MS023029 (gene) Bitter gourd (TR) v1

BLAST of MS023029 vs. NCBI nr
Match: XP_022154283.1 (uncharacterized protein LOC111021573 [Momordica charantia])

HSP 1 Score: 962.6 bits (2487), Expect = 1.2e-276
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 60
           IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR
Sbjct: 16  IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 75

Query: 61  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 120
           CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN
Sbjct: 76  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 135

Query: 121 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 180
           ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS
Sbjct: 136 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 195

Query: 181 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 240
           NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA
Sbjct: 196 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 255

Query: 241 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 300
           ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD
Sbjct: 256 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 315

Query: 301 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 360
           GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN
Sbjct: 316 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 375

Query: 361 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 420
           GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL
Sbjct: 376 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 435

Query: 421 EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY 464
           EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY
Sbjct: 436 EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY 478

BLAST of MS023029 vs. NCBI nr
Match: XP_022931747.1 (uncharacterized protein LOC111437911 [Cucurbita moschata])

HSP 1 Score: 846.7 bits (2186), Expect = 9.7e-242
Identity = 403/462 (87.23%), Postives = 429/462 (92.86%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRC 61
           Q PMAIL FF  +++    + P  ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ C
Sbjct: 5   QRPMAILFFFFSLIILSHCVVP-SIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINNC 64

Query: 62  PSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENA 121
           PSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAENA
Sbjct: 65  PSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAENA 124

Query: 122 SDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSN 181
           SDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVSN
Sbjct: 125 SDVEITGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVSN 184

Query: 182 VTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAI 241
           V+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDAI
Sbjct: 185 VSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAI 244

Query: 242 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDG 301
           CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDG
Sbjct: 245 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDG 304

Query: 302 GSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENG 361
           GSANDITFSN+NI+TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI FINITA +ENG
Sbjct: 305 GSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINITATSENG 364

Query: 362 VFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLE 421
           VFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL 
Sbjct: 365 VFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLS 424

Query: 422 FENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
           FENVDMKWS  DD SL+WNNPLDFRPSTV+NISFFNFHSG L
Sbjct: 425 FENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFFNFHSGYL 465

BLAST of MS023029 vs. NCBI nr
Match: KAG7027765.1 (Exo-poly-alpha-D-galacturonosidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 403/462 (87.23%), Postives = 429/462 (92.86%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRC 61
           Q PMAIL FF  +++    + P  ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ C
Sbjct: 6   QHPMAILFFFFSLIIHSHCVVP-SIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINSC 65

Query: 62  PSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENA 121
           PSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAENA
Sbjct: 66  PSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAENA 125

Query: 122 SDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSN 181
           SDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVSN
Sbjct: 126 SDVEITGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVSN 185

Query: 182 VTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAI 241
           V+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDAI
Sbjct: 186 VSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAI 245

Query: 242 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDG 301
           CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDG
Sbjct: 246 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDG 305

Query: 302 GSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENG 361
           GSANDITFSN+NI+TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI FINITA +ENG
Sbjct: 306 GSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINITATSENG 365

Query: 362 VFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLE 421
           VFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL 
Sbjct: 366 VFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLS 425

Query: 422 FENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
           FENVDMKWS  DD SL+WNNPLDFRPSTV+NISFFNFHSG L
Sbjct: 426 FENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFFNFHSGYL 466

BLAST of MS023029 vs. NCBI nr
Match: XP_023538800.1 (uncharacterized protein LOC111799618 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.3 bits (2180), Expect = 4.8e-241
Identity = 401/462 (86.80%), Postives = 428/462 (92.64%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRC 61
           Q PMAIL FF  +++    + P  ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ C
Sbjct: 5   QHPMAILFFFFSLIIHSHCVVP-SIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINSC 64

Query: 62  PSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENA 121
           PSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAENA
Sbjct: 65  PSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAENA 124

Query: 122 SDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSN 181
           SDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVSN
Sbjct: 125 SDVEISGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVSN 184

Query: 182 VTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAI 241
           V+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDAI
Sbjct: 185 VSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAI 244

Query: 242 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDG 301
           CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDG
Sbjct: 245 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDG 304

Query: 302 GSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENG 361
           GSANDITFSN+N++TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI FINITA +ENG
Sbjct: 305 GSANDITFSNMNVSTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINITATSENG 364

Query: 362 VFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLE 421
           VFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL 
Sbjct: 365 VFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLS 424

Query: 422 FENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
           FENVDMKWS  DD SL+WNNPLDFRPSTV+NISF NFHSG L
Sbjct: 425 FENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYL 465

BLAST of MS023029 vs. NCBI nr
Match: XP_022971216.1 (uncharacterized protein LOC111470006 [Cucurbita maxima])

HSP 1 Score: 840.5 bits (2170), Expect = 7.0e-240
Identity = 402/463 (86.83%), Postives = 429/463 (92.66%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIP-LQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 61
           Q PMAILLFF   ++ + S   +P ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ 
Sbjct: 5   QHPMAILLFFFFSLI-IHSRCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINS 64

Query: 62  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 121
           CPSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAEN
Sbjct: 65  CPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAEN 124

Query: 122 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 181
           ASDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVS
Sbjct: 125 ASDVEIIGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVS 184

Query: 182 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 241
           NV+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDA
Sbjct: 185 NVSLNQPAHWCLHLVXCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 244

Query: 242 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 301
           ICPK SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRD
Sbjct: 245 ICPKASNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 304

Query: 302 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 361
           GGSANDITFSN+NI+TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISN+ FINITA +EN
Sbjct: 305 GGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNVNFINITATSEN 364

Query: 362 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 421
           GVFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL
Sbjct: 365 GVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 424

Query: 422 EFENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
            FENVDMKWS  DD SL+WNNPLDFRPSTV+NISF NFHSG L
Sbjct: 425 SFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYL 466

BLAST of MS023029 vs. ExPASy Swiss-Prot
Match: P15922 (Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.9e-23
Identity = 91/329 (27.66%), Postives = 149/329 (45.29%), Query Frame = 0

Query: 36  SVADFGAIGDGVHYDTAAIQSAIDRCPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGA 95
           ++  +GA GDG   +T+AIQ AID C  P+ C +  P G + T  +WL+S + L++  GA
Sbjct: 153 NITQYGAKGDGTTLNTSAIQKAIDAC--PTGCRIDVPAGVFKTGALWLKSDMTLNLLQGA 212

Query: 96  TVLAGTRIEDYPAESSRWYAVVAE------------NASDVG------IGGGGAVDGQGL 155
           T+L      DYP ++ + Y+ V++            N+S VG      I G G +DG G 
Sbjct: 213 TLLGSDNAADYP-DAYKIYSYVSQVRPASLLNAIDKNSSAVGTFKNIRIVGKGIIDGNGW 272

Query: 156 K---------------FVEKFDKR--KNVMVSWNQTGACYG---------DECRPDLVGF 215
           K               +V+  + +  K+ +++ NQ  A             + R  LV  
Sbjct: 273 KRSADAKDELGNTLPQYVKSDNSKVSKDGILAKNQVAAAVATGMDTKTAYSQRRSSLVTL 332

Query: 216 IASKNVRVSNVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITR 275
              +N  +++VT+  PA+  +  +  EN ++E+  I+  F++ N DG++  +S N  +  
Sbjct: 333 RGVQNAYIADVTIRNPANHGIMFLESEN-VVENSVIHQTFNANNGDGVEFGNSQNIMVFN 392

Query: 276 CRIDTGDDAICPKTSNG-------PVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDN 312
              DTGDD+I      G       P  N    N + R    A+ LGS +      +L +N
Sbjct: 393 SVFDTGDDSINFAAGMGQDAQKQEPSQNAWLFNNFFRHGHGAVVLGSHTGAGIVDVLAEN 452

BLAST of MS023029 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.7e-16
Identity = 91/347 (26.22%), Postives = 146/347 (42.07%), Query Frame = 0

Query: 29  PGGSTYFSVADFGAIGDGVHYDTAAIQSA-IDRCPSPSRCYVTFPPGTYLTATIWLRSGV 88
           PGGS  F +   GA G+G    T A+Q A    C    +  +  P G +L   +      
Sbjct: 37  PGGS--FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPC 96

Query: 89  VLD--IQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGIGGGGAVDGQGLKFVEKFDK 148
             D  IQ    +LA T +  Y  E   W  ++     ++ I G G +DGQG         
Sbjct: 97  KGDVTIQVDGNLLATTDLSQY-KEHGNWIEIL--RVDNLVITGKGNLDGQG--------- 156

Query: 149 RKNVMVSWNQTGACYGDECR--PDLVGFIASKNVRVSNVTLNQPAHWCLHLVGCENTIIE 208
                  W++       +C+  P+ +      N  VS VTL     + +++  C+N +I+
Sbjct: 157 ----PAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIK 216

Query: 209 DVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAIC--PKTSNGPVFNLTATNCWIR 268
           DV++    DSPN DGI + DS+   IT   I  GDD I   P TS     N+T   C   
Sbjct: 217 DVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSK---VNITGVTC--- 276

Query: 269 TKSSAIKLGSASWF----NFTHMLFDNITIVDSHRGLGFQLRDGGSANDITFSNINITTR 328
                I +GS   +    + T +   + T+  +  G+  +  +  +A+ +T S I+    
Sbjct: 277 GPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYE-DAASVLTVSKIH---- 336

Query: 329 YYDPSWWGRAEPIYVTT--CPRD----PGSKEGTISNIQFINITAVA 359
           Y +      A PI++    CP       G+ + T+ ++ F NIT  +
Sbjct: 337 YENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTS 354

BLAST of MS023029 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.0e-15
Identity = 79/304 (25.99%), Postives = 122/304 (40.13%), Query Frame = 0

Query: 23  PIPLQLPGGS---TYFSVADFGAIGDGVHYDTAAIQSAIDRCPSPSRCYVTFPPGTYLTA 82
           P P   PG S     F V  FGA+GDG   DTAA Q A     +     V  P G     
Sbjct: 65  PSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKI 124

Query: 83  TIWLRSGVV---LDIQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGIGGGGAVDGQG 142
           T  + SG     L  Q    ++     E++P + ++   +V          G G V+G G
Sbjct: 125 TSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNG 184

Query: 143 LKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVG 202
            K+ +   K        + +G C      P ++ F  S N+ V  + +     + +   G
Sbjct: 185 QKWWDLPCKPHRGPDGSSSSGPC----ASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 244

Query: 203 CENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTSNGPVFNLTAT 262
           C+  +I ++ I     SPN DGI + ++ +  I    +  GDD I   T    V ++   
Sbjct: 245 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDV-DIQGV 304

Query: 263 NCWIRTKSSAIKLGSASWFN----FTHMLFDNITIVDSHRGLGFQLRDG--GSANDITFS 315
            C     S  I +GS    N     +++   N  I DS  GL  +   G  GS +++ F 
Sbjct: 305 TC---GPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 360

BLAST of MS023029 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 2.9e-15
Identity = 83/289 (28.72%), Postives = 125/289 (43.25%), Query Frame = 0

Query: 33  TYFSVADFGAIGDGVHYDTAAIQSAIDR-CPSPSRCYVTFPPG-TYLTATIWLRS--GVV 92
           T  SV+DFGA GDG   DT A  +A  + C S     +  P G TYL  +I L      +
Sbjct: 66  TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 125

Query: 93  LDIQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGIGGGGAVDGQGLKFVEKFDKRKN 152
           L +Q   T+ A  +  DY  + S+W      N   V  G  G VDG G  + +   KR  
Sbjct: 126 LTVQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKR-- 185

Query: 153 VMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVGCENTIIEDVSIY 212
                N+   C      P  + F  SK++ V N+ +       + +  C N  + +V + 
Sbjct: 186 -----NKAKPC---TKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVT 245

Query: 213 GDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTSNGPVFNLTATNCWIRTKSSAIK 272
              DSPN DGI I ++ N  ++   I TGDD I  ++ +  V  +    C      S   
Sbjct: 246 APADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNV-QINDITCGPGHGISIGS 305

Query: 273 LGSASWFNF-THMLFDNITIVDSHRGLGFQLRDGGS--ANDITFSNINI 315
           LG  +   F + +  D   +  +  G+  +   GGS  A++I F NI +
Sbjct: 306 LGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQM 342

BLAST of MS023029 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.6e-15
Identity = 89/347 (25.65%), Postives = 146/347 (42.07%), Query Frame = 0

Query: 29  PGGSTYFSVADFGAIGDGVHYDTAAIQSA-IDRCPSPSRCYVTFPPGTYLTATIWLRSGV 88
           PGGS  F +   GA G+G    T A+Q A    C    +  +  P G +L   +      
Sbjct: 37  PGGS--FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPC 96

Query: 89  VLD--IQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGIGGGGAVDGQGLKFVEKFDK 148
             D  IQ    +LA T +  Y  +   W  ++     ++ I G G +DGQG         
Sbjct: 97  KGDVTIQVDGNLLATTDLSQY-KDHGNWIEIL--RVDNLVITGKGNLDGQG--------- 156

Query: 149 RKNVMVSWNQTGACYGDECR--PDLVGFIASKNVRVSNVTLNQPAHWCLHLVGCENTIIE 208
                  W++       +C+  P+ +      N  VS VTL     + +++  C++ +I+
Sbjct: 157 ----PAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIK 216

Query: 209 DVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAIC--PKTSNGPVFNLTATNCWIR 268
           DV++    DSPN DGI + DS+   IT   I  GDD I   P TS     N+T   C   
Sbjct: 217 DVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSK---VNITGVTC--- 276

Query: 269 TKSSAIKLGSASWF----NFTHMLFDNITIVDSHRGLGFQLRDGGSANDITFSNINITTR 328
                I +GS   +    + T +   + T+  +  G+  +  +  +A+ +T S I+    
Sbjct: 277 GPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYE-DAASVLTVSKIH---- 336

Query: 329 YYDPSWWGRAEPIYVTT--CPRD----PGSKEGTISNIQFINITAVA 359
           Y +      A PI++    CP       G+ + T+ ++ F NIT  +
Sbjct: 337 YENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTS 354

BLAST of MS023029 vs. ExPASy TrEMBL
Match: A0A6J1DJW9 (uncharacterized protein LOC111021573 OS=Momordica charantia OX=3673 GN=LOC111021573 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 5.9e-277
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 60
           IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR
Sbjct: 16  IQLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 75

Query: 61  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 120
           CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN
Sbjct: 76  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 135

Query: 121 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 180
           ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS
Sbjct: 136 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 195

Query: 181 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 240
           NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA
Sbjct: 196 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 255

Query: 241 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 300
           ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD
Sbjct: 256 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 315

Query: 301 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 360
           GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN
Sbjct: 316 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 375

Query: 361 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 420
           GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL
Sbjct: 376 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 435

Query: 421 EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY 464
           EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY
Sbjct: 436 EFENVDMKWSDDRSLKWNNPLDFRPSTVNNISFFNFHSGLLTY 478

BLAST of MS023029 vs. ExPASy TrEMBL
Match: A0A6J1EUK0 (uncharacterized protein LOC111437911 OS=Cucurbita moschata OX=3662 GN=LOC111437911 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.7e-242
Identity = 403/462 (87.23%), Postives = 429/462 (92.86%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRC 61
           Q PMAIL FF  +++    + P  ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ C
Sbjct: 5   QRPMAILFFFFSLIILSHCVVP-SIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINNC 64

Query: 62  PSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENA 121
           PSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAENA
Sbjct: 65  PSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAENA 124

Query: 122 SDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSN 181
           SDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVSN
Sbjct: 125 SDVEITGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVSN 184

Query: 182 VTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAI 241
           V+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDAI
Sbjct: 185 VSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAI 244

Query: 242 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDG 301
           CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDG
Sbjct: 245 CPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDG 304

Query: 302 GSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENG 361
           GSANDITFSN+NI+TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI FINITA +ENG
Sbjct: 305 GSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINITATSENG 364

Query: 362 VFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLE 421
           VFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL 
Sbjct: 365 VFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLS 424

Query: 422 FENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
           FENVDMKWS  DD SL+WNNPLDFRPSTV+NISFFNFHSG L
Sbjct: 425 FENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFFNFHSGYL 465

BLAST of MS023029 vs. ExPASy TrEMBL
Match: A0A6J1I2Q7 (uncharacterized protein LOC111470006 OS=Cucurbita maxima OX=3661 GN=LOC111470006 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.4e-240
Identity = 402/463 (86.83%), Postives = 429/463 (92.66%), Query Frame = 0

Query: 2   QLPMAILLFFLLIVLPVQSLRPIP-LQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDR 61
           Q PMAILLFF   ++ + S   +P ++LPGGST FSV DFGAIGDGVHYDTAAIQSAI+ 
Sbjct: 5   QHPMAILLFFFFSLI-IHSRCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSAINS 64

Query: 62  CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAEN 121
           CPSP RCYV FPPGTYLTATIWL+SGVVLDIQPGATVLAGTR+EDYP +SSRWYAVVAEN
Sbjct: 65  CPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVVAEN 124

Query: 122 ASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVS 181
           ASDV I GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDL+GFI SKNVRVS
Sbjct: 125 ASDVEIIGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNVRVS 184

Query: 182 NVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDA 241
           NV+LNQPAHWCLHLV CENTIIEDVSIYGDFD+PNNDGIDIEDSNNTFITRCRIDTGDDA
Sbjct: 185 NVSLNQPAHWCLHLVXCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 244

Query: 242 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD 301
           ICPK SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRD
Sbjct: 245 ICPKASNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 304

Query: 302 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 361
           GGSANDITFSN+NI+TRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISN+ FINITA +EN
Sbjct: 305 GGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNVNFINITATSEN 364

Query: 362 GVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGL 421
           GVFLSGS+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL
Sbjct: 365 GVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 424

Query: 422 EFENVDMKWS--DDRSLKWNNPLDFRPSTVNNISFFNFHSGLL 462
            FENVDMKWS  DD SL+WNNPLDFRPSTV+NISF NFHSG L
Sbjct: 425 SFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYL 466

BLAST of MS023029 vs. ExPASy TrEMBL
Match: A0A5D3DP49 (Exo-poly-alpha-D-galacturonosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005690 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 395/454 (87.00%), Postives = 420/454 (92.51%), Query Frame = 0

Query: 8   LLFFLLIVLPVQSLRPIP-LQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRCPSPSR 67
           L FF   +L + S   IP ++L   ST FSV DFGAIGDG+HYDTAAIQSAI+ CP+PSR
Sbjct: 9   LFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINSCPAPSR 68

Query: 68  CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGI 127
           CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT++EDYP +SSRWYAVVAENASDVGI
Sbjct: 69  CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAENASDVGI 128

Query: 128 GGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQ 187
            GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDLVGFI S NVRVSNV+ NQ
Sbjct: 129 SGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVSNVSFNQ 188

Query: 188 PAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTS 247
           PAHWCLHLV CENT+IEDVSIYGDFD+PNNDGIDIEDSNNT ITRCRIDTGDDAICPK+S
Sbjct: 189 PAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDAICPKSS 248

Query: 248 NGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDGGSAND 307
           NGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDGGSAND
Sbjct: 249 NGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDGGSAND 308

Query: 308 ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENGVFLSG 367
           ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI+FINITA +ENGVFLSG
Sbjct: 309 ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSENGVFLSG 368

Query: 368 SRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLEFENVD 427
           S+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL  ENVD
Sbjct: 369 SKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLNLENVD 428

Query: 428 MKWSD-DRSLKWNNPLDFRPSTVNNISFFNFHSG 460
           M WSD D SL+WNNPLDFRPSTVNNISFFNFHSG
Sbjct: 429 MNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSG 462

BLAST of MS023029 vs. ExPASy TrEMBL
Match: A0A1S3B7Z6 (exo-poly-alpha-D-galacturonosidase OS=Cucumis melo OX=3656 GN=LOC103486792 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 395/454 (87.00%), Postives = 420/454 (92.51%), Query Frame = 0

Query: 8   LLFFLLIVLPVQSLRPIP-LQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRCPSPSR 67
           L FF   +L + S   IP ++L   ST FSV DFGAIGDG+HYDTAAIQSAI+ CP+PSR
Sbjct: 9   LFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINSCPAPSR 68

Query: 68  CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIEDYPAESSRWYAVVAENASDVGI 127
           CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT++EDYP +SSRWYAVVAENASDVGI
Sbjct: 69  CYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAENASDVGI 128

Query: 128 GGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQ 187
            GGG VDGQGLKFVEKFDKRKNVMVSWN+TGACYGDECRPDLVGFI S NVRVSNV+ NQ
Sbjct: 129 SGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVSNVSFNQ 188

Query: 188 PAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTS 247
           PAHWCLHLV CENT+IEDVSIYGDFD+PNNDGIDIEDSNNT ITRCRIDTGDDAICPK+S
Sbjct: 189 PAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDAICPKSS 248

Query: 248 NGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDGGSAND 307
           NGPVFNLTATNCWIRTKSSAIKLGSASWFNFT MLFDNITIVDSHRGLGFQLRDGGSAND
Sbjct: 249 NGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDGGSAND 308

Query: 308 ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENGVFLSG 367
           ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEG+ISNI+FINITA +ENGVFLSG
Sbjct: 309 ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSENGVFLSG 368

Query: 368 SRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKHGMAGMTMEHIEGLEFENVD 427
           S+SGV+SNLRF+NV L +KRWT+YGGG+ADYRPGCQGFVKHGMAGM MEHIEGL  ENVD
Sbjct: 369 SKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGLNLENVD 428

Query: 428 MKWSD-DRSLKWNNPLDFRPSTVNNISFFNFHSG 460
           M WSD D SL+WNNPLDFRPSTVNNISFFNFHSG
Sbjct: 429 MNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSG 462

BLAST of MS023029 vs. TAIR 10
Match: AT3G57790.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 637.9 bits (1644), Expect = 6.4e-183
Identity = 305/466 (65.45%), Postives = 364/466 (78.11%), Query Frame = 0

Query: 7   ILLFFLLIVLPVQSLRPIPLQLPGGSTYFSVADFGAIGDGVHYDTAAIQSAIDRC----- 66
           +LL F  +V          +QLPG S   SV DFGA GDG++YDT+AIQS ID C     
Sbjct: 7   LLLLFFSLVQSRSDTSYSKIQLPGDSLTLSVTDFGATGDGINYDTSAIQSTIDACNRHYT 66

Query: 67  PSPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRIED-YPAE-SSRWYAVVAE 126
              S C V FP G YLTA + LRSGV+LD+   A +L G RIED YPAE SS WY VVA 
Sbjct: 67  SFSSICRVVFPSGNYLTAKLHLRSGVILDVTENAVLLGGPRIEDYYPAETSSDWYVVVAN 126

Query: 127 NASDVGIGGGGAVDGQGLKFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRV 186
           NA+DVGI GGGA+DGQG KFV +FD++KNVMVSWNQTGAC GDECRP LVGF+ S NV +
Sbjct: 127 NATDVGITGGGAIDGQGSKFVVRFDEKKNVMVSWNQTGACLGDECRPRLVGFVDSINVEI 186

Query: 187 SNVTLNQPAHWCLHLVGCENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDD 246
            N+TL +PA+WCLH+V CENT + DVSI GDF++PNNDGIDIEDSNNT ITRC IDTGDD
Sbjct: 187 WNITLREPAYWCLHIVRCENTSVHDVSILGDFNTPNNDGIDIEDSNNTVITRCHIDTGDD 246

Query: 247 AICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLR 306
           AICPKT  GP++NLTAT+CWIRTKSSAIKLGSASWF+F  ++FDNITI +SHRGLG Q+R
Sbjct: 247 AICPKTYTGPLYNLTATDCWIRTKSSAIKLGSASWFDFKGLVFDNITIFESHRGLGMQIR 306

Query: 307 DGGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAE 366
           DGG+ +D+TFSNINI+TRYYDPSWWGRAEPIY+TTCPRD  +KEG+ISN+ F+NIT  +E
Sbjct: 307 DGGNVSDVTFSNINISTRYYDPSWWGRAEPIYITTCPRDSSAKEGSISNLLFVNITIDSE 366

Query: 367 NGVFLSGSRSGVVSNLRFSNVSLAFKRWTEYGGGLADYRPGCQGFVKH-GMAGMTMEHIE 426
           NGVFLSGS +G++S+++F N++L F+RW+ Y  GL DYRPGCQG V H   +G+ MEH+ 
Sbjct: 367 NGVFLSGSPNGLLSDIKFKNMNLTFRRWSNYSAGLVDYRPGCQGLVNHRATSGIIMEHVN 426

Query: 427 GLEFENVDMKWSDDRSLK--WNNPLDFRPSTVNNISFFNFHSGLLT 463
           G   ENVD+KWSDD  +   WN PL+FRPSTVNN+SF  F SGL T
Sbjct: 427 GFRVENVDLKWSDDDDVNAAWNVPLEFRPSTVNNVSFVGFTSGLYT 472

BLAST of MS023029 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-26
Identity = 103/381 (27.03%), Postives = 168/381 (44.09%), Query Frame = 0

Query: 37  VADFGAIGDGVHYDTAAIQSAIDR---CPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQP 96
           + DFGA+GDG   +T A ++AI +     +     +  PPG +LT +  L S   L IQ 
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 97  GATVLAGTRIEDYP--------------AESSRWYAVVA-ENASDVGI-GGGGAVDGQGL 156
           GAT+LA     ++P                + R+ ++++  N +DV I G  G ++GQG 
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 157 KFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVGC 216
            + +KF K+K   ++            RP L+  + SKN+++SN+TL     W +H V C
Sbjct: 167 YWWDKF-KKKQFKIT------------RPYLIEILFSKNIQISNITLIDSPSWNIHPVYC 226

Query: 217 ENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKT---SNGPVFNLT 276
            + I++ V++      PN DGI+ +   NT I  C I +GDD I  K+     G  F + 
Sbjct: 227 NSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMP 286

Query: 277 ATNCWIR------TKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRDGGSA--ND 336
                IR       KS+ + LGS        +  +++T+ ++   +  +   G  A   D
Sbjct: 287 TQQLSIRRLTCISPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKD 346

Query: 337 ITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGTISNIQFINITAVAENGVFLSG 384
           I    I + T  Y   +W             D G     +  I  IN   +    V +S 
Sbjct: 347 IYARRITMKTMKY--VFWMSGN----YGSHPDEGFDPKALPEITNINYRDMTAENVTMSA 406

BLAST of MS023029 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 3.6e-24
Identity = 85/306 (27.78%), Postives = 143/306 (46.73%), Query Frame = 0

Query: 36  SVADFGAIGDGVHYDTAAIQSAI---DRCPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQ 95
           S+ DFG +GDG   +TAA + A+   +   +     +  P GT+L+ +  L S   L ++
Sbjct: 73  SITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLE 132

Query: 96  PGATVLAGTRIEDYP-------------AESSRWYAVV-AENASDVGI-GGGGAVDGQGL 155
            GA +L    ++++P                 R  +++  +N ++V I G  G +DGQG 
Sbjct: 133 RGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGK 192

Query: 156 KFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVGC 215
            + E +         WN+T        R  L+    S N+ +SN+TL     W +H V C
Sbjct: 193 MWWELW---------WNRTLV----HTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYC 252

Query: 216 ENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKT---SNGPVFNLT 275
            N +I +++I    ++PN DGID + S N  I  C I++GDD +  K+     G      
Sbjct: 253 SNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARP 312

Query: 276 ATNCWIRTKS------SAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD--GGSAND 313
           ++N  IR  S      S + +GS       ++  ++I + DS  GL  +     GG  ++
Sbjct: 313 SSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISN 365

BLAST of MS023029 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 3.6e-24
Identity = 94/314 (29.94%), Postives = 141/314 (44.90%), Query Frame = 0

Query: 36  SVADFGAIGDGVHYDTAAIQSAIDRCPSPSR---CYVTFPPGTYLTATIWLRSGVVLDIQ 95
           S+ DFG +GDG   +T A ++AI R     R     +  PPG YLT +  L S + L + 
Sbjct: 42  SITDFGGVGDGRTVNTKAFRAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLA 101

Query: 96  PGATVLAGTRIEDYP-------------AESSRWYAVV-AENASDVGI-GGGGAVDGQGL 155
            GA + A     ++P                 R+ + +  +   DV I G  G +DGQG 
Sbjct: 102 KGAVIRAVQDTWNWPLIDPLPSYGRGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQG- 161

Query: 156 KFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVGC 215
                        V WN   +      RP+L+ F  SK + +SNV       W +H V C
Sbjct: 162 ------------EVWWNMWRSRTLKYTRPNLIEFKDSKEIIISNVIFQNSPFWNIHPVYC 221

Query: 216 ENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPKT---SNGPVFNLT 275
            N +I  V+I    DSPN DGID + S N  I    I TGDD +  K+     G  +   
Sbjct: 222 SNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDDLVAIKSGWDQYGIAYGRP 281

Query: 276 ATNCWIR--TKSS---AIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLRD----GGSAN 320
           ++N  IR  T SS    I +GS +     +++ ++IT+  S+ G+G  ++     GG   
Sbjct: 282 SSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHITL--SNMGVGVNIKTNIGRGGYIK 340

BLAST of MS023029 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-22
Identity = 97/386 (25.13%), Postives = 158/386 (40.93%), Query Frame = 0

Query: 36  SVADFGAIGDGVHYDTAAIQSA---IDRCPSPSRCYVTFPPGTYLTATIWLRSGVVLDIQ 95
           S+ +FGA+GDGV  +T A Q+A   ++         +  P G +LT +  L S + L + 
Sbjct: 44  SITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLD 103

Query: 96  PGATVLAGTRIEDYPAES--------------SRWYAVVAENASDVGI-GGGGAVDGQGL 155
            GAT+L  T  E++P                      +  +N +DV I G  G +DGQG 
Sbjct: 104 KGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQG- 163

Query: 156 KFVEKFDKRKNVMVSWNQTGACYGDECRPDLVGFIASKNVRVSNVTLNQPAHWCLHLVGC 215
                      V   W + G    +  RP LV  + S  + +SN+T      W +H V C
Sbjct: 164 ----------TVWWDWFRNGEL--NYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYC 223

Query: 216 ENTIIEDVSIYGDFDSPNNDGIDIEDSNNTFITRCRIDTGDDAICPK------------- 275
            + ++++++I    +SPN DG+D + S N  I  C I TGDD +  K             
Sbjct: 224 RDVVVKNLTILAPLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARP 283

Query: 276 TSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTHMLFDNITIVDSHRGLGFQLR--DGG 335
           +S   +  LT       T SS I +GS      + +   ++ + +S+ G+  +     GG
Sbjct: 284 SSKIKINRLTGQT----TSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGG 343

Query: 336 SANDITFSNINITTRYYDPSWWGR--AEPIYVTTCPRDPGSKEGTISNIQFINITAVAEN 387
              ++   N+ +        + G+    P         P  ++ T  N+    I      
Sbjct: 344 YVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAG-- 403