Moc03g29750 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g29750
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMalate synthase
Locationchr3: 21235027 .. 21237418 (+)
RNA-Seq ExpressionMoc03g29750
SyntenyMoc03g29750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCGCTTGGAATGTATTCTGAACCAACGAGGAAGACTAGCAGAGGCTATGACGTTCCAGAGGGAGTAGATATTCGGGGGCGTTACGATGAAGAATTTGCCAAGATCCTTAACAAGGAAGCCCTGCTATTTGTGGCTGATTTGCAGAGGACTTTCAGAAACCATATCAGGTATTCGATGGAATGCCGCAGAGAAGCCAAGAGGAGATATAATGAAGGGGCGTTGCCTGGGTTTGATCCGGCGACCAAATATATAAGGGAATCGGAGTGGACATGTGCATCAGTCCCGCCGGCAGTTGCTGACCGGAGGGTGGAGATCACCGGACCTGTGGAGCGGAAGATGATCATCAACGCACTCAATTCTGGAGCCAAAGTTTTCATGGTCCGTTCTCTCTTCCTTTCTCTACTCAAGAATTTGTTTTCGTTCCTTGGGAGAATGTAATTTGATGATCTGACAAAAACATATTAAATCAATCAACTGTTGGAAAACATCAAAACAGGCGGACTTTGAAGATGCACTGTCACCGAACTGGGAGAATCTGATGAGGGGGCAAATTAATCTGAAGGATGCAGTAGATGGGACTATAAGCTTCCATGATAAAGCCAGGAACAGGGTTTATAAGCTGAATGATCAGACGGCCAAGCTCTTCGTCCGCCCTCGAGGTTGGCACTTGCCTGAGGCTCACATATTCATCGACGGCGAGCCTGCCACTGGTTGTCTTGTGGACTTTGGGCTCTACTTTTACCACAACCATGCCAATTTCCGGCGCTCTCAAGGTCAAGGCTCTGGCCCTTTCTTCTATCTCCCCAAAATGGAGCACTCCAGGTAAAAGTAGAACTATCATTTTAACCATCCAAATCTTCTTTTATAATCAATTGGGCATGTCGTTTGAGAAATTGAATTCTGTTTGGTGTTTGCAATGAGAATCTCTCTTAGGTGATGCGTAAAGATCTGGTAGAGATTGATAATTCATTAGGAAAGCAGGTTAGATAGATAGCAATATGTAGAGAATGAAAACCCATGGTTAGAGGAGATTGAGAGCAAGTACAAAAGAGGAGAGTTTTAGAATTGAGGCTACAGAATTTGAAACGAAATAAAATCTTGATGTGCTTATTGAAAAATATTATTGGCCCGATCCCTTTGTTATCATCAGAAACTTCCAAACAAAAAAAATCTCTTTACAAGTCACATTGAGGGCTTTTTATCTTCGGTCATAATAGATTCCGGTGATCACATTATAACATCATTACAAGCTATGTGACATGTCATCTGCGATTCTGCAGGGAAGCTAAAATATGGAACAGTGTATTTGAGAGGGCAGAGAATATTGCGGGGATAGAGAGGGGCAGTATCAGGGCCACTGTGCTAATTGAAACACTTCCAGCAGTGTTTCAAATGGATGAAATACTCTATGAGCTGAGGGATCACTCTGTGGGATTAAACTGCGGTAGATGGGATTACATATTCAGCTATGTCAAGACCTTTCAGGCTCACCCAGATCGCCTCTTACCCGACAGGATTCTAGTTGGCATGACTCAACACTTCATGAGGAGTTATTCTGATCTCCTTATCAGGACTTGTCACAGGCGTGGTGTGCACGCCATGGGAGGCATGGTATGTATAAATGCACAATCTCACATCCAATTCGAAAGATAAATTAGCATGCTACCACCTTTCTAGCTCTTAAAAACCGGTACTTCACATCTAGAGTATTGACAGTAGCATTTTACAGGCTGCTCAAATTCCAATCAGAGATGATCCAAAGGCAAATGAGGCAGCGCTGGAGCTAGTAAGGAAGGACAAATTGAGAGAAGTAAAGGCAGGACATGATGGAACATGGGCAGCACACCCAGGACTGATCCCAGCTTGCATGGAAGTGTTCACCAACAACATGGGGAATGCCCCCAACCAGATTCGATCTGCGAGACGAGAAGATGCTGCAAACCTGACTGAAGAAGACCTCTTACAACGACCAAGGGGTGTCCGCACAATGGAAGGGCTCCGCTTGAACACCCGAGTCGGGATTCAGTACTTGGCAGCATGGCTGACTGGGACTGGCTCTGTGCCTCTCTACAACCTTATGGAAGATGCAGCCACAGCAGAGATCAGCAGAGTTCAAAACTGGCAATGGCTGAAGTATGGAGTGGAGTTGGATGGAGATGGCCTTGGTGTGAGAGTGAACAAGGAACTGTTTGGAAGAGTTGTGGAAGAAGAGATGGATAGGATTGAAAGAGAAGTTGGGAAAGAGAAATTCAGAAGGGGAATGTTCAAAGAGGCTGGCAAAATGTTCACAAGGCAATGCACAGCTCCAAGTTTAGATGATTTTCTGACCTTAGATGCCTACAACCGCATTGTGCTTCATCATCCCAGGGAATTGTCCAGGCTCTGA

mRNA sequence

ATGGGATCGCTTGGAATGTATTCTGAACCAACGAGGAAGACTAGCAGAGGCTATGACGTTCCAGAGGGAGTAGATATTCGGGGGCGTTACGATGAAGAATTTGCCAAGATCCTTAACAAGGAAGCCCTGCTATTTGTGGCTGATTTGCAGAGGACTTTCAGAAACCATATCAGGTATTCGATGGAATGCCGCAGAGAAGCCAAGAGGAGATATAATGAAGGGGCGTTGCCTGGGTTTGATCCGGCGACCAAATATATAAGGGAATCGGAGTGGACATGTGCATCAGTCCCGCCGGCAGTTGCTGACCGGAGGGTGGAGATCACCGGACCTGTGGAGCGGAAGATGATCATCAACGCACTCAATTCTGGAGCCAAAGTTTTCATGGCGGACTTTGAAGATGCACTGTCACCGAACTGGGAGAATCTGATGAGGGGGCAAATTAATCTGAAGGATGCAGTAGATGGGACTATAAGCTTCCATGATAAAGCCAGGAACAGGGTTTATAAGCTGAATGATCAGACGGCCAAGCTCTTCGTCCGCCCTCGAGGTTGGCACTTGCCTGAGGCTCACATATTCATCGACGGCGAGCCTGCCACTGGTTGTCTTGTGGACTTTGGGCTCTACTTTTACCACAACCATGCCAATTTCCGGCGCTCTCAAGGTCAAGGCTCTGGCCCTTTCTTCTATCTCCCCAAAATGGAGCACTCCAGGGAAGCTAAAATATGGAACAGTGTATTTGAGAGGGCAGAGAATATTGCGGGGATAGAGAGGGGCAGTATCAGGGCCACTGTGCTAATTGAAACACTTCCAGCAGTGTTTCAAATGGATGAAATACTCTATGAGCTGAGGGATCACTCTGTGGGATTAAACTGCGGTAGATGGGATTACATATTCAGCTATGTCAAGACCTTTCAGGCTCACCCAGATCGCCTCTTACCCGACAGGATTCTAGTTGGCATGACTCAACACTTCATGAGGAGTTATTCTGATCTCCTTATCAGGACTTGTCACAGGCGTGGTGTGCACGCCATGGGAGGCATGGCTGCTCAAATTCCAATCAGAGATGATCCAAAGGCAAATGAGGCAGCGCTGGAGCTAGTAAGGAAGGACAAATTGAGAGAAGTAAAGGCAGGACATGATGGAACATGGGCAGCACACCCAGGACTGATCCCAGCTTGCATGGAAGTGTTCACCAACAACATGGGGAATGCCCCCAACCAGATTCGATCTGCGAGACGAGAAGATGCTGCAAACCTGACTGAAGAAGACCTCTTACAACGACCAAGGGGTGTCCGCACAATGGAAGGGCTCCGCTTGAACACCCGAGTCGGGATTCAGTACTTGGCAGCATGGCTGACTGGGACTGGCTCTGTGCCTCTCTACAACCTTATGGAAGATGCAGCCACAGCAGAGATCAGCAGAGTTCAAAACTGGCAATGGCTGAAGTATGGAGTGGAGTTGGATGGAGATGGCCTTGGTGTGAGAGTGAACAAGGAACTGTTTGGAAGAGTTGTGGAAGAAGAGATGGATAGGATTGAAAGAGAAGTTGGGAAAGAGAAATTCAGAAGGGGAATGTTCAAAGAGGCTGGCAAAATGTTCACAAGGCAATGCACAGCTCCAAGTTTAGATGATTTTCTGACCTTAGATGCCTACAACCGCATTGTGCTTCATCATCCCAGGGAATTGTCCAGGCTCTGA

Coding sequence (CDS)

ATGGGATCGCTTGGAATGTATTCTGAACCAACGAGGAAGACTAGCAGAGGCTATGACGTTCCAGAGGGAGTAGATATTCGGGGGCGTTACGATGAAGAATTTGCCAAGATCCTTAACAAGGAAGCCCTGCTATTTGTGGCTGATTTGCAGAGGACTTTCAGAAACCATATCAGGTATTCGATGGAATGCCGCAGAGAAGCCAAGAGGAGATATAATGAAGGGGCGTTGCCTGGGTTTGATCCGGCGACCAAATATATAAGGGAATCGGAGTGGACATGTGCATCAGTCCCGCCGGCAGTTGCTGACCGGAGGGTGGAGATCACCGGACCTGTGGAGCGGAAGATGATCATCAACGCACTCAATTCTGGAGCCAAAGTTTTCATGGCGGACTTTGAAGATGCACTGTCACCGAACTGGGAGAATCTGATGAGGGGGCAAATTAATCTGAAGGATGCAGTAGATGGGACTATAAGCTTCCATGATAAAGCCAGGAACAGGGTTTATAAGCTGAATGATCAGACGGCCAAGCTCTTCGTCCGCCCTCGAGGTTGGCACTTGCCTGAGGCTCACATATTCATCGACGGCGAGCCTGCCACTGGTTGTCTTGTGGACTTTGGGCTCTACTTTTACCACAACCATGCCAATTTCCGGCGCTCTCAAGGTCAAGGCTCTGGCCCTTTCTTCTATCTCCCCAAAATGGAGCACTCCAGGGAAGCTAAAATATGGAACAGTGTATTTGAGAGGGCAGAGAATATTGCGGGGATAGAGAGGGGCAGTATCAGGGCCACTGTGCTAATTGAAACACTTCCAGCAGTGTTTCAAATGGATGAAATACTCTATGAGCTGAGGGATCACTCTGTGGGATTAAACTGCGGTAGATGGGATTACATATTCAGCTATGTCAAGACCTTTCAGGCTCACCCAGATCGCCTCTTACCCGACAGGATTCTAGTTGGCATGACTCAACACTTCATGAGGAGTTATTCTGATCTCCTTATCAGGACTTGTCACAGGCGTGGTGTGCACGCCATGGGAGGCATGGCTGCTCAAATTCCAATCAGAGATGATCCAAAGGCAAATGAGGCAGCGCTGGAGCTAGTAAGGAAGGACAAATTGAGAGAAGTAAAGGCAGGACATGATGGAACATGGGCAGCACACCCAGGACTGATCCCAGCTTGCATGGAAGTGTTCACCAACAACATGGGGAATGCCCCCAACCAGATTCGATCTGCGAGACGAGAAGATGCTGCAAACCTGACTGAAGAAGACCTCTTACAACGACCAAGGGGTGTCCGCACAATGGAAGGGCTCCGCTTGAACACCCGAGTCGGGATTCAGTACTTGGCAGCATGGCTGACTGGGACTGGCTCTGTGCCTCTCTACAACCTTATGGAAGATGCAGCCACAGCAGAGATCAGCAGAGTTCAAAACTGGCAATGGCTGAAGTATGGAGTGGAGTTGGATGGAGATGGCCTTGGTGTGAGAGTGAACAAGGAACTGTTTGGAAGAGTTGTGGAAGAAGAGATGGATAGGATTGAAAGAGAAGTTGGGAAAGAGAAATTCAGAAGGGGAATGTTCAAAGAGGCTGGCAAAATGTTCACAAGGCAATGCACAGCTCCAAGTTTAGATGATTTTCTGACCTTAGATGCCTACAACCGCATTGTGCTTCATCATCCCAGGGAATTGTCCAGGCTCTGA

Protein sequence

MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAPSLDDFLTLDAYNRIVLHHPRELSRL
Homology
[Close]
BLAST of Moc03g29750 vs. NCBI nr
Match: XP_022137538.1 (malate synthase, glyoxysomal [Momordica charantia])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0 

Query: 1   MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS 60
           MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS
Sbjct: 1   MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS 60

Query: 61  MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL 120
           MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL
Sbjct: 61  MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL 120

Query: 121 NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR 180
           NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR
Sbjct: 121 NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR 180

Query: 181 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK 240
           PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK
Sbjct: 181 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK 240

Query: 241 IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY 300
           IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY
Sbjct: 241 IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY 300

Query: 301 VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN 360
           VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN
Sbjct: 301 VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN 360

Query: 361 EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT 420
           EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT
Sbjct: 361 EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT 420

Query: 421 EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW 480
           EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW
Sbjct: 421 EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW 480

Query: 481 LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP 540
           LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP
Sbjct: 481 LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP 540

Query: 541 SLDDFLTLDAYNRIVLHHPRELSRL 566
           SLDDFLTLDAYNRIVLHHPRELSRL
Sbjct: 541 SLDDFLTLDAYNRIVLHHPRELSRL 565

[Close]
BLAST of Moc03g29750 vs. NCBI nr
Match: XP_023519701.1 (malate synthase, glyoxysomal [Cucurbita pepo subsp. pepo] >XP_023519702.1 malate synthase, glyoxysomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 536/567 (94.53%), Postives = 554/567 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+ILNKEAL+FVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILNKEALVFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCA VPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCAPVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAHPDRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHPDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LT +DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTADDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 567

[Close]
BLAST of Moc03g29750 vs. NCBI nr
Match: XP_023000792.1 (malate synthase, glyoxysomal [Cucurbita maxima] >XP_023000793.1 malate synthase, glyoxysomal [Cucurbita maxima] >ADP06653.1 malate synthase [Cucurbita maxima])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 536/567 (94.53%), Postives = 554/567 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+ILNKEALLFVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILNKEALLFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAH DRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHLDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFTN+MGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTNSMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LTE+DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTEDDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELF RVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 567

[Close]
BLAST of Moc03g29750 vs. NCBI nr
Match: KAG7019729.1 (hypothetical protein SDJN02_18692, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 554/567 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+IL+KEALLFVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILSKEALLFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCA VPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCAPVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAHPDRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHPDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFT+NMGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTHNMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LT +DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTADDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 567

[Close]
BLAST of Moc03g29750 vs. NCBI nr
Match: KAG6584139.1 (hypothetical protein SDJN03_20071, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 554/567 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+IL+KEALLFVADLQRTFRNHIR
Sbjct: 65  MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILSKEALLFVADLQRTFRNHIR 124

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCA VPPAVADRRVEITGPVERKMIIN
Sbjct: 125 YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCAPVPPAVADRRVEITGPVERKMIIN 184

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 185 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 244

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 245 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 304

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 305 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 364

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAHPDRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 365 SYVKTFQAHPDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 424

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFT+NMGNAPNQIRSARR+DAAN
Sbjct: 425 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTHNMGNAPNQIRSARRDDAAN 484

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LT +DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 485 LTADDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 544

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 545 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 604

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 605 APTLDDFLTLDAYNHIVIHHPRELSRL 631

[Close]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match: P08216 (Malate synthase, glyoxysomal OS=Cucumis sativus OX=3659 PE=2 SV=2)

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 532/568 (93.66%), Postives = 553/568 (97.36%), Query Frame = 0 

Query: 1   MGSLGMYSEP--TRK-TSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI 60
           MGSLGMYSE   T+K +SRGYDVPEGVDIRGRYDEEFAKILNKEALLF+ADLQRTFRNHI
Sbjct: 1   MGSLGMYSESGLTKKGSSRGYDVPEGVDIRGRYDEEFAKILNKEALLFIADLQRTFRNHI 60

Query: 61  RYSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMII 120
           +YSMECRREAKRRYNEG LPGFDPATKYIR+SEWTCA VPPAVADRRVEITGPVERKMII
Sbjct: 61  KYSMECRREAKRRYNEGGLPGFDPATKYIRDSEWTCAPVPPAVADRRVEITGPVERKMII 120

Query: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKL 180
           NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHD+ RNRVYKLND+TAKL
Sbjct: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDRVRNRVYKLNDRTAKL 180

Query: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSR 240
           FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQG GPFFYLPKMEHSR
Sbjct: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGYGPFFYLPKMEHSR 240

Query: 241 EAKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYI 300
           EAKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYI
Sbjct: 241 EAKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYI 300

Query: 301 FSYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360
           FSYVKTFQAHPDRLLPDR+LVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP
Sbjct: 301 FSYVKTFQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360

Query: 361 KANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAA 420
           KANE ALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRS RR+DAA
Sbjct: 361 KANEVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSMRRDDAA 420

Query: 421 NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQN 480
           NLTEEDLLQ+PRGVRTMEGLRLNTRVGIQYLAAWLTG GSVPLYNL EDAATAEISRVQN
Sbjct: 421 NLTEEDLLQQPRGVRTMEGLRLNTRVGIQYLAAWLTGAGSVPLYNLAEDAATAEISRVQN 480

Query: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQC 540
           WQWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKE+F++GM+KEA KMFTRQC
Sbjct: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKMFTRQC 540

Query: 541 TAPSLDDFLTLDAYNRIVLHHPRELSRL 566
           TAP+LDDFLTLDAYN IV+HHPRELS+L
Sbjct: 541 TAPNLDDFLTLDAYNYIVIHHPRELSKL 568

[Close]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match: P24571 (Malate synthase, glyoxysomal OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 528/567 (93.12%), Postives = 547/567 (96.47%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+ILNKEALLFVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILNKEALLFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWH  EAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHFAEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAH DRLLPDR+ VGM QHFMRSYSDLLIRTCH    H +GGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHLDRLLPDRVQVGMAQHFMRSYSDLLIRTCHTVVCH-VGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFTN+MGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTNSMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LTE+DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTEDDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELF RVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 566

[Close]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match: P45458 (Malate synthase, glyoxysomal (Fragment) OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 2.1e-294
Identity = 481/564 (85.28%), Postives = 521/564 (92.38%), Query Frame = 0 

Query: 5   GMYSEPT---RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYSM 64
           G Y  PT   +K    YDVPEGVDIRGRYD EFAKIL K+AL FVADLQR FRNHI+Y++
Sbjct: 1   GTYGYPTPAVKKIESYYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNHIKYAL 60

Query: 65  ECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINALN 124
           ECRREAK++YNEGALP FDPAT YIRE EW CA VPPAVADR+VEITGPV+RKM+INALN
Sbjct: 61  ECRREAKKKYNEGALPEFDPATTYIREQEWVCAPVPPAVADRKVEITGPVDRKMVINALN 120

Query: 125 SGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVRP 184
           SGAKVFMADFEDALSP WENLMRGQ+NLKDAV GTIS HDKARNRVYKLNDQTAKLFVRP
Sbjct: 121 SGAKVFMADFEDALSPGWENLMRGQVNLKDAVAGTISLHDKARNRVYKLNDQTAKLFVRP 180

Query: 185 RGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAKI 244
           RGWHLPEAHI IDGEPATGCLVDFGLYFYH+++ FRR+QG G GPFFYLPKMEHSREAKI
Sbjct: 181 RGWHLPEAHILIDGEPATGCLVDFGLYFYHSYSAFRRTQGAGFGPFFYLPKMEHSREAKI 240

Query: 245 WNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYV 304
           WN+VFE+AE +AGIERGSIRATVLIETLPAVFQM+EILYEL+DHSVGLNCGRWDYIFSYV
Sbjct: 241 WNNVFEKAEKVAGIERGSIRATVLIETLPAVFQMNEILYELKDHSVGLNCGRWDYIFSYV 300

Query: 305 KTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANE 364
           KTFQAHPDRLLPDR+LVGMTQHFM+SYSDLLIRTCHRRGVHAMGGMAAQIPI++DP ANE
Sbjct: 301 KTFQAHPDRLLPDRVLVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIKEDPVANE 360

Query: 365 AALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLTE 424
            ALELVRKDKLREVKAGHDGTWAAHPGLIPACME+F NNMGNA NQI + +RED AN+TE
Sbjct: 361 VALELVRKDKLREVKAGHDGTWAAHPGLIPACMEIFNNNMGNASNQIDTVKREDGANITE 420

Query: 425 EDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWL 484
           +DLLQ PRG RTMEGLRLNTRVGIQY+AAWLTG+GSVPLYNLMEDAATAEISRVQNWQWL
Sbjct: 421 QDLLQIPRGARTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWL 480

Query: 485 KYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAPS 544
           KYGVEL+GDGLGV+VNKELFGRVVEEEM RIE+EVG EKF+ GM+KEA K+FTRQCT+P 
Sbjct: 481 KYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKEGMYKEACKIFTRQCTSPM 540

Query: 545 LDDFLTLDAYNRIVLHHPRELSRL 566
           LDDFLTLDAYN IV+HHPRE S+L
Sbjct: 541 LDDFLTLDAYNYIVVHHPRETSKL 564

[Close]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match: P17815 (Malate synthase, glyoxysomal OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 2.8e-294
Identity = 483/559 (86.40%), Postives = 521/559 (93.20%), Query Frame = 0 

Query: 8   SEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYSMECRREA 67
           S P +KT  GYDVPEGVDIRGRYD EFAKIL ++AL FVADLQR FRN IRY++ECR+EA
Sbjct: 9   SAPIKKTGAGYDVPEGVDIRGRYDGEFAKILTRDALQFVADLQREFRNRIRYAIECRKEA 68

Query: 68  KRRYNEGALPGFD-PATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINALNSGAKV 127
           K RYN GALPGF+ PATKYIRE EWTCA VPPAVADR+VEITGPVERKMIINALNSGAKV
Sbjct: 69  KSRYNAGALPGFEHPATKYIREGEWTCAPVPPAVADRKVEITGPVERKMIINALNSGAKV 128

Query: 128 FMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVRPRGWHL 187
           FMADFEDALSP+WENLMRGQ+NL+DAV+GTISFHDKARNRVYKLNDQ AKLFVRPRGWHL
Sbjct: 129 FMADFEDALSPSWENLMRGQVNLRDAVNGTISFHDKARNRVYKLNDQIAKLFVRPRGWHL 188

Query: 188 PEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAKIWNSVF 247
           PEAHI IDGEPATGCLVDFGLYFYHN+A FRR+QG G GPFFYLPKMEHSREAKIWN VF
Sbjct: 189 PEAHILIDGEPATGCLVDFGLYFYHNYAAFRRNQGAGYGPFFYLPKMEHSREAKIWNCVF 248

Query: 248 ERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYVKTFQA 307
           E+AE +AGIERGSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYIFSYVKTFQA
Sbjct: 249 EKAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQA 308

Query: 308 HPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANEAALEL 367
           HPDR LPDR+ VGMTQHFM+SYSDLL+ TCHRRGVHAMGGMAAQIPIRDDP AN+AALEL
Sbjct: 309 HPDRPLPDRVQVGMTQHFMKSYSDLLVWTCHRRGVHAMGGMAAQIPIRDDPAANKAALEL 368

Query: 368 VRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLTEEDLLQ 427
           VRKDKLREVKAGHDGTWAAHPGLIPACMEVF NNMGN P+QI++ +REDAAN+TEEDL+Q
Sbjct: 369 VRKDKLREVKAGHDGTWAAHPGLIPACMEVFANNMGNTPHQIQAMKREDAANITEEDLIQ 428

Query: 428 RPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVE 487
           RPRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVE
Sbjct: 429 RPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVE 488

Query: 488 LDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAPSLDDFL 547
           LDGDGLGV+V  +L GRVVE+EM RIEREVGKEKF++GM+KEA KMF RQC AP+LDDFL
Sbjct: 489 LDGDGLGVKVTFDLLGRVVEDEMARIEREVGKEKFKKGMYKEACKMFVRQCAAPTLDDFL 548

Query: 548 TLDAYNRIVLHHPRELSRL 566
           TLDAYN IV+H+P+  SRL
Sbjct: 549 TLDAYNNIVIHYPKGSSRL 567

[Close]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match: P17432 (Malate synthase, glyoxysomal OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 1.1e-293
Identity = 484/563 (85.97%), Postives = 523/563 (92.90%), Query Frame = 0 

Query: 1   MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS 60
           +GS G Y+ P+ K    YDVP+GVDIRGR+DEEFAKIL K+AL FVADLQR FRNHI+Y+
Sbjct: 4   LGSYG-YTAPSSKKINAYDVPQGVDIRGRFDEEFAKILTKDALQFVADLQREFRNHIKYA 63

Query: 61  MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL 120
           MECR+EAKRRYNEGALPGFDPAT+YIRE +WTC   PPAVADRRVEITGPVERKMIINAL
Sbjct: 64  MECRKEAKRRYNEGALPGFDPATRYIREGKWTCVPFPPAVADRRVEITGPVERKMIINAL 123

Query: 121 NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR 180
           NSGAKVFMADFEDALSP+WENLMRGQINLKDAV+GTI+F+DKARNRVYKLN++ AKLFVR
Sbjct: 124 NSGAKVFMADFEDALSPSWENLMRGQINLKDAVEGTITFNDKARNRVYKLNNEIAKLFVR 183

Query: 181 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK 240
           PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHN+A FR +QGQG GPFFYLPKME+SREAK
Sbjct: 184 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNYATFRNTQGQGFGPFFYLPKMENSREAK 243

Query: 241 IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY 300
           IWNSVFE+AE +AGIE+GSIRATVLIETLPAVFQMDEI YELRDHSVGLNCGRWDYIFSY
Sbjct: 244 IWNSVFEKAEKMAGIEKGSIRATVLIETLPAVFQMDEIFYELRDHSVGLNCGRWDYIFSY 303

Query: 301 VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN 360
           VKTFQ HPDRLLPDR  VGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP AN
Sbjct: 304 VKTFQGHPDRLLPDRGQVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPTAN 363

Query: 361 EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT 420
           EAA ELVRKDK REVKAGHDGTWAAHPGLI  CMEVFTNNMGN PNQI + +R+ A+NLT
Sbjct: 364 EAAFELVRKDKQREVKAGHDGTWAAHPGLIKTCMEVFTNNMGNTPNQIETVKRDYASNLT 423

Query: 421 EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW 480
           E+DLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTG+GSVPLYNLMEDAATAEISRVQ WQW
Sbjct: 424 EDDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQIWQW 483

Query: 481 LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP 540
           LKYGVELDGDGLGVRVN  +FGRVVEEEM RIEREVGKEKF++GM+KEA K+FTRQCTA 
Sbjct: 484 LKYGVELDGDGLGVRVN-HVFGRVVEEEMARIEREVGKEKFKKGMYKEACKIFTRQCTAS 543

Query: 541 SLDDFLTLDAYNRIVLHHPRELS 564
           +LDDFLTLDAYN IV+HHP+++S
Sbjct: 544 TLDDFLTLDAYNYIVIHHPKDVS 564

[Close]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match: A0A6J1CAL4 (Malate synthase OS=Momordica charantia OX=3673 GN=LOC111008963 PE=3 SV=1)

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0 

Query: 1   MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS 60
           MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS
Sbjct: 1   MGSLGMYSEPTRKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYS 60

Query: 61  MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL 120
           MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL
Sbjct: 61  MECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINAL 120

Query: 121 NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR 180
           NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR
Sbjct: 121 NSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVR 180

Query: 181 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK 240
           PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK
Sbjct: 181 PRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAK 240

Query: 241 IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY 300
           IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY
Sbjct: 241 IWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSY 300

Query: 301 VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN 360
           VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN
Sbjct: 301 VKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKAN 360

Query: 361 EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT 420
           EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT
Sbjct: 361 EAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLT 420

Query: 421 EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW 480
           EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW
Sbjct: 421 EEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQW 480

Query: 481 LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP 540
           LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP
Sbjct: 481 LKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAP 540

Query: 541 SLDDFLTLDAYNRIVLHHPRELSRL 566
           SLDDFLTLDAYNRIVLHHPRELSRL
Sbjct: 541 SLDDFLTLDAYNRIVLHHPRELSRL 565

[Close]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match: E3UZU8 (Malate synthase OS=Cucurbita maxima OX=3661 GN=LOC111495138 PE=2 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 536/567 (94.53%), Postives = 554/567 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+ILNKEALLFVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILNKEALLFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAH DRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHLDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFTN+MGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTNSMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LTE+DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTEDDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGVRVNKELF RVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 567

[Close]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match: A0A6J1E7A5 (Malate synthase OS=Cucurbita moschata OX=3662 GN=LOC111431278 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 553/567 (97.53%), Query Frame = 0 

Query: 1   MGSLGMYSEPT--RKTSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIR 60
           MGSLGMYSE    +K+SRGYDVPEGVDIRGRYDEEFA+IL+KEALLFVADLQRTFRNHIR
Sbjct: 1   MGSLGMYSESAVRKKSSRGYDVPEGVDIRGRYDEEFARILSKEALLFVADLQRTFRNHIR 60

Query: 61  YSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIIN 120
           YSMECRREAKRRYNEGA+PGFDPATKYIRESEWTCA VPPAVADRRVEITGPVERKMIIN
Sbjct: 61  YSMECRREAKRRYNEGAVPGFDPATKYIRESEWTCAPVPPAVADRRVEITGPVERKMIIN 120

Query: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLF 180
           ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARN+VYKLNDQTAKLF
Sbjct: 121 ALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNKVYKLNDQTAKLF 180

Query: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSRE 240
           VRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQGSGPFFYLPKMEHSRE
Sbjct: 181 VRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGSGPFFYLPKMEHSRE 240

Query: 241 AKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300
           AKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF
Sbjct: 241 AKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIF 300

Query: 301 SYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360
           SYVKTFQAHPDRLLPDR+ VGM QHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK
Sbjct: 301 SYVKTFQAHPDRLLPDRVQVGMAQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPK 360

Query: 361 ANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAAN 420
           ANE ALELVRKDKLRE KAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARR+DAAN
Sbjct: 361 ANEMALELVRKDKLREAKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARRDDAAN 420

Query: 421 LTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480
           LT +DLLQ+PRGVRT+EGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW
Sbjct: 421 LTADDLLQQPRGVRTLEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNW 480

Query: 481 QWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCT 540
           QWLKYGVELDGDGLGV VNKELFGRVVEEEM+RIEREVGKEKFR+GM+KEA KMFTRQCT
Sbjct: 481 QWLKYGVELDGDGLGVSVNKELFGRVVEEEMERIEREVGKEKFRKGMYKEACKMFTRQCT 540

Query: 541 APSLDDFLTLDAYNRIVLHHPRELSRL 566
           AP+LDDFLTLDAYN IV+HHPRELSRL
Sbjct: 541 APTLDDFLTLDAYNHIVIHHPRELSRL 567

[Close]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match: A0A0A0LUC5 (Malate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G050360 PE=3 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 536/568 (94.37%), Postives = 554/568 (97.54%), Query Frame = 0 

Query: 1   MGSLGMYSEP--TRK-TSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI 60
           MGSLGMYSE   T+K +SRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI
Sbjct: 1   MGSLGMYSESGLTKKGSSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI 60

Query: 61  RYSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMII 120
           +YSMECRREAKRRYNEG LPGFDPATKYIR+SEWTCA VPPAVADRRVEITGPVERKMII
Sbjct: 61  KYSMECRREAKRRYNEGGLPGFDPATKYIRDSEWTCAPVPPAVADRRVEITGPVERKMII 120

Query: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKL 180
           NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHD+ RNRVYKLNDQTAKL
Sbjct: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDRVRNRVYKLNDQTAKL 180

Query: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSR 240
           FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQG GPFFYLPKMEHSR
Sbjct: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGYGPFFYLPKMEHSR 240

Query: 241 EAKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYI 300
           EAKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYI
Sbjct: 241 EAKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYI 300

Query: 301 FSYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360
           FSYVKTFQAHPDRLLPDR+LVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP
Sbjct: 301 FSYVKTFQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360

Query: 361 KANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAA 420
           KANE ALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRS RR+DAA
Sbjct: 361 KANEVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSMRRDDAA 420

Query: 421 NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQN 480
           NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTG GSVPLYNLMEDAATAEISRVQN
Sbjct: 421 NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGAGSVPLYNLMEDAATAEISRVQN 480

Query: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQC 540
           WQWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKE+F++GM+KEA KMFTRQC
Sbjct: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKMFTRQC 540

Query: 541 TAPSLDDFLTLDAYNRIVLHHPRELSRL 566
           TAP+LDDFLTLDAYN IV+HHPRELS+L
Sbjct: 541 TAPNLDDFLTLDAYNYIVIHHPRELSKL 568

[Close]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match: A0A5D3BJH4 (Malate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004570 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 534/568 (94.01%), Postives = 555/568 (97.71%), Query Frame = 0 

Query: 1   MGSLGMYSEP--TRK-TSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI 60
           MGSLG+YSE   T+K +SRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI
Sbjct: 1   MGSLGIYSESGLTKKGSSRGYDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHI 60

Query: 61  RYSMECRREAKRRYNEGALPGFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMII 120
           +YSMECRREAKRRYNEG LPGFDPATKYIR+SEWTCA VPPAVADRRVEITGPVERKMII
Sbjct: 61  KYSMECRREAKRRYNEGGLPGFDPATKYIRDSEWTCAPVPPAVADRRVEITGPVERKMII 120

Query: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKL 180
           NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHD+ARNRVYKLNDQTAKL
Sbjct: 121 NALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFHDRARNRVYKLNDQTAKL 180

Query: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSR 240
           FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYF+HNHANFRRSQGQG GPFFYLPKMEHSR
Sbjct: 181 FVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRSQGQGYGPFFYLPKMEHSR 240

Query: 241 EAKIWNSVFERAENIAGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYI 300
           EAKIWNSVFERAE +AGIERGSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYI
Sbjct: 241 EAKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYI 300

Query: 301 FSYVKTFQAHPDRLLPDRILVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360
           FSYVKTFQAHPDRLLPDR+LVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP
Sbjct: 301 FSYVKTFQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDP 360

Query: 361 KANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAA 420
           KANE ALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVF+NNMGNAPNQI+S RR+DAA
Sbjct: 361 KANEVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEVFSNNMGNAPNQIQSMRRDDAA 420

Query: 421 NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQN 480
           NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTG GSVPLYNLMEDAATAEISRVQN
Sbjct: 421 NLTEEDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGAGSVPLYNLMEDAATAEISRVQN 480

Query: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQC 540
           WQWLKYGVELDGDGLGVRVNKELFGRVVEEEM+RIEREVGKE+F++GM+KEA KMFTRQC
Sbjct: 481 WQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKMFTRQC 540

Query: 541 TAPSLDDFLTLDAYNRIVLHHPRELSRL 566
           TAP+LDDFLTLDAYN IV+HHPRELS+L
Sbjct: 541 TAPNLDDFLTLDAYNYIVIHHPRELSKL 568

[Close]
BLAST of Moc03g29750 vs. TAIR 10
Match: AT5G03860.1 (malate synthase )

HSP 1 Score: 996.9 bits (2576), Expect = 6.6e-291
Identity = 467/548 (85.22%), Postives = 516/548 (94.16%), Query Frame = 0 

Query: 18  YDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYSMECRREAKRRYNEGALP 77
           YD P+GV++RGRYD+ FAKIL +EAL FVA+LQR FR H++Y+MECRREA+RRYN GA+P
Sbjct: 15  YDSPDGVEVRGRYDQIFAKILTREALSFVAELQREFRGHVKYAMECRREARRRYNSGAVP 74

Query: 78  GFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP 137
           GFDP+TK+IR+ +W+CASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP
Sbjct: 75  GFDPSTKFIRDGDWSCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP 134

Query: 138 NWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVRPRGWHLPEAHIFIDGEP 197
           +WENLMRG +NLKDAVDG+I+FHDK+RNRVYKLNDQTAKLFVRPRGWHLPEAHI IDGEP
Sbjct: 135 SWENLMRGHVNLKDAVDGSITFHDKSRNRVYKLNDQTAKLFVRPRGWHLPEAHILIDGEP 194

Query: 198 ATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAKIWNSVFERAENIAGIER 257
           ATGCLVDFGLYF+HN+A FR++QG G GPFFYLPKMEHSREAKIWNSVFERAE +AGIER
Sbjct: 195 ATGCLVDFGLYFFHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAEKMAGIER 254

Query: 258 GSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRIL 317
           GSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDR+L
Sbjct: 255 GSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRVL 314

Query: 318 VGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANEAALELVRKDKLREVKA 377
           VGM QHFMRSYSDLLIRTCH+RGVHAMGGMAAQIPIRDDPKANE AL+LVRKDKLREV+A
Sbjct: 315 VGMGQHFMRSYSDLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVRKDKLREVRA 374

Query: 378 GHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLTEEDLLQRPRGVRTMEGL 437
           GHDGTWAAHPGLIP CME FT +MG +PNQI+S +REDAA +TEEDLLQ PRGVRT+EGL
Sbjct: 375 GHDGTWAAHPGLIPICMEAFTGHMGKSPNQIKSVKREDAAAITEEDLLQIPRGVRTLEGL 434

Query: 438 RLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVELDGDGLGVRVN 497
           RLNTRVGIQYLAAWLTG+GSVPLYNLMEDAATAEISRVQNWQW++YGVELDGDGLGVRV+
Sbjct: 435 RLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGDGLGVRVS 494

Query: 498 KELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAPSLDDFLTLDAYNRIVLH 557
           KELFGRVVEEEM+RIE+EVGK+KF+ GM+KEA KMFT+QCTAP LDDFLTL  YN IV H
Sbjct: 495 KELFGRVVEEEMERIEKEVGKDKFKNGMYKEACKMFTKQCTAPELDDFLTLAVYNHIVAH 554

Query: 558 HPRELSRL 566
           +P  +SRL
Sbjct: 555 YPINVSRL 562

[Close]
BLAST of Moc03g29750 vs. TAIR 10
Match: AT5G03860.2 (malate synthase )

HSP 1 Score: 996.9 bits (2576), Expect = 6.6e-291
Identity = 467/548 (85.22%), Postives = 516/548 (94.16%), Query Frame = 0 

Query: 18  YDVPEGVDIRGRYDEEFAKILNKEALLFVADLQRTFRNHIRYSMECRREAKRRYNEGALP 77
           YD P+GV++RGRYD+ FAKIL +EAL FVA+LQR FR H++Y+MECRREA+RRYN GA+P
Sbjct: 15  YDSPDGVEVRGRYDQIFAKILTREALSFVAELQREFRGHVKYAMECRREARRRYNSGAVP 74

Query: 78  GFDPATKYIRESEWTCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP 137
           GFDP+TK+IR+ +W+CASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP
Sbjct: 75  GFDPSTKFIRDGDWSCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSP 134

Query: 138 NWENLMRGQINLKDAVDGTISFHDKARNRVYKLNDQTAKLFVRPRGWHLPEAHIFIDGEP 197
           +WENLMRG +NLKDAVDG+I+FHDK+RNRVYKLNDQTAKLFVRPRGWHLPEAHI IDGEP
Sbjct: 135 SWENLMRGHVNLKDAVDGSITFHDKSRNRVYKLNDQTAKLFVRPRGWHLPEAHILIDGEP 194

Query: 198 ATGCLVDFGLYFYHNHANFRRSQGQGSGPFFYLPKMEHSREAKIWNSVFERAENIAGIER 257
           ATGCLVDFGLYF+HN+A FR++QG G GPFFYLPKMEHSREAKIWNSVFERAE +AGIER
Sbjct: 195 ATGCLVDFGLYFFHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAEKMAGIER 254

Query: 258 GSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRIL 317
           GSIRATVLIETLPAVFQM+EILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDR+L
Sbjct: 255 GSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRVL 314

Query: 318 VGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANEAALELVRKDKLREVKA 377
           VGM QHFMRSYSDLLIRTCH+RGVHAMGGMAAQIPIRDDPKANE AL+LVRKDKLREV+A
Sbjct: 315 VGMGQHFMRSYSDLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVRKDKLREVRA 374

Query: 378 GHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSARREDAANLTEEDLLQRPRGVRTMEGL 437
           GHDGTWAAHPGLIP CME FT +MG +PNQI+S +REDAA +TEEDLLQ PRGVRT+EGL
Sbjct: 375 GHDGTWAAHPGLIPICMEAFTGHMGKSPNQIKSVKREDAAAITEEDLLQIPRGVRTLEGL 434

Query: 438 RLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVELDGDGLGVRVN 497
           RLNTRVGIQYLAAWLTG+GSVPLYNLMEDAATAEISRVQNWQW++YGVELDGDGLGVRV+
Sbjct: 435 RLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGDGLGVRVS 494

Query: 498 KELFGRVVEEEMDRIEREVGKEKFRRGMFKEAGKMFTRQCTAPSLDDFLTLDAYNRIVLH 557
           KELFGRVVEEEM+RIE+EVGK+KF+ GM+KEA KMFT+QCTAP LDDFLTL  YN IV H
Sbjct: 495 KELFGRVVEEEMERIEKEVGKDKFKNGMYKEACKMFTKQCTAPELDDFLTLAVYNHIVAH 554

Query: 558 HPRELSRL 566
           +P  +SRL
Sbjct: 555 YPINVSRL 562

The following BLAST results are available for this feature:
BLAST of Moc03g29750 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022137538.10.0e+00100.00malate synthase, glyoxysomal [Momordica charantia][more]
XP_023519701.10.0e+0094.53malate synthase, glyoxysomal [Cucurbita pepo subsp. pepo] >XP_023519702.1 malate... [more]
XP_023000792.10.0e+0094.53malate synthase, glyoxysomal [Cucurbita maxima] >XP_023000793.1 malate synthase,... [more]
KAG7019729.10.0e+0094.36hypothetical protein SDJN02_18692, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6584139.10.0e+0094.36hypothetical protein SDJN03_20071, partial [Cucurbita argyrosperma subsp. sorori... [more]
BLAST of Moc03g29750 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P082160.0e+0093.66Malate synthase, glyoxysomal OS=Cucumis sativus OX=3659 PE=2 SV=2[more]
P245710.0e+0093.12Malate synthase, glyoxysomal OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
P454582.1e-29485.28Malate synthase, glyoxysomal (Fragment) OS=Glycine max OX=3847 PE=2 SV=1[more]
P178152.8e-29486.40Malate synthase, glyoxysomal OS=Ricinus communis OX=3988 PE=2 SV=1[more]
P174321.1e-29385.97Malate synthase, glyoxysomal OS=Gossypium hirsutum OX=3635 PE=2 SV=1[more]
BLAST of Moc03g29750 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CAL40.0e+00100.00Malate synthase OS=Momordica charantia OX=3673 GN=LOC111008963 PE=3 SV=1[more]
E3UZU80.0e+0094.53Malate synthase OS=Cucurbita maxima OX=3661 GN=LOC111495138 PE=2 SV=1[more]
A0A6J1E7A50.0e+0094.36Malate synthase OS=Cucurbita moschata OX=3662 GN=LOC111431278 PE=3 SV=1[more]
A0A0A0LUC50.0e+0094.37Malate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G050360 PE=3 SV=1[more]
A0A5D3BJH40.0e+0094.01Malate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0045... [more]
BLAST of Moc03g29750 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G03860.16.6e-29185.22malate synthase [more]
AT5G03860.26.6e-29185.22malate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001465Malate synthasePFAMPF01274Malate_synthasecoord: 25..555
e-value: 3.0E-222
score: 738.5
IPR006252Malate synthase APIRSFPIRSF001363Malate_synthcoord: 15..556
e-value: 7.5E-243
score: 804.4
IPR006252Malate synthase ATIGRFAMTIGR01344TIGR01344coord: 37..554
e-value: 3.7E-221
score: 732.8
IPR006252Malate synthase APANTHERPTHR42902MALATE SYNTHASEcoord: 14..565
IPR006252Malate synthase ACDDcd00727malate_synt_Acoord: 37..555
e-value: 0.0
score: 891.991
NoneNo IPR availableGENE3D3.20.20.360Malate synthase, domain 3coord: 20..430
e-value: 6.8E-186
score: 619.5
NoneNo IPR availablePANTHERPTHR42902:SF4MALATE SYNTHASEcoord: 14..565
IPR044856Malate synthase, C-terminal superfamilyGENE3D1.20.1220.12Malate synthase, domain IIIcoord: 431..555
e-value: 1.6E-42
score: 146.5
IPR019830Malate synthase, conserved sitePROSITEPS00510MALATE_SYNTHASEcoord: 283..298
IPR011076Malate synthase superfamilySUPERFAMILY51645Malate synthase Gcoord: 18..555

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g29750.1Moc03g29750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006097 glyoxylate cycle
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0009514 glyoxysome
molecular_function GO:0004474 malate synthase activity
molecular_function GO:0003824 catalytic activity