MS021822 (gene) Bitter gourd (TR) v1

BLAST of MS021822 vs. NCBI nr
Match: XP_022140916.1 (serine/threonine-protein kinase STY8-like isoform X2 [Momordica charantia])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 476/562 (84.70%), Postives = 482/562 (85.77%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH
Sbjct: 86  MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 145

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ       N   S+HS  
Sbjct: 146 RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQVHSSPGGNSGKSVHSNL 205

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR               G   NE        ++   T 
Sbjct: 206 KEKVDQRFSDYASN--------QRHHPPPAFGSSTEVEYGFKANELHD-----RDQSGTL 265

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 266 SWPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 325

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  +      +I        KRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW
Sbjct: 326 KLKDVLAEEISNI-------QKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 385

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EIDASLLIYEKKIVSGSFSDLY+GTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR
Sbjct: 386 EIDASLLIYEKKIVSGSFSDLYRGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 445

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH
Sbjct: 446 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 505

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 506 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 565

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ
Sbjct: 566 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 625

Query: 541 QDPSLRPEFSEITKLLQQTLKE 549
           QDPSLRPEFSEITKLLQQTLKE
Sbjct: 626 QDPSLRPEFSEITKLLQQTLKE 627

BLAST of MS021822 vs. NCBI nr
Match: XP_022140889.1 (serine/threonine-protein kinase STY8-like isoform X1 [Momordica charantia])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 476/562 (84.70%), Postives = 483/562 (85.94%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH
Sbjct: 86  MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 145

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ       N   S+HS  
Sbjct: 146 RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQVHSSPGGNSGKSVHSNL 205

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR               G   NE        ++   T 
Sbjct: 206 KEKVDQRFSDYASN--------QRHHPPPAFGSSTEVEYGFKANELHD-----RDQSGTL 265

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 266 SWPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 325

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  +      +I      + KRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW
Sbjct: 326 KLKDVLAEEISNI------QVKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 385

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EIDASLLIYEKKIVSGSFSDLY+GTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR
Sbjct: 386 EIDASLLIYEKKIVSGSFSDLYRGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 445

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH
Sbjct: 446 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 505

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 506 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 565

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ
Sbjct: 566 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 625

Query: 541 QDPSLRPEFSEITKLLQQTLKE 549
           QDPSLRPEFSEITKLLQQTLKE
Sbjct: 626 QDPSLRPEFSEITKLLQQTLKE 628

BLAST of MS021822 vs. NCBI nr
Match: XP_038897099.1 (serine/threonine-protein kinase STY8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 803.1 bits (2073), Expect = 1.5e-228
Identity = 428/565 (75.75%), Postives = 458/565 (81.06%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVD FS ++W+QKQ V VYDEVLRRLR+SHEAETS+PGFEDELWAHFH
Sbjct: 1   MAMDDTESCSSRAVDDFSAQTWKQKQMVGVYDEVLRRLRLSHEAETSLPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQNMMVSLHSVDQFSPSKQ 120
           RLPTRYAMDVNVERA+DVLMHKNLL+MAHDPA RPAIDVRLVQ     +HS    SP + 
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKNLLYMAHDPATRPAIDVRLVQ-----VHS----SPGR- 120

Query: 121 NIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHF-------------NTF 180
           N         +EK   L SG   Y     ++    + S  +  +              TF
Sbjct: 121 NFGKSVNSNLKEKVDQLFSG---YDSNQRHHPPPAFGSSTEVEYGFETSQLHDIDQPGTF 180

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 181 SWPMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 240

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  I       I        KRHP   P  + HTGKQD++GVKFIT  VNIP+DEVDVW
Sbjct: 241 QLKDILAEEISKI-------QKRHPPFTP-CIFHTGKQDKSGVKFITKYVNIPRDEVDVW 300

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EID SLL++EKKI SGS SDLYKGTFYGQDVAIKLLKDENLNET RREF QEIHIMRK+R
Sbjct: 301 EIDVSLLVFEKKIASGSLSDLYKGTFYGQDVAIKLLKDENLNETARREFFQEIHIMRKLR 360

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNV+QFIGA T+PPS  IVTEYMSGGS+HDF HQQKGVLSFPSLLRVAVDVSKGM+YLH
Sbjct: 361 HKNVLQFIGASTKPPSFLIVTEYMSGGSIHDFLHQQKGVLSFPSLLRVAVDVSKGMNYLH 420

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDE GVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 421 QKNIIHRDLKAANLLMDEYGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTG+LPYDNLTPLQAAIGVVQKGLRPK PRHA P IVDLL+KCW 
Sbjct: 481 HKADVYSFGIVLWELLTGQLPYDNLTPLQAAIGVVQKGLRPKIPRHADPRIVDLLEKCWL 540

Query: 541 QDPSLRPEFSEITKLLQQTLKEVGE 552
           QDPSLRPEFSEIT+ LQQTLKEVG+
Sbjct: 541 QDPSLRPEFSEITRYLQQTLKEVGK 544

BLAST of MS021822 vs. NCBI nr
Match: XP_038897101.1 (serine/threonine-protein kinase STY46-like isoform X3 [Benincasa hispida])

HSP 1 Score: 800.0 bits (2065), Expect = 1.2e-227
Identity = 426/563 (75.67%), Postives = 456/563 (80.99%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVD FS ++W+QKQ V VYDEVLRRLR+SHEAETS+PGFEDELWAHFH
Sbjct: 1   MAMDDTESCSSRAVDDFSAQTWKQKQMVGVYDEVLRRLRLSHEAETSLPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQNMMVSLHSVDQFSPSKQ 120
           RLPTRYAMDVNVERA+DVLMHKNLL+MAHDPA RPAIDVRLVQ     +HS    SP + 
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKNLLYMAHDPATRPAIDVRLVQ-----VHS----SPGR- 120

Query: 121 NIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHF-------------NTF 180
           N         +EK   L SG   Y     ++    + S  +  +              TF
Sbjct: 121 NFGKSVNSNLKEKVDQLFSG---YDSNQRHHPPPAFGSSTEVEYGFETSQLHDIDQPGTF 180

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 181 SWPMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 240

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  I       I        KRHP   P  + HTGKQD++GVKFIT  VNIP+DEVDVW
Sbjct: 241 QLKDILAEEISKI-------QKRHPPFTP-CIFHTGKQDKSGVKFITKYVNIPRDEVDVW 300

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EID SLL++EKKI SGS SDLYKGTFYGQDVAIKLLKDENLNET RREF QEIHIMRK+R
Sbjct: 301 EIDVSLLVFEKKIASGSLSDLYKGTFYGQDVAIKLLKDENLNETARREFFQEIHIMRKLR 360

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNV+QFIGA T+PPS  IVTEYMSGGS+HDF HQQKGVLSFPSLLRVAVDVSKGM+YLH
Sbjct: 361 HKNVLQFIGASTKPPSFLIVTEYMSGGSIHDFLHQQKGVLSFPSLLRVAVDVSKGMNYLH 420

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDE GVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 421 QKNIIHRDLKAANLLMDEYGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTG+LPYDNLTPLQAAIGVVQKGLRPK PRHA P IVDLL+KCW 
Sbjct: 481 HKADVYSFGIVLWELLTGQLPYDNLTPLQAAIGVVQKGLRPKIPRHADPRIVDLLEKCWL 540

Query: 541 QDPSLRPEFSEITKLLQQTLKEV 550
           QDPSLRPEFSEIT+ LQQTLKE+
Sbjct: 541 QDPSLRPEFSEITRYLQQTLKEI 542

BLAST of MS021822 vs. NCBI nr
Match: XP_023525869.1 (serine/threonine-protein kinase STY46-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 799.7 bits (2064), Expect = 1.6e-227
Identity = 421/561 (75.04%), Postives = 453/561 (80.75%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESC SR VD FST++WRQKQKV VY EVLRRLR+SHEAETS PGFEDELWAHFH
Sbjct: 1   MAMDDTESCCSRPVDDFSTQTWRQKQKVGVYVEVLRRLRLSHEAETSFPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERA+DVLMHK+LLFMAH PA RPAIDVRLVQ       N    +HS  
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKDLLFMAHAPATRPAIDVRLVQVHSSPGTNSGKYVHSNL 120

Query: 121 ---VDQFSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTFR 180
              VDQ  P   +        QR+              G   ++       + +      
Sbjct: 121 KEKVDQRFPDYDSN-------QRQHPPPAFGSTTEVESGFAISQ----LHDINQPDTLSW 180

Query: 181 PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILLL 240
           PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE    L
Sbjct: 181 PMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTKQL 240

Query: 241 FLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEI 300
             I       I        KRHP   PWS+S TGK+D+ GVKFITN VNIP+ +V  WEI
Sbjct: 241 KDILAEEISKI-------QKRHPLFTPWSISPTGKKDKIGVKFITNYVNIPRHKVGAWEI 300

Query: 301 DASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRHK 360
           DASLLIYEKKIVSGSFSDLYKGTFYGQ+VAIKLLKD +LNE VRREFAQEIHIMR +RHK
Sbjct: 301 DASLLIYEKKIVSGSFSDLYKGTFYGQEVAIKLLKDGSLNEAVRREFAQEIHIMRNLRHK 360

Query: 361 NVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQK 420
           NVVQFIGA TRPPS  IVTEYM GGSMHDF HQ+KGVLSFPSLLRVAVDVSKGM+YLHQK
Sbjct: 361 NVVQFIGASTRPPSAFIVTEYMPGGSMHDFLHQKKGVLSFPSLLRVAVDVSKGMNYLHQK 420

Query: 421 NIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHK 480
           NIIHRDLKAANLL+DEDGVVKVADFGVARVQ+QSGVMTAETGTYRWMAPEVIEHKPYDHK
Sbjct: 421 NIIHRDLKAANLLLDEDGVVKVADFGVARVQSQSGVMTAETGTYRWMAPEVIEHKPYDHK 480

Query: 481 ADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQD 540
           ADVYSFGIVLWELLTG+LPYDNLTPLQAA+GV+QKGLRPK PRHA+PMIVDLL++CW+QD
Sbjct: 481 ADVYSFGIVLWELLTGQLPYDNLTPLQAAMGVIQKGLRPKIPRHANPMIVDLLERCWRQD 540

Query: 541 PSLRPEFSEITKLLQQTLKEV 550
           PSLRPEFSEITKLLQQTLKE+
Sbjct: 541 PSLRPEFSEITKLLQQTLKEI 543

BLAST of MS021822 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 7.5e-167
Identity = 317/570 (55.61%), Postives = 399/570 (70.00%), Query Frame = 0

Query: 1   MAMDDTESCSSRAV-----DGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDEL 60
           M M+D ESC+SR +        +T   R++ K++V+DEVLRRLR S   +  +PGFED+L
Sbjct: 1   MVMEDNESCASRVIFDALPTSQATMDRRERIKMEVFDEVLRRLRQSDIEDAHLPGFEDDL 60

Query: 61  WAHFHRLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ--------NMMV 120
           W HF+RLP RYA+DVNVERAEDVLMHK LL  A+DP  RPAI+V LVQ        ++  
Sbjct: 61  WNHFNRLPARYALDVNVERAEDVLMHKRLLHSAYDPQNRPAIEVHLVQVQPAGISADLDS 120

Query: 121 SLHSVDQFSPSKQNI---PFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHF 180
           + +     SP++++I   P F      E   +  S         +  ++    SV   H 
Sbjct: 121 TSNDAGHSSPTRKSIHPPPAFGSSPNLEALALAAS---------LSQDEDADNSV---HN 180

Query: 181 NTF--RPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAI 240
           N+   RP+HEIT ST DKPKLL QLT+LL+E+ LNIQEAHAFSTTDG+SLDVFVVDGW  
Sbjct: 181 NSLYSRPLHEITFSTEDKPKLLFQLTALLAELGLNIQEAHAFSTTDGYSLDVFVVDGWPY 240

Query: 241 EVILLLFLIFYFSRFHI-TSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKD 300
           E    L +        I   ++ W  ++       S S   +  QTG +     V IP D
Sbjct: 241 EETERLRISLEKEAAKIELQSQSWPMQQ-------SFSPEKENGQTGAR---THVPIPND 300

Query: 301 EVDVWEIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHI 360
             DVWEI+   L +  KI SGS+ DLYKGT+  Q+VAIK+LK E L+  + +EFAQE+ I
Sbjct: 301 GTDVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFI 360

Query: 361 MRKIRHKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKG 420
           MRK+RHKNVVQFIGACT+PP LCIVTE+M GGS++D+ H+QKGV   P+L +VA+D+ KG
Sbjct: 361 MRKVRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKG 420

Query: 421 MDYLHQKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIE 480
           M YLHQ NIIHRDLKAANLLMDE+ VVKVADFGVARV+AQ+GVMTAETGTYRWMAPEVIE
Sbjct: 421 MSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAETGTYRWMAPEVIE 480

Query: 481 HKPYDHKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLL 540
           HKPYDHKADV+S+GIVLWELLTG+LPY+ +TPLQAA+GVVQKGLRP  P++ HP + +LL
Sbjct: 481 HKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAELL 540

Query: 541 KKCWQQDPSLRPEFSEITKLLQQTLKEVGE 552
           ++ W+ D + RP+FSEI + LQ+  KEVGE
Sbjct: 541 ERLWEHDSTQRPDFSEIIEQLQEIAKEVGE 548

BLAST of MS021822 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.2e-164
Identity = 308/551 (55.90%), Postives = 383/551 (69.51%), Query Frame = 0

Query: 7   ESCSSRAVDGFSTR-SWRQ-KQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFHRLPT 66
           ESC SRAV    T+ S RQ + K++VY EVL+R++ S+  E + PGF+D LW HF+RLP 
Sbjct: 9   ESCGSRAVVASITKESPRQHRMKLEVYGEVLQRIQESNYEEANFPGFDDLLWLHFNRLPA 68

Query: 67  RYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ---NMMVSLHSVDQFSPSKQN 126
           RYA+DVNVERAEDVL H+ LL +A DPA RP  +VR VQ    +  +   VD   P+   
Sbjct: 69  RYALDVNVERAEDVLTHQRLLKLAEDPATRPVFEVRCVQVSPTLNGNSGDVDPSDPAVNE 128

Query: 127 IPFFTLFWQREKKLILLSGIIYYGCGLVYNEKF-IYASVLKEHFNTFRPMHEITISTIDK 186
               +   +        S   +      Y +      S +       RPMHEIT STID+
Sbjct: 129 DAQSSYNSRSLAPPTFGSSPNFEALTQAYKDHAQDDDSAVNAQLPNSRPMHEITFSTIDR 188

Query: 187 PKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILLLFLIFYFSRFHIT 246
           PKLLSQLTS+L E+ LNIQEAHAFST DGFSLDVFVVDGW+ E    L            
Sbjct: 189 PKLLSQLTSMLGELGLNIQEAHAFSTADGFSLDVFVVDGWSQEETEGL--------KDAL 248

Query: 247 STRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEIDASLLIYEKKIV 306
              + + K  P     S++   + D++  + +  CV IP D  D WEID   L  EKK+ 
Sbjct: 249 KKEIRKFKDQPCSKQKSITFF-EHDKSTNELLPACVEIPTDGTDEWEIDMKQLKIEKKVA 308

Query: 307 SGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRHKNVVQFIGACTRP 366
            GS+ +L++GT+  Q+VAIK+LK E +N  + REF+QE++IMRK+RHKNVVQFIGACTR 
Sbjct: 309 CGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRKVRHKNVVQFIGACTRS 368

Query: 367 PSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQKNIIHRDLKAANL 426
           P+LCIVTE+M+ GS++DF H+ KGV    SLL+VA+DVSKGM+YLHQ NIIHRDLK ANL
Sbjct: 369 PNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMNYLHQNNIIHRDLKTANL 428

Query: 427 LMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHKADVYSFGIVLWE 486
           LMDE  VVKVADFGVARVQ +SGVMTAETGTYRWMAPEVIEHKPYDH+ADV+S+ IVLWE
Sbjct: 429 LMDEHEVVKVADFGVARVQTESGVMTAETGTYRWMAPEVIEHKPYDHRADVFSYAIVLWE 488

Query: 487 LLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQDPSLRPEFSEITK 546
           LLTG LPY  LTPLQAA+GVVQKGLRPK P+  HP + +LL+KCWQQDP+LRP F+EI +
Sbjct: 489 LLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLTELLEKCWQQDPALRPNFAEIIE 548

Query: 547 LLQQTLKEVGE 552
           +L Q ++EVG+
Sbjct: 549 MLNQLIREVGD 550

BLAST of MS021822 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 572.8 bits (1475), Expect = 4.2e-162
Identity = 306/557 (54.94%), Postives = 379/557 (68.04%), Query Frame = 0

Query: 4   DDTESCSSRAVDGFSTRS--WRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFHR 63
           D++ESC SRAV    ++      + K+DVY EVL+RL+ S+  E ++P FED+LW HF+R
Sbjct: 5   DESESCGSRAVVASPSQENPRHYRMKLDVYSEVLQRLQESNYEEATLPDFEDQLWLHFNR 64

Query: 64  LPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ---------NMMVSLHSV 123
           LP RYA+DV VERAEDVL H+ LL +A DPA RP  +VR VQ         +  V   + 
Sbjct: 65  LPARYALDVKVERAEDVLTHQRLLKLAADPATRPVFEVRSVQVSPRISADSDPAVEEDAQ 124

Query: 124 DQFSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTFRPMHE 183
               PS   +     F             I  G  +V +   +  + L     + RPMHE
Sbjct: 125 SSHQPSGPGVLAPPTFGSSPN-----FEAITQGSKIVEDVDSVVNATL-----STRPMHE 184

Query: 184 ITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILLLFLIF 243
           IT STIDKPKLLSQLTSLL E+ LNIQEAHAFST DGFSLDVFVVDGW+ E    L    
Sbjct: 185 ITFSTIDKPKLLSQLTSLLGELGLNIQEAHAFSTVDGFSLDVFVVDGWSQEETDGL---- 244

Query: 244 YFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEIDASL 303
                   S  + + K  P     S+S   + D++  + I  C+ IP D  D WEID + 
Sbjct: 245 ----RDALSKEILKLKDQPGSKQKSISFF-EHDKSSNELIPACIEIPTDGTDEWEIDVTQ 304

Query: 304 LIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRHKNVVQ 363
           L  EKK+ SGS+ DL++GT+  Q+VAIK LK + +N  + REF+QE+ IMRK+RHKNVVQ
Sbjct: 305 LKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRKVRHKNVVQ 364

Query: 364 FIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQKNIIH 423
           F+GACTR P+LCIVTE+M+ GS++DF H+QK      +LL+VA+DV+KGM YLHQ NIIH
Sbjct: 365 FLGACTRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQTLLKVALDVAKGMSYLHQNNIIH 424

Query: 424 RDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHKADVY 483
           RDLK ANLLMDE G+VKVADFGVARVQ +SGVMTAETGTYRWMAPEVIEHKPY+HKADV+
Sbjct: 425 RDLKTANLLMDEHGLVKVADFGVARVQIESGVMTAETGTYRWMAPEVIEHKPYNHKADVF 484

Query: 484 SFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQDPSLR 543
           S+ IVLWELLTG +PY  LTPLQAA+GVVQKGLRPK P+  HP +  LL++CW QDP  R
Sbjct: 485 SYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQR 542

Query: 544 PEFSEITKLLQQTLKEV 550
           P F EI ++LQQ +KEV
Sbjct: 545 PLFEEIIEMLQQIMKEV 542

BLAST of MS021822 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 4.1e-72
Identity = 129/271 (47.60%), Postives = 182/271 (67.16%), Query Frame = 0

Query: 284 DVWEIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLL-KDENLNET---VRREFAQEI 343
           D W ID   L        G+F  LYKGT+ G+DVAIK+L + EN  E    + ++F QE+
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPENSPEKAQFMEQQFQQEV 180

Query: 344 HIMRKIRHKNVVQFIGACTRPPSLCIVTEYMSGGSMHDF-CHQQKGVLSFPSLLRVAVDV 403
            ++  ++H N+V+FIGAC +P   CIVTEY  GGS+  F   +Q   +     ++ A+DV
Sbjct: 181 SMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQALDV 240

Query: 404 SKGMDYLHQKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPE 463
           ++GM Y+H +N IHRDLK+ NLL+  D  +K+ADFGVAR++ Q+  MT ETGTYRWMAPE
Sbjct: 241 ARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMAPE 300

Query: 464 VIEHKPYDHKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIV 523
           +I+H+ Y+ K DVYSFGIVLWEL+TG LP+ N+T +QAA  VV +G+RP  P    P++ 
Sbjct: 301 MIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPVLS 360

Query: 524 DLLKKCWQQDPSLRPEFSEITKLLQQTLKEV 550
           D++ +CW  +P +RP F E+ KLL+    E+
Sbjct: 361 DIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of MS021822 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 8.8e-67
Identity = 123/302 (40.73%), Postives = 187/302 (61.92%), Query Frame = 0

Query: 248 KRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEIDASLLIYEKKIVSGSFSDL 307
           KR  S+  WS+    +  +T            K E + W  D S L    K  SG+ S +
Sbjct: 49  KRFDSMESWSMILESENVETW--------EASKGEREEWTADLSQLFIGNKFASGAHSRI 108

Query: 308 YKGTFYGQDVAIKLLK----DENLNETVRREFAQEIHIMRKIRHKNVVQFIGACTRPPSL 367
           Y+G +  + VA+K+++     E     + ++F  E+ ++ ++ H N+VQFI AC +PP  
Sbjct: 109 YRGIYKQRAVAVKMVRIPTHKEETRAKLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVY 168

Query: 368 CIVTEYMSGGSMHDFCHQQKGV-LSFPSLLRVAVDVSKGMDYLHQKNIIHRDLKAANLLM 427
           CI+TEYMS G++  + ++++   LS  ++LR+A+D+S+GM+YLH + +IHRDLK+ NLL+
Sbjct: 169 CIITEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLL 228

Query: 428 DEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHKADVYSFGIVLWELL 487
           +++  VKVADFG + ++ Q        GTYRWMAPE+I+ KPY  K DVYSFGIVLWEL 
Sbjct: 229 NDEMRVKVADFGTSCLETQCREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELT 288

Query: 488 TGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQDPSLRPEFSEITKLL 545
           T  LP+  +TP+QAA  V +K  RP  P    P +  L+K+CW ++PS RP+FS I  +L
Sbjct: 289 TALLPFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVL 342

BLAST of MS021822 vs. ExPASy TrEMBL
Match: A0A6J1CHG2 (serine/threonine-protein kinase STY8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011442 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.6e-259
Identity = 476/562 (84.70%), Postives = 482/562 (85.77%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH
Sbjct: 86  MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 145

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ       N   S+HS  
Sbjct: 146 RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQVHSSPGGNSGKSVHSNL 205

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR               G   NE        ++   T 
Sbjct: 206 KEKVDQRFSDYASN--------QRHHPPPAFGSSTEVEYGFKANELHD-----RDQSGTL 265

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 266 SWPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 325

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  +      +I        KRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW
Sbjct: 326 KLKDVLAEEISNI-------QKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 385

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EIDASLLIYEKKIVSGSFSDLY+GTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR
Sbjct: 386 EIDASLLIYEKKIVSGSFSDLYRGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 445

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH
Sbjct: 446 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 505

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 506 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 565

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ
Sbjct: 566 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 625

Query: 541 QDPSLRPEFSEITKLLQQTLKE 549
           QDPSLRPEFSEITKLLQQTLKE
Sbjct: 626 QDPSLRPEFSEITKLLQQTLKE 627

BLAST of MS021822 vs. ExPASy TrEMBL
Match: A0A6J1CH18 (serine/threonine-protein kinase STY8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011442 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.6e-259
Identity = 476/562 (84.70%), Postives = 483/562 (85.94%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH
Sbjct: 86  MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 145

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ       N   S+HS  
Sbjct: 146 RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQVHSSPGGNSGKSVHSNL 205

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR               G   NE        ++   T 
Sbjct: 206 KEKVDQRFSDYASN--------QRHHPPPAFGSSTEVEYGFKANELHD-----RDQSGTL 265

Query: 181 R-PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVIL 240
             PMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIE   
Sbjct: 266 SWPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEDTK 325

Query: 241 LLFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 300
            L  +      +I      + KRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW
Sbjct: 326 KLKDVLAEEISNI------QVKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVW 385

Query: 301 EIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 360
           EIDASLLIYEKKIVSGSFSDLY+GTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR
Sbjct: 386 EIDASLLIYEKKIVSGSFSDLYRGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIR 445

Query: 361 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 420
           HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH
Sbjct: 446 HKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLH 505

Query: 421 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 480
           QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD
Sbjct: 506 QKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYD 565

Query: 481 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 540
           HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ
Sbjct: 566 HKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQ 625

Query: 541 QDPSLRPEFSEITKLLQQTLKE 549
           QDPSLRPEFSEITKLLQQTLKE
Sbjct: 626 QDPSLRPEFSEITKLLQQTLKE 628

BLAST of MS021822 vs. ExPASy TrEMBL
Match: A0A6J1FL88 (serine/threonine-protein kinase STY46-like isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC111446265 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 5.1e-227
Identity = 422/562 (75.09%), Postives = 453/562 (80.60%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESC SR VD FST++WRQKQKV VY EVLRRLR+SHEAETS PGFEDELWAHFH
Sbjct: 1   MAMDDTESCCSRPVDDFSTQTWRQKQKVGVYVEVLRRLRLSHEAETSFPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERA+DVLMHK+LLFMAHDPA RPAIDVRLVQ       N    +HS  
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKDLLFMAHDPATRPAIDVRLVQVHSSPGTNSGTYVHSNL 120

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR+              G   ++       + +     
Sbjct: 121 KEKVDQRFSDYDSN--------QRQHPPPAFGSTTEVESGFAISQ----LHDINQPDTLS 180

Query: 181 RPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILL 240
            PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE    
Sbjct: 181 WPMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTKQ 240

Query: 241 LFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWE 300
           L  I       I        KRHP   PWS+S TGK+D+ GVKFI N VNIP+ +V   E
Sbjct: 241 LKDILAEEISKI-------QKRHPLFTPWSISPTGKKDKIGVKFIANYVNIPRHKVGARE 300

Query: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRH 360
           IDASLLIYEKKIVSGSFSDLYKGTFYGQ+VAIKLLKD +LNE VRREFAQEIHIMR +RH
Sbjct: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQEVAIKLLKDGSLNEAVRREFAQEIHIMRNLRH 360

Query: 361 KNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQ 420
           KNVVQFIGA TRPPS  IVTEYM GGSMHDF HQ+KGVLSFPSLLRVAVDVSKGM+YLHQ
Sbjct: 361 KNVVQFIGASTRPPSAFIVTEYMPGGSMHDFLHQKKGVLSFPSLLRVAVDVSKGMNYLHQ 420

Query: 421 KNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDH 480
           KNIIHRDLKAANLL+DEDGVVKVADFGVARVQ+QSGVMTAETGTYRWMAPEVIEHKPYDH
Sbjct: 421 KNIIHRDLKAANLLLDEDGVVKVADFGVARVQSQSGVMTAETGTYRWMAPEVIEHKPYDH 480

Query: 481 KADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQ 540
           KADVYSFGIVLWELLTG+LPYDNLTPLQAA+GV+QKGLRPK PRHA+PMIVDLL++CW+Q
Sbjct: 481 KADVYSFGIVLWELLTGQLPYDNLTPLQAAMGVIQKGLRPKIPRHANPMIVDLLERCWRQ 540

Query: 541 DPSLRPEFSEITKLLQQTLKEV 550
           DPSLRPEFSEITKLLQQTLKE+
Sbjct: 541 DPSLRPEFSEITKLLQQTLKEI 543

BLAST of MS021822 vs. ExPASy TrEMBL
Match: A0A6J1FRI5 (serine/threonine-protein kinase STY8-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111446265 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.1e-226
Identity = 422/561 (75.22%), Postives = 452/561 (80.57%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESC SR VD FST++WRQKQKV VY EVLRRLR+SHEAETS PGFEDELWAHFH
Sbjct: 1   MAMDDTESCCSRPVDDFSTQTWRQKQKVGVYVEVLRRLRLSHEAETSFPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERA+DVLMHK+LLFMAHDPA RPAIDVRLVQ       N    +HS  
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKDLLFMAHDPATRPAIDVRLVQVHSSPGTNSGTYVHSNL 120

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR+              G   ++       + +     
Sbjct: 121 KEKVDQRFSDYDSN--------QRQHPPPAFGSTTEVESGFAISQ----LHDINQPDTLS 180

Query: 181 RPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILL 240
            PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE    
Sbjct: 181 WPMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTKQ 240

Query: 241 LFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWE 300
           L  I       I        KRHP   PWS+S TGK+D+ GVKFI N VNIP+ +V   E
Sbjct: 241 LKDILAEEISKI-------QKRHPLFTPWSISPTGKKDKIGVKFIANYVNIPRHKVGARE 300

Query: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRH 360
           IDASLLIYEKKIVSGSFSDLYKGTFYGQ+VAIKLLKD +LNE VRREFAQEIHIMR +RH
Sbjct: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQEVAIKLLKDGSLNEAVRREFAQEIHIMRNLRH 360

Query: 361 KNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQ 420
           KNVVQFIGA TRPPS  IVTEYM GGSMHDF HQ+KGVLSFPSLLRVAVDVSKGM+YLHQ
Sbjct: 361 KNVVQFIGASTRPPSAFIVTEYMPGGSMHDFLHQKKGVLSFPSLLRVAVDVSKGMNYLHQ 420

Query: 421 KNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDH 480
           KNIIHRDLKAANLL+DEDGVVKVADFGVARVQ+QSGVMTAETGTYRWMAPEVIEHKPYDH
Sbjct: 421 KNIIHRDLKAANLLLDEDGVVKVADFGVARVQSQSGVMTAETGTYRWMAPEVIEHKPYDH 480

Query: 481 KADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQ 540
           KADVYSFGIVLWELLTG+LPYDNLTPLQAA+GV+QKGLRPK PRHA+PMIVDLL++CW+Q
Sbjct: 481 KADVYSFGIVLWELLTGQLPYDNLTPLQAAMGVIQKGLRPKIPRHANPMIVDLLERCWRQ 540

Query: 541 DPSLRPEFSEITKLLQQTLKE 549
           DPSLRPEFSEITKLLQQTLKE
Sbjct: 541 DPSLRPEFSEITKLLQQTLKE 542

BLAST of MS021822 vs. ExPASy TrEMBL
Match: A0A6J1FL83 (serine/threonine-protein kinase STY8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446265 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.1e-226
Identity = 422/561 (75.22%), Postives = 452/561 (80.57%), Query Frame = 0

Query: 1   MAMDDTESCSSRAVDGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFH 60
           MAMDDTESC SR VD FST++WRQKQKV VY EVLRRLR+SHEAETS PGFEDELWAHFH
Sbjct: 1   MAMDDTESCCSRPVDDFSTQTWRQKQKVGVYVEVLRRLRLSHEAETSFPGFEDELWAHFH 60

Query: 61  RLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ-------NMMVSLHS-- 120
           RLPTRYAMDVNVERA+DVLMHK+LLFMAHDPA RPAIDVRLVQ       N    +HS  
Sbjct: 61  RLPTRYAMDVNVERAQDVLMHKDLLFMAHDPATRPAIDVRLVQVHSSPGTNSGTYVHSNL 120

Query: 121 ---VDQ-FSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTF 180
              VDQ FS    N        QR+              G   ++       + +     
Sbjct: 121 KEKVDQRFSDYDSN--------QRQHPPPAFGSTTEVESGFAISQ----LHDINQPDTLS 180

Query: 181 RPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILL 240
            PMHEITISTIDKPKLLSQLTSLLSEI LNIQEAHAFSTTDGFSLDVFVVDGWAIE    
Sbjct: 181 WPMHEITISTIDKPKLLSQLTSLLSEIGLNIQEAHAFSTTDGFSLDVFVVDGWAIEDTKQ 240

Query: 241 LFLIFYFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWE 300
           L  I       I        KRHP   PWS+S TGK+D+ GVKFI N VNIP+ +V   E
Sbjct: 241 LKDILAEEISKI-------QKRHPLFTPWSISPTGKKDKIGVKFIANYVNIPRHKVGARE 300

Query: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRH 360
           IDASLLIYEKKIVSGSFSDLYKGTFYGQ+VAIKLLKD +LNE VRREFAQEIHIMR +RH
Sbjct: 301 IDASLLIYEKKIVSGSFSDLYKGTFYGQEVAIKLLKDGSLNEAVRREFAQEIHIMRNLRH 360

Query: 361 KNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQ 420
           KNVVQFIGA TRPPS  IVTEYM GGSMHDF HQ+KGVLSFPSLLRVAVDVSKGM+YLHQ
Sbjct: 361 KNVVQFIGASTRPPSAFIVTEYMPGGSMHDFLHQKKGVLSFPSLLRVAVDVSKGMNYLHQ 420

Query: 421 KNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDH 480
           KNIIHRDLKAANLL+DEDGVVKVADFGVARVQ+QSGVMTAETGTYRWMAPEVIEHKPYDH
Sbjct: 421 KNIIHRDLKAANLLLDEDGVVKVADFGVARVQSQSGVMTAETGTYRWMAPEVIEHKPYDH 480

Query: 481 KADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQ 540
           KADVYSFGIVLWELLTG+LPYDNLTPLQAA+GV+QKGLRPK PRHA+PMIVDLL++CW+Q
Sbjct: 481 KADVYSFGIVLWELLTGQLPYDNLTPLQAAMGVIQKGLRPKIPRHANPMIVDLLERCWRQ 540

Query: 541 DPSLRPEFSEITKLLQQTLKE 549
           DPSLRPEFSEITKLLQQTLKE
Sbjct: 541 DPSLRPEFSEITKLLQQTLKE 542

BLAST of MS021822 vs. TAIR 10
Match: AT4G38470.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 588.6 bits (1516), Expect = 5.3e-168
Identity = 317/570 (55.61%), Postives = 399/570 (70.00%), Query Frame = 0

Query: 1   MAMDDTESCSSRAV-----DGFSTRSWRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDEL 60
           M M+D ESC+SR +        +T   R++ K++V+DEVLRRLR S   +  +PGFED+L
Sbjct: 1   MVMEDNESCASRVIFDALPTSQATMDRRERIKMEVFDEVLRRLRQSDIEDAHLPGFEDDL 60

Query: 61  WAHFHRLPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ--------NMMV 120
           W HF+RLP RYA+DVNVERAEDVLMHK LL  A+DP  RPAI+V LVQ        ++  
Sbjct: 61  WNHFNRLPARYALDVNVERAEDVLMHKRLLHSAYDPQNRPAIEVHLVQVQPAGISADLDS 120

Query: 121 SLHSVDQFSPSKQNI---PFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHF 180
           + +     SP++++I   P F      E   +  S         +  ++    SV   H 
Sbjct: 121 TSNDAGHSSPTRKSIHPPPAFGSSPNLEALALAAS---------LSQDEDADNSV---HN 180

Query: 181 NTF--RPMHEITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAI 240
           N+   RP+HEIT ST DKPKLL QLT+LL+E+ LNIQEAHAFSTTDG+SLDVFVVDGW  
Sbjct: 181 NSLYSRPLHEITFSTEDKPKLLFQLTALLAELGLNIQEAHAFSTTDGYSLDVFVVDGWPY 240

Query: 241 EVILLLFLIFYFSRFHI-TSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKD 300
           E    L +        I   ++ W  ++       S S   +  QTG +     V IP D
Sbjct: 241 EETERLRISLEKEAAKIELQSQSWPMQQ-------SFSPEKENGQTGAR---THVPIPND 300

Query: 301 EVDVWEIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHI 360
             DVWEI+   L +  KI SGS+ DLYKGT+  Q+VAIK+LK E L+  + +EFAQE+ I
Sbjct: 301 GTDVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFI 360

Query: 361 MRKIRHKNVVQFIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKG 420
           MRK+RHKNVVQFIGACT+PP LCIVTE+M GGS++D+ H+QKGV   P+L +VA+D+ KG
Sbjct: 361 MRKVRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKG 420

Query: 421 MDYLHQKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIE 480
           M YLHQ NIIHRDLKAANLLMDE+ VVKVADFGVARV+AQ+GVMTAETGTYRWMAPEVIE
Sbjct: 421 MSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAETGTYRWMAPEVIE 480

Query: 481 HKPYDHKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLL 540
           HKPYDHKADV+S+GIVLWELLTG+LPY+ +TPLQAA+GVVQKGLRP  P++ HP + +LL
Sbjct: 481 HKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAELL 540

Query: 541 KKCWQQDPSLRPEFSEITKLLQQTLKEVGE 552
           ++ W+ D + RP+FSEI + LQ+  KEVGE
Sbjct: 541 ERLWEHDSTQRPDFSEIIEQLQEIAKEVGE 548

BLAST of MS021822 vs. TAIR 10
Match: AT4G35780.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 581.3 bits (1497), Expect = 8.5e-166
Identity = 308/551 (55.90%), Postives = 383/551 (69.51%), Query Frame = 0

Query: 7   ESCSSRAVDGFSTR-SWRQ-KQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFHRLPT 66
           ESC SRAV    T+ S RQ + K++VY EVL+R++ S+  E + PGF+D LW HF+RLP 
Sbjct: 9   ESCGSRAVVASITKESPRQHRMKLEVYGEVLQRIQESNYEEANFPGFDDLLWLHFNRLPA 68

Query: 67  RYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ---NMMVSLHSVDQFSPSKQN 126
           RYA+DVNVERAEDVL H+ LL +A DPA RP  +VR VQ    +  +   VD   P+   
Sbjct: 69  RYALDVNVERAEDVLTHQRLLKLAEDPATRPVFEVRCVQVSPTLNGNSGDVDPSDPAVNE 128

Query: 127 IPFFTLFWQREKKLILLSGIIYYGCGLVYNEKF-IYASVLKEHFNTFRPMHEITISTIDK 186
               +   +        S   +      Y +      S +       RPMHEIT STID+
Sbjct: 129 DAQSSYNSRSLAPPTFGSSPNFEALTQAYKDHAQDDDSAVNAQLPNSRPMHEITFSTIDR 188

Query: 187 PKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILLLFLIFYFSRFHIT 246
           PKLLSQLTS+L E+ LNIQEAHAFST DGFSLDVFVVDGW+ E    L            
Sbjct: 189 PKLLSQLTSMLGELGLNIQEAHAFSTADGFSLDVFVVDGWSQEETEGL--------KDAL 248

Query: 247 STRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEIDASLLIYEKKIV 306
              + + K  P     S++   + D++  + +  CV IP D  D WEID   L  EKK+ 
Sbjct: 249 KKEIRKFKDQPCSKQKSITFF-EHDKSTNELLPACVEIPTDGTDEWEIDMKQLKIEKKVA 308

Query: 307 SGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRHKNVVQFIGACTRP 366
            GS+ +L++GT+  Q+VAIK+LK E +N  + REF+QE++IMRK+RHKNVVQFIGACTR 
Sbjct: 309 CGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRKVRHKNVVQFIGACTRS 368

Query: 367 PSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQKNIIHRDLKAANL 426
           P+LCIVTE+M+ GS++DF H+ KGV    SLL+VA+DVSKGM+YLHQ NIIHRDLK ANL
Sbjct: 369 PNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMNYLHQNNIIHRDLKTANL 428

Query: 427 LMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHKADVYSFGIVLWE 486
           LMDE  VVKVADFGVARVQ +SGVMTAETGTYRWMAPEVIEHKPYDH+ADV+S+ IVLWE
Sbjct: 429 LMDEHEVVKVADFGVARVQTESGVMTAETGTYRWMAPEVIEHKPYDHRADVFSYAIVLWE 488

Query: 487 LLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQDPSLRPEFSEITK 546
           LLTG LPY  LTPLQAA+GVVQKGLRPK P+  HP + +LL+KCWQQDP+LRP F+EI +
Sbjct: 489 LLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLTELLEKCWQQDPALRPNFAEIIE 548

Query: 547 LLQQTLKEVGE 552
           +L Q ++EVG+
Sbjct: 549 MLNQLIREVGD 550

BLAST of MS021822 vs. TAIR 10
Match: AT2G17700.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 572.8 bits (1475), Expect = 3.0e-163
Identity = 306/557 (54.94%), Postives = 379/557 (68.04%), Query Frame = 0

Query: 4   DDTESCSSRAVDGFSTRS--WRQKQKVDVYDEVLRRLRVSHEAETSVPGFEDELWAHFHR 63
           D++ESC SRAV    ++      + K+DVY EVL+RL+ S+  E ++P FED+LW HF+R
Sbjct: 5   DESESCGSRAVVASPSQENPRHYRMKLDVYSEVLQRLQESNYEEATLPDFEDQLWLHFNR 64

Query: 64  LPTRYAMDVNVERAEDVLMHKNLLFMAHDPAARPAIDVRLVQ---------NMMVSLHSV 123
           LP RYA+DV VERAEDVL H+ LL +A DPA RP  +VR VQ         +  V   + 
Sbjct: 65  LPARYALDVKVERAEDVLTHQRLLKLAADPATRPVFEVRSVQVSPRISADSDPAVEEDAQ 124

Query: 124 DQFSPSKQNIPFFTLFWQREKKLILLSGIIYYGCGLVYNEKFIYASVLKEHFNTFRPMHE 183
               PS   +     F             I  G  +V +   +  + L     + RPMHE
Sbjct: 125 SSHQPSGPGVLAPPTFGSSPN-----FEAITQGSKIVEDVDSVVNATL-----STRPMHE 184

Query: 184 ITISTIDKPKLLSQLTSLLSEIELNIQEAHAFSTTDGFSLDVFVVDGWAIEVILLLFLIF 243
           IT STIDKPKLLSQLTSLL E+ LNIQEAHAFST DGFSLDVFVVDGW+ E    L    
Sbjct: 185 ITFSTIDKPKLLSQLTSLLGELGLNIQEAHAFSTVDGFSLDVFVVDGWSQEETDGL---- 244

Query: 244 YFSRFHITSTRLWRDKRHPSVAPWSVSHTGKQDQTGVKFITNCVNIPKDEVDVWEIDASL 303
                   S  + + K  P     S+S   + D++  + I  C+ IP D  D WEID + 
Sbjct: 245 ----RDALSKEILKLKDQPGSKQKSISFF-EHDKSSNELIPACIEIPTDGTDEWEIDVTQ 304

Query: 304 LIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLNETVRREFAQEIHIMRKIRHKNVVQ 363
           L  EKK+ SGS+ DL++GT+  Q+VAIK LK + +N  + REF+QE+ IMRK+RHKNVVQ
Sbjct: 305 LKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRKVRHKNVVQ 364

Query: 364 FIGACTRPPSLCIVTEYMSGGSMHDFCHQQKGVLSFPSLLRVAVDVSKGMDYLHQKNIIH 423
           F+GACTR P+LCIVTE+M+ GS++DF H+QK      +LL+VA+DV+KGM YLHQ NIIH
Sbjct: 365 FLGACTRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQTLLKVALDVAKGMSYLHQNNIIH 424

Query: 424 RDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVIEHKPYDHKADVY 483
           RDLK ANLLMDE G+VKVADFGVARVQ +SGVMTAETGTYRWMAPEVIEHKPY+HKADV+
Sbjct: 425 RDLKTANLLMDEHGLVKVADFGVARVQIESGVMTAETGTYRWMAPEVIEHKPYNHKADVF 484

Query: 484 SFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDLLKKCWQQDPSLR 543
           S+ IVLWELLTG +PY  LTPLQAA+GVVQKGLRPK P+  HP +  LL++CW QDP  R
Sbjct: 485 SYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQR 542

Query: 544 PEFSEITKLLQQTLKEV 550
           P F EI ++LQQ +KEV
Sbjct: 545 PLFEEIIEMLQQIMKEV 542

BLAST of MS021822 vs. TAIR 10
Match: AT4G31170.1 (Protein kinase superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.2e-73
Identity = 127/269 (47.21%), Postives = 181/269 (67.29%), Query Frame = 0

Query: 286 WEIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLN----ETVRREFAQEIHI 345
           W ID   L        G+F  LY+GT+ G+DVAIKLL+  + N    + + ++F QE+ +
Sbjct: 124 WTIDLRKLHMGPAFAQGAFGKLYRGTYNGEDVAIKLLERSDSNPEKAQALEQQFQQEVSM 183

Query: 346 MRKIRHKNVVQFIGACTRPPSLCIVTEYMSGGSMHDF-CHQQKGVLSFPSLLRVAVDVSK 405
           +  ++H N+V+FIGAC +P   CIVTEY  GGS+  F   +Q   +     +  A+DV++
Sbjct: 184 LAFLKHPNIVRFIGACIKPMVWCIVTEYAKGGSVRQFLTKRQNRAVPLKLAVMQALDVAR 243

Query: 406 GMDYLHQKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVI 465
           GM Y+H++N IHRDLK+ NLL+  D  +K+ADFGVAR++ Q+  MT ETGTYRWMAPE+I
Sbjct: 244 GMAYVHERNFIHRDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMI 303

Query: 466 EHKPYDHKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDL 525
           +H+PY  K DVYSFGIVLWEL+TG LP+ N+T +QAA  VV +G+RP  P    P++ ++
Sbjct: 304 QHRPYTQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPADCLPVLGEI 363

Query: 526 LKKCWQQDPSLRPEFSEITKLLQQTLKEV 550
           + +CW  DP +RP F+EI  LL+    E+
Sbjct: 364 MTRCWDADPEVRPCFAEIVNLLEAAETEI 392

BLAST of MS021822 vs. TAIR 10
Match: AT4G31170.2 (Protein kinase superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.2e-73
Identity = 127/269 (47.21%), Postives = 181/269 (67.29%), Query Frame = 0

Query: 286 WEIDASLLIYEKKIVSGSFSDLYKGTFYGQDVAIKLLKDENLN----ETVRREFAQEIHI 345
           W ID   L        G+F  LY+GT+ G+DVAIKLL+  + N    + + ++F QE+ +
Sbjct: 124 WTIDLRKLHMGPAFAQGAFGKLYRGTYNGEDVAIKLLERSDSNPEKAQALEQQFQQEVSM 183

Query: 346 MRKIRHKNVVQFIGACTRPPSLCIVTEYMSGGSMHDF-CHQQKGVLSFPSLLRVAVDVSK 405
           +  ++H N+V+FIGAC +P   CIVTEY  GGS+  F   +Q   +     +  A+DV++
Sbjct: 184 LAFLKHPNIVRFIGACIKPMVWCIVTEYAKGGSVRQFLTKRQNRAVPLKLAVMQALDVAR 243

Query: 406 GMDYLHQKNIIHRDLKAANLLMDEDGVVKVADFGVARVQAQSGVMTAETGTYRWMAPEVI 465
           GM Y+H++N IHRDLK+ NLL+  D  +K+ADFGVAR++ Q+  MT ETGTYRWMAPE+I
Sbjct: 244 GMAYVHERNFIHRDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMI 303

Query: 466 EHKPYDHKADVYSFGIVLWELLTGRLPYDNLTPLQAAIGVVQKGLRPKTPRHAHPMIVDL 525
           +H+PY  K DVYSFGIVLWEL+TG LP+ N+T +QAA  VV +G+RP  P    P++ ++
Sbjct: 304 QHRPYTQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPADCLPVLGEI 363

Query: 526 LKKCWQQDPSLRPEFSEITKLLQQTLKEV 550
           + +CW  DP +RP F+EI  LL+    E+
Sbjct: 364 MTRCWDADPEVRPCFAEIVNLLEAAETEI 392