MS013393 (gene) Bitter gourd (TR) v1

BLAST of MS013393 vs. NCBI nr
Match: XP_022142602.1 (uncharacterized protein LOC111012678 [Momordica charantia])

HSP 1 Score: 578.6 bits (1490), Expect = 3.1e-161
Identity = 289/291 (99.31%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN
Sbjct: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNL+I
Sbjct: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLVI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIPGVLQPETTLTKVEKTSLLHAYEVN+VGPILVIKHLWPFLKAGGGSGTERDV
Sbjct: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNSVGPILVIKHLWPFLKAGGGSGTERDV 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 291

BLAST of MS013393 vs. NCBI nr
Match: KAG6573431.1 (hypothetical protein SDJN03_27318, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012579.1 hypothetical protein SDJN02_25331 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538.5 bits (1386), Expect = 3.6e-149
Identity = 272/291 (93.47%), Postives = 281/291 (96.56%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKL + LNQPMFAAGTLRSL ARRA+FSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KN
Sbjct: 1   MKLQL-LNQPMFAAGTLRSL-ARRASFSSLAAKWEGGVSMVQGASRGIGLEFVRHLLEKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPG++TGL+ELKDKFSERLCILELDVT ESTIK SAKSI ERYGSLNLLI
Sbjct: 61  EKGHVVATCRNPGKATGLLELKDKFSERLCILELDVTNESTIKESAKSIGERYGSLNLLI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIP VLQPETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD+
Sbjct: 121 NASGILSIPNVLQPETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDI 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. NCBI nr
Match: XP_023541069.1 (uncharacterized protein LOC111801341 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 271/291 (93.13%), Postives = 280/291 (96.22%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKL + LN PMFAAGTLRSL ARRA+FSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KN
Sbjct: 1   MKLQL-LNHPMFAAGTLRSL-ARRASFSSLAAKWEGGVSMVQGASRGIGLEFVRHLLEKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPG++TGL+ELKDKFSERLCILELDVT ESTIK SAKSI ERYGSLNLLI
Sbjct: 61  EKGHVVATCRNPGKATGLLELKDKFSERLCILELDVTNESTIKESAKSIGERYGSLNLLI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIP VLQPETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD+
Sbjct: 121 NASGILSIPNVLQPETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDI 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. NCBI nr
Match: XP_022955063.1 (uncharacterized protein LOC111457138 [Cucurbita moschata])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 271/291 (93.13%), Postives = 281/291 (96.56%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKL + LNQPMFAAGTLRSL ARRA+FSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KN
Sbjct: 1   MKLQL-LNQPMFAAGTLRSL-ARRASFSSLAAKWEGGVSMVQGASRGIGLEFVRHLLEKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPG++TGL+ELKDKFSERLCILELDVT ESTIK SAKSI ERYGSLNLLI
Sbjct: 61  EKGHVVATCRNPGKATGLLELKDKFSERLCILELDVTNESTIKESAKSIGERYGSLNLLI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIP VLQPETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD+
Sbjct: 121 NASGILSIPNVLQPETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDI 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTK+FSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKNFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. NCBI nr
Match: XP_038894686.1 (C-factor [Benincasa hispida])

HSP 1 Score: 531.6 bits (1368), Expect = 4.4e-147
Identity = 266/291 (91.41%), Postives = 280/291 (96.22%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           M LL+  NQPMFAAG L+SL ARR NFSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KN
Sbjct: 1   MNLLLH-NQPMFAAGILKSL-ARRPNFSSVAAKWEGGVSMVQGASRGIGLEFVRHLLEKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPG++TGL+ELK+KF+ERLCILELDVT E+TIKASAKSIEERYGSLNLLI
Sbjct: 61  EKGHVVATCRNPGQATGLLELKNKFAERLCILELDVTNETTIKASAKSIEERYGSLNLLI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIP VLQPETTL KVE++SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV
Sbjct: 121 NASGILSIPNVLQPETTLNKVERSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTK++SVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKEYSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. ExPASy Swiss-Prot
Match: Q7Z9I2 (Uncharacterized oxidoreductase C663.09c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC663.09c PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 9.2e-23
Identity = 81/264 (30.68%), Postives = 127/264 (48.11%), Query Frame = 0

Query: 38  VSMVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIELKDKFSERLCILELDVT 97
           V  + G +RGIGL  V+  L   E   V A+ R P  +T L E   K    +  +ELDVT
Sbjct: 7   VYFIAGGNRGIGLSLVKE-LSSREGTTVFASARKPEAATELQEW-SKSHPNVKTVELDVT 66

Query: 98  KESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGP 157
            + +   +A+S+ +    +++L   SGI       Q   T+ +  +      Y+ N +GP
Sbjct: 67  SQQSANEAAQSVAKAVDGIDVLWLNSGI------CQSYYTVMEAPEEVWNAHYQTNVLGP 126

Query: 158 ILVIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKN 217
           I V K  +P L       T++    V   S+  GS+GD    G+ +Y  SK A+N   K 
Sbjct: 127 IHVFKAFYPLL-------TKKKTRQVIFTSSECGSMGDFRATGFSAYGQSKAAINFTMKE 186

Query: 218 VSVEFARKKDPIICILLHPGTVDTDLS----RPFQRNVPEGKLFTK------DFSVQKLM 277
           +SVE A +    I I  HPG V TD++    + F    PE   + K      + SV  ++
Sbjct: 187 LSVELADEHFTFISI--HPGVVKTDMNADAIKKFTETSPEMLTYLKKVTIIPEESVSSML 246

Query: 278 TIINNAKSQDNGKFFAWDGQEIPW 292
            +++N K ++NG F+ +DG  IP+
Sbjct: 247 KVVDNLKPENNGSFYRYDGTIIPF 253

BLAST of MS013393 vs. ExPASy Swiss-Prot
Match: Q9P7I6 (Uncharacterized oxidoreductase C24B10.20 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC24B10.20 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.0e-21
Identity = 79/268 (29.48%), Postives = 133/268 (49.63%), Query Frame = 0

Query: 36  GGVSMVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIELKD--KFSERLCILE 95
           G V ++ G +RGIGL  V+ L  K E   V A+ R PG ++   ELKD  K    + I++
Sbjct: 6   GIVYVIVGGNRGIGLSLVKELSNK-EGVTVFASARGPGSAS---ELKDWSKTHSNVHIIK 65

Query: 96  LDVTKESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVN 155
           LDVT   + K +A  +E+    +++L   SGI       QP   + K      +  Y+ N
Sbjct: 66  LDVTSLRSAKDAAMQVEKVVKCIDVLWVNSGISK---SFQP---VLKTSDELWMSHYQTN 125

Query: 156 AVGPILVIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQ 215
            +GPI V +  +  LK     G  +++   ++++A +G +  N    + +Y  SK ALN 
Sbjct: 126 VLGPIHVYQAFYHLLK----EGKLKNIVFTSSMAACMGGVRPNT---YSAYGQSKAALNY 185

Query: 216 LTKNVSVEFARKKDPIICILLHPGTVDTDL----SRPFQRNVPE------GKLFTKDFSV 275
             K +S E   +KD  + + +HPG V+TD+     +      PE          + + S 
Sbjct: 186 TMKEISFEL--EKDGFVVVSIHPGVVNTDMFVNAMQKLASKYPEMVESIKSNAISPEQSA 245

Query: 276 QKLMTIINNAKSQDNGKFFAWDGQEIPW 292
             ++ I++N K++DNG F+  DG ++P+
Sbjct: 246 SSMLKIVDNLKTEDNGMFYNLDGTKLPF 254

BLAST of MS013393 vs. ExPASy Swiss-Prot
Match: Q7Z9I4 (Uncharacterized oxidoreductase C663.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC663.06c PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.8e-19
Identity = 78/265 (29.43%), Postives = 125/265 (47.17%), Query Frame = 0

Query: 38  VSMVQGASRGIGLQFVRHLLQKNEKGHVV-ATCRNPGESTGLIELKDKFSERLCILELDV 97
           +  + G +RGIGL  V+ L   N +G VV A+ R P  +T L E   K    + I++LD+
Sbjct: 7   IYFIAGGNRGIGLSLVKEL--SNREGTVVFASARKPEAATELQEW-SKSHSNVHIIKLDI 66

Query: 98  TKESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVG 157
           +   +   +A+ + +  G +++L   SGI          T L   +     H Y+ N +G
Sbjct: 67  SSLESANEAAQEVAKAVGKVDVLWVNSGIFH-----SFNTVLNTPDDVWNSH-YKTNVLG 126

Query: 158 PILVIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTK 217
           PI V +  +P +K G       +  ++   S+ VGS+G         Y  SK ALN   K
Sbjct: 127 PIHVYQAFYPLVKKG-------ESKIIVFTSSLVGSMGAFFPFNQSGYGQSKAALNFTMK 186

Query: 218 NVSVEFARKKDPIICILLHPGTVDTDLSRPFQRNVPEGK-----LFTK-----DFSVQKL 277
            +S E   + +  I I +HPG V TD ++       E K     +F K     D S   +
Sbjct: 187 EISFEL--QDEGFIVISIHPGMVRTDSAQEAVNQHAEAKPEILDIFAKQALAPDQSASDM 246

Query: 278 MTIINNAKSQDNGKFFAWDGQEIPW 292
           + +++N K ++NG FF +DG  IP+
Sbjct: 247 LKVVDNLKPENNGFFFNYDGTTIPY 253

BLAST of MS013393 vs. ExPASy Swiss-Prot
Match: Q9RBP5 (1-hydroxy-2-glutathionyl-2-methyl-3-butene dehydrogenase OS=Rhodococcus sp. (strain AD45) OX=103808 GN=isoH PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 6.1e-19
Identity = 71/252 (28.17%), Postives = 118/252 (46.83%), Query Frame = 0

Query: 40  MVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKE 99
           +V GA +GI     R L  + E   V+A C   G       +  +         +DVT +
Sbjct: 5   LVVGADKGIAHSISRQLHDRGE--DVIAACLFDGADLAAAGITVEPG-------VDVTSQ 64

Query: 100 STIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPIL 159
            +++A A  + E+   L+ + + +G++ +  V   +  L +          E+N +GP+ 
Sbjct: 65  ESVEALAARLSEKGVKLDAVFHVAGVMWLDEVGSLDYDLIR-------RQIEINTLGPLR 124

Query: 160 VIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVS 219
            I+ + P L  G         A V  +++RVGS+GDN  GG +SYR SK A N +  N  
Sbjct: 125 TIEAVRPLLNEG---------AKVGIVTSRVGSLGDNTSGGMYSYRISKAAANMVGLNFH 184

Query: 220 VEFARKKDPIICILLHPGTVDTDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNG 279
            + +  KD +  +LLHPG V TDL++ F     E    T + +   L+  I+N   + +G
Sbjct: 185 HDLS--KDGVSVLLLHPGMVATDLTKDFP---GEHSYITPEQAAAGLIKNIDNLTPETSG 226

Query: 280 KFFAWDGQEIPW 292
           +F   DG  + W
Sbjct: 245 RFQHSDGTFLQW 226

BLAST of MS013393 vs. ExPASy Swiss-Prot
Match: Q7Z9I3 (Uncharacterized oxidoreductase C663.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC663.08c PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.4e-18
Identity = 77/267 (28.84%), Postives = 127/267 (47.57%), Query Frame = 0

Query: 38  VSMVQGASRGIGLQFVRHLLQKNEKGHVV-ATCRNPGESTGLIELKDKFSERLCILELDV 97
           +  + G +RGIGL  V+ L   N KG VV A+ R PG +T L E   K    + I++LDV
Sbjct: 7   IYFIAGGNRGIGLSLVKEL--SNRKGTVVFASARKPGAATKLQEW-SKSHSNVHIIKLDV 66

Query: 98  TKESTIKASAKSIEERYGSLNLL-INASGILSI-PGVLQPETTLTKVEKTSLLHAYEVNA 157
           +   +   +A+ + +   ++++L +N++   S  P V  P+              Y+ N 
Sbjct: 67  SSLESANEAAQEVTKVVDAVDVLWVNSAVFHSFGPVVNTPDDVWNS--------HYKTNV 126

Query: 158 VGPILVIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQL 217
           +GPI V +  +P +K G          ++   S+  GS+G        +Y  SK ALN  
Sbjct: 127 LGPIHVYQAFYPLIKKGRSK-------IIVFTSSLAGSMGAFFPSSQSAYGQSKAALNYT 186

Query: 218 TKNVSVEFARKKDPIICILLHPGTVDTDLSRPFQRNVPEGK-----LFTK-----DFSVQ 277
            K +S E   + +  I I +HPG V TD ++       E K     LF K     + S  
Sbjct: 187 MKEISFEL--QDEGFIVISIHPGAVRTDSAQEIVNQHAEKKPEILDLFAKQALTPEKSAS 246

Query: 278 KLMTIINNAKSQDNGKFFAWDGQEIPW 292
            ++ +++N K ++NG F+ +DG  IP+
Sbjct: 247 DMLKVVDNLKPENNGLFYNYDGTIIPF 253

BLAST of MS013393 vs. ExPASy TrEMBL
Match: A0A6J1CNB5 (uncharacterized protein LOC111012678 OS=Momordica charantia OX=3673 GN=LOC111012678 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.5e-161
Identity = 289/291 (99.31%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN
Sbjct: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNL+I
Sbjct: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLVI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIPGVLQPETTLTKVEKTSLLHAYEVN+VGPILVIKHLWPFLKAGGGSGTERDV
Sbjct: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNSVGPILVIKHLWPFLKAGGGSGTERDV 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 291

BLAST of MS013393 vs. ExPASy TrEMBL
Match: A0A6J1GSX0 (uncharacterized protein LOC111457138 OS=Cucurbita moschata OX=3662 GN=LOC111457138 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.6e-149
Identity = 271/291 (93.13%), Postives = 281/291 (96.56%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKN 60
           MKL + LNQPMFAAGTLRSL ARRA+FSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KN
Sbjct: 1   MKLQL-LNQPMFAAGTLRSL-ARRASFSSLAAKWEGGVSMVQGASRGIGLEFVRHLLEKN 60

Query: 61  EKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLI 120
           EKGHVVATCRNPG++TGL+ELKDKFSERLCILELDVT ESTIK SAKSI ERYGSLNLLI
Sbjct: 61  EKGHVVATCRNPGKATGLLELKDKFSERLCILELDVTNESTIKESAKSIGERYGSLNLLI 120

Query: 121 NASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDV 180
           NASGILSIP VLQPETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD+
Sbjct: 121 NASGILSIPNVLQPETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDI 180

Query: 181 AVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240
           AVVANLSARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD
Sbjct: 181 AVVANLSARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVD 240

Query: 241 TDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           TDLSRPFQRNVPEGKLFTK+FSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 241 TDLSRPFQRNVPEGKLFTKNFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. ExPASy TrEMBL
Match: A0A6J1K0G2 (uncharacterized protein LOC111490457 OS=Cucurbita maxima OX=3661 GN=LOC111490457 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 8.9e-146
Identity = 262/285 (91.93%), Postives = 274/285 (96.14%), Query Frame = 0

Query: 7   LNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKNEKGHVV 66
           LNQPMFAAGTLRSL ARRA+FSS AAKWEGGVSMVQGASRGIGL+FVRHLL+KNEKGHVV
Sbjct: 6   LNQPMFAAGTLRSL-ARRASFSSLAAKWEGGVSMVQGASRGIGLEFVRHLLEKNEKGHVV 65

Query: 67  ATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLINASGIL 126
           ATCR+PG++TGL+ELKDKFSERLCILELDVT ES+IK SAKSI ERYGSLNLLINASGIL
Sbjct: 66  ATCRSPGKATGLLELKDKFSERLCILELDVTIESSIKESAKSIGERYGSLNLLINASGIL 125

Query: 127 SIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDVAVVANL 186
           SIP VLQ ETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD+AVVANL
Sbjct: 126 SIPNVLQTETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDIAVVANL 185

Query: 187 SARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDLSRP 246
           SARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPI+CILLHPGTVDTDLSRP
Sbjct: 186 SARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIVCILLHPGTVDTDLSRP 245

Query: 247 FQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           FQRN+PEGKLFTKDFSVQKLMTIINN K QDNGKFFAWDGQEIPW
Sbjct: 246 FQRNIPEGKLFTKDFSVQKLMTIINNTKIQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. ExPASy TrEMBL
Match: A0A1S3BEG0 (C-factor OS=Cucumis melo OX=3656 GN=LOC103488731 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.5e-142
Identity = 256/288 (88.89%), Postives = 270/288 (93.75%), Query Frame = 0

Query: 4   LIRLNQPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKNEKG 63
           L+ LN PMFAA TLRS  AR   FSS +  WEGGVSMVQGASRGIGL+FV+HLL+ NEKG
Sbjct: 3   LLPLNHPMFAARTLRSF-ARSPTFSSLSPNWEGGVSMVQGASRGIGLEFVKHLLENNEKG 62

Query: 64  HVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLINAS 123
           HVVATCRNPG++TGL+ELK+KF ERLCILELDVT E+TIKASAKSIEERYGSLNLLINAS
Sbjct: 63  HVVATCRNPGQATGLLELKNKFDERLCILELDVTNETTIKASAKSIEERYGSLNLLINAS 122

Query: 124 GILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDVAVV 183
           GILSIP VL PETTL KVEK+SLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTER+VAVV
Sbjct: 123 GILSIPNVLLPETTLNKVEKSSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTEREVAVV 182

Query: 184 ANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDL 243
           AN+SARVGSIGDN LGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDL
Sbjct: 183 ANISARVGSIGDNRLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDL 242

Query: 244 SRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           SRPFQRNVPEGKLFTK+FSVQKLMTIINNAKSQDNGKFFAWDGQEIPW
Sbjct: 243 SRPFQRNVPEGKLFTKEFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. ExPASy TrEMBL
Match: D7TKS1 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_10s0003g05770 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.0e-125
Identity = 231/292 (79.11%), Postives = 260/292 (89.04%), Query Frame = 0

Query: 1   MKLLIRLNQPMFAAGTLRSLAARRANFSS-AAAKWEGGVSMVQGASRGIGLQFVRHLLQK 60
           MKLL+ LN   F A  L S+  RRA  S+ +A KWEGGVSMVQGASRGIGL+FV+ LL+K
Sbjct: 1   MKLLL-LNHRSFMAAPLGSV--RRALLSTCSAVKWEGGVSMVQGASRGIGLEFVKQLLEK 60

Query: 61  NEKGHVVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLL 120
           NEKGHV+ATCRNP  +T L++LK++F+ERL IL+LD+T ESTI+ASA SI ERYGSLNLL
Sbjct: 61  NEKGHVIATCRNPDGATALLDLKNEFAERLNILQLDLTIESTIEASANSIRERYGSLNLL 120

Query: 121 INASGILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERD 180
           INASGILSIP +LQPETTL+KV+K+SLL AYEVNAVGPILVIKH+WP L AGGGSGTERD
Sbjct: 121 INASGILSIPNILQPETTLSKVQKSSLLLAYEVNAVGPILVIKHMWPLLTAGGGSGTERD 180

Query: 181 VAVVANLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTV 240
           VAVVANLSARVGSIGDN LGGWHSYR+SK ALNQLTK +SVEF RKKDP+IC+LLHPGTV
Sbjct: 181 VAVVANLSARVGSIGDNRLGGWHSYRSSKAALNQLTKTISVEFVRKKDPVICLLLHPGTV 240

Query: 241 DTDLSRPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           DTDLSRPFQRNVPEGKLFTK+FSV KL++IINNAKS DNGKFFAWDGQEIPW
Sbjct: 241 DTDLSRPFQRNVPEGKLFTKEFSVNKLLSIINNAKSHDNGKFFAWDGQEIPW 289

BLAST of MS013393 vs. TAIR 10
Match: AT4G20760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 8.8e-122
Identity = 212/287 (73.87%), Postives = 248/287 (86.41%), Query Frame = 0

Query: 7   LNQPMFAAGTLRSL--AARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKNEKGH 66
           L  P  A   L+ +  +    + SS+   W+GGVSMVQGASRGIGL+FVR LL+ N+ G+
Sbjct: 12  LVSPSMAVAALKRMRVSGLATSSSSSVPNWKGGVSMVQGASRGIGLEFVRQLLENNKNGY 71

Query: 67  VVATCRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLINASG 126
           VVATCRNP E+T L +LK++FSERL I +LDVT E+TI+ SA+S+ E+YGSLNLLINA+G
Sbjct: 72  VVATCRNPKEATSLSDLKNRFSERLFIQKLDVTDETTIEESAESVREKYGSLNLLINAAG 131

Query: 127 ILSIPGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDVAVVA 186
           ILSIPGVLQPETTL KVEK+SL+ AYEVNAVGPILV+KH+WP LKAGGGSGT+R+VAVVA
Sbjct: 132 ILSIPGVLQPETTLNKVEKSSLMLAYEVNAVGPILVMKHMWPLLKAGGGSGTDREVAVVA 191

Query: 187 NLSARVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDLS 246
           NLSARVGSIGDN LGGWHSYRASK+ALNQLTKNVSVE  R+KDP+ CILLHPGTVDTDLS
Sbjct: 192 NLSARVGSIGDNRLGGWHSYRASKSALNQLTKNVSVELGRRKDPVACILLHPGTVDTDLS 251

Query: 247 RPFQRNVPEGKLFTKDFSVQKLMTIINNAKSQDNGKFFAWDGQEIPW 292
           RPFQRNVP+GKLFT+++SVQKL+ IINN K QD+GKFFAWDGQEIPW
Sbjct: 252 RPFQRNVPDGKLFTREYSVQKLLHIINNTKKQDSGKFFAWDGQEIPW 298

BLAST of MS013393 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 4.1e-10
Identity = 50/183 (27.32%), Postives = 84/183 (45.90%), Query Frame = 0

Query: 35  EGGVSMVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIELKDKFSERLCILEL 94
           +G ++++ G + GIG + VR       K  +V      G++  +   KDK S   C    
Sbjct: 7   DGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRC---- 66

Query: 95  DVTKESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVNA 154
           DVT E  ++ + K   E+YG L++L + +G++  PG    +  L + ++T       VN 
Sbjct: 67  DVTNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPGSFL-DLNLEQFDRT-----MAVNV 126

Query: 155 VGPILVIKHLWPFLKAGGGSGTERDVAVVANLSARVGSIGDNCLGGWHSYRASKTALNQL 214
            G    IKH     +A    GT   +    ++++ +G  G       H+Y ASK AL  L
Sbjct: 127 RGAAAFIKHA---ARAMVEKGTRGSIVCTTSVASEIGGPGP------HAYTASKHALLGL 170

Query: 215 TKN 218
            K+
Sbjct: 187 VKS 170

BLAST of MS013393 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 3.5e-09
Identity = 60/243 (24.69%), Postives = 101/243 (41.56%), Query Frame = 0

Query: 9   QPMFAAGTLRSLAARRANFSSAAAKWEGGVSMVQGASRGIGLQFVRHLLQKNEKGHVVAT 68
           +P  AA    S        +  + K EG V+++ G + G+G       L+   +      
Sbjct: 16  RPATAASAAYSTGGGGGGCTCTSKKLEGKVALITGGASGLGKATASEFLRHGAR----VV 75

Query: 69  CRNPGESTGLIELKDKFSERLCILELDVTKESTIKASAKSIEERYGSLNLLINASGILSI 128
             +    TG    K+  SE    +  DVT E+ I  + +   ERYG L+++ N +GI+  
Sbjct: 76  IADLDAETGTKTAKELGSEAE-FVRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIV-- 135

Query: 129 PGVLQPETTLTKVEKTSLLHAYEVNAVGPILVIKHLWPFLKAGGGSGTERDVAVVANLSA 188
            G + P  ++++++ T       +N  G +  IKH   F+     SG     + VA ++ 
Sbjct: 136 -GPMTP-ASISQLDMTEFERVMRINVFGVVSGIKHAAKFM-IPARSGCILCTSSVAGVTG 195

Query: 189 RVGSIGDNCLGGWHSYRASKTALNQLTKNVSVEFARKKDPIICILLHPGTVDTDLSRPFQ 248
            +           HSY  SK     + K+ + E       I CI   PGTV T L+  + 
Sbjct: 196 GLAP---------HSYTISKFTTPGIVKSAASELCEHGVRINCI--SPGTVATPLTLSYL 237

Query: 249 RNV 252
           + V
Sbjct: 256 QKV 237

BLAST of MS013393 vs. TAIR 10
Match: AT4G23430.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-08
Identity = 62/230 (26.96%), Postives = 99/230 (43.04%), Query Frame = 0

Query: 36  GGVSMVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIE--LKDKFSERLCILE 95
           G  ++V GAS GIG++  R L  +    HVV   RN      + E  +K     +L ++E
Sbjct: 29  GLTAIVTGASSGIGVETARVLSLRGV--HVVMAVRNTDSGAKVKEDIVKQVPGAKLDVME 88

Query: 96  LDVTKESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVN 155
           LD++   +++  A   +     LNLLIN +GI++ P +L          K ++   +  N
Sbjct: 89  LDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPFML---------SKDNIELQFATN 148

Query: 156 AVGPILVIKHLWPFLKAGGGSGTERDVAVVANLSA-----------RVGSIGD-NCLGGW 215
            +G  L+ K L   +K+   S   +    + NLS+           R   I D +     
Sbjct: 149 HLGHFLLTKLLLDTMKS--TSRESKREGRIVNLSSEAHRFSYPEGVRFDKINDKSSYSSM 208

Query: 216 HSYRASKTA----LNQLTKNVSVEFARKKDPIICILLHPGTVDTDLSRPF 248
            +Y  SK       N+LTK +  +       I    LHPG + T+L R F
Sbjct: 209 RAYGQSKLCNVLHANELTKQLKEDGVN----ITANSLHPGAIMTNLGRYF 241

BLAST of MS013393 vs. TAIR 10
Match: AT4G23430.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 62/229 (27.07%), Postives = 98/229 (42.79%), Query Frame = 0

Query: 36  GGVSMVQGASRGIGLQFVRHLLQKNEKGHVVATCRNPGESTGLIE--LKDKFSERLCILE 95
           G  ++V GAS GIG++  R L  +    HVV   RN      + E  +K     +L ++E
Sbjct: 29  GLTAIVTGASSGIGVETARVLSLRGV--HVVMAVRNTDSGAKVKEDIVKQVPGAKLDVME 88

Query: 96  LDVTKESTIKASAKSIEERYGSLNLLINASGILSIPGVLQPETTLTKVEKTSLLHAYEVN 155
           LD++   +++  A   +     LNLLIN +GI++ P +L          K ++   +  N
Sbjct: 89  LDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPFML---------SKDNIELQFATN 148

Query: 156 AVGPILVIKHLWPFLKAGGGSGTERDVAVVANLSA-----------RVGSIGDNCLGGWH 215
            +G  L+ K L   +K+   S   +    + NLS+           R   I D       
Sbjct: 149 HLGHFLLTKLLLDTMKS--TSRESKREGRIVNLSSEAHRFSYPEGVRFDKINDKS-SSMR 208

Query: 216 SYRASKTA----LNQLTKNVSVEFARKKDPIICILLHPGTVDTDLSRPF 248
           +Y  SK       N+LTK +  +       I    LHPG + T+L R F
Sbjct: 209 AYGQSKLCNVLHANELTKQLKEDGVN----ITANSLHPGAIMTNLGRYF 239