MS013417 (gene) Bitter gourd (TR) v1

BLAST of MS013417 vs. NCBI nr
Match: XP_022140040.1 (putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 564/568 (99.30%), Postives = 565/568 (99.47%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD--GN 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD  GN
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADY 120
           GNGNSALSSSIKAVCDVTLYPETCNYAFGPL NSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQS 240
           QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 567
           TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

BLAST of MS013417 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 973.4 bits (2515), Expect = 8.4e-280
Identity = 494/567 (87.13%), Postives = 525/567 (92.59%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLV VVVAVVFGTRN S  +D + 
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
           N + ALS+SIKAVCDVTLYP TC  AFGPL NSS LDP QI KLSVQLALGELSR ADY+
Sbjct: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120

Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
           F HAITNSTDN+T LAL++CHELLDLALDHLNISLSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDP L N IANYLKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYEDHQS+
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240

Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
            DWARPILRKLAQRD+RKQADIVVAKD SGKYKTI+AAL+AVP+KSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
           ENVEV KNKWNVV++GDGM AT VT  LN IDGTPTFS+ATFAAKGKGFIAIDMGF+N A
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRC+MDAYQDTLY+HSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
           QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQDSSI PYENLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
           TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKW GV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
           AKKFTVSSFIDGKDWISKAQVPFK+ +
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567

BLAST of MS013417 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 951.4 bits (2458), Expect = 3.4e-273
Identity = 481/566 (84.98%), Postives = 514/566 (90.81%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
            N ALS+SIKAVCD TLYP+TCN AFGPL NSS LDPGQI K SVQLAL ELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
           +HAITNSTDN+T LAL  CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
           DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
           NVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
           NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
           TVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
           KKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of MS013417 vs. NCBI nr
Match: KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 948.0 bits (2449), Expect = 3.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
            N ALS+SIKAVCD TLYP+TC  AFG L NSS LDPGQI K SVQLALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
           +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
           DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
           NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
           NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
           TVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
           KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of MS013417 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 948.0 bits (2449), Expect = 3.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
            N ALS+SIKAVCD TLYP+TC  AFG L NSS LDPGQI K SVQLALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
           +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
           DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
           NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
           NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
           TVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
           KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of MS013417 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.4e-168
Identity = 313/570 (54.91%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKD----DGNGNG 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L  +V+  VFGT    K     + N NG
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  NSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
           +S +S S+KAVCDVTL+ E C    G   N+S L+P ++ + +V++ + E+S+A      
Sbjct: 64  DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123

Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
           +A ++S  D +  + + +C ELLDL +D+LN +L+S+   ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183

Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYEDH 245
           YQ+TC+E L    P +R    ++LKNSTELTSN LAI+++  KI DS  L RRL++  D 
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243

Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
           +      R   R L   DLRK ADIVVAKDGSGKY+TI  AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303

Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
            Y ENV+V K  WNV+++GDG + + V+ +LN IDGTPTF +ATFA  GKGF+A DMGF 
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363

Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
           N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423

Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
            VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI   +I+P  +L    T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483

Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
           N+STTV M +YL   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG+R + 
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543

Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
            K A +FTV  FIDG  W+   +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of MS013417 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 8.5e-166
Identity = 304/566 (53.71%), Postives = 402/566 (71.02%), Query Frame = 0

Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNSA 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT  R+ SK     N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
           +S S+KA+CDVTL+ E C    G   N+SR  P ++ K +V++ + ELS+  D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
               DN T  A+ +C EL+ LA+D LN +++S+    LK  DDL+TWL+S  TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSADWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  KI D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
             P++   R L   DL+K+A IVVAKDGSGKY+TI  AL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
           ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA  GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
           Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
           TTV M++++   I P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G++ ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 565
            A KFTV  FIDG +W+   +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of MS013417 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-136
Identity = 268/582 (46.05%), Postives = 373/582 (64.09%), Query Frame = 0

Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFG-------TRNGSKDDGNG 66
           YGKV+E +Q   +  ++TRKR+ +I +SS+VL+AV++A V G       + +    D   
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 126
               + ++S+KAVCD T YP +C  +   L  S+  DP  + KLS+++A+ ELS    + 
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELS---SFP 129

Query: 127 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSST-----EITLLKAVDDLKTWLTS 186
            K       D R + A++ C  +   ALD LN S+S+       I    +V +++TWL++
Sbjct: 130 SKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIEGLEEVDPTLR----NSIANYLKNSTELTSNGLAIVS----FFSKITDSLN 246
           A T Q TC++ + E++ T        I   ++NSTE  SN LAIV+      S+    ++
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 LRRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSK 306
            RRL+ + +   +A+      R L +++     D VVAKDGSG++KTI  ALK V  KS+
Sbjct: 250 HRRLLGFPEWLGAAE-----RRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSE 309

Query: 307 KSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGK 366
           +   +YVK+G Y EN+++ KN WNV++ GDG + T V    NF+DGTPTF +ATFA KGK
Sbjct: 310 ERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGK 369

Query: 367 GFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEG 426
           GFIA D+GF N AG SKHQAVAL S SD+S+F+RC  D +QDTLYAHSNRQFYR+C + G
Sbjct: 370 GFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITG 429

Query: 427 TVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKA 486
           T+DFIFGN+AVV Q+C I+P+ PL  Q NTITAQG+ DPNQNTGI IQ S+ITP+ N   
Sbjct: 430 TIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLT 489

Query: 487 TETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSSTAK 546
             TYLGRPWK++STTV MQ+ +G+L+ P GW+ WV     P TIFYAE+QN GPG+  ++
Sbjct: 490 APTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQ 549

Query: 547 RVKWKGVR-NIDNKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
           RVKW G +  I ++ A++FTV SFI G +W+  A V F S L
Sbjct: 550 RVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of MS013417 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 8.6e-126
Identity = 254/573 (44.33%), Postives = 362/573 (63.18%), Query Frame = 0

Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT---RNGSKDDGNGNGNS 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+AV++A V  T   +N ++   +     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
             S+S+KA+C VT +PE+C  +   L +S+  DP  + KLS+++ + EL   +D   K +
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129

Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
              + D R K AL  C +L++ ALD LN ++S+ +    K       ++DLKTWL++  T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
             +TC + L+E+        + T+  ++ + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
              RRLMS+  HQ S D+ +   R+L Q    K  D+ VA DG+G   T++ A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
           S K  VIYVK GTY ENV + K+KWNV++ GDG   T ++   NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
           GKGFI  D+G  N AG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I+   N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
            A  TYLGRPWK +STTV M+  +G++++P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 556
            +RVKW G + +  +  A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of MS013417 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 402.5 bits (1033), Expect = 7.8e-111
Identity = 235/564 (41.67%), Postives = 343/564 (60.82%), Query Frame = 0

Query: 19  DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNSALSSSIKAVCDV--- 78
           D S+R R+ I VI +SSV+L+++VVAV  G  + +K DG+  G + +++S+KAV DV   
Sbjct: 7   DESKRKRRYI-VITISSVLLISMVVAVTVGV-SLNKHDGDSKGKAEVNASVKAVKDVCAP 66

Query: 79  TLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTKLA 138
           T Y +TC          +  DP ++ K +  + + +++ AA    +  +    D+RT++A
Sbjct: 67  TDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTRMA 126

Query: 139 LESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEVDP 198
           L+ C EL+D ALD L+ S   L   E  LL +A+ +L+ WL++A ++++TC+EG +    
Sbjct: 127 LDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQG 186

Query: 199 TLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYEDHQSSADWARPI 258
               ++   LK + ELT NGLAI+S  S          LN RRL++ E   S  D     
Sbjct: 187 NAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRGRK 246

Query: 259 LRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEVGK 318
           L + A      + DIVVA+DGSG+YKTI+ AL+ VP K   + V+++K G YKE V+V K
Sbjct: 247 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 306

Query: 319 NKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKHQA 378
              ++V IGDG + T ++   N+ DG  T+ +AT A  G  FIA ++GF+N AG  KHQA
Sbjct: 307 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 366

Query: 379 VALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTILP 438
           VA+   SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+L 
Sbjct: 367 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 426

Query: 439 KLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYSTTV 498
           + PL  Q   ITA GR DP ++TG   Q  +I     Y  +K T + YLGRPWK YS T+
Sbjct: 427 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 486

Query: 499 FMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTAKK 558
            M  ++   +QP GW PW+G     T+FY+E QN GPGS+ A RV W G++ +  +   K
Sbjct: 487 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 546

Query: 559 FTVSSFIDGKDWISKAQVPFKSGL 567
           FT + +I G DWI    VP+ +GL
Sbjct: 547 FTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of MS013417 vs. ExPASy TrEMBL
Match: A0A6J1CDY3 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 564/568 (99.30%), Postives = 565/568 (99.47%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD--GN 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD  GN
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADY 120
           GNGNSALSSSIKAVCDVTLYPETCNYAFGPL NSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQS 240
           QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 567
           TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

BLAST of MS013417 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 481/566 (84.98%), Postives = 514/566 (90.81%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
            N ALS+SIKAVCD TLYP+TCN AFGPL NSS LDPGQI K SVQLAL ELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
           +HAITNSTDN+T LAL  CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
           DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
           NVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
           NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
           TVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
           KKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of MS013417 vs. ExPASy TrEMBL
Match: A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
            N ALS+SIKAVCD TLYP+TC  AFG L NSS LDPGQI K SVQLALGELSR ADY+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
           +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
           TCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED  S+ 
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
           DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
           NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
           NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
           TVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
           KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of MS013417 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 477/567 (84.13%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S  +D + 
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
           N N +LS+SIKAVCDVTLYP+TC  AFGP  NSS LDPGQI KL+VQLALGELS+ AD++
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
           F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDP L +  AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
            DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
           E VEV KNKWNVVM+GDGMNAT V+  LN IDGTPTFS+A+FAAKGKGFIAIDMGF N A
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
           QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
           TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
           AKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of MS013417 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 477/567 (84.13%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S  +D + 
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
           N N +LS+SIKAVCDVTLYP+TC  AFGP  NSS LDPGQI KL+VQLALGELS+ AD++
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
           F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
           QTCI+GL EVDP L +  AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
            DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
           E VEV KNKWNVVM+GDGMNAT V+  LN IDGTPTFS+A+FAAKGKGFIAIDMGF N A
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
           QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
           TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
           AKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of MS013417 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 593.6 bits (1529), Expect = 1.7e-169
Identity = 313/570 (54.91%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKD----DGNGNG 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L  +V+  VFGT    K     + N NG
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  NSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
           +S +S S+KAVCDVTL+ E C    G   N+S L+P ++ + +V++ + E+S+A      
Sbjct: 64  DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123

Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
           +A ++S  D +  + + +C ELLDL +D+LN +L+S+   ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183

Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYEDH 245
           YQ+TC+E L    P +R    ++LKNSTELTSN LAI+++  KI DS  L RRL++  D 
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243

Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
           +      R   R L   DLRK ADIVVAKDGSGKY+TI  AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303

Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
            Y ENV+V K  WNV+++GDG + + V+ +LN IDGTPTF +ATFA  GKGF+A DMGF 
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363

Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
           N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423

Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
            VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI   +I+P  +L    T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483

Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
           N+STTV M +YL   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG+R + 
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543

Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
            K A +FTV  FIDG  W+   +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of MS013417 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 585.1 bits (1507), Expect = 6.0e-167
Identity = 304/566 (53.71%), Postives = 402/566 (71.02%), Query Frame = 0

Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNSA 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT  R+ SK     N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
           +S S+KA+CDVTL+ E C    G   N+SR  P ++ K +V++ + ELS+  D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
               DN T  A+ +C EL+ LA+D LN +++S+    LK  DDL+TWL+S  TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSADWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  KI D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
             P++   R L   DL+K+A IVVAKDGSGKY+TI  AL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
           ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA  GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
           Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
           TTV M++++   I P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G++ ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 565
            A KFTV  FIDG +W+   +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of MS013417 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 452.2 bits (1162), Expect = 6.1e-127
Identity = 254/573 (44.33%), Postives = 362/573 (63.18%), Query Frame = 0

Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT---RNGSKDDGNGNGNS 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+AV++A V  T   +N ++   +     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
             S+S+KA+C VT +PE+C  +   L +S+  DP  + KLS+++ + EL   +D   K +
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129

Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
              + D R K AL  C +L++ ALD LN ++S+ +    K       ++DLKTWL++  T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
             +TC + L+E+        + T+  ++ + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
              RRLMS+  HQ S D+ +   R+L Q    K  D+ VA DG+G   T++ A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
           S K  VIYVK GTY ENV + K+KWNV++ GDG   T ++   NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
           GKGFI  D+G  N AG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I+   N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
            A  TYLGRPWK +STTV M+  +G++++P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 556
            +RVKW G + +  +  A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of MS013417 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 402.5 bits (1033), Expect = 5.5e-112
Identity = 235/564 (41.67%), Postives = 343/564 (60.82%), Query Frame = 0

Query: 19  DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNSALSSSIKAVCDV--- 78
           D S+R R+ I VI +SSV+L+++VVAV  G  + +K DG+  G + +++S+KAV DV   
Sbjct: 7   DESKRKRRYI-VITISSVLLISMVVAVTVGV-SLNKHDGDSKGKAEVNASVKAVKDVCAP 66

Query: 79  TLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTKLA 138
           T Y +TC          +  DP ++ K +  + + +++ AA    +  +    D+RT++A
Sbjct: 67  TDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTRMA 126

Query: 139 LESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEVDP 198
           L+ C EL+D ALD L+ S   L   E  LL +A+ +L+ WL++A ++++TC+EG +    
Sbjct: 127 LDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQG 186

Query: 199 TLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYEDHQSSADWARPI 258
               ++   LK + ELT NGLAI+S  S          LN RRL++ E   S  D     
Sbjct: 187 NAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRGRK 246

Query: 259 LRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEVGK 318
           L + A      + DIVVA+DGSG+YKTI+ AL+ VP K   + V+++K G YKE V+V K
Sbjct: 247 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 306

Query: 319 NKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKHQA 378
              ++V IGDG + T ++   N+ DG  T+ +AT A  G  FIA ++GF+N AG  KHQA
Sbjct: 307 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 366

Query: 379 VALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTILP 438
           VA+   SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+L 
Sbjct: 367 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 426

Query: 439 KLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYSTTV 498
           + PL  Q   ITA GR DP ++TG   Q  +I     Y  +K T + YLGRPWK YS T+
Sbjct: 427 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 486

Query: 499 FMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTAKK 558
            M  ++   +QP GW PW+G     T+FY+E QN GPGS+ A RV W G++ +  +   K
Sbjct: 487 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 546

Query: 559 FTVSSFIDGKDWISKAQVPFKSGL 567
           FT + +I G DWI    VP+ +GL
Sbjct: 547 FTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of MS013417 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.5e-109
Identity = 231/512 (45.12%), Postives = 308/512 (60.16%), Query Frame = 0

Query: 66  SSSIKAVCDVTLYPETCNYAFGPL-YNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 125
           SS ++ VC+VT YP +C  +   L  + +  DP  + +LS+Q+   EL+       K A 
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVGLPKKLA- 515

Query: 126 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITL--------LKAVDDLKTWLTSAA 185
             + D   K AL  C ++ DLA+D +N ++SS +  +           + DL TWL+SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 186 TYQQTCIEGLEE--VDPTLRNSIANYLKNSTELTSNGLAIVSFF----SKITDSLNLRRL 245
           T   TC + L+E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 246 MSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTV 305
           +   +  S  +W RP +R+L Q        + VA DGSG  +T++ A+  VP K K   V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695

Query: 306 IYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIA 365
           IYVK GTY ENV + K+KWNV + GDG + T ++   N +DG  TF+++TFA +GKGF+ 
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755

Query: 366 IDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDF 425
            DMG  N AGP KHQAVA  S SD+S++YRC  D YQDTLY HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815

Query: 426 IFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETY 485
           IFG   VV Q C+I P+ PL  Q NTITA+G  + NQNTGISI   +I+P  N+ AT TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNVTAT-TY 875

Query: 486 LGRPWKNYSTTVFMQNYLGSLIQPTGWLPWVGT--SAPDTIFYAEFQNYGPGSSTAKRVK 545
           LGRPWK +S TV MQ+ +GS + P GW+ W  T    P TIFY E++N GPGS  +KRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935

Query: 546 WKGVRNI-DNKTAKKFTVSSFIDGKD-WISKA 559
           W G + I  +  A +FTV  F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961