MS012074 (gene) Bitter gourd (TR) v1

Overview


Name	MS012074
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein NIM1-INTERACTING 1
Location	scaffold708: 97481 .. 97861 (+)
RNA-Seq Expression	MS012074
Synteny	MS012074

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACGAGAACCTCCGGCCGGCGGCGGCGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTCGCTCTGTTGCGGAATTTTAAGGAAATGCGTGATCGCCGGAGGAAGGAGCTGACGACGATGATCGGCGACGGCGGAGAAACCGAGAAGAAGAGGAAGAAGATGAAGACGGCGCCGTCGACTTGGATTCCGACGTTTCTGAGAGAGGATTTCGATGAGGATTTTCAGGTTAGAGGACCGCCTCCGGTGCTTCCGAAGCCCTGCAATCTTATCAAGGAAGACACGCCGCCGCCGCCGCCGCTGCCGGCAGCGGTGGCGAAGAAGAAGAATATGAAGAATGATGAGGAGTGTTTGGATCTTAATTTGGCGCTG

mRNA sequence

ATGGAGAACGAGAACCTCCGGCCGGCGGCGGCGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTCGCTCTGTTGCGGAATTTTAAGGAAATGCGTGATCGCCGGAGGAAGGAGCTGACGACGATGATCGGCGACGGCGGAGAAACCGAGAAGAAGAGGAAGAAGATGAAGACGGCGCCGTCGACTTGGATTCCGACGTTTCTGAGAGAGGATTTCGATGAGGATTTTCAGGTTAGAGGACCGCCTCCGGTGCTTCCGAAGCCCTGCAATCTTATCAAGGAAGACACGCCGCCGCCGCCGCCGCTGCCGGCAGCGGTGGCGAAGAAGAAGAATATGAAGAATGATGAGGAGTGTTTGGATCTTAATTTGGCGCTG

Coding sequence (CDS)

ATGGAGAACGAGAACCTCCGGCCGGCGGCGGCGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTCGCTCTGTTGCGGAATTTTAAGGAAATGCGTGATCGCCGGAGGAAGGAGCTGACGACGATGATCGGCGACGGCGGAGAAACCGAGAAGAAGAGGAAGAAGATGAAGACGGCGCCGTCGACTTGGATTCCGACGTTTCTGAGAGAGGATTTCGATGAGGATTTTCAGGTTAGAGGACCGCCTCCGGTGCTTCCGAAGCCCTGCAATCTTATCAAGGAAGACACGCCGCCGCCGCCGCCGCTGCCGGCAGCGGTGGCGAAGAAGAAGAATATGAAGAATGATGAGGAGTGTTTGGATCTTAATTTGGCGCTG

Protein sequence

MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKTAPSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKKNMKNDEECLDLNLAL

Homology

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BLAST of MS012074 vs. NCBI nr
Match: XP_022151003.1 (protein NIM1-INTERACTING 1 [Momordica charantia])

HSP 1 Score: 245.7 bits (626), Expect = 2.1e-61
Identity = 126/127 (99.21%), Postives = 126/127 (99.21%), Query Frame = 0


Query: 1   MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT 60
           MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT
Sbjct: 1   MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT 60

Query: 61  APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKKNMKNDEEC 120
           APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDT PPPPLPAAVAKKKNMKNDEEC
Sbjct: 61  APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDT-PPPPLPAAVAKKKNMKNDEEC 120

Query: 121 LDLNLAL 128
           LDLNLAL
Sbjct: 121 LDLNLAL 126

[Close]

BLAST of MS012074 vs. NCBI nr
Match: XP_038898983.1 (protein NIM1-INTERACTING 1-like [Benincasa hispida])

HSP 1 Score: 138.3 bits (347), Expect = 4.7e-29
Identity = 87/139 (62.59%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 1   MENENLRPAAAE--EEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGE---TEKKR 60
           MENE L+PA     EEEEE +ME FFAL+RNFKEMRDRRRKEL  MIG+  E    EK+R
Sbjct: 1   MENEKLQPAPERIAEEEEEEEMEMFFALVRNFKEMRDRRRKEL--MIGEEEEIAAEEKRR 60

Query: 61  KKMKTAP------STWIPTFLREDFDEDFQVRGPPPVLP-KPCNLIKEDTPPPPPLPAAV 120
           K+MK+AP      STWIP F REDFDE+FQVRG   +LP KP NL+KEDT       AA+
Sbjct: 61  KRMKSAPEAVENRSTWIPKFEREDFDEEFQVRGQGFILPNKPYNLVKEDTSAAAASTAAM 120

Query: 121 AKKKNMKNDEECLDLNLAL 128
            KKKN+KN ++CLDLNLAL
Sbjct: 121 KKKKNVKNGDDCLDLNLAL 137

[Close]

BLAST of MS012074 vs. NCBI nr
Match: XP_022991628.1 (uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991629.1 uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991630.1 uncharacterized protein LOC111488189 [Cucurbita maxima])

HSP 1 Score: 112.1 bits (279), Expect = 3.6e-21
Identity = 70/135 (51.85%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 1   MENENLRP---AAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKK 60
           MEN+  +P     AEEEEEE++ME F+AL+RNFKE+RDR RKE+    GD    EK RK+
Sbjct: 1   MENQKRQPPRETIAEEEEEEVEMEKFYALVRNFKEIRDRWRKEVVNGEGDETAAEKTRKR 60

Query: 61  MKTA-----PSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKK 120
           MKT       S+WI  F REDFDED QV G    LPK C L+KEDT   P   A + K+K
Sbjct: 61  MKTTETVQNQSSWILRFEREDFDEDLQVGGAAVELPKHCCLVKEDT---PVAAAPLKKEK 120

Query: 121 NMKNDEECLDLNLAL 128
            + +  +CLDLNL+L
Sbjct: 121 KVASGVDCLDLNLSL 132

[Close]

BLAST of MS012074 vs. NCBI nr
Match: XP_023514605.1 (protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.7 bits (278), Expect = 4.7e-21
Identity = 74/142 (52.11%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MENENLRPAA---------AEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGE- 60
           ME ++L+P            EEEEEEMKMEAFFAL+RNFKEMRD+RRKEL  MIG  GE 
Sbjct: 1   MEKQSLKPTGKRVAVAAVDGEEEEEEMKMEAFFALVRNFKEMRDQRRKEL--MIGCDGEK 60

Query: 61  ---TEKKRKKMKTAP--STWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLP 120
               E +++KMKT    S WIP F REDFDE+FQ+RGP           KE+        
Sbjct: 61  AAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQLRGP----------AKEEA------A 120

Query: 121 AAVAKKKNMKNDEECLDLNLAL 128
           AA  KKK +KN+E+ LDLNL+L
Sbjct: 121 AAAKKKKKVKNEEDILDLNLSL 124

[Close]

BLAST of MS012074 vs. NCBI nr
Match: XP_023547822.1 (uncharacterized protein LOC111806674 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.6 bits (270), Expect = 4.0e-20
Identity = 73/141 (51.77%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 1   MENENLRP---AAAEEE----EEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGET-- 60
           MEN+  +P     AEEE    EEE++ME F+AL+RNFKE+RDR RKE+    G+GGET  
Sbjct: 1   MENQKRQPPRGTIAEEEEAEAEEEVEMETFYALVRNFKEIRDRCRKEVVN--GEGGETAA 60

Query: 61  EKKRKKMKTA-----PSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPA 120
           EK RK+MKT       S+WI  F REDFDED QV G    LPK C L+KEDT  P   P 
Sbjct: 61  EKTRKRMKTTETSQNQSSWILRFEREDFDEDLQVGGTAVELPKRCCLVKEDT--PVAAPP 120

Query: 121 AVAKKKNMKNDEECLDLNLAL 128
           ++ K+K + +  +CLDLNL+L
Sbjct: 121 SMKKEKKVASGVDCLDLNLSL 137

[Close]

BLAST of MS012074 vs. ExPASy TrEMBL
Match: A0A6J1DAZ5 (protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.0e-61
Identity = 126/127 (99.21%), Postives = 126/127 (99.21%), Query Frame = 0


Query: 1   MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT 60
           MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT
Sbjct: 1   MENENLRPAAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKKMKT 60

Query: 61  APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKKNMKNDEEC 120
           APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDT PPPPLPAAVAKKKNMKNDEEC
Sbjct: 61  APSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDT-PPPPLPAAVAKKKNMKNDEEC 120

Query: 121 LDLNLAL 128
           LDLNLAL
Sbjct: 121 LDLNLAL 126

[Close]

BLAST of MS012074 vs. ExPASy TrEMBL
Match: A0A6J1JVD5 (uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-21
Identity = 70/135 (51.85%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 1   MENENLRP---AAAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGETEKKRKK 60
           MEN+  +P     AEEEEEE++ME F+AL+RNFKE+RDR RKE+    GD    EK RK+
Sbjct: 1   MENQKRQPPRETIAEEEEEEVEMEKFYALVRNFKEIRDRWRKEVVNGEGDETAAEKTRKR 60

Query: 61  MKTA-----PSTWIPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKK 120
           MKT       S+WI  F REDFDED QV G    LPK C L+KEDT   P   A + K+K
Sbjct: 61  MKTTETVQNQSSWILRFEREDFDEDLQVGGAAVELPKHCCLVKEDT---PVAAAPLKKEK 120

Query: 121 NMKNDEECLDLNLAL 128
            + +  +CLDLNL+L
Sbjct: 121 KVASGVDCLDLNLSL 132

[Close]

BLAST of MS012074 vs. ExPASy TrEMBL
Match: A0A6J1KR57 (protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 4.3e-20
Identity = 68/121 (56.20%), Postives = 81/121 (66.94%), Query Frame = 0


Query: 12  EEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDG---GETEKKRKKMKTAP--STWI 71
           EE+EEEMKMEAFF L+RNFKEMRD+RRKEL  ++ DG      E ++KKMKT    S WI
Sbjct: 22  EEKEEEMKMEAFFDLVRNFKEMRDQRRKEL-MIVCDGEKAAAAEMRKKKMKTVENRSAWI 81

Query: 72  PTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKKNMKNDEECLDLNLA 128
           P F REDFDE+FQVRGP           KE+        AA AKKK +KN+E+ LDLNL+
Sbjct: 82  PRFEREDFDEEFQVRGP----------AKEE--------AAAAKKKKVKNEEDILDLNLS 123

[Close]

BLAST of MS012074 vs. ExPASy TrEMBL
Match: A0A6J1H831 (protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.6e-19
Identity = 69/121 (57.02%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 13  EEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGE----TEKKRKKMKTAP--STWI 72
           EEEEEMKMEAFFAL+RNFKEMRD+RRKEL  MIG  GE     E +++KMKT    S WI
Sbjct: 25  EEEEEMKMEAFFALVRNFKEMRDQRRKEL--MIGCDGEKAAAAEMRKRKMKTVENRSAWI 84

Query: 73  PTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPPLPAAVAKKKNMKNDEECLDLNLA 128
           P F REDFDE+FQVRGP           KE+        A   KKK +KN+E+ LDLNL+
Sbjct: 85  PRFEREDFDEEFQVRGP----------AKEEA--AAAAIATAKKKKKVKNEEDILDLNLS 131

[Close]

BLAST of MS012074 vs. ExPASy TrEMBL
Match: A0A0A0K5V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.8e-16
Identity = 69/141 (48.94%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   MENENLRPA---AAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGET----EK 60
           MEN  L+P+    AE+  E+ +ME FFAL+R+ KEMRD+RRKEL     +G E     EK
Sbjct: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60

Query: 61  KRKKMKTAP-----STWIPTFLREDFDEDFQVRGPPPVLPKP-CNLIKEDTPPPPPLPAA 120
           +RK+M+ A      STWIP F REDFDE+FQ     P  P P CNL+K DT        A
Sbjct: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLP--PNPCCNLVKRDTTAASTTTTA 120

Query: 121 VAK-KKNMKNDEECLDLNLAL 128
             K KK +KN +  LDLNLAL
Sbjct: 121 PMKNKKKVKNGDVSLDLNLAL 139

The following BLAST results are available for this feature:

BLAST of MS012074 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151003.1	2.1e-61	99.21	protein NIM1-INTERACTING 1 [Momordica charantia]	[more]
XP_038898983.1	4.7e-29	62.59	protein NIM1-INTERACTING 1-like [Benincasa hispida]	[more]
XP_022991628.1	3.6e-21	51.85	uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991629.1 uncharac...	[more]
XP_023514605.1	4.7e-21	52.11	protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo]	[more]
XP_023547822.1	4.0e-20	51.77	uncharacterized protein LOC111806674 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS012074 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS012074 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAZ5	1.0e-61	99.21	protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 S...	[more]
A0A6J1JVD5	1.7e-21	51.85	uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189...	[more]
A0A6J1KR57	4.3e-20	56.20	protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1	[more]
A0A6J1H831	1.6e-19	57.02	protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV...	[more]
A0A0A0K5V7	3.8e-16	48.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1	[more]

BLAST of MS012074 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031425	NPR1/NH1-interacting protein	PFAM	PF15699	NPR1_interact	coord: 12..124 e-value: 1.6E-19 score: 70.6
IPR031425	NPR1/NH1-interacting protein	PANTHER	PTHR33669	PROTEIN NEGATIVE REGULATOR OF RESISTANCE	coord: 9..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 83..107
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..113
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..61
None	No IPR available	PANTHER	PTHR33669:SF1	NPR1/NH1-INTERACTING PROTEIN-RELATED	coord: 9..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012074.1	MS012074.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010112	regulation of systemic acquired resistance