Moc06g07730 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g07730
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionsubtilisin-like protease SBT6.1
Locationchr6: 5618089 .. 5623682 (-)
RNA-Seq ExpressionMoc06g07730
SyntenyMoc06g07730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAGTTCTTCGGTTTCTTCTTCATATGTCTCCTTCGTCCTCATCTTCGTTCCCATCTTTATCTCTATCTCTCTCTTCCAATTCAAACCCTCTTCCGATCCTATTGTATATCAAACCCTAACCCGAAACTACTCGTCGGTCGATCCATTTACCTCCAAGAACCTCAGTGACGGGAACCAAGTCTCGCGGAAGAACTACATTGTTCGCTTCGTCCAATACAGGGAGGCGAAGGAGCACCGGTCCTATCTGGAATCGCGTATTAGATCGGGTCTCTGGGAATGGATCGAGAGGAAGAACCCTGCGTCTAAGTATCCAACCGAGTTTGGCTTGGTTTCTATTGAGGACTCGGCGAGAGGGGAGTTGATTAAGGATATTGAGGAATTAGAGCTCGTCAAGGATGTGAATGTGGATGCAAGCTACGTGAGGGACGTGCTAACGGAGAATGGGGGTAGAGTCGGGGCTTTTGTAGACGGGAAGAAGCGTCCGGGGAAGATTTTCACCTCCATGTCGTTTAAGGAAGGAGGCGGGGAACGTTATACTGCAATTAGCAATGCCTCTAATAGATGGGGACGGCATCTGTCGATGCAGGTAAATTTCCTTTCAATGACCGATTTATTTTTTCTTTTGATATATTTGGCTTTGGACAATTGACTATGAGGAAAAGGGGGCTGCAGGGGCGACTTCCGGTGTCTCATATAATGAAATTGGATTTATCATATGTAGAGCTCTTGTTCAGTAAATTGATTTATGTATTATTTATTTTTAATGCAACACAGAATTAGCCATTTCAATGTTATAAGCTAAATTTCAATCGAAACTACTGTGATGCATATCTTCTTGTATGCATGTTGAATATTTCAATTTGGTAAAATCAAATGAACTAAGGAGAGAAGTTTCCCATTCATTTCCTGTTTTAATTTTGTGTACTAGTTTATTTTCACTGAGAAACAATTCGTGATTTCAGAGATCCCAAGTTACGTCATTGTTTGGAGCAGAATCTCTTTGGGCAAAGGGGTATACCGGTTCTAAAGTCAGAATGGCTATATTTGATACTGGTATTCGTGCCAATCACCCACATTTTAGGAATATCAAGGTAATAAATATTAGGATTTGTTTAATTTAATGTGTCTTTTGTTTAATTATTAGATCAGAATAATTTTTCCACCCATAGAGATACATTTCACATGATAGATTTTGAATTTTGAGTAATTTTCAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGGCATGGGACATTTGTTGCCGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGGTTTGCTCCAGATACTGAAATATATGCTTTTCGTGTGTTTACAGATGCACAGGTAATGCACAATGATCATTACTCAATTGTAATATGTTTATTAGATCTTTTTACTTCAAATTAGTGTTTTATCAATTTACCTTAGTTTATCTTCAAACACAGTGGAAGGATTATGGAAGTTTTTTGTTTCTTATCCATCCAAAAGAAGGATTATGAAAAGTATGTATTCAGCCTATTGCAGGTCTCATACACTTCATGGTTCCTTGATGCATTCAACTACGCTATTGCTACCAATATGGATGTATTGAATTTAAGCATTGGTGGACCAGATTACTTAGATCTTCCATTTGTGGAGAAGGTAAGATTTCTTTTTTCTGCTGATTGCAATTGGTGGTCTGGCAAAATAAGTTATGAACTTGAAAAGAATATCACTTAATTTTATTTGGTTTGAGGTTGATCAATTGCTGTATATGTCTCGTTTGAACAAACATTTCCTACATGTATGTTTTCTATTAAATTATTTATACCTTTTTTCAGGTTAGATGTTGCCAGACATGTGTTACAAAGAGCATTAGATAAATGAAATGTGTGTCATCTTTGGGATACTGTCAATTATTTTTTCATGATATATAATACAGTTCTTGGACTACCTACAGTCTGGTAGTCTTATTAATCTTGTCTTAACATGATCATTGGTGAATATGTTGTTGAGGACTTCCTCGTGTGAAATAATTTTATACTTTATGAAGTTTCAACTTAAAATTCACTAGCTTGTTGAAGCTGTTTTTGGTTTTGTAAATTATGGCCACTCGTTTGTATCTCTAGTTTTAACTTTTGGGTTTCTGGTTGCAACCTATTGATAGGTGAGAGGTCTATAGTCAGAATCCATGCATCAGAAATGATTCTTTCTAATCAATTGAATGTCTATACATTAGGCTTCACATAATCATGCTAAGAGTAACTGCAAGCTGATAAGTGCAAAGAAATTATGATAGCATCAAAGTATATTTAACTGCTATTTGTCTACCAGATTTGGGAGATCACGGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTCTATGGGACTTTGAACAATCCTGCAGATCAGAGTGATGTAATTGGCGTTGGTGGTATTGACTATAATGATCATATTGCTTCATTTTCCTCCCGTGGCATGACTACTTGGGAGATGCCTCATGGGTAAATTACGCTTTACTTGGGATCTTTCAGTATTTTGTTCCTATCTTTCATAAGATATCTTGAGATATTAGGTAGAAAGTTTGTCTCCTTTCTGAGATTGGAGTTTGGCCTTGGATAAAGTATGTGTCGGATGCATTAAAATACAAATTTAAGCACTTACTGTTTTTAACTTGTGCAAGTCAGACCAAAGTCTTGTTAAAGATAAAATGGATTTATTTTAACATAATACTACTTTCCCTTATTTTCCTTCCTTTTTCTGTCTACCCATATCGGCACTCTCAAACATGTTATTTGGTACAGTTATGGTCGTGTGAAGCCAGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGGTGCAAAAGTTTATCAGGCACTAGTGTGGCGAGCCCTGTGGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGGAGAATATTTTAAATCCAGCTAGCATGAAACAAGCACTTGTTGAGGGAGCTGCAAAGCTTGCTGGTCCCAATGTGTACGAGCAAGGTGCTGGCAGGGTTGATCTGTAGGTTTCAGCTCTCATTGCTGTTGTTATTTATCTAGCTTGCCTCAAGAGTATTTTCTTATTCCTCTGAATTTTCCCAGGTTAGAATCGTACGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAATGTTCTTGACTATACCGATTGCCCTTATATGTGGCCCTTTTGTCGCCAACCACTTTATGCAGGTGCAATGCCTATTATCTTCAATGCTACCATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCATCCTTCGGATGAAGAAGGAAATCTTTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAAGGCAATGTGACTCTAACAGTATATAGTCCTCCATCTAGTGGAGAGAAAAACCGCCGGACTAGTACCTGTGTGCTACATCTGAAGTTAAAAGTGGTTCCGACACCTCCAAGGTCAAAACGTATTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCATACAAATTTTCATATCATGTTTAATATGTTGAGAGATGCTGGGTACTATGTTGAAACGCTGGGTTCTCCTCTTACATGCTTTGATTCTCTCCAATATGGCACACTTCTACTAGTGGATCTAGAAGATGAGTACTTCAAAGAGGAAATTGAAAAGTTGAGGGACGATGTTATGGTTACTGGATTGGGATTAGCTGTGTTTTCTGAATGGTATAATGTAGAAACAATGGTAAAGATGAGGTTCTTTGATGATAACACAAGGAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCATTAAATGACCTTTTAGCCCCATTTGGTATTGCTTTTGGGGATAAGATTTTGAACGGTGATTTTTCTATTGACGGTGAACAGAGTCGATATGCATCGGGAACAGATATTGTGAGGTTTCCACGAGGTGGTTATGTGCATAGTTTCCCTTTCATTGATAGCTCAGAGAGTGGGGCAGCGCAGAACATACTAACCTCTGGCATGACTAAGGTAACTGCCACAAGCTTCTCTATGAACTCTTTTTAAATAATTTTTGTGGTGACCACAAATTGTTGCAAATTCGACTAATGGTCCAGTTTCCTATTTTGGAAGCTTAAATAATTGTGGACAACTCTGTACTTTAGTTGAAGAAGATACTTATACATTTCATATCTTGCACCAGACGGAATTAGTAAATTGTTGGAGCAGCATGCCTATTATAACTTGCTGGTGCATAAATGTATTACTTGAGAAGATAGTTTCTTGACTCTGGTGAAAGGTGCACGAGACCACACAAGAATTTCCAATCTTCATTTTCCTTTATGCATTAATTGGAAATCTTATGAGTTGAGAATATTTAAATATCTAAATATCTGAAAGTTGTTGGGAAGAATGTATCTAAATGTTCGAATTATATTTATAGGCTTTTTTGGTAACATTCTTGACATTTAAGAGCCTTCCAAGGGAAGGTGTACGTTTTCATGAGGCTATTGTCATCTCTTTAATAGGATTAAACTAACTGTTTATTGACAAATGTAGGCAGACTTTCCAATTCTTGGTCTATTGGAGGAGGTGGGAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGATAGCAGCCATATGGTTACAAATTGCTATTGGCTTCTGAGAAAAATATTAGATTTTACCAGTGGAAACATTAGAGATCCGGTGCTCTTCACTAAGTCTTCAAAACTAGATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTATGTATTCTGTTGTTGTTGGAAAGGAGTTGATATGCAGGAGTGATTCTAGGTTTGATACATGGGGGACCAAGGGATACAATATACAGGTCAGAGGAAGAAATAGAAGGTTGCCCGGATTTCCCATAATTGATTTGGGAAGAGGCTTGAATTCTACATCAGAAACTTCTTCGATGAGACCTCCCAAGTTGACTTCAGAAAACCGAAGTGATTCATATGGAAACCGATACTTAAACCTGTTCTACGGGGACGAGGTGAGAGTTCTTTCCCAATGAGTTGAGACTTAAATTACAATATTATTTCATCAATATGATTCTTGTAATTTCCTGTTCATTGTTCTTGCTTACAGCCTGACATGCCTTTGATTGTACCTAATCATTGGCTTGTTCCTGCCGTAGTTGCAGTGACAGGTGAGTGATATATTCTCTTGTTGCTGCTGAATTTGATTTCCTCTTTCAAAGTTTATACTTGGTTTCGATGCTTTTTAGGGCTTTTGCTCCTTTTGAGTTTTTGGCGGATCAGACAGAGGCGGCGACGGAGACGAAGAGGATCTGGATCTGCACGTTTTCCCAATCTATAG

mRNA sequence

ATGATCAGTTCTTCGGTTTCTTCTTCATATGTCTCCTTCGTCCTCATCTTCGTTCCCATCTTTATCTCTATCTCTCTCTTCCAATTCAAACCCTCTTCCGATCCTATTGTATATCAAACCCTAACCCGAAACTACTCGTCGGTCGATCCATTTACCTCCAAGAACCTCAGTGACGGGAACCAAGTCTCGCGGAAGAACTACATTGTTCGCTTCGTCCAATACAGGGAGGCGAAGGAGCACCGGTCCTATCTGGAATCGCGTATTAGATCGGGTCTCTGGGAATGGATCGAGAGGAAGAACCCTGCGTCTAAGTATCCAACCGAGTTTGGCTTGGTTTCTATTGAGGACTCGGCGAGAGGGGAGTTGATTAAGGATATTGAGGAATTAGAGCTCGTCAAGGATGTGAATGTGGATGCAAGCTACGTGAGGGACGTGCTAACGGAGAATGGGGGTAGAGTCGGGGCTTTTGTAGACGGGAAGAAGCGTCCGGGGAAGATTTTCACCTCCATGTCGTTTAAGGAAGGAGGCGGGGAACGTTATACTGCAATTAGCAATGCCTCTAATAGATGGGGACGGCATCTGTCGATGCAGAGATCCCAAGTTACGTCATTGTTTGGAGCAGAATCTCTTTGGGCAAAGGGGTATACCGGTTCTAAAGTCAGAATGGCTATATTTGATACTGGTATTCGTGCCAATCACCCACATTTTAGGAATATCAAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGGCATGGGACATTTGTTGCCGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGGTTTGCTCCAGATACTGAAATATATGCTTTTCGTGTGTTTACAGATGCACAGGTCTCATACACTTCATGGTTCCTTGATGCATTCAACTACGCTATTGCTACCAATATGGATGTATTGAATTTAAGCATTGGTGGACCAGATTACTTAGATCTTCCATTTGTGGAGAAGATTTGGGAGATCACGGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTCTATGGGACTTTGAACAATCCTGCAGATCAGAGTGATGTAATTGGCGTTGGTGGTATTGACTATAATGATCATATTGCTTCATTTTCCTCCCGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCAGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGGTGCAAAAGTTTATCAGGCACTAGTGTGGCGAGCCCTGTGGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGGAGAATATTTTAAATCCAGCTAGCATGAAACAAGCACTTGTTGAGGGAGCTGCAAAGCTTGCTGGTCCCAATGTGTACGAGCAAGGTGCTGGCAGGGTTGATCTGTTAGAATCGTACGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAATGTTCTTGACTATACCGATTGCCCTTATATGTGGCCCTTTTGTCGCCAACCACTTTATGCAGGTGCAATGCCTATTATCTTCAATGCTACCATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCATCCTTCGGATGAAGAAGGAAATCTTTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAAGGCAATGTGACTCTAACAGTATATAGTCCTCCATCTAGTGGAGAGAAAAACCGCCGGACTAGTACCTGTGTGCTACATCTGAAGTTAAAAGTGGTTCCGACACCTCCAAGGTCAAAACGTATTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCATACAAATTTTCATATCATGTTTAATATGTTGAGAGATGCTGGGTACTATGTTGAAACGCTGGGTTCTCCTCTTACATGCTTTGATTCTCTCCAATATGGCACACTTCTACTAGTGGATCTAGAAGATGAGTACTTCAAAGAGGAAATTGAAAAGTTGAGGGACGATGTTATGGTTACTGGATTGGGATTAGCTGTGTTTTCTGAATGGTATAATGTAGAAACAATGGTAAAGATGAGGTTCTTTGATGATAACACAAGGAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCATTAAATGACCTTTTAGCCCCATTTGGTATTGCTTTTGGGGATAAGATTTTGAACGGTGATTTTTCTATTGACGGTGAACAGAGTCGATATGCATCGGGAACAGATATTGTGAGGTTTCCACGAGGTGGTTATGTGCATAGTTTCCCTTTCATTGATAGCTCAGAGAGTGGGGCAGCGCAGAACATACTAACCTCTGGCATGACTAAGGCAGACTTTCCAATTCTTGGTCTATTGGAGGAGGTGGGAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGATAGCAGCCATATGGTTACAAATTGCTATTGGCTTCTGAGAAAAATATTAGATTTTACCAGTGGAAACATTAGAGATCCGGTGCTCTTCACTAAGTCTTCAAAACTAGATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTATGTATTCTGTTGTTGTTGGAAAGGAGTTGATATGCAGGAGTGATTCTAGGTTTGATACATGGGGGACCAAGGGATACAATATACAGGTCAGAGGAAGAAATAGAAGGTTGCCCGGATTTCCCATAATTGATTTGGGAAGAGGCTTGAATTCTACATCAGAAACTTCTTCGATGAGACCTCCCAAGTTGACTTCAGAAAACCGAAGTGATTCATATGGAAACCGATACTTAAACCTGTTCTACGGGGACGAGCCTGACATGCCTTTGATTGTACCTAATCATTGGCTTGTTCCTGCCGTAGTTGCAGTGACAGGGCTTTTGCTCCTTTTGAGTTTTTGGCGGATCAGACAGAGGCGGCGACGGAGACGAAGAGGATCTGGATCTGCACGTTTTCCCAATCTATAG

Coding sequence (CDS)

ATGATCAGTTCTTCGGTTTCTTCTTCATATGTCTCCTTCGTCCTCATCTTCGTTCCCATCTTTATCTCTATCTCTCTCTTCCAATTCAAACCCTCTTCCGATCCTATTGTATATCAAACCCTAACCCGAAACTACTCGTCGGTCGATCCATTTACCTCCAAGAACCTCAGTGACGGGAACCAAGTCTCGCGGAAGAACTACATTGTTCGCTTCGTCCAATACAGGGAGGCGAAGGAGCACCGGTCCTATCTGGAATCGCGTATTAGATCGGGTCTCTGGGAATGGATCGAGAGGAAGAACCCTGCGTCTAAGTATCCAACCGAGTTTGGCTTGGTTTCTATTGAGGACTCGGCGAGAGGGGAGTTGATTAAGGATATTGAGGAATTAGAGCTCGTCAAGGATGTGAATGTGGATGCAAGCTACGTGAGGGACGTGCTAACGGAGAATGGGGGTAGAGTCGGGGCTTTTGTAGACGGGAAGAAGCGTCCGGGGAAGATTTTCACCTCCATGTCGTTTAAGGAAGGAGGCGGGGAACGTTATACTGCAATTAGCAATGCCTCTAATAGATGGGGACGGCATCTGTCGATGCAGAGATCCCAAGTTACGTCATTGTTTGGAGCAGAATCTCTTTGGGCAAAGGGGTATACCGGTTCTAAAGTCAGAATGGCTATATTTGATACTGGTATTCGTGCCAATCACCCACATTTTAGGAATATCAAGGAGCGAACAAATTGGACCAATGAAGATACTTTAAATGATAATCTTGGGCATGGGACATTTGTTGCCGGTGTCATTGCAGGTGGAGATGAAGAGTGTCTAGGGTTTGCTCCAGATACTGAAATATATGCTTTTCGTGTGTTTACAGATGCACAGGTCTCATACACTTCATGGTTCCTTGATGCATTCAACTACGCTATTGCTACCAATATGGATGTATTGAATTTAAGCATTGGTGGACCAGATTACTTAGATCTTCCATTTGTGGAGAAGATTTGGGAGATCACGGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTCTATGGGACTTTGAACAATCCTGCAGATCAGAGTGATGTAATTGGCGTTGGTGGTATTGACTATAATGATCATATTGCTTCATTTTCCTCCCGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCAGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGGTGCAAAAGTTTATCAGGCACTAGTGTGGCGAGCCCTGTGGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGGAGAATATTTTAAATCCAGCTAGCATGAAACAAGCACTTGTTGAGGGAGCTGCAAAGCTTGCTGGTCCCAATGTGTACGAGCAAGGTGCTGGCAGGGTTGATCTGTTAGAATCGTACGAAATACTTAAGAGCTACCAACCCCGGGCAAGCATCTTCCCTAATGTTCTTGACTATACCGATTGCCCTTATATGTGGCCCTTTTGTCGCCAACCACTTTATGCAGGTGCAATGCCTATTATCTTCAATGCTACCATTTTGAATGGGATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCATCCTTCGGATGAAGAAGGAAATCTTTTAAGCATTCACTTCACTTATTCTCAAGTCATCTGGCCTTGGACTGGTTATATTGCACTTCACATGCAAATTAAGGAAGAAGGTGCACAGTTTTCTGGAGAAATTGAAGGCAATGTGACTCTAACAGTATATAGTCCTCCATCTAGTGGAGAGAAAAACCGCCGGACTAGTACCTGTGTGCTACATCTGAAGTTAAAAGTGGTTCCGACACCTCCAAGGTCAAAACGTATTTTATGGGATCAATTTCATAGTATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCATACAAATTTTCATATCATGTTTAATATGTTGAGAGATGCTGGGTACTATGTTGAAACGCTGGGTTCTCCTCTTACATGCTTTGATTCTCTCCAATATGGCACACTTCTACTAGTGGATCTAGAAGATGAGTACTTCAAAGAGGAAATTGAAAAGTTGAGGGACGATGTTATGGTTACTGGATTGGGATTAGCTGTGTTTTCTGAATGGTATAATGTAGAAACAATGGTAAAGATGAGGTTCTTTGATGATAACACAAGGAGCTGGTGGACTCCAGTTACTGGAGGTGCAAATATTCCTGCATTAAATGACCTTTTAGCCCCATTTGGTATTGCTTTTGGGGATAAGATTTTGAACGGTGATTTTTCTATTGACGGTGAACAGAGTCGATATGCATCGGGAACAGATATTGTGAGGTTTCCACGAGGTGGTTATGTGCATAGTTTCCCTTTCATTGATAGCTCAGAGAGTGGGGCAGCGCAGAACATACTAACCTCTGGCATGACTAAGGCAGACTTTCCAATTCTTGGTCTATTGGAGGAGGTGGGAGAGGGCCGCATTGCAGTCTATGGAGATTCAAATTGTCTGGATAGCAGCCATATGGTTACAAATTGCTATTGGCTTCTGAGAAAAATATTAGATTTTACCAGTGGAAACATTAGAGATCCGGTGCTCTTCACTAAGTCTTCAAAACTAGATTCACCTCTGTATCTAGAAGATAACAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTATGTATTCTGTTGTTGTTGGAAAGGAGTTGATATGCAGGAGTGATTCTAGGTTTGATACATGGGGGACCAAGGGATACAATATACAGGTCAGAGGAAGAAATAGAAGGTTGCCCGGATTTCCCATAATTGATTTGGGAAGAGGCTTGAATTCTACATCAGAAACTTCTTCGATGAGACCTCCCAAGTTGACTTCAGAAAACCGAAGTGATTCATATGGAAACCGATACTTAAACCTGTTCTACGGGGACGAGCCTGACATGCCTTTGATTGTACCTAATCATTGGCTTGTTCCTGCCGTAGTTGCAGTGACAGGGCTTTTGCTCCTTTTGAGTTTTTGGCGGATCAGACAGAGGCGGCGACGGAGACGAAGAGGATCTGGATCTGCACGTTTTCCCAATCTATAG

Protein sequence

MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGNQVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARGELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDLLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLRDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFPILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDSPLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFPIIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVVAVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL
Homology
BLAST of Moc06g07730 vs. NCBI nr
Match: XP_022144866.1 (subtilisin-like protease SBT6.1 isoform X1 [Momordica charantia])

HSP 1 Score: 2149.8 bits (5569), Expect = 0.0e+00
Identity = 1053/1053 (100.00%), Postives = 1053/1053 (100.00%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN
Sbjct: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG
Sbjct: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY
Sbjct: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS
Sbjct: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV
Sbjct: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL 1054
            AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL
Sbjct: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL 1053

BLAST of Moc06g07730 vs. NCBI nr
Match: XP_038879333.1 (subtilisin-like protease SBT6.1 [Benincasa hispida])

HSP 1 Score: 1984.9 bits (5141), Expect = 0.0e+00
Identity = 963/1048 (91.89%), Postives = 1010/1048 (96.37%), Query Frame = 0

Query: 6    VSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGNQVSRK 65
            ++SS +SF++IFVPIFISISLFQFKPSSD  V+QTLTRNYS VDP  SKNL+DGN V RK
Sbjct: 2    IASSSISFIVIFVPIFISISLFQFKPSSDHTVFQTLTRNYSPVDPSPSKNLTDGN-VLRK 61

Query: 66   NYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARGELIKD 125
            +YIVRF+QYREAKEHR YLESRIRSG WEWIER+NPASKYPT+FGLVSIEDS RGELIK+
Sbjct: 62   SYIVRFLQYREAKEHRFYLESRIRSGGWEWIERRNPASKYPTDFGLVSIEDSVRGELIKE 121

Query: 126  IEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAISN 185
            IEELE VKDVNVDA++VR +L E+GGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAISN
Sbjct: 122  IEELEFVKDVNVDATHVRGLLMEDGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAISN 181

Query: 186  ASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIKERTNW 245
            ASN WGRHLSM+RSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIR+NHPHFRNIKERTNW
Sbjct: 182  ASNGWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRSNHPHFRNIKERTNW 241

Query: 246  TNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYA 305
            TNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYA
Sbjct: 242  TNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYA 301

Query: 306  IATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVI 365
            IATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVI
Sbjct: 302  IATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVI 361

Query: 366  GVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVA 425
            GVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVA
Sbjct: 362  GVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVA 421

Query: 426  SPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDLLESYE 485
            SPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDLLESYE
Sbjct: 422  SPVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYE 481

Query: 486  ILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPT 545
            ILKSYQPRASIFP+VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPT
Sbjct: 482  ILKSYQPRASIFPDVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPT 541

Query: 546  WHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSSG 605
            WHP DEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPS G
Sbjct: 542  WHPLDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRG 601

Query: 606  EKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDH 665
            EKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDH
Sbjct: 602  EKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDH 661

Query: 666  LHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLRDDVMV 725
            LHTNFHIMFNML DAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLRDDVM+
Sbjct: 662  LHTNFHIMFNMLSDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMI 721

Query: 726  TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILN 785
            TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILN
Sbjct: 722  TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILN 781

Query: 786  GDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFPILGLL 845
            GDFSIDGEQSR+ASGTDIVRFP+GGYVHSFPF+DSSESGAAQ+ILTS M+KADFPILGLL
Sbjct: 782  GDFSIDGEQSRFASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFPILGLL 841

Query: 846  EEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDSPLYLE 905
            E + EGR+AVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK DSPLYLE
Sbjct: 842  EAI-EGRVAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRDSPLYLE 901

Query: 906  DNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFPIIDLG 965
            DNKLPSRRSDVNFS+YS V GKEL+CRSDSRF+ WGTKGY+IQVRGRNRRLPGFP+IDLG
Sbjct: 902  DNKLPSRRSDVNFSLYSAVAGKELLCRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLG 961

Query: 966  RGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVVAVTGL 1025
            RGLNST E+SSM PPKLT ++R D+YGNRYL+LFY DEPDMPLIVPN WLVPAVVA+TGL
Sbjct: 962  RGLNSTLESSSMGPPKLTLKDRRDTYGNRYLSLFYRDEPDMPLIVPNQWLVPAVVALTGL 1021

Query: 1026 LLLLSFWRIRQRRRRRRRGSGSARFPNL 1054
            LLLLSFWRIRQ+RRRRRRGSGSARF N+
Sbjct: 1022 LLLLSFWRIRQKRRRRRRGSGSARFSNI 1047

BLAST of Moc06g07730 vs. NCBI nr
Match: XP_022987592.1 (subtilisin-like protease SBT6.1 [Cucurbita maxima])

HSP 1 Score: 1974.1 bits (5113), Expect = 0.0e+00
Identity = 964/1054 (91.46%), Postives = 1005/1054 (95.35%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MI  S+SSS +SF +IFVPIFISIS+FQF PSSD  V+QTLTRNYS  DPFTSKN++DGN
Sbjct: 1    MIGFSISSSSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            +VS+KNYIVRFVQYREAKEHR YLESRIRSG W+WIER+NPASKYPT+FGLVSIEDS   
Sbjct: 61   RVSQKNYIVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVR 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            EL+++IEE ELVKDVNVDAS+VR +L E GGR+GAFVDGKKRPGKIFTSMSF EGGGERY
Sbjct: 121  ELVEEIEEFELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEGGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHLSMQRSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLSMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTW+PSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWNPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPS GEKNRR STCVL LKLKVV TPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSRGEKNRRISTCVLQLKLKVVSTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVM+TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPR GYVHSFPFIDSSESGAAQ+ILTS ++KADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRDGYVHSFPFIDSSESGAAQSILTSSLSKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLL E GEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK +S
Sbjct: 841  ILGLL-EAGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSDVNFS+YS V GKELIC SDSRF+ WGTKGY++Q RGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            +IDLGR LNST E+S M PPKLT ++RSD YGNRYL+LFY DEPDMPLIVPNHWLVPAVV
Sbjct: 961  LIDLGRDLNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSA-RFPNL 1054
            AV GLLLLLSFWRIRQ+RRRRRRGSGSA RF NL
Sbjct: 1021 AVAGLLLLLSFWRIRQKRRRRRRGSGSAPRFSNL 1053

BLAST of Moc06g07730 vs. NCBI nr
Match: KAG6589917.1 (Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023586.1 Subtilisin-like protease SBT6.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1973.7 bits (5112), Expect = 0.0e+00
Identity = 964/1054 (91.46%), Postives = 1005/1054 (95.35%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MI  S+SSS +SF +IFVPIFISIS+FQF PSSD  V+QTLTRNYS  DPFTSKN++DGN
Sbjct: 1    MIGVSISSSSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            +VS+KNYIVRFVQYREAKEHR YLESRIRSG W+WIER+NPASKYPT+FGLVSIEDS   
Sbjct: 61   RVSQKNYIVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVR 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            EL+++IEELELVKDVNVDAS+VR +L E GGR+GAFVDGKKRPGKIFTSMSF E GGERY
Sbjct: 121  ELVEEIEELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHL MQRSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTW+PSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWNPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPS GEKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSRGEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVM+TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQ+ILTS ++KADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLL E  EGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK +S
Sbjct: 841  ILGLL-EAAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSDVNFS+YS V GKELIC SDSRF+ WGTKGY++Q RGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            +IDLGR LNST E+S M PPKLT ++RSD YGNRYL+LFY DEPDMPLIVPNHWLVPAVV
Sbjct: 961  LIDLGRDLNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGS-ARFPNL 1054
            AV GLLLLLSFWRIRQ+RRRRRRGSGS ARF NL
Sbjct: 1021 AVAGLLLLLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Moc06g07730 vs. NCBI nr
Match: XP_022960716.1 (subtilisin-like protease SBT6.1 [Cucurbita moschata])

HSP 1 Score: 1971.8 bits (5107), Expect = 0.0e+00
Identity = 963/1054 (91.37%), Postives = 1004/1054 (95.26%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MI  S+SSS +SF +IFVPIFISIS+FQF PSSD  V+QTLTRNYS  DPFTSKN++DGN
Sbjct: 1    MIGYSISSSSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            +VS+KNYIVRFVQYREAKEHR YLESRIRSG W+WIER+NPASKYPT+FGLVSIEDS   
Sbjct: 61   RVSQKNYIVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVM 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            EL+++IEELELVKDVNVDAS+VR +L E GGR+GAFVDGKKRPGKIFTSMSF E GGERY
Sbjct: 121  ELVEEIEELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHL MQRSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTW+PSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWNPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPS GEKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSRGEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVM+TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQ+ILTS ++KADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLL E  EGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK +S
Sbjct: 841  ILGLL-EAAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSD NFS+YS V GKELIC SDSRF+ WGTKGY++Q RGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDANFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            +IDLGR LNST E+S M PPKLT ++RSD YGNRYL+LFY DEPDMPLIVPNHWLVPAVV
Sbjct: 961  LIDLGRDLNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGS-ARFPNL 1054
            AV GLLLLLSFWRIRQ+RRRRRRGSGS ARF NL
Sbjct: 1021 AVAGLLLLLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Moc06g07730 vs. ExPASy Swiss-Prot
Match: Q0WUG6 (Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 SV=1)

HSP 1 Score: 1595.1 bits (4129), Expect = 0.0e+00
Identity = 779/1054 (73.91%), Postives = 892/1054 (84.63%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            ++  + S  Y S +++   +F+S+SLF  +PS       T      +++P     L   N
Sbjct: 3    VLGEASSYPYRSCIIV---VFLSVSLFWLRPS-------TYHPQQQNLNPENVTRLESEN 62

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            + ++ NYI+RF QY+ AK+HR YLES++RSG W WIER NPA+KYPT+FG++ IE+S + 
Sbjct: 63   E-TKTNYIIRFKQYKPAKDHRIYLESKVRSGGWGWIERINPATKYPTDFGVLWIEESGKE 122

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
             ++ +IE LE+VKDVNV+  Y R +L       G+F DGKKRPGKIFTSMSF+EG     
Sbjct: 123  AVVGEIERLEMVKDVNVEFKYQRVLLG------GSFPDGKKRPGKIFTSMSFEEGTESSP 182

Query: 181  TA-ISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNI 240
             A  SN +  W RHL  Q++QVTS+FGA+ LW KGYTG+KV+MAIFDTGIRA+HPHFR I
Sbjct: 183  MADTSNTTLNWSRHLLAQKTQVTSMFGADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKI 242

Query: 241  KERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFL 300
            KERTNWTNEDTLNDNLGHGTFVAGVIAG + ECLGFA DTEIYAFRVFTDAQVSYTSWFL
Sbjct: 243  KERTNWTNEDTLNDNLGHGTFVAGVIAGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFL 302

Query: 301  DAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPA 360
            DAFNYAIAT+MDVLNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPA
Sbjct: 303  DAFNYAIATDMDVLNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPA 362

Query: 361  DQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSL 420
            DQSDVIGVGGID +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSL
Sbjct: 363  DQSDVIGVGGIDNDDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSL 422

Query: 421  SGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVD 480
            SGTSVASPVVAG+VCLLVSVIPE++R+++LNPASMKQALVEGAAKL+GPN+YEQGAGRVD
Sbjct: 423  SGTSVASPVVAGIVCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVD 482

Query: 481  LLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGY 540
            LLESYEILKSY PRASIFP++LDY DCPY WPFCRQPLYAGAMPIIFN TILNGMGVIGY
Sbjct: 483  LLESYEILKSYHPRASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGY 542

Query: 541  VEGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVY 600
            +E  PTWHP++EEGNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VY
Sbjct: 543  IESPPTWHPANEEGNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVY 602

Query: 601  SPPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDIL 660
            SPP+SGE   R STC L LKLKV+PTPPR+KRILWDQFHSIKYPPGYIPRDSLDVRNDIL
Sbjct: 603  SPPASGESGPRRSTCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDIL 662

Query: 661  DWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKL 720
            DWHGDHLHTNFHIM+NMLRDAGYY+ETLGSPLTCFD+ QYGTLL+VDLED+YF EEIEKL
Sbjct: 663  DWHGDHLHTNFHIMYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKL 722

Query: 721  RDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAF 780
            RDDV+ TGLGL VF+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAF
Sbjct: 723  RDDVINTGLGLVVFAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAF 782

Query: 781  GDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADF 840
            GDKILNGDFSIDGEQSRYASGT+IVRFP GG++H+FP +DSSESGA QN+L +  +K D 
Sbjct: 783  GDKILNGDFSIDGEQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDP 842

Query: 841  PILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLD 900
             +LGLL E+GEGR+ VYGDSNCLDSSHMVTNCYWLL+K+LDF+S NI+DPVLF+K +K  
Sbjct: 843  AVLGLL-EIGEGRVGVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRY 902

Query: 901  SPLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGF 960
            SP+ +++ +LPSRR+DVNFS YS V+GKELIC SDSRF+ WGTKGYN+ VRGRNRRLPG+
Sbjct: 903  SPVIIDEKQLPSRRTDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGY 962

Query: 961  PIIDLGRGLNSTSETSSMRPPKLTSENR----SDSYGNRYLNLFYGDEPDMPLIVPNHWL 1020
              IDLGRGLN T E  S RP +  S       S S       LF  DE DMP +VP  W+
Sbjct: 963  HGIDLGRGLNFTVE--SKRPTRWRSAKEGGELSSSRSKSLGGLFNRDEIDMPFLVPTRWI 1022

Query: 1021 VPAVVAVTGLLLLLSFWRIRQRRRRRRRGSGSAR 1050
            V A V  +G+L+LLS WRIRQ+R RRRR SGS R
Sbjct: 1023 VLAGVVASGVLVLLSIWRIRQKRGRRRRASGSNR 1036

BLAST of Moc06g07730 vs. ExPASy Swiss-Prot
Match: Q14703 (Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=MBTPS1 PE=1 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 3.9e-257
Identity = 445/885 (50.28%), Postives = 587/885 (66.33%), Query Frame = 0

Query: 67  YIVRFVQYREAKEHRSYLESRIRSG---LWEWIERKNPASKYPTEFGLVSIEDSARGELI 126
           YIV F  Y  AK   S++ S ++S     W  I R NP+S YP++F ++ I++  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 127 KDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAI 186
             +E+   +K V       R +         A  D      +   S  ++     R  ++
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKY-------AESDPTVPCNETRWSQKWQSSRPLRRASL 175

Query: 187 SNASNRW---GRHLSMQ-----RSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPH 246
           S  S  W   GRH S +       QV     A+ LW  GYTG+ VR+A+FDTG+   HPH
Sbjct: 176 SLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPH 235

Query: 247 FRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYT 306
           F+N+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSYT
Sbjct: 236 FKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSYT 295

Query: 307 SWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTL 366
           SWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTL
Sbjct: 296 SWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTL 355

Query: 367 NNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTG 426
           NNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGR+KPD+V YG  + GS +  G
Sbjct: 356 NNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRMKPDIVTYGAGVRGSGVKGG 415

Query: 427 CKSLSGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGA 486
           C++LSGTSVASPVVAG V LLVS +   Q+  ++NPASMKQAL+  A +L G N++EQG 
Sbjct: 416 CRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASMKQALIASARRLPGVNMFEQGH 475

Query: 487 GRVDLLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMG 546
           G++DLL +Y+IL SY+P+AS+ P+ +D T+CPYMWP+C QP+Y G MP + N TILNGMG
Sbjct: 476 GKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTVVNVTILNGMG 535

Query: 547 VIGYVEGQPTWHP-SDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNV 606
           V G +  +P W P   + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+V
Sbjct: 536 VTGRIVDKPDWQPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHV 595

Query: 607 TLTVYSPPSSGEKN--RRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSL 666
            +TV SP  +  KN   +TST  L +K+K++PTPPRSKR+LWDQ+H+++YPPGY PRD+L
Sbjct: 596 MITVASPAETESKNGAEQTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNL 655

Query: 667 DVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYF 726
            ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFD+ QYGTLL+VD E+EYF
Sbjct: 656 RMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPFTCFDASQYGTLLMVDSEEEYF 715

Query: 727 KEEIEKLRDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLL 786
            EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL
Sbjct: 716 PEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELL 775

Query: 787 APFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFID------SSESGAA 846
           + + + F D +  G+F++      YASG  I +FP  G V +  F D        E+   
Sbjct: 776 SVWNMGFSDGLYEGEFTLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAVV 835

Query: 847 QNILTSGMTKADFPILGL--LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSG 906
           +N+          PILGL  +   G GRI +YGDSNCLD SH   +C+WLL  +L +TS 
Sbjct: 836 ENV----------PILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSY 895

Query: 907 NIRDPVLFTKSSKLDSPLYLEDNKLPSRRSDVNFSMYSVVVGKEL 930
            +  P L + S     P     +  P R    +   YS V+   L
Sbjct: 896 GVTPPSL-SHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEAHL 916

BLAST of Moc06g07730 vs. ExPASy Swiss-Prot
Match: Q9Z2A8 (Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=10029 GN=MBTPS1 PE=1 SV=2)

HSP 1 Score: 885.9 bits (2288), Expect = 4.3e-256
Identity = 436/857 (50.88%), Postives = 577/857 (67.33%), Query Frame = 0

Query: 67  YIVRFVQYREAKEHRSYLESRIRSG---LWEWIERKNPASKYPTEFGLVSIEDSARGELI 126
           YIV F  Y  AK   S++ S ++S     W  I R NP+S YP++F ++ I++  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 127 KDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAI 186
             +E+   +K V       R +       +    + +        S  ++     R  ++
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETR-------WSQKWQSSRPLRRASL 175

Query: 187 SNASNRW---GRHLSMQ-----RSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPH 246
           S  S  W   GRH S +       QV     A+ LW  GYTG+ VR+A+FDTG+   HPH
Sbjct: 176 SLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPH 235

Query: 247 FRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYT 306
           F+N+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSYT
Sbjct: 236 FKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSYT 295

Query: 307 SWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTL 366
           SWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTL
Sbjct: 296 SWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTL 355

Query: 367 NNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTG 426
           NNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  G
Sbjct: 356 NNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGG 415

Query: 427 CKSLSGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGA 486
           C++LSGTSVASPVVAG V LLVS +   Q+  ++NPAS+KQAL+  A +L G N++EQG 
Sbjct: 416 CRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGH 475

Query: 487 GRVDLLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMG 546
           G++DLL +Y+IL SY+P+AS+ P+ +D T+CPYMWP+C QP+Y G MP I N TILNGMG
Sbjct: 476 GKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMG 535

Query: 547 VIGYVEGQPTWHP-SDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNV 606
           V G +  +P W P   + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++
Sbjct: 536 VTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHI 595

Query: 607 TLTVYSPPSSGEKN--RRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSL 666
            +TV SP  +  KN    TST  L +K+K++PTPPRSKR+LWDQ+H+++YPPGY PRD+L
Sbjct: 596 MITVASPAETEAKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNL 655

Query: 667 DVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYF 726
            ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFD+ QYGTLL+VD E+EYF
Sbjct: 656 RMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYF 715

Query: 727 KEEIEKLRDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLL 786
            EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL
Sbjct: 716 PEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELL 775

Query: 787 APFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFID------SSESGAA 846
           + + + F D +  G+F++      YASG  I +FP  G V +  F D        E+   
Sbjct: 776 SVWNMGFSDGLYEGEFALANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAVV 835

Query: 847 QNILTSGMTKADFPILGL--LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSG 902
           +N+          PILGL  +   G GRI +YGDSNCLD SH   +C+WLL  +L +TS 
Sbjct: 836 ENV----------PILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSY 889

BLAST of Moc06g07730 vs. ExPASy Swiss-Prot
Match: Q9WTZ3 (Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=10116 GN=Mbtps1 PE=1 SV=2)

HSP 1 Score: 884.4 bits (2284), Expect = 1.3e-255
Identity = 435/851 (51.12%), Postives = 574/851 (67.45%), Query Frame = 0

Query: 67  YIVRFVQYREAKEHRSYLESRIRSG---LWEWIERKNPASKYPTEFGLVSIEDSARGELI 126
           YIV F  Y  AK   S++ S ++S     W  I R NP+S YP++F ++ I++  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 127 KDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAI 186
             +E+   +K V       R +       +    + +        S  ++     +  ++
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETR-------WSQKWQSSRPLKRASL 175

Query: 187 SNASNRW---GRHLSMQ-----RSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPH 246
           S  S  W   GRH S +       QV     A+ LW  GYTG+ VR+A+FDTG+   HPH
Sbjct: 176 SLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPH 235

Query: 247 FRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYT 306
           F+N+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSYT
Sbjct: 236 FKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSYT 295

Query: 307 SWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTL 366
           SWFLDAFNYAI   +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTL
Sbjct: 296 SWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTL 355

Query: 367 NNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTG 426
           NNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  G
Sbjct: 356 NNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGG 415

Query: 427 CKSLSGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGA 486
           C++LSGTSVASPVVAG V LLVS +   Q+  ++NPAS+KQAL+  A +L G N++EQG 
Sbjct: 416 CRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGH 475

Query: 487 GRVDLLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMG 546
           G++DLL +Y+IL SY+P+AS+ P+ +D T+CPYMWP+C QP+Y G MP I N TILNGMG
Sbjct: 476 GKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMG 535

Query: 547 VIGYVEGQPTWHP-SDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNV 606
           V G +  +P W P   + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++
Sbjct: 536 VTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHI 595

Query: 607 TLTVYSPPSSGEKN--RRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSL 666
            +TV SP  +  KN    TST  L +K+K++PTPPRSKR+LWDQ+H+++YPPGY PRD+L
Sbjct: 596 MITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNL 655

Query: 667 DVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYF 726
            ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFD+ QYGTLL+VD E+EYF
Sbjct: 656 RMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYF 715

Query: 727 KEEIEKLRDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLL 786
            EEI KLR DV   GL L VFS+WYN   M K++F+D+NTR WW P TGGAN+PALN+LL
Sbjct: 716 PEEIAKLRRDV-DNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELL 775

Query: 787 APFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTS 846
           + + + F D +  G+F++      YASG  I RFP  G V +  F D       Q     
Sbjct: 776 SVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVV 835

Query: 847 GMTKADFPILGL--LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPV 902
                + PILGL  +   G GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P 
Sbjct: 836 D----NVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPS 889

BLAST of Moc06g07730 vs. ExPASy Swiss-Prot
Match: Q9WTZ2 (Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=Mbtps1 PE=1 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 8.2e-255
Identity = 435/857 (50.76%), Postives = 575/857 (67.09%), Query Frame = 0

Query: 67  YIVRFVQYREAKEHRSYLESRIRSG---LWEWIERKNPASKYPTEFGLVSIEDSARGELI 126
           YIV F  Y  AK   S++ S ++S     W  I R NP+S YP++F ++ I++  +  L+
Sbjct: 56  YIVAFNGYFTAKARNSFISSALKSSEVENWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLL 115

Query: 127 KDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAI 186
             +E+   +K V       R +       +    + +        S  ++     +  ++
Sbjct: 116 -TLEDHPNIKRVTPQRKVFRSLKFAESNPIVPCNETR-------WSQKWQSSRPLKRASL 175

Query: 187 SNASNRW---GRHLSMQ-----RSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPH 246
           S  S  W   GRH S +       QV     A+ LW  GYTG+ VR+A+FDTG+   HPH
Sbjct: 176 SLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPH 235

Query: 247 FRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYT 306
           F+N+KERTNWTNE TL+D LGHGTFVAGVIA    EC GFAPD E++ FRVFT+ QVSYT
Sbjct: 236 FKNVKERTNWTNERTLDDGLGHGTFVAGVIA-SMRECQGFAPDAELHIFRVFTNNQVSYT 295

Query: 307 SWFLDAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTL 366
           SWFLDAFNYAI   MDVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTL
Sbjct: 296 SWFLDAFNYAILKKMDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTL 355

Query: 367 NNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTG 426
           NNPADQ DVIGVGGID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  G
Sbjct: 356 NNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGG 415

Query: 427 CKSLSGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGA 486
           C++LSGTSVASPVVAG V LLVS +   Q+  ++NPAS+KQAL+  A +L G N++EQG 
Sbjct: 416 CRALSGTSVASPVVAGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGH 475

Query: 487 GRVDLLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMG 546
           G++DLL +Y+IL SY+P+AS+ P+ +D T+CPYMWP+C QP+Y G MP I N TILNGMG
Sbjct: 476 GKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMG 535

Query: 547 VIGYVEGQPTWHP-SDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNV 606
           V G +  +P W P   + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++
Sbjct: 536 VTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHI 595

Query: 607 TLTVYSPPSSGEKN--RRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSL 666
            +TV SP  +   +    TST  L +K+K++PTPPRSKR+LWDQ+H+++YPPGY PRD+L
Sbjct: 596 MITVASPAETELHSGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNL 655

Query: 667 DVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYF 726
            ++ND LDW+GDH+HTNF  M+  LR  GY+VE LG+P TCFD+ QYGTLLLVD E+EYF
Sbjct: 656 RMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLLVDSEEEYF 715

Query: 727 KEEIEKLRDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLL 786
            EEI KLR DV   GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL
Sbjct: 716 PEEIAKLRRDV-DNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELL 775

Query: 787 APFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFID------SSESGAA 846
           + + + F D +  G+F +      YASG  I +FP  G V +  F D        E+   
Sbjct: 776 SVWNMGFSDGLYEGEFVLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAVV 835

Query: 847 QNILTSGMTKADFPILGL--LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSG 902
           +N+          PILGL  +   G GRI +YGDSNCLD SH   +C+WLL  +L +TS 
Sbjct: 836 ENV----------PILGLYQIPSEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSY 889

BLAST of Moc06g07730 vs. ExPASy TrEMBL
Match: A0A6J1CUP3 (subtilisin-like protease SBT6.1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014445 PE=3 SV=1)

HSP 1 Score: 2149.8 bits (5569), Expect = 0.0e+00
Identity = 1053/1053 (100.00%), Postives = 1053/1053 (100.00%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN
Sbjct: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG
Sbjct: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY
Sbjct: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS
Sbjct: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV
Sbjct: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL 1054
            AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL
Sbjct: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSARFPNL 1053

BLAST of Moc06g07730 vs. ExPASy TrEMBL
Match: A0A6J1JAS5 (subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3 SV=1)

HSP 1 Score: 1974.1 bits (5113), Expect = 0.0e+00
Identity = 964/1054 (91.46%), Postives = 1005/1054 (95.35%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MI  S+SSS +SF +IFVPIFISIS+FQF PSSD  V+QTLTRNYS  DPFTSKN++DGN
Sbjct: 1    MIGFSISSSSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            +VS+KNYIVRFVQYREAKEHR YLESRIRSG W+WIER+NPASKYPT+FGLVSIEDS   
Sbjct: 61   RVSQKNYIVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVR 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            EL+++IEE ELVKDVNVDAS+VR +L E GGR+GAFVDGKKRPGKIFTSMSF EGGGERY
Sbjct: 121  ELVEEIEEFELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEGGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHLSMQRSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLSMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTW+PSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWNPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPS GEKNRR STCVL LKLKVV TPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSRGEKNRRISTCVLQLKLKVVSTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVM+TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPR GYVHSFPFIDSSESGAAQ+ILTS ++KADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRDGYVHSFPFIDSSESGAAQSILTSSLSKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLL E GEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK +S
Sbjct: 841  ILGLL-EAGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSDVNFS+YS V GKELIC SDSRF+ WGTKGY++Q RGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            +IDLGR LNST E+S M PPKLT ++RSD YGNRYL+LFY DEPDMPLIVPNHWLVPAVV
Sbjct: 961  LIDLGRDLNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGSA-RFPNL 1054
            AV GLLLLLSFWRIRQ+RRRRRRGSGSA RF NL
Sbjct: 1021 AVAGLLLLLSFWRIRQKRRRRRRGSGSAPRFSNL 1053

BLAST of Moc06g07730 vs. ExPASy TrEMBL
Match: A0A6J1H8D8 (subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE=3 SV=1)

HSP 1 Score: 1971.8 bits (5107), Expect = 0.0e+00
Identity = 963/1054 (91.37%), Postives = 1004/1054 (95.26%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            MI  S+SSS +SF +IFVPIFISIS+FQF PSSD  V+QTLTRNYS  DPFTSKN++DGN
Sbjct: 1    MIGYSISSSSISFFVIFVPIFISISVFQFNPSSDHTVFQTLTRNYSPNDPFTSKNVTDGN 60

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            +VS+KNYIVRFVQYREAKEHR YLESRIRSG W+WIER+NPASKYPT+FGLVSIEDS   
Sbjct: 61   RVSQKNYIVRFVQYREAKEHRFYLESRIRSGGWKWIERRNPASKYPTDFGLVSIEDSVVM 120

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
            EL+++IEELELVKDVNVDAS+VR +L E GGR+GAFVDGKKRPGKIFTSMSF E GGERY
Sbjct: 121  ELVEEIEELELVKDVNVDASHVRGLLAEGGGRIGAFVDGKKRPGKIFTSMSFNEEGGERY 180

Query: 181  TAISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIK 240
            TAISNASNRWGRHL MQRSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIK
Sbjct: 181  TAISNASNRWGRHLLMQRSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIK 240

Query: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300
            ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD
Sbjct: 241  ERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLD 300

Query: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360
            AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD
Sbjct: 301  AFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPAD 360

Query: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLS 420
            QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLS
Sbjct: 361  QSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLS 420

Query: 421  GTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDL 480
            GTSVASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDL
Sbjct: 421  GTSVASPVVAGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDL 480

Query: 481  LESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540
            LESYEILKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYV
Sbjct: 481  LESYEILKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYV 540

Query: 541  EGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600
            EGQPTW+PSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS
Sbjct: 541  EGQPTWNPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYS 600

Query: 601  PPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILD 660
            PPS GEKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILD
Sbjct: 601  PPSRGEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILD 660

Query: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLR 720
            WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLR
Sbjct: 661  WHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLR 720

Query: 721  DDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780
            DDVM+TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG
Sbjct: 721  DDVMITGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFG 780

Query: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFP 840
            DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQ+ILTS ++KADFP
Sbjct: 781  DKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFP 840

Query: 841  ILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDS 900
            ILGLL E  EGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK +S
Sbjct: 841  ILGLL-EAAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNS 900

Query: 901  PLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFP 960
            PLYLEDNKLPSRRSD NFS+YS V GKELIC SDSRF+ WGTKGY++Q RGRNRRLPGFP
Sbjct: 901  PLYLEDNKLPSRRSDANFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFP 960

Query: 961  IIDLGRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVV 1020
            +IDLGR LNST E+S M PPKLT ++RSD YGNRYL+LFY DEPDMPLIVPNHWLVPAVV
Sbjct: 961  LIDLGRDLNSTPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVV 1020

Query: 1021 AVTGLLLLLSFWRIRQRRRRRRRGSGS-ARFPNL 1054
            AV GLLLLLSFWRIRQ+RRRRRRGSGS ARF NL
Sbjct: 1021 AVAGLLLLLSFWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of Moc06g07730 vs. ExPASy TrEMBL
Match: A0A0A0LTP5 (Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G250160 PE=3 SV=1)

HSP 1 Score: 1959.9 bits (5076), Expect = 0.0e+00
Identity = 952/1049 (90.75%), Postives = 1001/1049 (95.42%), Query Frame = 0

Query: 6    VSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGNQVSRK 65
            ++SS +SF+ IF+PIFISI LFQFKPSSD     TLT NYS+ DP    NL+ GNQV RK
Sbjct: 2    IASSSISFIAIFLPIFISIYLFQFKPSSD----HTLTPNYSTFDPSPFNNLTHGNQVLRK 61

Query: 66   -NYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARGELIK 125
             NYIVRF+ YR+AK+HR YLES +RSG WEWI+R+NPASKYPT+FGLVSIEDS RGELI+
Sbjct: 62   QNYIVRFLHYRKAKDHRFYLESHVRSGGWEWIQRRNPASKYPTDFGLVSIEDSVRGELIE 121

Query: 126  DIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAIS 185
            +IEELELVKDVNVDAS+VR +LTE+GGRVGAFVDGKKRPGKIFTSMSFKEGGGE YTAI+
Sbjct: 122  EIEELELVKDVNVDASHVRGLLTEDGGRVGAFVDGKKRPGKIFTSMSFKEGGGEHYTAIT 181

Query: 186  NASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIKERTN 245
            NASNRWGRHLSM+RSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIKERTN
Sbjct: 182  NASNRWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTN 241

Query: 246  WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 305
            WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY
Sbjct: 242  WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 301

Query: 306  AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV 365
            AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV
Sbjct: 302  AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV 361

Query: 366  IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV 425
            IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV
Sbjct: 362  IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV 421

Query: 426  ASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDLLESY 485
            ASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDLLESY
Sbjct: 422  ASPVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESY 481

Query: 486  EILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP 545
            E+LKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP
Sbjct: 482  EVLKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP 541

Query: 546  TWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSS 605
            TWHPSDEEGNLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPS 
Sbjct: 542  TWHPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSR 601

Query: 606  GEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGD 665
            GEKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILDWHGD
Sbjct: 602  GEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGD 661

Query: 666  HLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLRDDVM 725
            HLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLRDDVM
Sbjct: 662  HLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM 721

Query: 726  VTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL 785
             TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL
Sbjct: 722  TTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL 781

Query: 786  NGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFPILGL 845
            NGDFSIDGEQSRYASGTDIVRFP+GGY+HSFPF+DSSESGAAQ+ILTS M+KADFPILGL
Sbjct: 782  NGDFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGL 841

Query: 846  LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDSPLYL 905
            L E GEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTK SK +SPLYL
Sbjct: 842  L-EAGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYL 901

Query: 906  EDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFPIIDL 965
            ED+KLPSRRSDVNFS+YS V  KELICRSDSRF+ WGTKGY+IQVRGRNRRLPGFP+IDL
Sbjct: 902  EDSKLPSRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDL 961

Query: 966  GRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVVAVTG 1025
            GRGLNSTSE SSM PPK +S++RSD+YGNRYL+LFY DEPDMPLIVPNHWLVPAVVA+TG
Sbjct: 962  GRGLNSTSEGSSMGPPKSSSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTG 1021

Query: 1026 LLLLLSFWRIRQRRRRRRRGSGSARFPNL 1054
            L LLLSFWRIRQ+RRRRRRGSGSARF N+
Sbjct: 1022 LFLLLSFWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of Moc06g07730 vs. ExPASy TrEMBL
Match: A0A1S3CJL6 (subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=1)

HSP 1 Score: 1952.6 bits (5057), Expect = 0.0e+00
Identity = 952/1049 (90.75%), Postives = 1001/1049 (95.42%), Query Frame = 0

Query: 6    VSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGNQVSRK 65
            ++SS VSF+ IF+PIFISISLFQFKP++   ++QTLT NYS+ DP    NL+  N V RK
Sbjct: 2    IASSSVSFIAIFLPIFISISLFQFKPNT---LFQTLTPNYSTFDPSPFNNLTHRNSVLRK 61

Query: 66   -NYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARGELIK 125
             NYIVRF+QYR+AK+HR YLESR+RSG WEWI+R+NPASKYPT+FGLVSIEDS RGELI+
Sbjct: 62   QNYIVRFLQYRQAKDHRFYLESRVRSGGWEWIQRRNPASKYPTDFGLVSIEDSVRGELIE 121

Query: 126  DIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAIS 185
            +IEELELVKDVNVDAS+VR +LTE+ GRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAI+
Sbjct: 122  EIEELELVKDVNVDASHVRGLLTEDAGRVGAFVDGKKRPGKIFTSMSFKEGGGERYTAIT 181

Query: 186  NASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNIKERTN 245
            NASNRWGRHLSM+RSQVTSLFGA+SLWAKGYTGSKV+MAIFDTGIRANHPHFRNIKERTN
Sbjct: 182  NASNRWGRHLSMERSQVTSLFGADSLWAKGYTGSKVKMAIFDTGIRANHPHFRNIKERTN 241

Query: 246  WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 305
            WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY
Sbjct: 242  WTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 301

Query: 306  AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV 365
            AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV
Sbjct: 302  AIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDV 361

Query: 366  IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV 425
            IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV
Sbjct: 362  IGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSV 421

Query: 426  ASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVDLLESY 485
            ASPVVAGVVCLLVSVIPESQR+ ILNPASMKQALVEGAAKLAGPN+YEQGAGRVDLLESY
Sbjct: 422  ASPVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESY 481

Query: 486  EILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP 545
            E+LKSYQPRASIFP VLDYTDCPY WPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP
Sbjct: 482  EVLKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQP 541

Query: 546  TWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSS 605
            TWHPSDEE NLLSIHFTYS+VIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPS 
Sbjct: 542  TWHPSDEEANLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSR 601

Query: 606  GEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGD 665
            GEKNRR STCVL LKLKVVPTPPRSKRILWDQFH+IKYPPGYIPRDSLDVRNDILDWHGD
Sbjct: 602  GEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGD 661

Query: 666  HLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKLRDDVM 725
            HLHTNFHIMFNMLRDAGYYVETLGSPLTCFD+ QYGTLLLVDLEDEYFKEEIEKLRDDVM
Sbjct: 662  HLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM 721

Query: 726  VTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL 785
             TGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL
Sbjct: 722  TTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKIL 781

Query: 786  NGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADFPILGL 845
            NGDFSIDGEQSRYASGTDIVRFP+GGYVHSFPF+DSSESGAAQ+ILTS M+KADF ILGL
Sbjct: 782  NGDFSIDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFSILGL 841

Query: 846  LEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLDSPLYL 905
            L E  EGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTK SK  +PLYL
Sbjct: 842  L-EAAEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRKTPLYL 901

Query: 906  EDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGFPIIDL 965
            ED+KLPSRRSDVNFS+YS V GKELICRSDSRF+ WGTKGY+ QVRGRNRRLPGFP+IDL
Sbjct: 902  EDSKLPSRRSDVNFSLYSAVAGKELICRSDSRFEVWGTKGYSTQVRGRNRRLPGFPVIDL 961

Query: 966  GRGLNSTSETSSMRPPKLTSENRSDSYGNRYLNLFYGDEPDMPLIVPNHWLVPAVVAVTG 1025
            GRGLNSTSE SSM PPK TS++RSD+YGNRYL+LFY DEPDMPL VPNHWLVPAVVA+TG
Sbjct: 962  GRGLNSTSE-SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLSVPNHWLVPAVVALTG 1021

Query: 1026 LLLLLSFWRIRQRRRRRRRGSGSARFPNL 1054
            LLLLLSFWRIRQ+RRRRRRGSGSARF N+
Sbjct: 1022 LLLLLSFWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of Moc06g07730 vs. TAIR 10
Match: AT5G19660.1 (SITE-1 protease )

HSP 1 Score: 1595.1 bits (4129), Expect = 0.0e+00
Identity = 779/1054 (73.91%), Postives = 892/1054 (84.63%), Query Frame = 0

Query: 1    MISSSVSSSYVSFVLIFVPIFISISLFQFKPSSDPIVYQTLTRNYSSVDPFTSKNLSDGN 60
            ++  + S  Y S +++   +F+S+SLF  +PS       T      +++P     L   N
Sbjct: 3    VLGEASSYPYRSCIIV---VFLSVSLFWLRPS-------TYHPQQQNLNPENVTRLESEN 62

Query: 61   QVSRKNYIVRFVQYREAKEHRSYLESRIRSGLWEWIERKNPASKYPTEFGLVSIEDSARG 120
            + ++ NYI+RF QY+ AK+HR YLES++RSG W WIER NPA+KYPT+FG++ IE+S + 
Sbjct: 63   E-TKTNYIIRFKQYKPAKDHRIYLESKVRSGGWGWIERINPATKYPTDFGVLWIEESGKE 122

Query: 121  ELIKDIEELELVKDVNVDASYVRDVLTENGGRVGAFVDGKKRPGKIFTSMSFKEGGGERY 180
             ++ +IE LE+VKDVNV+  Y R +L       G+F DGKKRPGKIFTSMSF+EG     
Sbjct: 123  AVVGEIERLEMVKDVNVEFKYQRVLLG------GSFPDGKKRPGKIFTSMSFEEGTESSP 182

Query: 181  TA-ISNASNRWGRHLSMQRSQVTSLFGAESLWAKGYTGSKVRMAIFDTGIRANHPHFRNI 240
             A  SN +  W RHL  Q++QVTS+FGA+ LW KGYTG+KV+MAIFDTGIRA+HPHFR I
Sbjct: 183  MADTSNTTLNWSRHLLAQKTQVTSMFGADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKI 242

Query: 241  KERTNWTNEDTLNDNLGHGTFVAGVIAGGDEECLGFAPDTEIYAFRVFTDAQVSYTSWFL 300
            KERTNWTNEDTLNDNLGHGTFVAGVIAG + ECLGFA DTEIYAFRVFTDAQVSYTSWFL
Sbjct: 243  KERTNWTNEDTLNDNLGHGTFVAGVIAGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFL 302

Query: 301  DAFNYAIATNMDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPA 360
            DAFNYAIAT+MDVLNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPA
Sbjct: 303  DAFNYAIATDMDVLNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPA 362

Query: 361  DQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSL 420
            DQSDVIGVGGID +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSL
Sbjct: 363  DQSDVIGVGGIDNDDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSL 422

Query: 421  SGTSVASPVVAGVVCLLVSVIPESQRENILNPASMKQALVEGAAKLAGPNVYEQGAGRVD 480
            SGTSVASPVVAG+VCLLVSVIPE++R+++LNPASMKQALVEGAAKL+GPN+YEQGAGRVD
Sbjct: 423  SGTSVASPVVAGIVCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVD 482

Query: 481  LLESYEILKSYQPRASIFPNVLDYTDCPYMWPFCRQPLYAGAMPIIFNATILNGMGVIGY 540
            LLESYEILKSY PRASIFP++LDY DCPY WPFCRQPLYAGAMPIIFN TILNGMGVIGY
Sbjct: 483  LLESYEILKSYHPRASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGY 542

Query: 541  VEGQPTWHPSDEEGNLLSIHFTYSQVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVY 600
            +E  PTWHP++EEGNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VY
Sbjct: 543  IESPPTWHPANEEGNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVY 602

Query: 601  SPPSSGEKNRRTSTCVLHLKLKVVPTPPRSKRILWDQFHSIKYPPGYIPRDSLDVRNDIL 660
            SPP+SGE   R STC L LKLKV+PTPPR+KRILWDQFHSIKYPPGYIPRDSLDVRNDIL
Sbjct: 603  SPPASGESGPRRSTCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDIL 662

Query: 661  DWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDSLQYGTLLLVDLEDEYFKEEIEKL 720
            DWHGDHLHTNFHIM+NMLRDAGYY+ETLGSPLTCFD+ QYGTLL+VDLED+YF EEIEKL
Sbjct: 663  DWHGDHLHTNFHIMYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKL 722

Query: 721  RDDVMVTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAF 780
            RDDV+ TGLGL VF+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAF
Sbjct: 723  RDDVINTGLGLVVFAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAF 782

Query: 781  GDKILNGDFSIDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQNILTSGMTKADF 840
            GDKILNGDFSIDGEQSRYASGT+IVRFP GG++H+FP +DSSESGA QN+L +  +K D 
Sbjct: 783  GDKILNGDFSIDGEQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDP 842

Query: 841  PILGLLEEVGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKSSKLD 900
             +LGLL E+GEGR+ VYGDSNCLDSSHMVTNCYWLL+K+LDF+S NI+DPVLF+K +K  
Sbjct: 843  AVLGLL-EIGEGRVGVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRY 902

Query: 901  SPLYLEDNKLPSRRSDVNFSMYSVVVGKELICRSDSRFDTWGTKGYNIQVRGRNRRLPGF 960
            SP+ +++ +LPSRR+DVNFS YS V+GKELIC SDSRF+ WGTKGYN+ VRGRNRRLPG+
Sbjct: 903  SPVIIDEKQLPSRRTDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGY 962

Query: 961  PIIDLGRGLNSTSETSSMRPPKLTSENR----SDSYGNRYLNLFYGDEPDMPLIVPNHWL 1020
              IDLGRGLN T E  S RP +  S       S S       LF  DE DMP +VP  W+
Sbjct: 963  HGIDLGRGLNFTVE--SKRPTRWRSAKEGGELSSSRSKSLGGLFNRDEIDMPFLVPTRWI 1022

Query: 1021 VPAVVAVTGLLLLLSFWRIRQRRRRRRRGSGSAR 1050
            V A V  +G+L+LLS WRIRQ+R RRRR SGS R
Sbjct: 1023 VLAGVVASGVLVLLSIWRIRQKRGRRRRASGSNR 1036

BLAST of Moc06g07730 vs. TAIR 10
Match: AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 43/138 (31.16%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 250 TLNDNLGHGTFVAGVIAGGDEE-----------CLGFAPDTEIYAFRVFTDAQVSYTSWF 309
           T  D +GHG+ V+  IAG   E             G + +  I  ++V      + +S  
Sbjct: 203 TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSS-I 262

Query: 310 LDAFNYAIATNMDVLNLSIGGPDY--LDL---PFVEKIWEITANNIIMVSAIGNDGPLYG 369
           L AF+ AIA  +DVL+LS+G P Y  +DL   P     +      I+++ + GNDGP  G
Sbjct: 263 LAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGG 322

Query: 370 TLNNPADQSDVIGVGGID 372
           T+ N A     +    ID
Sbjct: 323 TVTNTAPWIMTVAANTID 339

BLAST of Moc06g07730 vs. TAIR 10
Match: AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 43/138 (31.16%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 250 TLNDNLGHGTFVAGVIAGGDEE-----------CLGFAPDTEIYAFRVFTDAQVSYTSWF 309
           T  D +GHG+ V+  IAG   E             G + +  I  ++V      + +S  
Sbjct: 164 TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSS-I 223

Query: 310 LDAFNYAIATNMDVLNLSIGGPDY--LDL---PFVEKIWEITANNIIMVSAIGNDGPLYG 369
           L AF+ AIA  +DVL+LS+G P Y  +DL   P     +      I+++ + GNDGP  G
Sbjct: 224 LAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGG 283

Query: 370 TLNNPADQSDVIGVGGID 372
           T+ N A     +    ID
Sbjct: 284 TVTNTAPWIMTVAANTID 300

BLAST of Moc06g07730 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 44/133 (33.08%), Postives = 61/133 (45.86%), Query Frame = 0

Query: 253 DNLGHGTFVAGVIAG-----------GDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDA 312
           D+ GHGT  A + AG                 G AP   + A++V  ++   Y S  L A
Sbjct: 206 DSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSG-CYDSDILAA 265

Query: 313 FNYAIATNMDVLNLSIGG---PDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNP 372
           F+ A+A  +DV++LS+GG   P YLD   +     I     +  SA GN GP   T+ N 
Sbjct: 266 FDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASA-GNGGPGALTVTNV 325

BLAST of Moc06g07730 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 49.7 bits (117), Expect = 1.7e-05
Identity = 40/135 (29.63%), Postives = 57/135 (42.22%), Query Frame = 0

Query: 256 GHGTFVAGVIAG-----------GDEECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNY 315
           GHGT  +   AG                 G AP   I A++V         S  L AF+ 
Sbjct: 204 GHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDA 263

Query: 316 AIATNMDVLNLSIGG------PDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNP 374
           A+   +DV+++SIGG      P YLD P     +   +  I + S+ GN+GP   ++ N 
Sbjct: 264 AVRDGVDVISISIGGGDGITSPYYLD-PIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNL 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144866.10.0e+00100.00subtilisin-like protease SBT6.1 isoform X1 [Momordica charantia][more]
XP_038879333.10.0e+0091.89subtilisin-like protease SBT6.1 [Benincasa hispida][more]
XP_022987592.10.0e+0091.46subtilisin-like protease SBT6.1 [Cucurbita maxima][more]
KAG6589917.10.0e+0091.46Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022960716.10.0e+0091.37subtilisin-like protease SBT6.1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q0WUG60.0e+0073.91Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 S... [more]
Q147033.9e-25750.28Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=M... [more]
Q9Z2A84.3e-25650.88Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=100... [more]
Q9WTZ31.3e-25551.12Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=1011... [more]
Q9WTZ28.2e-25550.76Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1CUP30.0e+00100.00subtilisin-like protease SBT6.1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1JAS50.0e+0091.46subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3... [more]
A0A6J1H8D80.0e+0091.37subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE... [more]
A0A0A0LTP50.0e+0090.75Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G25016... [more]
A0A1S3CJL60.0e+0090.75subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G19660.10.0e+0073.91SITE-1 protease [more]
AT1G20160.13.1e-0731.16Subtilisin-like serine endopeptidase family protein [more]
AT1G20160.23.1e-0731.16Subtilisin-like serine endopeptidase family protein [more]
AT3G14240.13.1e-0733.08Subtilase family protein [more]
AT4G34980.11.7e-0529.63subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 217..236
score: 36.62
coord: 253..266
score: 52.92
coord: 420..436
score: 51.94
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 186..483
e-value: 6.0E-70
score: 237.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 202..489
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 217..469
e-value: 1.6E-43
score: 149.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 969..988
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 62..868
NoneNo IPR availablePANTHERPTHR43806:SF7MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASEcoord: 62..868
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 193..487
score: 34.323597
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 257..267
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 421..431
IPR034185Site-1 peptidase catalytic domainCDDcd07479Peptidases_S8_SKI-1_likecoord: 211..467
e-value: 0.0
score: 531.644

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g07730.1Moc06g07730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity