Moc02g02370 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g02370
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	DNA polymerase epsilon subunit 3
Location	chr2: 1804013 .. 1806223 (+)
RNA-Seq Expression	Moc02g02370
Synteny	Moc02g02370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGAAAGTAGCGGCGGAGATCGAAGAGCTTCCCAAGACCATTGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCCCGGAACCAAGACATTTCCGTCAACAAAGACTCGCTTCTTGCCTTCTGCGAGAGCGCTCGAATCTTCATTCACTACCTCTCCGCTACGTAAGCTCGGCAACCTGCCCTAATGCTTTCAATTCTGAATGAAATTCTATTGATTCTGATGGTAGAAACTAGGGTTCCTTGTTTCTCTGCTGTTTACCTAAAATACGAACGCGAAACCGATTAGAGCCATGTATTTCTGGTTGTTATGGCGCTAGGGTTAACGAAATTGAAGTTTTGTACTGGTTAGTTCTTGTATTCGTACTAACTCGTAGATCTCTAAGCAGGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATAAAGGCGGATGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTCAAAGCCTCCCTCAATGGTGCGTTAGCTTCCCACTGCTGTGTTTACACTTTTTTTCAAAAAAGTATTTCTTGATGGATCGTGGAATTGCACGCTTAATGGGGAATTCTGTTTTTGATATTTCTACTTGCTAAACAACTTTAAAGGCATGATCTAGTCGTTCTCTTCGATGAGTATTACAGTTGTGCTATAGTTTACAATGTTGTATCTTGGTGTAAATTTTCTCCTTTCCTTCATCACTGTAGCTAAACTTCCCTTCTTGGAAGTGCTTCATGTAATCCCACCTATGGGCTTATACATCAATGAAATTGTTTCTTTTATAAAAAAAAAAATAAAAAAAAAATATGACAGTTGCTGCCGAACAACAGTGCCTTGTATTGCTAGACTTTCAGACTTCAAATTTAGATCATAAGCTTGATAGAACAGTGTTAACGAATAATATATAAATCATGTGTTGGATATTAAACTTCGATCTAATGGATTCTTTGTAAAACTAGGTGTCTAAAGATGAATGACTCTGCCATCGCTACCCCCCACCCCATTTTTTGTCTATTTGTTTGATAGAAGTCTCCTAGTCATTAAGTAGAGATATTGGTGAAATTTTTTATCTGCGCAATTCTTTCTACAAGTAAAATTCATGTAACCATCGATTTGAATGAGATAGTTTAGAGCTGATATAACTCACCCAAAATATATTTTCTTAAAGAATTACATGATCAGATCGAATAAGCTCCTAAAAAAGAAATATTCAAGTGCCTTTGAATCTATGGGACTTATTATTCTAGTTCTGTTTAGTTGTATCTGCAAATGGTATTGAGGTGATGAGTTCTTAAAACTTTGCTATATATCCAATGAGTGAGAGTTAGGATTTATTTCCGATGTTGTCCAGTTTTATTAGTAGAAGTACATACTTAGATCACATCAAAAAGCAGGTAAAGTGAGTAAGAATTTGTATTTACTGGTTCATAACCAGGTTTTGTTTATTGAAATCGGCAATAGTGGTATCCTGCAATGTACCAGAGCTAGTTGTGATTCCACTTGTAGTTGGACATGTGACGGAGAGAAAGTATGCGATCTTTGCTTAATGCCTACGTATAAAGACTTCTGAATTCGTAATGCTGCATGTAAGTTTAGCGTGAAGACAGTTTTTCTTTCTTTTCTCTTGATTAGGTATTATGTTGTCATTTTTGCTTGATTGTTATTATGTCAGAAAGAACAATTGGTCATGCTACAGTATGTCTGTATTCTTAGTGGACATTATATGAGCACAAGCTATTATTCAGAATTGTAGTTATTTCTTTTAAAATCTTAACGATTATTGTGGTTAGTTTAATGGAAAATAAATGATGTATCATATTGTATCCAAAAAGAGTAGTTGTTTTTTCTCCACGGGCTATGATTTTCCATCTGTTGGTCGCTGAATTAATGTTCAAACTTATCCGGTATTTGAATTAAGATAAATCTAATTGAAGTATTTATGGATTATGCAACCATTGTTTGGGTAAATGTAGAATTCAGGCGCAAGAGTGCGGGAAAGAAGGCAGCTGCATCCAGGGAAAAAGAAGCAAAAAAGAAGAGGAAGATAGAAGAGCCTACAGTTGAAGATGAAAGCAAAGATGACGTTGGAAATGACGATAACGATAACGATCACGATGAAGATGAAGATGAAGTTGAAGATGAAGATGAAGAAGAGAATGGTGCCAGTGATGAATGA

mRNA sequence

ATGGAGAAGAAAGTAGCGGCGGAGATCGAAGAGCTTCCCAAGACCATTGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCCCGGAACCAAGACATTTCCGTCAACAAAGACTCGCTTCTTGCCTTCTGCGAGAGCGCTCGAATCTTCATTCACTACCTCTCCGCTACGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATAAAGGCGGATGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTCAAAGCCTCCCTCAATGAATTCAGGCGCAAGAGTGCGGGAAAGAAGGCAGCTGCATCCAGGGAAAAAGAAGCAAAAAAGAAGAGGAAGATAGAAGAGCCTACAGTTGAAGATGAAAGCAAAGATGACGTTGGAAATGACGATAACGATAACGATCACGATGAAGATGAAGATGAAGTTGAAGATGAAGATGAAGAAGAGAATGGTGCCAGTGATGAATGA

Coding sequence (CDS)

ATGGAGAAGAAAGTAGCGGCGGAGATCGAAGAGCTTCCCAAGACCATTGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCCCGGAACCAAGACATTTCCGTCAACAAAGACTCGCTTCTTGCCTTCTGCGAGAGCGCTCGAATCTTCATTCACTACCTCTCCGCTACGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATAAAGGCGGATGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTCAAAGCCTCCCTCAATGAATTCAGGCGCAAGAGTGCGGGAAAGAAGGCAGCTGCATCCAGGGAAAAAGAAGCAAAAAAGAAGAGGAAGATAGAAGAGCCTACAGTTGAAGATGAAAGCAAAGATGACGTTGGAAATGACGATAACGATAACGATCACGATGAAGATGAAGATGAAGTTGAAGATGAAGATGAAGAAGAGAATGGTGCCAGTGATGAATGA

Protein sequence

MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE

Homology

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BLAST of Moc02g02370 vs. NCBI nr
Match: XP_022152961.1 (DNA polymerase epsilon subunit 3 [Momordica charantia])

HSP 1 Score: 284.3 bits (726), Expect = 6.8e-73
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
           KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE
Sbjct: 121 KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 164

[Close]

BLAST of Moc02g02370 vs. NCBI nr
Match: KAG6605978.1 (DNA polymerase epsilon subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.1 bits (601), Expect = 2.1e-58
Identity = 147/173 (84.97%), Postives = 154/173 (89.02%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDN------DHDEDEDEVEDEDEEENGASDE 165
             EEPTVE    ESKDDV ND++DN      D DEDEDEVEDEDE ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDKDEDEDEVEDEDEVENGGSDD 173

[Close]

BLAST of Moc02g02370 vs. NCBI nr
Match: KAG7035929.1 (DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.3 bits (599), Expect = 3.6e-58
Identity = 145/167 (86.83%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
             EEPTVE    ESKDDV ND++DN  D+DEDEVEDEDE ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDN--DKDEDEVEDEDEVENGGSDD 165

[Close]

BLAST of Moc02g02370 vs. NCBI nr
Match: XP_022995161.1 (DNA polymerase epsilon subunit 3 [Cucurbita maxima])

HSP 1 Score: 232.6 bits (592), Expect = 2.4e-57
Identity = 143/169 (84.62%), Postives = 154/169 (91.12%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESAR+FIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KIEEPTVE---DESKDDV--GNDDNDNDHDEDEDEVEDEDEEENGASDE 165
             EEPTVE    ESKDDV   NDD+D+D+D+DEDE ED+DE ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDKDEVENGGSDD 169

[Close]

BLAST of Moc02g02370 vs. NCBI nr
Match: XP_004140162.1 (DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymerase epsilon subunit D [Cucumis sativus] >KGN48033.1 hypothetical protein Csa_004222 [Cucumis sativus])

HSP 1 Score: 229.2 bits (583), Expect = 2.6e-56
Identity = 139/167 (83.23%), Postives = 148/167 (88.62%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKK+    EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASL+EFRRK+AGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
           K+EEPTVE    ES DDVGNDDND       D  EDEDE+EN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDND-------DNDEDEDEDENRASDD 160

[Close]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot
Match: Q3SZN5 (DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.7e-11
Identity = 52/147 (35.37%), Postives = 90/147 (61.22%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDES 131
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+   E   +D+S
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEE--QDKS 129

Query: 132 KDDVGNDDNDNDHDEDEDEVEDEDEEE 159
           +D+      DND DE+  E E+++EEE
Sbjct: 130 RDE------DNDEDEERLEEEEQNEEE 143

[Close]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot
Match: Q9NRF9 (DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.7e-11
Identity = 52/147 (35.37%), Postives = 90/147 (61.22%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDES 131
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+   E   +D+S
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEE--QDKS 129

Query: 132 KDDVGNDDNDNDHDEDEDEVEDEDEEE 159
           +D+      DND DE+  E E+++EEE
Sbjct: 130 RDE------DNDEDEERLEEEEQNEEE 143

[Close]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot
Match: Q5R4W3 (DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.7e-11
Identity = 52/147 (35.37%), Postives = 90/147 (61.22%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDES 131
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+   E   +D+S
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEE--QDKS 129

Query: 132 KDDVGNDDNDNDHDEDEDEVEDEDEEE 159
           +D+      DND DE+  E E+++EEE
Sbjct: 130 RDE------DNDEDEERLEEEEQNEEE 143

[Close]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot
Match: Q642A5 (DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-10
Identity = 49/152 (32.24%), Postives = 90/152 (59.21%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDES 131
            A DVL A+E+MEF   V PLK +L  +RR+  GKK A+ ++K+ K K+  EE   +D+S
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEE---QDKS 129

Query: 132 KDDVGNDDNDNDHDEDEDEVEDEDEEENGASD 164
           ++         + DEDE+ +++E++ E    D
Sbjct: 130 RE---------EEDEDEERLDEEEQNEEEEVD 144

[Close]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot
Match: Q9JKP7 (DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.5e-09
Identity = 47/144 (32.64%), Postives = 88/144 (61.11%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKRKIEEPTVEDES 131
            A DVL A+E+MEF   + PLK +L  +RR   GKK A+ ++K+ K K+  EE   +D+S
Sbjct: 70  NASDVLSAMEEMEFQRFITPLKEALEAYRRDEKGKKEASEQKKKDKDKKDSEE---QDKS 129

Query: 132 KDDVGNDDNDNDHDEDEDEVEDED 156
           + +V  +D +   ++D++E E+ D
Sbjct: 130 R-EVEEEDEERLDEDDQNEEEEID 144

[Close]

BLAST of Moc02g02370 vs. ExPASy TrEMBL
Match: A0A6J1DJA4 (DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.3e-73
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
           KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE
Sbjct: 121 KIEEPTVEDESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 164

[Close]

BLAST of Moc02g02370 vs. ExPASy TrEMBL
Match: A0A6J1K184 (DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.1e-57
Identity = 143/169 (84.62%), Postives = 154/169 (91.12%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESAR+FIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KIEEPTVE---DESKDDV--GNDDNDNDHDEDEDEVEDEDEEENGASDE 165
             EEPTVE    ESKDDV   NDD+D+D+D+DEDE ED+DE ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDKDEVENGGSDD 169

[Close]

BLAST of Moc02g02370 vs. ExPASy TrEMBL
Match: A0A0A0KEV7 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425700 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-56
Identity = 139/167 (83.23%), Postives = 148/167 (88.62%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKK+    EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASL+EFRRK+AGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
           K+EEPTVE    ES DDVGNDDND       D  EDEDE+EN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDND-------DNDEDEDEDENRASDD 160

[Close]

BLAST of Moc02g02370 vs. ExPASy TrEMBL
Match: A0A6J1H495 (DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.2e-56
Identity = 142/167 (85.03%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 165
             EEPTVE    ESKDDV ND++DND   D+D  EDEDE ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKD--EDEDEVENGGSDD 165

[Close]

BLAST of Moc02g02370 vs. ExPASy TrEMBL
Match: A0A1S3BNA4 (DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 6.3e-56
Identity = 131/151 (86.75%), Postives = 139/151 (92.05%), Query Frame = 0


Query: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKK+ A IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFRRK+AGKKAAA REKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDE 149
           K+EEPTVE    ES D VG+DDND D DE+E
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDNDEDEDENE 151

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BLAST of Moc02g02370 vs. TAIR 10
Match: AT2G27470.1 (nuclear factor Y, subunit B11 )

HSP 1 Score: 134.4 bits (337), Expect = 8.1e-32
Identity = 90/195 (46.15%), Postives = 129/195 (66.15%), Query Frame = 0


Query: 9   IEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKR 68
           ++ELP  IVRRVVK+KLS+CS + D+S++K++LLAF ESARIFIHYLSAT+ND CK+++R
Sbjct: 8   VDELPLAIVRRVVKKKLSECSPDYDVSIHKEALLAFSESARIFIHYLSATANDFCKDARR 67

Query: 69  QTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKA-------------------A 128
           QT+KADDV KALE+M+F E + PLK+SL +F++K+AGKKA                    
Sbjct: 68  QTMKADDVFKALEEMDFSEFLEPLKSSLEDFKKKNAGKKAGAAAASYPAGGAALKSSSGT 127

Query: 129 ASREKEAKKKRKIE--------------------EPTVEDESKDDVGNDDNDNDHDEDED 165
           AS+ KE KK+++ E                    E T  D ++++ GND+ D + +++ED
Sbjct: 128 ASKPKETKKRKQEEPSTQKGARKSKIDEETKRNDEETENDNTEEENGNDEEDENGNDEED 187

[Close]

BLAST of Moc02g02370 vs. TAIR 10
Match: AT5G23090.2 (nuclear factor Y, subunit B13 )

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-05
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKADDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  + VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Moc02g02370 vs. TAIR 10
Match: AT5G23090.1 (nuclear factor Y, subunit B13 )

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-05
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKADDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  + VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Moc02g02370 vs. TAIR 10
Match: AT5G23090.4 (nuclear factor Y, subunit B13 )

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-05
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKADDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  + VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Moc02g02370 vs. TAIR 10
Match: AT5G08190.1 (nuclear factor Y, subunit B12 )

HSP 1 Score: 46.2 bits (108), Expect = 2.9e-05
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SN++C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----ADVRVARDAQDLLIECCVEFINLISSESNEVCNKED 71

Query: 68  RQTIKADDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  + VLKAL+ + F E V  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

The following BLAST results are available for this feature:

BLAST of Moc02g02370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152961.1	6.8e-73	100.00	DNA polymerase epsilon subunit 3 [Momordica charantia]	[more]
KAG6605978.1	2.1e-58	84.97	DNA polymerase epsilon subunit 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
KAG7035929.1	3.6e-58	86.83	DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022995161.1	2.4e-57	84.62	DNA polymerase epsilon subunit 3 [Cucurbita maxima]	[more]
XP_004140162.1	2.6e-56	83.23	DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymeras...	[more]

BLAST of Moc02g02370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZN5	7.7e-11	35.37	DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1	[more]
Q9NRF9	7.7e-11	35.37	DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1	[more]
Q5R4W3	7.7e-11	35.37	DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1	[more]
Q642A5	6.5e-10	32.24	DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=...	[more]
Q9JKP7	5.5e-09	32.64	DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1	[more]

BLAST of Moc02g02370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJA4	3.3e-73	100.00	DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 ...	[more]
A0A6J1K184	1.1e-57	84.62	DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=...	[more]
A0A0A0KEV7	1.3e-56	83.23	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4257...	[more]
A0A6J1H495	2.2e-56	85.03	DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 P...	[more]
A0A1S3BNA4	6.3e-56	86.75	DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV...	[more]

BLAST of Moc02g02370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27470.1	8.1e-32	46.15	nuclear factor Y, subunit B11	[more]
AT5G23090.2	1.7e-05	30.11	nuclear factor Y, subunit B13	[more]
AT5G23090.1	1.7e-05	30.11	nuclear factor Y, subunit B13	[more]
AT5G23090.4	1.7e-05	30.11	nuclear factor Y, subunit B13	[more]
AT5G08190.1	2.9e-05	30.11	nuclear factor Y, subunit B12	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 11..80 e-value: 1.3E-8 score: 34.9
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 2..161 e-value: 2.2E-32 score: 113.7
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 12..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 129..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 100..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 100..164
None	No IPR available	PANTHER	PTHR46172	DNA POLYMERASE EPSILON SUBUNIT 3	coord: 2..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g02370.1	Moc02g02370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006974	cellular response to DNA damage stimulus
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0042766	nucleosome mobilization
cellular_component	GO:0008623	CHRAC
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0031490	chromatin DNA binding
molecular_function	GO:0046982	protein heterodimerization activity