MS012152 (gene) Bitter gourd (TR) v1

Overview


Name	MS012152
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	exopolygalacturonase clone GBGE184-like
Location	scaffold708: 679014 .. 679820 (+)
RNA-Seq Expression	MS012152
Synteny	MS012152

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGGTATTCAATCTTTTCCCATTTGAAATAATATTATATGCAAATTTTGTGCTAACATATGCAGATTTTGCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTCATGGGAGTTACTGTCACAAATTGCACATTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTAAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCGTAAGTATTAGAACAAGCAACAAGAACAACGTTCCATAAAGACTTCGTGTTATAACGATCATGACCATGTTATTATATCTTACAGTGAGTTTGAAGTATTATTATTAATGTTGTTCATGTGTTTGATATGCATGCAGCCATCTCAAGTGAAGATAAGCGAGGTAAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

mRNA sequence

AACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTCATGGGAGTTACTGTCACAAATTGCACATTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTAAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCCCATCTCAAGTGAAGATAAGCGAGGTAAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

Coding sequence (CDS)

AACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTCATGGGAGTTACTGTCACAAATTGCACATTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTAAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCCCATCTCAAGTGAAGATAAGCGAGGTAAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

Protein sequence

NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPAC

Homology

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BLAST of MS012152 vs. NCBI nr
Match: XP_022146404.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 377.5 bits (968), Expect = 7.2e-101
Identity = 184/200 (92.00%), Postives = 195/200 (97.50%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIHTSESNLINITNT+ISTGDDCVSIGQGTTNI+VHNVTCAPGHG+SVGSLGKY+DE
Sbjct: 238 NTDGIHTSESNLINITNTFISTGDDCVSIGQGTTNIHVHNVTCAPGHGLSVGSLGKYKDE 297

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           KDVMGVT+TNCTLRNTTNGLRIKTWADS PTQASKISFQDIILDAVKNPIIIDQSYGSKD
Sbjct: 298 KDVMGVTITNCTLRNTTNGLRIKTWADSPPTQASKISFQDIILDAVKNPIIIDQSYGSKD 357

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           KSKPPSQVKISEVNYKN+RGTTISVVPVSLQCS KVPCEG+KLEEID+SFVGS+KK  AF
Sbjct: 358 KSKPPSQVKISEVNYKNVRGTTISVVPVSLQCSPKVPCEGIKLEEIDLSFVGSDKKMTAF 417

Query: 181 GNSCLNAKITTVGKQIPPAC 201
            N+C+NAKITTVGKQIPPAC
Sbjct: 418 ANACVNAKITTVGKQIPPAC 437

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BLAST of MS012152 vs. NCBI nr
Match: XP_022153738.1 (exopolygalacturonase clone GBGE184-like, partial [Momordica charantia])

HSP 1 Score: 362.1 bits (928), Expect = 3.1e-96
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 20  ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVMGVTVTNCTLRNTTNG 79
           ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDV+GVTVTNCTLRNTTNG
Sbjct: 1   ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTVTNCTLRNTTNG 60

Query: 80  LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR 139
           LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR
Sbjct: 61  LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR 120

Query: 140 GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA 199
           GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA
Sbjct: 121 GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA 180

Query: 200 C 201
           C
Sbjct: 181 C 181

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BLAST of MS012152 vs. NCBI nr
Match: XP_038899827.1 (exopolygalacturonase clone GBGE184 [Benincasa hispida])

HSP 1 Score: 294.7 bits (753), Expect = 6.2e-76
Identity = 144/200 (72.00%), Postives = 170/200 (85.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH S+++++NITN+ I TGDDCVSIG G+TNI V NVTC PGHGISVGSLGKY DE
Sbjct: 233 NTDGIHISQTDVVNITNSIIGTGDDCVSIGHGSTNITVSNVTCGPGHGISVGSLGKYDDE 292

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNGLRIKTWA S   QA++I+FQDIILD+V+NPIIIDQ+YGSK 
Sbjct: 293 KEVRGIFVSNCTIRNTTNGLRIKTWAASPAGQATRITFQDIILDSVRNPIIIDQTYGSK- 352

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K K PSQVKIS+V +KNIRGTT+S V VSLQCSA VPC+G+KLEEID++F G+  K   F
Sbjct: 353 KKKAPSQVKISDVVFKNIRGTTVSDVGVSLQCSAAVPCDGIKLEEIDLAFTGTRLKHPTF 412

Query: 181 GNSCLNAKITTVGKQIPPAC 201
            NSCLNAKIT +GKQ PPAC
Sbjct: 413 ANSCLNAKITAIGKQNPPAC 431

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BLAST of MS012152 vs. NCBI nr
Match: KAA0046656.1 (exopolygalacturonase clone [Cucumis melo var. makuwa])

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 141/200 (70.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDG+H S+++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DE
Sbjct: 231 NTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDE 290

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK 
Sbjct: 291 KEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK- 350

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K + PSQVKIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS + K  F
Sbjct: 351 KKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPF 410

Query: 181 GNSCLNAKITTVGKQIPPAC 201
           GNSCLNAKITT+GKQ PPAC
Sbjct: 411 GNSCLNAKITTIGKQNPPAC 428

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BLAST of MS012152 vs. NCBI nr
Match: XP_008451547.1 (PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopolygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 141/200 (70.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDG+H S+++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DE
Sbjct: 231 NTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDE 290

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK 
Sbjct: 291 KEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK- 350

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K + PSQVKIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS + K  F
Sbjct: 351 KKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPF 410

Query: 181 GNSCLNAKITTVGKQIPPAC 201
           GNSCLNAKITT+GKQ PPAC
Sbjct: 411 GNSCLNAKITTIGKQNPPAC 428

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BLAST of MS012152 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.5e-51
Identity = 104/206 (50.49%), Postives = 144/206 (69.90%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH S ++ ++I ++ I+TGDDCVS+G+G+ N+ V  V C PGHG+SVGSLGKY++E
Sbjct: 218 NTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNE 277

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           +DV G+ V NCT+  T NGLRIKTW  S P++A  I F++II+ +VKNPIIIDQ+YGS+ 
Sbjct: 278 EDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRG 337

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFV---GSEKKK 180
                SQV IS++ +KNIRGTTI+   V + CS  VPC+GV + ++++ +V   G EKK 
Sbjct: 338 GD---SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKS 397

Query: 181 AA---FGNSCLNAKITTVGKQIPPAC 201
           ++    G  C NA +   GK   P C
Sbjct: 398 SSGGLVGALCDNANVIFGGKLSFPMC 420

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BLAST of MS012152 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.5e-51
Identity = 100/202 (49.50%), Postives = 141/202 (69.80%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   S+ +NI NT I TGDDC+S+G G+ NIN+ N+TC PGHGISVGSLG+Y++E
Sbjct: 156 NTDGIHIGRSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNE 215

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           + V+G+ V NCT+  + NG+RIKTW  S P +AS++ FQDI +++V  PI+IDQ Y   +
Sbjct: 216 ESVVGIYVKNCTITGSQNGVRIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYN 275

Query: 121 K--SKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
           +  ++ PS VK+S++++KNI+GT+ +   V L CS   PC GV+L +ID+++ G   K  
Sbjct: 276 QCTAEVPSSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSG---KGG 335

Query: 181 AFGNSCLNAKITTVGKQIPPAC 201
              + C N K T  GKQIP  C
Sbjct: 336 PATSVCENIKPTIKGKQIPAIC 354

[Close]

BLAST of MS012152 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.5e-48
Identity = 102/202 (50.50%), Postives = 136/202 (67.33%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH S S+ +NIT++  STGDDC+S+G  T  + +  VTC PGHGISVGSLG   DE
Sbjct: 190 NTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDE 249

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K V+GV V NCT  NT NG+RIKTW  S P   + + F+DII+  V NP++IDQ Y   +
Sbjct: 250 KPVVGVFVRNCTFTNTDNGVRIKTWPASHPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFN 309

Query: 121 KSKP--PSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
           K     PSQVKIS+V+++NI+GT+ +   VSL  S  VPCEG+++ +ID+++ G   K+ 
Sbjct: 310 KCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSG---KEG 369

Query: 181 AFGNSCLNAKITTVGKQIPPAC 201
              +SC N K +  GKQ PP C
Sbjct: 370 PAKSSCENIKPSLKGKQNPPVC 388

[Close]

BLAST of MS012152 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.4e-48
Identity = 92/199 (46.23%), Postives = 134/199 (67.34%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   S  + ITNT I+TGDDC+SIG G+ N+ +  V C PGHGIS+GSLG+Y +E
Sbjct: 177 NTDGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNE 236

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GS 120
           K+V G+TV  CT   T NG+R+KTW +S P  A+ ++FQD+ ++ V+NP+I+DQ Y    
Sbjct: 237 KEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYG 296

Query: 121 KDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
           +   + PS++K+S +N+ NIRGT+   V V + CS  +PC  +K+ EI++S+ G+     
Sbjct: 297 QCSRQAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPAT 356

Query: 181 AFGNSCLNAKITTVGKQIP 198
              ++C N K T  GKQ+P
Sbjct: 357 ---STCSNVKPTFSGKQVP 372

[Close]

BLAST of MS012152 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 8.2e-47
Identity = 100/202 (49.50%), Postives = 127/202 (62.87%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   SN +N+    I TGDDCVSIG GT N+ V NV C PGHGIS+GSLG+Y +E
Sbjct: 238 NTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNE 297

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GS 120
           + V GVTV  C ++NT NG+RIKTW  S P  AS I F+DI +D V  P++IDQ Y    
Sbjct: 298 QPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYG 357

Query: 121 KDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
             K+  PSQVK+S+V  K I+GT+ + V V L CS  VPC  + L +I++   G E    
Sbjct: 358 HCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAV 417

Query: 181 AFGNSCLNAKITTVGKQIPPAC 201
              ++C N K    GK +P AC
Sbjct: 418 ---SACSNIKPILSGKLVPAAC 436

[Close]

BLAST of MS012152 vs. ExPASy TrEMBL
Match: A0A6J1CY21 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111015625 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.5e-101
Identity = 184/200 (92.00%), Postives = 195/200 (97.50%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIHTSESNLINITNT+ISTGDDCVSIGQGTTNI+VHNVTCAPGHG+SVGSLGKY+DE
Sbjct: 238 NTDGIHTSESNLINITNTFISTGDDCVSIGQGTTNIHVHNVTCAPGHGLSVGSLGKYKDE 297

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           KDVMGVT+TNCTLRNTTNGLRIKTWADS PTQASKISFQDIILDAVKNPIIIDQSYGSKD
Sbjct: 298 KDVMGVTITNCTLRNTTNGLRIKTWADSPPTQASKISFQDIILDAVKNPIIIDQSYGSKD 357

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           KSKPPSQVKISEVNYKN+RGTTISVVPVSLQCS KVPCEG+KLEEID+SFVGS+KK  AF
Sbjct: 358 KSKPPSQVKISEVNYKNVRGTTISVVPVSLQCSPKVPCEGIKLEEIDLSFVGSDKKMTAF 417

Query: 181 GNSCLNAKITTVGKQIPPAC 201
            N+C+NAKITTVGKQIPPAC
Sbjct: 418 ANACVNAKITTVGKQIPPAC 437

[Close]

BLAST of MS012152 vs. ExPASy TrEMBL
Match: A0A6J1DI97 (exopolygalacturonase clone GBGE184-like OS=Momordica charantia OX=3673 GN=LOC111021182 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.5e-96
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 20  ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVMGVTVTNCTLRNTTNG 79
           ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDV+GVTVTNCTLRNTTNG
Sbjct: 1   ISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTVTNCTLRNTTNG 60

Query: 80  LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR 139
           LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR
Sbjct: 61  LRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIR 120

Query: 140 GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA 199
           GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA
Sbjct: 121 GTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPA 180

Query: 200 C 201
           C
Sbjct: 181 C 181

[Close]

BLAST of MS012152 vs. ExPASy TrEMBL
Match: A0A5D3D1R6 (Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001270 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.1e-76
Identity = 141/200 (70.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDG+H S+++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DE
Sbjct: 231 NTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDE 290

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK 
Sbjct: 291 KEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK- 350

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K + PSQVKIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS + K  F
Sbjct: 351 KKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPF 410

Query: 181 GNSCLNAKITTVGKQIPPAC 201
           GNSCLNAKITT+GKQ PPAC
Sbjct: 411 GNSCLNAKITTIGKQNPPAC 428

[Close]

BLAST of MS012152 vs. ExPASy TrEMBL
Match: A0A5A7TXI9 (Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G00200 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.1e-76
Identity = 141/200 (70.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDG+H S+++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DE
Sbjct: 231 NTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDE 290

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK 
Sbjct: 291 KEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK- 350

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K + PSQVKIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS + K  F
Sbjct: 351 KKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPF 410

Query: 181 GNSCLNAKITTVGKQIPPAC 201
           GNSCLNAKITT+GKQ PPAC
Sbjct: 411 GNSCLNAKITTIGKQNPPAC 428

[Close]

BLAST of MS012152 vs. ExPASy TrEMBL
Match: A0A1S3BRS3 (exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.1e-76
Identity = 141/200 (70.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDG+H S+++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DE
Sbjct: 231 NTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDE 290

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           K+V G+ V+NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK 
Sbjct: 291 KEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK- 350

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAF 180
           K + PSQVKIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS + K  F
Sbjct: 351 KKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPF 410

Query: 181 GNSCLNAKITTVGKQIPPAC 201
           GNSCLNAKITT+GKQ PPAC
Sbjct: 411 GNSCLNAKITTIGKQNPPAC 428

[Close]

BLAST of MS012152 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-52
Identity = 104/206 (50.49%), Postives = 144/206 (69.90%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH S ++ ++I ++ I+TGDDCVS+G+G+ N+ V  V C PGHG+SVGSLGKY++E
Sbjct: 218 NTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNE 277

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD 120
           +DV G+ V NCT+  T NGLRIKTW  S P++A  I F++II+ +VKNPIIIDQ+YGS+ 
Sbjct: 278 EDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRG 337

Query: 121 KSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFV---GSEKKK 180
                SQV IS++ +KNIRGTTI+   V + CS  VPC+GV + ++++ +V   G EKK 
Sbjct: 338 GD---SQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKS 397

Query: 181 AA---FGNSCLNAKITTVGKQIPPAC 201
           ++    G  C NA +   GK   P C
Sbjct: 398 SSGGLVGALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of MS012152 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 5.2e-49
Identity = 103/211 (48.82%), Postives = 143/211 (67.77%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   S+ +  + ++I+TGDDCVSIGQG + I + ++ C PGHGISVGSLG+Y +E
Sbjct: 203 NTDGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNE 262

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRP-TQASKISFQDIILDAVKNPIIIDQSY--G 120
           KDV G+ V +C +  TTNG+RIKTWA+S   + A+ ++F++II++ V NPIIIDQSY   
Sbjct: 263 KDVNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPF 322

Query: 121 SKDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDM---SFVGSE 180
           S   S  PS+V++SE+ +KNIRGT+ S+V V L CS  +PC+ V LE + +   S  G  
Sbjct: 323 SSCISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGR 382

Query: 181 KKKAAFGN-----SCLNAKITTVGKQIPPAC 201
           K+ +  GN     SC N +   +G QIPP C
Sbjct: 383 KQSSNRGNEAVSSSCRNVRANYIGTQIPPPC 413

[Close]

BLAST of MS012152 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 3.4e-48
Identity = 100/202 (49.50%), Postives = 128/202 (63.37%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   SN +N+    I TGDDCVSIG GT N+ V NV C PGHGIS+GSLG+Y +E
Sbjct: 239 NTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNE 298

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GS 120
           + V GVTV  C ++NT NG+RIKTW  S P  AS I F+DI +D V  P++IDQ Y    
Sbjct: 299 QPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYG 358

Query: 121 KDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
             K+  PS+VK+S+V  KNI+GT+ + V V L CS  VPC  + L +I++   G E    
Sbjct: 359 HCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAV 418

Query: 181 AFGNSCLNAKITTVGKQIPPAC 201
              ++C N K    GK +P AC
Sbjct: 419 ---SACSNIKPILSGKLVPAAC 437

[Close]

BLAST of MS012152 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 5.8e-48
Identity = 100/202 (49.50%), Postives = 127/202 (62.87%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   SN +N+    I TGDDCVSIG GT N+ V NV C PGHGIS+GSLG+Y +E
Sbjct: 238 NTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNE 297

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GS 120
           + V GVTV  C ++NT NG+RIKTW  S P  AS I F+DI +D V  P++IDQ Y    
Sbjct: 298 QPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYG 357

Query: 121 KDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKA 180
             K+  PSQVK+S+V  K I+GT+ + V V L CS  VPC  + L +I++   G E    
Sbjct: 358 HCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAV 417

Query: 181 AFGNSCLNAKITTVGKQIPPAC 201
              ++C N K    GK +P AC
Sbjct: 418 ---SACSNIKPILSGKLVPAAC 436

[Close]

BLAST of MS012152 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-46
Identity = 98/203 (48.28%), Postives = 132/203 (65.02%), Query Frame = 0


Query: 1   NTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDE 60
           NTDGIH   S+ + I N++ISTGDDC+S+G G  N++V  VTC PGHGISVGSLG+Y  E
Sbjct: 187 NTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHE 246

Query: 61  KDVMGVTVTNCTLRNTTNGLRIKTWADSR-PTQASKISFQDIILDAVKNPIIIDQSY--G 120
           +DV G+ V NCTL+ T NGLRIKTW  +   T AS I F+DIIL  V NPI+IDQ Y   
Sbjct: 247 QDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPW 306

Query: 121 SKDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKK 180
           ++   +  S +K+  +++KNIRGT+ +   V L CS   PC+ V++ +ID+ + G++   
Sbjct: 307 NQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGAD-GP 366

Query: 181 AAFGNSCLNAKITTVGKQIPPAC 201
           A F   C N     +G Q P AC
Sbjct: 367 ATF--HCSNVSPKILGSQSPKAC 386

The following BLAST results are available for this feature:

BLAST of MS012152 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146404.1	7.2e-101	92.00	exopolygalacturonase-like [Momordica charantia]	[more]
XP_022153738.1	3.1e-96	99.45	exopolygalacturonase clone GBGE184-like, partial [Momordica charantia]	[more]
XP_038899827.1	6.2e-76	72.00	exopolygalacturonase clone GBGE184 [Benincasa hispida]	[more]
KAA0046656.1	1.1e-75	70.50	exopolygalacturonase clone [Cucumis melo var. makuwa]	[more]
XP_008451547.1	1.1e-75	70.50	PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopoly...	[more]

BLAST of MS012152 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	2.5e-51	50.49	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
P24548	2.5e-51	49.50	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q05967	1.5e-48	50.50	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
Q6H9K0	7.4e-48	46.23	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P49063	8.2e-47	49.50	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]

BLAST of MS012152 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY21	3.5e-101	92.00	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111015625 PE=3 SV...	[more]
A0A6J1DI97	1.5e-96	99.45	exopolygalacturonase clone GBGE184-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3D1R6	5.1e-76	70.50	Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411...	[more]
A0A5A7TXI9	5.1e-76	70.50	Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BRS3	5.1e-76	70.50	exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 ...	[more]

BLAST of MS012152 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02790.1	1.7e-52	50.49	polygalacturonase 4	[more]
AT4G18180.1	5.2e-49	48.82	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	3.4e-48	49.50	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	5.8e-48	49.50	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	1.4e-46	48.28	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 10..31 e-value: 1800.0 score: 5.4 coord: 63..84 e-value: 0.45 score: 19.6 coord: 33..53 e-value: 7600.0 score: 0.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 1..176 e-value: 1.3E-59 score: 201.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 40..53
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..197 e-value: 2.5E-60 score: 206.6
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..197
None	No IPR available	PANTHER	PTHR31375:SF117	EXOPOLYGALACTURONASE-LIKE	coord: 1..197
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 1..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012152.1	MS012152.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity