MS012557 (gene) Bitter gourd (TR) v1

Overview
NameMS012557
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionChloride channel protein
Locationscaffold63: 1521140 .. 1526088 (+)
RNA-Seq ExpressionMS012557
SyntenyMS012557
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATTGAGAATGATCTTTTCAAGCAAGACTGGAGGTCCAGAACAAAGATTGAGATATTTCAGTATATTATCCTCAAATGGACACTGTGCCTTCTCATTGGTTTATTTACAGGGCTTGTTGGATTTTCCAACAACATAGCTGTTGAGAATATTGCTGGTGTGAAACTTCTGATGACCAACAGTCTCATGCTCAAGCAAAAGTAAGCTTTGTGTGACGCCCAGTATAAGAAGTATTTGATTCATATAGCATGATAGAACCTTACAAGGCCATGTTCAAGGCTTGAAATAGAATTTGTGATAATGAATGTTCCCTATGTCCCTTACAATTCAACATAAGTACTCTAATGAACTAATCATTATATTCAGTACGATTATTTATAACAATGTCAAGAAGGGTATAAATGAATAGATTAATTCTAGTCTGATGGTTGACACATTTAAGATTCGAGCAGATATAGTTTAAGAATTAATATAGTACTAATATATTAAAAAAAAAAGAAATTTTACGTTTGAATTATTGGAACAGCTTTTTCTTCTCTTTTAAATTAATTGTCCCCTTGTTATGTCTTCTTAAGGACTTAGTCTAAATTTGTGTGTAAGGGGAATTGTTAGAAGTAAATATAAATTAATCCCGATCGTGTATTTATTTTTTCTAAGACCAGGTAATTGAGCACTTTTTCCTCTTTTCCTGTCATGAAGTTTGAAGATTCATGCACCTTTTCTGCATCATTGTCATTCAGCTCACTATGTGGCATTGTTCTTTCACTGTAATTGATCTAAATGATCTCAAAATCTTATCTTGCTGCAAATTGTCCATATGGGTTCTATGATTTTTCTTGCTGTGTTTCTTTCTCAATGTACCCATTTTAGATTTATATGTCATTCATACATTTCTTGTATATTACACCAACTTTGAGATGACCATTAGCTTCAGTTCAGGTACTATCAGGCATTTGCAGTATATGTTGCTTCCAATGTTGTTTTAGCTGTTGCTGCTGCAGCCCTCTGTGCCTATATTGCTCCTGCTGCTGCAGGTTCTGGCATACCTGAGGTGAAAGCATACCTAAATGGTATAGATGCTTATTCTATATTGGCTCCAAGTACCTTATTTGTGAAGGTAAGCCTCAGTTTAGCACCATTCCGTTTTCTCTGTCTTTTAACACTAACAAATAAGTAGTTGCTTATGGTCTCGACCATTCAAGGAAAAGTCAGCATCATGTATAGTATAAGCATTTTGAATGACCTGGGATCCTATTCAAACAAATTGTTGTGTGGCTTTCTGGGTTTAAACTTGAAAGATTCATGAGCAGCAAGTCTTAGTTTTAATTTCTTGAATTTGGTAATATTTGGATGAATCTTATGGCAATGTCTTGGTGTCTTAATGAGCTGGAAAGATGATACACCCCTACAGAGATTCACTTCTGCCAGGCGGTCTAAGGAGTATGGTAGGAAGAAAAATAGCAAGGAATCAGGTGGTTAGATATAGATAAGTGAAGGATAGGGCATGTACCTAACTATGCCGAGTCGTATGAATAATGTGTTAGGAGTGGGTGGGAAGAGATCATGAATGAAATTATACTTTGATTTCTTGCTTTGAGTCCTATCAAAAAGATATTGTGAGTTGGGCGTTTTCTACACCAGAAGAAATGACATTTTTGTTTTGGGGGTTCTATTATTGATATTCCCCCCGGGAGGGGGGGGGGGGGTGTTGTTCTCTTATATACTAGGCTATATTACCTTAGTTGTTTGTTCCGTATGGCATAATTTATTGTCTCACTCACTGTTGCAGATAAACCAGCATGTATGATCTAAAAGGCTGTCTTCTTTAGTAATTACAATCTTTTAAGTGACCATCGATAATATTATAAACTTGAATTATAGGTAGTAGTCCAGAACCTCTATTGTGTTTATGGTCCTTTGGAACGTAATATCATCTTAAATCAGCAGGAAGTTCAGAAACTGCTAACCATTATGATGCTGAACTTTAAAACTTTTCCAATTGTGGAATTGATTGTTGGATATTATCCGGATAAAAGTCATAATATAGTTCTTATGGGTGGTTTAAAAAAGCATGTGCCAGTTACAAATAACCTTGCGGATTAACTTGGCAGATTTTTGGTTCAATATTTGCTGTTGCTGGTGGATTTGTTGTGGGTAAGGAAGGACCCATGGTTCATACTGGTGCATGCATTGCCTCATTACTAGGACAAGGAGGTTCTCGCAAATACCGCTTGACTTGGAAGTGGCTCAGATACTTCAAAAATGATAGGGACAGGCGGGATTTGATCACCTGTGGTGCAGGAGCTGGTGTAGCAGCTGCCTTCCGTGCTCCAGTTGGTGGCGTTCTCTTTGCACTTGAAGAAGCAGCTTCATGGTTCTCTCTCTCTCTCATTTGGCTGCTCAGTTTTAAGTCATATTATAGGGGACGTGAGAGAAAAACTTGATCTAAGAAAGAAAATCAAGAGTCAAAACTTACGTAGCTTAGTTTTCTTGCCTAATAGAGGAAAACTTGATTTGATTGAGATAAGATGCGAGAAGTTATGCTTGCGGACAAGTTAAGCTGGGTTTTGTATTTTTTGGCTTCCTCTGGTATCTTGATTTTACTGACTGTGTTGAACTGCTTTCATATCCATATGGAATTTATTTTGGTAAACAATAAGGTAAATTTGGAACGTTCTAAGGTTTTCCTAGTTCAGATCGTATTTACATTGAAAAGCTTTTGAGGTTTTTATCTTCTCTCTCCATGCATATATAGATAATCTGTGTCATCTTTCTGTCACAATCATTAGGTGGAGGAGTTCTCTTCTCTGGAGGACCTTTTTCACTACTGCTGTTGTAGCCGTTGTCTTGAGGGGTTTCATGGCAATTTGCCGAGGGGGAACATGTGGGTTATTTGGTGAAGGAGGTCTCATCATGTTCGACATCCATACAGAAAATTCCAATTATGGCACTCCAGATCTTATTGCAATTGTCTTACTTGGAGTTATTGGCGGTGTGTTCGGAAGCCTTTACAACTTCCTCGTGGATAAGGTCCTCCGAACCTACAGCATCATAAATGAGTAAGCTCCCGAGTTTGTCTTATTGACGATTATGGAAGAGTTAATTTCCTCCCATTTTTTACACCATATACAGTCCTGTTATTGTACGGCTTCGCTATACAGTTGAGTTTCCTTTGCAGGAGAGGTCCTGGATGGAAAGTTATCCTTGTTGTTGCCGTTTCCATATTGACAACCTGTGTCTCATTTGGTCTTCCTTGGCTCTCGCAATGTTTACCTTGCCCACCTGACTTGGACGATCAATGCCCAACCGTTGGTCGCTCGGGAAACTACAAGAACTTCCAATGCCCACCTGGCCATTACAATGATCTTGCTTCTTTATTTTTCACCACCAATGATGATGCCATCCGGAACCTGTTCACATCTGCTATTGACAAGCAATTTCAGCTCTCCTCACTTTTTATCTTCTTTGCTGCTATCTATTGTCTTGGCATTATTACTTATGGCATCGCTGTGCCCTCAGGGCTCTTCATACCAGTAATACTGGCAGGAGCTTCTTATGGTCGCATTGTAGGGAGATTATTTGGTTCGGTTGCTGATGCTGATCTTGATGTCAGTCTCTATGCCCTTCTTGGAGCTGCTTCATTTCTTGGTGGCACTATGAGAATGACTGTTTCCCTATGTGTCATACTCCTTGAGCTTACTAATAACCTTTTGATGCTCCCATTACTAATGCTTGTTCTTCTAATTTCAAAGACGGTGGCGGATTTTTTTAATAAAGGTGTCTATGATCAGATTGTGAAGATGAAGGGACTACCTTTTATGGAAGCCCATGCTGAACCATACATGAGGCAACTGATTGCTGGGGGCGTGGCTTCTGGTCCCTTAATTACACTCTCTGGGATCGAAAAGGTCGAAAACGTAATGCAAGCTTTGACAATGACAAACCACAATGGGTTTCCTGTAGTTGATGAACCGCCATTCTCGGACAGTTCAGAACTTTGCGGGCTTGTTCTGAGGTCTCATCTGCTTGTTTTGCTTAAAGGAAAAAAGTTTACAAAGCAAAGGGTGTCAATTAGATCAGGCATCATGGGAAGCTTCAAAGCACATGATTTTGCAAAAGCTGGTTCTGGCAAAGGGGTGAAACTGGAAGATTTGGACATTGACGAGGAGGAGATGGAAATGTTTGTTGATCTTCATCCCATTACAAATACGTCGCCCTACACCGTAGTAGAAACAATGTCGCTAGCTAAAGCAGCGATTCTCTTTCACGGGCTCGGCTTGAGACACTTGTTGGTAGTTCCAAAGACACCCGGGGTAAGATATGCTTTTCTAACAGTCTTACTATGATTTATGAGCAAATGCACTTTGTTTCTGTAGCATAACATATTTGATTTTAGTCACTCAATACACACACCATCTCTGCTTTATAATCAATGATATAATATAATGTTCACCAATGACAGAAACTTCCTGCATTTCATTCTTTGGGAAGGCAAGCTAGTTTGATCTCCTGGTTTATGCCTAGAATTATTAGAAGTTTGTAGAAGATCTGTTGAATTTAGGTTTAAATCCTAATATGATCATTTAACACGACATTGACACATCTTTTGTTCAATTACTTTATCCAAAGTTTTTAGTTTTTCAGACGTATTCAAATCATTCTGGGAGGATTAGCAATAGATACGAAAATATGACTAAAACCGAAATAGAACATTTAAAAGTTTAGGAAGTAAAATAAAATGTCTCATCTGCACAGGAGATACTAAAACTATCGATTTAAATTTTTGAATCTAGAGATGATTTAACACTTTCTTGTTATATTTTGAATCTAGTAATGGTTTAACACTCATCGATCTTTTGCAGAGGCCTCCAATCACTGGAATCCTTACACGTCACGACTTCATGCCCGAGCATGTTCTAGGACTTTACCCCCACCTCAACCCCCACAAG

mRNA sequence

ATTATTGAGAATGATCTTTTCAAGCAAGACTGGAGGTCCAGAACAAAGATTGAGATATTTCAGTATATTATCCTCAAATGGACACTGTGCCTTCTCATTGGTTTATTTACAGGGCTTGTTGGATTTTCCAACAACATAGCTGTTGAGAATATTGCTGGTGTGAAACTTCTGATGACCAACAGTCTCATGCTCAAGCAAAAGTACTATCAGGCATTTGCAGTATATGTTGCTTCCAATGTTGTTTTAGCTGTTGCTGCTGCAGCCCTCTGTGCCTATATTGCTCCTGCTGCTGCAGGTTCTGGCATACCTGAGGTGAAAGCATACCTAAATGGTATAGATGCTTATTCTATATTGGCTCCAAGTACCTTATTTGTGAAGATTTTTGGTTCAATATTTGCTGTTGCTGGTGGATTTGTTGTGGGTAAGGAAGGACCCATGGTTCATACTGGTGCATGCATTGCCTCATTACTAGGACAAGGAGGTTCTCGCAAATACCGCTTGACTTGGAAGTGGCTCAGATACTTCAAAAATGATAGGGACAGGCGGGATTTGATCACCTGTGGTGCAGGAGCTGGTGTAGCAGCTGCCTTCCGTGCTCCAGTTGGTGGCGTTCTCTTTGCACTTGAAGAAGCAGCTTCATGGTGGAGGAGTTCTCTTCTCTGGAGGACCTTTTTCACTACTGCTGTTGTAGCCGTTGTCTTGAGGGGTTTCATGGCAATTTGCCGAGGGGGAACATGTGGGTTATTTGGTGAAGGAGGTCTCATCATGTTCGACATCCATACAGAAAATTCCAATTATGGCACTCCAGATCTTATTGCAATTGTCTTACTTGGAGTTATTGGCGGTGTGTTCGGAAGCCTTTACAACTTCCTCGTGGATAAGGTCCTCCGAACCTACAGCATCATAAATGAGAGAGGTCCTGGATGGAAAGTTATCCTTGTTGTTGCCGTTTCCATATTGACAACCTGTGTCTCATTTGGTCTTCCTTGGCTCTCGCAATGTTTACCTTGCCCACCTGACTTGGACGATCAATGCCCAACCGTTGGTCGCTCGGGAAACTACAAGAACTTCCAATGCCCACCTGGCCATTACAATGATCTTGCTTCTTTATTTTTCACCACCAATGATGATGCCATCCGGAACCTGTTCACATCTGCTATTGACAAGCAATTTCAGCTCTCCTCACTTTTTATCTTCTTTGCTGCTATCTATTGTCTTGGCATTATTACTTATGGCATCGCTGTGCCCTCAGGGCTCTTCATACCAGTAATACTGGCAGGAGCTTCTTATGGTCGCATTGTAGGGAGATTATTTGGTTCGGTTGCTGATGCTGATCTTGATGTCAGTCTCTATGCCCTTCTTGGAGCTGCTTCATTTCTTGGTGGCACTATGAGAATGACTGTTTCCCTATGTGTCATACTCCTTGAGCTTACTAATAACCTTTTGATGCTCCCATTACTAATGCTTGTTCTTCTAATTTCAAAGACGGTGGCGGATTTTTTTAATAAAGGTGTCTATGATCAGATTGTGAAGATGAAGGGACTACCTTTTATGGAAGCCCATGCTGAACCATACATGAGGCAACTGATTGCTGGGGGCGTGGCTTCTGGTCCCTTAATTACACTCTCTGGGATCGAAAAGGTCGAAAACGTAATGCAAGCTTTGACAATGACAAACCACAATGGGTTTCCTGTAGTTGATGAACCGCCATTCTCGGACAGTTCAGAACTTTGCGGGCTTGTTCTGAGGTCTCATCTGCTTGTTTTGCTTAAAGGAAAAAAGTTTACAAAGCAAAGGGTGTCAATTAGATCAGGCATCATGGGAAGCTTCAAAGCACATGATTTTGCAAAAGCTGGTTCTGGCAAAGGGGTGAAACTGGAAGATTTGGACATTGACGAGGAGGAGATGGAAATGTTTGTTGATCTTCATCCCATTACAAATACGTCGCCCTACACCGTAGTAGAAACAATGTCGCTAGCTAAAGCAGCGATTCTCTTTCACGGGCTCGGCTTGAGACACTTGTTGGTAGTTCCAAAGACACCCGGGAGGCCTCCAATCACTGGAATCCTTACACGTCACGACTTCATGCCCGAGCATGTTCTAGGACTTTACCCCCACCTCAACCCCCACAAG

Coding sequence (CDS)

ATTATTGAGAATGATCTTTTCAAGCAAGACTGGAGGTCCAGAACAAAGATTGAGATATTTCAGTATATTATCCTCAAATGGACACTGTGCCTTCTCATTGGTTTATTTACAGGGCTTGTTGGATTTTCCAACAACATAGCTGTTGAGAATATTGCTGGTGTGAAACTTCTGATGACCAACAGTCTCATGCTCAAGCAAAAGTACTATCAGGCATTTGCAGTATATGTTGCTTCCAATGTTGTTTTAGCTGTTGCTGCTGCAGCCCTCTGTGCCTATATTGCTCCTGCTGCTGCAGGTTCTGGCATACCTGAGGTGAAAGCATACCTAAATGGTATAGATGCTTATTCTATATTGGCTCCAAGTACCTTATTTGTGAAGATTTTTGGTTCAATATTTGCTGTTGCTGGTGGATTTGTTGTGGGTAAGGAAGGACCCATGGTTCATACTGGTGCATGCATTGCCTCATTACTAGGACAAGGAGGTTCTCGCAAATACCGCTTGACTTGGAAGTGGCTCAGATACTTCAAAAATGATAGGGACAGGCGGGATTTGATCACCTGTGGTGCAGGAGCTGGTGTAGCAGCTGCCTTCCGTGCTCCAGTTGGTGGCGTTCTCTTTGCACTTGAAGAAGCAGCTTCATGGTGGAGGAGTTCTCTTCTCTGGAGGACCTTTTTCACTACTGCTGTTGTAGCCGTTGTCTTGAGGGGTTTCATGGCAATTTGCCGAGGGGGAACATGTGGGTTATTTGGTGAAGGAGGTCTCATCATGTTCGACATCCATACAGAAAATTCCAATTATGGCACTCCAGATCTTATTGCAATTGTCTTACTTGGAGTTATTGGCGGTGTGTTCGGAAGCCTTTACAACTTCCTCGTGGATAAGGTCCTCCGAACCTACAGCATCATAAATGAGAGAGGTCCTGGATGGAAAGTTATCCTTGTTGTTGCCGTTTCCATATTGACAACCTGTGTCTCATTTGGTCTTCCTTGGCTCTCGCAATGTTTACCTTGCCCACCTGACTTGGACGATCAATGCCCAACCGTTGGTCGCTCGGGAAACTACAAGAACTTCCAATGCCCACCTGGCCATTACAATGATCTTGCTTCTTTATTTTTCACCACCAATGATGATGCCATCCGGAACCTGTTCACATCTGCTATTGACAAGCAATTTCAGCTCTCCTCACTTTTTATCTTCTTTGCTGCTATCTATTGTCTTGGCATTATTACTTATGGCATCGCTGTGCCCTCAGGGCTCTTCATACCAGTAATACTGGCAGGAGCTTCTTATGGTCGCATTGTAGGGAGATTATTTGGTTCGGTTGCTGATGCTGATCTTGATGTCAGTCTCTATGCCCTTCTTGGAGCTGCTTCATTTCTTGGTGGCACTATGAGAATGACTGTTTCCCTATGTGTCATACTCCTTGAGCTTACTAATAACCTTTTGATGCTCCCATTACTAATGCTTGTTCTTCTAATTTCAAAGACGGTGGCGGATTTTTTTAATAAAGGTGTCTATGATCAGATTGTGAAGATGAAGGGACTACCTTTTATGGAAGCCCATGCTGAACCATACATGAGGCAACTGATTGCTGGGGGCGTGGCTTCTGGTCCCTTAATTACACTCTCTGGGATCGAAAAGGTCGAAAACGTAATGCAAGCTTTGACAATGACAAACCACAATGGGTTTCCTGTAGTTGATGAACCGCCATTCTCGGACAGTTCAGAACTTTGCGGGCTTGTTCTGAGGTCTCATCTGCTTGTTTTGCTTAAAGGAAAAAAGTTTACAAAGCAAAGGGTGTCAATTAGATCAGGCATCATGGGAAGCTTCAAAGCACATGATTTTGCAAAAGCTGGTTCTGGCAAAGGGGTGAAACTGGAAGATTTGGACATTGACGAGGAGGAGATGGAAATGTTTGTTGATCTTCATCCCATTACAAATACGTCGCCCTACACCGTAGTAGAAACAATGTCGCTAGCTAAAGCAGCGATTCTCTTTCACGGGCTCGGCTTGAGACACTTGTTGGTAGTTCCAAAGACACCCGGGAGGCCTCCAATCACTGGAATCCTTACACGTCACGACTTCATGCCCGAGCATGTTCTAGGACTTTACCCCCACCTCAACCCCCACAAG

Protein sequence

IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTNSLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAICRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYSIINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCPPGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRVSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK
Homology
BLAST of MS012557 vs. NCBI nr
Match: XP_022136814.1 (chloride channel protein CLC-c [Momordica charantia])

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN
Sbjct: 71  IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 130

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 131 SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 190

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 191 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 250

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI
Sbjct: 251 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 310

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS
Sbjct: 311 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 370

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP
Sbjct: 371 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 430

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF
Sbjct: 431 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 490

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 491 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 550

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI
Sbjct: 551 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 610

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           TLSGIEKVENVMQALTMTNHNGFPVVDEPPF DSSELCGLVLRSHLLVLLKGKKFTKQRV
Sbjct: 611 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFLDSSELCGLVLRSHLLVLLKGKKFTKQRV 670

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA
Sbjct: 671 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 730

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK
Sbjct: 731 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 781

BLAST of MS012557 vs. NCBI nr
Match: XP_023552785.1 (chloride channel protein CLC-c-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1271.9 bits (3290), Expect = 0.0e+00
Identity = 644/711 (90.58%), Postives = 672/711 (94.51%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSR K+EIFQY+ILKWTLCL IGL TG+VGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFKQDWRSRRKVEIFQYVILKWTLCLFIGLITGVVGFFNNIAVENIAGHKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KYYQAFAVYV SN+VLA AAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLKEKYYQAFAVYVGSNIVLAAAAATLCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAI+LLGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 308 CRGGKCGLFGEGGLIMFEIHTENSAYGTPDLIAIILLGVIGGVLGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVV VSILTTCVSFGLPW SQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKLILVVGVSILTTCVSFGLPWFSQCLPCPSDLDDQCPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIR+L TSA DK FQLSSLFIFF AIYCLGIITYGIAVPSGLF
Sbjct: 428 PGHYNDLASLFFNTNDDAIRSLLTSASDKHFQLSSLFIFFGAIYCLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRI+GRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIIGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL  TNHNGFPV+DE P SDSSELCGLVLRSHLLVLLK KKFTK++V
Sbjct: 608 TFSGIEKVDNIIHALKTTNHNGFPVIDELPSSDSSELCGLVLRSHLLVLLKEKKFTKKKV 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+  FKAHDFAKAGSGKGVKLEDL+ +EEEMEMFVDLHP+TNTSPYTVVE+MSLA
Sbjct: 668 SIRSEILRHFKAHDFAKAGSGKGVKLEDLNFNEEEMEMFVDLHPVTNTSPYTVVESMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEHVLGLYPHLNPHK
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHVLGLYPHLNPHK 776

BLAST of MS012557 vs. NCBI nr
Match: XP_022929418.1 (chloride channel protein CLC-c-like isoform X1 [Cucurbita moschata] >KAG6577357.1 Chloride channel protein CLC-c, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015444.1 Chloride channel protein CLC-c [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 643/711 (90.44%), Postives = 672/711 (94.51%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSR K+EIFQY+ILKWTLCL IGL TG+VGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFKQDWRSRRKVEIFQYVILKWTLCLFIGLITGVVGFFNNIAVENIAGHKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KYYQAFAVYV SN+VLA AAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLKEKYYQAFAVYVGSNIVLAAAAATLCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAI+LLGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 308 CRGGKCGLFGEGGLIMFEIHTENSAYGTPDLIAIILLGVIGGVLGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVV VSILTTCVSFGLPW SQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKLILVVGVSILTTCVSFGLPWFSQCLPCPSDLDDQCPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIR+L TSA DK FQLSSLFIFF AIYCLGIITYGIAVPSGLF
Sbjct: 428 PGHYNDLASLFFNTNDDAIRSLLTSASDKHFQLSSLFIFFGAIYCLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRI+GRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIIGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL  TNHNGFPV+DE P SDSSELCGLVLRSHLLVLLK KKFTK++V
Sbjct: 608 TFSGIEKVDNIIHALKTTNHNGFPVIDELPSSDSSELCGLVLRSHLLVLLKEKKFTKKKV 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+  FKAHDFAKAGSGKGVKLEDL+ +EEEMEMFVDLHP+TNTSPYTVVE+MSLA
Sbjct: 668 SIRSEILRHFKAHDFAKAGSGKGVKLEDLNFNEEEMEMFVDLHPVTNTSPYTVVESMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 776

BLAST of MS012557 vs. NCBI nr
Match: XP_022984849.1 (chloride channel protein CLC-c-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1270.0 bits (3285), Expect = 0.0e+00
Identity = 643/711 (90.44%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSR K+EIFQY+ILKWTLCL IGL TG+VGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFKQDWRSRRKVEIFQYVILKWTLCLFIGLITGVVGFFNNIAVENIAGHKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KYYQAFAVYV SN+VLA AAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLKEKYYQAFAVYVGSNIVLAAAAATLCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAI+LLGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 308 CRGGKCGLFGEGGLIMFEIHTENSAYGTPDLIAIILLGVIGGVLGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVV VSILTTCVSFGLPW SQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKLILVVGVSILTTCVSFGLPWFSQCLPCPSDLDDQCPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIR+L TSA DK FQLSSLFIFF AIYCLGIITYGIAVPSGLF
Sbjct: 428 PGHYNDLASLFFNTNDDAIRSLLTSASDKHFQLSSLFIFFGAIYCLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRI+GRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIIGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL  TNHNGFPV+DE P S+SSELCGLVLRSHLLVLLK KKFTKQ+V
Sbjct: 608 TFSGIEKVDNIIHALKTTNHNGFPVIDELPSSNSSELCGLVLRSHLLVLLKEKKFTKQKV 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+  FKAHDFAKAGSGKGVKLEDL+ +EEEMEMF DLHPITNTSPYTVVE+MSLA
Sbjct: 668 SIRSEILRHFKAHDFAKAGSGKGVKLEDLNFNEEEMEMFADLHPITNTSPYTVVESMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 776

BLAST of MS012557 vs. NCBI nr
Match: XP_008452224.1 (PREDICTED: LOW QUALITY PROTEIN: chloride channel protein CLC-c [Cucumis melo])

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 644/711 (90.58%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIEN+LFKQDWRSR KIEIFQYIILKW LCL IGL TGLVGF NNIAVENIAG KLL+TN
Sbjct: 81  IIENELFKQDWRSRRKIEIFQYIILKWALCLCIGLITGLVGFFNNIAVENIAGHKLLLTN 140

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KY+QAF VYV SN VLAVAAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 141 NLMLKEKYFQAFVVYVGSNSVLAVAAAILCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 200

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 201 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 260

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGA AGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLR FM  
Sbjct: 261 RRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRSFMEF 320

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF I+TENS YGTPDLIAIVLLGVIGGVFGSLYN+LVDKVLRTYS
Sbjct: 321 CRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYS 380

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVVAVSILTTCVSFGLPWLSQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 381 IINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPTVGRSGNYKNFQCP 440

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIRNLFTSA DK FQLSSLF+FF +IYCLGIITYGIAVPSGLF
Sbjct: 441 PGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLGIITYGIAVPSGLF 500

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRIVGRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 501 IPVILAGASYGRIVGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 560

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 561 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 620

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL MTNHNGFPV+DEPPFSDSSELCGLVLRSHLLVLL+ K FTK++V
Sbjct: 621 TFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLREKXFTKKKV 680

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           S+RS I   FKAHDFAK GSGKGVKLEDL+ +EEE+EMFVDLHPITNTSPYTVVE+MSLA
Sbjct: 681 SLRSEIWRGFKAHDFAKPGSGKGVKLEDLEFNEEELEMFVDLHPITNTSPYTVVESMSLA 740

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 741 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 789

BLAST of MS012557 vs. ExPASy Swiss-Prot
Match: Q96282 (Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 549/712 (77.11%), Postives = 620/712 (87.08%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I END FKQDWRSR KIEI QY  LKW L  LIGL TGLVGF NN+ VENIAG KLL+  
Sbjct: 70  IFENDFFKQDWRSRKKIEILQYTFLKWALAFLIGLATGLVGFLNNLGVENIAGFKLLLIG 129

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KY+QAF  +   N++LA AAA+LCA+IAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 130 NLMLKEKYFQAFFAFAGCNLILATAAASLCAFIAPAAAGSGIPEVKAYLNGIDAYSILAP 189

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIF VA GFVVGKEGPMVHTGACIA+LLGQGGS+KYRLTWKWLR+FKNDRD
Sbjct: 190 STLFVKIFGSIFGVAAGFVVGKEGPMVHTGACIANLLGQGGSKKYRLTWKWLRFFKNDRD 249

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGA AGVAAAFRAPVGGVLFALEEAASWWR++LLWRTFFTTAVVAVVLR  +  
Sbjct: 250 RRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRNALLWRTFFTTAVVAVVLRSLIEF 309

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CR G CGLFG+GGLIMFD+++    Y TPDL+AIV LGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 310 CRSGRCGLFGKGGLIMFDVNSGPVLYSTPDLLAIVFLGVIGGVLGSLYNYLVDKVLRTYS 369

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDD-QCPTVGRSGNYKNFQC 360
           IINE+GP +K++LV+AVSIL++C +FGLPWLSQC PCP  +++ +CP+VGRS  YK+FQC
Sbjct: 370 IINEKGPRFKIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQC 429

Query: 361 PPGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGL 420
           PP HYNDL+SL   TNDDAIRNLFTS  + +F +S+L IFF A+YCLGIITYGIA+PSGL
Sbjct: 430 PPNHYNDLSSLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGL 489

Query: 421 FIPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTN 480
           FIPVILAGASYGR+VGRL G V  + LDV L++LLGAASFLGGTMRMTVSLCVILLELTN
Sbjct: 490 FIPVILAGASYGRLVGRLLGPV--SQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTN 549

Query: 481 NLLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPL 540
           NLLMLPL+MLVLLISKTVAD FN+GVYDQIV MKGLP+ME HAEPYMR L+A  V SG L
Sbjct: 550 NLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSGAL 609

Query: 541 ITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQR 600
           I+ S +EKV  + QAL MT HNGFPV+DEPPF+++SELCG+ LRSHLLVLL+GKKF+KQR
Sbjct: 610 ISFSRVEKVGVIWQALKMTRHNGFPVIDEPPFTEASELCGIALRSHLLVLLQGKKFSKQR 669

Query: 601 VSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSL 660
            +  S I+ S KA DF KAG GKG+K+EDLD+ EEEMEM+VDLHPITNTSPYTV+ET+SL
Sbjct: 670 TTFGSQILRSCKARDFGKAGLGKGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSL 729

Query: 661 AKAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           AKAAILF  LGLRHL VVPKTPGRPPI GILTRHDFMPEHVLGLYPH++P K
Sbjct: 730 AKAAILFRQLGLRHLCVVPKTPGRPPIVGILTRHDFMPEHVLGLYPHIDPLK 779

BLAST of MS012557 vs. ExPASy Swiss-Prot
Match: P60300 (Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=CLC-G PE=1 SV=2)

HSP 1 Score: 918.7 bits (2373), Expect = 4.1e-266
Identity = 434/711 (61.04%), Postives = 567/711 (79.75%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I END FKQDWR R+K+EIFQY+ +KW LC  IG+   L+GF+NN+AVEN+AGVK ++T+
Sbjct: 45  IAENDFFKQDWRGRSKVEIFQYVFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTS 104

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           ++M+  ++   F V+  +N++L + A+ + A++APAAAGSGIPEVKAYLNG+DA  I + 
Sbjct: 105 NMMIAGRFAMGFVVFSVTNLILTLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSL 164

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
            TL +KI G+I AV+   ++GK GPMVHTGAC+AS+LGQGGS++YRLTW+WLR+FKNDRD
Sbjct: 165 RTLIIKIIGNISAVSASLLIGKAGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRD 224

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDL+TCGA AG+AA+FRAPVGGVLFALEE +SWWRS+LLWR FF+TAVVA+VLR  + +
Sbjct: 225 RRDLVTCGAAAGIAASFRAPVGGVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDV 284

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG+GGLIMFD+++EN++Y   D++ ++LLGV+GG+ GSLYNFL+DKVLR Y+
Sbjct: 285 CLSGKCGLFGKGGLIMFDVYSENASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYN 344

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
            I E+G  WK++L  A+SI T+C+ FGLP+L+ C PCP D  ++CPT+GRSGN+K +QCP
Sbjct: 345 YIYEKGVTWKILLACAISIFTSCLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCP 404

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASL F TNDDAI+NLF+   D +F   S+ +FF   + L I +YGI  P+GLF
Sbjct: 405 PGHYNDLASLIFNTNDDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLF 464

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +PVI+ GASYGR VG L GS  +++L+  L+A+LGAASFLGGTMRMTVS CVILLELTNN
Sbjct: 465 VPVIVTGASYGRFVGMLLGS--NSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNN 524

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LL+LP++M+VLLISKTVAD FN  +Y+ I+K+KG P++ +HAEPYMRQL+ G V +GPL 
Sbjct: 525 LLLLPMMMVVLLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQ 584

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
             +GIEKVE ++  L  TNHNGFPVVD PP + +  L GL+LR+H+L LLK + F    V
Sbjct: 585 VFNGIEKVETIVHVLKTTNHNGFPVVDGPPLAAAPVLHGLILRAHILTLLKKRVFMPSPV 644

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           +  S  +  FKA +FAK GSG+  K+ED+++ EEE+ M++DLHP +N SPYTVVETMSLA
Sbjct: 645 ACDSNTLSQFKAEEFAKKGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLA 704

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KA ILF  +G+RHLLV+PKT  RPP+ GILTRHDFMPEH+LGL+P ++  K
Sbjct: 705 KALILFREVGIRHLLVIPKTSNRPPVVGILTRHDFMPEHILGLHPSVSRSK 753

BLAST of MS012557 vs. ExPASy Swiss-Prot
Match: P92941 (Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=2)

HSP 1 Score: 771.9 bits (1992), Expect = 6.1e-222
Identity = 393/716 (54.89%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I ENDLFK DWRSR+K ++FQYI LKWTL  L+GLFTGL+    N+AVENIAG KLL   
Sbjct: 61  INENDLFKHDWRSRSKAQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVG 120

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
             + + +++    V+  +N+ L + A  L  Y AP AAG GIPE+KAYLNGID  ++   
Sbjct: 121 YYIAQDRFWTGLMVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGF 180

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           +T+ VKI GSI AVA G  +GKEGP+VH G+CIASLLGQGG   +R+ W+WLRYF NDRD
Sbjct: 181 TTMMVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRD 240

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCG+ +GV AAFR+PVGGVLFALEE A+WWRS+LLWRTFF+TAVV VVLR F+ I
Sbjct: 241 RRDLITCGSASGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEI 300

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG GGLIMFD+      Y   D+I + L+GV GG+ GSLYN L+ KVLR Y+
Sbjct: 301 CNSGKCGLFGSGGLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYN 360

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           +IN++G   KV+L + VS+ T+   FGLP+L++C PC P +D+ CPT GRSGN+K F CP
Sbjct: 361 LINQKGKIHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCP 420

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
            G+YNDL++L  TTNDDA+RN+F+S    +F + SL+IFF     LG+IT+GIA PSGLF
Sbjct: 421 NGYYNDLSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLF 480

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +P+IL G++YGR++G   GS    ++D  LYA+LGAAS + G+MRMTVSLCVI LELTNN
Sbjct: 481 LPIILMGSAYGRMLGTAMGSY--TNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNN 540

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGV--ASGP 540
           LL+LP+ M VLLI+KTV D FN  +Y+ I+ +KGLPF+EA+ EP+MR L  G +  A  P
Sbjct: 541 LLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPP 600

Query: 541 LITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKF-TK 600
           ++TL+G+EKV N++  L  T HN FPV+D    +  +EL GL+LR+HL+ +LK + F  +
Sbjct: 601 VVTLNGVEKVANIVDVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNE 660

Query: 601 QRVSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETM 660
           +R +    +   F   + A+    +    +D+ I   EM+++VDLHP+TNT+PYTVV++M
Sbjct: 661 KRRTEEWEVREKFTPVELAE----REDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSM 720

Query: 661 SLAKAAILFHGLGLRHLLVVPK--TPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           S+AKA +LF  +GLRHLLVVPK    G  P+ GILTR D    ++L  +PHL+ HK
Sbjct: 721 SVAKALVLFRSVGLRHLLVVPKIQASGMSPVIGILTRQDLRAYNILQAFPHLDKHK 770

BLAST of MS012557 vs. ExPASy Swiss-Prot
Match: P92942 (Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 1.0e-221
Identity = 397/722 (54.99%), Postives = 517/722 (71.61%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I ENDLFK DWR R+K ++ QY+ LKWTL  L+GLFTGL+    N+AVENIAG KLL   
Sbjct: 60  INENDLFKHDWRKRSKAQVLQYVFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVG 119

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
             + +++Y     V V +N+ L + A+ LC   AP AAG GIPE+KAYLNG+D  ++   
Sbjct: 120 HFLTQERYVTGLMVLVGANLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGA 179

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           +T+ VKI GSI AVA G  +GKEGP+VH G+CIASLLGQGG+  +R+ W+WLRYF NDRD
Sbjct: 180 TTMIVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRD 239

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCG+ AGV AAFR+PVGGVLFALEE A+WWRS+LLWRTFF+TAVV VVLR F+ I
Sbjct: 240 RRDLITCGSAAGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEI 299

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG+GGLIMFD+      Y   D+I ++L+GVIGG+ GSLYN L+ KVLR Y+
Sbjct: 300 CNSGKCGLFGKGGLIMFDVSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYN 359

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           +INE+G   KV+L + VS+ T+   +GLP+L++C PC P +D+ CPT GRSGN+K F CP
Sbjct: 360 LINEKGKIHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCP 419

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
            G+YNDLA+L  TTNDDA+RNLF+S    +F + SL+IFF     LG+ T+GIA PSGLF
Sbjct: 420 KGYYNDLATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLF 479

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +P+IL GA+YGR++G   GS     +D  LYA+LGAA+ + G+MRMTVSLCVI LELTNN
Sbjct: 480 LPIILMGAAYGRMLGAAMGSY--TSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNN 539

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAG--GVASGP 540
           LL+LP+ M+VLLI+KTV D FN  +YD I+ +KGLPF+EA+ EP+MR L  G  G A  P
Sbjct: 540 LLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPP 599

Query: 541 LITLSGIEKVENVMQALTMTNHNGFPVVDEPPF------SDSSELCGLVLRSHLLVLLKG 600
           ++TL G+EKV N++  L  T HN FPV+DE         + ++EL GL+LR+HL+ +LK 
Sbjct: 600 VVTLQGVEKVSNIVDVLKNTTHNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKK 659

Query: 601 KKF-TKQRVSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPY 660
           + F T++R +    +   F   + A+    +    +D+ I   EMEM+VDLHP+TNT+PY
Sbjct: 660 RWFLTEKRRTEEWEVREKFPWDELAE----REDNFDDVAITSAEMEMYVDLHPLTNTTPY 719

Query: 661 TVVETMSLAKAAILFHGLGLRHLLVVPK--TPGRPPITGILTRHDFMPEHVLGLYPHLNP 712
           TV+E MS+AKA +LF  +GLRHLL+VPK    G  P+ GILTR D    ++L  +P L  
Sbjct: 720 TVMENMSVAKALVLFRQVGLRHLLIVPKIQASGMCPVVGILTRQDLRAYNILQAFPLLEK 775

BLAST of MS012557 vs. ExPASy Swiss-Prot
Match: P92943 (Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=2)

HSP 1 Score: 609.8 bits (1571), Expect = 4.0e-173
Identity = 346/710 (48.73%), Postives = 469/710 (66.06%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           +IEN  ++++   R K+ +  Y+ +KW   LLIG+ TGL     N++VEN AG K  +T 
Sbjct: 51  VIENYAYREEQAHRGKLYVGYYVAVKWFFSLLIGIGTGLAAVFINLSVENFAGWKFALTF 110

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           ++ +++ Y+  F VY+  N+VL  ++A +    APAAAGSGIPE+K YLNGID    L  
Sbjct: 111 AI-IQKSYFAGFIVYLLINLVLVFSSAYIITQFAPAAAGSGIPEIKGYLNGIDIPGTLLF 170

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
            TL  KIFGSI +V GG  +GKEGP+VHTGACIASLLGQGGS KY L  +W + FK+DRD
Sbjct: 171 RTLIGKIFGSIGSVGGGLALGKEGPLVHTGACIASLLGQGGSTKYHLNSRWPQLFKSDRD 230

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDL+TCG  AGVAAAFRAPVGGVLFALEE  SWWRS L+WR FFT+A+VAVV+R  M  
Sbjct: 231 RRDLVTCGCAAGVAAAFRAPVGGVLFALEEVTSWWRSQLMWRVFFTSAIVAVVVRTAMGW 290

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLR-TY 300
           C+ G CG FG GG I++D+     +Y   +L+ + ++GVIGG+ G+L+N L   +     
Sbjct: 291 CKSGICGHFGGGGFIIWDVSDGQDDYYFKELLPMAVIGVIGGLLGALFNQLTLYMTSWRR 350

Query: 301 SIINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCP---PDLDDQCP-TVGRSGNYK 360
           + ++++G   K+I    +S +T+ +SFGLP L +C PCP   PD   +CP   G  GNY 
Sbjct: 351 NSLHKKGNRVKIIEACIISCITSAISFGLPLLRKCSPCPESVPDSGIECPRPPGMYGNYV 410

Query: 361 NFQC-PPGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIA 420
           NF C     YNDLA++FF T DDAIRNLF++   ++F   SL  F A  Y L ++T+G A
Sbjct: 411 NFFCKTDNEYNDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLAVVTFGTA 470

Query: 421 VPSGLFIPVILAGASYGRIVGR-LFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVI 480
           VP+G F+P I+ G++YGR+VG  +       +++   YALLGAASFLGG+MRMTVSLCVI
Sbjct: 471 VPAGQFVPGIMIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRMTVSLCVI 530

Query: 481 LLELTNNLLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIA-G 540
           ++E+TNNL +LPL+MLVLLISK V D FN+G+Y+   ++KG+P +E+  + +MRQ+IA  
Sbjct: 531 MVEITNNLKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKE 590

Query: 541 GVASGPLITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKG 600
              S  +I+L  + +V +V   L    HNGFPV+D    S  + + GLVLRSHLLVLL+ 
Sbjct: 591 ACQSQKVISLPRVIRVADVASILGSNKHNGFPVIDHTR-SGETLVIGLVLRSHLLVLLQS 650

Query: 601 K-KFTKQRVSI---RSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNT 660
           K  F    +        I  SF   +FAK  S KG+ +ED+ +  +++EM++DL P  N 
Sbjct: 651 KVDFQHSPLPCDPSARNIRHSFS--EFAKPVSSKGLCIEDIHLTSDDLEMYIDLAPFLNP 710

Query: 661 SPYTVVETMSLAKAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPE 699
           SPY V E MSL K   LF  LGLRHL VVP+ P R  + G++TR D + E
Sbjct: 711 SPYVVPEDMSLTKVYNLFRQLGLRHLFVVPR-PSR--VIGLITRKDLLIE 753

BLAST of MS012557 vs. ExPASy TrEMBL
Match: A0A6J1C5E7 (chloride channel protein CLC-c OS=Momordica charantia OX=3673 GN=LOC111008418 PE=3 SV=1)

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN
Sbjct: 71  IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 130

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 131 SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 190

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 191 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 250

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI
Sbjct: 251 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 310

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS
Sbjct: 311 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 370

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP
Sbjct: 371 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 430

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF
Sbjct: 431 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 490

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 491 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 550

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI
Sbjct: 551 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 610

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           TLSGIEKVENVMQALTMTNHNGFPVVDEPPF DSSELCGLVLRSHLLVLLKGKKFTKQRV
Sbjct: 611 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFLDSSELCGLVLRSHLLVLLKGKKFTKQRV 670

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA
Sbjct: 671 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 730

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK
Sbjct: 731 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 781

BLAST of MS012557 vs. ExPASy TrEMBL
Match: A0A6J1ES18 (Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111435995 PE=3 SV=1)

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 643/711 (90.44%), Postives = 672/711 (94.51%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSR K+EIFQY+ILKWTLCL IGL TG+VGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFKQDWRSRRKVEIFQYVILKWTLCLFIGLITGVVGFFNNIAVENIAGHKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KYYQAFAVYV SN+VLA AAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLKEKYYQAFAVYVGSNIVLAAAAATLCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAI+LLGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 308 CRGGKCGLFGEGGLIMFEIHTENSAYGTPDLIAIILLGVIGGVLGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVV VSILTTCVSFGLPW SQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKLILVVGVSILTTCVSFGLPWFSQCLPCPSDLDDQCPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIR+L TSA DK FQLSSLFIFF AIYCLGIITYGIAVPSGLF
Sbjct: 428 PGHYNDLASLFFNTNDDAIRSLLTSASDKHFQLSSLFIFFGAIYCLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRI+GRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIIGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL  TNHNGFPV+DE P SDSSELCGLVLRSHLLVLLK KKFTK++V
Sbjct: 608 TFSGIEKVDNIIHALKTTNHNGFPVIDELPSSDSSELCGLVLRSHLLVLLKEKKFTKKKV 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+  FKAHDFAKAGSGKGVKLEDL+ +EEEMEMFVDLHP+TNTSPYTVVE+MSLA
Sbjct: 668 SIRSEILRHFKAHDFAKAGSGKGVKLEDLNFNEEEMEMFVDLHPVTNTSPYTVVESMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 776

BLAST of MS012557 vs. ExPASy TrEMBL
Match: A0A6J1JBP4 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111483006 PE=3 SV=1)

HSP 1 Score: 1270.0 bits (3285), Expect = 0.0e+00
Identity = 643/711 (90.44%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLFKQDWRSR K+EIFQY+ILKWTLCL IGL TG+VGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFKQDWRSRRKVEIFQYVILKWTLCLFIGLITGVVGFFNNIAVENIAGHKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KYYQAFAVYV SN+VLA AAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLKEKYYQAFAVYVGSNIVLAAAAATLCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAI+LLGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 308 CRGGKCGLFGEGGLIMFEIHTENSAYGTPDLIAIILLGVIGGVLGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVV VSILTTCVSFGLPW SQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKLILVVGVSILTTCVSFGLPWFSQCLPCPSDLDDQCPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIR+L TSA DK FQLSSLFIFF AIYCLGIITYGIAVPSGLF
Sbjct: 428 PGHYNDLASLFFNTNDDAIRSLLTSASDKHFQLSSLFIFFGAIYCLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRI+GRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIIGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL  TNHNGFPV+DE P S+SSELCGLVLRSHLLVLLK KKFTKQ+V
Sbjct: 608 TFSGIEKVDNIIHALKTTNHNGFPVIDELPSSNSSELCGLVLRSHLLVLLKEKKFTKQKV 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+  FKAHDFAKAGSGKGVKLEDL+ +EEEMEMF DLHPITNTSPYTVVE+MSLA
Sbjct: 668 SIRSEILRHFKAHDFAKAGSGKGVKLEDLNFNEEEMEMFADLHPITNTSPYTVVESMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 776

BLAST of MS012557 vs. ExPASy TrEMBL
Match: A0A1S3BUH5 (LOW QUALITY PROTEIN: chloride channel protein CLC-c OS=Cucumis melo OX=3656 GN=LOC103493306 PE=3 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 644/711 (90.58%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIEN+LFKQDWRSR KIEIFQYIILKW LCL IGL TGLVGF NNIAVENIAG KLL+TN
Sbjct: 81  IIENELFKQDWRSRRKIEIFQYIILKWALCLCIGLITGLVGFFNNIAVENIAGHKLLLTN 140

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KY+QAF VYV SN VLAVAAA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 141 NLMLKEKYFQAFVVYVGSNSVLAVAAAILCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 200

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 201 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 260

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGA AGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLR FM  
Sbjct: 261 RRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRSFMEF 320

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF I+TENS YGTPDLIAIVLLGVIGGVFGSLYN+LVDKVLRTYS
Sbjct: 321 CRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYS 380

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG K+ILVVAVSILTTCVSFGLPWLSQCLPCP DLDDQCPTVGRSGNYKNFQCP
Sbjct: 381 IINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPTVGRSGNYKNFQCP 440

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASLFF TNDDAIRNLFTSA DK FQLSSLF+FF +IYCLGIITYGIAVPSGLF
Sbjct: 441 PGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLGIITYGIAVPSGLF 500

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRIVGRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 501 IPVILAGASYGRIVGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 560

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 561 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 620

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL MTNHNGFPV+DEPPFSDSSELCGLVLRSHLLVLL+ K FTK++V
Sbjct: 621 TFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLREKXFTKKKV 680

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           S+RS I   FKAHDFAK GSGKGVKLEDL+ +EEE+EMFVDLHPITNTSPYTVVE+MSLA
Sbjct: 681 SLRSEIWRGFKAHDFAKPGSGKGVKLEDLEFNEEELEMFVDLHPITNTSPYTVVESMSLA 740

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPEH+LGLYPHLNPHK
Sbjct: 741 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPHK 789

BLAST of MS012557 vs. ExPASy TrEMBL
Match: A0A6J1FPV1 (chloride channel protein CLC-c-like OS=Cucurbita moschata OX=3662 GN=LOC111447712 PE=3 SV=1)

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 642/710 (90.42%), Postives = 674/710 (94.93%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           IIENDLF QDWRSR KIEIFQY+ILKW LCLLIGL TGLVGF NNIAVENIAG KLL+TN
Sbjct: 68  IIENDLFMQDWRSRRKIEIFQYVILKWALCLLIGLVTGLVGFFNNIAVENIAGRKLLLTN 127

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LML++KYY AFAVYV SN+VLA +AAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 128 NLMLQEKYYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 187

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD
Sbjct: 188 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 247

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGA AGVAAAFRAPVGGVLFALEEAASWWRS+LLWRTFFTTAVVAVVLRGFM  
Sbjct: 248 RRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEF 307

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CRGG CGLFGEGGLIMF+IHTENS YGTPDLIAIVLLGVIGGVFGSLYN+LVDKVLRTYS
Sbjct: 308 CRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYS 367

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           IINERGPG KVILVV++S+LTTC+SFGLPWLSQCLPCPP L+D+CPTVGRSGNYKNFQCP
Sbjct: 368 IINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPHLEDECPTVGRSGNYKNFQCP 427

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PG YNDLASLFF TNDD IRNLFTSA DKQFQLSSLFIFF +IY LGIITYGIAVPSGLF
Sbjct: 428 PGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLF 487

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           IPVILAGASYGRIVGRLFGSV  A LDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN
Sbjct: 488 IPVILAGASYGRIVGRLFGSV--ATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 547

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LLMLPLLMLVLLISK+VAD FNKGVYDQIVKMKGLPFMEAHAEP+MRQL+AGGVASGPLI
Sbjct: 548 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLI 607

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
           T SGIEKV+N++ AL MTNHNGFPV+DEPPFSDSSELCGLVLRSHLLVLLK KKFTK++ 
Sbjct: 608 TFSGIEKVDNIIHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKT 667

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           SIRS I+ SFKA+DFAK GSGKGVKLEDLDI+EEEMEMFVDLHPITNTSPYTVVETMSLA
Sbjct: 668 SIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLA 727

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPH 711
           KAAILFH LGLRHLLVVPKTPGRPPI GILTRHDFMPE++LGLYP+LNPH
Sbjct: 728 KAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 775

BLAST of MS012557 vs. TAIR 10
Match: AT5G49890.1 (chloride channel C )

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 549/712 (77.11%), Postives = 620/712 (87.08%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I END FKQDWRSR KIEI QY  LKW L  LIGL TGLVGF NN+ VENIAG KLL+  
Sbjct: 70  IFENDFFKQDWRSRKKIEILQYTFLKWALAFLIGLATGLVGFLNNLGVENIAGFKLLLIG 129

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           +LMLK+KY+QAF  +   N++LA AAA+LCA+IAPAAAGSGIPEVKAYLNGIDAYSILAP
Sbjct: 130 NLMLKEKYFQAFFAFAGCNLILATAAASLCAFIAPAAAGSGIPEVKAYLNGIDAYSILAP 189

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           STLFVKIFGSIF VA GFVVGKEGPMVHTGACIA+LLGQGGS+KYRLTWKWLR+FKNDRD
Sbjct: 190 STLFVKIFGSIFGVAAGFVVGKEGPMVHTGACIANLLGQGGSKKYRLTWKWLRFFKNDRD 249

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCGA AGVAAAFRAPVGGVLFALEEAASWWR++LLWRTFFTTAVVAVVLR  +  
Sbjct: 250 RRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRNALLWRTFFTTAVVAVVLRSLIEF 309

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           CR G CGLFG+GGLIMFD+++    Y TPDL+AIV LGVIGGV GSLYN+LVDKVLRTYS
Sbjct: 310 CRSGRCGLFGKGGLIMFDVNSGPVLYSTPDLLAIVFLGVIGGVLGSLYNYLVDKVLRTYS 369

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDD-QCPTVGRSGNYKNFQC 360
           IINE+GP +K++LV+AVSIL++C +FGLPWLSQC PCP  +++ +CP+VGRS  YK+FQC
Sbjct: 370 IINEKGPRFKIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQC 429

Query: 361 PPGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGL 420
           PP HYNDL+SL   TNDDAIRNLFTS  + +F +S+L IFF A+YCLGIITYGIA+PSGL
Sbjct: 430 PPNHYNDLSSLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGL 489

Query: 421 FIPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTN 480
           FIPVILAGASYGR+VGRL G V  + LDV L++LLGAASFLGGTMRMTVSLCVILLELTN
Sbjct: 490 FIPVILAGASYGRLVGRLLGPV--SQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTN 549

Query: 481 NLLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPL 540
           NLLMLPL+MLVLLISKTVAD FN+GVYDQIV MKGLP+ME HAEPYMR L+A  V SG L
Sbjct: 550 NLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSGAL 609

Query: 541 ITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQR 600
           I+ S +EKV  + QAL MT HNGFPV+DEPPF+++SELCG+ LRSHLLVLL+GKKF+KQR
Sbjct: 610 ISFSRVEKVGVIWQALKMTRHNGFPVIDEPPFTEASELCGIALRSHLLVLLQGKKFSKQR 669

Query: 601 VSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSL 660
            +  S I+ S KA DF KAG GKG+K+EDLD+ EEEMEM+VDLHPITNTSPYTV+ET+SL
Sbjct: 670 TTFGSQILRSCKARDFGKAGLGKGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSL 729

Query: 661 AKAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           AKAAILF  LGLRHL VVPKTPGRPPI GILTRHDFMPEHVLGLYPH++P K
Sbjct: 730 AKAAILFRQLGLRHLCVVPKTPGRPPIVGILTRHDFMPEHVLGLYPHIDPLK 779

BLAST of MS012557 vs. TAIR 10
Match: AT5G33280.1 (Voltage-gated chloride channel family protein )

HSP 1 Score: 918.7 bits (2373), Expect = 2.9e-267
Identity = 434/711 (61.04%), Postives = 567/711 (79.75%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I END FKQDWR R+K+EIFQY+ +KW LC  IG+   L+GF+NN+AVEN+AGVK ++T+
Sbjct: 45  IAENDFFKQDWRGRSKVEIFQYVFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTS 104

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
           ++M+  ++   F V+  +N++L + A+ + A++APAAAGSGIPEVKAYLNG+DA  I + 
Sbjct: 105 NMMIAGRFAMGFVVFSVTNLILTLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSL 164

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
            TL +KI G+I AV+   ++GK GPMVHTGAC+AS+LGQGGS++YRLTW+WLR+FKNDRD
Sbjct: 165 RTLIIKIIGNISAVSASLLIGKAGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRD 224

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDL+TCGA AG+AA+FRAPVGGVLFALEE +SWWRS+LLWR FF+TAVVA+VLR  + +
Sbjct: 225 RRDLVTCGAAAGIAASFRAPVGGVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDV 284

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG+GGLIMFD+++EN++Y   D++ ++LLGV+GG+ GSLYNFL+DKVLR Y+
Sbjct: 285 CLSGKCGLFGKGGLIMFDVYSENASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYN 344

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
            I E+G  WK++L  A+SI T+C+ FGLP+L+ C PCP D  ++CPT+GRSGN+K +QCP
Sbjct: 345 YIYEKGVTWKILLACAISIFTSCLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCP 404

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
           PGHYNDLASL F TNDDAI+NLF+   D +F   S+ +FF   + L I +YGI  P+GLF
Sbjct: 405 PGHYNDLASLIFNTNDDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLF 464

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +PVI+ GASYGR VG L GS  +++L+  L+A+LGAASFLGGTMRMTVS CVILLELTNN
Sbjct: 465 VPVIVTGASYGRFVGMLLGS--NSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNN 524

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGVASGPLI 540
           LL+LP++M+VLLISKTVAD FN  +Y+ I+K+KG P++ +HAEPYMRQL+ G V +GPL 
Sbjct: 525 LLLLPMMMVVLLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQ 584

Query: 541 TLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKFTKQRV 600
             +GIEKVE ++  L  TNHNGFPVVD PP + +  L GL+LR+H+L LLK + F    V
Sbjct: 585 VFNGIEKVETIVHVLKTTNHNGFPVVDGPPLAAAPVLHGLILRAHILTLLKKRVFMPSPV 644

Query: 601 SIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLA 660
           +  S  +  FKA +FAK GSG+  K+ED+++ EEE+ M++DLHP +N SPYTVVETMSLA
Sbjct: 645 ACDSNTLSQFKAEEFAKKGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLA 704

Query: 661 KAAILFHGLGLRHLLVVPKTPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           KA ILF  +G+RHLLV+PKT  RPP+ GILTRHDFMPEH+LGL+P ++  K
Sbjct: 705 KALILFREVGIRHLLVIPKTSNRPPVVGILTRHDFMPEHILGLHPSVSRSK 753

BLAST of MS012557 vs. TAIR 10
Match: AT5G40890.1 (chloride channel A )

HSP 1 Score: 771.9 bits (1992), Expect = 4.4e-223
Identity = 393/716 (54.89%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I ENDLFK DWRSR+K ++FQYI LKWTL  L+GLFTGL+    N+AVENIAG KLL   
Sbjct: 61  INENDLFKHDWRSRSKAQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVG 120

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
             + + +++    V+  +N+ L + A  L  Y AP AAG GIPE+KAYLNGID  ++   
Sbjct: 121 YYIAQDRFWTGLMVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGF 180

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           +T+ VKI GSI AVA G  +GKEGP+VH G+CIASLLGQGG   +R+ W+WLRYF NDRD
Sbjct: 181 TTMMVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRD 240

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCG+ +GV AAFR+PVGGVLFALEE A+WWRS+LLWRTFF+TAVV VVLR F+ I
Sbjct: 241 RRDLITCGSASGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEI 300

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG GGLIMFD+      Y   D+I + L+GV GG+ GSLYN L+ KVLR Y+
Sbjct: 301 CNSGKCGLFGSGGLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYN 360

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           +IN++G   KV+L + VS+ T+   FGLP+L++C PC P +D+ CPT GRSGN+K F CP
Sbjct: 361 LINQKGKIHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCP 420

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
            G+YNDL++L  TTNDDA+RN+F+S    +F + SL+IFF     LG+IT+GIA PSGLF
Sbjct: 421 NGYYNDLSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLF 480

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +P+IL G++YGR++G   GS    ++D  LYA+LGAAS + G+MRMTVSLCVI LELTNN
Sbjct: 481 LPIILMGSAYGRMLGTAMGSY--TNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNN 540

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGV--ASGP 540
           LL+LP+ M VLLI+KTV D FN  +Y+ I+ +KGLPF+EA+ EP+MR L  G +  A  P
Sbjct: 541 LLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPP 600

Query: 541 LITLSGIEKVENVMQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKF-TK 600
           ++TL+G+EKV N++  L  T HN FPV+D    +  +EL GL+LR+HL+ +LK + F  +
Sbjct: 601 VVTLNGVEKVANIVDVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNE 660

Query: 601 QRVSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETM 660
           +R +    +   F   + A+    +    +D+ I   EM+++VDLHP+TNT+PYTVV++M
Sbjct: 661 KRRTEEWEVREKFTPVELAE----REDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSM 720

Query: 661 SLAKAAILFHGLGLRHLLVVPK--TPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           S+AKA +LF  +GLRHLLVVPK    G  P+ GILTR D    ++L  +PHL+ HK
Sbjct: 721 SVAKALVLFRSVGLRHLLVVPKIQASGMSPVIGILTRQDLRAYNILQAFPHLDKHK 770

BLAST of MS012557 vs. TAIR 10
Match: AT3G27170.1 (chloride channel B )

HSP 1 Score: 771.2 bits (1990), Expect = 7.4e-223
Identity = 397/722 (54.99%), Postives = 517/722 (71.61%), Query Frame = 0

Query: 1   IIENDLFKQDWRSRTKIEIFQYIILKWTLCLLIGLFTGLVGFSNNIAVENIAGVKLLMTN 60
           I ENDLFK DWR R+K ++ QY+ LKWTL  L+GLFTGL+    N+AVENIAG KLL   
Sbjct: 60  INENDLFKHDWRKRSKAQVLQYVFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVG 119

Query: 61  SLMLKQKYYQAFAVYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAP 120
             + +++Y     V V +N+ L + A+ LC   AP AAG GIPE+KAYLNG+D  ++   
Sbjct: 120 HFLTQERYVTGLMVLVGANLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGA 179

Query: 121 STLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRD 180
           +T+ VKI GSI AVA G  +GKEGP+VH G+CIASLLGQGG+  +R+ W+WLRYF NDRD
Sbjct: 180 TTMIVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRD 239

Query: 181 RRDLITCGAGAGVAAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAI 240
           RRDLITCG+ AGV AAFR+PVGGVLFALEE A+WWRS+LLWRTFF+TAVV VVLR F+ I
Sbjct: 240 RRDLITCGSAAGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEI 299

Query: 241 CRGGTCGLFGEGGLIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYS 300
           C  G CGLFG+GGLIMFD+      Y   D+I ++L+GVIGG+ GSLYN L+ KVLR Y+
Sbjct: 300 CNSGKCGLFGKGGLIMFDVSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYN 359

Query: 301 IINERGPGWKVILVVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCP 360
           +INE+G   KV+L + VS+ T+   +GLP+L++C PC P +D+ CPT GRSGN+K F CP
Sbjct: 360 LINEKGKIHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCP 419

Query: 361 PGHYNDLASLFFTTNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLF 420
            G+YNDLA+L  TTNDDA+RNLF+S    +F + SL+IFF     LG+ T+GIA PSGLF
Sbjct: 420 KGYYNDLATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLF 479

Query: 421 IPVILAGASYGRIVGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 480
           +P+IL GA+YGR++G   GS     +D  LYA+LGAA+ + G+MRMTVSLCVI LELTNN
Sbjct: 480 LPIILMGAAYGRMLGAAMGSY--TSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNN 539

Query: 481 LLMLPLLMLVLLISKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAG--GVASGP 540
           LL+LP+ M+VLLI+KTV D FN  +YD I+ +KGLPF+EA+ EP+MR L  G  G A  P
Sbjct: 540 LLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPP 599

Query: 541 LITLSGIEKVENVMQALTMTNHNGFPVVDEPPF------SDSSELCGLVLRSHLLVLLKG 600
           ++TL G+EKV N++  L  T HN FPV+DE         + ++EL GL+LR+HL+ +LK 
Sbjct: 600 VVTLQGVEKVSNIVDVLKNTTHNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKK 659

Query: 601 KKF-TKQRVSIRSGIMGSFKAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPY 660
           + F T++R +    +   F   + A+    +    +D+ I   EMEM+VDLHP+TNT+PY
Sbjct: 660 RWFLTEKRRTEEWEVREKFPWDELAE----REDNFDDVAITSAEMEMYVDLHPLTNTTPY 719

Query: 661 TVVETMSLAKAAILFHGLGLRHLLVVPK--TPGRPPITGILTRHDFMPEHVLGLYPHLNP 712
           TV+E MS+AKA +LF  +GLRHLL+VPK    G  P+ GILTR D    ++L  +P L  
Sbjct: 720 TVMENMSVAKALVLFRQVGLRHLLIVPKIQASGMCPVVGILTRQDLRAYNILQAFPLLEK 775

BLAST of MS012557 vs. TAIR 10
Match: AT5G40890.2 (chloride channel A )

HSP 1 Score: 692.2 bits (1785), Expect = 4.4e-199
Identity = 353/643 (54.90%), Postives = 466/643 (72.47%), Query Frame = 0

Query: 74  VYVASNVVLAVAAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFA 133
           V+  +N+ L + A  L  Y AP AAG GIPE+KAYLNGID  ++   +T+ VKI GSI A
Sbjct: 2   VFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIGA 61

Query: 134 VAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAGAGV 193
           VA G  +GKEGP+VH G+CIASLLGQGG   +R+ W+WLRYF NDRDRRDLITCG+ +GV
Sbjct: 62  VAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASGV 121

Query: 194 AAAFRAPVGGVLFALEEAASWWRSSLLWRTFFTTAVVAVVLRGFMAICRGGTCGLFGEGG 253
            AAFR+PVGGVLFALEE A+WWRS+LLWRTFF+TAVV VVLR F+ IC  G CGLFG GG
Sbjct: 122 CAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSGG 181

Query: 254 LIMFDIHTENSNYGTPDLIAIVLLGVIGGVFGSLYNFLVDKVLRTYSIINERGPGWKVIL 313
           LIMFD+      Y   D+I + L+GV GG+ GSLYN L+ KVLR Y++IN++G   KV+L
Sbjct: 182 LIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVLL 241

Query: 314 VVAVSILTTCVSFGLPWLSQCLPCPPDLDDQCPTVGRSGNYKNFQCPPGHYNDLASLFFT 373
            + VS+ T+   FGLP+L++C PC P +D+ CPT GRSGN+K F CP G+YNDL++L  T
Sbjct: 242 SLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLLT 301

Query: 374 TNDDAIRNLFTSAIDKQFQLSSLFIFFAAIYCLGIITYGIAVPSGLFIPVILAGASYGRI 433
           TNDDA+RN+F+S    +F + SL+IFF     LG+IT+GIA PSGLF+P+IL G++YGR+
Sbjct: 302 TNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGRM 361

Query: 434 VGRLFGSVADADLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLI 493
           +G   GS    ++D  LYA+LGAAS + G+MRMTVSLCVI LELTNNLL+LP+ M VLLI
Sbjct: 362 LGTAMGSY--TNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLI 421

Query: 494 SKTVADFFNKGVYDQIVKMKGLPFMEAHAEPYMRQLIAGGV--ASGPLITLSGIEKVENV 553
           +KTV D FN  +Y+ I+ +KGLPF+EA+ EP+MR L  G +  A  P++TL+G+EKV N+
Sbjct: 422 AKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANI 481

Query: 554 MQALTMTNHNGFPVVDEPPFSDSSELCGLVLRSHLLVLLKGKKF-TKQRVSIRSGIMGSF 613
           +  L  T HN FPV+D    +  +EL GL+LR+HL+ +LK + F  ++R +    +   F
Sbjct: 482 VDVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKF 541

Query: 614 KAHDFAKAGSGKGVKLEDLDIDEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHGLG 673
              + A+    +    +D+ I   EM+++VDLHP+TNT+PYTVV++MS+AKA +LF  +G
Sbjct: 542 TPVELAE----REDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFRSVG 601

Query: 674 LRHLLVVPK--TPGRPPITGILTRHDFMPEHVLGLYPHLNPHK 712
           LRHLLVVPK    G  P+ GILTR D    ++L  +PHL+ HK
Sbjct: 602 LRHLLVVPKIQASGMSPVIGILTRQDLRAYNILQAFPHLDKHK 638

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136814.10.0e+0099.86chloride channel protein CLC-c [Momordica charantia][more]
XP_023552785.10.0e+0090.58chloride channel protein CLC-c-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022929418.10.0e+0090.44chloride channel protein CLC-c-like isoform X1 [Cucurbita moschata] >KAG6577357.... [more]
XP_022984849.10.0e+0090.44chloride channel protein CLC-c-like isoform X1 [Cucurbita maxima][more]
XP_008452224.10.0e+0090.58PREDICTED: LOW QUALITY PROTEIN: chloride channel protein CLC-c [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q962820.0e+0077.11Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=... [more]
P603004.1e-26661.04Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=... [more]
P929416.1e-22254.89Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=... [more]
P929421.0e-22154.99Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=... [more]
P929434.0e-17348.73Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1C5E70.0e+0099.86chloride channel protein CLC-c OS=Momordica charantia OX=3673 GN=LOC111008418 PE... [more]
A0A6J1ES180.0e+0090.44Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111435995 PE=3 SV=1[more]
A0A6J1JBP40.0e+0090.44Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111483006 PE=3 SV=1[more]
A0A1S3BUH50.0e+0090.58LOW QUALITY PROTEIN: chloride channel protein CLC-c OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1FPV10.0e+0090.42chloride channel protein CLC-c-like OS=Cucurbita moschata OX=3662 GN=LOC11144771... [more]
Match NameE-valueIdentityDescription
AT5G49890.10.0e+0077.11chloride channel C [more]
AT5G33280.12.9e-26761.04Voltage-gated chloride channel family protein [more]
AT5G40890.14.4e-22354.89chloride channel A [more]
AT3G27170.17.4e-22354.99chloride channel B [more]
AT5G40890.24.4e-19954.90chloride channel A [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002251Chloride channel ClC-plantPRINTSPR01120CLCHANNELPLTcoord: 225..235
score: 81.82
coord: 367..383
score: 74.51
coord: 243..250
score: 73.61
coord: 211..219
score: 70.37
IPR001807Chloride channel, voltage gatedPRINTSPR00762CLCHANNELcoord: 449..465
score: 53.53
coord: 467..486
score: 47.8
coord: 505..519
score: 38.53
coord: 126..145
score: 50.1
coord: 190..209
score: 67.4
coord: 95..112
score: 65.67
coord: 415..435
score: 59.24
IPR001807Chloride channel, voltage gatedPFAMPF00654Voltage_CLCcoord: 82..499
e-value: 9.8E-95
score: 317.8
IPR000644CBS domainPFAMPF00571CBScoord: 647..695
e-value: 0.0034
score: 17.8
coord: 538..591
e-value: 0.0065
score: 16.9
NoneNo IPR availableGENE3D1.10.3080.10Clc chloride channelcoord: 18..518
e-value: 1.5E-129
score: 434.7
NoneNo IPR availableGENE3D3.10.580.10coord: 519..701
e-value: 6.2E-7
score: 31.1
NoneNo IPR availablePANTHERPTHR11689CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBERcoord: 1..711
NoneNo IPR availablePANTHERPTHR11689:SF152CHLORIDE CHANNEL PROTEIN CLC-Ccoord: 1..711
NoneNo IPR availableCDDcd03685ClC_6_likecoord: 1..518
e-value: 0.0
score: 572.289
NoneNo IPR availableCDDcd04591CBS_pair_voltage-gated_CLC_euk_baccoord: 521..698
e-value: 1.02278E-32
score: 120.317
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 522..696
IPR014743Chloride channel, coreSUPERFAMILY81340Clc chloride channelcoord: 4..520

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012557.1MS012557.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006821 chloride transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0005247 voltage-gated chloride channel activity