MS018721 (gene) Bitter gourd (TR) v1

BLAST of MS018721 vs. NCBI nr
Match: XP_022146458.1 (uncharacterized protein LOC111015671 [Momordica charantia])

HSP 1 Score: 715.7 bits (1846), Expect = 1.9e-202
Identity = 327/336 (97.32%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIG VPWGKIVDS
Sbjct: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGRVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           KKFIYCHNNIVNWNDSACEEAFHNAKR FWA TNGHRCDIYLPDPDKYIEKIDWNPEIDS
Sbjct: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRRFWAHTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIED GYVGNAWEHGIQCIAN
Sbjct: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDPGYVGNAWEHGIQCIAN 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
           TGEGWNQRNLSD GKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR
Sbjct: 181 TGEGWNQRNLSDPGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240

Query: 241 GMVDGSWRDKVHQGGATSWKTKGFASDTRNNTRGWNQKQRACNFDHYNRPWSNSYNRNVR 300
           GMVDGSWR+KVHQGGATSWKTKGFASDTRNN  GWNQKQRACNFDHYNRPWSNSYNRNVR
Sbjct: 241 GMVDGSWREKVHQGGATSWKTKGFASDTRNNMWGWNQKQRACNFDHYNRPWSNSYNRNVR 300

Query: 301 NLTDRIQPNNQGNEHGWKYQWYGKRPKDAEFVNIEW 337
           NLTDRIQPNNQGNE GWKYQWYGKRPKDAEFVNIEW
Sbjct: 301 NLTDRIQPNNQGNEQGWKYQWYGKRPKDAEFVNIEW 336

BLAST of MS018721 vs. NCBI nr
Match: XP_038906443.1 (uncharacterized protein LOC120092427 [Benincasa hispida])

HSP 1 Score: 512.7 bits (1319), Expect = 2.4e-141
Identity = 241/333 (72.37%), Postives = 272/333 (81.68%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKP WSAIRNYDHDPPLE+W+DGIPLWEKKFC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRYQKPQWSAIRNYDHDPPLEYWRDGIPLWEKKFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACEEAF NAKR +W + NGH+ DI LPDPDKYIE+IDW+P++DS
Sbjct: 61  KNFIYCHTNVVNWDDSACEEAFDNAKRRYWVEINGHQSDICLPDPDKYIEEIDWSPDMDS 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           +MIEELDWAY  PN+ + DD LE KNKRT+NSSSVW EGHIEDSG VGN WEH  Q   N
Sbjct: 121 KMIEELDWAYHTPNMKQRDDWLECKNKRTRNSSSVWTEGHIEDSGDVGNPWEHDKQFTEN 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
            G+G +Q NLSD GK NNDGNPWDSS+ Q N G+VDSAW+ K  Q  TSW  KG +SDAR
Sbjct: 181 RGQGCSQWNLSDFGKVNNDGNPWDSSIDQRNRGMVDSAWKVKANQVATSWKNKGSASDAR 240

Query: 241 GMVDGSWRDKVHQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRNV 300
           G+VD + RDKVHQGG  SWKTKGFASD RNN+  W++ Q+ A NFDHYNRP +NSYN NV
Sbjct: 241 GVVDNASRDKVHQGGNASWKTKGFASDARNNS--WSRHQQGASNFDHYNRPGNNSYNHNV 300

Query: 301 RNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           R+LTDR +PN  GN   WKYQ+ YGKRPKDAEF
Sbjct: 301 RHLTDRTRPNIHGNNQDWKYQYRYGKRPKDAEF 331

BLAST of MS018721 vs. NCBI nr
Match: XP_022993174.1 (uncharacterized protein LOC111489271 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 8.1e-137
Identity = 230/334 (68.86%), Postives = 269/334 (80.54%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSA+RNYD DPPLE+W+DGIPLWEK FC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRNQKPPWSALRNYDQDPPLEYWRDGIPLWEKTFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACEEAFHNAKR +WA  NGH+CDI LPDPDKYIE+IDW+PE+D 
Sbjct: 61  KNFIYCHTNVVNWDDSACEEAFHNAKRRYWAAINGHQCDINLPDPDKYIEQIDWSPEMDP 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           EMIEELDWAY+ PN+ + DD LE KNKRT+NSSSVW E HIED G+VGN WEHG Q    
Sbjct: 121 EMIEELDWAYYNPNMKQHDDWLECKNKRTRNSSSVWTESHIEDPGHVGNPWEHGNQFTET 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
            G+GW+Q NLS+S + NNDGN WD+ +   N G+VD+AW+DK  Q  TSW  KGFS DAR
Sbjct: 181 KGQGWSQWNLSESRQVNNDGNHWDNIIDPKNRGMVDTAWKDKGNQVVTSWQNKGFSRDAR 240

Query: 241 GMVDGSWRDKV-HQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRN 300
            MVD +WR  V HQ GA +WKTKGFASD RNN+  W++ Q+ A NFDHYNRP +++YN+N
Sbjct: 241 SMVDNAWRANVQHQDGAATWKTKGFASDARNNS--WSRHQQCASNFDHYNRPGNSNYNQN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           VRNL DR+ PN  GN+  WK ++ YGKRPKDA+F
Sbjct: 301 VRNLPDRMPPNIHGNKQDWKCKYRYGKRPKDAQF 332

BLAST of MS018721 vs. NCBI nr
Match: XP_023551570.1 (uncharacterized protein LOC111809355 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.9 bits (1278), Expect = 1.4e-136
Identity = 229/334 (68.56%), Postives = 269/334 (80.54%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSA+RNYD DPPLE+W+DGIPLWEKKFC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRNQKPPWSALRNYDQDPPLEYWRDGIPLWEKKFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACEEAFHNAKR +WA  NGH+CDI LPDPDKYIE+IDW+PE+D 
Sbjct: 61  KNFIYCHTNVVNWDDSACEEAFHNAKRRYWAAINGHQCDINLPDPDKYIEQIDWSPEMDP 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           E+IEELDWAY+ PN+ + DD LE KNKRT+NSSSVW E HIED G+VGN WEHG Q    
Sbjct: 121 ELIEELDWAYYNPNMKQQDDWLECKNKRTRNSSSVWTESHIEDPGHVGNPWEHGNQFTET 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
             +GW+Q NLS+S + NNDGNPWD+ +   N G+VD+AW+DK  Q  TSW  KGFS DAR
Sbjct: 181 KRQGWSQWNLSESRQVNNDGNPWDNIIDPRNRGMVDTAWKDKGNQVVTSWKNKGFSRDAR 240

Query: 241 GMVDGSWRDKV-HQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRN 300
            MVD +WR  V +Q GA +WKTKGF SD RNN+  W++ Q+ A NFDHYNRP +++YNRN
Sbjct: 241 SMVDNAWRANVQYQDGAATWKTKGFTSDARNNS--WSRHQQGASNFDHYNRPGNSNYNRN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           VRNL DR+  N  GN+  WKY++ YGKRPKDA+F
Sbjct: 301 VRNLPDRMPSNIHGNKQEWKYKYRYGKRPKDAQF 332

BLAST of MS018721 vs. NCBI nr
Match: KAG6579118.1 (hypothetical protein SDJN03_23566, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494.6 bits (1272), Expect = 6.8e-136
Identity = 228/334 (68.26%), Postives = 268/334 (80.24%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSA+RNYD DPPLE+W+DGIPLWEK FC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRNQKPPWSALRNYDQDPPLEYWRDGIPLWEKTFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACE AFHNAKR +WA  NGH+CDI LPDPDKYIE+IDW+PE+D 
Sbjct: 61  KNFIYCHTNVVNWDDSACEAAFHNAKRRYWAAINGHQCDINLPDPDKYIEQIDWSPEMDP 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           E+IEELDWAY+ PN+ + DD LE KNKRT+NSSSVW E HIED G+VGN WEHG Q    
Sbjct: 121 ELIEELDWAYYNPNMKQQDDWLECKNKRTRNSSSVWTESHIEDPGHVGNPWEHGNQFTET 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
             +GW+Q NLS+S + N DGNPWD+ +   N G+VD+AW+DK  Q  TSW  KGFS DAR
Sbjct: 181 KRQGWSQWNLSESRQVNKDGNPWDNIIDPRNRGMVDTAWKDKGNQVVTSWKNKGFSRDAR 240

Query: 241 GMVDGSWRDKV-HQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRN 300
            MVD +WR  V +Q GA +WKTKGFASD RNN+  W++ Q+ A NFDHYNRP +++YNRN
Sbjct: 241 SMVDNAWRANVQYQDGAATWKTKGFASDARNNS--WSRHQQGASNFDHYNRPGNSNYNRN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           VRNL DR+ PN  GN+  WKY++ YGKRPKDA+F
Sbjct: 301 VRNLPDRMPPNIHGNKQEWKYKYRYGKRPKDAQF 332

BLAST of MS018721 vs. ExPASy TrEMBL
Match: A0A6J1CYM6 (uncharacterized protein LOC111015671 OS=Momordica charantia OX=3673 GN=LOC111015671 PE=4 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 9.1e-203
Identity = 327/336 (97.32%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIG VPWGKIVDS
Sbjct: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGRVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           KKFIYCHNNIVNWNDSACEEAFHNAKR FWA TNGHRCDIYLPDPDKYIEKIDWNPEIDS
Sbjct: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRRFWAHTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIED GYVGNAWEHGIQCIAN
Sbjct: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDPGYVGNAWEHGIQCIAN 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
           TGEGWNQRNLSD GKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR
Sbjct: 181 TGEGWNQRNLSDPGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240

Query: 241 GMVDGSWRDKVHQGGATSWKTKGFASDTRNNTRGWNQKQRACNFDHYNRPWSNSYNRNVR 300
           GMVDGSWR+KVHQGGATSWKTKGFASDTRNN  GWNQKQRACNFDHYNRPWSNSYNRNVR
Sbjct: 241 GMVDGSWREKVHQGGATSWKTKGFASDTRNNMWGWNQKQRACNFDHYNRPWSNSYNRNVR 300

Query: 301 NLTDRIQPNNQGNEHGWKYQWYGKRPKDAEFVNIEW 337
           NLTDRIQPNNQGNE GWKYQWYGKRPKDAEFVNIEW
Sbjct: 301 NLTDRIQPNNQGNEQGWKYQWYGKRPKDAEFVNIEW 336

BLAST of MS018721 vs. ExPASy TrEMBL
Match: A0A6J1JZH0 (uncharacterized protein LOC111489271 OS=Cucurbita maxima OX=3661 GN=LOC111489271 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.9e-137
Identity = 230/334 (68.86%), Postives = 269/334 (80.54%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSA+RNYD DPPLE+W+DGIPLWEK FC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRNQKPPWSALRNYDQDPPLEYWRDGIPLWEKTFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACEEAFHNAKR +WA  NGH+CDI LPDPDKYIE+IDW+PE+D 
Sbjct: 61  KNFIYCHTNVVNWDDSACEEAFHNAKRRYWAAINGHQCDINLPDPDKYIEQIDWSPEMDP 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           EMIEELDWAY+ PN+ + DD LE KNKRT+NSSSVW E HIED G+VGN WEHG Q    
Sbjct: 121 EMIEELDWAYYNPNMKQHDDWLECKNKRTRNSSSVWTESHIEDPGHVGNPWEHGNQFTET 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
            G+GW+Q NLS+S + NNDGN WD+ +   N G+VD+AW+DK  Q  TSW  KGFS DAR
Sbjct: 181 KGQGWSQWNLSESRQVNNDGNHWDNIIDPKNRGMVDTAWKDKGNQVVTSWQNKGFSRDAR 240

Query: 241 GMVDGSWRDKV-HQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRN 300
            MVD +WR  V HQ GA +WKTKGFASD RNN+  W++ Q+ A NFDHYNRP +++YN+N
Sbjct: 241 SMVDNAWRANVQHQDGAATWKTKGFASDARNNS--WSRHQQCASNFDHYNRPGNSNYNQN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           VRNL DR+ PN  GN+  WK ++ YGKRPKDA+F
Sbjct: 301 VRNLPDRMPPNIHGNKQDWKCKYRYGKRPKDAQF 332

BLAST of MS018721 vs. ExPASy TrEMBL
Match: A0A6J1FGI8 (uncharacterized protein LOC111445202 OS=Cucurbita moschata OX=3662 GN=LOC111445202 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.6e-136
Identity = 228/334 (68.26%), Postives = 267/334 (79.94%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSA RNYD DPPLE+W+DGIPLWEK FC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRNQKPPWSASRNYDQDPPLEYWRDGIPLWEKTFCKEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+VNW+DSACE AFHNAKR +WA  NGH+CDI LPDPDKYIE+IDW+PE+D 
Sbjct: 61  KNFIYCHTNVVNWDDSACEAAFHNAKRRYWAAINGHQCDINLPDPDKYIEQIDWSPEMDP 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           E+IEELDWAY+ PN+ + DD LE KNKRT+NSSSVW E HIED G+VGN WEHG Q    
Sbjct: 121 ELIEELDWAYYNPNMKQQDDWLECKNKRTRNSSSVWTESHIEDPGHVGNPWEHGNQFTET 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
             +GW+Q NLS+S + N DGNPWD+ +   N G+VD+AW+DK  Q  TSW  KGFS DAR
Sbjct: 181 KRQGWSQWNLSESRQVNKDGNPWDNIIDPRNRGMVDTAWKDKGNQVVTSWKNKGFSRDAR 240

Query: 241 GMVDGSWRDKV-HQGGATSWKTKGFASDTRNNTRGWNQKQR-ACNFDHYNRPWSNSYNRN 300
            MVD +WR  V +Q GA +WKTKGFASD RNN+  W++ Q+ A NFDHYNRP +++YNRN
Sbjct: 241 SMVDNAWRANVQYQDGAATWKTKGFASDARNNS--WSRHQQGASNFDHYNRPGNSNYNRN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQW-YGKRPKDAEF 332
           VRNL DR+ PN  GN+  WKY++ YGKRPKDA+F
Sbjct: 301 VRNLPDRMPPNIHGNKQEWKYKYRYGKRPKDAQF 332

BLAST of MS018721 vs. ExPASy TrEMBL
Match: A0A1S3CQ16 (uncharacterized protein LOC103503387 OS=Cucumis melo OX=3656 GN=LOC103503387 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.5e-133
Identity = 233/364 (64.01%), Postives = 267/364 (73.35%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSAIRNYDHDPPLE W+DGIPLWEKKFC EIGCVPWGKI+DS
Sbjct: 1   MGNWRERPRRNFRYQKPPWSAIRNYDHDPPLERWRDGIPLWEKKFCKEIGCVPWGKIIDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH N+ NW+DSACEEAFHNAKR +WA+ NGH+ DIYLPDPDKYIE+IDW+P++DS
Sbjct: 61  KNFIYCHTNVANWDDSACEEAFHNAKRRYWAEINGHQSDIYLPDPDKYIEQIDWSPDMDS 120

Query: 121 EMIEELDWAYFAPNVTESD------------------------------DLLEGKNKRTK 180
           +MIEELDWAY  PN+ + +                              D LE KNKRT+
Sbjct: 121 KMIEELDWAYHTPNIKQREEVDWSYHPPIMKQHEELDWAYHTPNMKQRKDWLECKNKRTR 180

Query: 181 NSSSVWAEGHIEDSGYVGNAWEHGIQCIANTGEGWNQRNLSDSGKANNDGNPWDSSVTQV 240
           +SSSVW EGHIE  G+  N W H  Q   NTG+G +Q N SDSGK NNDGNPWDSS+ Q 
Sbjct: 181 DSSSVWKEGHIEGPGHGANPWGHDNQLTDNTGQGCSQWNFSDSGKVNNDGNPWDSSIYQG 240

Query: 241 NGGIVDSAWRDKVQQGDTSWNTKGFSSDARGMVDGSWRDKVHQGGATSWKTKGFASDTRN 300
           N G+VDSAW+ K  Q  TSW  K F+SDARG+VD +WRDK+HQGG  SWKTKGFASD RN
Sbjct: 241 NKGMVDSAWKVKKNQVVTSWKNKEFASDARGVVDNAWRDKLHQGGTASWKTKGFASDARN 300

Query: 301 N--TRGWNQKQRACNFDHYNRPWSNSYNRNVRNLTDRIQPNNQGNEHGWKYQW-YGKRPK 332
           N  +R W+    + NFDHYN P +NSYNRNV +L DR +PN  GN   WKYQ+ YGKRPK
Sbjct: 301 NSSSRHWHLLGDS-NFDHYNGPGNNSYNRNVWHLPDRTRPNIHGNNQDWKYQYRYGKRPK 360

BLAST of MS018721 vs. ExPASy TrEMBL
Match: A0A0A0KNE4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G440120 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 223/333 (66.97%), Postives = 261/333 (78.38%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MGNWRERPRR FR QKPPWSAIRNYDHDPPLE W+DGIPLWEKKFC EIGCVPWGKIVDS
Sbjct: 1   MGNWRERPRRNFRYQKPPWSAIRNYDHDPPLEHWRDGIPLWEKKFCAEIGCVPWGKIVDS 60

Query: 61  KKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEIDS 120
           K FIYCH+N+V W+DSACEEAFHNAKR +WA+ NGH+ DI+LPDPDK+IE+IDW+P++DS
Sbjct: 61  KNFIYCHSNVVKWDDSACEEAFHNAKRRYWAEINGHQSDIHLPDPDKHIEQIDWSPDMDS 120

Query: 121 EMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSGYVGNAWEHGIQCIAN 180
           +MIEELDWAY+ PN+ + DD LE KNKRT+NS+SVW EGHIE  G+ GN W H  Q    
Sbjct: 121 KMIEELDWAYYTPNMKQRDDWLECKNKRTRNSNSVWTEGHIEGPGHEGNPWGHDNQLTDK 180

Query: 181 TGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSSDAR 240
           TG+G  + N SDSG  NNDGNPWDSS+ Q N G+VDS W+ +  Q  TSW  K F+S+AR
Sbjct: 181 TGQGCRRWNFSDSGNVNNDGNPWDSSINQGNKGMVDSIWKVEKNQVATSWKNKEFASNAR 240

Query: 241 GMVDGSWRDKVHQGGATSWKTKGFASDTRNNT--RGWNQKQRACNFDHYNRPWSNSYNRN 300
           G+VD + +DK HQGG  SWKT+GFASD RNN+  R W+    + NFDHYNRP ++SY  N
Sbjct: 241 GVVDNARKDKQHQGGTASWKTEGFASDARNNSSRRQWHLLGNS-NFDHYNRPGNSSYIHN 300

Query: 301 VRNLTDRIQPNNQGNEHGWKYQWYGKRPKDAEF 332
           VR+L DR QPN   N    KYQ YGKRPKD EF
Sbjct: 301 VRHLPDRTQPNIHRNNQDLKYQ-YGKRPKDTEF 331

BLAST of MS018721 vs. TAIR 10
Match: AT3G51940.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G03990.1); Has 215 Blast hits to 164 proteins in 38 species: Archae - 0; Bacteria - 35; Metazoa - 16; Fungi - 18; Plants - 121; Viruses - 0; Other Eukaryotes - 25 (source: NCBI BLink). )

HSP 1 Score: 151.4 bits (381), Expect = 1.3e-36
Identity = 116/385 (30.13%), Postives = 174/385 (45.19%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHDPPLEFWKDGIPLWEKKFCTEIGCVPWGKIVDS 60
           MG W  R R   R+    W + R            DGIP+WEK+FC  IG VPW K+V++
Sbjct: 1   MGKWNHRSRHHRRRSPERWYSGRQSSSSS-----DDGIPVWEKRFCEVIGSVPWQKVVEA 60

Query: 61  KKFIYCHN-NIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYIEKIDWNPEID 120
           K F   +N N++ W+DSACE+ FHN K+ FW+Q NG  CD+ +PDPD YI ++DW+  +D
Sbjct: 61  KDFKSWYNGNVITWDDSACEDTFHNEKKRFWSQVNGLHCDVSIPDPDLYISEVDWDTFVD 120

Query: 121 SEMIEELDWAYFAP---------------NVTESDDLLEGKNKRT--KNSSSVWAEGHIE 180
            E+I +L+ AYFAP               N +  D + E +   T  KNS  +   G  +
Sbjct: 121 PELIRDLEKAYFAPPDDVNIGFKRGRGDKNWSGCDTVPEARMLETPWKNSDDIIETGK-K 180

Query: 181 DSGY---VGNAWEHGIQCI---AN--------TGEGWNQR------NLSD----SGKANN 240
            SG+    G++WE    C+   AN        T E W +        ++D    SG+  +
Sbjct: 181 SSGWNLTEGSSWEAKPCCVNEKANDTASGGCLTTEEWRENQWIAKDRVNDSWEYSGQGKD 240

Query: 241 DG---------------------NPWDSSVTQVNGGIVDSAWRDKVQQGDTSWNTKGFSS 300
           DG                     NPW++  + +     D+ W     +G   W  +G+++
Sbjct: 241 DGWDKSGHQNKKVKGSEEYKKIDNPWEAQPSCIKETAKDTTWGGCSGEG---WEDRGWNN 300

Query: 301 DARGMVDGSW--RDKVHQG-GATSWKTKGFASDTRNNTRGWNQKQRACNFDHYNRPWSNS 319
           D+ G   G W  RD  +QG     W+ KG++ D R        + + CN      PW   
Sbjct: 301 DSWG--SGGWDNRDLGNQGMEMKEWRGKGYSRDFR--------EPKGCN------PWKGG 354

BLAST of MS018721 vs. TAIR 10
Match: AT5G03990.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G51940.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 125.9 bits (315), Expect = 5.9e-29
Identity = 102/340 (30.00%), Postives = 149/340 (43.82%), Query Frame = 0

Query: 1   MGNWRERPRRIFRKQKPPWSAIRNYDHD-----------PPLEFWKDGIPLWEKKFCTEI 60
           M NW        R+QKP  +   NY              PPL   K  +P WEK FC  I
Sbjct: 1   MSNW--------RRQKPRNNNSNNYSRQRGTTMTQSSSKPPLANCKISVPAWEKDFCAVI 60

Query: 61  GCVPWGKIVDSKKFIYCHNNIVNWNDSACEEAFHNAKRHFWAQTNGHRCDIYLPDPDKYI 120
           G VPW K+V++K+F++ ++ +V W+DSA E+AF NAK  FWA+ NG  CD+ LPDPD YI
Sbjct: 61  GSVPWWKVVEAKRFMHIYDRVVQWDDSAGEDAFKNAKSRFWAEINGLTCDLSLPDPDVYI 120

Query: 121 EKIDWNPEIDSEMIEELDWAYFAPNVTESDDLLEGKNKRTKNSSSVWAEGHIEDSG-YVG 180
           + +DW+ E+D+E+I +L+                G +  T+    V     +  SG Y G
Sbjct: 121 DDVDWDAEVDNELILDLE---------------RGPDPLTEEQEHVVILDALVLSGQYGG 180

Query: 181 NAWEHGIQCIANTGEGWNQRNLSDSGKANNDGNPWDSSVTQVNGGIVDSAWRDKVQQG-- 240
             W          G GW      D+   N +          V  G  +++W D+   G  
Sbjct: 181 LGW----------GTGW-----GDAEGINEE---------NVGKGKPENSWGDQKYDGWN 240

Query: 241 DTSWNTKGFSSDARGMVDGSWRDKVHQGGATSWKTKGFASDTRNNTRGWNQKQRACNFDH 300
           + SW  K  +         +W    +     SW       D  NN +     Q+ CN   
Sbjct: 241 EDSWGIKEKTE--------TWDHNNNNTNTDSW-------DHNNNFKAETWDQKNCN--- 266

Query: 301 YNRPWSNSYN-RNVRNLTDRIQPNNQGNEH-GWKYQWYGK 325
                +NS+N + V N   R    +QG E+ GW+ +  G+
Sbjct: 301 -----NNSFNYKKVENWNGR----DQGRENRGWRKRGEGR 266