MS013247 (gene) Bitter gourd (TR) v1

Overview
NameMS013247
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMono-/di-acylglycerol lipase, N-terminal;Lipase, class 3
Locationscaffold459: 1528789 .. 1533286 (+)
RNA-Seq ExpressionMS013247
SyntenyMS013247
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCTGCTGCACTTCTATATTATACATTGAACCGTAAATTGCAGTCAAGCAGAGATCAAGGGGATGGAGATGTCAATAGTAATGACACTTCCAGTCATGCTCTTCTGGGAGGTGATCGAGTTTCCCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCAGAGACCCTTAGGTTTACGTACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAACTTTCTCTTAAAGAGACAGGTATGGAGCTTTTGGATGGTTGTAGTTTTTTAAGGTATTAAATGTAGCCTATTATATGTAAATTGTAATGTAACAGGCTGGTTGGTTTTTTTCCTTCACTATGTCTTCCTTCAAAAATGATGGTACTGATATTTAATCTGGTTCCTTAAAGGTGTATCTGCTTTGAAGTGTGAGTTTTGTTTTCTATCACTTTTGGTGTACACGATAATAGTGGTCTACTGGTCTTCACTTAATTTGGTTTCATTCCTCTAAATACGCAAAATCTTATATGATGAGCAATTATCCTCTAGTTGAATTTTATTGTTATCTTTAATATATCTACTTAGAGGTAAAGGAAGGCCTAAATTCTTCTATTTTGTGGTCTATAAACATATATATTTTTTATTATATTATCTATTTGTGGTCTATTTATATGACTTTCTGGAAGAAGAAAAGAGGTGAGTTGATTATATTATCTTGGTATACCAAGGGTCTTGGAGTTTTTGGTTTTAAGGGAAAAGGTAGGATGCTTTATCAAATTTTCCTCGGAAATGCTTATGTTATGGTGGTTGGGATGCTTTCTTGTTGAGTTTGGAGAAAAATGACATTGGAAATATTGGAGTTTCACCTTCTCAAAACAAACCAGGAGTTATTCTTCAAAATTTTCTCGTTTCTTATGGTGGGTATGGTGGATTACTGGAGAAAATTCAGTTTTTTTTTAATCTTGTATTTATCAAAAGGATCAATCAGTTTAGAGTCTTGCTTTAGTGGTGATTAGAGAAATTTGAATTAAGGAGGAAGATAATAGCTATTAGCTTGATCAATTCCATATGAAGTTGTTGATCGGATGTGGTTTGTAGAATTGGGAGGGTGGGGATGTTATTTTTAGGGTACAATGTACATATTTTAGAGTGGAAGTAGTTTTAAAGGGGATGCTGTTGTAATTTCCTTGAAGAAGATTTCTTTTTGTTGGGGTACTCTGTTGTAATACTTCTAAAGGAAGATATATCCTTTTTCTTTATTGCCACTTTTCAGACATCAATGAGTTCTTGTTTACTTTATATAAAAAATCCTCAATATAAGAAGATAATGCTTCATCTGAACTATTCAAATCAATGGCAAATGTTGCTGGTTTGGTCTTTGATGGTATTTCATCAATGTGCAGGGAAACTTACATGTAGGCAGTGTATTTGGCAATGAAGGCAGTATTCAGCTTGAGGGAACTGAAATAATTTCTGAGCTGAAGTACCTCTTGCATTTGTTGACTTTGTGCTGGCACTTCTCAAAAAAGCCATTTCCGCTGTTTCTAGAAGAGACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAGTAAGTCATGATGAACTTCGGATAAATCACTCTCTATTGAAATAGATATTCTAATACTCGCCCTCACTTCTGTGTTTAATTTATCTTTAACATTATCACATCTATCCATGATTGCAGATTTTGAAGCCTGCTTTTACCATTATAGTAGACCATACAACAAAATGTATTCTTCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCATAGTGTTGTGCATGAGGGAGGGGTTAGCAATTTAGTTCTAGGATATGCACATTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTATCTACTCCTTGTCTTTTGAAAGCACTTGGTCAATATTCTGGTTATAATATTAAGGTTTGACATTTCTTGTTAGTTTACCGTGATGCTTTGCAACCAATAAAAATATAAGGGAAGCTTGATGGAATAATCTTGTGTTGGATTCTAATAGGTTGTGGGGCACTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAATTATCTATCACTTCTTGTGTTACATTTGCCCCAGGTTTGTATTATTTTTGCTGGTATCTCTGGGTTGTTTTTGACTATGGGCATTGTGCTTGGACATGATGTTCTTATTTCTTTCTATGAAGCTATCAAGTATCACGTTACTGAATATTTGTAACCTCTGCAGCTGCTTGTATGACATGGGAACTAGCAGAATCAGGCAATGAATTTATCACTTCTGTTATTAATGGAGCTGATTTGGTGCCGACCTTCTCAGCTGCTTCAGTAGATGACTTGCGCGCTGAGGTATGTTACAAATCCATTTAATTATTAAACAAATTTATTTGCTTTAAGTTTATCAAAGAGGTGACTACTCCATGAATTAAGTGTCTTTTTCTCTGAAGATTTTCAAATTATATTTTATCTTTTGGGGAAAGAAAATTTGAGTTAGAATTTAGTAGTGAATATTTATGGTTCATTTTATCATTTCTTGTTCTCCCCCAAAAATTGCACGTATTCCTTTTGAACATTTTTGCCTAGTAGAGTTGGTTTGCTGAGAATTCTTATGGATATATGAAAAAGGTTGGCTATTAAGATTTATAAAGTTGGAAAATGTCTGTGTACAACCAGCACGTTATTTCTTCTGCACTTAAAGGGGTCATCCATAGGTTATATTACTAAAACAATAGGTTGCCTGTAGGTAATACACATGCGTGTGTGTGTATATACACACAAACACATTATGTATGTATGGAAGAGGCATGAAATTTAGTTAACTTAAGCACTTCCCTCCAATCGTTTGCTGAAGCCGCTGAAATTTCTGTTATAGGTGACTGCTTCTGCCTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCTGCATTGGGATCCCGTCTCCCATCCATGGCCAGTGCTAGAGAAAAAGTTGCTGGTGCAGGGGCAATTCTGAGACCAGTCTCTAGTGGCACACAGGTAATGGCAATACGCATTGTACCCTTGTGATTGCTACGTATTAATAATTTTAGTAAAGCTGGACTTTGACAGAAACTTTGAGTGGATGTGATTTGGTTCTTCTCGCCTTCTCCTTCATAGCTGACTGAAACCTAATGCTGCTTGAAACCGTTTGGATAATGATTAGGCTTTAGGACCTCATGATTTTAGTTTAGCCTTCAAATAAACAAGGTATGCGATTGCCAATAAGCGGCCATTTGTTTTGCAGTAATCTTGGATTCCAGTGAGATGGATACCGAAACACTCGCAGATGCTCAGGCATCTTAGGATGTTCTTAGGCATCCTCATGCAATGTTTTTAGAAACAAACGGATGGACTCATCTTTCATAGTCATATAAAGATGTCAGATGTCCAGAAATAAATCCACGTTTTACGTCAAGCAATACATGGAGGAAGGGGTGGACATGTATTTCAATTTCTCGAAAATAAACGGCCCTAAATATTCTTGGAAGGTGTTATGTCGGGTTAATTCCCGTGTCTTGTGTATAATAATTAAATTTATATGTTATTGTGTTCTGTACTTCCGCAGGTTGTGATGAGGAGATGCCAGACCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGCCGTAGAGCCATGACTTCTCACTCAGTGGCGGAAGAAGGTGGAAATTCTCCAAAGTCATCTCCAAGAAAAACGGAATCCTGTGGGCCACTTAGATCACCCCCTCACGAAACTGTTGAAGCCATTGAACTTCCTGAATCTTCAGCTACAGGGATTCAGTGGACTAATGAAATTGAATGTTCCTATTCGGAGGAGATAAATCCTCATGGTATAGCAGAGGAGCCTGATGATGGTCAAGCCCTCATGGGCCATATTCAACATGAACAAATGACGGAAGTTGAGTTATGGCAGCAACTTGAGCATGAACTTTATGATAAGAGTGAGACTGATGTGGCCAAGGAAATAAGGGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGAGAGTCGGACGGGTCTGCGTCTGAAATGAAGGAAGCACACAGATTCTTTCCTGCAGGGAAGATCATGCACATTGTCGATATTCAATCAGATGCCGTTGATTGCGAAAGCGACAGCAGCTCCACGTCCAGCAATAGCCCATCAGCAGAGTCCAATATTGGTATTTTCCTTACACCAAGATCACTCTACAGCAAACTCAGATTGTCACAGACAATGATAAGTGACCACTACATGCCAGCTTACAGAAGACAGATTGAAAAGTTAATTAAAGAATTTGAAAAAGGAGAC

mRNA sequence

ATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCTGCTGCACTTCTATATTATACATTGAACCGTAAATTGCAGTCAAGCAGAGATCAAGGGGATGGAGATGTCAATAGTAATGACACTTCCAGTCATGCTCTTCTGGGAGGTGATCGAGTTTCCCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCAGAGACCCTTAGGTTTACGTACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAACTTTCTCTTAAAGAGACAGGGAAACTTACATGTAGGCAGTGTATTTGGCAATGAAGGCAGTATTCAGCTTGAGGGAACTGAAATAATTTCTGAGCTGAAGTACCTCTTGCATTTGTTGACTTTGTGCTGGCACTTCTCAAAAAAGCCATTTCCGCTGTTTCTAGAAGAGACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAATTTTGAAGCCTGCTTTTACCATTATAGTAGACCATACAACAAAATGTATTCTTCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCATAGTGTTGTGCATGAGGGAGGGGTTAGCAATTTAGTTCTAGGATATGCACATTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTATCTACTCCTTGTCTTTTGAAAGCACTTGGTCAATATTCTGGTTATAATATTAAGGTTGTGGGGCACTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAATTATCTATCACTTCTTGTGTTACATTTGCCCCAGCTGCTTGTATGACATGGGAACTAGCAGAATCAGGCAATGAATTTATCACTTCTGTTATTAATGGAGCTGATTTGGTGCCGACCTTCTCAGCTGCTTCAGTAGATGACTTGCGCGCTGAGGTGACTGCTTCTGCCTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCTGCATTGGGATCCCGTCTCCCATCCATGGCCAGTGCTAGAGAAAAAGTTGCTGGTGCAGGGGCAATTCTGAGACCAGTCTCTAGTGGCACACAGGTTGTGATGAGGAGATGCCAGACCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGCCGTAGAGCCATGACTTCTCACTCAGTGGCGGAAGAAGGTGGAAATTCTCCAAAGTCATCTCCAAGAAAAACGGAATCCTGTGGGCCACTTAGATCACCCCCTCACGAAACTGTTGAAGCCATTGAACTTCCTGAATCTTCAGCTACAGGGATTCAGTGGACTAATGAAATTGAATGTTCCTATTCGGAGGAGATAAATCCTCATGGTATAGCAGAGGAGCCTGATGATGGTCAAGCCCTCATGGGCCATATTCAACATGAACAAATGACGGAAGTTGAGTTATGGCAGCAACTTGAGCATGAACTTTATGATAAGAGTGAGACTGATGTGGCCAAGGAAATAAGGGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGAGAGTCGGACGGGTCTGCGTCTGAAATGAAGGAAGCACACAGATTCTTTCCTGCAGGGAAGATCATGCACATTGTCGATATTCAATCAGATGCCGTTGATTGCGAAAGCGACAGCAGCTCCACGTCCAGCAATAGCCCATCAGCAGAGTCCAATATTGGTATTTTCCTTACACCAAGATCACTCTACAGCAAACTCAGATTGTCACAGACAATGATAAGTGACCACTACATGCCAGCTTACAGAAGACAGATTGAAAAGTTAATTAAAGAATTTGAAAAAGGAGAC

Coding sequence (CDS)

ATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCTGCTGCACTTCTATATTATACATTGAACCGTAAATTGCAGTCAAGCAGAGATCAAGGGGATGGAGATGTCAATAGTAATGACACTTCCAGTCATGCTCTTCTGGGAGGTGATCGAGTTTCCCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCAGAGACCCTTAGGTTTACGTACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAACTTTCTCTTAAAGAGACAGGGAAACTTACATGTAGGCAGTGTATTTGGCAATGAAGGCAGTATTCAGCTTGAGGGAACTGAAATAATTTCTGAGCTGAAGTACCTCTTGCATTTGTTGACTTTGTGCTGGCACTTCTCAAAAAAGCCATTTCCGCTGTTTCTAGAAGAGACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAATTTTGAAGCCTGCTTTTACCATTATAGTAGACCATACAACAAAATGTATTCTTCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCATAGTGTTGTGCATGAGGGAGGGGTTAGCAATTTAGTTCTAGGATATGCACATTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTATCTACTCCTTGTCTTTTGAAAGCACTTGGTCAATATTCTGGTTATAATATTAAGGTTGTGGGGCACTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAATTATCTATCACTTCTTGTGTTACATTTGCCCCAGCTGCTTGTATGACATGGGAACTAGCAGAATCAGGCAATGAATTTATCACTTCTGTTATTAATGGAGCTGATTTGGTGCCGACCTTCTCAGCTGCTTCAGTAGATGACTTGCGCGCTGAGGTGACTGCTTCTGCCTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCTGCATTGGGATCCCGTCTCCCATCCATGGCCAGTGCTAGAGAAAAAGTTGCTGGTGCAGGGGCAATTCTGAGACCAGTCTCTAGTGGCACACAGGTTGTGATGAGGAGATGCCAGACCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGCCGTAGAGCCATGACTTCTCACTCAGTGGCGGAAGAAGGTGGAAATTCTCCAAAGTCATCTCCAAGAAAAACGGAATCCTGTGGGCCACTTAGATCACCCCCTCACGAAACTGTTGAAGCCATTGAACTTCCTGAATCTTCAGCTACAGGGATTCAGTGGACTAATGAAATTGAATGTTCCTATTCGGAGGAGATAAATCCTCATGGTATAGCAGAGGAGCCTGATGATGGTCAAGCCCTCATGGGCCATATTCAACATGAACAAATGACGGAAGTTGAGTTATGGCAGCAACTTGAGCATGAACTTTATGATAAGAGTGAGACTGATGTGGCCAAGGAAATAAGGGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGAGAGTCGGACGGGTCTGCGTCTGAAATGAAGGAAGCACACAGATTCTTTCCTGCAGGGAAGATCATGCACATTGTCGATATTCAATCAGATGCCGTTGATTGCGAAAGCGACAGCAGCTCCACGTCCAGCAATAGCCCATCAGCAGAGTCCAATATTGGTATTTTCCTTACACCAAGATCACTCTACAGCAAACTCAGATTGTCACAGACAATGATAAGTGACCACTACATGCCAGCTTACAGAAGACAGATTGAAAAGTTAATTAAAGAATTTGAAAAAGGAGAC

Protein sequence

MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPATWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGTEIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLSTPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASALGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPRRRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECSYSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEAAAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD
Homology
BLAST of MS013247 vs. NCBI nr
Match: XP_022148201.1 (uncharacterized protein LOC111016928 [Momordica charantia])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 639/641 (99.69%), Postives = 640/641 (99.84%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT
Sbjct: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS
Sbjct: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480

Query: 481 YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEAA 540
           YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYD+SETDVAKEIREEEAA
Sbjct: 481 YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYDESETDVAKEIREEEAA 540

Query: 541 AMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI 600
           AMAEVGESDGS SEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI
Sbjct: 541 AMAEVGESDGSVSEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI 600

Query: 601 FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD 642
           FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD
Sbjct: 601 FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD 641

BLAST of MS013247 vs. NCBI nr
Match: XP_004144431.1 (uncharacterized protein LOC101203983 [Cucumis sativus] >KGN58447.1 hypothetical protein Csa_002505 [Cucumis sativus])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 582/647 (89.95%), Postives = 603/647 (93.20%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SS DQ DGDV+ ND S+HALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNE SIQL+GT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+I+ELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDH T
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR+EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRSEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAILRPVSSGTQVVM+R Q+MAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIEL---PESSATGIQWTNEI 480
           RRAM SHSVAEEGG+SPK SPRK ESC PLRS P ETVEAIE    PESS T +QW+NEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480

Query: 481 ECSYSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIRE 540
           E SYSEEINP GI +E  DDGQ LMG+IQ EQMTEVELWQQLEHELYDK E DVA+EIRE
Sbjct: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSST----SSNSP 600
           EEAAAMAEVG+SD SA  +KEAHRFFPAGKIMH++DIQSDA DCESDSSS+    S NSP
Sbjct: 541 EEAAAMAEVGQSDTSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600

Query: 601 SAESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
            AE  IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKLIKE EK
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEK 647

BLAST of MS013247 vs. NCBI nr
Match: XP_038901142.1 (uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida] >XP_038901147.1 uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 579/645 (89.77%), Postives = 595/645 (92.25%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SS DQ DGDV+ NDT SHALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDTPSHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNE SIQL+GT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+I+ELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKAL QYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALAQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAILRPVSSGTQVVM+R Q+MAQAAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAM SHSVAEE G+SPK SPRK E C PLRS P E VEAIE PESS T +QWTNEIECS
Sbjct: 421 RRAMASHSVAEEDGSSPKPSPRKMEPCEPLRSSPEEIVEAIEHPESSTTAMQWTNEIECS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEI P G+ +   DDGQALM HIQ EQMTEVELWQQLEHELYD+ E DVAKEIREEEA
Sbjct: 481 YSEEIIPEGMTDGLDDDGQALMDHIQDEQMTEVELWQQLEHELYDRGEPDVAKEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSS---NSPSAES 600
           AAMA VG+SD S S +KEAHRFFPAGKIMHI+DIQSD+  CESDSSS SS   NSP  ES
Sbjct: 541 AAMAAVGQSDSSTSGIKEAHRFFPAGKIMHIIDIQSDSPVCESDSSSASSISDNSPLEES 600

Query: 601 NIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD 642
            IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKLIKE EK D
Sbjct: 601 KIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKED 645

BLAST of MS013247 vs. NCBI nr
Match: XP_008460311.1 (PREDICTED: uncharacterized protein LOC103499170 [Cucumis melo])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 576/644 (89.44%), Postives = 597/644 (92.70%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SS DQ DGDV+ ND  +HALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNE SIQL+G 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+I+ELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAILRPVSSGTQVVM+R Q+MA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAM SHSVAEEGG+SPK SPRK ESC PLRS P E VEAIE  ESS T ++W+NEIE S
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIVEAIEPTESSTTAMEWSNEIEYS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEINP GI +E  DDGQ LM +IQ EQMTEVELWQQLEHELYDK E DVA+EIREEEA
Sbjct: 481 YSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSS----NSPSAE 600
           AAMAEVG+SD SAS +KEAHRFFPAGKIMHI+DIQSDA DCESDSSS++S    NSP AE
Sbjct: 541 AAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSPLAE 600

Query: 601 SNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
             IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQ+EKLIKE EK
Sbjct: 601 CKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQVEKLIKELEK 644

BLAST of MS013247 vs. NCBI nr
Match: XP_022956309.1 (uncharacterized protein LOC111458044 [Cucurbita moschata])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 574/645 (88.99%), Postives = 595/645 (92.25%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SSRDQ D DV  ND SSHALLGGDRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGGDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNE S QL+G 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+ISELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAIL+PVSSGTQVVM+R Q+MAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAMTSHS AEE G+SPKSSPRK ES  PLRS P E VEA ELPESS T IQWTNEIECS
Sbjct: 421 RRAMTSHSTAEENGSSPKSSPRKMESFEPLRSSPQEIVEATELPESSTTTIQWTNEIECS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEINP G+ +E  DD QALM HIQ EQ+TEVELWQQLEHEL+D+SE DVAKEIREEEA
Sbjct: 481 YSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDI--QSDAVDCESDS---SSTSSNSPSA 600
           AAMAEVG+SD   S MKEAHRFFPAGKIMHI++I  QSDA DCESDS   SSTS +SP A
Sbjct: 541 AAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIEIETQSDAPDCESDSSSRSSTSDSSPQA 600

Query: 601 ESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
           +S  GIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKL+KE EK
Sbjct: 601 QSRTGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVKELEK 645

BLAST of MS013247 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 78.6 bits (192), Expect = 2.9e-13
Identity = 59/178 (33.15%), Postives = 87/178 (48.88%), Query Frame = 0

Query: 167 ILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGAV----VPFHHSVVHEGGVSNLVL 226
           + +  F + VDH  K +++ IRGT S KD LT  TG      V  HH             
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGT----------- 436

Query: 227 GYAHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYIL 286
              H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++L
Sbjct: 437 WLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLL 496

Query: 287 REQKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRAEV 333
           R Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 RPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of MS013247 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 8.5e-13
Identity = 59/176 (33.52%), Postives = 88/176 (50.00%), Query Frame = 0

Query: 167 ILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGAV--VPFHHSVVHEGGVSNLVLGY 226
           + +  F + VDH  K +++ IRGT S KD LT  TG    +P      H G         
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEG---HRG------TWL 436

Query: 227 AHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYILRE 286
            H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++LR 
Sbjct: 437 GHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRP 496

Query: 287 QKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRAEV 333
           Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 Q--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of MS013247 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 8.5e-13
Identity = 59/176 (33.52%), Postives = 88/176 (50.00%), Query Frame = 0

Query: 167 ILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGAV--VPFHHSVVHEGGVSNLVLGY 226
           + +  F + VDH  K +++ IRGT S KD LT  TG    +P      H G         
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEG---HRG------TWL 436

Query: 227 AHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYILRE 286
            H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++LR 
Sbjct: 437 GHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRP 496

Query: 287 QKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRAEV 333
           Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 Q--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of MS013247 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.2e-12
Identity = 52/191 (27.23%), Postives = 92/191 (48.17%), Query Frame = 0

Query: 144 FPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGA 203
           F   L+ TG    + +       + +  F +++DH  + +++ +RGT S++D LT  +  
Sbjct: 330 FASILKTTGLQYRDFIHVSFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE 389

Query: 204 VVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAK--LSTPCLLKALGQYSGYNIKVVGH 263
                  +  +  V       AH G+  AAR+I +  ++   L +A      Y + VVGH
Sbjct: 390 SENLELDIELQDCV-------AHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGH 449

Query: 264 SLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFS 323
           SLG G AALL  +LR      + +     P   ++  L E   +F+ S+I G D++P  S
Sbjct: 450 SLGAGAAALLAIMLRGAYP-QVRAYAFSPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLS 509

Query: 324 AASVDDLRAEV 333
            A+++DL+  +
Sbjct: 510 VANMEDLKRRI 512

BLAST of MS013247 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-11
Identity = 53/191 (27.75%), Postives = 93/191 (48.69%), Query Frame = 0

Query: 144 FPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTTKCILLLIRGTHSIKDTLTAATGA 203
           F   L+ TG    + +       + +  F +++DH  + +++ +RGT S++D LT  +  
Sbjct: 330 FASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSA- 389

Query: 204 VVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAK--LSTPCLLKALGQYSGYNIKVVGH 263
                 S   E G+  L    AH G+  AAR+I +  ++   L +A      Y + +VGH
Sbjct: 390 -----ESETLELGI-ELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGH 449

Query: 264 SLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFS 323
           SLG G AALL  +LR      + +     P   ++  L E   +F+ S+I G D++P  S
Sbjct: 450 SLGAGAAALLAIMLRGAYP-QVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLS 509

Query: 324 AASVDDLRAEV 333
             +++DL+  +
Sbjct: 510 VTNMEDLKRRI 512

BLAST of MS013247 vs. ExPASy TrEMBL
Match: A0A6J1D4N7 (uncharacterized protein LOC111016928 OS=Momordica charantia OX=3673 GN=LOC111016928 PE=4 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 639/641 (99.69%), Postives = 640/641 (99.84%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT
Sbjct: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS
Sbjct: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480

Query: 481 YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEAA 540
           YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYD+SETDVAKEIREEEAA
Sbjct: 481 YSEEINPHGIAEEPDDGQALMGHIQHEQMTEVELWQQLEHELYDESETDVAKEIREEEAA 540

Query: 541 AMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI 600
           AMAEVGESDGS SEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI
Sbjct: 541 AMAEVGESDGSVSEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSSNSPSAESNIGI 600

Query: 601 FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD 642
           FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD
Sbjct: 601 FLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEKGD 641

BLAST of MS013247 vs. ExPASy TrEMBL
Match: A0A0A0LCM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G644860 PE=4 SV=1)

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 582/647 (89.95%), Postives = 603/647 (93.20%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SS DQ DGDV+ ND S+HALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNE SIQL+GT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+I+ELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDH T
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR+EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRSEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAILRPVSSGTQVVM+R Q+MAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIEL---PESSATGIQWTNEI 480
           RRAM SHSVAEEGG+SPK SPRK ESC PLRS P ETVEAIE    PESS T +QW+NEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480

Query: 481 ECSYSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIRE 540
           E SYSEEINP GI +E  DDGQ LMG+IQ EQMTEVELWQQLEHELYDK E DVA+EIRE
Sbjct: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSST----SSNSP 600
           EEAAAMAEVG+SD SA  +KEAHRFFPAGKIMH++DIQSDA DCESDSSS+    S NSP
Sbjct: 541 EEAAAMAEVGQSDTSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600

Query: 601 SAESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
            AE  IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKLIKE EK
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEK 647

BLAST of MS013247 vs. ExPASy TrEMBL
Match: A0A1S3CC71 (uncharacterized protein LOC103499170 OS=Cucumis melo OX=3656 GN=LOC103499170 PE=4 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 576/644 (89.44%), Postives = 597/644 (92.70%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SS DQ DGDV+ ND  +HALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNE SIQL+G 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+I+ELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAILRPVSSGTQVVM+R Q+MA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAM SHSVAEEGG+SPK SPRK ESC PLRS P E VEAIE  ESS T ++W+NEIE S
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIVEAIEPTESSTTAMEWSNEIEYS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEINP GI +E  DDGQ LM +IQ EQMTEVELWQQLEHELYDK E DVA+EIREEEA
Sbjct: 481 YSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDSSSTSS----NSPSAE 600
           AAMAEVG+SD SAS +KEAHRFFPAGKIMHI+DIQSDA DCESDSSS++S    NSP AE
Sbjct: 541 AAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSPLAE 600

Query: 601 SNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
             IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQ+EKLIKE EK
Sbjct: 601 CKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQVEKLIKELEK 644

BLAST of MS013247 vs. ExPASy TrEMBL
Match: A0A6J1GYN5 (uncharacterized protein LOC111458044 OS=Cucurbita moschata OX=3662 GN=LOC111458044 PE=4 SV=1)

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 574/645 (88.99%), Postives = 595/645 (92.25%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SSRDQ D DV  ND SSHALLGGDRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGGDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNE S QL+G 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+ISELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAIL+PVSSGTQVVM+R Q+MAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAMTSHS AEE G+SPKSSPRK ES  PLRS P E VEA ELPESS T IQWTNEIECS
Sbjct: 421 RRAMTSHSTAEENGSSPKSSPRKMESFEPLRSSPQEIVEATELPESSTTTIQWTNEIECS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEINP G+ +E  DD QALM HIQ EQ+TEVELWQQLEHEL+D+SE DVAKEIREEEA
Sbjct: 481 YSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDI--QSDAVDCESDS---SSTSSNSPSA 600
           AAMAEVG+SD   S MKEAHRFFPAGKIMHI++I  QSDA DCESDS   SSTS +SP A
Sbjct: 541 AAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIEIETQSDAPDCESDSSSRSSTSDSSPQA 600

Query: 601 ESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
           +S  GIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKL+KE EK
Sbjct: 601 QSRTGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVKELEK 645

BLAST of MS013247 vs. ExPASy TrEMBL
Match: A0A6J1IRP8 (uncharacterized protein LOC111479893 OS=Cucurbita maxima OX=3661 GN=LOC111479893 PE=4 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 571/643 (88.80%), Postives = 593/643 (92.22%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL SSRDQ D DV  ND SSHALLG DRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGVDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNE S QL+G 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+ISELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTIIVDH T
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+ASAR KVAGAGAIL+PVSSGTQVVM+R Q+MAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRAM SHS AEE G+SPKSSPRK ES  PLRS P E +EA ELPESS T IQWTNEIECS
Sbjct: 421 RRAMPSHSTAEENGSSPKSSPRKMESSEPLRSSPQEIIEATELPESSTTTIQWTNEIECS 480

Query: 481 YSEEINPHGIAEE-PDDGQALMGHIQHEQMTEVELWQQLEHELYDKSETDVAKEIREEEA 540
           YSEEINP G+ +E  DD QALM HIQ EQ+TEVELWQQLEHEL+D+SE DVAKEIREEEA
Sbjct: 481 YSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIREEEA 540

Query: 541 AAMAEVGESDGSASEMKEAHRFFPAGKIMHIVDIQSDAVDCESDS---SSTSSNSPSAES 600
           AAMAEVG+SD   S MKEAHRFFPAGKIMHI+D QSDA DCESDS   SSTS +SP A+S
Sbjct: 541 AAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIDTQSDAPDCESDSSSRSSTSDSSPQAQS 600

Query: 601 NIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKEFEK 640
            IGIFLT RSLYSKLRLSQTMISDHYMPAYRRQIEKL++E EK
Sbjct: 601 RIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVRELEK 643

BLAST of MS013247 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 803.1 bits (2073), Expect = 1.6e-232
Identity = 441/651 (67.74%), Postives = 511/651 (78.49%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL +     D D NS  ++S   L  DRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVD-DENSEASASRPSLRIDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFL+KRQG LHV  VFG + S++L G+
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGS 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+ +ELKYLLHLLTLCWHFSKK FP FLEETGF+KENVL+ EPKAGILKPAFT++VDH T
Sbjct: 121 EVATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           K  LLLIRGTHSIKDTLTAATGA+VPFHH+VV+E GVSNLVLGYAHCGMVAAAR IAKL+
Sbjct: 181 KYFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLA 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLK L QY  Y IK+VGHSLGGGTAALLTYI+REQK LS  +CVTFAPAACMTWELA
Sbjct: 241 TPCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           +SGN+FI SVINGADLVPTFSAA+VDDLRAEVTASAW+NDLRNQIE TRILSTVYRSA+A
Sbjct: 301 DSGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSMA+A+ KVAGAGA+LRPVSSGTQVVMRR Q+M     TRP+L +SSWSC+GPR
Sbjct: 361 LGSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSML----TRPALSISSWSCMGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRA  + S++E           + ++   +     ET + + + +   TG +W +E ECS
Sbjct: 421 RRASATQSISE----------HQLDTSEAMSQDIPETSDPLLVTDEEITG-KWKSEAECS 480

Query: 481 YSEEINPHGIAEEPDDGQ-ALMGHIQHEQMTEVELWQQLEHELYDKS-----ETDVAKEI 540
             EE +P   A + D+ +       + E+MTE ELWQQLEH+LY  S     ETDVAKEI
Sbjct: 481 NYEETSPRLGATDLDECEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQPEETDVAKEI 540

Query: 541 REEEAAAMAEVGES--DGSASEMKEAHRFFPAGKIMHIVDIQSDAVDC--ESDSSSTSSN 600
           +EEE A +AE G +  +   +EMKE+ RF PAGKIMHIV ++ +AV+   E D   ++  
Sbjct: 541 KEEEEAVIAEAGVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALE 600

Query: 601 SPSA-----ESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKE 637
            P       E  +GIFLTPRSLYSK+RLSQ MISDH+MP YRRQIE+LI+E
Sbjct: 601 RPETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQE 635

BLAST of MS013247 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 803.1 bits (2073), Expect = 1.6e-232
Identity = 441/651 (67.74%), Postives = 511/651 (78.49%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLQSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL +     D D NS  ++S   L  DRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLIAGPSDVD-DENSEASASRPSLRIDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFL+KRQG LHV  VFG + S++L G+
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGS 120

Query: 121 EIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTT 180
           E+ +ELKYLLHLLTLCWHFSKK FP FLEETGF+KENVL+ EPKAGILKPAFT++VDH T
Sbjct: 121 EVATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           K  LLLIRGTHSIKDTLTAATGA+VPFHH+VV+E GVSNLVLGYAHCGMVAAAR IAKL+
Sbjct: 181 KYFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLA 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLK L QY  Y IK+VGHSLGGGTAALLTYI+REQK LS  +CVTFAPAACMTWELA
Sbjct: 241 TPCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           +SGN+FI SVINGADLVPTFSAA+VDDLRAEVTASAW+NDLRNQIE TRILSTVYRSA+A
Sbjct: 301 DSGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATA 360

Query: 361 LGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSMA+A+ KVAGAGA+LRPVSSGTQVVMRR Q+M     TRP+L +SSWSC+GPR
Sbjct: 361 LGSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSML----TRPALSISSWSCMGPR 420

Query: 421 RRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEIECS 480
           RRA  + S++E           + ++   +     ET + + + +   TG +W +E ECS
Sbjct: 421 RRASATQSISE----------HQLDTSEAMSQDIPETSDPLLVTDEEITG-KWKSEAECS 480

Query: 481 YSEEINPHGIAEEPDDGQ-ALMGHIQHEQMTEVELWQQLEHELYDKS-----ETDVAKEI 540
             EE +P   A + D+ +       + E+MTE ELWQQLEH+LY  S     ETDVAKEI
Sbjct: 481 NYEETSPRLGATDLDECEDPAEMDTREERMTEAELWQQLEHDLYHDSSEQPEETDVAKEI 540

Query: 541 REEEAAAMAEVGES--DGSASEMKEAHRFFPAGKIMHIVDIQSDAVDC--ESDSSSTSSN 600
           +EEE A +AE G +  +   +EMKE+ RF PAGKIMHIV ++ +AV+   E D   ++  
Sbjct: 541 KEEEEAVIAEAGVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEEDEDGSALE 600

Query: 601 SPSA-----ESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKE 637
            P       E  +GIFLTPRSLYSK+RLSQ MISDH+MP YRRQIE+LI+E
Sbjct: 601 RPETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQE 635

BLAST of MS013247 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 622.1 bits (1603), Expect = 5.0e-178
Identity = 360/666 (54.05%), Postives = 451/666 (67.72%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKL---QSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQ 60
           MA   M TA GA  +L Y L+R++   ++  D   G++  +  S     G  R+  R  Q
Sbjct: 1   MAAGVMVTATGAVVIL-YLLSRRIVWARNGEDDSGGELGKSGRS-----GRRRIVRRPAQ 60

Query: 61  APATWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQL 120
           APATWLETISTLSETLRFTYSETLGKWPI DLAFGIN+L++RQGN    SV+     I+L
Sbjct: 61  APATWLETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIEL 120

Query: 121 EGTEIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVD 180
           +G EII +L  LL  LTLC  FSKKPF +FLE  G++ E+VLLQ+PKAGI++PAFTII D
Sbjct: 121 KGPEIIMDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRD 180

Query: 181 HTTKCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIA 240
             +KCILLLIRGTHSIKDTLTAATGAVVPFHHSV+H+GG+SNLVLGYAHCGMVAAARWIA
Sbjct: 181 TNSKCILLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIA 240

Query: 241 KLSTPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTW 300
           KLS PCLLKAL +   + +++VGHSLGGGTA+LLTYILREQKE +  +C TFAPAACMTW
Sbjct: 241 KLSVPCLLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAPAACMTW 300

Query: 301 ELAESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRS 360
           +LAESG  FIT++ING+DLVPTFSA+SVDDLR+EVT+S+W NDLR+Q+E TR+LS VYRS
Sbjct: 301 DLAESGKHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRS 360

Query: 361 ASALGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCI 420
           A+A+GSRLPS+ASA+ KVAGAGAILRPVSSGTQV+++R Q +AQA     S  LSSWSCI
Sbjct: 361 ATAIGSRLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRST-LSSWSCI 420

Query: 421 GPRRRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEI 480
           GPRRRA++S  +  +  + P++S    E     RS      E + +      G + T   
Sbjct: 421 GPRRRAISS-QLNSKVTDMPEASAIMAER----RSTEALLAETVAIDRK---GHKRTEHS 480

Query: 481 ECSYSEEINPHGIAEEPDDGQALMGHI------QHEQMTEVELWQQLEHELYDKSETDVA 540
             S SE        EE ++    +  +        E +TE ELW +L+ EL  + E +  
Sbjct: 481 SSSSSESDRDEPDEEEEEEPLISIDQVIAETSSIEEDVTEGELWDELDREL-TRQENERD 540

Query: 541 KEIREEEAAAMAEVGESD-------------------GSASEMKEAHRFFPAGKIMHIVD 600
            E  EEEAAA  E+ E +                    S+ ++ E  RF+P GKIMHIV 
Sbjct: 541 SEAMEEEAAAAKEITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVS 600

Query: 601 IQSDAVDCESDSSSTSSNSPSAESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEK 639
           +     + E D         +    + I+ TPR LY K+RLS+TMI+DHYMP Y++ +E 
Sbjct: 601 VTETESETERDEVVVVGT--TTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMEL 648

BLAST of MS013247 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 597.8 bits (1540), Expect = 1.0e-170
Identity = 353/666 (53.00%), Postives = 443/666 (66.52%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKL---QSSRDQGDGDVNSNDTSSHALLGGDRVSHRLIQ 60
           MA   M TA GA  +L Y L+R++   ++  D   G++  +  S     G  R+  R  Q
Sbjct: 1   MAAGVMVTATGAVVIL-YLLSRRIVWARNGEDDSGGELGKSGRS-----GRRRIVRRPAQ 60

Query: 61  APATWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQL 120
           APATWLETISTLSETLRFTYSETLGKWPI DLAFGIN+L++RQGN    SV+     I+L
Sbjct: 61  APATWLETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIEL 120

Query: 121 EGTEIISELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVD 180
           +G EII +L  LL  LTLC  FSKKPF +FLE  G++ E+VLLQ+PKAGI++PAFTII D
Sbjct: 121 KGPEIIMDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRD 180

Query: 181 HTTKCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIA 240
             +KCILLLIRGTHSIKDTLTAATGAVVPFHHSV+H+GG+SNLVLGYAHCGMVAAARWIA
Sbjct: 181 TNSKCILLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIA 240

Query: 241 KLSTPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTW 300
           KLS PCLLKAL +   + +++VGHSLGGGTA+LLTYILREQKE +  +C TFAP      
Sbjct: 241 KLSVPCLLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAP------ 300

Query: 301 ELAESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRS 360
             AESG  FIT++ING+DLVPTFSA+SVDDLR+EVT+S+W NDLR+Q+E TR+LS VYRS
Sbjct: 301 --AESGKHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRS 360

Query: 361 ASALGSRLPSMASAREKVAGAGAILRPVSSGTQVVMRRCQTMAQAAWTRPSLHLSSWSCI 420
           A+A+GSRLPS+ASA+ KVAGAGAILRPVSSGTQV+++R Q +AQA     S  LSSWSCI
Sbjct: 361 ATAIGSRLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRST-LSSWSCI 420

Query: 421 GPRRRAMTSHSVAEEGGNSPKSSPRKTESCGPLRSPPHETVEAIELPESSATGIQWTNEI 480
           GPRRRA++S  +  +  + P++S    E     RS      E + +      G + T   
Sbjct: 421 GPRRRAISS-QLNSKVTDMPEASAIMAER----RSTEALLAETVAIDRK---GHKRTEHS 480

Query: 481 ECSYSEEINPHGIAEEPDDGQALMGHI------QHEQMTEVELWQQLEHELYDKSETDVA 540
             S SE        EE ++    +  +        E +TE ELW +L+ EL  + E +  
Sbjct: 481 SSSSSESDRDEPDEEEEEEPLISIDQVIAETSSIEEDVTEGELWDELDREL-TRQENERD 540

Query: 541 KEIREEEAAAMAEVGESD-------------------GSASEMKEAHRFFPAGKIMHIVD 600
            E  EEEAAA  E+ E +                    S+ ++ E  RF+P GKIMHIV 
Sbjct: 541 SEAMEEEAAAAKEITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVS 600

Query: 601 IQSDAVDCESDSSSTSSNSPSAESNIGIFLTPRSLYSKLRLSQTMISDHYMPAYRRQIEK 639
           +     + E D         +    + I+ TPR LY K+RLS+TMI+DHYMP Y++ +E 
Sbjct: 601 VTETESETERDEVVVVGT--TTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKKMMEL 640

BLAST of MS013247 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 5.6e-28
Identity = 82/286 (28.67%), Postives = 137/286 (47.90%), Query Frame = 0

Query: 68  TLSETLRFTYSETLG--KWPIGDLAFGINFLLKRQGNLHVGSVFGNEGSIQLEGTEIISE 127
           +LSE +       LG   W  GDL  G+  +  RQ +L   S F +   +++     + +
Sbjct: 131 SLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASL---SPFEDVKGVEVVSESTVYD 190

Query: 128 LKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHTTKCILL 187
           L Y   L   C+  S        + T   + N+L     + +++P + I VDH  K ++ 
Sbjct: 191 LIYNAELAKGCYRDSVSG---LAKNTMLRENNILKFVKDSSVMRPGYYIGVDHRRKLVVF 250

Query: 188 LIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYA-HCGMVAAARWIAKLSTPCL 247
            IRGTH+I D +T            +V          GY+ H G   AARW        +
Sbjct: 251 GIRGTHTIYDLIT-----------DIVSSSDEEVTFEGYSTHFGTAEAARWFLNHELQTI 310

Query: 248 LKALGQYSGYNIKVVGHSLGGGTAALLTYILREQK------ELSITSCVTFAPAACMTWE 307
            + L +Y GY +++VGHSLGG  A+L+  +L++        +  I S V +A   C++ E
Sbjct: 311 RRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCVSKE 370

Query: 308 LAESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQ 345
           LAE+ +EF+T+++   D++P  SAAS+  LR E+  + W + +  +
Sbjct: 371 LAENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVIEKE 399

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148201.10.0e+0099.69uncharacterized protein LOC111016928 [Momordica charantia][more]
XP_004144431.10.0e+0089.95uncharacterized protein LOC101203983 [Cucumis sativus] >KGN58447.1 hypothetical ... [more]
XP_038901142.10.0e+0089.77uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida] >XP_03890114... [more]
XP_008460311.10.0e+0089.44PREDICTED: uncharacterized protein LOC103499170 [Cucumis melo][more]
XP_022956309.10.0e+0088.99uncharacterized protein LOC111458044 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9Y4D22.9e-1333.15Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3[more]
Q6WQJ18.5e-1333.52Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Q5YLM18.5e-1333.52Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1[more]
P0C1S94.2e-1227.23Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q91WC91.6e-1127.75Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D4N70.0e+0099.69uncharacterized protein LOC111016928 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A0A0LCM60.0e+0089.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G644860 PE=4 SV=1[more]
A0A1S3CC710.0e+0089.44uncharacterized protein LOC103499170 OS=Cucumis melo OX=3656 GN=LOC103499170 PE=... [more]
A0A6J1GYN50.0e+0088.99uncharacterized protein LOC111458044 OS=Cucurbita moschata OX=3662 GN=LOC1114580... [more]
A0A6J1IRP80.0e+0088.80uncharacterized protein LOC111479893 OS=Cucurbita maxima OX=3661 GN=LOC111479893... [more]
Match NameE-valueIdentityDescription
AT3G14075.11.6e-23267.74Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.21.6e-23267.74Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.15.0e-17854.05Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.21.0e-17053.00Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT2G42450.15.6e-2828.67alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 149..354
e-value: 1.8E-30
score: 108.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 138..322
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 185..322
e-value: 2.7E-23
score: 82.4
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 50..130
e-value: 3.9E-19
score: 68.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 425..454
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 429..446
NoneNo IPR availablePANTHERPTHR46023:SF6LIPASE CLASS 3 FAMILY PROTEINcoord: 1..639
NoneNo IPR availablePANTHERPTHR46023LIPASE CLASS 3 PROTEIN-LIKEcoord: 1..639
NoneNo IPR availableCDDcd00519Lipase_3coord: 176..321
e-value: 8.1308E-37
score: 135.297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS013247.1MS013247.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process