MS008153 (gene) Bitter gourd (TR) v1

BLAST of MS008153 vs. NCBI nr
Match: XP_022139394.1 (septum-promoting GTP-binding protein 1 [Momordica charantia])

HSP 1 Score: 598.2 bits (1541), Expect = 3.9e-167
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF 60
           KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF
Sbjct: 7   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF 66

Query: 61  RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG 120
           RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG
Sbjct: 67  RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG 126

Query: 121 DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP 180
           DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP
Sbjct: 127 DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP 186

Query: 181 LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT 240
           LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT
Sbjct: 187 LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT 246

Query: 241 IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 299
           IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF
Sbjct: 247 IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 304

BLAST of MS008153 vs. NCBI nr
Match: XP_004142559.1 (septum-promoting GTP-binding protein 1 [Cucumis sativus] >KGN66693.1 hypothetical protein Csa_007396 [Cucumis sativus])

HSP 1 Score: 493.0 bits (1268), Expect = 1.8e-135
Identity = 257/302 (85.10%), Postives = 266/302 (88.08%), Query Frame = 0

Query: 6   KPNAAIE-NHGAKLRRLPS-LFAAKLTHDA-AAAMTKLCRRLVRWRLLERVSLVRHFFRL 65
           KPNAA       K   LPS LF++KL HDA +AAMTKLCR+LVRWRLLERVSLVRHFFRL
Sbjct: 7   KPNAAAAIQTNPKFPHLPSLLFSSKLAHDATSAAMTKLCRKLVRWRLLERVSLVRHFFRL 66

Query: 66  IWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVPLKI 125
           +WD LF CSSG TP KYRRL   SPP   D G SPD PTTS G  SD DFDS DLV LKI
Sbjct: 67  VWDRLFACSSGTTPKKYRRLPSRSPPPPTDGGSSPDQPTTSGGFDSDFDFDSADLVSLKI 126

Query: 126 SLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQ 185
           SLLGDCQIGKTSFVIKYVGDEQER SLQM+GINLMNKTL+IQNALISF IWDVKGD SSQ
Sbjct: 127 SLLGDCQIGKTSFVIKYVGDEQERNSLQMSGINLMNKTLNIQNALISFCIWDVKGDSSSQ 186

Query: 186 DHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 245
           D LPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPP+
Sbjct: 187 DQLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPN 246

Query: 246 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPII 299
           LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFD+PWSIERNLTIGEPII
Sbjct: 247 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDIPWSIERNLTIGEPII 306

BLAST of MS008153 vs. NCBI nr
Match: KAG6607809.1 (hypothetical protein SDJN03_01151, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015207.1 spg1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.9 bits (1265), Expect = 3.9e-135
Identity = 251/305 (82.30%), Postives = 268/305 (87.87%), Query Frame = 0

Query: 1   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHD-AAAAMTKLCRRLVRWRLLERVSLVRHF 60
           K+N++      EN   K R LPSLF +KL ++ AAAAMTKLCRRLVRWRL+ER+S VRHF
Sbjct: 6   KSNAAATEINGENKN-KRRHLPSLFTSKLNNEAAAAAMTKLCRRLVRWRLIERISFVRHF 65

Query: 61  FRLIWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVP 120
           FR IWD +F CSS RTP KYRR    SPP   D G SP+ PTTS G  SDSDFDS +LVP
Sbjct: 66  FRFIWDRVFACSSARTPKKYRRFSHRSPPAATDGGSSPEYPTTSGGFDSDSDFDSAELVP 125

Query: 121 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 180
           LKISLLGDCQIGKTSFV+KYVGDEQER SLQM+GINLMNKTLSIQNALISFSIWDVKGD 
Sbjct: 126 LKISLLGDCQIGKTSFVVKYVGDEQERNSLQMSGINLMNKTLSIQNALISFSIWDVKGDS 185

Query: 181 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRL 240
           SSQD LPLACKDAVAILFMFDLTSRRTLNS+VDWYTEARKWNQTAIPILIGTKFDDFVRL
Sbjct: 186 SSQDQLPLACKDAVAILFMFDLTSRRTLNSIVDWYTEARKWNQTAIPILIGTKFDDFVRL 245

Query: 241 PPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGE 299
           PPDLQWTIVSQARAYARVMKATLFFSSS+HNINVNKIFKFIAAKLFDVPWS+ERNLTIGE
Sbjct: 246 PPDLQWTIVSQARAYARVMKATLFFSSSVHNINVNKIFKFIAAKLFDVPWSVERNLTIGE 305

BLAST of MS008153 vs. NCBI nr
Match: XP_008454520.1 (PREDICTED: septum-promoting GTP-binding protein 1 isoform X2 [Cucumis melo] >KAA0053099.1 septum-promoting GTP-binding protein 1 isoform X2 [Cucumis melo var. makuwa] >TYK29940.1 septum-promoting GTP-binding protein 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 491.1 bits (1263), Expect = 6.7e-135
Identity = 256/302 (84.77%), Postives = 265/302 (87.75%), Query Frame = 0

Query: 6   KPNAAIE-NHGAKLRRLPS-LFAAKLTHDA-AAAMTKLCRRLVRWRLLERVSLVRHFFRL 65
           KP+AA       K   LPS LF+AKL HDA AAAMTKLCR+LVRWRLLERVSLVRHFFR 
Sbjct: 7   KPHAAAAIQTNPKFPHLPSLLFSAKLAHDATAAAMTKLCRKLVRWRLLERVSLVRHFFRF 66

Query: 66  IWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVPLKI 125
           IWD LF CSSG TP +YRRL   SPP   D G SP+ PTTS    SDSDFDS DLV LKI
Sbjct: 67  IWDRLFSCSSGTTPKRYRRLPSRSPPPATDGGSSPEQPTTSGAFDSDSDFDSADLVSLKI 126

Query: 126 SLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQ 185
           SLLGDCQIGKTSFVIKYVGDEQER SLQM+GINLMNKTLSIQNALISF IWDVKGD SSQ
Sbjct: 127 SLLGDCQIGKTSFVIKYVGDEQERNSLQMSGINLMNKTLSIQNALISFCIWDVKGDSSSQ 186

Query: 186 DHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 245
           D LPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD
Sbjct: 187 DQLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 246

Query: 246 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPII 299
           LQWT+VSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFD+PWS+ERNLTIGEPII
Sbjct: 247 LQWTVVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDIPWSVERNLTIGEPII 306

BLAST of MS008153 vs. NCBI nr
Match: XP_023525753.1 (septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490.7 bits (1262), Expect = 8.7e-135
Identity = 250/305 (81.97%), Postives = 268/305 (87.87%), Query Frame = 0

Query: 1   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHD-AAAAMTKLCRRLVRWRLLERVSLVRHF 60
           K+N++      EN   K R LPSLF +KL ++ AAAAMTKLCRRLVRWRL+ER+S VRHF
Sbjct: 6   KSNAAATEINGENKN-KRRHLPSLFTSKLNNEAAAAAMTKLCRRLVRWRLIERISFVRHF 65

Query: 61  FRLIWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVP 120
           FR IWD +F CSS RTP KYRR    SPP   D G SP+ PTTS G  SDSDFDS +LVP
Sbjct: 66  FRFIWDRVFACSSARTPKKYRRFSNRSPPAAADGGSSPEYPTTSGGFDSDSDFDSAELVP 125

Query: 121 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 180
           LKISLLGDCQIGKTSFV+KYVGDEQER SLQM+GINLMNKTL+IQNALISFSIWDVKGD 
Sbjct: 126 LKISLLGDCQIGKTSFVVKYVGDEQERNSLQMSGINLMNKTLNIQNALISFSIWDVKGDS 185

Query: 181 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRL 240
           SSQD LPLACKDAVAILFMFDLTSRRTLNS+VDWYTEARKWNQTAIPILIGTKFDDFVRL
Sbjct: 186 SSQDQLPLACKDAVAILFMFDLTSRRTLNSIVDWYTEARKWNQTAIPILIGTKFDDFVRL 245

Query: 241 PPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGE 299
           PPDLQWTIVSQARAYARVMKATLFFSSS+HNINVNKIFKFIAAKLFDVPWS+ERNLTIGE
Sbjct: 246 PPDLQWTIVSQARAYARVMKATLFFSSSVHNINVNKIFKFIAAKLFDVPWSVERNLTIGE 305

BLAST of MS008153 vs. ExPASy Swiss-Prot
Match: P87027 (Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spg1 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 93/187 (49.73%), Postives = 131/187 (70.05%), Query Frame = 0

Query: 112 VPLKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKG 171
           V +K+ ++GD  IGKTS ++ YV    +  S Q  G+N M KT+SI+N  I+FSIWD+ G
Sbjct: 9   VTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGG 68

Query: 172 DRSSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFV 231
            R   + LP+ C DAVAILFMFDL+ + TLNS+ +WY +AR +N+TA+PILIGTK+D F+
Sbjct: 69  QREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYDHFM 128

Query: 232 RLPPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTI 291
             P + Q  I  QAR YA+ MKA+L F S+ H+INV KIFK + AK+FD+  +I     +
Sbjct: 129 TFPREDQEEITKQARRYAKAMKASLVFCSTSHSINVQKIFKIVLAKVFDLKCTIPEIKNV 188

Query: 292 GEPIIDF 299
           G+PI+++
Sbjct: 189 GDPILEY 195

BLAST of MS008153 vs. ExPASy Swiss-Prot
Match: P38987 (Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TEM1 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.7e-36
Identity = 74/185 (40.00%), Postives = 117/185 (63.24%), Query Frame = 0

Query: 112 VPLKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKG 171
           V +++ L+GD Q+GKTS ++KYV +  ++   Q  G+N + + +SI++  I FSI D+ G
Sbjct: 19  VEVQVGLVGDAQVGKTSLMVKYVQNIYDKEYTQTLGVNFLKRKVSIRSTDIIFSIMDLGG 78

Query: 172 DRSSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFV 231
            R   + LP+A   +  I+F+FDLT   TL+S+ +WY +A   N +AIPIL+GTK+D  +
Sbjct: 79  QREFINMLPIATVGSSVIIFLFDLTRPETLSSIKEWYRQAYGLNDSAIPILVGTKYDLLI 138

Query: 232 RLPPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTI 291
            L P+ Q  I   +  YA+VM A L F S+  +IN+ KIFK   AK+F++  +I     I
Sbjct: 139 DLDPEYQEQISRTSMKYAQVMNAPLIFCSTAKSINIQKIFKIALAKIFNLTLTIPEINEI 198

Query: 292 GEPII 297
           G+P++
Sbjct: 199 GDPLL 203

BLAST of MS008153 vs. ExPASy Swiss-Prot
Match: P51154 (Ras-related protein Rab-22A OS=Canis lupus familiaris OX=9615 GN=RAB22A PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.2e-15
Identity = 46/165 (27.88%), Postives = 84/165 (50.91%), Query Frame = 0

Query: 114 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 173
           LK+ LLGD  +GK+S V ++V D  +       G + M KT+  QN L  F IWD  G  
Sbjct: 6   LKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQE 65

Query: 174 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRL 233
           + +   P+  + + A + ++D+T   T +++ +W  E R+     I + I     D +  
Sbjct: 66  AFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVKELRQHGPPNIVVAIAGNKCDLI-- 125

Query: 234 PPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKL 279
             D++  +   A+ YA  + A    +S+ + IN+N++F  I+ ++
Sbjct: 126 --DVREVMERDAKDYADSIHAIFVETSAKNAININELFIEISRRI 166

BLAST of MS008153 vs. ExPASy Swiss-Prot
Match: Q9UL26 (Ras-related protein Rab-22A OS=Homo sapiens OX=9606 GN=RAB22A PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 46/165 (27.88%), Postives = 83/165 (50.30%), Query Frame = 0

Query: 114 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 173
           LK+ LLGD  +GK+S V ++V D  +       G + M KT+  QN L  F IWD  G  
Sbjct: 6   LKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQE 65

Query: 174 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRL 233
             +   P+  + + A + ++D+T   T +++ +W  E R+     I + I     D +  
Sbjct: 66  RFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVKELRQHGPPNIVVAIAGNKCDLI-- 125

Query: 234 PPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKL 279
             D++  +   A+ YA  + A    +S+ + IN+N++F  I+ ++
Sbjct: 126 --DVREVMERDAKDYADSIHAIFVETSAKNAININELFIEISRRI 166

BLAST of MS008153 vs. ExPASy Swiss-Prot
Match: P35285 (Ras-related protein Rab-22A OS=Mus musculus OX=10090 GN=Rab22a PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 48/166 (28.92%), Postives = 85/166 (51.20%), Query Frame = 0

Query: 114 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 173
           LK+ LLGD  +GK+S V ++V D  +       G + M KT+  QN L  F IWD  G  
Sbjct: 6   LKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQE 65

Query: 174 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILI-GTKFDDFVR 233
             +   P+  + + A + ++D+T   T +++ +W  E R+    +I + I G K D    
Sbjct: 66  RFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVRELRQHGPPSIVVAIAGNKCD---- 125

Query: 234 LPPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKL 279
              D++  +   A+ YA  + A    +S+ + IN+N++F  I+ ++
Sbjct: 126 -LTDVREVMERDAKDYADSIHAIFVETSAKNAININELFIEISRRI 166

BLAST of MS008153 vs. ExPASy TrEMBL
Match: A0A6J1CCV1 (septum-promoting GTP-binding protein 1 OS=Momordica charantia OX=3673 GN=LOC111010334 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.9e-167
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF 60
           KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF
Sbjct: 7   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHDAAAAMTKLCRRLVRWRLLERVSLVRHFF 66

Query: 61  RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG 120
           RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG
Sbjct: 67  RLIWDSLFVCSSGRTPGKYRRLSPPDAGLSPDDPTTSAGLHSDSDFDSDDLVPLKISLLG 126

Query: 121 DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP 180
           DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP
Sbjct: 127 DCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLP 186

Query: 181 LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT 240
           LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT
Sbjct: 187 LACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWT 246

Query: 241 IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 299
           IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF
Sbjct: 247 IVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 304

BLAST of MS008153 vs. ExPASy TrEMBL
Match: A0A0A0LY45 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G660130 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.5e-136
Identity = 257/302 (85.10%), Postives = 266/302 (88.08%), Query Frame = 0

Query: 6   KPNAAIE-NHGAKLRRLPS-LFAAKLTHDA-AAAMTKLCRRLVRWRLLERVSLVRHFFRL 65
           KPNAA       K   LPS LF++KL HDA +AAMTKLCR+LVRWRLLERVSLVRHFFRL
Sbjct: 7   KPNAAAAIQTNPKFPHLPSLLFSSKLAHDATSAAMTKLCRKLVRWRLLERVSLVRHFFRL 66

Query: 66  IWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVPLKI 125
           +WD LF CSSG TP KYRRL   SPP   D G SPD PTTS G  SD DFDS DLV LKI
Sbjct: 67  VWDRLFACSSGTTPKKYRRLPSRSPPPPTDGGSSPDQPTTSGGFDSDFDFDSADLVSLKI 126

Query: 126 SLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQ 185
           SLLGDCQIGKTSFVIKYVGDEQER SLQM+GINLMNKTL+IQNALISF IWDVKGD SSQ
Sbjct: 127 SLLGDCQIGKTSFVIKYVGDEQERNSLQMSGINLMNKTLNIQNALISFCIWDVKGDSSSQ 186

Query: 186 DHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 245
           D LPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPP+
Sbjct: 187 DQLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPN 246

Query: 246 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPII 299
           LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFD+PWSIERNLTIGEPII
Sbjct: 247 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDIPWSIERNLTIGEPII 306

BLAST of MS008153 vs. ExPASy TrEMBL
Match: A0A5D3E2Y7 (Septum-promoting GTP-binding protein 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G00010 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 256/302 (84.77%), Postives = 265/302 (87.75%), Query Frame = 0

Query: 6   KPNAAIE-NHGAKLRRLPS-LFAAKLTHDA-AAAMTKLCRRLVRWRLLERVSLVRHFFRL 65
           KP+AA       K   LPS LF+AKL HDA AAAMTKLCR+LVRWRLLERVSLVRHFFR 
Sbjct: 7   KPHAAAAIQTNPKFPHLPSLLFSAKLAHDATAAAMTKLCRKLVRWRLLERVSLVRHFFRF 66

Query: 66  IWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVPLKI 125
           IWD LF CSSG TP +YRRL   SPP   D G SP+ PTTS    SDSDFDS DLV LKI
Sbjct: 67  IWDRLFSCSSGTTPKRYRRLPSRSPPPATDGGSSPEQPTTSGAFDSDSDFDSADLVSLKI 126

Query: 126 SLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQ 185
           SLLGDCQIGKTSFVIKYVGDEQER SLQM+GINLMNKTLSIQNALISF IWDVKGD SSQ
Sbjct: 127 SLLGDCQIGKTSFVIKYVGDEQERNSLQMSGINLMNKTLSIQNALISFCIWDVKGDSSSQ 186

Query: 186 DHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 245
           D LPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD
Sbjct: 187 DQLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 246

Query: 246 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPII 299
           LQWT+VSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFD+PWS+ERNLTIGEPII
Sbjct: 247 LQWTVVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDIPWSVERNLTIGEPII 306

BLAST of MS008153 vs. ExPASy TrEMBL
Match: A0A1S3BZK2 (septum-promoting GTP-binding protein 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494916 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 256/302 (84.77%), Postives = 265/302 (87.75%), Query Frame = 0

Query: 6   KPNAAIE-NHGAKLRRLPS-LFAAKLTHDA-AAAMTKLCRRLVRWRLLERVSLVRHFFRL 65
           KP+AA       K   LPS LF+AKL HDA AAAMTKLCR+LVRWRLLERVSLVRHFFR 
Sbjct: 7   KPHAAAAIQTNPKFPHLPSLLFSAKLAHDATAAAMTKLCRKLVRWRLLERVSLVRHFFRF 66

Query: 66  IWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVPLKI 125
           IWD LF CSSG TP +YRRL   SPP   D G SP+ PTTS    SDSDFDS DLV LKI
Sbjct: 67  IWDRLFSCSSGTTPKRYRRLPSRSPPPATDGGSSPEQPTTSGAFDSDSDFDSADLVSLKI 126

Query: 126 SLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDRSSQ 185
           SLLGDCQIGKTSFVIKYVGDEQER SLQM+GINLMNKTLSIQNALISF IWDVKGD SSQ
Sbjct: 127 SLLGDCQIGKTSFVIKYVGDEQERNSLQMSGINLMNKTLSIQNALISFCIWDVKGDSSSQ 186

Query: 186 DHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 245
           D LPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD
Sbjct: 187 DQLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPD 246

Query: 246 LQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPII 299
           LQWT+VSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFD+PWS+ERNLTIGEPII
Sbjct: 247 LQWTVVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDIPWSVERNLTIGEPII 306

BLAST of MS008153 vs. ExPASy TrEMBL
Match: A0A6J1FS22 (septum-promoting GTP-binding protein 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446716 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.2e-134
Identity = 250/305 (81.97%), Postives = 267/305 (87.54%), Query Frame = 0

Query: 1   KNNSSKPNAAIENHGAKLRRLPSLFAAKLTHD-AAAAMTKLCRRLVRWRLLERVSLVRHF 60
           K+N++      EN   K R LPSLF +KL ++ AAAAMTKLCRRLVRWRL+ER+S VRHF
Sbjct: 6   KSNAAATEINGENKN-KRRHLPSLFTSKLNNEAAAAAMTKLCRRLVRWRLIERISFVRHF 65

Query: 61  FRLIWDSLFVCSSGRTPGKYRRL---SPP---DAGLSPDDPTTSAGLHSDSDFDSDDLVP 120
           FR IWD +F CSS RTP KYRR    SPP   D G SP+ PTTS G  SDSDFDS +LVP
Sbjct: 66  FRFIWDRVFACSSARTPKKYRRFSHRSPPAATDGGSSPEYPTTSGGFDSDSDFDSAELVP 125

Query: 121 LKISLLGDCQIGKTSFVIKYVGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGDR 180
           LKISLLGDCQIGKTSFV+KYVGDEQER SLQM+GINLMNKTLSIQNALISFSIWDVKGD 
Sbjct: 126 LKISLLGDCQIGKTSFVVKYVGDEQERNSLQMSGINLMNKTLSIQNALISFSIWDVKGDS 185

Query: 181 SSQDHLPLACKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRL 240
           SSQD LPLACKDAVAILFMFDLTSRRTLNS+VDWYTEARKWNQTAIPILIGTKFDDFVRL
Sbjct: 186 SSQDQLPLACKDAVAILFMFDLTSRRTLNSIVDWYTEARKWNQTAIPILIGTKFDDFVRL 245

Query: 241 PPDLQWTIVSQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGE 299
           PPDLQWTIVS ARAYARVMKATLFFSSS+HNINVNKIFKFIAAKLFDVPWS+ERNLTIGE
Sbjct: 246 PPDLQWTIVSLARAYARVMKATLFFSSSVHNINVNKIFKFIAAKLFDVPWSVERNLTIGE 305

BLAST of MS008153 vs. TAIR 10
Match: AT5G54840.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.5e-92
Identity = 176/286 (61.54%), Postives = 214/286 (74.83%), Query Frame = 0

Query: 27  AKLTHDAAAAMTKLCRRLV----RWRLLERVSLVRHFFRLIWDSLFVCSS-GRTPGKYRR 86
           A+  H+ A  +T  CR++V    +WR++ERVS+   FFR +W  +  CSS    P  YRR
Sbjct: 2   AEKVHEIAGKITPFCRKIVHVNRKWRIIERVSIFGDFFRFLWRKIVSCSSIIEKPILYRR 61

Query: 87  L-----SPPDAGLSPDDPTTSAGLHSDSDFDS---DDLVPLKISLLGDCQIGKTSFVIKY 146
           +     S        DD TT+    S   F S    DLV LKISLLGDCQ GKT+FV+KY
Sbjct: 62  IVHKISSTAGDIFDGDDDTTTEPTVSGRRFGSSSDSDLVSLKISLLGDCQTGKTTFVVKY 121

Query: 147 VGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGD-RSSQDHLPLACKDAVAILFM 206
           VGDE +   L+MTG+NLM+KT  +Q   ISFSIWDV GD + S+DH+P+ACKDAVAILFM
Sbjct: 122 VGDENQ-SFLEMTGLNLMDKTFYVQGVTISFSIWDVGGDEKRSKDHIPIACKDAVAILFM 181

Query: 207 FDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWTIVSQARAYARVM 266
           FDLTSR TLNSV  WY++ARKWN+TAIPILIGTKFDDFVRLPP+LQWTIV+QARAYA+VM
Sbjct: 182 FDLTSRSTLNSVFGWYSQARKWNKTAIPILIGTKFDDFVRLPPNLQWTIVTQARAYAKVM 241

Query: 267 KATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 299
            A+LFFSS+ HNINVNKIFKFI A+LF++PW I+RNLT+GEPIID+
Sbjct: 242 NASLFFSSATHNINVNKIFKFILARLFNLPWKIDRNLTLGEPIIDY 286

BLAST of MS008153 vs. TAIR 10
Match: AT3G21700.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.3e-75
Identity = 155/296 (52.36%), Postives = 199/296 (67.23%), Query Frame = 0

Query: 37  MTKLCRRLVRW-----RLLERVSLVRHFFRLIWDSLFVCSSGRTPGKYRRL--------S 96
           M + C ++VR      R+  R+ ++R F RL+W  +  C    TPGK RR         S
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVAC----TPGKSRRYLLLSRALPS 60

Query: 97  PPDAGLSPD-----DPTTSAG-------LHSDSDFDSD--------DLVPLKISLLGDCQ 156
           P  +  SP      D     G         S   +D+D        DLV LKISLLGD +
Sbjct: 61  PTVSRPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPE 120

Query: 157 IGKTSFVIKYVGDEQERRSLQM-TGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLPLA 216
           IGKTSF+ KYVG+E+E    ++  GIN  +KTL +  A IS+SIW+++G   S+D +P+A
Sbjct: 121 IGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEGAERSRDQIPVA 180

Query: 217 CKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWTIV 276
           CKD+VAILFMFDLTSR TLNSV+ WY +ARK NQTAIP+++GTKFD+F++LP DLQWTI 
Sbjct: 181 CKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIA 240

Query: 277 SQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 299
           SQAR YA+ + ATLFFSS+ +NINVNKIFKF+ AKLFD+PW++ERNLTIGEPIIDF
Sbjct: 241 SQARTYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVERNLTIGEPIIDF 292

BLAST of MS008153 vs. TAIR 10
Match: AT3G21700.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-74
Identity = 155/296 (52.36%), Postives = 200/296 (67.57%), Query Frame = 0

Query: 37  MTKLCRRLVRW-----RLLERVSLVRHFFRLIWDSLFVCSSGRTPGKYRRL--------S 96
           M + C ++VR      R+  R+ ++R F RL+W  +  C    TPGK RR         S
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVAC----TPGKSRRYLLLSRALPS 60

Query: 97  PPDAGLSPD-----DPTTSAG-------LHSDSDFDSD--------DLVPLKISLLGDCQ 156
           P  +  SP      D     G         S   +D+D        DLV LKISLLGD +
Sbjct: 61  PTVSRPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPE 120

Query: 157 IGKTSFVIKYVGDEQERRSLQM-TGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLPLA 216
           IGKTSF+ KYVG+E+E    ++  GIN  +KTL +  A IS+SIW+++ +R S+D +P+A
Sbjct: 121 IGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAER-SRDQIPVA 180

Query: 217 CKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWTIV 276
           CKD+VAILFMFDLTSR TLNSV+ WY +ARK NQTAIP+++GTKFD+F++LP DLQWTI 
Sbjct: 181 CKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIA 240

Query: 277 SQARAYARVMKATLFFSSSIHNINVNKIFKFIAAKLFDVPWSIERNLTIGEPIIDF 299
           SQAR YA+ + ATLFFSS+ +NINVNKIFKF+ AKLFD+PW++ERNLTIGEPIIDF
Sbjct: 241 SQARTYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVERNLTIGEPIIDF 291

BLAST of MS008153 vs. TAIR 10
Match: AT5G54840.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 195.7 bits (496), Expect = 5.4e-50
Identity = 109/207 (52.66%), Postives = 137/207 (66.18%), Query Frame = 0

Query: 27  AKLTHDAAAAMTKLCRRLV----RWRLLERVSLVRHFFRLIWDSLFVCSS-GRTPGKYRR 86
           A+  H+ A  +T  CR++V    +WR++ERVS+   FFR +W  +  CSS    P  YRR
Sbjct: 2   AEKVHEIAGKITPFCRKIVHVNRKWRIIERVSIFGDFFRFLWRKIVSCSSIIEKPILYRR 61

Query: 87  L-----SPPDAGLSPDDPTTSAGLHSDSDFDS---DDLVPLKISLLGDCQIGKTSFVIKY 146
           +     S        DD TT+    S   F S    DLV LKISLLGDCQ GKT+FV+KY
Sbjct: 62  IVHKISSTAGDIFDGDDDTTTEPTVSGRRFGSSSDSDLVSLKISLLGDCQTGKTTFVVKY 121

Query: 147 VGDEQERRSLQMTGINLMNKTLSIQNALISFSIWDVKGD-RSSQDHLPLACKDAVAILFM 206
           VGDE +   L+MTG+NLM+KT  +Q   ISFSIWDV GD + S+DH+P+ACKDAVAILFM
Sbjct: 122 VGDENQ-SFLEMTGLNLMDKTFYVQGVTISFSIWDVGGDEKRSKDHIPIACKDAVAILFM 181

Query: 207 FDLTSRRTLNSVVDWYTEARKWNQTAI 220
           FDLTSR TLNSV  WY++ARKWN+  +
Sbjct: 182 FDLTSRSTLNSVFGWYSQARKWNKVRL 207

BLAST of MS008153 vs. TAIR 10
Match: AT3G21700.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 189.5 bits (480), Expect = 3.9e-48
Identity = 114/242 (47.11%), Postives = 151/242 (62.40%), Query Frame = 0

Query: 37  MTKLCRRLVRW-----RLLERVSLVRHFFRLIWDSLFVCSSGRTPGKYRRL--------S 96
           M + C ++VR      R+  R+ ++R F RL+W  +  C    TPGK RR         S
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVAC----TPGKSRRYLLLSRALPS 60

Query: 97  PPDAGLSPD-----DPTTSAG-------LHSDSDFDSD--------DLVPLKISLLGDCQ 156
           P  +  SP      D     G         S   +D+D        DLV LKISLLGD +
Sbjct: 61  PTVSRPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPE 120

Query: 157 IGKTSFVIKYVGDEQERRSLQM-TGINLMNKTLSIQNALISFSIWDVKGDRSSQDHLPLA 216
           IGKTSF+ KYVG+E+E    ++  GIN  +KTL +  A IS+SIW+++ +R S+D +P+A
Sbjct: 121 IGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAER-SRDQIPVA 180

Query: 217 CKDAVAILFMFDLTSRRTLNSVVDWYTEARKWNQTAIPILIGTKFDDFVRLPPDLQWTIV 245
           CKD+VAILFMFDLTSR TLNSV+ WY +ARK NQTAIP+++GTKFD+F++LP DLQWTI 
Sbjct: 181 CKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIA 237