP_023528846.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.9 bits (2184), Expect = 2.0e-241
Identity = 440/548 (80.29%), Postives = 476/548 (86.86%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLMASDPDSYTPQLVALG
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMER KLSAAKRAK+FSPRDLQFHDLVEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQVAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V  Y+YSSWPLLALMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLALMAVAWVLFLIALQAFCSLFG 540

Query: 541 CFRALHPK 549
           CFRAL PK
Sbjct: 541 CFRALPPK 540

BLAST of Moc08g10400 vs. NCBI nr
Match: XP_022956256.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 844.7 bits (2181), Expect = 4.4e-241
Identity = 439/548 (80.11%), Postives = 475/548 (86.68%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLM SDPDSYTPQLVALG
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMVSDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMER KLSAAKRAK+FSPRDLQFHDLVEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSDGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V  Y+YSSWPLLALMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLALMAVAWVLFLIALQAFCSLFG 540

Query: 541 CFRALHPK 549
           CFRAL PK
Sbjct: 541 CFRALPPK 540

BLAST of Moc08g10400 vs. NCBI nr
Match: KAG6581946.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 843.2 bits (2177), Expect = 1.3e-240
Identity = 439/548 (80.11%), Postives = 476/548 (86.86%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLMASDPDSYTPQLVALG
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMER KLSAAKRAK+FSPRDLQFHDLVEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V+ Y+YSSWPLLALMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVDAYIYSSWPLLALMAVAWVLFLIALQAFCSLFG 540

Query: 541 CFRALHPK 549
           CFRAL  K
Sbjct: 541 CFRALPHK 540

BLAST of Moc08g10400 vs. NCBI nr
Match: KAG7018367.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.9 bits (2171), Expect = 6.4e-240
Identity = 438/548 (79.93%), Postives = 475/548 (86.68%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLMASDPDSYTPQLVALG
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMER KLSAAK AK+FSPRDLQFHDLVEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWRPELHEMERIKLSAAKSAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V+ Y+YSSWPLLALMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVDAYIYSSWPLLALMAVAWVLFLIALQAFCSLFG 540

Query: 541 CFRALHPK 549
           CFRAL  K
Sbjct: 541 CFRALPHK 540

BLAST of Moc08g10400 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 8.3e-142
Identity = 274/545 (50.28%), Postives = 366/545 (67.16%), Query Frame = 0

Query: 3   SNSSSSNFDEIRWVVRIRRTLDEELEDDD--GIPVAIFKVPRSLMASDPDSYTPQLVALG 62
           S++    FDE RWV+ ++++LD ELE+ D   + V+IF VP++LM S PDSYTP  V++G
Sbjct: 9   SSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIG 68

Query: 63  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 122
           PYHC +PELHEMER+KL  A++  R      +FHDLVE+L  +E KIRA YH Y+ FNGE
Sbjct: 69  PYHCLKPELHEMERYKLMIARKI-RNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGE 128

Query: 123 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 182
           TL W+MA+D+SFL+EFL        K+   R+V + ++ +         HN +L DI+M+
Sbjct: 129 TLLWIMAVDSSFLIEFL--------KIYSFRKVETLINRV--------GHNEILRDIMMI 188

Query: 183 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTED--VPEIQISDCAH 242
           ENQIPLFVLRK +EFQ ES+E AD++LLS+L GL +++SP     +D  + + Q  +C H
Sbjct: 189 ENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNH 248

Query: 243 LLDFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQ 302
           +LDFLYQM  P+ IE  E+ E  EE        +   RF   +KH             K+
Sbjct: 249 ILDFLYQMIVPR-IEEEELEEDDEENRADENGGNRAIRFMDEIKHQF-----------KR 308

Query: 303 AIQSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPP 362
              S+P   + R PW I+S +P F  LK  A+ LF R E     T  ++  S  +  +PP
Sbjct: 309 VFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQES-VSILDIEKPP 368

Query: 363 LIEEITIPSVSELSKAGVRFSPSA-GGISTITFDAKTATLHLPTARLDVNTQVTLRNLVA 422
           L+EE+TIPSVS+L KAGVRF P+A G IST+TFD+ +   +LP   LD+NT+  LRNLVA
Sbjct: 369 LVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVA 428

Query: 423 YEASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIR 482
           YEA+  SGPLVFTRYTEL+NGI+DSEEDVRLLRE GV+++ LKSD+EAA MWNGMSKS+R
Sbjct: 429 YEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVR 488

Query: 483 LSRVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCS 542
           L++V  LDK IE+VN YY G W+VK+GR+V  YVY SW +LA +AA  LL L++LQ+F  
Sbjct: 489 LTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQLFSL 523

BLAST of Moc08g10400 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.1e-11
Identity = 61/236 (25.85%), Postives = 112/236 (47.46%), Query Frame = 0

Query: 37  IFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHEMERFK-------LSAAKRAKRFSPR 96
           IF+VP S +A +P +Y P++V++GPYH     L  +++ K       L  AK+      +
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKK------K 107

Query: 97  DLQFHDLVEQLTRLEQKIRASYHSYLDFNGETLAWMMAIDASFLLEFLHICAVQLGKMKI 156
           D++ + LV+ +  LE KIR SY   L   G  L +MM +D  F+   L +  +  G +++
Sbjct: 108 DVEENVLVKAVVDLEDKIRKSYSEELK-TGHDLMFMMVLDGCFI---LMVFLIMSGNIEL 167

Query: 157 LRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLRKMMEFQSESSEIADEILLS 216
                  +  L+         +++  D+++LENQ+P FVL+ +       S+I     L+
Sbjct: 168 SEDPIFSIPWLL---------SSIQSDLLLLENQVPFFVLQTLY----VGSKIGVSSDLN 227

Query: 217 MLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPKSIESSEIIEIHEEIE 266
            +   F + +P         + +     HLLD + +   P + ES +    H +++
Sbjct: 228 RIAFHFFK-NPIDKEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQ 259

BLAST of Moc08g10400 vs. ExPASy TrEMBL
Match: A0A6J1DJ23 (putative UPF0481 protein At3g02645 OS=Momordica charantia OX=3673 GN=LOC111020951 PE=4 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG
Sbjct: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE
Sbjct: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML
Sbjct: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI
Sbjct: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI
Sbjct: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA
Sbjct: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR
Sbjct: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG
Sbjct: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540

Query: 541 CFRALHPKHS 551
           CFRALHPKHS
Sbjct: 541 CFRALHPKHS 550

BLAST of Moc08g10400 vs. ExPASy TrEMBL
Match: A0A6J1GW24 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111458007 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 2.1e-241
Identity = 439/548 (80.11%), Postives = 475/548 (86.68%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLM SDPDSYTPQLVALG
Sbjct: 1   MASNSFSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMVSDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCWRPELHEMER KLSAAKRAK+FSPRDLQFHDLVEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWRPELHEMERIKLSAAKRAKKFSPRDLQFHDLVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQIAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSDGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V  Y+YSSWPLLALMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLALMAVAWVLFLIALQAFCSLFG 540

Query: 541 CFRALHPK 549
           CFRAL PK
Sbjct: 541 CFRALPPK 540

BLAST of Moc08g10400 vs. ExPASy TrEMBL
Match: A0A6J1IWY7 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111479236 PE=4 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 4.2e-237
Identity = 433/545 (79.45%), Postives = 471/545 (86.42%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDDGIPVAIFKVPRSLMASDPDSYTPQLVALG 60
           MASNS SS FDE  WV++IRRTL+EEL+DDDGIPV+IFKVP+SLMASDPDSYTPQLVALG
Sbjct: 1   MASNSYSSTFDECNWVIQIRRTLEEELDDDDGIPVSIFKVPKSLMASDPDSYTPQLVALG 60

Query: 61  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 120
           PYHCW PELHEMER KLSAAKRAK+FSPRDL+F D+VEQL +LE KIRA YH YLD N E
Sbjct: 61  PYHCWCPELHEMERIKLSAAKRAKKFSPRDLRFDDIVEQLAKLEHKIRAYYHGYLDLNSE 120

Query: 121 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 180
           TLAWMMAIDA FLLEFL++ A QL   KILRRVSSRMSHL+DYAGTKPAHNAVL DIIML
Sbjct: 121 TLAWMMAIDACFLLEFLNVYAQQL---KILRRVSSRMSHLMDYAGTKPAHNAVLRDIIML 180

Query: 181 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLL 240
           ENQIPLFVLRKMMEFQ ES+E +DE LLSMLIGLF E+SPF N T+++PEIQISDCAHLL
Sbjct: 181 ENQIPLFVLRKMMEFQFESTEQSDETLLSMLIGLFAELSPF-NTTDEIPEIQISDCAHLL 240

Query: 241 DFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAI 300
           DFLYQM TPK  E SEI E   EIEQ PQI S  ++ AHR K  L + I++LI FTK+A+
Sbjct: 241 DFLYQMITPKLKEPSEITENPNEIEQSPQILSYRQQVAHRSKQCLLRSIENLIRFTKRAM 300

Query: 301 QSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI 360
           QSQ VKT+ARLPW ILS+IP FAILKQPAEILFFR++    ETE +N +SN NANRPPL 
Sbjct: 301 QSQHVKTIARLPWAILSSIPPFAILKQPAEILFFREQ----ETEPQNPDSNPNANRPPLT 360

Query: 361 EEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEA 420
           EEITIPSVSEL KAG+RFSPS GGISTITFD  T TL+LPT RLDVNTQ TLRNLVAYEA
Sbjct: 361 EEITIPSVSELFKAGIRFSPSHGGISTITFDRNTGTLYLPTVRLDVNTQATLRNLVAYEA 420

Query: 421 SRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLSR 480
           S G GPLV TRYTELMNGIVDS+EDVRLLREGGVILNHLKSDEE AAMWNGMSKS+RLSR
Sbjct: 421 SSGLGPLVLTRYTELMNGIVDSDEDVRLLREGGVILNHLKSDEEGAAMWNGMSKSMRLSR 480

Query: 481 VLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFG 540
           VL LDKVIEEVNEYYDGLWRVKMGR V  Y+YSSWPLL LMA AW+LFLIALQ FCSLFG
Sbjct: 481 VLELDKVIEEVNEYYDGLWRVKMGRFVEAYIYSSWPLLGLMAVAWVLFLIALQAFCSLFG 537

Query: 541 CFRAL 546
           CFRAL
Sbjct: 541 CFRAL 537

BLAST of Moc08g10400 vs. ExPASy TrEMBL
Match: A0A0A0KYJ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006280 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.9e-229
Identity = 421/552 (76.27%), Postives = 468/552 (84.78%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDD--GIPVAIFKVPRSLMASDPDSYTPQLVA 60
           M SN SSS FDE++WV++IRRTL+EELEDD+  GIPVAIFKVP+ LM SDP SYTPQLVA
Sbjct: 1   MVSNYSSSTFDELKWVIQIRRTLEEELEDDEDYGIPVAIFKVPKLLMESDPHSYTPQLVA 60

Query: 61  LGPYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFN 120
           LGPYHCWRPELH+MER KLSAAKRAK+FS  +LQFH+LV+Q++++EQKIRA YH YLD N
Sbjct: 61  LGPYHCWRPELHDMERLKLSAAKRAKKFSSTNLQFHELVQQMSKIEQKIRACYHGYLDIN 120

Query: 121 GETLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDII 180
           GETLAWMMAIDASFLLEFL + A   G+ KILRRVSSRMSHL+D+AGTKPAHNAVL DII
Sbjct: 121 GETLAWMMAIDASFLLEFLLVYA---GEFKILRRVSSRMSHLMDHAGTKPAHNAVLRDII 180

Query: 181 MLENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAH 240
           MLENQIPLFVLRKMMEFQSESS+ +DEILLSM+IGLF E+SPF N T+ +PEIQISDCAH
Sbjct: 181 MLENQIPLFVLRKMMEFQSESSQQSDEILLSMMIGLFTELSPF-NTTDGIPEIQISDCAH 240

Query: 241 LLDFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQ 300
           LLDFLYQM  PKS ESSEI E   EI+Q PQIS       HR K +L    K LI FTK+
Sbjct: 241 LLDFLYQMIAPKSKESSEINETPNEIDQSPQIS-------HRFKQFL----KRLISFTKR 300

Query: 301 AIQSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPP 360
           AIQSQ VKT+ARLPWTILSTIP F +LKQPAEILFFR+++  KE E + +N N NANRPP
Sbjct: 301 AIQSQHVKTIARLPWTILSTIPPFTVLKQPAEILFFREQQEAKE-EPEPQNPNSNANRPP 360

Query: 361 LIEEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAY 420
           L EEI IPSVSELSKAG+ FSPS GGISTI FD++T TL+LPTARLDVNT+ TLRNLVAY
Sbjct: 361 LTEEIAIPSVSELSKAGIHFSPSHGGISTINFDSQTGTLYLPTARLDVNTEATLRNLVAY 420

Query: 421 EASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRL 480
           EAS G G LV TRYTELMNGIVD+EEDVRLLREGGVI+NHLKSDEE A MWNGMSKSIRL
Sbjct: 421 EASSGIGALVLTRYTELMNGIVDTEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRL 480

Query: 481 SRVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSL 540
           SRV+ LDKVIEEVNEYY+GLWRVKMGR V EY+YSSWPL+AL   AW LFLI LQ FCSL
Sbjct: 481 SRVMELDKVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIGLQAFCSL 536

Query: 541 FGCFRALHPKHS 551
           FGCFRAL  K S
Sbjct: 541 FGCFRALPSKRS 536

BLAST of Moc08g10400 vs. ExPASy TrEMBL
Match: A0A5A7TLR9 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001990 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 4.6e-228
Identity = 418/552 (75.72%), Postives = 465/552 (84.24%), Query Frame = 0

Query: 1   MASNSSSSNFDEIRWVVRIRRTLDEELEDDD--GIPVAIFKVPRSLMASDPDSYTPQLVA 60
           M SN SSS FDE++WV++IRRTL+EE EDD+  GIPVAIFKVP+ LM SDP SY PQLVA
Sbjct: 1   MPSNYSSSTFDELKWVIQIRRTLEEEQEDDEDYGIPVAIFKVPKLLMESDPHSYIPQLVA 60

Query: 61  LGPYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFN 120
           LGPYHCWRPELH+M R KLSAAKRAK+FS  +LQFHDLVEQL +LEQ IRA YH YLD N
Sbjct: 61  LGPYHCWRPELHDMVRLKLSAAKRAKKFSSTNLQFHDLVEQLLKLEQNIRACYHGYLDLN 120

Query: 121 GETLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDII 180
           GETLAWMMAIDASFLLEFLH+ A   GK KIL+RV SRMSHL+D+AGTKPAHNAVL DII
Sbjct: 121 GETLAWMMAIDASFLLEFLHVYA---GKFKILQRVPSRMSHLMDHAGTKPAHNAVLRDII 180

Query: 181 MLENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAH 240
           MLENQIPLFVLRKMMEFQ ESS+ +DEILLSM+IGLF E+SPF N T+ +PEIQISDCAH
Sbjct: 181 MLENQIPLFVLRKMMEFQFESSQQSDEILLSMMIGLFTELSPF-NTTDGIPEIQISDCAH 240

Query: 241 LLDFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQ 300
           LLDFLYQM  PKS E+SEI EI  EIEQ PQIS   K+F           +K LI +TK+
Sbjct: 241 LLDFLYQMIAPKSKEASEINEIPNEIEQSPQISYRFKQF-----------LKCLISYTKR 300

Query: 301 AIQSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPP 360
           A+QSQ +KT+ARLPWTILSTIP F +LKQPAEILFFR+++ P+E E + +N   NANRPP
Sbjct: 301 AMQSQHIKTIARLPWTILSTIPPFTVLKQPAEILFFREQQEPRE-EPEPQNLKSNANRPP 360

Query: 361 LIEEITIPSVSELSKAGVRFSPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAY 420
           L EEI IPSVSELSKAG+RFSPS GGISTITF+++T TL+LPTARLDVNT+ TLRNLVAY
Sbjct: 361 LTEEIAIPSVSELSKAGIRFSPSHGGISTITFNSQTGTLYLPTARLDVNTEATLRNLVAY 420

Query: 421 EASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRL 480
           EAS G G LV TRYTELMNGIVDSEEDVRLLREGGVI+NHLKSDEE A MWNGMSKSIRL
Sbjct: 421 EASSGIGALVLTRYTELMNGIVDSEEDVRLLREGGVIVNHLKSDEEGAWMWNGMSKSIRL 480

Query: 481 SRVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSL 540
           SRV+ LDKVIEEVNEYY+GLWRVKMGR V EY+YSSWPL+AL   AW LFLIALQ FCSL
Sbjct: 481 SRVMELDKVIEEVNEYYNGLWRVKMGRFVKEYIYSSWPLIALTTVAWFLFLIALQAFCSL 536

Query: 541 FGCFRALHPKHS 551
           FGCFRAL  K S
Sbjct: 541 FGCFRALPSKRS 536

BLAST of Moc08g10400 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 505.4 bits (1300), Expect = 5.9e-143
Identity = 274/545 (50.28%), Postives = 366/545 (67.16%), Query Frame = 0

Query: 3   SNSSSSNFDEIRWVVRIRRTLDEELEDDD--GIPVAIFKVPRSLMASDPDSYTPQLVALG 62
           S++    FDE RWV+ ++++LD ELE+ D   + V+IF VP++LM S PDSYTP  V++G
Sbjct: 9   SSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIG 68

Query: 63  PYHCWRPELHEMERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGE 122
           PYHC +PELHEMER+KL  A++  R      +FHDLVE+L  +E KIRA YH Y+ FNGE
Sbjct: 69  PYHCLKPELHEMERYKLMIARKI-RNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGE 128

Query: 123 TLAWMMAIDASFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIML 182
           TL W+MA+D+SFL+EFL        K+   R+V + ++ +         HN +L DI+M+
Sbjct: 129 TLLWIMAVDSSFLIEFL--------KIYSFRKVETLINRV--------GHNEILRDIMMI 188

Query: 183 ENQIPLFVLRKMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTED--VPEIQISDCAH 242
           ENQIPLFVLRK +EFQ ES+E AD++LLS+L GL +++SP     +D  + + Q  +C H
Sbjct: 189 ENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNH 248

Query: 243 LLDFLYQMTTPKSIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQ 302
           +LDFLYQM  P+ IE  E+ E  EE        +   RF   +KH             K+
Sbjct: 249 ILDFLYQMIVPR-IEEEELEEDDEENRADENGGNRAIRFMDEIKHQF-----------KR 308

Query: 303 AIQSQPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPP 362
              S+P   + R PW I+S +P F  LK  A+ LF R E     T  ++  S  +  +PP
Sbjct: 309 VFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQES-VSILDIEKPP 368

Query: 363 LIEEITIPSVSELSKAGVRFSPSA-GGISTITFDAKTATLHLPTARLDVNTQVTLRNLVA 422
           L+EE+TIPSVS+L KAGVRF P+A G IST+TFD+ +   +LP   LD+NT+  LRNLVA
Sbjct: 369 LVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVA 428

Query: 423 YEASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIR 482
           YEA+  SGPLVFTRYTEL+NGI+DSEEDVRLLRE GV+++ LKSD+EAA MWNGMSKS+R
Sbjct: 429 YEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVR 488

Query: 483 LSRVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCS 542
           L++V  LDK IE+VN YY G W+VK+GR+V  YVY SW +LA +AA  LL L++LQ+F  
Sbjct: 489 LTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQLFSL 523

BLAST of Moc08g10400 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 114.4 bits (285), Expect = 2.9e-25
Identity = 124/545 (22.75%), Postives = 211/545 (38.72%), Query Frame = 0

Query: 15  WVVRIRRTLDEELEDDDGI---PVAIFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHE 74
           WV+ IR  L++   DDD      + I++VP  L  +D  SY PQ V+LGPYH  +  L  
Sbjct: 90  WVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRP 149

Query: 75  MERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGETLAWMMAIDAS 134
           MER K  A  +  +   + ++ +     +  LE+K RA Y   +  +      M+ +D  
Sbjct: 150 MERHKWRALNKVLKRLKQRIEMY--TNAMRELEEKARACYEGPISLSRNEFTEMLVLDGC 209

Query: 135 FLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLRK 194
           F+LE         G ++    +    +  V     +   +++  D+IMLENQ+PLFVL +
Sbjct: 210 FVLELFR------GTVEGFTEIGYARNDPV--FAMRGLMHSIQRDMIMLENQLPLFVLDR 269

Query: 195 MMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPKS 254
           ++E Q  +                                Q    AH+    +    P  
Sbjct: 270 LLELQLGTQN------------------------------QTGIVAHVAVKFFDPLMPTG 329

Query: 255 IESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSL-----IH----FTKQAIQS 314
                     E + +P Q          +L ++L K + +L     +H    F +  +QS
Sbjct: 330 ----------EALTKPDQ---------SKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQS 389

Query: 315 QPVKTVARLPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLIEE 374
            P                                      T S  +    N       ++
Sbjct: 390 SPT-----------------------------------PNTRSLLKRLTRNTRVVDKRQQ 449

Query: 375 ITIPSVSELSKAGVRFSPSAGGISTITF---DAKTATLHLPTARLDVNTQVTLRNLVAYE 434
             +  V+EL +AGV+F        T  F   + K   L +P   +   T+    NL+A+E
Sbjct: 450 QLVHCVTELREAGVKFRKR----KTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFE 509

Query: 435 ASRGSGPLVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLS 494
                     T Y   M+ +++S EDV  L   G+I + L SD E A ++N + + +   
Sbjct: 510 QCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFD 536

Query: 495 -RVLALDKVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSL 544
            +   L ++  +VN YY+  W V    + ++Y  + W   +  AA  LL L   Q F ++
Sbjct: 570 PKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAV 536

BLAST of Moc08g10400 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 112.8 bits (281), Expect = 8.5e-25
Identity = 128/537 (23.84%), Postives = 215/537 (40.04%), Query Frame = 0

Query: 15  WVVRIRRTLDEELEDDDGI---PVAIFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHE 74
           WV+ I   L++   DDD      + I++VP  L  +D  SY PQ V+LGPYH  +  L  
Sbjct: 80  WVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRS 139

Query: 75  MERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGETLAWMMAIDAS 134
           M+R K  A  R  + + + ++ +  ++ +  LE+K RA Y   L  +      M+ +D  
Sbjct: 140 MDRHKWRAVNRVLKRTNQGIKMY--IDAMRELEEKARACYEGPLSLSSNEFIEMLVLDGC 199

Query: 135 FLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLRK 194
           F+LE          ++   R               + + +++  D++MLENQ+PLFVL +
Sbjct: 200 FVLELFRGAVEGFTELGYARN--------DPVFAMRGSMHSIQRDMVMLENQLPLFVLNR 259

Query: 195 MMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPKS 254
           ++E Q  +                       N T  V ++ I       D L  M T + 
Sbjct: 260 LLELQLGT----------------------RNQTGLVAQLAI----RFFDPL--MPTDEP 319

Query: 255 IESSEIIEIHEEIEQPPQISSSTKRFAHRLK-HYLSKLIKSLIHFTKQAIQSQPVKTVAR 314
           +  S       ++E       S   FA   + H L    +SL+  +      +P   + R
Sbjct: 320 LTKSG----QSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSS-----PKPEPRLTR 379

Query: 315 LPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLIEEITIPSVSE 374
             W                                 +RN+     R     +  I  V+E
Sbjct: 380 KRW---------------------------------SRNTRVADKR----RQQLIHCVTE 439

Query: 375 LSKAGVRFSPSAGGISTITF---DAKTATLHLPTARLDVNTQVTLRNLVAYEASRGSGPL 434
           L +AG++F        T  F     K   L +P   +   T+    NL+A+E        
Sbjct: 440 LKEAGIKFRRR----KTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFEQCHIDSSN 499

Query: 435 VFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRL-SRVLALDK 494
             T Y   M+ ++DS EDV  L   G+I + L SD E A ++N + + +   +    L +
Sbjct: 500 DITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDTEDSYLSR 528

Query: 495 VIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFGCFR 544
           +  EVN YYD  W      + ++Y  + W +++  AA  LL L   Q F +++  ++
Sbjct: 560 LSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFYAVYAYYK 528

BLAST of Moc08g10400 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 111.3 bits (277), Expect = 2.5e-24
Identity = 122/537 (22.72%), Postives = 214/537 (39.85%), Query Frame = 0

Query: 15  WVVRIRRTLDEELEDD---DGIPVAIFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHE 74
           WV+ I+  +++ L  D       + I++VP  L  +D  SY PQ V++GPYH  +  L  
Sbjct: 67  WVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLRP 126

Query: 75  MERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDF-NGETLAWMMAIDA 134
           MER K  A       +  +++ +  ++ +  LE++ RA Y   +D  N      M+ +D 
Sbjct: 127 MERHKWRAVNMIMARTKHNIEMY--IDAMKELEEEARACYQGPIDMKNSNEFTEMLVLDG 186

Query: 135 SFLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLR 194
            F+LE         G ++  +++    +  V     +   +++  D+IMLENQ+PLFVL 
Sbjct: 187 CFVLELFK------GTIQGFQKIGYARNDPV--FAKRGLMHSIQRDMIMLENQLPLFVLD 246

Query: 195 KMMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPK 254
           +++  Q+ +      I+  + +  F+ + P S +                         K
Sbjct: 247 RLLGLQTGTPN-QTGIVAEVAVRFFKTLMPTSEV-----------------------LTK 306

Query: 255 SIESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAIQSQPVKTVAR 314
           S  S +  E  +E+     +            H L    +SLI  ++   Q  P + ++ 
Sbjct: 307 SERSLDSQEKSDELGDNGGL------------HCLDVFHRSLIQSSETTNQGTPYEDMSM 366

Query: 315 LPWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLIEEITIPSVSE 374
                                       + K+ +                    I  V+E
Sbjct: 367 ----------------------------VEKQQQ-------------------LIHCVTE 426

Query: 375 LSKAGVRF-SPSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEASRGSGPLVF 434
           L  AGV F     G +  I F  K   L +P   +   T+    NL+A+E          
Sbjct: 427 LRGAGVNFMRKETGQLWDIEF--KNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNNI 486

Query: 435 TRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLS-RVLALDKVI 494
           T Y   M+ +++S +DV  L   G+I + L SD E A ++N + K +    +   L ++ 
Sbjct: 487 TSYIIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVADLFNRLCKEVIFDPKDGYLSQLS 508

Query: 495 EEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFGCFRAL 546
            EVN YY   W      +  +Y  + W   +  AA  LLFL   Q F +++  ++ L
Sbjct: 547 REVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAYYKPL 508

BLAST of Moc08g10400 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 109.8 bits (273), Expect = 7.2e-24
Identity = 117/538 (21.75%), Postives = 217/538 (40.33%), Query Frame = 0

Query: 15  WVVRIRRTLDEELED---DDGIPVAIFKVPRSLMASDPDSYTPQLVALGPYHCWRPELHE 74
           WV+ I+  +++ + D        + I++VP SL  SD +SY PQ V+LGPYH     L  
Sbjct: 90  WVIWIKDKMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLRP 149

Query: 75  MERFKLSAAKRAKRFSPRDLQFHDLVEQLTRLEQKIRASYHSYLDFNGETLAWMMAIDAS 134
           M+  K  A     + + + ++ +  ++ +  LE++ RA Y   +  +      M+ +D  
Sbjct: 150 MDYHKWRAVNMVMKRTKQGIEMY--IDAMKELEERARACYEGPIGLSSNKFTQMLVLDGC 209

Query: 135 FLLEFLHICAVQLGKMKILRRVSSRMSHLVDYAGTKPAHNAVLGDIIMLENQIPLFVLRK 194
           F+L+          K+   R               + + +++  D++MLENQ+PLFVL +
Sbjct: 210 FVLDLFRGAYEGFSKLGYDRN--------DPVFAMRGSMHSIRRDMLMLENQLPLFVLNR 269

Query: 195 MMEFQSESSEIADEILLSMLIGLFREVSPFSNLTEDVPEIQISDCAHLLDFLYQMTTPKS 254
           ++E Q   ++    ++  + +  F  + P                       Y  +T   
Sbjct: 270 LLELQL-GTQYQTGLVAQLAVRFFNPLMP----------------------TYMSST--K 329

Query: 255 IESSEIIEIHEEIEQPPQISSSTKRFAHRLKHYLSKLIKSLIHFTKQAIQSQPVKTVARL 314
           IE+S+  E + +   P  I+   K   H L  +   L++  +         +P   ++R 
Sbjct: 330 IENSQ--ENNNKFFNP--IADKEKEELHCLDVFRRSLLQPSL---------KPDPRLSRS 389

Query: 315 PWTILSTIPAFAILKQPAEILFFRDEEIPKETESKNRNSNHNANRPPLI----EEITIPS 374
            W                                         +R PL+    ++  +  
Sbjct: 390 RW-----------------------------------------SRKPLVADKRQQQLLHC 449

Query: 375 VSELSKAGVRFS-PSAGGISTITFDAKTATLHLPTARLDVNTQVTLRNLVAYEASRGSGP 434
           V+EL +AG++F    +     I F  K   L +P   +   T+    NL+AYE       
Sbjct: 450 VTELREAGIKFKRRKSDRFWDIQF--KNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDST 509

Query: 435 LVFTRYTELMNGIVDSEEDVRLLREGGVILNHLKSDEEAAAMWNGMSKSIRLS-RVLALD 494
              T Y   M+ ++DS ED+R L    +I + L +D E A ++N + + +        L 
Sbjct: 510 NDITSYIIFMDNLIDSAEDIRYLHYYDIIEHWLGNDSEVADVFNRLCQEVAFDLENTYLS 536

Query: 495 KVIEEVNEYYDGLWRVKMGRIVNEYVYSSWPLLALMAAAWLLFLIALQVFCSLFGCFR 544
           ++  +V+ YY+  W V    + ++Y  + W   +  AA  LL L   Q F + +  F+
Sbjct: 570 ELSNKVDRYYNRKWNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFTSYPYFK 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153444.10.0e+00100.00putative UPF0481 protein At3g02645 [Momordica charantia][more]
XP_023528846.12.0e-24180.29putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo][more]
XP_022956256.14.4e-24180.11putative UPF0481 protein At3g02645 [Cucurbita moschata][more]
KAG6581946.11.3e-24080.11putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7018367.16.4e-24079.93putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
P0C8978.3e-14250.28Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Q9SD533.1e-1125.85UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DJ230.0e+00100.00putative UPF0481 protein At3g02645 OS=Momordica charantia OX=3673 GN=LOC11102095... [more]
A0A6J1GW242.1e-24180.11putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111458007... [more]
A0A6J1IWY74.2e-23779.45putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111479236 P... [more]
A0A0A0KYJ31.9e-22976.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G006280 PE=4 SV=1[more]
A0A5A7TLR94.6e-22875.72Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Match NameE-valueIdentityDescription
AT3G02645.15.9e-14350.28Plant protein of unknown function (DUF247) [more]
AT3G50170.12.9e-2522.75Plant protein of unknown function (DUF247) [more]
AT3G50120.18.5e-2523.84Plant protein of unknown function (DUF247) [more]
AT3G50150.12.5e-2422.72Plant protein of unknown function (DUF247) [more]
AT3G50140.17.2e-2421.75Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 37..529
e-value: 6.9E-101
score: 338.4
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 3..541
NoneNo IPR availablePANTHERPTHR31549:SF176UPF0481 PLANT-LIKE PROTEINcoord: 3..541

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g10400.1Moc08g10400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane