MS020762 (gene) Bitter gourd (TR) v1

Overview
NameMS020762
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
Locationscaffold487: 262175 .. 266534 (-)
RNA-Seq ExpressionMS020762
SyntenyMS020762
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAACGATATCTGTTTGCCCATCACATTTCCACCTGATCTTCTAGACGTTACCCGCTATCTTCCCCCTGTCTTTCTCTTCAGTCTTCAATGGAGACGCCATTAATGGACCTTTCTTCCCACAGCCCATTTTTCACCCCCAAGAATCATTCCTTATACCATTTTTACCCTTCAAAATTAACCATTCCTACCCTCTGTTGTCCCAATAACCCCACCACTTCCCGTCGCCGGTTTTGCGCCGATACGACGCCGCCTGAAGACATTCACGCAGCAGCTTCAGATTCGGCCCCTGAAAGAACCCAGAACCGCCGCAGGATAGTCCGATTAGCGTGGGAGAAGTTGGTCCGATGGTCCAGGTCTTGGCGGTTCAAGGCCAGGACCGATGTTCTTGAGCGTACGAACAAGGTGGGTGGGTTTTAATCTGCTCGTTTTCTTTTAACTACTTGTGTCTTTTTATTTTTGCCTGATGAATATGTGAGCTAATCTTATTATTGAGCTTTAGCTGTGTGTAATTCGACACTGCCTGCAACTTGTTTGTTAAAATGCGGCAGAGAAGTTCCACTGTAGCATCTTGTTTTTTCTTCATTTTCTTTCCCAATTTGTAAGAGCAGTATAGGAATTTCTTTTAATGTTCGAAAATATTACCATCACAAATACATGTGAAAGTTCGCAATATTCGAACTTGCAACTCTTAGAGGTAGAGATGTCTTAACCGCTTAAATTGGCTGAATTTTAGGAATTGTTGGACCTTGTTCTCAAATTAATCAGTTGTATTTGCCCTGGAAATTCGTAGAAGTTCAACTTTCTTGTCCCTATAGCTTACCTCATTGTACTCTCTTGTGAGTTGTAATTTTGAACGAGTCCCATTGTATGGCATTGTTTTTCCAAGTACTAAAGATTTACTTCCTCTTTGGGCATGGAGAACGATGACTATTATTGGGGGGAATGATGACCACTAGAAAATTAGTCAACACCATATTACTTGACTTTGAATTTAAGTACCAATAAACTTGATGGCATCTAATTTACTACCACCTTGTCAAAGTTATTTTTCCATTCTTTGCTATATCCGTTATTTGGGTGCGGGGCGTAATTTAAGGTGTGTTGGAAAGTGACTTTTGGCCCTAGAGACTACAAGGTTTGACCGTTTGAGTCTAACATTTTTCTTTGAAATTAATAGTTTATTTGACCATCTAGGATATGAAATAATCAAATATAAAAAGGTGTAATCATATTGGAGCTAATAAAATAGAGCTCTAAAGTAGCTTAACTGTGTAGGTATTAATGATATTTTATTGGTAAACGTGGTTGTCCCACCTTGCTAAGAGTCAAGAGACAAGCCTTCAAAATTAGTACACTAGTTATAGTATCTCAAACCCAATTAATTATCAGCAAGACCTTTTGCATCCTTGCAAGTGCTTGGGTGGGCATCACAGTGTATAAAGGTTGAATTTGGCACTTTAGGACGCTTTTGCACTAAATATCAATATCCTTGTGAAAAAAAATCAACAATTCTCAAAGTAATATCTCTTGTTATTAAAGAAGTTATCCTCATATAATATTTCTACTCTAAGTCAGGCAGCAAACATTTCTCTGAACTCTACTGTTTCTTTTATTCCAGTGATCAATAATTGTGTCATTAATATTTTATTCGTCACCAAGGTCAACATCACTATGCTTAGCTTAAATGTCAAACTTCTAGTGCAATAGACTGAGTCTTTTTCAAAATGATATTTGTTTAAAGCATATTCTACTTCCAAAATTTCTTTTTTCTTATTTAATAGTTCATTGTGTCAATTTTATCAGGTGGCTGTGCTTGGAGGGGGATCTTTTGGTACAGCAATGGCTGCCCATATTGCTGGTAGAAAGCCCCAATTGGAAGTCAACATGCTTCTTCGTGATCCTCAAGTATGCCAGTCAATTAATGATACCCACTACAATTGGTAAATTTCCATGTACTCCAAAGAACCTTTCCTTTCCTCCACTCTTTTGTGATGTATGTGAACTGGTGCATGTTTGCGTGGTCTCAGATGAAAGTTTGCCTGATTGAATATGATTGATGACATTTCTTCTCATTCTCAGTAAGTACTTCCCAACGCACAAGCTACCAGTAAATGTTATTGCTACAACTGATGCCAAAGCTGCTTTGCTAGGTGCTGATTTCTGTTTTCACGCTGTACCTGTGCAGGTCTTTCGCTACCCTGTTTAAATTTGTGCATTAATTTTTGTTTGCTCTTGTAGCTATGAAATAATTACGAAGATGTAATGGGGAAAAAACATAGAATGCAGTTCCTTCTTTACAAATTCTTGCAAACAAATTCAAAATTTTTTCATATGCATTTTTAGGCCATTAAGAAATGTGGATCTTCATCATGTTCTCTTTTCTGGGGGATTAGAGTATTTTTTAAAATTTCATTGCAGTTCAGCTCATCGTTTCTTGAGAGCATTGCTGATCATGTTGATCCACACGTGCCATTCATATCCCTAAGCAAAGGGCTGGAACTTAATACCTTGAGGATGATGTCTCAGATTATACCACAAGCACTCAGTAACCCTCGACAACCTTTTATTGCTCTTTCAGGACCTTCATTTGCTTCAGAAATAATGAATAAATTGCCAACAGGTGATAACACACATTATATACATATTCCTTAGTACACACTCATTTCAGTTGTATTATTTTAAAATTTATTTTGCACTTATATCTTGATTTTCCAGCAATGGTAGTGGCATCAAAGGACAAAAAATTGGCAAATGCAGCCCAACAACTACTAGCTTCTAGTTATCTAAGAATAAGCACTTCAAGGTAGATTTAATTATCATAAAATGTGGGCTTTCTTGTCTTGTATATTGTAATTCAAAAATACCTTCTGGTAGAAACTTTAATTCCTCCTTTTGGTATGATCATCTTATGAAGCATCTAATTTTTGACATTCAGTCATTCATCCACTTTTTTCATTTCTACTTCAATATTTGTTTATTTCTCCATTGCCTAAGCACAAATTCCCTCGTAAATAGTTTCTTTTCAGAAAATAACATAAACCGCAAGGAAAAGATAGCAGTAAACTCTTGAGGAAATGATCTGTAAGAGTAAGCCTGTTTACATGGCTTGTAACTTCTGATGACTCAGTGATGTCACAGGGGTGGAGATTGCAGGTGCACTAAAGAATGTGCTTGCAATAGCAGCAGGAATTGTTGAGGGGATGAATCTTGGTAACAACTCTATGGCAGCTCTTGTAGCACAAGGGTGTTCTGAGATCCGTTGGCTGGCAACAAAGGTGTGCTTTTATTCATCATTAATTTCTTGGAACATGAGTATGATTTATTTACACTTAAATTATCGCTCTTTATCCAATAACTCGAGGAAGCCAATGGAGCTACTTACCAGTTGCCATCAACCATTGAAATATTGATTGATAAAAGTTATTAGAAGCATATTTGTAAAGATCTTTTTTCTTTACATCCGCAAGTGTCCAGGCCAACTTACGCATACCTCAACTAATCTCGAGATAACCGCCCGACCCTACAACATTTGGTGTCGGGAAATACATAGGAATTACTAATTCCTAAGGTAGGTGACCATCATGGGGTTTAAACCCATTCCCTCTTAATTAACCCTAGTTCATTTGGACCAATGAGTCATCCATGATGGTTAGAGTACACTCATGTTGTAAAGATCATTTGGCTAGAGTATGCACATATATCGAAAAGATAATAATAATAATGGAGTCCTTTCTCTTTGCCTGCTAATGTAATCAGATTGTTACAGAGAGCTGACTGCTTTTTATTGCCTTTGGTGTAACTTGCAATTGGATTGTTGGCCTCACATTCCTGCTGTCAGTCATAGTTCAAGCCTTATCTGCATTAAACACATTATTATCTTTCATATCTACAATTTGTCTGAATGCAATCATGGCATTCTGATTACTTTTTCCTTCAAAATTTTTTTCTTAAAGATGGGAGCGAAGCCAACGACAATTACCGGTCTCTCGGGCACAGGGGACATTATGCTTACATGTTTTGTCAACCTCTCCCGAAACCGAACTGTTGGAGTACGACTAGGATCTGGCGAGAATCTGGATGATATATTGAGCTCCATGAATCAGGTACTGGGTTATCCTTTGGGTAGCATGTCAGGCAATGTTTGGTGTCCATTTATAATGCCAGCAGTTGTTAAGGACCCCTGTATTCAATAGGTTGCAGAAGGTGTATCGACTGCAGGGGCTGTAATCGCATTGGCTCAGAAGTATAATGTAAAGATGCCAGTTTTGACAGCGGTGGCCCGGATCATTGACAACGAGCTAACGCCACAAAAAGCGGTTCTTGAATTGATGGGGCTTCCTCAGGTAAAACTCTTA

mRNA sequence

AAAGAAAACGATATCTGTTTGCCCATCACATTTCCACCTGATCTTCTAGACGTTACCCGCTATCTTCCCCCTGTCTTTCTCCAGTCTTCAATGGAGACGCCATTAATGGACCTTTCTTCCCACAGCCCATTTTTCACCCCCAAGAATCATTCCTTATACCATTTTTACCCTTCAAAATTAACCATTCCTACCCTCTGTTGTCCCAATAACCCCACCACTTCCCGTCGCCGGTTTTGCGCCGATACGACGCCGCCTGAAGACATTCACGCAGCAGCTTCAGATTCGGCCCCTGAAAGAACCCAGAACCGCCGCAGGATAGTCCGATTAGCGTGGGAGAAGTTGGTCCGATGGTCCAGGTCTTGGCGGTTCAAGGCCAGGACCGATGTTCTTGAGCGTACGAACAAGGTGGCTGTGCTTGGAGGGGGATCTTTTGGTACAGCAATGGCTGCCCATATTGCTGGTAGAAAGCCCCAATTGGAAGTCAACATGCTTCTTCGTGATCCTCAAGTATGCCAGTCAATTAATGATACCCACTACAATTGTAAGTACTTCCCAACGCACAAGCTACCAGTAAATGTTATTGCTACAACTGATGCCAAAGCTGCTTTGCTAGGTGCTGATTTCTGTTTTCACGCTGTACCTGTGCAGTTCAGCTCATCGTTTCTTGAGAGCATTGCTGATCATGTTGATCCACACGTGCCATTCATATCCCTAAGCAAAGGGCTGGAACTTAATACCTTGAGGATGATGTCTCAGATTATACCACAAGCACTCAGTAACCCTCGACAACCTTTTATTGCTCTTTCAGGACCTTCATTTGCTTCAGAAATAATGAATAAATTGCCAACAGCAATGGTAGTGGCATCAAAGGACAAAAAATTGGCAAATGCAGCCCAACAACTACTAGCTTCTAGTTATCTAAGAATAAGCACTTCAAGTGATGTCACAGGGGTGGAGATTGCAGGTGCACTAAAGAATGTGCTTGCAATAGCAGCAGGAATTGTTGAGGGGATGAATCTTGGTAACAACTCTATGGCAGCTCTTGTAGCACAAGGGTGTTCTGAGATCCGTTGGCTGGCAACAAAGATGGGAGCGAAGCCAACGACAATTACCGGTCTCTCGGGCACAGGGGACATTATGCTTACATGTTTTGTCAACCTCTCCCGAAACCGAACTGTTGGAGTACGACTAGGATCTGGCGAGAATCTGGATGATATATTGAGCTCCATGAATCAGGTTGCAGAAGGTGTATCGACTGCAGGGGCTGTAATCGCATTGGCTCAGAAGTATAATGTAAAGATGCCAGTTTTGACAGCGGTGGCCCGGATCATTGACAACGAGCTAACGCCACAAAAAGCGGTTCTTGAATTGATGGGGCTTCCTCAGGTAAAACTCTTA

Coding sequence (CDS)

AAAGAAAACGATATCTGTTTGCCCATCACATTTCCACCTGATCTTCTAGACGTTACCCGCTATCTTCCCCCTGTCTTTCTCCAGTCTTCAATGGAGACGCCATTAATGGACCTTTCTTCCCACAGCCCATTTTTCACCCCCAAGAATCATTCCTTATACCATTTTTACCCTTCAAAATTAACCATTCCTACCCTCTGTTGTCCCAATAACCCCACCACTTCCCGTCGCCGGTTTTGCGCCGATACGACGCCGCCTGAAGACATTCACGCAGCAGCTTCAGATTCGGCCCCTGAAAGAACCCAGAACCGCCGCAGGATAGTCCGATTAGCGTGGGAGAAGTTGGTCCGATGGTCCAGGTCTTGGCGGTTCAAGGCCAGGACCGATGTTCTTGAGCGTACGAACAAGGTGGCTGTGCTTGGAGGGGGATCTTTTGGTACAGCAATGGCTGCCCATATTGCTGGTAGAAAGCCCCAATTGGAAGTCAACATGCTTCTTCGTGATCCTCAAGTATGCCAGTCAATTAATGATACCCACTACAATTGTAAGTACTTCCCAACGCACAAGCTACCAGTAAATGTTATTGCTACAACTGATGCCAAAGCTGCTTTGCTAGGTGCTGATTTCTGTTTTCACGCTGTACCTGTGCAGTTCAGCTCATCGTTTCTTGAGAGCATTGCTGATCATGTTGATCCACACGTGCCATTCATATCCCTAAGCAAAGGGCTGGAACTTAATACCTTGAGGATGATGTCTCAGATTATACCACAAGCACTCAGTAACCCTCGACAACCTTTTATTGCTCTTTCAGGACCTTCATTTGCTTCAGAAATAATGAATAAATTGCCAACAGCAATGGTAGTGGCATCAAAGGACAAAAAATTGGCAAATGCAGCCCAACAACTACTAGCTTCTAGTTATCTAAGAATAAGCACTTCAAGTGATGTCACAGGGGTGGAGATTGCAGGTGCACTAAAGAATGTGCTTGCAATAGCAGCAGGAATTGTTGAGGGGATGAATCTTGGTAACAACTCTATGGCAGCTCTTGTAGCACAAGGGTGTTCTGAGATCCGTTGGCTGGCAACAAAGATGGGAGCGAAGCCAACGACAATTACCGGTCTCTCGGGCACAGGGGACATTATGCTTACATGTTTTGTCAACCTCTCCCGAAACCGAACTGTTGGAGTACGACTAGGATCTGGCGAGAATCTGGATGATATATTGAGCTCCATGAATCAGGTTGCAGAAGGTGTATCGACTGCAGGGGCTGTAATCGCATTGGCTCAGAAGTATAATGTAAAGATGCCAGTTTTGACAGCGGTGGCCCGGATCATTGACAACGAGCTAACGCCACAAAAAGCGGTTCTTGAATTGATGGGGCTTCCTCAGGTAAAACTCTTA

Protein sequence

KENDICLPITFPPDLLDVTRYLPPVFLQSSMETPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPPEDIHAAASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTPQKAVLELMGLPQVKLL
Homology
BLAST of MS020762 vs. NCBI nr
Match: XP_022146729.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Momordica charantia])

HSP 1 Score: 840.9 bits (2171), Expect = 5.4e-240
Identity = 431/434 (99.31%), Postives = 433/434 (99.77%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPPEDIHA 90
           METPLMDLSSHSPFFTPKNHSLYHFYPSK+TIPTLCCPNNPTTS RRFCADTTPPEDIHA
Sbjct: 1   METPLMDLSSHSPFFTPKNHSLYHFYPSKITIPTLCCPNNPTTSLRRFCADTTPPEDIHA 60

Query: 91  AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA 150
           AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA
Sbjct: 61  AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA 120

Query: 151 HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF 210
           HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF
Sbjct: 121 HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF 180

Query: 211 HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG 270
           HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG
Sbjct: 181 HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG 240

Query: 271 PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI 330
           PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI
Sbjct: 241 PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI 300

Query: 331 AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN 390
           AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN
Sbjct: 301 AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN 360

Query: 391 RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP 450
           RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP
Sbjct: 361 RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP 420

Query: 451 QKAVLELMGLPQVK 465
           QKAVLELMGLPQV+
Sbjct: 421 QKAVLELMGLPQVE 434

BLAST of MS020762 vs. NCBI nr
Match: XP_008456964.1 (PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucumis melo])

HSP 1 Score: 763.8 bits (1971), Expect = 8.4e-217
Identity = 405/464 (87.28%), Postives = 422/464 (90.95%), Query Frame = 0

Query: 11  FPPDLLDVTRYLPPVFL--------QSSMETPLMDLSSHSPFFTPKNHSLYHFYPSKLTI 70
           FPPD LD+TRYL  VFL          SMETP MDL S++PF T  + S  H YPS+LTI
Sbjct: 14  FPPDPLDITRYLLAVFLCDTQLSYNNPSMETPFMDLPSNNPFSTLNHPSFSHTYPSRLTI 73

Query: 71  PTLCCPNNPTTSRRRFCADTTPPEDIH--AAASDSAPERTQNRRRIVRLAWEKLVRWSRS 130
           P+L  PN PTTS  RFCA+TTPP  +    A  DS PERTQNRRR+VR+AWEKLVRWSRS
Sbjct: 74  PSLSSPNYPTTSPLRFCANTTPPPILQDTDALLDSGPERTQNRRRVVRIAWEKLVRWSRS 133

Query: 131 WRFKARTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYN 190
            RFKA+TDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSIND HYN
Sbjct: 134 LRFKAKTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDGHYN 193

Query: 191 CKYFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSK 250
            KYFP HKLP+NVIATTDAKAALLGADFCFHAVPVQFSSSFLESIA HVDPH+PFISLSK
Sbjct: 194 SKYFPKHKLPINVIATTDAKAALLGADFCFHAVPVQFSSSFLESIAHHVDPHLPFISLSK 253

Query: 251 GLELNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQ 310
           GLELN+LRMMSQIIPQALSNPRQPF+ALSGPSFASE+MNKLPTAMVVASKDKKLANAAQQ
Sbjct: 254 GLELNSLRMMSQIIPQALSNPRQPFVALSGPSFASELMNKLPTAMVVASKDKKLANAAQQ 313

Query: 311 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA 370
           LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA
Sbjct: 314 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA 373

Query: 371 TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA 430
           TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA
Sbjct: 374 TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA 433

Query: 431 GAVIALAQKYNVKMPVLTAVARIIDNELTPQKAVLELMGLPQVK 465
           GAVIALAQKY VKMPVLTAVARIIDNELTPQKAVLELMGLPQV+
Sbjct: 434 GAVIALAQKYKVKMPVLTAVARIIDNELTPQKAVLELMGLPQVE 477

BLAST of MS020762 vs. NCBI nr
Match: XP_022995739.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 397/437 (90.85%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPP---ED 90
           METPL+DLSS + FFTPKN+SLY+  PSKLT  +LCCP+ PTTS RRFCAD +PP   ED
Sbjct: 1   METPLLDLSSRNAFFTPKNYSLYYTKPSKLTNLSLCCPDKPTTSHRRFCADESPPPILED 60

Query: 91  IHAAASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTA 150
                 D   E+T NRRRIVRLAWEKLVRWSRSWRFKA+TDVLERTNKVAVLGGGSFGTA
Sbjct: 61  TDVVL-DLVTEKTPNRRRIVRLAWEKLVRWSRSWRFKAKTDVLERTNKVAVLGGGSFGTA 120

Query: 151 MAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGAD 210
           MAAHIAGRKPQLEVNMLLRDPQVCQSIND+HYN KYFP HKLP+NVIATTDAKAALLGAD
Sbjct: 121 MAAHIAGRKPQLEVNMLLRDPQVCQSINDSHYNSKYFPRHKLPINVIATTDAKAALLGAD 180

Query: 211 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIA 270
           FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIP AL NPRQPF+A
Sbjct: 181 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPHALRNPRQPFVA 240

Query: 271 LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 330
           LSGPSFASE+MNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV
Sbjct: 241 LSGPSFASELMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 300

Query: 331 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 390
           LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL
Sbjct: 301 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 360

Query: 391 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 450
           SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE
Sbjct: 361 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 420

Query: 451 LTPQKAVLELMGLPQVK 465
           LTPQKAVLELMGLPQV+
Sbjct: 421 LTPQKAVLELMGLPQVE 436

BLAST of MS020762 vs. NCBI nr
Match: XP_022942269.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucurbita moschata] >KAG6599938.1 Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030617.1 Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 759.2 bits (1959), Expect = 2.1e-215
Identity = 397/437 (90.85%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPP---ED 90
           METPL+DLSS + FFTPKN+SLY+  PSKLT  +LCCP+ PTTS RRFCAD +PP   ED
Sbjct: 1   METPLLDLSSRNAFFTPKNYSLYYTKPSKLTNFSLCCPDKPTTSHRRFCADESPPPILED 60

Query: 91  IHAAASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTA 150
                 D   E+T NRRRIVRLAWEKLVRWSRSWRFKA+TDVLERTNKVAVLGGGSFGTA
Sbjct: 61  TDVVL-DLVTEKTPNRRRIVRLAWEKLVRWSRSWRFKAKTDVLERTNKVAVLGGGSFGTA 120

Query: 151 MAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGAD 210
           MAAHIAGRKPQLEVNMLLRDPQVCQSIND+HYN KYFP HKLP+NVIATTDAKAALLGAD
Sbjct: 121 MAAHIAGRKPQLEVNMLLRDPQVCQSINDSHYNSKYFPGHKLPINVIATTDAKAALLGAD 180

Query: 211 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIA 270
           FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIP AL NPRQPF+A
Sbjct: 181 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPHALRNPRQPFVA 240

Query: 271 LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 330
           LSGPSFASE+MNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV
Sbjct: 241 LSGPSFASELMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 300

Query: 331 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 390
           LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL
Sbjct: 301 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 360

Query: 391 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 450
           SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE
Sbjct: 361 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 420

Query: 451 LTPQKAVLELMGLPQVK 465
           LTPQKAVLELMGLPQV+
Sbjct: 421 LTPQKAVLELMGLPQVE 436

BLAST of MS020762 vs. NCBI nr
Match: XP_004146469.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 758.8 bits (1958), Expect = 2.7e-215
Identity = 405/465 (87.10%), Postives = 420/465 (90.32%), Query Frame = 0

Query: 10  TFPPDLLDVTRYLPPVFL--------QSSMETPLMDLSSHSPFFTPKNHSLYHFYPSKLT 69
           TFP D LDVTRYL  VFL          SMETP MDL S++PF T  + S  H YPS+LT
Sbjct: 11  TFPTDSLDVTRYLLAVFLCDTQLSYNNPSMETPFMDLPSNNPFSTFNHPSFSHAYPSRLT 70

Query: 70  IPTLCCPNNPTTSRRRFCADTTPPEDIH--AAASDSAPERTQNRRRIVRLAWEKLVRWSR 129
           IP+L  PN PTTS  RFCADTTPP  +    A  DS PERTQNRRR+VRLAWEKLVRWSR
Sbjct: 71  IPSLSSPNYPTTSPLRFCADTTPPPILQDTDALLDSVPERTQNRRRVVRLAWEKLVRWSR 130

Query: 130 SWRFKARTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHY 189
           S RFKA+TDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRD  VCQSIND HY
Sbjct: 131 SLRFKAKTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDLLVCQSINDGHY 190

Query: 190 NCKYFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLS 249
           N KYFP HKLP+NVIATTDAKAALLGADFCFHAVPVQFSSSFLESIA HVDPH+PFISLS
Sbjct: 191 NSKYFPKHKLPINVIATTDAKAALLGADFCFHAVPVQFSSSFLESIAHHVDPHLPFISLS 250

Query: 250 KGLELNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQ 309
           KGLELN+LRMMSQI+PQALSNPRQPF+ALSGPSFASE+MNKLPTAMVVASKDKKLANAAQ
Sbjct: 251 KGLELNSLRMMSQIVPQALSNPRQPFVALSGPSFASELMNKLPTAMVVASKDKKLANAAQ 310

Query: 310 QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL 369
           QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL
Sbjct: 311 QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL 370

Query: 370 ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST 429
           ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST
Sbjct: 371 ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST 430

Query: 430 AGAVIALAQKYNVKMPVLTAVARIIDNELTPQKAVLELMGLPQVK 465
           AGAVIALAQKY VKMPVLTAVARIIDNELTPQKAVLELMGLPQV+
Sbjct: 431 AGAVIALAQKYKVKMPVLTAVARIIDNELTPQKAVLELMGLPQVE 475

BLAST of MS020762 vs. ExPASy Swiss-Prot
Match: Q949Q0 (Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLY1 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.5e-173
Identity = 321/384 (83.59%), Postives = 346/384 (90.10%), Query Frame = 0

Query: 85  PEDIHAAASDSAP----ERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLG 144
           P  I  A S S P       +++R++VR+AWEKLVRWSRS R KA+TDVLERT KV VLG
Sbjct: 35  PNVIPEADSISGPPDIINTNRDQRKVVRIAWEKLVRWSRSLRAKAKTDVLERTRKVVVLG 94

Query: 145 GGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAK 204
           GGSFGTAMAAH+A RK  LEVNML+RD  VCQSIN+ H+NCKYFP HKLP NVIATTDAK
Sbjct: 95  GGSFGTAMAAHVARRKEGLEVNMLVRDSFVCQSINENHHNCKYFPEHKLPENVIATTDAK 154

Query: 205 AALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSN 264
           AALL AD+C HAVPVQFSSSFLE IAD+VDP +PFISLSKGLELNTLRMMSQIIP AL N
Sbjct: 155 AALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLELNTLRMMSQIIPIALKN 214

Query: 265 PRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEI 324
           PRQPF+ALSGPSFA E+MN LPTAMVVASKDKKLANA QQLLASSYLRI+TSSDVTGVEI
Sbjct: 215 PRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSYLRINTSSDVTGVEI 274

Query: 325 AGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIM 384
           AGALKNVLAIAAGIV+GMNLGNNSMAALV+QGCSEIRWLATKMGAKPTTITGLSGTGDIM
Sbjct: 275 AGALKNVLAIAAGIVDGMNLGNNSMAALVSQGCSEIRWLATKMGAKPTTITGLSGTGDIM 334

Query: 385 LTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAV 444
           LTCFVNLSRNRTVGVRLGSGE LDDIL+SMNQVAEGV+TAGAVIALAQKYNVK+PVLTAV
Sbjct: 335 LTCFVNLSRNRTVGVRLGSGETLDDILTSMNQVAEGVATAGAVIALAQKYNVKLPVLTAV 394

Query: 445 ARIIDNELTPQKAVLELMGLPQVK 465
           A+IIDNELTP KAVLELM LPQ++
Sbjct: 395 AKIIDNELTPTKAVLELMNLPQIE 418

BLAST of MS020762 vs. ExPASy Swiss-Prot
Match: Q8H2J9 (Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0229800 PE=2 SV=3)

HSP 1 Score: 573.2 bits (1476), Expect = 2.7e-162
Identity = 303/380 (79.74%), Postives = 332/380 (87.37%), Query Frame = 0

Query: 90  AAASDSAPE-----RTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSF 149
           AAA+ SAP        + RRR+VR AWEKLVRWSRSWR + R+DV+E T KV VLGGGSF
Sbjct: 59  AAAAVSAPRGGGGGGGKERRRVVRKAWEKLVRWSRSWRRRNRSDVVETTRKVVVLGGGSF 118

Query: 150 GTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALL 209
           GTAMAA +A +K  LEV+MLLRD  VC+SIN +H NCKY   H+LP N+ ATT A  AL 
Sbjct: 119 GTAMAAQVAAKKADLEVSMLLRDDLVCRSINHSHINCKYLRDHRLPENITATTSASDALA 178

Query: 210 GADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQP 269
           GADFCFHAVPVQFSSSFLE I+ HVDP +PFISLSKGLELNTLR MSQIIPQAL NPRQP
Sbjct: 179 GADFCFHAVPVQFSSSFLEGISTHVDPKLPFISLSKGLELNTLRTMSQIIPQALGNPRQP 238

Query: 270 FIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGAL 329
           FI LSGPSFA E+MNKLPTAMVVASKDKKLA A QQLLAS  LRISTS+DVTGVEIAGAL
Sbjct: 239 FIVLSGPSFAIELMNKLPTAMVVASKDKKLAAAVQQLLASPNLRISTSNDVTGVEIAGAL 298

Query: 330 KNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCF 389
           KNVLAIAAGIVEGM+LGNN MAALVAQGCSEIRWLATKMGAKPTT++GLSG+GDIMLTCF
Sbjct: 299 KNVLAIAAGIVEGMHLGNNCMAALVAQGCSEIRWLATKMGAKPTTLSGLSGSGDIMLTCF 358

Query: 390 VNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARII 449
           VNLSRNR VG+RLGSGE LD+I++SMNQVAEGVSTAGAVIALAQKY+VKMPVLTAVARII
Sbjct: 359 VNLSRNRNVGLRLGSGEKLDEIMNSMNQVAEGVSTAGAVIALAQKYHVKMPVLTAVARII 418

Query: 450 DNELTPQKAVLELMGLPQVK 465
           DNELTP+KAV+ELM LPQV+
Sbjct: 419 DNELTPKKAVMELMNLPQVE 438

BLAST of MS020762 vs. ExPASy Swiss-Prot
Match: C4XLT6 (Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) OX=573370 GN=gpsA PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 3.8e-63
Identity = 141/324 (43.52%), Postives = 196/324 (60.49%), Query Frame = 0

Query: 135 KVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVI 194
           K+AV+GGGS+GT +A   A  K  L+  + +R+  V   I  +  N  Y P  KL   + 
Sbjct: 2   KIAVIGGGSWGTTLADLAA--KKGLDARLWVREQAVMNEIRSSRENSWYLPGRKLSDQLE 61

Query: 195 ATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQII 254
            +TD  A   G      AVP QF  +  +    ++  +   I  SKG+EL+TL  MSQ+ 
Sbjct: 62  VSTDPAAVAEGVSHFVFAVPCQFIRAAYQRFIKYLPKNAVVICASKGIELDTLMTMSQVC 121

Query: 255 PQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSD 314
             AL++ +  F  LSGPSFA E++ ++PTA+ +A KDKK A   Q+ L++ Y RI T++D
Sbjct: 122 QDALASIKPRFAMLSGPSFAYEVIREMPTAVTLACKDKKAAKDVQEALSTPYFRIYTTTD 181

Query: 315 VTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLS 374
           V GVE+ GA+KN++AIAAG+ +G+  G ++ AAL+ +G  E+  L   MG    T  GLS
Sbjct: 182 VRGVELGGAIKNIVAIAAGVADGLGFGGDARAALITRGLHEMSRLGKAMGGDRQTFMGLS 241

Query: 375 GTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKM 434
           G GD++LTC  +LSRNR VG+RL  G+ L DIL+ M  VAEGV TA AV AL QK  V+M
Sbjct: 242 GMGDLVLTCTGDLSRNRQVGLRLAKGQKLLDILADMKMVAEGVKTAEAVHALGQKLGVEM 301

Query: 435 PVLTAVARIIDNELTPQKAVLELM 459
           P+   V  I+  +  P KAV ELM
Sbjct: 302 PITEQVYAILYKDKDPSKAVYELM 323

BLAST of MS020762 vs. ExPASy Swiss-Prot
Match: B0SAV3 (Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) OX=355278 GN=gpsA PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 9.4e-62
Identity = 135/325 (41.54%), Postives = 197/325 (60.62%), Query Frame = 0

Query: 135 KVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVI 194
           K+ ++G GSFGTA+ + +A +    +V +  R  +  +SIN+ H N K+ P   LP  + 
Sbjct: 2   KIGIIGAGSFGTALGSILADK--GYDVTLWTRSEEQARSINENHMNSKHMPDLVLPDRLK 61

Query: 195 ATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQII 254
           A+TD    +   D    A P    S  L+ I DH+ P VP +S SKG+E  +LR++S+I 
Sbjct: 62  ASTDLIQVVKDKDMIVSAPPSHALSGILKEIKDHIPPKVPIVSASKGIENESLRLVSEIF 121

Query: 255 PQALSNPRQPFIA-LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSS 314
              L       ++ LSGPSFA E++ ++PT + +ASK++  A   Q++ + +Y R   + 
Sbjct: 122 ESELPGQFHSQLSYLSGPSFAKEMVKRVPTIVSIASKNEATAKRVQEIFSFTYFRTYWTP 181

Query: 315 DVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGL 374
           DV GVE+ GALKNV+AIAAG+ +G+  G N+ AAL+ +G +EI  +  KMGA P T  G 
Sbjct: 182 DVVGVEVGGALKNVIAIAAGVADGLGFGQNTRAALITRGLNEITRMGIKMGADPMTFLGP 241

Query: 375 SGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVK 434
           SG GD++LTC    SRNRTVG RLG GE+L +ILSSMN+VAEGV T  +   LA K  V+
Sbjct: 242 SGMGDLVLTCCGEASRNRTVGFRLGKGESLKEILSSMNEVAEGVKTTLSTKNLADKLGVE 301

Query: 435 MPVLTAVARIIDNELTPQKAVLELM 459
           M +   V  ++  +  P++ V  LM
Sbjct: 302 MAITQEVYHMLYEDKDPKEVVRALM 324

BLAST of MS020762 vs. ExPASy Swiss-Prot
Match: B0SKD9 (Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) OX=456481 GN=gpsA PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 9.4e-62
Identity = 135/325 (41.54%), Postives = 197/325 (60.62%), Query Frame = 0

Query: 135 KVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVI 194
           K+ ++G GSFGTA+ + +A +    +V +  R  +  +SIN+ H N K+ P   LP  + 
Sbjct: 2   KIGIIGAGSFGTALGSILADK--GYDVTLWTRSEEQARSINENHMNSKHMPDLVLPDRLK 61

Query: 195 ATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQII 254
           A+TD    +   D    A P    S  L+ I DH+ P VP +S SKG+E  +LR++S+I 
Sbjct: 62  ASTDLIQVVKDKDMIVSAPPSHALSGILKEIKDHIPPKVPIVSASKGIENESLRLVSEIF 121

Query: 255 PQALSNPRQPFIA-LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSS 314
              L       ++ LSGPSFA E++ ++PT + +ASK++  A   Q++ + +Y R   + 
Sbjct: 122 ESELPGQFHSQLSYLSGPSFAKEMVKRVPTIVSIASKNEATAKRVQEIFSFTYFRTYWTP 181

Query: 315 DVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGL 374
           DV GVE+ GALKNV+AIAAG+ +G+  G N+ AAL+ +G +EI  +  KMGA P T  G 
Sbjct: 182 DVVGVEVGGALKNVIAIAAGVADGLGFGQNTRAALITRGLNEITRMGIKMGADPMTFLGP 241

Query: 375 SGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVK 434
           SG GD++LTC    SRNRTVG RLG GE+L +ILSSMN+VAEGV T  +   LA K  V+
Sbjct: 242 SGMGDLVLTCCGEASRNRTVGFRLGKGESLKEILSSMNEVAEGVKTTLSTKNLADKLGVE 301

Query: 435 MPVLTAVARIIDNELTPQKAVLELM 459
           M +   V  ++  +  P++ V  LM
Sbjct: 302 MAITQEVYHMLYEDKDPKEVVRALM 324

BLAST of MS020762 vs. ExPASy TrEMBL
Match: A0A6J1CYX2 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LOC111015864 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.6e-240
Identity = 431/434 (99.31%), Postives = 433/434 (99.77%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPPEDIHA 90
           METPLMDLSSHSPFFTPKNHSLYHFYPSK+TIPTLCCPNNPTTS RRFCADTTPPEDIHA
Sbjct: 1   METPLMDLSSHSPFFTPKNHSLYHFYPSKITIPTLCCPNNPTTSLRRFCADTTPPEDIHA 60

Query: 91  AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA 150
           AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA
Sbjct: 61  AASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTAMAA 120

Query: 151 HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF 210
           HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF
Sbjct: 121 HIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGADFCF 180

Query: 211 HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG 270
           HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG
Sbjct: 181 HAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIALSG 240

Query: 271 PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI 330
           PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI
Sbjct: 241 PSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNVLAI 300

Query: 331 AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN 390
           AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN
Sbjct: 301 AAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRN 360

Query: 391 RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP 450
           RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP
Sbjct: 361 RTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNELTP 420

Query: 451 QKAVLELMGLPQVK 465
           QKAVLELMGLPQV+
Sbjct: 421 QKAVLELMGLPQVE 434

BLAST of MS020762 vs. ExPASy TrEMBL
Match: A0A1S3C535 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103496755 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.0e-217
Identity = 405/464 (87.28%), Postives = 422/464 (90.95%), Query Frame = 0

Query: 11  FPPDLLDVTRYLPPVFL--------QSSMETPLMDLSSHSPFFTPKNHSLYHFYPSKLTI 70
           FPPD LD+TRYL  VFL          SMETP MDL S++PF T  + S  H YPS+LTI
Sbjct: 14  FPPDPLDITRYLLAVFLCDTQLSYNNPSMETPFMDLPSNNPFSTLNHPSFSHTYPSRLTI 73

Query: 71  PTLCCPNNPTTSRRRFCADTTPPEDIH--AAASDSAPERTQNRRRIVRLAWEKLVRWSRS 130
           P+L  PN PTTS  RFCA+TTPP  +    A  DS PERTQNRRR+VR+AWEKLVRWSRS
Sbjct: 74  PSLSSPNYPTTSPLRFCANTTPPPILQDTDALLDSGPERTQNRRRVVRIAWEKLVRWSRS 133

Query: 131 WRFKARTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYN 190
            RFKA+TDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSIND HYN
Sbjct: 134 LRFKAKTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDGHYN 193

Query: 191 CKYFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSK 250
            KYFP HKLP+NVIATTDAKAALLGADFCFHAVPVQFSSSFLESIA HVDPH+PFISLSK
Sbjct: 194 SKYFPKHKLPINVIATTDAKAALLGADFCFHAVPVQFSSSFLESIAHHVDPHLPFISLSK 253

Query: 251 GLELNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQ 310
           GLELN+LRMMSQIIPQALSNPRQPF+ALSGPSFASE+MNKLPTAMVVASKDKKLANAAQQ
Sbjct: 254 GLELNSLRMMSQIIPQALSNPRQPFVALSGPSFASELMNKLPTAMVVASKDKKLANAAQQ 313

Query: 311 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA 370
           LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA
Sbjct: 314 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLA 373

Query: 371 TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA 430
           TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA
Sbjct: 374 TKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTA 433

Query: 431 GAVIALAQKYNVKMPVLTAVARIIDNELTPQKAVLELMGLPQVK 465
           GAVIALAQKY VKMPVLTAVARIIDNELTPQKAVLELMGLPQV+
Sbjct: 434 GAVIALAQKYKVKMPVLTAVARIIDNELTPQKAVLELMGLPQVE 477

BLAST of MS020762 vs. ExPASy TrEMBL
Match: A0A6J1K6S7 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111491179 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.8e-216
Identity = 397/437 (90.85%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPP---ED 90
           METPL+DLSS + FFTPKN+SLY+  PSKLT  +LCCP+ PTTS RRFCAD +PP   ED
Sbjct: 1   METPLLDLSSRNAFFTPKNYSLYYTKPSKLTNLSLCCPDKPTTSHRRFCADESPPPILED 60

Query: 91  IHAAASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTA 150
                 D   E+T NRRRIVRLAWEKLVRWSRSWRFKA+TDVLERTNKVAVLGGGSFGTA
Sbjct: 61  TDVVL-DLVTEKTPNRRRIVRLAWEKLVRWSRSWRFKAKTDVLERTNKVAVLGGGSFGTA 120

Query: 151 MAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGAD 210
           MAAHIAGRKPQLEVNMLLRDPQVCQSIND+HYN KYFP HKLP+NVIATTDAKAALLGAD
Sbjct: 121 MAAHIAGRKPQLEVNMLLRDPQVCQSINDSHYNSKYFPRHKLPINVIATTDAKAALLGAD 180

Query: 211 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIA 270
           FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIP AL NPRQPF+A
Sbjct: 181 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPHALRNPRQPFVA 240

Query: 271 LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 330
           LSGPSFASE+MNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV
Sbjct: 241 LSGPSFASELMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 300

Query: 331 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 390
           LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL
Sbjct: 301 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 360

Query: 391 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 450
           SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE
Sbjct: 361 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 420

Query: 451 LTPQKAVLELMGLPQVK 465
           LTPQKAVLELMGLPQV+
Sbjct: 421 LTPQKAVLELMGLPQVE 436

BLAST of MS020762 vs. ExPASy TrEMBL
Match: A0A6J1FQT6 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111447365 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.0e-215
Identity = 397/437 (90.85%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 31  METPLMDLSSHSPFFTPKNHSLYHFYPSKLTIPTLCCPNNPTTSRRRFCADTTPP---ED 90
           METPL+DLSS + FFTPKN+SLY+  PSKLT  +LCCP+ PTTS RRFCAD +PP   ED
Sbjct: 1   METPLLDLSSRNAFFTPKNYSLYYTKPSKLTNFSLCCPDKPTTSHRRFCADESPPPILED 60

Query: 91  IHAAASDSAPERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLGGGSFGTA 150
                 D   E+T NRRRIVRLAWEKLVRWSRSWRFKA+TDVLERTNKVAVLGGGSFGTA
Sbjct: 61  TDVVL-DLVTEKTPNRRRIVRLAWEKLVRWSRSWRFKAKTDVLERTNKVAVLGGGSFGTA 120

Query: 151 MAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAKAALLGAD 210
           MAAHIAGRKPQLEVNMLLRDPQVCQSIND+HYN KYFP HKLP+NVIATTDAKAALLGAD
Sbjct: 121 MAAHIAGRKPQLEVNMLLRDPQVCQSINDSHYNSKYFPGHKLPINVIATTDAKAALLGAD 180

Query: 211 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSNPRQPFIA 270
           FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIP AL NPRQPF+A
Sbjct: 181 FCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPHALRNPRQPFVA 240

Query: 271 LSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 330
           LSGPSFASE+MNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV
Sbjct: 241 LSGPSFASELMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEIAGALKNV 300

Query: 331 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 390
           LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL
Sbjct: 301 LAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNL 360

Query: 391 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 450
           SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE
Sbjct: 361 SRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAVARIIDNE 420

Query: 451 LTPQKAVLELMGLPQVK 465
           LTPQKAVLELMGLPQV+
Sbjct: 421 LTPQKAVLELMGLPQVE 436

BLAST of MS020762 vs. ExPASy TrEMBL
Match: A0A0A0KPB1 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_5G223030 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 405/465 (87.10%), Postives = 420/465 (90.32%), Query Frame = 0

Query: 10  TFPPDLLDVTRYLPPVFL--------QSSMETPLMDLSSHSPFFTPKNHSLYHFYPSKLT 69
           TFP D LDVTRYL  VFL          SMETP MDL S++PF T  + S  H YPS+LT
Sbjct: 11  TFPTDSLDVTRYLLAVFLCDTQLSYNNPSMETPFMDLPSNNPFSTFNHPSFSHAYPSRLT 70

Query: 70  IPTLCCPNNPTTSRRRFCADTTPPEDIH--AAASDSAPERTQNRRRIVRLAWEKLVRWSR 129
           IP+L  PN PTTS  RFCADTTPP  +    A  DS PERTQNRRR+VRLAWEKLVRWSR
Sbjct: 71  IPSLSSPNYPTTSPLRFCADTTPPPILQDTDALLDSVPERTQNRRRVVRLAWEKLVRWSR 130

Query: 130 SWRFKARTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHY 189
           S RFKA+TDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRD  VCQSIND HY
Sbjct: 131 SLRFKAKTDVLERTNKVAVLGGGSFGTAMAAHIAGRKPQLEVNMLLRDLLVCQSINDGHY 190

Query: 190 NCKYFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLS 249
           N KYFP HKLP+NVIATTDAKAALLGADFCFHAVPVQFSSSFLESIA HVDPH+PFISLS
Sbjct: 191 NSKYFPKHKLPINVIATTDAKAALLGADFCFHAVPVQFSSSFLESIAHHVDPHLPFISLS 250

Query: 250 KGLELNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQ 309
           KGLELN+LRMMSQI+PQALSNPRQPF+ALSGPSFASE+MNKLPTAMVVASKDKKLANAAQ
Sbjct: 251 KGLELNSLRMMSQIVPQALSNPRQPFVALSGPSFASELMNKLPTAMVVASKDKKLANAAQ 310

Query: 310 QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL 369
           QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL
Sbjct: 311 QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL 370

Query: 370 ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST 429
           ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST
Sbjct: 371 ATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVST 430

Query: 430 AGAVIALAQKYNVKMPVLTAVARIIDNELTPQKAVLELMGLPQVK 465
           AGAVIALAQKY VKMPVLTAVARIIDNELTPQKAVLELMGLPQV+
Sbjct: 431 AGAVIALAQKYKVKMPVLTAVARIIDNELTPQKAVLELMGLPQVE 475

BLAST of MS020762 vs. TAIR 10
Match: AT2G40690.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 609.4 bits (1570), Expect = 2.5e-174
Identity = 321/384 (83.59%), Postives = 346/384 (90.10%), Query Frame = 0

Query: 85  PEDIHAAASDSAP----ERTQNRRRIVRLAWEKLVRWSRSWRFKARTDVLERTNKVAVLG 144
           P  I  A S S P       +++R++VR+AWEKLVRWSRS R KA+TDVLERT KV VLG
Sbjct: 35  PNVIPEADSISGPPDIINTNRDQRKVVRIAWEKLVRWSRSLRAKAKTDVLERTRKVVVLG 94

Query: 145 GGSFGTAMAAHIAGRKPQLEVNMLLRDPQVCQSINDTHYNCKYFPTHKLPVNVIATTDAK 204
           GGSFGTAMAAH+A RK  LEVNML+RD  VCQSIN+ H+NCKYFP HKLP NVIATTDAK
Sbjct: 95  GGSFGTAMAAHVARRKEGLEVNMLVRDSFVCQSINENHHNCKYFPEHKLPENVIATTDAK 154

Query: 205 AALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGLELNTLRMMSQIIPQALSN 264
           AALL AD+C HAVPVQFSSSFLE IAD+VDP +PFISLSKGLELNTLRMMSQIIP AL N
Sbjct: 155 AALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLELNTLRMMSQIIPIALKN 214

Query: 265 PRQPFIALSGPSFASEIMNKLPTAMVVASKDKKLANAAQQLLASSYLRISTSSDVTGVEI 324
           PRQPF+ALSGPSFA E+MN LPTAMVVASKDKKLANA QQLLASSYLRI+TSSDVTGVEI
Sbjct: 215 PRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSYLRINTSSDVTGVEI 274

Query: 325 AGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWLATKMGAKPTTITGLSGTGDIM 384
           AGALKNVLAIAAGIV+GMNLGNNSMAALV+QGCSEIRWLATKMGAKPTTITGLSGTGDIM
Sbjct: 275 AGALKNVLAIAAGIVDGMNLGNNSMAALVSQGCSEIRWLATKMGAKPTTITGLSGTGDIM 334

Query: 385 LTCFVNLSRNRTVGVRLGSGENLDDILSSMNQVAEGVSTAGAVIALAQKYNVKMPVLTAV 444
           LTCFVNLSRNRTVGVRLGSGE LDDIL+SMNQVAEGV+TAGAVIALAQKYNVK+PVLTAV
Sbjct: 335 LTCFVNLSRNRTVGVRLGSGETLDDILTSMNQVAEGVATAGAVIALAQKYNVKLPVLTAV 394

Query: 445 ARIIDNELTPQKAVLELMGLPQVK 465
           A+IIDNELTP KAVLELM LPQ++
Sbjct: 395 AKIIDNELTPTKAVLELMNLPQIE 418

BLAST of MS020762 vs. TAIR 10
Match: AT5G40610.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 114.0 bits (284), Expect = 3.2e-25
Identity = 103/342 (30.12%), Postives = 161/342 (47.08%), Query Frame = 0

Query: 134 NKVAVLGGGSFGTAMAAHIAGRKPQL-----EVNM------LLRDPQVCQSINDTHYNCK 193
           +KV V+G G++G+  A  IA    +L     EV M      L    ++   IN T+ N K
Sbjct: 55  SKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENVK 114

Query: 194 YFPTHKLPVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVPFISLSKGL 253
           Y P  KL  NV+A  D + A+  A+      P QF     + +   +   V  ISL KG+
Sbjct: 115 YLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGM 174

Query: 254 ELNTLR--MMSQIIPQALSNPRQPFIALSGPSFASEI-MNKLPTAMVVASKDKKLANAAQ 313
           E+      M+S +I + L         L G + A+EI + K   A V     +++A+   
Sbjct: 175 EVKKEGPCMISSLISKQLG---INCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWV 234

Query: 314 QLLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGMNLGNNSMAALVAQGCSEIRWL 373
           QL ++ Y  ++   DV GVE+ G LKNV+AIAAG V+G+ +GNN+ AA++  G  E++ L
Sbjct: 235 QLFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGLEMGNNTKAAIMRIGLREMKAL 294

Query: 374 ATKM--GAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGENLDDILSS---MNQVA 433
           +  +    K +T     G  D++ TC    +R          G+   D L +     Q  
Sbjct: 295 SKLLFPSVKDSTFFESCGVADVITTCLGGRNRRVAEAFAKSRGKRSFDELEAEMLQGQKL 354

Query: 434 EGVSTAGAVIALAQK--YNVKMPVLTAVARIIDNELTPQKAV 455
           +GVSTA  V  + +   +    P+ + V +I    L P+  V
Sbjct: 355 QGVSTAREVYEVLKHCGWLEMFPLFSTVHQICTGRLQPEAIV 393

BLAST of MS020762 vs. TAIR 10
Match: AT2G41540.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.8e-07
Identity = 67/260 (25.77%), Postives = 116/260 (44.62%), Query Frame = 0

Query: 190 PVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVP---FISLSKGLE--- 249
           P+ V+  T+ + A+  AD   + +P   +    E I+ +    +     ISLSKG+E   
Sbjct: 145 PLKVV--TNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETAL 204

Query: 250 --LNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNK-LPTAMVVASKDKKLANAAQQ 309
             +  +   +++I QA   P    + L GP+ A+EI NK    A +  +   K      +
Sbjct: 205 EPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA--AKWRKPLAK 264

Query: 310 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGM-NLGNNSMAALVAQGCSEIRWL 369
            L   +  +  +SD+   E+ G LKNV AI AG+V  + N    S +   A   SE+ ++
Sbjct: 265 FLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFI 324

Query: 370 ATKMGAKPTTITGLSGTGDIMLTCFVNL--SRNRTVGVRLGSGE---NLDDILSSMNQVA 429
              +  +P  +      G ++   +V L   RN   G  L  GE   ++ D +S    + 
Sbjct: 325 THLLAEEPEKL-----AGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGKGMI- 384

Query: 430 EGVSTAGAVIALAQKYNVKM 435
           +GVS  GA   L  + ++ +
Sbjct: 385 QGVSAVGAFYQLLSQSSLSI 394

BLAST of MS020762 vs. TAIR 10
Match: AT2G41540.2 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.8e-07
Identity = 67/260 (25.77%), Postives = 116/260 (44.62%), Query Frame = 0

Query: 190 PVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVP---FISLSKGLE--- 249
           P+ V+  T+ + A+  AD   + +P   +    E I+ +    +     ISLSKG+E   
Sbjct: 145 PLKVV--TNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETAL 204

Query: 250 --LNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNK-LPTAMVVASKDKKLANAAQQ 309
             +  +   +++I QA   P    + L GP+ A+EI NK    A +  +   K      +
Sbjct: 205 EPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA--AKWRKPLAK 264

Query: 310 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGM-NLGNNSMAALVAQGCSEIRWL 369
            L   +  +  +SD+   E+ G LKNV AI AG+V  + N    S +   A   SE+ ++
Sbjct: 265 FLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFI 324

Query: 370 ATKMGAKPTTITGLSGTGDIMLTCFVNL--SRNRTVGVRLGSGE---NLDDILSSMNQVA 429
              +  +P  +      G ++   +V L   RN   G  L  GE   ++ D +S    + 
Sbjct: 325 THLLAEEPEKL-----AGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGKGMI- 384

Query: 430 EGVSTAGAVIALAQKYNVKM 435
           +GVS  GA   L  + ++ +
Sbjct: 385 QGVSAVGAFYQLLSQSSLSI 394

BLAST of MS020762 vs. TAIR 10
Match: AT2G41540.3 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.8e-07
Identity = 67/260 (25.77%), Postives = 116/260 (44.62%), Query Frame = 0

Query: 190 PVNVIATTDAKAALLGADFCFHAVPVQFSSSFLESIADHVDPHVP---FISLSKGLE--- 249
           P+ V+  T+ + A+  AD   + +P   +    E I+ +    +     ISLSKG+E   
Sbjct: 145 PLKVV--TNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETAL 204

Query: 250 --LNTLRMMSQIIPQALSNPRQPFIALSGPSFASEIMNK-LPTAMVVASKDKKLANAAQQ 309
             +  +   +++I QA   P    + L GP+ A+EI NK    A +  +   K      +
Sbjct: 205 EPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA--AKWRKPLAK 264

Query: 310 LLASSYLRISTSSDVTGVEIAGALKNVLAIAAGIVEGM-NLGNNSMAALVAQGCSEIRWL 369
            L   +  +  +SD+   E+ G LKNV AI AG+V  + N    S +   A   SE+ ++
Sbjct: 265 FLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFI 324

Query: 370 ATKMGAKPTTITGLSGTGDIMLTCFVNL--SRNRTVGVRLGSGE---NLDDILSSMNQVA 429
              +  +P  +      G ++   +V L   RN   G  L  GE   ++ D +S    + 
Sbjct: 325 THLLAEEPEKL-----AGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGKGMI- 384

Query: 430 EGVSTAGAVIALAQKYNVKM 435
           +GVS  GA   L  + ++ +
Sbjct: 385 QGVSAVGAFYQLLSQSSLSI 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146729.15.4e-24099.31glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Momordica charanti... [more]
XP_008456964.18.4e-21787.28PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucumis... [more]
XP_022995739.11.2e-21590.85glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucurbita maxima][more]
XP_022942269.12.1e-21590.85glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Cucurbita moschata... [more]
XP_004146469.12.7e-21587.10glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic isoform X1 [Cucumis... [more]
Match NameE-valueIdentityDescription
Q949Q03.5e-17383.59Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic OS=Arabidopsis thal... [more]
Q8H2J92.7e-16279.74Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic OS=Oryza sativa subsp... [more]
C4XLT63.8e-6343.52Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Desulfovibrio magneticus (strain... [more]
B0SAV39.4e-6241.54Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Leptospira biflexa serovar Patoc... [more]
B0SKD99.4e-6241.54Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Leptospira biflexa serovar Patoc... [more]
Match NameE-valueIdentityDescription
A0A6J1CYX22.6e-24099.31Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3C5354.0e-21787.28Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103496... [more]
A0A6J1K6S75.8e-21690.85Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FQT61.0e-21590.85Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0KPB11.3e-21587.10Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
Match NameE-valueIdentityDescription
AT2G40690.12.5e-17483.59NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
AT5G40610.13.2e-2530.12NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
AT2G41540.11.8e-0725.776-phosphogluconate dehydrogenase family protein [more]
AT2G41540.21.8e-0725.776-phosphogluconate dehydrogenase family protein [more]
AT2G41540.31.8e-0725.776-phosphogluconate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPRINTSPR00077GPDHDRGNASEcoord: 190..217
score: 38.31
coord: 332..356
score: 40.61
coord: 267..287
score: 28.28
coord: 138..155
score: 36.03
coord: 307..331
score: 47.03
coord: 372..389
score: 34.68
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 103..462
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPROSITEPS00957NAD_G3PDHcoord: 322..343
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentHAMAPMF_00394NAD_Glyc3P_dehydrogcoord: 134..456
score: 32.181812
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 323..464
e-value: 1.1E-47
score: 164.0
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 314..454
e-value: 3.5E-48
score: 163.5
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 135..294
e-value: 2.5E-41
score: 141.3
NoneNo IPR availableGENE3D3.40.50.720coord: 120..321
e-value: 2.0E-62
score: 212.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..99
NoneNo IPR availablePANTHERPTHR11728:SF1GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)] 2, CHLOROPLASTICcoord: 103..462
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 133..314
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 313..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020762.1MS020762.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor