MS013422 (gene) Bitter gourd (TR) v1

BLAST of MS013422 vs. NCBI nr
Match: XP_023000949.1 (uncharacterized protein LOC111495232 [Cucurbita maxima])

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-53
Identity = 113/153 (73.86%), Postives = 130/153 (84.97%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAAF A+YCSLAVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 4   SEIAAFAAIYCSLAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 63

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSLL 120
           WSSS+VALPKAMNTS+P +WW RSH       A  G GDHIRGALLVASLVCG++GVSLL
Sbjct: 64  WSSSDVALPKAMNTSLPSQWWNRSHRDRSEAAADGGSGDHIRGALLVASLVCGVIGVSLL 123

Query: 121 IASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           IASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 124 IASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 153

BLAST of MS013422 vs. NCBI nr
Match: KAG6583852.1 (hypothetical protein SDJN03_19784, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019473.1 hypothetical protein SDJN02_18434, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 218.4 bits (555), Expect = 4.3e-53
Identity = 117/155 (75.48%), Postives = 135/155 (87.10%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAA  AVYCS+AVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 5   SRIAAVAAVYCSVAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 64

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAA--GRGDHIRGALLVASLVCGIVGVS 120
           WSSS+VALPKAMNTS+P +WW RSH   DR+GAAA  G GDHIRGALLVASLVCG++GVS
Sbjct: 65  WSSSDVALPKAMNTSLPSQWWNRSHR--DRSGAAADGGGGDHIRGALLVASLVCGVIGVS 124

Query: 121 LLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           LLIASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 125 LLIASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 154

BLAST of MS013422 vs. NCBI nr
Match: XP_022927447.1 (uncharacterized protein LOC111434271 [Cucurbita moschata])

HSP 1 Score: 218.0 bits (554), Expect = 5.6e-53
Identity = 116/155 (74.84%), Postives = 135/155 (87.10%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAA  A+YCS+AVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 5   SRIAAVAAIYCSVAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 64

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAA--GRGDHIRGALLVASLVCGIVGVS 120
           WSSS+VALPKAMNTS+P +WW RSH   DR+GAAA  G GDHIRGALLVASLVCG++GVS
Sbjct: 65  WSSSDVALPKAMNTSLPSQWWNRSHR--DRSGAAADGGGGDHIRGALLVASLVCGVIGVS 124

Query: 121 LLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           LLIASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 125 LLIASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 154

BLAST of MS013422 vs. NCBI nr
Match: XP_023519911.1 (uncharacterized protein LOC111783236 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 217.6 bits (553), Expect = 7.3e-53
Identity = 116/155 (74.84%), Postives = 134/155 (86.45%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAA  A+YCSLAVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 5   SRIAAVAAIYCSLAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 64

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAA--AGRGDHIRGALLVASLVCGIVGVS 120
           WSSS+VALPKAMNTS+P +WW RSH   DR+GAA   G GDHIRGALLVASLVCG++GVS
Sbjct: 65  WSSSDVALPKAMNTSLPSQWWNRSHR--DRSGAAGDGGGGDHIRGALLVASLVCGVIGVS 124

Query: 121 LLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           LLIASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 125 LLIASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 154

BLAST of MS013422 vs. NCBI nr
Match: XP_038895122.1 (uncharacterized protein LOC120083432 [Benincasa hispida])

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-49
Identity = 109/153 (71.24%), Postives = 126/153 (82.35%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           SQI +F AV+ SLAVI LA G SNAR+LRPSEHGLE+QNP  GA E SSP M+SFF+GNS
Sbjct: 6   SQIPSFAAVFSSLAVILLA-GISNARDLRPSEHGLEYQNP--GAMENSSPQMQSFFKGNS 65

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSLL 120
           WSSS+VALPKAMNTS+PP+WW RSH +     A  G GDHIRGALLVASLVCGI+G+SLL
Sbjct: 66  WSSSDVALPKAMNTSLPPQWWNRSHRNRSGAAADGGGGDHIRGALLVASLVCGIIGISLL 125

Query: 121 IASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           IASAFIY FKF+K   S SSLS+S++TNI+ NK
Sbjct: 126 IASAFIYFFKFRKQSRSSSSLSVSETTNIIPNK 155

BLAST of MS013422 vs. ExPASy TrEMBL
Match: A0A6J1KLE3 (uncharacterized protein LOC111495232 OS=Cucurbita maxima OX=3661 GN=LOC111495232 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 5.5e-54
Identity = 113/153 (73.86%), Postives = 130/153 (84.97%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAAF A+YCSLAVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 4   SEIAAFAAIYCSLAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 63

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSLL 120
           WSSS+VALPKAMNTS+P +WW RSH       A  G GDHIRGALLVASLVCG++GVSLL
Sbjct: 64  WSSSDVALPKAMNTSLPSQWWNRSHRDRSEAAADGGSGDHIRGALLVASLVCGVIGVSLL 123

Query: 121 IASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           IASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 124 IASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 153

BLAST of MS013422 vs. ExPASy TrEMBL
Match: A0A6J1EH69 (uncharacterized protein LOC111434271 OS=Cucurbita moschata OX=3662 GN=LOC111434271 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.7e-53
Identity = 116/155 (74.84%), Postives = 135/155 (87.10%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGNS 60
           S+IAA  A+YCS+AVIFLAGG SNAR+LRPSEHGLE+QNP  GA E SSPDM+SFF+GNS
Sbjct: 5   SRIAAVAAIYCSVAVIFLAGG-SNARDLRPSEHGLEYQNP--GAIENSSPDMQSFFKGNS 64

Query: 61  WSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAA--GRGDHIRGALLVASLVCGIVGVS 120
           WSSS+VALPKAMNTS+P +WW RSH   DR+GAAA  G GDHIRGALLVASLVCG++GVS
Sbjct: 65  WSSSDVALPKAMNTSLPSQWWNRSHR--DRSGAAADGGGGDHIRGALLVASLVCGVIGVS 124

Query: 121 LLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           LLIASAFIY+FKF+K   S SSLS+S++TNI+ NK
Sbjct: 125 LLIASAFIYVFKFRKQNRSSSSLSVSETTNIIANK 154

BLAST of MS013422 vs. ExPASy TrEMBL
Match: A0A6J1CG32 (uncharacterized protein LOC111010910 OS=Momordica charantia OX=3673 GN=LOC111010910 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 9.0e-49
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 0

Query: 52  MKSFFRGNSWSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLV 111
           MKSFFRGNSWSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLV
Sbjct: 1   MKSFFRGNSWSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLV 60

Query: 112 CGIVGVSLLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 154
           CGIVGVSLLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK
Sbjct: 61  CGIVGVSLLIASAFIYIFKFKKHRSSPSSLSLSDSTNIVINK 102

BLAST of MS013422 vs. ExPASy TrEMBL
Match: A0A5D3C2L6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00520 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 7.9e-45
Identity = 103/157 (65.61%), Postives = 125/157 (79.62%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLA-VIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGN 60
           S I +F  +Y SLA +I L    SNAR+LRPSEHGL++QNP   A + SSP M+SFF+GN
Sbjct: 6   SPIPSFAVLYSSLALIILLLAPLSNARDLRPSEHGLDYQNP--DAIQNSSPQMQSFFKGN 65

Query: 61  SWSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSL 120
           SWS+S+VALPKAMNTS+PP+WW RSH   +R+GA+A  GDHIRGALLVASLVCGI+GVSL
Sbjct: 66  SWSNSDVALPKAMNTSLPPQWWNRSHR--NRSGASAAGGDHIRGALLVASLVCGIIGVSL 125

Query: 121 LIASAFIYIFKFKKH---RSSPSSLSLSDSTNIVINK 154
           L+ASAFIY FKF+K     SS SSLS S++TNI+ NK
Sbjct: 126 LVASAFIYFFKFRKQSRSSSSSSSLSASETTNIIANK 158

BLAST of MS013422 vs. ExPASy TrEMBL
Match: A0A1S3BC63 (uncharacterized protein LOC103488324 OS=Cucumis melo OX=3656 GN=LOC103488324 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 7.9e-45
Identity = 103/157 (65.61%), Postives = 125/157 (79.62%), Query Frame = 0

Query: 1   SQIAAFTAVYCSLA-VIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSPDMKSFFRGN 60
           S I +F  +Y SLA +I L    SNAR+LRPSEHGL++QNP   A + SSP M+SFF+GN
Sbjct: 6   SPIPSFAVLYSSLALIILLLAPLSNARDLRPSEHGLDYQNP--DAIQNSSPQMQSFFKGN 65

Query: 61  SWSSSEVALPKAMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSL 120
           SWS+S+VALPKAMNTS+PP+WW RSH   +R+GA+A  GDHIRGALLVASLVCGI+GVSL
Sbjct: 66  SWSNSDVALPKAMNTSLPPQWWNRSHR--NRSGASAAGGDHIRGALLVASLVCGIIGVSL 125

Query: 121 LIASAFIYIFKFKKH---RSSPSSLSLSDSTNIVINK 154
           L+ASAFIY FKF+K     SS SSLS S++TNI+ NK
Sbjct: 126 LVASAFIYFFKFRKQSRSSSSSSSLSASETTNIIANK 158

BLAST of MS013422 vs. TAIR 10
Match: AT4G21740.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G30515.1); Has 20 Blast hits to 20 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 20; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.2 bits (165), Expect = 6.7e-12
Identity = 54/147 (36.73%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 12  SLAVIFLAGGTSNARELRPSEHGLEFQNPGSGAAEKSSP-DMKSFFRGNSWSSS-----E 71
           ++  + +  G S A ELRPS+HGL++Q         S P  MKSFF G+S SSS      
Sbjct: 19  TITFLVIFTGNSLAGELRPSDHGLQYQFSSPPTESHSPPGKMKSFF-GDSHSSSPPPSHP 78

Query: 72  VALPK--AMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRGALLVASLVCGIVGVSLLIAS 131
             LPK  A +      WW        R GA   R   +R   L AS++CG+ GV+LL+  
Sbjct: 79  QLLPKATAADGGDDDSWW--------RDGAGIRRDHVMRHVFLAASIICGVSGVALLVVF 138

Query: 132 AFIYIFKFKKHRSSPSSLSLSDSTNIV 151
             IY F+++KH  S S    +DS  I+
Sbjct: 139 TLIYFFRYRKHNHSNSPTG-NDSKEII 155

BLAST of MS013422 vs. TAIR 10
Match: AT1G30515.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 6 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G21740.1); Has 20 Blast hits to 20 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 20; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.4 bits (124), Expect = 3.8e-07
Identity = 45/128 (35.16%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 15  VIFLAGGTSNARELRPSEHGLE-FQNPGSGAAEKSSPDMKSFF---RGNSWSSSEVALPK 74
           +I L     NARELRPS+HGLE +  PG       S +M SFF     N  +S       
Sbjct: 20  IIVLESTIINARELRPSDHGLEYYYEPG------ESSEMTSFFGPPSSNDLTSISSPSSS 79

Query: 75  AMNTSVPPEWWTRSHHHGDRTGAAAGRGDHIRG-ALLVASLVCGIVGVSLLIASAFIYIF 134
            + ++V     T S    D         D +    L+V SLVCG+ GV+L++ASA IY  
Sbjct: 80  ILPSAVKSPMKTLSKDQDD---------DRVMNHVLVVGSLVCGLSGVALMVASALIYFL 132

Query: 135 KFKKHRSS 138
            + K ++S
Sbjct: 140 GYPKTQNS 132