Moc01g25840 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g25840
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING-type E3 ubiquitin transferase
Locationchr1: 18394228 .. 18395272 (+)
RNA-Seq ExpressionMoc01g25840
SyntenyMoc01g25840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGATAGCAAAAGCTCCAAGTCAAATATCATGCCAACAACCATCTGAAAGGTTTGTTCCTGAAATAGTTTCTTCTTCTGTACTGGAGATTAGATCTGATCTCACTGGCATCAAAGCCAAAGTTCGAAAGCTTGTTGAGGAATTGACGAGCGATTCACTTGAAACTCTAAGAACAACTACAGCTGAACTACGGTTGCTTACCAAGCTTAGTGTAAAAAATCGCAGGGTAATTGCAGATTCTGGGGCAATTAGCCTTTTAGTTAATTTGCTAAATTCTAGGGACACAAAGATTCAAGAGAATGCTGTTACAGCACTTGTGAACTTATCAATCGACAATGATTGCAAAACTATGATTGTTCAGGCCAATGCAATTGAACCTTTGGTTAAGATCCTTCAAACTGGCAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTGATTGATGATAACCAGGTTAACATCGGGAGGTCCGGGGCAATAGAAGCTCTGGTTGACTTGTTGAGGGATGGCACGCCTCAGGGAAAGAAAGATGCAGCCACTGCACTGTTTAATTTGTCATTGCTTACTGAAAACAAGGCCAAGATTGTGCAGGCTGGATCTATCAAGCATCTTGTGGAGCTGATGGATCCATCTGCTGGAATGGTTGAGAAGGCAGTTGTTGTTTTGGCAAATCTTGCATCAGTAGATGAAGGAAGGATTAAAATCGTACGTGAGGGTGGTATCAGTTTGTTGGTTGACACTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTATGGATTTGTGCAACCGCTAGTAGATTTTGCATTATGGCAATCCAAGAGGGAGCTATCCCTCCATTAGTGGTGCTGTCACAATCGGGCACAACGAGGGCCAAGGATAAGGTACATGACTGCAATGAATTTTGTTTTCCTATGATTCCAAATTCGCAATAGAATTTAAAGAGGAAATTTTAACTACTTGCAGGCTCGGGCATTACTCAGTTGCTTCAGCAGGAATAAACTCGTATGA

mRNA sequence

ATGTTGATAGCAAAAGCTCCAAGTCAAATATCATGCCAACAACCATCTGAAAGGTTTGTTCCTGAAATAGTTTCTTCTTCTGTACTGGAGATTAGATCTGATCTCACTGGCATCAAAGCCAAAGTTCGAAAGCTTGTTGAGGAATTGACGAGCGATTCACTTGAAACTCTAAGAACAACTACAGCTGAACTACGGTTGCTTACCAAGCTTAGTGTAAAAAATCGCAGGGTAATTGCAGATTCTGGGGCAATTAGCCTTTTAGTTAATTTGCTAAATTCTAGGGACACAAAGATTCAAGAGAATGCTGTTACAGCACTTGTGAACTTATCAATCGACAATGATTGCAAAACTATGATTGTTCAGGCCAATGCAATTGAACCTTTGGTTAAGATCCTTCAAACTGGCAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTGATTGATGATAACCAGGTTAACATCGGGAGGTCCGGGGCAATAGAAGCTCTGGTTGACTTGTTGAGGGATGGCACGCCTCAGGGAAAGAAAGATGCAGCCACTGCACTGTTTAATTTGTCATTGCTTACTGAAAACAAGGCCAAGATTGTGCAGGCTGGATCTATCAAGCATCTTGTGGAGCTGATGGATCCATCTGCTGGAATGGTTGAGAAGGCAGTTGTTGTTTTGGCAAATCTTGCATCAGTAGATGAAGGAAGGATTAAAATCGTACGTGAGGGTGGTATCAGTTTGTTGGTTGACACTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTATGGATTTGTGCAACCGCTAGTAGATTTTGCATTATGGCAATCCAAGAGGGAGCTATCCCTCCATTAGTGGTGCTGTCACAATCGGGCACAACGAGGGCCAAGGATAAGGCTCGGGCATTACTCAGTTGCTTCAGCAGGAATAAACTCGTATGA

Coding sequence (CDS)

ATGTTGATAGCAAAAGCTCCAAGTCAAATATCATGCCAACAACCATCTGAAAGGTTTGTTCCTGAAATAGTTTCTTCTTCTGTACTGGAGATTAGATCTGATCTCACTGGCATCAAAGCCAAAGTTCGAAAGCTTGTTGAGGAATTGACGAGCGATTCACTTGAAACTCTAAGAACAACTACAGCTGAACTACGGTTGCTTACCAAGCTTAGTGTAAAAAATCGCAGGGTAATTGCAGATTCTGGGGCAATTAGCCTTTTAGTTAATTTGCTAAATTCTAGGGACACAAAGATTCAAGAGAATGCTGTTACAGCACTTGTGAACTTATCAATCGACAATGATTGCAAAACTATGATTGTTCAGGCCAATGCAATTGAACCTTTGGTTAAGATCCTTCAAACTGGCAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTGATTGATGATAACCAGGTTAACATCGGGAGGTCCGGGGCAATAGAAGCTCTGGTTGACTTGTTGAGGGATGGCACGCCTCAGGGAAAGAAAGATGCAGCCACTGCACTGTTTAATTTGTCATTGCTTACTGAAAACAAGGCCAAGATTGTGCAGGCTGGATCTATCAAGCATCTTGTGGAGCTGATGGATCCATCTGCTGGAATGGTTGAGAAGGCAGTTGTTGTTTTGGCAAATCTTGCATCAGTAGATGAAGGAAGGATTAAAATCGTACGTGAGGGTGGTATCAGTTTGTTGGTTGACACTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTATGGATTTGTGCAACCGCTAGTAGATTTTGCATTATGGCAATCCAAGAGGGAGCTATCCCTCCATTAGTGGTGCTGTCACAATCGGGCACAACGAGGGCCAAGGATAAGGCTCGGGCATTACTCAGTTGCTTCAGCAGGAATAAACTCGTATGA

Protein sequence

MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNKLV
Homology
BLAST of Moc01g25840 vs. NCBI nr
Match: XP_022133100.1 (U-box domain-containing protein 4-like isoform X1 [Momordica charantia] >XP_022133101.1 U-box domain-containing protein 4-like isoform X1 [Momordica charantia] >XP_022133102.1 U-box domain-containing protein 4-like isoform X1 [Momordica charantia])

HSP 1 Score: 581.3 bits (1497), Expect = 5.3e-162
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT
Sbjct: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV
Sbjct: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG
Sbjct: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK
Sbjct: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT
Sbjct: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKLV 321
           TRAKDKARALLSCFSRNKLV
Sbjct: 301 TRAKDKARALLSCFSRNKLV 320

BLAST of Moc01g25840 vs. NCBI nr
Match: XP_008440337.1 (PREDICTED: U-box domain-containing protein 4 [Cucumis melo] >XP_008440338.1 PREDICTED: U-box domain-containing protein 4 [Cucumis melo])

HSP 1 Score: 489.6 bits (1259), Expect = 2.1e-134
Identity = 267/319 (83.70%), Postives = 292/319 (91.54%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQISCQQP ER V EIVS+ VLE+ SDLTGIKAKV+KLVE+L SDSLETLRT+
Sbjct: 1   MLIAK--SQISCQQPYERMVHEIVSTPVLEVISDLTGIKAKVQKLVEDLRSDSLETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   N  VIAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CKT+IV
Sbjct: 61  TAELRLLTKIDANNWTVIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKTIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRSGAI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSGAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI+LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIALLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKL 320
            RAK+KARALL+CFSRNKL
Sbjct: 301 ARAKEKARALLTCFSRNKL 317

BLAST of Moc01g25840 vs. NCBI nr
Match: XP_031742786.1 (U-box domain-containing protein 4 [Cucumis sativus] >KGN48575.1 hypothetical protein Csa_003656 [Cucumis sativus])

HSP 1 Score: 481.1 bits (1237), Expect = 7.4e-132
Identity = 265/319 (83.07%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQI CQQP ER V EIVSS VLE  SD TGIKAKV+KLVE LTSDS ETLRT+
Sbjct: 1   MLIAK--SQIPCQQPYERMVHEIVSSPVLEAISDHTGIKAKVQKLVENLTSDSPETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   NR +IAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CK++IV
Sbjct: 61  TAELRLLTKIDANNRTLIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKSIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRS AI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSRAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIPLLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKL 320
            RAK+KARALLSCFSRNKL
Sbjct: 301 ARAKEKARALLSCFSRNKL 317

BLAST of Moc01g25840 vs. NCBI nr
Match: TYK12846.1 (U-box domain-containing protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-127
Identity = 255/306 (83.33%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQISCQQP ER V EIVS+ VLE+ SDLTGIKAKV+KLVE+L SDSLETLRT+
Sbjct: 1   MLIAK--SQISCQQPYERMVHEIVSTPVLEVISDLTGIKAKVQKLVEDLRSDSLETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   N  VIAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CKT+IV
Sbjct: 61  TAELRLLTKIDANNWTVIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKTIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRSGAI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSGAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI+LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIALLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDK 307
            RAK+K
Sbjct: 301 ARAKEK 304

BLAST of Moc01g25840 vs. NCBI nr
Match: XP_038880844.1 (U-box domain-containing protein 4-like [Benincasa hispida] >XP_038880845.1 U-box domain-containing protein 4-like [Benincasa hispida] >XP_038880846.1 U-box domain-containing protein 4-like [Benincasa hispida] >XP_038880847.1 U-box domain-containing protein 4-like [Benincasa hispida])

HSP 1 Score: 466.1 bits (1198), Expect = 2.5e-127
Identity = 254/297 (85.52%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 23  IVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSG 82
           IVSS VLE RSD T IKAKV+KLVE+LTSDSLETLRT+TAE+RLLTK++  NRRVIAD G
Sbjct: 5   IVSSPVLEARSDHTDIKAKVQKLVEDLTSDSLETLRTSTAEMRLLTKINANNRRVIADYG 64

Query: 83  AISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKEN 142
           AI +LVNLLNS DTKIQENAVTALVNLSID +CKT+IVQANAIEPL+ +LQTGSPEAKEN
Sbjct: 65  AIKVLVNLLNSTDTKIQENAVTALVNLSIDYNCKTIIVQANAIEPLIHVLQTGSPEAKEN 124

Query: 143 SAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIV 202
           SAATLGSLSVIDDNQVNIGRS AI  LVDLLRDGTP+G +DAATALFNLSLL+ENKAKIV
Sbjct: 125 SAATLGSLSVIDDNQVNIGRSRAIGHLVDLLRDGTPRGMRDAATALFNLSLLSENKAKIV 184

Query: 203 QAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAK 262
           +AGSIKHLV+LMDP+AGMVEKAVVVLANLAS+DEGRI+IV EGGI+LLVDT+ELGSARAK
Sbjct: 185 EAGSIKHLVKLMDPAAGMVEKAVVVLANLASIDEGRIEIVCEGGIALLVDTIELGSARAK 244

Query: 263 EYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNKL 320
           EYAAAALLW+CAT SRFCIMAIQEGAIPPLV LS SGT RAK+KARALLSCFSRNKL
Sbjct: 245 EYAAAALLWLCATTSRFCIMAIQEGAIPPLVALSHSGTARAKEKARALLSCFSRNKL 301

BLAST of Moc01g25840 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-91
Identity = 191/306 (62.42%), Postives = 240/306 (78.43%), Query Frame = 0

Query: 13  QQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSV 72
           ++PSER    IVS+   E R DL+ ++ +V+KLVEEL S SL+T R  TAELRLL K ++
Sbjct: 514 RRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNM 573

Query: 73  KNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKIL 132
            NR VI +SGAI LLV LL S D+  QENAVTAL+NLSI+++ K  I  A AIEPL+ +L
Sbjct: 574 DNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVL 633

Query: 133 QTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLS 192
           + GS EAKENSAATL SLSVI++N++ IG+SGAI  LVDLL +GTP+GKKDAATALFNLS
Sbjct: 634 ENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLS 693

Query: 193 LLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVD 252
           +  ENKA IVQ+G++++L++LMDP+AGMV+KAV VLANLA++ EGR  I +EGGI LLV+
Sbjct: 694 IHQENKAMIVQSGAVRYLIDLMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVE 753

Query: 253 TVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLS 312
            VELGSAR KE AAAALL +   + RFC M +QEGA+PPLV LSQSGT RA++KA+ALLS
Sbjct: 754 VVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLS 813

Query: 313 CFSRNK 319
            F   +
Sbjct: 814 YFRNQR 819

BLAST of Moc01g25840 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 165/299 (55.18%), Postives = 221/299 (73.91%), Query Frame = 0

Query: 21  PEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIAD 80
           P I+ ++V E  S  + I+ +V+KL+++L S SL+T R  TA +R+L + S  NR VIA 
Sbjct: 403 PGIIPATVRETGSS-SSIETEVKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIAR 462

Query: 81  SGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKILQTG-SPEA 140
             AI  LV+LL S D +IQ +AVT L+NLSI+++ K++I ++ AI PL+ +L+TG   EA
Sbjct: 463 CEAIPSLVSLLYSTDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEA 522

Query: 141 KENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKA 200
           K NSAATL SLSVI++ +  IG +GAIE LVDLL  G+  GKKDAATALFNLS+  ENK 
Sbjct: 523 KANSAATLFSLSVIEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKT 582

Query: 201 KIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSA 260
           K+++AG++++LVELMDP+ GMVEKAVVVLANLA+V EG+I I  EGGI +LV+ VELGSA
Sbjct: 583 KVIEAGAVRYLVELMDPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSA 642

Query: 261 RAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNK 319
           R KE A AALL +C  + +FC   I+EG IPPLV L++SGT R K+KA+ LL  F  ++
Sbjct: 643 RGKENATAALLQLCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKAHR 700

BLAST of Moc01g25840 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-70
Identity = 160/276 (57.97%), Postives = 204/276 (73.91%), Query Frame = 0

Query: 44  KLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAV 103
           KLVE+L S S +      AE+R LT  S++NR  I   GAI+ L++LL S +   QE+AV
Sbjct: 476 KLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAV 535

Query: 104 TALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRS 163
           TAL+NLSI    K MIV+  AIEPLV +L TG+  AKENSAA+L SLSV+  N+  IG+S
Sbjct: 536 TALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQS 595

Query: 164 -GAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVE 223
             AI+ALV+LL  GT +GKKDAA+ALFNLS+  +NKA+IVQA ++K+LVEL+DP   MV+
Sbjct: 596 NAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELLDPDLEMVD 655

Query: 224 KAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIM 283
           KAV +LANL++V EGR  IVREGGI LLV+TV+LGS R KE AA+ LL +C  + +FC +
Sbjct: 656 KAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTL 715

Query: 284 AIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNK 319
            +QEGAIPPLV LSQSGT RAK+KA+ LLS F   +
Sbjct: 716 VLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 751

BLAST of Moc01g25840 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.5e-54
Identity = 128/268 (47.76%), Postives = 183/268 (68.28%), Query Frame = 0

Query: 45  LVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVT 104
           L+  L S + +  R    E+RLL K +V NR  IA++GAI LLVNLL+S D + QE+AVT
Sbjct: 328 LMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVT 387

Query: 105 ALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSG 164
           AL+NLSI  + K  IV ++AI  +V++L+TGS E +EN+AATL SLSV+D+N+V IG +G
Sbjct: 388 ALLNLSIHENNKASIVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAG 447

Query: 165 AIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVE-LMDPSAGMVEK 224
           AI  L++LL DG+P+GKKDAATA+FNL +   NK + V+AG + HL+  L+DP+ GM+++
Sbjct: 448 AIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDE 507

Query: 225 AVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMA 284
           A+ +L+ LA   EG+I I R   I  LV+ ++ GS R +E AAA L  +C+  +   + A
Sbjct: 508 ALSLLSILAGNPEGKIVIARSEPIPPLVEVIKTGSPRNRENAAAILWLLCSADTEQTLAA 567

Query: 285 IQEGAIPPLVVLSQSGTTRAKDKARALL 312
              G    L  LS++GT RAK KA ++L
Sbjct: 568 KAAGVEDALKELSETGTDRAKRKASSIL 595

BLAST of Moc01g25840 vs. ExPASy Swiss-Prot
Match: Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.8e-51
Identity = 131/287 (45.64%), Postives = 190/287 (66.20%), Query Frame = 0

Query: 34  DLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNS 93
           DL+G  + +R LV +L+S S+E  RT  +E+R L+K S  NR +IA++GAI +LV LL S
Sbjct: 335 DLSGDMSAIRALVCKLSSQSIEDRRTAVSEIRSLSKRSTDNRILIAEAGAIPVLVKLLTS 394

Query: 94  -RDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSV 153
             DT+ QENAVT ++NLSI    K +I+ A A+  +V +L+ GS EA+EN+AATL SLS+
Sbjct: 395 DGDTETQENAVTCILNLSIYEHNKELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSL 454

Query: 154 IDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVE 213
            D+N++ IG SGAI ALVDLL+ G+ +GKKDAATALFNL +   NK + V+AG +K LV+
Sbjct: 455 ADENKIIIGASGAIMALVDLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVK 514

Query: 214 LMDPSAG--MVEKAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALL 273
           ++  S+   M ++A+ +L+ LAS    +  I+R   I  L+D ++    R +E AAA LL
Sbjct: 515 MLTDSSSERMADEALTILSVLASNQVAKTAILRANAIPPLIDCLQKDQPRNRENAAAILL 574

Query: 274 WICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRN 318
            +C   +   I   + GA+ PL+ LS+ GT RAK KA +LL    ++
Sbjct: 575 CLCKRDTEKLISIGRLGAVVPLMELSRDGTERAKRKANSLLELLRKS 621

BLAST of Moc01g25840 vs. ExPASy TrEMBL
Match: A0A6J1BVN7 (U-box domain-containing protein 4-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005781 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT
Sbjct: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV
Sbjct: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG
Sbjct: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK
Sbjct: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT
Sbjct: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKLV 321
           TRAKDKARALLSCFSRNKLV
Sbjct: 301 TRAKDKARALLSCFSRNKLV 320

BLAST of Moc01g25840 vs. ExPASy TrEMBL
Match: A0A1S3B0V3 (U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103484819 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 267/319 (83.70%), Postives = 292/319 (91.54%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQISCQQP ER V EIVS+ VLE+ SDLTGIKAKV+KLVE+L SDSLETLRT+
Sbjct: 1   MLIAK--SQISCQQPYERMVHEIVSTPVLEVISDLTGIKAKVQKLVEDLRSDSLETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   N  VIAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CKT+IV
Sbjct: 61  TAELRLLTKIDANNWTVIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKTIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRSGAI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSGAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI+LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIALLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKL 320
            RAK+KARALL+CFSRNKL
Sbjct: 301 ARAKEKARALLTCFSRNKL 317

BLAST of Moc01g25840 vs. ExPASy TrEMBL
Match: A0A0A0KFV9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493830 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.6e-132
Identity = 265/319 (83.07%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQI CQQP ER V EIVSS VLE  SD TGIKAKV+KLVE LTSDS ETLRT+
Sbjct: 1   MLIAK--SQIPCQQPYERMVHEIVSSPVLEAISDHTGIKAKVQKLVENLTSDSPETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   NR +IAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CK++IV
Sbjct: 61  TAELRLLTKIDANNRTLIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKSIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRS AI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSRAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIPLLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDKARALLSCFSRNKL 320
            RAK+KARALLSCFSRNKL
Sbjct: 301 ARAKEKARALLSCFSRNKL 317

BLAST of Moc01g25840 vs. ExPASy TrEMBL
Match: A0A5D3CR20 (U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004380 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.0e-128
Identity = 255/306 (83.33%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAK  SQISCQQP ER V EIVS+ VLE+ SDLTGIKAKV+KLVE+L SDSLETLRT+
Sbjct: 1   MLIAK--SQISCQQPYERMVHEIVSTPVLEVISDLTGIKAKVQKLVEDLRSDSLETLRTS 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
           TAELRLLTK+   N  VIAD GAISLLVNLLNS DTKIQENAVTALVNLSIDN+CKT+IV
Sbjct: 61  TAELRLLTKIDANNWTVIADYGAISLLVNLLNSTDTKIQENAVTALVNLSIDNNCKTIIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPL+ +LQTGSPEAKENSAATLGSLSV+DDNQVNIGRSGAI  LVDLL+DGTP+G
Sbjct: 121 QANAIEPLIHVLQTGSPEAKENSAATLGSLSVVDDNQVNIGRSGAIGPLVDLLKDGTPRG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           K+DAATALFNLSLL+ENK KIV+AGSIKHLV+LMDP+ GMVEKAV VLANLAS DEGRI+
Sbjct: 181 KRDAATALFNLSLLSENKPKIVEAGSIKHLVKLMDPATGMVEKAVTVLANLASTDEGRIE 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGI+LLVDT+ELGSARAKEYAAAALLW+C   SR+CIMAIQEGAIPPLV LSQSGT
Sbjct: 241 IVREGGIALLVDTIELGSARAKEYAAAALLWLCGITSRYCIMAIQEGAIPPLVALSQSGT 300

Query: 301 TRAKDK 307
            RAK+K
Sbjct: 301 ARAKEK 304

BLAST of Moc01g25840 vs. ExPASy TrEMBL
Match: A0A6J1BY41 (U-box domain-containing protein 4-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005781 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 262/320 (81.88%), Postives = 262/320 (81.88%), Query Frame = 0

Query: 1   MLIAKAPSQISCQQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTT 60
           MLIAKAPSQISCQQPSE                                           
Sbjct: 1   MLIAKAPSQISCQQPSE------------------------------------------- 60

Query: 61  TAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120
                          RVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV
Sbjct: 61  ---------------RVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIV 120

Query: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180
           QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG
Sbjct: 121 QANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQG 180

Query: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240
           KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK
Sbjct: 181 KKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIK 240

Query: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 300
           IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT
Sbjct: 241 IVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGT 262

Query: 301 TRAKDKARALLSCFSRNKLV 321
           TRAKDKARALLSCFSRNKLV
Sbjct: 301 TRAKDKARALLSCFSRNKLV 262

BLAST of Moc01g25840 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 337.4 bits (864), Expect = 1.2e-92
Identity = 191/306 (62.42%), Postives = 240/306 (78.43%), Query Frame = 0

Query: 13  QQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSV 72
           ++PSER    IVS+   E R DL+ ++ +V+KLVEEL S SL+T R  TAELRLL K ++
Sbjct: 517 RRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNM 576

Query: 73  KNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKIL 132
            NR VI +SGAI LLV LL S D+  QENAVTAL+NLSI+++ K  I  A AIEPL+ +L
Sbjct: 577 DNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVL 636

Query: 133 QTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLS 192
           + GS EAKENSAATL SLSVI++N++ IG+SGAI  LVDLL +GTP+GKKDAATALFNLS
Sbjct: 637 ENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLS 696

Query: 193 LLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVD 252
           +  ENKA IVQ+G++++L++LMDP+AGMV+KAV VLANLA++ EGR  I +EGGI LLV+
Sbjct: 697 IHQENKAMIVQSGAVRYLIDLMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVE 756

Query: 253 TVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLS 312
            VELGSAR KE AAAALL +   + RFC M +QEGA+PPLV LSQSGT RA++KA+ALLS
Sbjct: 757 VVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLS 816

Query: 313 CFSRNK 319
            F   +
Sbjct: 817 YFRNQR 822

BLAST of Moc01g25840 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 337.4 bits (864), Expect = 1.2e-92
Identity = 191/306 (62.42%), Postives = 240/306 (78.43%), Query Frame = 0

Query: 13  QQPSERFVPEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSV 72
           ++PSER    IVS+   E R DL+ ++ +V+KLVEEL S SL+T R  TAELRLL K ++
Sbjct: 514 RRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNM 573

Query: 73  KNRRVIADSGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKIL 132
            NR VI +SGAI LLV LL S D+  QENAVTAL+NLSI+++ K  I  A AIEPL+ +L
Sbjct: 574 DNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVL 633

Query: 133 QTGSPEAKENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLS 192
           + GS EAKENSAATL SLSVI++N++ IG+SGAI  LVDLL +GTP+GKKDAATALFNLS
Sbjct: 634 ENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLS 693

Query: 193 LLTENKAKIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVD 252
           +  ENKA IVQ+G++++L++LMDP+AGMV+KAV VLANLA++ EGR  I +EGGI LLV+
Sbjct: 694 IHQENKAMIVQSGAVRYLIDLMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVE 753

Query: 253 TVELGSARAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLS 312
            VELGSAR KE AAAALL +   + RFC M +QEGA+PPLV LSQSGT RA++KA+ALLS
Sbjct: 754 VVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLS 813

Query: 313 CFSRNK 319
            F   +
Sbjct: 814 YFRNQR 819

BLAST of Moc01g25840 vs. TAIR 10
Match: AT5G67340.1 (ARM repeat superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 8.1e-76
Identity = 165/299 (55.18%), Postives = 221/299 (73.91%), Query Frame = 0

Query: 21  PEIVSSSVLEIRSDLTGIKAKVRKLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIAD 80
           P I+ ++V E  S  + I+ +V+KL+++L S SL+T R  TA +R+L + S  NR VIA 
Sbjct: 403 PGIIPATVRETGSS-SSIETEVKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIAR 462

Query: 81  SGAISLLVNLLNSRDTKIQENAVTALVNLSIDNDCKTMIVQANAIEPLVKILQTG-SPEA 140
             AI  LV+LL S D +IQ +AVT L+NLSI+++ K++I ++ AI PL+ +L+TG   EA
Sbjct: 463 CEAIPSLVSLLYSTDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEA 522

Query: 141 KENSAATLGSLSVIDDNQVNIGRSGAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKA 200
           K NSAATL SLSVI++ +  IG +GAIE LVDLL  G+  GKKDAATALFNLS+  ENK 
Sbjct: 523 KANSAATLFSLSVIEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKT 582

Query: 201 KIVQAGSIKHLVELMDPSAGMVEKAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSA 260
           K+++AG++++LVELMDP+ GMVEKAVVVLANLA+V EG+I I  EGGI +LV+ VELGSA
Sbjct: 583 KVIEAGAVRYLVELMDPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSA 642

Query: 261 RAKEYAAAALLWICATASRFCIMAIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNK 319
           R KE A AALL +C  + +FC   I+EG IPPLV L++SGT R K+KA+ LL  F  ++
Sbjct: 643 RGKENATAALLQLCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKAHR 700

BLAST of Moc01g25840 vs. TAIR 10
Match: AT3G54790.1 (ARM repeat superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 160/276 (57.97%), Postives = 204/276 (73.91%), Query Frame = 0

Query: 44  KLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAV 103
           KLVE+L S S +      AE+R LT  S++NR  I   GAI+ L++LL S +   QE+AV
Sbjct: 476 KLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAV 535

Query: 104 TALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRS 163
           TAL+NLSI    K MIV+  AIEPLV +L TG+  AKENSAA+L SLSV+  N+  IG+S
Sbjct: 536 TALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQS 595

Query: 164 -GAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVE 223
             AI+ALV+LL  GT +GKKDAA+ALFNLS+  +NKA+IVQA ++K+LVEL+DP   MV+
Sbjct: 596 NAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELLDPDLEMVD 655

Query: 224 KAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIM 283
           KAV +LANL++V EGR  IVREGGI LLV+TV+LGS R KE AA+ LL +C  + +FC +
Sbjct: 656 KAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTL 715

Query: 284 AIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNK 319
            +QEGAIPPLV LSQSGT RAK+KA+ LLS F   +
Sbjct: 716 VLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 751

BLAST of Moc01g25840 vs. TAIR 10
Match: AT3G54790.2 (ARM repeat superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 160/276 (57.97%), Postives = 204/276 (73.91%), Query Frame = 0

Query: 44  KLVEELTSDSLETLRTTTAELRLLTKLSVKNRRVIADSGAISLLVNLLNSRDTKIQENAV 103
           KLVE+L S S +      AE+R LT  S++NR  I   GAI+ L++LL S +   QE+AV
Sbjct: 440 KLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAV 499

Query: 104 TALVNLSIDNDCKTMIVQANAIEPLVKILQTGSPEAKENSAATLGSLSVIDDNQVNIGRS 163
           TAL+NLSI    K MIV+  AIEPLV +L TG+  AKENSAA+L SLSV+  N+  IG+S
Sbjct: 500 TALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQS 559

Query: 164 -GAIEALVDLLRDGTPQGKKDAATALFNLSLLTENKAKIVQAGSIKHLVELMDPSAGMVE 223
             AI+ALV+LL  GT +GKKDAA+ALFNLS+  +NKA+IVQA ++K+LVEL+DP   MV+
Sbjct: 560 NAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELLDPDLEMVD 619

Query: 224 KAVVVLANLASVDEGRIKIVREGGISLLVDTVELGSARAKEYAAAALLWICATASRFCIM 283
           KAV +LANL++V EGR  IVREGGI LLV+TV+LGS R KE AA+ LL +C  + +FC +
Sbjct: 620 KAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTL 679

Query: 284 AIQEGAIPPLVVLSQSGTTRAKDKARALLSCFSRNK 319
            +QEGAIPPLV LSQSGT RAK+KA+ LLS F   +
Sbjct: 680 VLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 715

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133100.15.3e-162100.00U-box domain-containing protein 4-like isoform X1 [Momordica charantia] >XP_0221... [more]
XP_008440337.12.1e-13483.70PREDICTED: U-box domain-containing protein 4 [Cucumis melo] >XP_008440338.1 PRED... [more]
XP_031742786.17.4e-13283.07U-box domain-containing protein 4 [Cucumis sativus] >KGN48575.1 hypothetical pro... [more]
TYK12846.11.5e-12783.33U-box domain-containing protein 4 [Cucumis melo var. makuwa][more]
XP_038880844.12.5e-12785.52U-box domain-containing protein 4-like [Benincasa hispida] >XP_038880845.1 U-box... [more]
Match NameE-valueIdentityDescription
O221931.7e-9162.42U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q5XEZ81.1e-7455.18U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Q8GWV52.2e-7057.97U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 S... [more]
Q5VRH96.5e-5447.76U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9C9A61.8e-5145.64U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1BVN72.6e-162100.00U-box domain-containing protein 4-like isoform X1 OS=Momordica charantia OX=3673... [more]
A0A1S3B0V31.0e-13483.70U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103484819 PE=4 S... [more]
A0A0A0KFV93.6e-13283.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493830 PE=4 SV=1[more]
A0A5D3CR207.0e-12883.33U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1BY411.6e-12481.88U-box domain-containing protein 4-like isoform X2 OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT2G23140.11.2e-9262.42RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.21.2e-9262.42RING/U-box superfamily protein with ARM repeat domain [more]
AT5G67340.18.1e-7655.18ARM repeat superfamily protein [more]
AT3G54790.11.6e-7157.97ARM repeat superfamily protein [more]
AT3G54790.21.6e-7157.97ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 234..274
e-value: 16.0
score: 11.6
coord: 153..193
e-value: 0.26
score: 20.4
coord: 112..152
e-value: 1.4E-4
score: 31.2
coord: 71..111
e-value: 8.7E-6
score: 35.2
coord: 194..233
e-value: 2.2E-4
score: 30.6
IPR000225ArmadilloPFAMPF00514Armcoord: 72..110
e-value: 6.2E-11
score: 42.0
coord: 155..192
e-value: 5.8E-6
score: 26.2
coord: 112..151
e-value: 8.3E-8
score: 32.0
coord: 196..233
e-value: 7.5E-5
score: 22.6
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 82..124
score: 13.5974
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 123..165
score: 8.627399
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 205..246
score: 11.4274
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 164..206
score: 11.2874
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 218..320
e-value: 4.3E-17
score: 63.8
coord: 22..217
e-value: 5.3E-37
score: 129.5
NoneNo IPR availablePANTHERPTHR23315:SF280RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 22..317
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 22..317
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 42..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g25840.1Moc01g25840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity