MS015282 (gene) Bitter gourd (TR) v1

Overview
NameMS015282
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein of unknown function (DUF1262)
Locationscaffold2: 2273187 .. 2275577 (+)
RNA-Seq ExpressionMS015282
SyntenyMS015282
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGTGACAAGGCCTCTTTCGATGTACAGAAATGAGGGCGCTACTTCGGCAGCGCCGGTGCCGGCGGCGTTGGTGGAGGGTCCAAATACGGGGGTTCTGGTGATAGAAGATGAAGCGGCGGAATCGAGATGGCTTTTTGGGTTGCTTAAGAGGAAGTCGGTGAAGGCGCCGCCCTTCCCTCAGAACAAGATAATGGAGCTTCGCTATACCATAAACTCAGGGGAGCACCAGCACACCGATTACTTCTACGCGCTGCTCATTCCCGTTATCAATCGCCCCTTGTCTTCCAACAAATATTACGTCATCAAATCTCGCGGCAAAACAAAAGGGTATGCGCCTCCCTCTCATCTTCATTTCTTCTCCTTTTCCTTTTTGCGCGTCGTCGGCCATCCCATTCCGTTGGGGATTTTTCCTCATCTCCCCTTCCGTTCCAATATATATATTTTACTTTCTTTTAGAAAGATTTAGGAAGAAATTAGGCTACTATACAAAGAAAAATTGGACTTCGGGCGCTTTTTTGGTTAAAAATTAAAAAAATATATATAATAATTAATATATATTTATATTAATAATATATAAAAATATACAATATAAATAAAATATTAAATCCCGTCCTGATCGGAAACTCGATTAGGGTCTCCAATTTGATCCCGTCTCCAATCAAATCGGGAATTTCCCACCCAATCAGGTCGGGTGGTATATATAGGGATTAAGTTAGGTTAAAATATACCTCATTATTTAGTATCTGTTATGGAAACTTTTATCCGTCCAGAAATTCTAATTCCTTGATAAACAAAACTTGCATACACGTGTACCTACATGTGTTTAGTTGGGAAGCAAATAAACAAATAGTTGGAACTGGAAGTAATATTTATCAAATTATTTTTAAGAATAAAAAATTAATAACGAAATAGTTATGTCTTTTATTTAATTAGATTTGGGTATTTTGTTGTTAAGTCTAAACGTGTTTTAATTTGTGCTATTTCGCGATGAAGTTGAATCCTAGGTCTGTGATTTGAGTTTAGCCTTTCTTTCTTTGACATGGTCACATGACGATTCAGCGTTTGGGTTTGTTAAATTTTGCTTGATTTATGTGTCAGTCATCCACAAATAATAATTTTTAGTTTATTTTTCAGCAGCCATTATAAATCATATTAAATATTTGTCGGTCCCTGATATTTTCCACATATTTATGTATTTGACGGTATAAATCTGACATTTTCACCGCTTGAGATAAAATATTATAAATATTAAAAAAATGTCACTAATATATACATAATATTAATATTAATCATTTTTTTAATAAATAAATATTTTATTATAAATAAATATTATAAATAATAATCATTATAAATAAATAAATATTTATGTATTAATTTAAATATTTTTTACTTTCCGAAGTATTTATCAATATTTTATCAATATTTTCATGAAATTGGGATTTCGACATGATCCGATCTCGATCCCGACCCTTATAGCTTGTTAATATCTATAAACATAGGATTCGATGTGATAATAGTCCAAATGTATGAACCTAAACGCAGGCTAGCATGTACAAGTTCAAAGGAAGACGATGAGACCAGCTGCTGTTGCTTTGGCATCCCTGATACACCACCACAACTCTTCGACCCAACAAATGCCTACCAACAGTTCCAAATCAGTAATTATGTACAGTGTGCAGGGCCCTGTGGTTTTATGGCCAACTCCATAGCTCCTGACGGGGTTCCACCCCGTTTCCTTAGGCGCAGGGGCTGGAGAGCTTATACCAAAACCTTCAAGAACAAAAACTTGGAGCTCACCCAAGCGCTCGGCCTTGACGCCGCGCTGCGTGCCCGCCTCCCAGCTCTCAACTTCTCGCTGCCGGCCAAATCTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGGGATGGAGAGGTTGGCTCTCAGGTGAGTAACTCGCCGTACTATGAAATGACCCTCCAACAGAGTTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTGTCTGTGGAAAAGGAAGTGATTTTGATTGCGGGTCAGCGGACCGATGGTAGAGAAGTTGGTGATGGGGTTGTGTGGTTCGGGTCGTGGGGGGTTGGGCTGAGCTTGGCGATTGTGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAATTTGGAAAAGAAAAGGAAAAGAAATATGTGAAGGTGAAGAGAAGAGAGGAGTATGGAGGGGTAGGAGAGTGGAAGAGATTTGGGTGTTATGTGTTAATTGAGAGGTTTGTGCTCAAGAGAATGGATGGAAGCTTAGTTCTTACTTGGGAATTTAAGCACACTCATCAAATTAGGACCAAATGGGAG

mRNA sequence

ATGTACGTGACAAGGCCTCTTTCGATGTACAGAAATGAGGGCGCTACTTCGGCAGCGCCGGTGCCGGCGGCGTTGGTGGAGGGTCCAAATACGGGGGTTCTGGTGATAGAAGATGAAGCGGCGGAATCGAGATGGCTTTTTGGGTTGCTTAAGAGGAAGTCGGTGAAGGCGCCGCCCTTCCCTCAGAACAAGATAATGGAGCTTCGCTATACCATAAACTCAGGGGAGCACCAGCACACCGATTACTTCTACGCGCTGCTCATTCCCGTTATCAATCGCCCCTTGTCTTCCAACAAATATTACGTCATCAAATCTCGCGGCAAAACAAAAGGCTTGTTAATATCTATAAACATAGGATTCGATGTGATAATAGTCCAAATGTATGAACCTAAACGCAGGCTAGCATGTACAAGTTCAAAGGAAGACGATGAGACCAGCTGCTGTTGCTTTGGCATCCCTGATACACCACCACAACTCTTCGACCCAACAAATGCCTACCAACAGTTCCAAATCAGTAATTATGTACAGTGTGCAGGGCCCTGTGGTTTTATGGCCAACTCCATAGCTCCTGACGGGGTTCCACCCCGTTTCCTTAGGCGCAGGGGCTGGAGAGCTTATACCAAAACCTTCAAGAACAAAAACTTGGAGCTCACCCAAGCGCTCGGCCTTGACGCCGCGCTGCGTGCCCGCCTCCCAGCTCTCAACTTCTCGCTGCCGGCCAAATCTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGGGATGGAGAGGTTGGCTCTCAGGTGAGTAACTCGCCGTACTATGAAATGACCCTCCAACAGAGTTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTGTCTGTGGAAAAGGAAGTGATTTTGATTGCGGGTCAGCGGACCGATGGTAGAGAAGTTGGTGATGGGGTTGTGTGGTTCGGGTCGTGGGGGGTTGGGCTGAGCTTGGCGATTGTGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAATTTGGAAAAGAAAAGGAAAAGAAATATGTGAAGGTGAAGAGAAGAGAGGAGTATGGAGGGGTAGGAGAGTGGAAGAGATTTGGGTGTTATGTGTTAATTGAGAGGTTTGTGCTCAAGAGAATGGATGGAAGCTTAGTTCTTACTTGGGAATTTAAGCACACTCATCAAATTAGGACCAAATGGGAG

Coding sequence (CDS)

ATGTACGTGACAAGGCCTCTTTCGATGTACAGAAATGAGGGCGCTACTTCGGCAGCGCCGGTGCCGGCGGCGTTGGTGGAGGGTCCAAATACGGGGGTTCTGGTGATAGAAGATGAAGCGGCGGAATCGAGATGGCTTTTTGGGTTGCTTAAGAGGAAGTCGGTGAAGGCGCCGCCCTTCCCTCAGAACAAGATAATGGAGCTTCGCTATACCATAAACTCAGGGGAGCACCAGCACACCGATTACTTCTACGCGCTGCTCATTCCCGTTATCAATCGCCCCTTGTCTTCCAACAAATATTACGTCATCAAATCTCGCGGCAAAACAAAAGGCTTGTTAATATCTATAAACATAGGATTCGATGTGATAATAGTCCAAATGTATGAACCTAAACGCAGGCTAGCATGTACAAGTTCAAAGGAAGACGATGAGACCAGCTGCTGTTGCTTTGGCATCCCTGATACACCACCACAACTCTTCGACCCAACAAATGCCTACCAACAGTTCCAAATCAGTAATTATGTACAGTGTGCAGGGCCCTGTGGTTTTATGGCCAACTCCATAGCTCCTGACGGGGTTCCACCCCGTTTCCTTAGGCGCAGGGGCTGGAGAGCTTATACCAAAACCTTCAAGAACAAAAACTTGGAGCTCACCCAAGCGCTCGGCCTTGACGCCGCGCTGCGTGCCCGCCTCCCAGCTCTCAACTTCTCGCTGCCGGCCAAATCTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGGGATGGAGAGGTTGGCTCTCAGGTGAGTAACTCGCCGTACTATGAAATGACCCTCCAACAGAGTTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTGTCTGTGGAAAAGGAAGTGATTTTGATTGCGGGTCAGCGGACCGATGGTAGAGAAGTTGGTGATGGGGTTGTGTGGTTCGGGTCGTGGGGGGTTGGGCTGAGCTTGGCGATTGTGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAATTTGGAAAAGAAAAGGAAAAGAAATATGTGAAGGTGAAGAGAAGAGAGGAGTATGGAGGGGTAGGAGAGTGGAAGAGATTTGGGTGTTATGTGTTAATTGAGAGGTTTGTGCTCAAGAGAATGGATGGAAGCTTAGTTCTTACTTGGGAATTTAAGCACACTCATCAAATTAGGACCAAATGGGAG

Protein sequence

MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGFDVIIVQMYEPKRRLACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE
Homology
BLAST of MS015282 vs. NCBI nr
Match: XP_022149086.1 (uncharacterized protein LOC111017584 [Momordica charantia])

HSP 1 Score: 804.7 bits (2077), Expect = 3.8e-229
Identity = 391/414 (94.44%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF
Sbjct: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKG         
Sbjct: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP 180
                        LACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP
Sbjct: 121 -------------LACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP 180

Query: 181 CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLPA 240
           CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRA LPALNFSLPA
Sbjct: 181 CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRAHLPALNFSLPA 240

Query: 241 KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD 300
           KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD
Sbjct: 241 KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD 300

Query: 301 VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK 360
           VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK
Sbjct: 301 VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK 360

Query: 361 YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
           YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE
Sbjct: 361 YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 392

BLAST of MS015282 vs. NCBI nr
Match: XP_022149112.1 (uncharacterized protein LOC111017603 [Momordica charantia])

HSP 1 Score: 551.6 bits (1420), Expect = 5.8e-153
Identity = 278/419 (66.35%), Postives = 319/419 (76.13%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIED--EAAESRWLFGLLKRK-SVKA 60
           MYVTRPLS+YRN  +  ++P P    EGPN+G+LV ED  E AESRW FG+ K+K SVK 
Sbjct: 1   MYVTRPLSLYRNSPSWLSSPPP----EGPNSGILVFEDEEEGAESRWFFGVFKKKDSVKV 60

Query: 61  PPFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISIN 120
           PP PQN+I+ L +  ++GE++++D  YALL+PV+N+PLSSN+YYVI SRG  KG      
Sbjct: 61  PPLPQNRILRLTHAADAGEYEYSDSLYALLVPVLNQPLSSNQYYVIDSRGTAKG------ 120

Query: 121 IGFDVIIVQMYEPKRRLACTSSKEDDET--SCCCFGIPDTPPQLFDPTNAYQQFQISNYV 180
                           LACTSSKED+ +  S   + I D P QL DP N YQQFQISNY+
Sbjct: 121 ----------------LACTSSKEDNTSRRSRFFYRIHDAPAQLLDPKNTYQQFQISNYI 180

Query: 181 QCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALN 240
            C  P GF++ S+APDGVPP  LRR+GWRAY +   N N   T+ALGLDAALRARLP L 
Sbjct: 181 YCGKPNGFISKSVAPDGVPPELLRRKGWRAYPEPLNNNNSLPTEALGLDAALRARLPDLA 240

Query: 241 FSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGER 300
           F       +PVVVGKWYCPFIFVRDG V SQ+SNSPYYEMTLQQ+WEEIFGCG+LGGG R
Sbjct: 241 F-----PPNPVVVGKWYCPFIFVRDGAVESQMSNSPYYEMTLQQNWEEIFGCGSLGGGGR 300

Query: 301 GVDFDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGK 360
            VDFDVSVE+EVI IAGQ   GR  GDGV+WFGS GVGLSLAIVERVKWEEERAGFEFGK
Sbjct: 301 VVDFDVSVEREVISIAGQAAGGRNAGDGVMWFGSSGVGLSLAIVERVKWEEERAGFEFGK 360

Query: 361 EKEKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
           EKEKKYVKVKRREE+   GEW+RFGCYVL+ERFVLKRMDGSLVLTWEF+HTHQIRTKWE
Sbjct: 361 EKEKKYVKVKRREEFKERGEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWE 388

BLAST of MS015282 vs. NCBI nr
Match: XP_022945636.1 (uncharacterized protein LOC111449821 [Cucurbita moschata])

HSP 1 Score: 510.0 bits (1312), Expect = 1.9e-140
Identity = 256/416 (61.54%), Postives = 302/416 (72.60%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLS YRN  A +AA       EGPNTG+LVIEDEA+ES+WLFGLLKR+SVKA PF
Sbjct: 1   MYVTRPLSAYRNSPAAAAAAACEVEPEGPNTGILVIEDEASESKWLFGLLKRRSVKALPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN IMEL YT N GEHQ  D   ALLIPV+N+P SSN+YYVI+SRG++KG         
Sbjct: 61  PQNMIMELHYTRNEGEHQRIDRLNALLIPVLNQPSSSNQYYVIRSRGRSKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIP-DTPPQLFDPTNAYQQFQISNYVQCAG 180
                        LACTSSKE+D TSC CF    DT PQLFDPTNAYQQFQ+   + C G
Sbjct: 121 -------------LACTSSKEEDMTSCGCFTFTHDTTPQLFDPTNAYQQFQLDKNMSCLG 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLP 240
           PCGFM++S+APDGVPPRFLR +GWRAYTK    KN + TQALG+D ALR RLP LN    
Sbjct: 181 PCGFMSHSMAPDGVPPRFLRHKGWRAYTKPL--KNFKPTQALGVDVALRGRLPELNLK-- 240

Query: 241 AKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGG-GERGVD 300
                PVVVGKWYCPFIF+R+GEVG Q+ +SPYYEMTL+Q WEE+FGC N GG    GV+
Sbjct: 241 -----PVVVGKWYCPFIFIREGEVGDQMRSSPYYEMTLEQHWEEVFGCYNEGGLVGNGVE 300

Query: 301 FDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKE 360
            DV V+++ + + G   +   V  GVVWFG   VG+S A+VERVKWEEER GF++G  +E
Sbjct: 301 VDVYVKRQGVFVGGVAAERVVVDGGVVWFGEREVGVSTAVVERVKWEEERGGFKWGGHEE 360

Query: 361 KKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
            +  +V RRE + G+G W+RFGCY L+ERFVLKRMDGS+VLTW+F HTHQIR KWE
Sbjct: 361 VE--RVVRREAFEGLGVWRRFGCYALVERFVLKRMDGSVVLTWDFTHTHQIRIKWE 383

BLAST of MS015282 vs. NCBI nr
Match: XP_023542039.1 (uncharacterized protein LOC111802013 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.1 bits (1307), Expect = 7.3e-140
Identity = 258/416 (62.02%), Postives = 301/416 (72.36%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLS YRN  A +A  V     EGPNTGVLVIEDEA+ES+WLFGLLKR+SVKA PF
Sbjct: 1   MYVTRPLSAYRNSPAAAACEVEP---EGPNTGVLVIEDEASESKWLFGLLKRRSVKALPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN IMELRYT N GEHQ  D   ALLIPV+N+P SSN+YYVI+SRG++KG         
Sbjct: 61  PQNMIMELRYTRNEGEHQRIDRLNALLIPVLNQPSSSNQYYVIRSRGRSKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIP-DTPPQLFDPTNAYQQFQISNYVQCAG 180
                        LACTSSKE+D TSC CF    DT PQLFDPTNAYQQFQ+   + C G
Sbjct: 121 -------------LACTSSKEEDMTSCGCFTFTHDTTPQLFDPTNAYQQFQLDKNMSCLG 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLP 240
           PCGFM++S+APDGVPPRFLR +GWRAYTK    KN + TQALGLD ALR RLP LN    
Sbjct: 181 PCGFMSHSMAPDGVPPRFLRHKGWRAYTKPL--KNFKPTQALGLDVALRGRLPGLNLK-- 240

Query: 241 AKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGG-GERGVD 300
                PVVVGKWYCPFIFVR+GEVG Q+ + PYYEMTL+Q WEE+FGC N GG    GV+
Sbjct: 241 -----PVVVGKWYCPFIFVREGEVGDQMRSWPYYEMTLEQHWEEVFGCYNEGGLVGNGVE 300

Query: 301 FDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKE 360
            DV V ++ + + G   +   V  GV+WFG   VG+S A+VERVKWEEER GF++G  +E
Sbjct: 301 VDVCVRRQGVFVGGVAAERVVVDGGVMWFGEREVGVSTAVVERVKWEEERGGFKWGAHEE 360

Query: 361 KKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
            +  +V RRE + G+G W+RFGCY L+ERFVLKRMDGS+VLTW+F HTHQIR KWE
Sbjct: 361 VE--RVVRREAFEGLGVWRRFGCYALVERFVLKRMDGSVVLTWDFTHTHQIRIKWE 380

BLAST of MS015282 vs. NCBI nr
Match: KAG6574031.1 (hypothetical protein SDJN03_27918, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 507.3 bits (1305), Expect = 1.3e-139
Identity = 256/416 (61.54%), Postives = 301/416 (72.36%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLS YRN  A +A  V     EGPNTG+LVIEDEA+ES+WLFGLLKR+SVKA PF
Sbjct: 1   MYVTRPLSAYRNSPAAAACEVEP---EGPNTGILVIEDEASESKWLFGLLKRRSVKALPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN IMELRYT N GEHQ  D   ALLIPV+N+P SSN+YYVI+SRG++KG         
Sbjct: 61  PQNMIMELRYTRNEGEHQRIDRLNALLIPVLNQPSSSNQYYVIRSRGRSKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIP-DTPPQLFDPTNAYQQFQISNYVQCAG 180
                        LACTSSKE+D TSC CF    DT PQLFDPTNAYQQFQ+   + C G
Sbjct: 121 -------------LACTSSKEEDMTSCGCFTFTHDTTPQLFDPTNAYQQFQLDKNMSCLG 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLP 240
           PCGFM++S+APDGVPPRFLR +GWRAYTK    KN + TQALG+D ALR RLP LN    
Sbjct: 181 PCGFMSHSMAPDGVPPRFLRHKGWRAYTKPL--KNFKPTQALGVDVALRGRLPELNLK-- 240

Query: 241 AKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGG-GERGVD 300
                P VVGKWYCPFIF+R+GEVG Q+ +SPYYEMTL+Q WEE+FGC N GG    GV+
Sbjct: 241 -----PTVVGKWYCPFIFIREGEVGDQMRSSPYYEMTLEQHWEEVFGCYNEGGLVGNGVE 300

Query: 301 FDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKE 360
            DV V+++ + + G   +   V  GVVWFG   VG+S A+VERVKWEEER GF +G  +E
Sbjct: 301 VDVYVKRQGVFVGGVAAERVVVDGGVVWFGEREVGVSTAVVERVKWEEERGGFRWGGHEE 360

Query: 361 KKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
            +  +V RRE + G+G W+RFGCY L+ERFVLKRMDGS+VLTW+F HTHQIR KWE
Sbjct: 361 VE--RVVRREAFEGLGVWRRFGCYALVERFVLKRMDGSVVLTWDFTHTHQIRIKWE 380

BLAST of MS015282 vs. ExPASy TrEMBL
Match: A0A6J1D7B1 (uncharacterized protein LOC111017584 OS=Momordica charantia OX=3673 GN=LOC111017584 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.8e-229
Identity = 391/414 (94.44%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF
Sbjct: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKG         
Sbjct: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP 180
                        LACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP
Sbjct: 121 -------------LACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCAGP 180

Query: 181 CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLPA 240
           CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRA LPALNFSLPA
Sbjct: 181 CGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRAHLPALNFSLPA 240

Query: 241 KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD 300
           KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD
Sbjct: 241 KSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFD 300

Query: 301 VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK 360
           VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK
Sbjct: 301 VSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKK 360

Query: 361 YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
           YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE
Sbjct: 361 YVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 392

BLAST of MS015282 vs. ExPASy TrEMBL
Match: A0A6J1D6Y4 (uncharacterized protein LOC111017603 OS=Momordica charantia OX=3673 GN=LOC111017603 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.8e-153
Identity = 278/419 (66.35%), Postives = 319/419 (76.13%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIED--EAAESRWLFGLLKRK-SVKA 60
           MYVTRPLS+YRN  +  ++P P    EGPN+G+LV ED  E AESRW FG+ K+K SVK 
Sbjct: 1   MYVTRPLSLYRNSPSWLSSPPP----EGPNSGILVFEDEEEGAESRWFFGVFKKKDSVKV 60

Query: 61  PPFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISIN 120
           PP PQN+I+ L +  ++GE++++D  YALL+PV+N+PLSSN+YYVI SRG  KG      
Sbjct: 61  PPLPQNRILRLTHAADAGEYEYSDSLYALLVPVLNQPLSSNQYYVIDSRGTAKG------ 120

Query: 121 IGFDVIIVQMYEPKRRLACTSSKEDDET--SCCCFGIPDTPPQLFDPTNAYQQFQISNYV 180
                           LACTSSKED+ +  S   + I D P QL DP N YQQFQISNY+
Sbjct: 121 ----------------LACTSSKEDNTSRRSRFFYRIHDAPAQLLDPKNTYQQFQISNYI 180

Query: 181 QCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALN 240
            C  P GF++ S+APDGVPP  LRR+GWRAY +   N N   T+ALGLDAALRARLP L 
Sbjct: 181 YCGKPNGFISKSVAPDGVPPELLRRKGWRAYPEPLNNNNSLPTEALGLDAALRARLPDLA 240

Query: 241 FSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGER 300
           F       +PVVVGKWYCPFIFVRDG V SQ+SNSPYYEMTLQQ+WEEIFGCG+LGGG R
Sbjct: 241 F-----PPNPVVVGKWYCPFIFVRDGAVESQMSNSPYYEMTLQQNWEEIFGCGSLGGGGR 300

Query: 301 GVDFDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGK 360
            VDFDVSVE+EVI IAGQ   GR  GDGV+WFGS GVGLSLAIVERVKWEEERAGFEFGK
Sbjct: 301 VVDFDVSVEREVISIAGQAAGGRNAGDGVMWFGSSGVGLSLAIVERVKWEEERAGFEFGK 360

Query: 361 EKEKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
           EKEKKYVKVKRREE+   GEW+RFGCYVL+ERFVLKRMDGSLVLTWEF+HTHQIRTKWE
Sbjct: 361 EKEKKYVKVKRREEFKERGEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWE 388

BLAST of MS015282 vs. ExPASy TrEMBL
Match: A0A6J1G1G0 (uncharacterized protein LOC111449821 OS=Cucurbita moschata OX=3662 GN=LOC111449821 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 9.4e-141
Identity = 256/416 (61.54%), Postives = 302/416 (72.60%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLS YRN  A +AA       EGPNTG+LVIEDEA+ES+WLFGLLKR+SVKA PF
Sbjct: 1   MYVTRPLSAYRNSPAAAAAAACEVEPEGPNTGILVIEDEASESKWLFGLLKRRSVKALPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN IMEL YT N GEHQ  D   ALLIPV+N+P SSN+YYVI+SRG++KG         
Sbjct: 61  PQNMIMELHYTRNEGEHQRIDRLNALLIPVLNQPSSSNQYYVIRSRGRSKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIP-DTPPQLFDPTNAYQQFQISNYVQCAG 180
                        LACTSSKE+D TSC CF    DT PQLFDPTNAYQQFQ+   + C G
Sbjct: 121 -------------LACTSSKEEDMTSCGCFTFTHDTTPQLFDPTNAYQQFQLDKNMSCLG 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLP 240
           PCGFM++S+APDGVPPRFLR +GWRAYTK    KN + TQALG+D ALR RLP LN    
Sbjct: 181 PCGFMSHSMAPDGVPPRFLRHKGWRAYTKPL--KNFKPTQALGVDVALRGRLPELNLK-- 240

Query: 241 AKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGG-GERGVD 300
                PVVVGKWYCPFIF+R+GEVG Q+ +SPYYEMTL+Q WEE+FGC N GG    GV+
Sbjct: 241 -----PVVVGKWYCPFIFIREGEVGDQMRSSPYYEMTLEQHWEEVFGCYNEGGLVGNGVE 300

Query: 301 FDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKE 360
            DV V+++ + + G   +   V  GVVWFG   VG+S A+VERVKWEEER GF++G  +E
Sbjct: 301 VDVYVKRQGVFVGGVAAERVVVDGGVVWFGEREVGVSTAVVERVKWEEERGGFKWGGHEE 360

Query: 361 KKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
            +  +V RRE + G+G W+RFGCY L+ERFVLKRMDGS+VLTW+F HTHQIR KWE
Sbjct: 361 VE--RVVRREAFEGLGVWRRFGCYALVERFVLKRMDGSVVLTWDFTHTHQIRIKWE 383

BLAST of MS015282 vs. ExPASy TrEMBL
Match: A0A6J1HWZ9 (uncharacterized protein LOC111467621 OS=Cucurbita maxima OX=3661 GN=LOC111467621 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 4.3e-138
Identity = 253/416 (60.82%), Postives = 301/416 (72.36%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTRPLS YRN  A +    P    EGPNTGVLVI+DEA+ES+WLFGLLKR+SVK  PF
Sbjct: 1   MYVTRPLSAYRNSPAAACDVEP----EGPNTGVLVIKDEASESKWLFGLLKRRSVKVLPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN IMELRYT N GEHQ  D   ALLIPV+N+PLSSN+YYVI+SRG++KG         
Sbjct: 61  PQNMIMELRYTRNEGEHQRIDRLNALLIPVLNQPLSSNQYYVIRSRGRSKG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFGIP-DTPPQLFDPTNAYQQFQISNYVQCAG 180
                        LACTSSKE+D TSC CF    DT PQLFDPTNAYQQFQ+   + C G
Sbjct: 121 -------------LACTSSKEEDLTSCSCFTFAHDTTPQLFDPTNAYQQFQLDKNMSCLG 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSLP 240
           PCGFM++S+APDGV PRFLR +GWRAYTK    KN E TQA GLD ALR RLP LN    
Sbjct: 181 PCGFMSHSMAPDGVSPRFLRHKGWRAYTKPL--KNFEPTQAHGLDVALRGRLPELNLK-- 240

Query: 241 AKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGG-GERGVD 300
                PVVVGKWYCPFIF+R+GEV +Q+ +SPYYEMTL+Q+WEE+FGC N GG    GV+
Sbjct: 241 -----PVVVGKWYCPFIFIREGEVSAQMRSSPYYEMTLEQNWEEVFGCYNDGGLVGNGVE 300

Query: 301 FDVSVEKEVILIAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKE 360
            DV V+++ + + G   +   V  GV+WFG   VG+S A+VERVKWEEER GF++G  +E
Sbjct: 301 VDVCVKRQGVFVGGVAAERVVVDGGVMWFGEREVGVSTAVVERVKWEEERGGFKWGGNEE 360

Query: 361 KKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
            +  +V RRE + G+G W+RFGCY L+ERFVLKRMDGS+VLTW+F HTHQI+ KWE
Sbjct: 361 VE--RVVRREAFEGLGVWRRFGCYALVERFVLKRMDGSVVLTWDFTHTHQIKIKWE 379

BLAST of MS015282 vs. ExPASy TrEMBL
Match: A0A6J1G0V4 (uncharacterized protein LOC111449654 OS=Cucurbita moschata OX=3662 GN=LOC111449654 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 3.3e-130
Identity = 241/417 (57.79%), Postives = 299/417 (71.70%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIED-EAAESRWLFGLLKRK-SVKAP 60
           MYVTRPLS+YRN  ++ ++  P    EGPN+G+LVI+D E AE+RW  GL K K SVK  
Sbjct: 1   MYVTRPLSLYRNSPSSLSSAPP----EGPNSGILVIQDEEPAETRWCCGLFKTKESVKEL 60

Query: 61  PFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINI 120
           PFPQNKI+ L +   +GE ++++   A+LIPV+N PLSSN+YY+I S G  KG       
Sbjct: 61  PFPQNKILRLTHAAEAGEIEYSESVRAVLIPVLNLPLSSNQYYIINSHGTRKG------- 120

Query: 121 GFDVIIVQMYEPKRRLACTSSKEDDETSCCCFGIPDTPPQLFDPTNAYQQFQISNYVQCA 180
                          LACTSSKE++ +  CC+ + D PPQLFDP NAYQQFQIS+Y+ C 
Sbjct: 121 ---------------LACTSSKEEETSGRCCYSVLDPPPQLFDPKNAYQQFQISDYIYCG 180

Query: 181 GPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSL 240
           GP G++  S+APDGVPP+ L R+G RAYT+    KN E T+ALGL+ +LR RLP +    
Sbjct: 181 GPNGYITKSMAPDGVPPKRLSRKGLRAYTQPL--KNFEPTEALGLNPSLRGRLPEI---- 240

Query: 241 PAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVD 300
             KSSDPVVVGKWYCPFIF+R+G+V SQ+SNSPYYEMTL++ W EIFGC + G G  GVD
Sbjct: 241 --KSSDPVVVGKWYCPFIFIREGDVSSQMSNSPYYEMTLRRDWFEIFGCESSGEGNNGVD 300

Query: 301 FDVSVEKEVILIAGQRTDGREV-GDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEK 360
            DV VE+EV  +AG     R   GDG VWF + GVGLS AIVERVKWEE R GF + +E 
Sbjct: 301 VDVYVEREVASVAGGAAARRRYGGDGFVWFEAAGVGLSTAIVERVKWEEGREGFGWVEEG 360

Query: 361 EKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKWE 415
           E+K ++V+RREE  GVG W+RFGCYVL+ERFVLKRMDGS+VLTWEF+HTHQ+ TKWE
Sbjct: 361 EEKKIRVRRREELKGVGIWRRFGCYVLVERFVLKRMDGSVVLTWEFRHTHQLTTKWE 383

BLAST of MS015282 vs. TAIR 10
Match: AT1G13520.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 292.7 bits (748), Expect = 4.5e-79
Identity = 169/427 (39.58%), Postives = 239/427 (55.97%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTR LS Y+     +++ +  +L EGPN+GVLVI+DE ++    FG      +K  PF
Sbjct: 1   MYVTRHLSEYQR----NSSDLTRSLPEGPNSGVLVIQDEESKPTCCFGNCYDGELKGLPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN  + + Y   +G  + + +   L IPV+++P  SN+YYVIK RGK  G         
Sbjct: 61  PQNAKLTVTYRTGTGNDRRSYHDQVLFIPVLDQPYYSNRYYVIKRRGKQSG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCC-CFG-IPDTPPQLFDPTNAYQQFQISNYVQCA 180
                         A  S+KE+D   CC CF  +P+  PQ  DP + YQQF++      +
Sbjct: 121 -------------GASASAKEEDRVPCCFCFSYVPEVKPQEADPYDIYQQFEVHQRKPSS 180

Query: 181 GPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPALNFSL 240
           G   + A S+AP+G+PP FL+R+ W       ++  L    A G++  LR++       L
Sbjct: 181 G--YYTATSVAPNGIPPEFLKRKYWTVEYSNSQDFGL-TDDAKGINTTLRSK-------L 240

Query: 241 PAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERG-- 300
           P   +  VVVGKWY PFIFV++ +   Q+ +SPYY MTL+Q WEE++ C N+   ++G  
Sbjct: 241 PNDMNTSVVVGKWYVPFIFVKERDAKDQIKSSPYYSMTLKQRWEEVYSCVNVNYNQKGGE 300

Query: 301 VDFDVSVEKEVILIAGQRTDGREV-GDGVVWFGSWG-------VGLSLAIVERVKWEEER 360
           V  DV VE +V+ + GQ T  RE  G G VWF   G       +GL   +VER+KWEEER
Sbjct: 301 VVVDVDVETQVVKLEGQETISRETSGGGFVWFSVLGDERQDKKIGLGSVVVERMKWEEER 360

Query: 361 AGFEFGKEKEKKYVKVKRREEY-GGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTH 415
            G+    E+      +KR E + GG   WK + C VLIE F LKRMDGSLVLT+EF H  
Sbjct: 361 FGWLNNGERS----NIKRSERFEGGSSHWKSYRCLVLIESFELKRMDGSLVLTYEFTHVD 387

BLAST of MS015282 vs. TAIR 10
Match: AT1G13480.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 288.9 bits (738), Expect = 6.5e-78
Identity = 170/425 (40.00%), Postives = 239/425 (56.24%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTR LS Y+ + +      P    EGPN+G++VI+DE +++   FG      +K  PF
Sbjct: 1   MYVTRRLSEYQKKPSELKLLPP----EGPNSGIMVIQDEESQATCCFGSCYEGRLKGLPF 60

Query: 61  PQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISINIGF 120
           PQN  +   YT   GEHQ T     + IPV+++PLSSN YYV++ RGK  G         
Sbjct: 61  PQNVKLNAAYTSGGGEHQTTHLTPVVFIPVLDQPLSSNLYYVLQRRGKHTG--------- 120

Query: 121 DVIIVQMYEPKRRLACTSSKEDDETSCCCFG-IPDTPPQLFDPTNAYQQFQISNYVQCAG 180
                       + + ++++E+  +SC CF  IPD  PQ  DP + YQQF+I  + + + 
Sbjct: 121 ------------KASASATEEERVSSCFCFSYIPDAKPQEADPYDIYQQFEI--HQRRSS 180

Query: 181 PCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALR--ARLPALNFS 240
              + A S+A DGVPP +L+R+ W     T  +  L    A G++  LR  + L +L  S
Sbjct: 181 SRYYSATSVASDGVPPNYLKRKDWWVSYSTSHDFGLR-DDAKGINTKLRLDSELTSLGKS 240

Query: 241 LPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGV 300
                   + +GKWY PFIFV +G+V  Q++ S +Y +TLQQ WEE+F C N+G     V
Sbjct: 241 --------IEIGKWYVPFIFVMEGDVKDQMNKSTFYSVTLQQRWEEVFFCENIGNEHFEV 300

Query: 301 DFDVSVEKEVILIAGQRTDGRE-VGDGVVWFG-------SWGVGLSLAIVERVKWEEERA 360
             DV VE E + + GQ T+ RE  GDGVVWF           +GL   +VER+KWEEER 
Sbjct: 301 VVDVDVETESVKLEGQETNLREDSGDGVVWFSVLRDEKDDKKIGLGSVVVERMKWEEERF 360

Query: 361 GFEFGKEKEKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQI 415
           G+   +  E+  +K   R E GG   WK + CYVLIE F L RMDGSLVLT+EF+H  ++
Sbjct: 361 GW-LNEAGERSNIKRSERFE-GGSSHWKSYRCYVLIESFELTRMDGSLVLTYEFRHVDKL 387

BLAST of MS015282 vs. TAIR 10
Match: AT1G13540.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 278.9 bits (712), Expect = 6.8e-75
Identity = 177/422 (41.94%), Postives = 231/422 (54.74%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESR--WLFG--LLKRKSVK 60
           MY+TR  S YR   A    P P    EGPN+G+LVI+D+ + +R    FG  L+   S+ 
Sbjct: 1   MYITRSFSEYRRNPAELTQPPP----EGPNSGILVIQDQHSRTRATSCFGSWLVVDSSLS 60

Query: 61  APPFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISI 120
             P PQN  ++L  T N G    T     + IPV+++PLSSN YY IK  GK  G     
Sbjct: 61  GLPLPQN--LKLAVTFNIGGDDST-RDPVVFIPVLDKPLSSNCYYAIKRHGKHSG----- 120

Query: 121 NIGFDVIIVQMYEPKRRLACTSSKEDDETSCC-CFG-IPDTPPQLFDPTNAYQQFQISNY 180
                             A  S+KEDD  SCC C   +P+  P+  DP + YQQF+I + 
Sbjct: 121 -----------------EASGSAKEDDIVSCCFCSSFVPEAKPKQLDPFDMYQQFKI-HQ 180

Query: 181 VQCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPAL 240
            + +  C + A S+APDGVPP FLR++ W      F++  L    A GL   LR  LP L
Sbjct: 181 KEPSSRC-YYATSVAPDGVPPWFLRKKEWTVEYSRFQDFELR-DDAKGLITELRTELPLL 240

Query: 241 NFSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGE 300
             S         VVGKWY PFIFV++ EV  Q+ NS YY MTL+Q W+E+F   N     
Sbjct: 241 GMS--------TVVGKWYVPFIFVKEREVKDQIKNSMYYSMTLEQRWKELFSDENDKSEN 300

Query: 301 RGVDFDVSVEKEVILIAGQRTDGREVGDGVVWF--GSWGVGLSLAIVERVKWEEERAGFE 360
           R V  DV VE EV+ + GQ  +     +G VWF  G   +GL   +VER+KWEEER G+ 
Sbjct: 301 RDVVVDVEVETEVVKLEGQMIERGLEANGFVWFGVGDKKIGLGSVVVERMKWEEERFGWT 360

Query: 361 FGKEKEKKYVKVKRREEYGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTK 415
            GK   ++ + VKR E+      WK + CYVLIE FVLKRMD SLVLT+EF H  +++TK
Sbjct: 361 -GKGDNERSMTVKRLEKSTDGSLWKHYHCYVLIESFVLKRMDESLVLTYEFTHVDKLKTK 381

BLAST of MS015282 vs. TAIR 10
Match: AT1G13470.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 268.9 bits (686), Expect = 7.0e-72
Identity = 169/426 (39.67%), Postives = 230/426 (53.99%), Query Frame = 0

Query: 1   MYVTRPLSMYRNEGATSAAPVPAALVEGPNTGVLVIEDEAAESRWLFGLLKRKSVKAPPF 60
           MYVTR LS Y+           + L E PN+GVL+I+DE +     FG   R ++K  PF
Sbjct: 1   MYVTRRLSEYQRN--------RSELPESPNSGVLIIQDEESRPTCCFGSCYRATLKGLPF 60

Query: 61  PQNK---IMELRYTINSGEHQHTDYF-YALLIPVINRPLSSNKYYVIKSRGKTKGLLISI 120
           PQN    + E R  +N    Q   Y    + IPV+++PLSSN+YYVIK  GK  G     
Sbjct: 61  PQNANLIVSESRIFLN----QTITYIDPVVFIPVLDQPLSSNRYYVIKRGGKHSG----- 120

Query: 121 NIGFDVIIVQMYEPKRRLACTSSKEDDETSCC-CFG-IPDTPPQLFDPTNAYQQFQISNY 180
                             A  ++KE+D   CC CF  +P+  PQ  DP + YQQFQI  +
Sbjct: 121 -----------------KASANAKEEDRVPCCFCFSYVPEAKPQEADPNDIYQQFQI--H 180

Query: 181 VQCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRARLPAL 240
            + +    + A S+AP+G PP FL+R+ W A   T ++  L+   A G+   +R+     
Sbjct: 181 QKRSSSRYYTATSVAPNGKPPEFLKRKYWNAEYSTSEDFGLK-DDAKGIYTEIRS----- 240

Query: 241 NFSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGE 300
              LP+  +  VVVGKWY PFIFV++G    Q+ +S YY M L Q WEE++ C N     
Sbjct: 241 --ELPSDFNTSVVVGKWYVPFIFVKEGNAKDQLKSSAYYSMILLQRWEEVYSCENAYNEN 300

Query: 301 RGVDFDVSVEKEVILIAGQ---RTDGREVGDGVVWF--GSWGVGLSLAIVERVKWEEERA 360
           R V  DV VE EV+ + GQ   +   R   +GV WF      +GL   ++ER+KWEEER 
Sbjct: 301 REVVVDVEVEPEVVKLEGQEIGKNTKRVDENGVFWFAVAEKRIGLRSVVMERMKWEEER- 360

Query: 361 GFEFGKEKEKKYVKVKRREEY-GGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQ 415
              FG + E +   VK+ E + GG   WK + CYVL+E F L+R D SLVLT+EFKH  +
Sbjct: 361 ---FGWKIEPQRAVVKKSERFNGGGSNWKSYRCYVLVESFELRRKDKSLVLTFEFKHVDK 378

BLAST of MS015282 vs. TAIR 10
Match: AT1G13500.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 260.4 bits (664), Expect = 2.5e-69
Identity = 174/429 (40.56%), Postives = 236/429 (55.01%), Query Frame = 0

Query: 1   MYVTRPLSMY-RNEGATSAAPVPAALVEGPNTGVLVIEDEAAES--RWLFGLLKRKSVKA 60
           MYVT+ LS Y RN    +  P      EGPN+GVLVI+DE +    +  F       +  
Sbjct: 1   MYVTKRLSEYKRNPSELTLLP-----TEGPNSGVLVIQDEESRPTLKCCFVNSFDWELNG 60

Query: 61  PPFPQNKIMELRYTINSGEHQHTDYFYALLIPVINRPLSSNKYYVIKSRGKTKGLLISIN 120
            PFPQN  + ++  I +  +     F    IPV+++P SSN YYVI+  GK  G      
Sbjct: 61  LPFPQNTRVIVKRQIGTALYLDPVAF----IPVLHQPPSSNLYYVIRGSGKHTG------ 120

Query: 121 IGFDVIIVQMYEPKRRLACTSSKEDDETSCC-CFG-IPDTPPQLFDPTNAYQQFQISNYV 180
                            AC S+KE D  SCC CF  IP+  P+  DP +  QQF+I  + 
Sbjct: 121 ----------------EACVSAKEGDRASCCFCFTYIPEAVPRPLDPFDINQQFEI--HQ 180

Query: 181 QCAGPCGFMANSIAPDGVPPRFLRRRGWRAYTKTFKNKNLEL-TQALGL-DAALRARLPA 240
             +    F A S+A DG+PP+FL R+GW       + ++  L   A G+ DA LR  LP 
Sbjct: 181 GRSSTKKFFATSVASDGIPPQFLTRKGWSVIFSAPEVEDYGLIDDAKGIVDAKLRYELPD 240

Query: 241 LNFSLPAKSSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGG 300
           L+ S        VVVGKWY PF+FV++G+   Q+  S YY MTLQQ +EE+F C N+G  
Sbjct: 241 LDNS--------VVVGKWYVPFLFVKEGDAKDQMYKSMYYSMTLQQRFEEVFFCENVGNN 300

Query: 301 ER-GVDFDVSVEKEVILIAGQRTDGREVG---DGVVWFGSWG---VGLSLAIVERVKWEE 360
           +R  V  DV VE EV+ + G+R      G   DGVVWF + G   +GL   ++ER+KWEE
Sbjct: 301 KRFEVVVDVEVETEVVKLEGERIARETQGVNSDGVVWFNASGNEKIGLGSVVLERMKWEE 360

Query: 361 ERAGFEFGKEKEKKYVKVKRREEYGGVGE-WKRFGCYVLIERFVLKRMDGSLVLTWEFKH 415
           ER G+   K  E++   +KR E + G G  WK + CYVL+E F LKR DGSLVLT+EFKH
Sbjct: 361 ERFGW-LNKGYEQR-SSIKRTERFEGGGPYWKSYRCYVLVETFELKRTDGSLVLTYEFKH 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149086.13.8e-22994.44uncharacterized protein LOC111017584 [Momordica charantia][more]
XP_022149112.15.8e-15366.35uncharacterized protein LOC111017603 [Momordica charantia][more]
XP_022945636.11.9e-14061.54uncharacterized protein LOC111449821 [Cucurbita moschata][more]
XP_023542039.17.3e-14062.02uncharacterized protein LOC111802013 [Cucurbita pepo subsp. pepo][more]
KAG6574031.11.3e-13961.54hypothetical protein SDJN03_27918, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D7B11.8e-22994.44uncharacterized protein LOC111017584 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1D6Y42.8e-15366.35uncharacterized protein LOC111017603 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1G1G09.4e-14161.54uncharacterized protein LOC111449821 OS=Cucurbita moschata OX=3662 GN=LOC1114498... [more]
A0A6J1HWZ94.3e-13860.82uncharacterized protein LOC111467621 OS=Cucurbita maxima OX=3661 GN=LOC111467621... [more]
A0A6J1G0V43.3e-13057.79uncharacterized protein LOC111449654 OS=Cucurbita moschata OX=3662 GN=LOC1114496... [more]
Match NameE-valueIdentityDescription
AT1G13520.14.5e-7939.58Protein of unknown function (DUF1262) [more]
AT1G13480.16.5e-7840.00Protein of unknown function (DUF1262) [more]
AT1G13540.16.8e-7541.94Protein of unknown function (DUF1262) [more]
AT1G13470.17.0e-7239.67Protein of unknown function (DUF1262) [more]
AT1G13500.12.5e-6940.56Protein of unknown function (DUF1262) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008872Insecticidal crystal toxinPFAMPF05431Toxin_10coord: 246..413
e-value: 3.5E-7
score: 30.1
IPR010683Protein of unknown function DUF1262PFAMPF06880DUF1262coord: 27..112
e-value: 2.0E-23
score: 82.7
NoneNo IPR availablePANTHERPTHR31050:SF14SUBFAMILY NOT NAMEDcoord: 1..414
NoneNo IPR availablePANTHERPTHR31050OS08G0413200 PROTEINcoord: 1..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015282.1MS015282.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0090729 toxin activity