MS005006 (gene) Bitter gourd (TR) v1

BLAST of MS005006 vs. NCBI nr
Match: XP_022140340.1 (DNA repair protein RAD16 [Momordica charantia])

HSP 1 Score: 891.3 bits (2302), Expect = 4.5e-255
Identity = 462/676 (68.34%), Postives = 516/676 (76.33%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           EVVETPS LTMPLL YQK WLAWAL++EDS ++GGILADE GMGKTIQA+ALVLAKRE S
Sbjct: 108 EVVETPSALTMPLLRYQKEWLAWALKQEDSSNKGGILADEMGMGKTIQAIALVLAKREFS 167

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
           +  GQ RP  + SS +DLP++K TLVICPV+AVS WVS+IDRFT +GS K+LVYHGPKR 
Sbjct: 168 TSNGQMRPSSHPSSSKDLPEIKTTLVICPVVAVSQWVSEIDRFTSKGSCKVLVYHGPKRV 227

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKER--------------------------- 180
           QS+E+FSEYDFVITTY +VE DY KYLM PK+R                           
Sbjct: 228 QSLETFSEYDFVITTYSVVEADYRKYLMPPKDRCPYCNKQFYQKKLKFHLMYTCGPLSLK 287

Query: 181 -----------PIQPQISKRAEG----------------------------QKPCGELIL 240
                      PIQPQIS + E                             +KPCG+ IL
Sbjct: 288 TEKQAKQQKKKPIQPQISIQEESTKDKINSDHRSDSRKITFEQTVGQNENDKKPCGKSIL 347

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSVKWDRIILDEA F     SN  KA+FAISSSYRWA L G  ++ R   LYSLV FLQ+
Sbjct: 348 HSVKWDRIILDEAHFIKDRLSNTAKAVFAISSSYRWA-LSGTPIQNRIGELYSLVRFLQI 407

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SSLDC  CPHKRVRH+CWWNK+IT  IQN GGTG+D KR M 
Sbjct: 408 VPYSFYFCKDCDCRTLDYSSLDCSNCPHKRVRHFCWWNKYITQRIQNIGGTGQDLKRGMI 467

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRD+LDIQEE FYESL+++S AKF  FV
Sbjct: 468 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDSLDIQEEDFYESLYNDSLAKFNTFV 527

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAEN 480
           AA   TN++AHI D+L+RLRQAVNHPYLVVYSKTSAINCGSSTDN+NEQ+CGICH  AEN
Sbjct: 528 AAGTATNNYAHIFDLLIRLRQAVNHPYLVVYSKTSAINCGSSTDNNNEQVCGICHEPAEN 587

Query: 481 LVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSS 540
            VVTSCEHTFCKAC+IDSTD+F +R+SCP CSK L VDF  TL AGD+ +KTRV+GFKSS
Sbjct: 588 PVVTSCEHTFCKACIIDSTDDFSKRVSCPSCSKMLTVDFSKTLAAGDQPVKTRVKGFKSS 647

Query: 541 SILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISC 600
           SILNRI L+NF TSTKIEALREEI LM ERDGSAKGIV SQFTSFLDLINYSLTKSGI+C
Sbjct: 648 SILNRIQLQNFQTSTKIEALREEIRLMLERDGSAKGIVFSQFTSFLDLINYSLTKSGIAC 707

Query: 601 VQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVER 610
           VQL GS+SLAQ  D I+RFV D DC+I LM+L AGGVA NLTVASHVFIMDPWWNPAVER
Sbjct: 708 VQLIGSMSLAQRADVIKRFVDDSDCKIFLMSLKAGGVALNLTVASHVFIMDPWWNPAVER 767

BLAST of MS005006 vs. NCBI nr
Match: XP_038902046.1 (ATP-dependent helicase rhp16 [Benincasa hispida])

HSP 1 Score: 841.6 bits (2173), Expect = 4.1e-240
Identity = 436/677 (64.40%), Postives = 502/677 (74.15%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  +GGILADE GMGKTIQA+ALVLAKR+LS
Sbjct: 150 EAVETPSALTMPLLRYQKEWLAWALKQEDSSIKGGILADEMGMGKTIQAIALVLAKRQLS 209

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             +G RRP  + SS +DLP +K TLVICPV+AVS WVS+IDRFT +GSYK+LVYHGPKR 
Sbjct: 210 GTSGLRRPSTHPSSSKDLPSIKATLVICPVVAVSQWVSEIDRFTSKGSYKVLVYHGPKRV 269

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E  SEYDFVITTY +VE DY K+LM PK+                            
Sbjct: 270 QSLEILSEYDFVITTYSVVEADYRKHLMPPKDRCPYCNKLFYKKKLKFHLMYICGPDAVK 329

Query: 181 ----------RPIQPQISKRAEG----------------------------QKPCGELIL 240
                     RPIQPQI K+ E                             +KPCG+ +L
Sbjct: 330 TEKQAKQQRKRPIQPQIYKQEESAKGKNNNVHKRGGQKSTLGQTMGQNENDEKPCGKSVL 389

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDR+ILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSL+ FLQ+
Sbjct: 390 HSVIWDRVILDEAHFIKDRLSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLIRFLQI 449

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS  CP CPHKRVRH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 450 VPYSFYFCKDCDCRTLDHSSPTCPNCPHKRVRHFCWWNKNITLRIQNF-GRGPEFKRGMI 509

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSS VL+RTKK +AA+L LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 510 LLKHKILSSTVLRRTKKGRAAELALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 569

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTD-NSNEQLCGICHIAAE 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYSKT+AI+CGS  D ++N QLCGICH  AE
Sbjct: 570 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSKTNAISCGSIADSDNNNQLCGICHEPAE 629

Query: 481 NLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKS 540
             VV+SCEHTFCKAC+ID T++F +R+SCP CSK L +DF T+L   D+T+K  ++GFKS
Sbjct: 630 EPVVSSCEHTFCKACIIDYTNDFSKRVSCPSCSKMLTIDFSTSLAVRDQTVKNTIKGFKS 689

Query: 541 SSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGIS 600
           SSILNRI LENF TSTKIEALREEI  MFERDGSAKGIV SQFTSFLDLINYSLTKSGI+
Sbjct: 690 SSILNRIQLENFQTSTKIEALREEIRFMFERDGSAKGIVFSQFTSFLDLINYSLTKSGIT 749

Query: 601 CVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVE 610
           CVQL GS+SL Q GDAI RF+ DPDC+I LM+L AGG+A NLTVAS+VFIMDPWWNPAVE
Sbjct: 750 CVQLIGSMSLTQRGDAINRFIDDPDCKIFLMSLKAGGIALNLTVASNVFIMDPWWNPAVE 809

BLAST of MS005006 vs. NCBI nr
Match: XP_022943925.1 (DNA repair protein RAD16 [Cucurbita moschata] >XP_022943926.1 DNA repair protein RAD16 [Cucurbita moschata])

HSP 1 Score: 821.2 bits (2120), Expect = 5.8e-234
Identity = 433/678 (63.86%), Postives = 493/678 (72.71%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  RGGILADE GMGKTIQA+ALVLAKRELS
Sbjct: 150 EAVETPSALTMPLLRYQKEWLAWALKQEDSPVRGGILADEMGMGKTIQAIALVLAKRELS 209

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP P  SS +D P +K TLV+CPVIAVS WVS+IDRFT +GS K+ V+HGPKRA
Sbjct: 210 G-AGLRRPSPYPSSSKDFPLIKATLVVCPVIAVSQWVSEIDRFTLKGSNKVHVFHGPKRA 269

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E+  E+DFVITTY +VE +Y K+LM PK+                            
Sbjct: 270 QSLETLFEFDFVITTYSVVEAEYRKHLMPPKDRCPYCSKLFYKKNLKIHLKYICGPDAVK 329

Query: 181 ----------RPIQPQISK----------------------------RAEGQKPCGELIL 240
                     RPIQPQISK                                + PCG+ IL
Sbjct: 330 TEKQAKQIRKRPIQPQISKGEVSAKDKNNNFHNSGSQKSTFGQTMGQHENDENPCGKSIL 389

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDRIILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 390 HSVIWDRIILDEAHFIKDRQSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 449

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS+ CP CPHKR+RH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 450 VPYSFYFCKDCDCRTLDHSSVSCPDCPHKRMRHFCWWNKYITLQIQNV-GRGPEFKRGMI 509

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 510 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 569

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYS+T+ I+CGS   TDN+NEQ CGICH  A
Sbjct: 570 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSRTNVISCGSIDGTDNNNEQACGICHEPA 629

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  VVTSCEHTFCKAC+I   ++F + +SCP CSK L +DF T L   D TIK  ++GFK
Sbjct: 630 EEPVVTSCEHTFCKACIIGFANDFSKLVSCPSCSKMLTIDFSTNLAGRDRTIKNTIKGFK 689

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
            +SILNRI LENF TSTKIEALREEI  M ERDGSAKGIV SQFTSFLDLINYSLTKSGI
Sbjct: 690 CTSILNRIQLENFQTSTKIEALREEIRFMLERDGSAKGIVFSQFTSFLDLINYSLTKSGI 749

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI+RF+ DPDC+I LM+L AGG+A NLTVASHVFIMDPWWNPAV
Sbjct: 750 TCVQLIGSMSLPQRDDAIKRFIDDPDCKIFLMSLKAGGIALNLTVASHVFIMDPWWNPAV 809

BLAST of MS005006 vs. NCBI nr
Match: KAG7010739.1 (RAD16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.8 bits (2119), Expect = 7.5e-234
Identity = 433/678 (63.86%), Postives = 493/678 (72.71%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  RGGILADE GMGKTIQA+ALVLAKRELS
Sbjct: 135 EAVETPSALTMPLLRYQKEWLAWALKQEDSPVRGGILADEMGMGKTIQAIALVLAKRELS 194

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP P  SS +D P +K TLV+CPVIAVS WVS+IDRFT +GS K+ V+HGPKRA
Sbjct: 195 G-AGLRRPSPYPSSSKDFPLIKATLVVCPVIAVSQWVSEIDRFTLKGSNKVHVFHGPKRA 254

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E+  E+DFVITTY +VE +Y K+LM PK+                            
Sbjct: 255 QSLETLFEFDFVITTYSVVEAEYRKHLMPPKDRCPYCSKLFYKKNLKIHLKYICGPDAVK 314

Query: 181 ----------RPIQPQISK----------------------------RAEGQKPCGELIL 240
                     RPIQPQISK                                + PCG+ IL
Sbjct: 315 TEKQAKQIRKRPIQPQISKGEVSAKDKNNNFHNSGSQKSTFGQTMGQHENDENPCGKSIL 374

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDRIILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 375 HSVIWDRIILDEAHFIKDRQSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 434

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS+ CP CPHKR+RH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 435 VPYSFYFCKDCDCRTLDHSSVSCPDCPHKRMRHFCWWNKYITLRIQNV-GRGPEFKRGMI 494

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 495 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 554

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYS+T+ I+CGS   TDN+NEQ CGICH  A
Sbjct: 555 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSRTNVISCGSIDGTDNNNEQACGICHEPA 614

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  VVTSCEHTFCKAC+I   ++F + +SCP CSK L +DF T L   D TIK  ++GFK
Sbjct: 615 EEPVVTSCEHTFCKACIIGFANDFSKLVSCPSCSKMLTIDFSTNLAGRDRTIKNTIKGFK 674

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
            +SILNRI LENF TSTKIEALREEI  M ERDGSAKGIV SQFTSFLDLINYSLTKSGI
Sbjct: 675 CTSILNRIQLENFQTSTKIEALREEIRFMLERDGSAKGIVFSQFTSFLDLINYSLTKSGI 734

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI+RF+ DPDC+I LM+L AGG+A NLTVASHVFIMDPWWNPAV
Sbjct: 735 TCVQLIGSMSLPQRDDAIKRFIDDPDCKIFLMSLKAGGIALNLTVASHVFIMDPWWNPAV 794

BLAST of MS005006 vs. NCBI nr
Match: XP_022986252.1 (DNA repair protein RAD16 [Cucurbita maxima])

HSP 1 Score: 820.8 bits (2119), Expect = 7.5e-234
Identity = 431/678 (63.57%), Postives = 494/678 (72.86%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  RGGILADE GMGKTIQA+ALVLAKRELS
Sbjct: 150 EAVETPSALTMPLLRYQKEWLAWALKQEDSPVRGGILADEMGMGKTIQAIALVLAKRELS 209

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP P  SS +D P +K TLV+CPVIAVS WVS+IDRFT +GS K+ V+HGPKRA
Sbjct: 210 G-AGLRRPSPYPSSSKDFPLIKATLVVCPVIAVSQWVSEIDRFTLKGSNKVHVFHGPKRA 269

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E+  E+DFVITTY +VE +Y K+LM PK+                            
Sbjct: 270 QSLETLFEFDFVITTYSVVEAEYRKHLMPPKDRCPYCSKLFYKKNLKIHLKYICGPDAVK 329

Query: 181 ----------RPIQPQISK----------------------------RAEGQKPCGELIL 240
                     RPIQPQ+SK                                + PCG+ IL
Sbjct: 330 TEKQAKQIRKRPIQPQLSKGEVSAKDKNNNFHNSGSQKSTFGQTMGQHENDENPCGKSIL 389

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDR+ILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 390 HSVIWDRVILDEAHFIKDRQSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 449

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS+ CP CPHKR+RH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 450 VPYSFYFCKDCDCRTLDHSSVSCPDCPHKRMRHFCWWNKYITLRIQNV-GRGPEFKRGMI 509

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 510 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 569

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYS+T+ I+CGS   TDN+NE  CGICH  A
Sbjct: 570 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSRTNVISCGSIDGTDNNNEHACGICHEPA 629

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  VVTSCEHTFCKAC+I   ++F + +SCP CSKKL +DF T L   D+TIK  ++GFK
Sbjct: 630 EEPVVTSCEHTFCKACIIGFANDFSKLVSCPSCSKKLTIDFSTNLAGRDQTIKNTIKGFK 689

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
            +SILNRI LENF TSTKIEALREEI  M ERDGSAKGIV SQFTSFLDLINYSLTKSGI
Sbjct: 690 CTSILNRIQLENFQTSTKIEALREEIRFMLERDGSAKGIVFSQFTSFLDLINYSLTKSGI 749

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI+RF+ DPDC+I LM+L AGG+A NLTVASHVFIMDPWWNPAV
Sbjct: 750 TCVQLIGSMSLPQRDDAIKRFIDDPDCKIFLMSLKAGGIALNLTVASHVFIMDPWWNPAV 809

BLAST of MS005006 vs. ExPASy Swiss-Prot
Match: P79051 (ATP-dependent helicase rhp16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rhp16 PE=3 SV=2)

HSP 1 Score: 384.0 bits (985), Expect = 3.1e-105
Identity = 228/615 (37.07%), Postives = 346/615 (56.26%), Query Frame = 0

Query: 3   VETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELSSI 62
           +E P  L + LL +Q+  + W   +EDS   GGILADE GMGKTIQ +AL+L++      
Sbjct: 245 IEQPKELVLNLLPFQREGVYWLKRQEDSSFGGGILADEMGMGKTIQTIALLLSE------ 304

Query: 63  AGQRRPPNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRAQSI 122
                          P+ K TLV+ PV+A+  W  +ID  T + +    +Y+G  R  S 
Sbjct: 305 ---------------PRGKPTLVVAPVVAIMQWKEEIDTHTNK-ALSTYLYYGQARDISG 364

Query: 123 ESFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVKWDRII 182
           E  S YD V+T+Y ++E  Y K            +   R +      + +LH +++ RII
Sbjct: 365 EELSSYDVVLTSYNVIESVYRK-----------ERSGFRRKNGVVKEKSLLHQMEFYRII 424

Query: 183 LDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYLCK 242
           LDEA        N  +A+  + ++ R   L G  L+ R   L+SL+ FL+  P+++Y C 
Sbjct: 425 LDEAHGIKSRTCNTARAVCGLRTT-RKICLSGTPLQNRIGELFSLLRFLRADPFAYYYCL 484

Query: 243 DCDCRTLDQSSLD---CPKCPHKRVRHYCWWNKHITLPIQNSG--GTGR-DFKRVMRLLK 302
            C+C++L     D   C +C HK + H C++N  +  PIQ  G  G G+  FK+V  LLK
Sbjct: 485 QCECKSLHWRFSDRSNCDECGHKPMSHTCYFNAEMLKPIQKFGYEGPGKLAFKKVHSLLK 544

Query: 303 KDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAE 362
                 I+L+RTK ++A DL LPP +V +R+D  + +EE  Y+SL+ +S  KF  ++A  
Sbjct: 545 -----HIMLRRTKLERADDLGLPPRVVEVRKDLFNEEEEDVYQSLYMDSKRKFNTYLAEG 604

Query: 363 RETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAENLVV 422
              N++A+I  ++ R+RQ  +HP LV+ SK   ++     +N    +C IC   A++ + 
Sbjct: 605 VVLNNYANIFQLITRMRQMADHPDLVLASKRKTVD----IENQENIVCKICDEVAQDAIE 664

Query: 423 TSCEHTFCKACMID--STDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSSS 482
           + C HTFC+ C+ +  +     E ++CP C   L++D                E FK++S
Sbjct: 665 SRCHHTFCRLCVTEYINAAGDGENVNCPSCFIPLSIDLSAPALEDFSE-----EKFKNAS 724

Query: 483 ILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISCV 542
           ILNRI + ++ +STKIEAL EE+ L+ ++D + K IV SQFTS LDLI++ L K+G +CV
Sbjct: 725 ILNRIDMNSWRSSTKIEALVEELYLLRKKDRTLKSIVFSQFTSMLDLIHWRLRKAGFNCV 784

Query: 543 QLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVERQ 602
           +L G ++       IE F +D +  I L++L AGGVA NLT AS VF+MDPWWN AV+ Q
Sbjct: 785 KLDGGMTPKARAATIEAFSNDINITIFLVSLKAGGVALNLTEASQVFMMDPWWNGAVQWQ 811

Query: 603 ALDRVSRIGAYKPIR 610
           A+DR+ RIG  +PI+
Sbjct: 845 AMDRIHRIGQKRPIK 811

BLAST of MS005006 vs. ExPASy Swiss-Prot
Match: P31244 (DNA repair protein RAD16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RAD16 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.5e-99
Identity = 216/616 (35.06%), Postives = 342/616 (55.52%), Query Frame = 0

Query: 6   PSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELSSIAGQ 65
           P  +T+ LL +Q   L W + +E+S++ GG+LADE GMGKTIQ +AL++           
Sbjct: 177 PDGMTIKLLPFQLEGLHWLISQEESIYAGGVLADEMGMGKTIQTIALLM----------- 236

Query: 66  RRPPNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRAQSIESF 125
                     DL K   +LV+ P +A+  W ++I++ T +G  K+ +YHG  R   I+  
Sbjct: 237 ---------NDLTK-SPSLVVAPTVALMQWKNEIEQHT-KGQLKIYIYHGASRTTDIKDL 296

Query: 126 SEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEG--QKPCGELILHSVKWDRIIL 185
             YD V+TTY ++E  + K          Q    +R  G  ++P    +LH++ + R+IL
Sbjct: 297 QGYDVVLTTYAVLESVFRK----------QNYGFRRKNGLFKQPS---VLHNIDFYRVIL 356

Query: 186 DEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYLCKD 245
           DEA       SN  +A+  + +  RW  L G  L+ R   +YSL+ FL ++P++ Y C  
Sbjct: 357 DEAHNIKDRQSNTARAVNNLKTQKRWC-LSGTPLQNRIGEMYSLIRFLNINPFTKYFCTK 416

Query: 246 CDCRTLDQSSLD---CPKCPHKRVRHYCWWNKHITLPIQNSGGTG---RDFKRVMRLLKK 305
           CDC + D    D   C  C H  ++H  ++N  +   IQ  G  G     F  +  LLK 
Sbjct: 417 CDCASKDWKFTDRMHCDHCSHVIMQHTNFFNHFMLKNIQKFGVEGPGLESFNNIQTLLK- 476

Query: 306 DILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAER 365
               +I+L+RTK ++A DL LPP IV++RRD  + +E+  Y SL+ +S  K+ +FV    
Sbjct: 477 ----NIMLRRTKVERADDLGLPPRIVTVRRDFFNEEEKDLYRSLYTDSKRKYNSFVEEGV 536

Query: 366 ETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAENLVVT 425
             N++A+I  ++ R+RQ  +HP LV+      +N     D+    +C +C+  AE  + +
Sbjct: 537 VLNNYANIFTLITRMRQLADHPDLVL----KRLN-NFPGDDIGVVICQLCNDEAEEPIES 596

Query: 426 SCEHTFCKACMIDSTDNFWE---RISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSSS 485
            C H FC+ C+ +  ++F E   +++CPVC   L++D           ++  ++ FK  S
Sbjct: 597 KCHHKFCRLCIKEYVESFMENNNKLTCPVCHIGLSIDL------SQPALEVDLDSFKKQS 656

Query: 486 ILNRIPLE-NFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISC 545
           I++R+ +   + +STKIEAL EE+  +     + K IV SQFTS LDL+ + L ++G   
Sbjct: 657 IVSRLNMSGKWQSSTKIEALVEELYKLRSNKRTIKSIVFSQFTSMLDLVEWRLKRAGFQT 716

Query: 546 VQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVER 605
           V+L GS+S  Q  + I+ F+++  C + L++L AGGVA NL  AS VFI+DPWWNP+VE 
Sbjct: 717 VKLQGSMSPTQRDETIKYFMNNIQCEVFLVSLKAGGVALNLCEASQVFILDPWWNPSVEW 740

Query: 606 QALDRVSRIGAYKPIR 610
           Q+ DRV RIG Y+P++
Sbjct: 777 QSGDRVHRIGQYRPVK 740

BLAST of MS005006 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.6e-69
Identity = 207/705 (29.36%), Postives = 307/705 (43.55%), Query Frame = 0

Query: 1    EVVETPSTLTMPLLGYQKAWLAWALEKE---------DSLH------------------- 60
            E +E PSTLT  L  YQK  L W  E E         ++LH                   
Sbjct: 604  EEMEAPSTLTCNLRPYQKQALYWMSESEKGIDVEKAAETLHPCWEAYRICDERAPSIYLN 663

Query: 61   -----------------RGGILADEKGMGKTIQALALVLAKRELSSIAGQRRPPN----- 120
                             RGGILAD  G+GKT+  +AL+LA+       G+  P N     
Sbjct: 664  IFSGEATIQFPTATQMARGGILADAMGLGKTVMTIALILAR------PGRGNPENEDVLV 723

Query: 121  ASSREDLPKMK-------------GTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPK 180
            A    D    K             GTL+ICP+  +S W  +++  ++  +  +LVY+G  
Sbjct: 724  ADVNADKRNRKEIHMALTTVKAKGGTLIICPMALLSQWKDELETHSKPDTVSVLVYYGGD 783

Query: 181  RAQSIESFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVK 240
            R    ++ + +D V+TTY ++   Y                      ++     I H + 
Sbjct: 784  RTHDAKAIASHDVVLTTYGVLTSAY----------------------KQDMANSIFHRID 843

Query: 241  WDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYS 300
            W RI+LDEA       +   KA F +SS  RW L   P L+ +   LYSL+CFL V P+ 
Sbjct: 844  WYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTP-LQNKLEDLYSLLCFLHVEPWC 903

Query: 301  FYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKK 360
                                        ++ WW+K I  P +N  G  R  K     L K
Sbjct: 904  ----------------------------NWAWWSKLIQKPYEN--GDPRGLK-----LIK 963

Query: 361  DILSSIVLQRTK--KDKAADLV--LPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
             IL  ++L+RTK  +DK   L+  LPP+ V +        E  FY +L   S  +F  FV
Sbjct: 964  AILRPLMLRRTKETRDKEGSLILELPPTDVQVIECEQSEAERDFYTALFKRSKVQFDQFV 1023

Query: 421  AAERETNDFAHIDDILVRLRQAVNHPYLVV-------YSKTSAI-------NCGSSTDNS 480
            A  +  +++A+I ++L+RLRQ  NHP+LV+       Y+   ++       N  S + N+
Sbjct: 1024 AQGKVLHNYANILELLLRLRQCCNHPFLVMSRADSQQYADLDSLARRFLDNNPDSVSQNA 1083

Query: 481  ----------------NEQLCGICHIAAENLVVTSCEHTFCKACMIDSTDNFWERISCPV 540
                            N + C IC  +A++ V+T C H  C+ C++ S    W   SC +
Sbjct: 1084 PSRAYIEEVIQDLRDGNSKECPICLESADDPVLTPCAHRMCRECLLTS----WRSPSCGL 1143

Query: 541  CSKKLAVDFCTTLTAGDETIKTRVEGFKSSSILNRIPLENFLTSTKIEALREEIILMFER 600
            C        C T+    E I        + SI     ++N+  S+K+  L + +  + + 
Sbjct: 1144 C------PICRTILKRTELISC-----PTDSIFRVDVVKNWKESSKVSELLKCLEKIKKS 1203

Query: 601  DGSAKGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQGGDAIERFVHDPDCRILLM 609
                K IV SQ+TSFLDL+   L + G   ++  G ++       ++ F       ILLM
Sbjct: 1204 GSGEKSIVFSQWTSFLDLLEIPLRRRGFEFLRFDGKLAQKGREKVLKEFNETKQKTILLM 1229

BLAST of MS005006 vs. ExPASy Swiss-Prot
Match: Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.2e-64
Identity = 191/643 (29.70%), Postives = 299/643 (46.50%), Query Frame = 0

Query: 33  RGGILADEKGMGKTIQALALVLAKRELSSIAGQRRPPN------ASSREDL--PKMK--- 92
           RGGILAD  G+GKT+  ++L+LA    ++  G   P        +SS +DL  P +K   
Sbjct: 413 RGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISSSVDDLTSPPVKATK 472

Query: 93  ------------------GTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRAQSIE 152
                             G L++CP+  +  W ++I+   + GS  + V++G  R +  +
Sbjct: 473 FLGFDKRLLEQKSVLQNGGNLIVCPMTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAK 532

Query: 153 SFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVKWDRIIL 212
             S+ D VITTY ++  ++ +            + S   EG        +++V+W RI+L
Sbjct: 533 LLSQSDVVITTYGVLTSEFSQ------------ENSADHEG--------IYAVRWFRIVL 592

Query: 213 DEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYLCKD 272
           DEA       S I+ A  A+ +  RW L   P ++     LYSL+ FL++ P+       
Sbjct: 593 DEAHTIKNSKSQISLAAAALVADRRWCLTGTP-IQNNLEDLYSLLRFLRIEPWG------ 652

Query: 273 CDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKKDILSSI 332
                                  + WWNK +  P +   G  R  K V  +LK      I
Sbjct: 653 ----------------------TWAWWNKLVQKPFEE--GDERGLKLVQSILK-----PI 712

Query: 333 VLQRTKKDKAAD----LVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAERET 392
           +L+RTK     +    LVLPP+   +    L   E  FY++L   S  KF  FV   +  
Sbjct: 713 MLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYDALFKRSKVKFDQFVEQGKVL 772

Query: 393 NDFAHIDDILVRLRQAVNHPYLVV-------YSKTSAINCGSSTDNSN------------ 452
           +++A I ++L+RLRQ  +HP+LV+       YS  + ++    +  S+            
Sbjct: 773 HNYASILELLLRLRQCCDHPFLVMSRGDTAEYSDLNKLSKRFLSGKSSGLEREGKDVPSE 832

Query: 453 -------EQL-------CGICHIAAENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSK 512
                  E+L       C IC  A E+ V+T C H  C+ C++ S  N    + CPVC  
Sbjct: 833 AFVQEVVEELRKGEQGECPICLEALEDAVLTPCAHRLCRECLLASWRNSTSGL-CPVCRN 892

Query: 513 KLAVDFCTTLTAGDETIKTRVEGFKSSSILNRIPLENFLTSTKIEALREEIILMFERDGS 572
            ++     T    +   +  VE             +N++ S+KI AL EE+  +  R   
Sbjct: 893 TVSKQELITAPT-ESRFQVDVE-------------KNWVESSKITALLEELEGL--RSSG 952

Query: 573 AKGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLN 610
           +K I+ SQ+T+FLDL+   L+++  S V+L G++S  Q    ++ F  D    +LLM+L 
Sbjct: 953 SKSILFSQWTAFLDLLQIPLSRNNFSFVRLDGTLSQQQREKVLKEFSEDGSILVLLMSLK 982

BLAST of MS005006 vs. ExPASy Swiss-Prot
Match: O60177 (Uncharacterized ATP-dependent helicase C23E6.02 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC23E6.02 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 6.5e-63
Identity = 188/663 (28.36%), Postives = 297/663 (44.80%), Query Frame = 0

Query: 5   TPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELSSIAG 64
           TP+ L   L+ +QK  L W    E+S  +GGILAD+ G+GKT+QALAL++          
Sbjct: 382 TPAGLIPTLMEHQKEGLMWLKRLEESSKKGGILADDMGLGKTVQALALLVT--------- 441

Query: 65  QRRPPNASSREDLPKMKGTLVICPVIAVSVWVSQI-DRFTRRGSYKLLVYHGPKRAQSI- 124
             RPP + S      +K TL+I PV  +  W ++I  +        + ++HG  +   I 
Sbjct: 442 --RPPESKS------VKTTLIITPVSLLQQWHNEILTKIAPSHRPTVYIHHGSSKKHKIA 501

Query: 125 ESFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVKWDRII 184
           E    YD V+TTY ++  ++   +   K       I K          L     +W R+I
Sbjct: 502 EQLMSYDIVLTTYNVIAYEFKNKMAYDKSIEDNAPIKK-------FEHLPFFEAEWYRVI 561

Query: 185 LDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYLCK 244
           LDEA+      +   +    + S+YRW  L G  ++      YSL+ FL++ PYS     
Sbjct: 562 LDEAQTIKNRNTLAARGCCLLESTYRWC-LSGTPMQNGVEEFYSLIKFLRIKPYS----- 621

Query: 245 DCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKKDILSS 304
                  D SS                ++K  T+P+ ++  T    KR   LLK     +
Sbjct: 622 -------DWSS----------------FSKDFTIPLSSNINTSAPMKRFRGLLK-----A 681

Query: 305 IVLQRTKKDKAAD---LVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAERET 364
           ++L+RTK  K      L LPP         L   E  FY +L   +  + R ++     T
Sbjct: 682 VLLRRTKNTKIDGKPILTLPPKTAVKSETDLSSSEMEFYNTLQSGAQIQMRKYLQEGTIT 741

Query: 365 NDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQL---------------- 424
             +  +  +L+RLRQA  HP+L+V ++ +A++   S    N  +                
Sbjct: 742 THYGSLLVLLLRLRQACCHPWLIV-AREAAVDDNDSFQAKNRAIYNQIYPEAVNRLKLIE 801

Query: 425 ---CGIC-HIAAENLVVTSCEHTFCKACM---IDSTDNFWERIS-------CPVCSKKLA 484
              C +C  + AE L++  C H  C+ C+   I S+++  ++ S       C VC + + 
Sbjct: 802 TLQCSLCMDVVAELLIIVPCGHFLCRECLTHVITSSEDMAKQTSNENISPKCSVCEEYID 861

Query: 485 VD----------FCTTLTAGDETIKTRVEGFKS------SSILNRIPL--------ENFL 544
            +          +       D   K R E          S+IL    +        +++ 
Sbjct: 862 TERLLSYALFRRYSGMAPIVDADNKLRTENISELLPKQYSNILENRQMGMKIFTDPKHWT 921

Query: 545 TSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQG 604
           TSTKIE     +  + ++  + K ++ SQF SFL+L      + GI  +  TG +S A+ 
Sbjct: 922 TSTKIEKALNAVKEIIKKQPTDKILIFSQFVSFLELFTVPFRQEGIKYLMYTGGLSTAER 981

Query: 605 GDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVERQALDRVSRIGAY 609
             A+  F  DP+ R+LL++L AG V  NLT A+HV I+DP+WNP +E QA+DR  RIG  
Sbjct: 982 NQALINFEVDPNVRVLLISLKAGNVGLNLTCANHVIILDPFWNPYIEEQAVDRAHRIGQD 985

BLAST of MS005006 vs. ExPASy TrEMBL
Match: A0A6J1CET8 (DNA repair protein RAD16 OS=Momordica charantia OX=3673 GN=LOC111011036 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 2.2e-255
Identity = 462/676 (68.34%), Postives = 516/676 (76.33%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           EVVETPS LTMPLL YQK WLAWAL++EDS ++GGILADE GMGKTIQA+ALVLAKRE S
Sbjct: 108 EVVETPSALTMPLLRYQKEWLAWALKQEDSSNKGGILADEMGMGKTIQAIALVLAKREFS 167

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
           +  GQ RP  + SS +DLP++K TLVICPV+AVS WVS+IDRFT +GS K+LVYHGPKR 
Sbjct: 168 TSNGQMRPSSHPSSSKDLPEIKTTLVICPVVAVSQWVSEIDRFTSKGSCKVLVYHGPKRV 227

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKER--------------------------- 180
           QS+E+FSEYDFVITTY +VE DY KYLM PK+R                           
Sbjct: 228 QSLETFSEYDFVITTYSVVEADYRKYLMPPKDRCPYCNKQFYQKKLKFHLMYTCGPLSLK 287

Query: 181 -----------PIQPQISKRAEG----------------------------QKPCGELIL 240
                      PIQPQIS + E                             +KPCG+ IL
Sbjct: 288 TEKQAKQQKKKPIQPQISIQEESTKDKINSDHRSDSRKITFEQTVGQNENDKKPCGKSIL 347

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSVKWDRIILDEA F     SN  KA+FAISSSYRWA L G  ++ R   LYSLV FLQ+
Sbjct: 348 HSVKWDRIILDEAHFIKDRLSNTAKAVFAISSSYRWA-LSGTPIQNRIGELYSLVRFLQI 407

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SSLDC  CPHKRVRH+CWWNK+IT  IQN GGTG+D KR M 
Sbjct: 408 VPYSFYFCKDCDCRTLDYSSLDCSNCPHKRVRHFCWWNKYITQRIQNIGGTGQDLKRGMI 467

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRD+LDIQEE FYESL+++S AKF  FV
Sbjct: 468 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDSLDIQEEDFYESLYNDSLAKFNTFV 527

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAEN 480
           AA   TN++AHI D+L+RLRQAVNHPYLVVYSKTSAINCGSSTDN+NEQ+CGICH  AEN
Sbjct: 528 AAGTATNNYAHIFDLLIRLRQAVNHPYLVVYSKTSAINCGSSTDNNNEQVCGICHEPAEN 587

Query: 481 LVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSS 540
            VVTSCEHTFCKAC+IDSTD+F +R+SCP CSK L VDF  TL AGD+ +KTRV+GFKSS
Sbjct: 588 PVVTSCEHTFCKACIIDSTDDFSKRVSCPSCSKMLTVDFSKTLAAGDQPVKTRVKGFKSS 647

Query: 541 SILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISC 600
           SILNRI L+NF TSTKIEALREEI LM ERDGSAKGIV SQFTSFLDLINYSLTKSGI+C
Sbjct: 648 SILNRIQLQNFQTSTKIEALREEIRLMLERDGSAKGIVFSQFTSFLDLINYSLTKSGIAC 707

Query: 601 VQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVER 610
           VQL GS+SLAQ  D I+RFV D DC+I LM+L AGGVA NLTVASHVFIMDPWWNPAVER
Sbjct: 708 VQLIGSMSLAQRADVIKRFVDDSDCKIFLMSLKAGGVALNLTVASHVFIMDPWWNPAVER 767

BLAST of MS005006 vs. ExPASy TrEMBL
Match: A0A6J1FYD3 (DNA repair protein RAD16 OS=Cucurbita moschata OX=3662 GN=LOC111448500 PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.8e-234
Identity = 433/678 (63.86%), Postives = 493/678 (72.71%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  RGGILADE GMGKTIQA+ALVLAKRELS
Sbjct: 150 EAVETPSALTMPLLRYQKEWLAWALKQEDSPVRGGILADEMGMGKTIQAIALVLAKRELS 209

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP P  SS +D P +K TLV+CPVIAVS WVS+IDRFT +GS K+ V+HGPKRA
Sbjct: 210 G-AGLRRPSPYPSSSKDFPLIKATLVVCPVIAVSQWVSEIDRFTLKGSNKVHVFHGPKRA 269

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E+  E+DFVITTY +VE +Y K+LM PK+                            
Sbjct: 270 QSLETLFEFDFVITTYSVVEAEYRKHLMPPKDRCPYCSKLFYKKNLKIHLKYICGPDAVK 329

Query: 181 ----------RPIQPQISK----------------------------RAEGQKPCGELIL 240
                     RPIQPQISK                                + PCG+ IL
Sbjct: 330 TEKQAKQIRKRPIQPQISKGEVSAKDKNNNFHNSGSQKSTFGQTMGQHENDENPCGKSIL 389

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDRIILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 390 HSVIWDRIILDEAHFIKDRQSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 449

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS+ CP CPHKR+RH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 450 VPYSFYFCKDCDCRTLDHSSVSCPDCPHKRMRHFCWWNKYITLQIQNV-GRGPEFKRGMI 509

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 510 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 569

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYS+T+ I+CGS   TDN+NEQ CGICH  A
Sbjct: 570 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSRTNVISCGSIDGTDNNNEQACGICHEPA 629

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  VVTSCEHTFCKAC+I   ++F + +SCP CSK L +DF T L   D TIK  ++GFK
Sbjct: 630 EEPVVTSCEHTFCKACIIGFANDFSKLVSCPSCSKMLTIDFSTNLAGRDRTIKNTIKGFK 689

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
            +SILNRI LENF TSTKIEALREEI  M ERDGSAKGIV SQFTSFLDLINYSLTKSGI
Sbjct: 690 CTSILNRIQLENFQTSTKIEALREEIRFMLERDGSAKGIVFSQFTSFLDLINYSLTKSGI 749

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI+RF+ DPDC+I LM+L AGG+A NLTVASHVFIMDPWWNPAV
Sbjct: 750 TCVQLIGSMSLPQRDDAIKRFIDDPDCKIFLMSLKAGGIALNLTVASHVFIMDPWWNPAV 809

BLAST of MS005006 vs. ExPASy TrEMBL
Match: A0A6J1J723 (DNA repair protein RAD16 OS=Cucurbita maxima OX=3661 GN=LOC111484062 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.6e-234
Identity = 431/678 (63.57%), Postives = 494/678 (72.86%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETPS LTMPLL YQK WLAWAL++EDS  RGGILADE GMGKTIQA+ALVLAKRELS
Sbjct: 150 EAVETPSALTMPLLRYQKEWLAWALKQEDSPVRGGILADEMGMGKTIQAIALVLAKRELS 209

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP P  SS +D P +K TLV+CPVIAVS WVS+IDRFT +GS K+ V+HGPKRA
Sbjct: 210 G-AGLRRPSPYPSSSKDFPLIKATLVVCPVIAVSQWVSEIDRFTLKGSNKVHVFHGPKRA 269

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           QS+E+  E+DFVITTY +VE +Y K+LM PK+                            
Sbjct: 270 QSLETLFEFDFVITTYSVVEAEYRKHLMPPKDRCPYCSKLFYKKNLKIHLKYICGPDAVK 329

Query: 181 ----------RPIQPQISK----------------------------RAEGQKPCGELIL 240
                     RPIQPQ+SK                                + PCG+ IL
Sbjct: 330 TEKQAKQIRKRPIQPQLSKGEVSAKDKNNNFHNSGSQKSTFGQTMGQHENDENPCGKSIL 389

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDR+ILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 390 HSVIWDRVILDEAHFIKDRQSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 449

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS+ CP CPHKR+RH+CWWNK+ITL IQN  G G +FKR M 
Sbjct: 450 VPYSFYFCKDCDCRTLDHSSVSCPDCPHKRMRHFCWWNKYITLRIQNV-GRGPEFKRGMI 509

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPSIVSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 510 LLKHKILSSIVLRRTKKGRAADLALPPSIVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 569

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYS+T+ I+CGS   TDN+NE  CGICH  A
Sbjct: 570 AAGTATSNYAHIFDLLIRLRQAVNHPYLVVYSRTNVISCGSIDGTDNNNEHACGICHEPA 629

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  VVTSCEHTFCKAC+I   ++F + +SCP CSKKL +DF T L   D+TIK  ++GFK
Sbjct: 630 EEPVVTSCEHTFCKACIIGFANDFSKLVSCPSCSKKLTIDFSTNLAGRDQTIKNTIKGFK 689

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
            +SILNRI LENF TSTKIEALREEI  M ERDGSAKGIV SQFTSFLDLINYSLTKSGI
Sbjct: 690 CTSILNRIQLENFQTSTKIEALREEIRFMLERDGSAKGIVFSQFTSFLDLINYSLTKSGI 749

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI+RF+ DPDC+I LM+L AGG+A NLTVASHVFIMDPWWNPAV
Sbjct: 750 TCVQLIGSMSLPQRDDAIKRFIDDPDCKIFLMSLKAGGIALNLTVASHVFIMDPWWNPAV 809

BLAST of MS005006 vs. ExPASy TrEMBL
Match: A0A0A0LN53 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G416820 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 5.3e-233
Identity = 430/678 (63.42%), Postives = 495/678 (73.01%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETP+ LTMPLL YQK WLAWAL++EDS  +GGILADE GMGKTIQA+ALVLAKR+LS
Sbjct: 151 EAVETPAALTMPLLRYQKEWLAWALKQEDSSIKGGILADEMGMGKTIQAIALVLAKRQLS 210

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP  N SS +DLP +K TLVICPV+AVS WVS+IDRFT  GSYK+LVYHGPKR 
Sbjct: 211 GTAGLRRPSSNPSSSKDLPLIKATLVICPVVAVSQWVSEIDRFTSEGSYKVLVYHGPKRE 270

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           +S+E  SEYDFVITTY +VE DY KYLM PK+                            
Sbjct: 271 RSLEVLSEYDFVITTYSVVEADYRKYLMPPKDRCPYCSKLFHKKNLKFHLMYICGPDAVK 330

Query: 181 ----------RPIQPQISK-------------RAEGQKPC---------------GELIL 240
                     RPIQPQI K             ++ GQK                 G  IL
Sbjct: 331 TEKQSKQQRKRPIQPQICKQEKSDKDKNNNVHKSGGQKSTLGQTVEEHENDEKHRGNSIL 390

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDR+ILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 391 HSVIWDRVILDEAHFIKDRLSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 450

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SSL CP CPHKRVRH+CWWNK+I+  IQN  G G +FKR M 
Sbjct: 451 VPYSFYFCKDCDCRTLDHSSLTCPNCPHKRVRHFCWWNKNISQRIQNF-GRGPEFKRGMI 510

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILS+IVL+RTKK +AADL LPPS VSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 511 LLKHKILSTIVLRRTKKGRAADLALPPSTVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 570

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYSKT+AIN G+   +D++N+Q+CGIC+  A
Sbjct: 571 AAGTVTSNYAHIFDLLIRLRQAVNHPYLVVYSKTNAINSGNIDDSDSNNKQVCGICYEPA 630

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  V TSC+HTFCKAC+ID   +F + +SCP CSK L  DF T++   D+T+K +++GFK
Sbjct: 631 EEPVDTSCKHTFCKACLIDYAGDFSKPVSCPSCSKMLTSDFITSMAFKDQTVKNKIKGFK 690

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
           SSSILNRI LENF TSTKIEALREEI  MFERDGSAKGIV SQFTSFLDLINYSL+KSGI
Sbjct: 691 SSSILNRIQLENFQTSTKIEALREEIRFMFERDGSAKGIVFSQFTSFLDLINYSLSKSGI 750

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI RF+ DPDC+I LM+L AGGVA NLTVASHVFIMDPWWNPAV
Sbjct: 751 TCVQLVGSMSLTQRADAINRFIEDPDCKIFLMSLKAGGVALNLTVASHVFIMDPWWNPAV 810

BLAST of MS005006 vs. ExPASy TrEMBL
Match: A0A5D3DZG8 (DNA repair protein RAD16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00010 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 1.2e-232
Identity = 428/678 (63.13%), Postives = 491/678 (72.42%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E VETP+ LTMPLL YQK WLAWAL++EDS  +GGILADE GMGKTIQA+ALVLAKR+LS
Sbjct: 146 ESVETPAALTMPLLRYQKEWLAWALKQEDSSIKGGILADEMGMGKTIQAIALVLAKRQLS 205

Query: 61  SIAGQRRP-PNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
             AG RRP  N SS ++LP +K TLVICPV+AVS WVS+IDRFT  GSYK+LVYHGPKR 
Sbjct: 206 GTAGLRRPSSNPSSSKELPLIKATLVICPVVAVSQWVSEIDRFTSEGSYKVLVYHGPKRV 265

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLMLPKE---------------------------- 180
           +S+E  SEYDFVITTY +VE DY KYLM PK+                            
Sbjct: 266 RSLEILSEYDFVITTYSVVEADYRKYLMPPKDRCPYCSKLFHKKNLKFHLMYICGPDAVK 325

Query: 181 ----------RPIQPQISKRAEG----------------------------QKPCGELIL 240
                     RPIQPQI K+ +                             +KP G  IL
Sbjct: 326 TEKQSKQQRKRPIQPQICKQEKSDKDKNNNVHKSGAQKSTLGQTLGEHENDEKPRGNSIL 385

Query: 241 HSVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQV 300
           HSV WDR+ILDEA F     SN  KA+ AISSS+RWA L G  ++ R   LYSLV FLQ+
Sbjct: 386 HSVIWDRVILDEAHFIKDRLSNTAKAVLAISSSFRWA-LSGTPIQNRVGELYSLVRFLQI 445

Query: 301 HPYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMR 360
            PYSFY CKDCDCRTLD SS  CP CPHKRVRH+CWWNK+IT  IQN  G G +FKR M 
Sbjct: 446 VPYSFYFCKDCDCRTLDHSSPTCPNCPHKRVRHFCWWNKNITQRIQNF-GRGPEFKRGMI 505

Query: 361 LLKKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
           LLK  ILSSIVL+RTKK +AADL LPPS VSIRRDTLDIQEE FYESL+++S AKF  FV
Sbjct: 506 LLKHKILSSIVLRRTKKGRAADLALPPSTVSIRRDTLDIQEEDFYESLYNDSRAKFNTFV 565

Query: 421 AAERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGS--STDNSNEQLCGICHIAA 480
           AA   T+++AHI D+L+RLRQAVNHPYLVVYSKT AIN G+   +D++N+Q+CG+CH  A
Sbjct: 566 AAGTVTSNYAHIFDLLIRLRQAVNHPYLVVYSKTKAINSGNIDDSDSNNKQVCGLCHEPA 625

Query: 481 ENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFK 540
           E  V TSC+H FCKAC+ID   +F + +SCP CSK L  DF T++   D+T+K  ++GFK
Sbjct: 626 EEPVDTSCKHAFCKACIIDYAGDFSKPVSCPSCSKMLTSDFITSMAFKDQTVKNTIKGFK 685

Query: 541 SSSILNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGI 600
           SSSILNRI LENF TSTKIEALREEI  MFERDGSAKGIV SQFTSFLDLINYSL+KSGI
Sbjct: 686 SSSILNRIQLENFQTSTKIEALREEIRFMFERDGSAKGIVFSQFTSFLDLINYSLSKSGI 745

Query: 601 SCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAV 610
           +CVQL GS+SL Q  DAI RF+ DPDC+I LM+L AGGVA NLTVASHVFIMDPWWNPAV
Sbjct: 746 TCVQLVGSMSLTQRADAINRFIEDPDCKIFLMSLKAGGVALNLTVASHVFIMDPWWNPAV 805

BLAST of MS005006 vs. TAIR 10
Match: AT1G05120.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 632.5 bits (1630), Expect = 3.5e-181
Identity = 343/674 (50.89%), Postives = 431/674 (63.95%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E  E PS L MPLL YQK +LAWA ++E S+  GGILADE GMGKTIQA++LVLA+RE+ 
Sbjct: 127 ETAEPPSDLIMPLLRYQKEFLAWATKQEQSV-AGGILADEMGMGKTIQAISLVLARREV- 186

Query: 61  SIAGQRRPPNASSREDLPKMKG-TLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRA 120
                        R    +  G TLV+CP++AVS W+++I RFT  GS K+LVYHG KRA
Sbjct: 187 ------------DRAQFGEAAGCTLVLCPLVAVSQWLNEIARFTSPGSTKVLVYHGAKRA 246

Query: 121 QSIESFSEYDFVITTYFIVEDDYMKYLM-------------LPKERPIQ------PQISK 180
           ++I+ F  YDFV+TTY  VE +Y + +M              PK+  I       P   K
Sbjct: 247 KNIKEFMNYDFVLTTYSTVESEYRRNIMPSKVQCAYCSKSFYPKKLVIHLRYFCGPSAVK 306

Query: 181 RAEGQK-----------------PCGE---------------------------LILHSV 240
            A+  K                   GE                            +LHSV
Sbjct: 307 TAKQSKQKRKKTSDSSSQQGKEADAGEDKKLKKSKKKTKQTVEKDQLGSDDKEKSLLHSV 366

Query: 241 KWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPY 300
           KW+RIILDEA +     SN  +A+FA+ ++YRWA L G  L+ R   LYSL+ FLQ+ PY
Sbjct: 367 KWNRIILDEAHYIKERRSNTARAVFALEATYRWA-LSGTPLQNRVGELYSLIRFLQIRPY 426

Query: 301 SFYLCKDCDCRTLDQ-SSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLL 360
           S+Y CKDCDCR LD  +   CP CPH  VRH+CWWNK++  PI   G  G   KR M LL
Sbjct: 427 SYYFCKDCDCRILDYVAHQSCPHCPHNAVRHFCWWNKYVAKPITVYGSFGLG-KRAMILL 486

Query: 361 KKDILSSIVLQRTKKDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAA 420
           K  +L  I+L+RTK  +AADL LPP I+++RRDTLD++E  +YESL+  S A+F  ++ A
Sbjct: 487 KHKVLKDILLRRTKLGRAADLALPPRIITLRRDTLDVKEFDYYESLYKNSQAEFNTYIEA 546

Query: 421 ERETNDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAENLV 480
               N++AHI D+L RLRQAV+HPYLVVYS +S  N     +N +EQ CG+CH  AE+ V
Sbjct: 547 GTLMNNYAHIFDLLTRLRQAVDHPYLVVYSNSSGANANLVDENKSEQECGLCHDPAEDYV 606

Query: 481 VTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSSSI 540
           VTSC H FCKAC+I  + +   +++CP CSK L VD+ T      +  KT ++GF++SSI
Sbjct: 607 VTSCAHVFCKACLIGFSASL-GKVTCPTCSKLLTVDWTTKADTEHKASKTTLKGFRASSI 666

Query: 541 LNRIPLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISCVQ 600
           LNRI L++F TSTKIEALREEI  M ERDGSAK IV SQFTSFLDLINY+L K G+SCVQ
Sbjct: 667 LNRIKLDDFQTSTKIEALREEIRFMVERDGSAKAIVFSQFTSFLDLINYTLGKCGVSCVQ 726

Query: 601 LTGSISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVERQA 610
           L GS+++A    AI +F  DPDCR+ LM+L AGGVA NLTVASHVF+MDPWWNPAVERQA
Sbjct: 727 LVGSMTMAARDTAINKFKEDPDCRVFLMSLKAGGVALNLTVASHVFMMDPWWNPAVERQA 783

BLAST of MS005006 vs. TAIR 10
Match: AT1G02670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 9.8e-115
Identity = 260/610 (42.62%), Postives = 339/610 (55.57%), Query Frame = 0

Query: 1   EVVETPSTLTMPLLGYQKAWLAWALEKEDSLHRGGILADEKGMGKTIQALALVLAKRELS 60
           E  E P  L +PLL YQK +LAWA  +E S  RGGILADE GMGKTIQA++LVLA+RE+ 
Sbjct: 122 EAAEQPLDLIIPLLKYQKEFLAWATIQELSAVRGGILADEMGMGKTIQAISLVLARREVD 181

Query: 61  SIAGQRRPPNASSREDLPKMKGTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRAQ 120
                     A SRE    +  TLV+ P +A+S W+ +I R T  GS ++L YHGPKR +
Sbjct: 182 ---------RAKSRE---AVGHTLVLVPPVALSQWLDEISRLTSPGSTRVLQYHGPKRDK 241

Query: 121 SIESFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVKWDR 180
           +++    YDFV+TT  IVE++Y       K+  +   +S             LHS+KW+R
Sbjct: 242 NVQKLMNYDFVLTTSPIVENEYR------KDEGVDETMSP------------LHSIKWNR 301

Query: 181 IILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYL 240
           II+DEA       S   KA+FA+ ++YRWA L G  L+     LYSLV       YSF  
Sbjct: 302 IIVDEAHDIKNRSSRTAKAVFALEATYRWA-LSGTPLQNDVDELYSLV------SYSFL- 361

Query: 241 CKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKKDIL 300
                               +     + + + HIT     +         V  L+  +IL
Sbjct: 362 -----------------NFFYSTYASFAFRHTHITFARNVT---------VKFLIGGNIL 421

Query: 301 SSIVLQRTKKDKAADLV-LPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAERET 360
              +  R +   A  ++ +  S+   RRD L + E  FYESL+  S   F  ++ A    
Sbjct: 422 PLSIPVRIENVPAVLIMQINTSLGGKRRDALSVVEADFYESLYKVSKTTFDGYIQAGTLM 481

Query: 361 NDFAHIDDILVRLRQAVNHPYLVVYSKTSAINCGSSTDNSNEQLCGICHIAAENLVVTSC 420
           N++AHI  +L+RLRQAV+HPYLV YS  S  N      N NE+ CG  H  +++  VTS 
Sbjct: 482 NNYAHIFGLLIRLRQAVDHPYLVSYSSPSGANANLLDANKNEKECGFGHDPSKDYFVTSS 541

Query: 421 EHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKTRVEGFKSSSILNRI 480
           EH                                       +  KT+++GF++SSILNRI
Sbjct: 542 EH---------------------------------------QASKTKLKGFRASSILNRI 601

Query: 481 PLENFLTSTKIEALREEIILMFERDGSAKGIVLSQFTSFLDLINYSLTKSGISCVQLTGS 540
            L++F TSTKIEALREEI  M ERD SAK IV SQFTSFLDLI+Y+L KSG+SCVQL GS
Sbjct: 602 NLDDFKTSTKIEALREEIRFMVERDWSAKAIVFSQFTSFLDLISYALGKSGVSCVQLVGS 628

Query: 541 ISLAQGGDAIERFVHDPDCRILLMNLNAGGVAPNLTVASHVFIMDPWWNPAVERQALDRV 600
           +S A    A++ F  +PDCR+LLM+L AGGVA NLT ASHVF+MDPWWNPAVERQA DR+
Sbjct: 662 MSKAAKDAALKNFKEEPDCRVLLMSLQAGGVALNLTAASHVFMMDPWWNPAVERQAQDRI 628

Query: 601 SRIGAYKPIR 610
            RIG  KP+R
Sbjct: 722 HRIGQCKPVR 628

BLAST of MS005006 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 264.2 bits (674), Expect = 2.5e-70
Identity = 207/705 (29.36%), Postives = 307/705 (43.55%), Query Frame = 0

Query: 1    EVVETPSTLTMPLLGYQKAWLAWALEKE---------DSLH------------------- 60
            E +E PSTLT  L  YQK  L W  E E         ++LH                   
Sbjct: 604  EEMEAPSTLTCNLRPYQKQALYWMSESEKGIDVEKAAETLHPCWEAYRICDERAPSIYLN 663

Query: 61   -----------------RGGILADEKGMGKTIQALALVLAKRELSSIAGQRRPPN----- 120
                             RGGILAD  G+GKT+  +AL+LA+       G+  P N     
Sbjct: 664  IFSGEATIQFPTATQMARGGILADAMGLGKTVMTIALILAR------PGRGNPENEDVLV 723

Query: 121  ASSREDLPKMK-------------GTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPK 180
            A    D    K             GTL+ICP+  +S W  +++  ++  +  +LVY+G  
Sbjct: 724  ADVNADKRNRKEIHMALTTVKAKGGTLIICPMALLSQWKDELETHSKPDTVSVLVYYGGD 783

Query: 181  RAQSIESFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVK 240
            R    ++ + +D V+TTY ++   Y                      ++     I H + 
Sbjct: 784  RTHDAKAIASHDVVLTTYGVLTSAY----------------------KQDMANSIFHRID 843

Query: 241  WDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYS 300
            W RI+LDEA       +   KA F +SS  RW L   P L+ +   LYSL+CFL V P+ 
Sbjct: 844  WYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTP-LQNKLEDLYSLLCFLHVEPWC 903

Query: 301  FYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKK 360
                                        ++ WW+K I  P +N  G  R  K     L K
Sbjct: 904  ----------------------------NWAWWSKLIQKPYEN--GDPRGLK-----LIK 963

Query: 361  DILSSIVLQRTK--KDKAADLV--LPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFV 420
             IL  ++L+RTK  +DK   L+  LPP+ V +        E  FY +L   S  +F  FV
Sbjct: 964  AILRPLMLRRTKETRDKEGSLILELPPTDVQVIECEQSEAERDFYTALFKRSKVQFDQFV 1023

Query: 421  AAERETNDFAHIDDILVRLRQAVNHPYLVV-------YSKTSAI-------NCGSSTDNS 480
            A  +  +++A+I ++L+RLRQ  NHP+LV+       Y+   ++       N  S + N+
Sbjct: 1024 AQGKVLHNYANILELLLRLRQCCNHPFLVMSRADSQQYADLDSLARRFLDNNPDSVSQNA 1083

Query: 481  ----------------NEQLCGICHIAAENLVVTSCEHTFCKACMIDSTDNFWERISCPV 540
                            N + C IC  +A++ V+T C H  C+ C++ S    W   SC +
Sbjct: 1084 PSRAYIEEVIQDLRDGNSKECPICLESADDPVLTPCAHRMCRECLLTS----WRSPSCGL 1143

Query: 541  CSKKLAVDFCTTLTAGDETIKTRVEGFKSSSILNRIPLENFLTSTKIEALREEIILMFER 600
            C        C T+    E I        + SI     ++N+  S+K+  L + +  + + 
Sbjct: 1144 C------PICRTILKRTELISC-----PTDSIFRVDVVKNWKESSKVSELLKCLEKIKKS 1203

Query: 601  DGSAKGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQGGDAIERFVHDPDCRILLM 609
                K IV SQ+TSFLDL+   L + G   ++  G ++       ++ F       ILLM
Sbjct: 1204 GSGEKSIVFSQWTSFLDLLEIPLRRRGFEFLRFDGKLAQKGREKVLKEFNETKQKTILLM 1229

BLAST of MS005006 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 249.2 bits (635), Expect = 8.4e-66
Identity = 191/643 (29.70%), Postives = 299/643 (46.50%), Query Frame = 0

Query: 33  RGGILADEKGMGKTIQALALVLAKRELSSIAGQRRPPN------ASSREDL--PKMK--- 92
           RGGILAD  G+GKT+  ++L+LA    ++  G   P        +SS +DL  P +K   
Sbjct: 413 RGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISSSVDDLTSPPVKATK 472

Query: 93  ------------------GTLVICPVIAVSVWVSQIDRFTRRGSYKLLVYHGPKRAQSIE 152
                             G L++CP+  +  W ++I+   + GS  + V++G  R +  +
Sbjct: 473 FLGFDKRLLEQKSVLQNGGNLIVCPMTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAK 532

Query: 153 SFSEYDFVITTYFIVEDDYMKYLMLPKERPIQPQISKRAEGQKPCGELILHSVKWDRIIL 212
             S+ D VITTY ++  ++ +            + S   EG        +++V+W RI+L
Sbjct: 533 LLSQSDVVITTYGVLTSEFSQ------------ENSADHEG--------IYAVRWFRIVL 592

Query: 213 DEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVHPYSFYLCKD 272
           DEA       S I+ A  A+ +  RW L   P ++     LYSL+ FL++ P+       
Sbjct: 593 DEAHTIKNSKSQISLAAAALVADRRWCLTGTP-IQNNLEDLYSLLRFLRIEPWG------ 652

Query: 273 CDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRLLKKDILSSI 332
                                  + WWNK +  P +   G  R  K V  +LK      I
Sbjct: 653 ----------------------TWAWWNKLVQKPFEE--GDERGLKLVQSILK-----PI 712

Query: 333 VLQRTKKDKAAD----LVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRNFVAAERET 392
           +L+RTK     +    LVLPP+   +    L   E  FY++L   S  KF  FV   +  
Sbjct: 713 MLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYDALFKRSKVKFDQFVEQGKVL 772

Query: 393 NDFAHIDDILVRLRQAVNHPYLVV-------YSKTSAINCGSSTDNSN------------ 452
           +++A I ++L+RLRQ  +HP+LV+       YS  + ++    +  S+            
Sbjct: 773 HNYASILELLLRLRQCCDHPFLVMSRGDTAEYSDLNKLSKRFLSGKSSGLEREGKDVPSE 832

Query: 453 -------EQL-------CGICHIAAENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSK 512
                  E+L       C IC  A E+ V+T C H  C+ C++ S  N    + CPVC  
Sbjct: 833 AFVQEVVEELRKGEQGECPICLEALEDAVLTPCAHRLCRECLLASWRNSTSGL-CPVCRN 892

Query: 513 KLAVDFCTTLTAGDETIKTRVEGFKSSSILNRIPLENFLTSTKIEALREEIILMFERDGS 572
            ++     T    +   +  VE             +N++ S+KI AL EE+  +  R   
Sbjct: 893 TVSKQELITAPT-ESRFQVDVE-------------KNWVESSKITALLEELEGL--RSSG 952

Query: 573 AKGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQGGDAIERFVHDPDCRILLMNLN 610
           +K I+ SQ+T+FLDL+   L+++  S V+L G++S  Q    ++ F  D    +LLM+L 
Sbjct: 953 SKSILFSQWTAFLDLLQIPLSRNNFSFVRLDGTLSQQQREKVLKEFSEDGSILVLLMSLK 982

BLAST of MS005006 vs. TAIR 10
Match: AT1G61140.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 233.8 bits (595), Expect = 3.7e-61
Identity = 196/718 (27.30%), Postives = 313/718 (43.59%), Query Frame = 0

Query: 1    EVVETPSTLTMPLLGYQKAWLAWALEKEDSLH--RGGILADEKGMGKTIQALALVLAKR- 60
            E +     LT+PLL +Q+  L+W  +KE S     GGILAD++G+GKT+  +AL+L +R 
Sbjct: 544  EAILPDGVLTVPLLRHQRIALSWMAQKETSGFPCSGGILADDQGLGKTVSTIALILKERS 603

Query: 61   ---------------ELSSIAGQRRPPNASSR------------------EDLPKM---- 120
                           +L S  G+  P   S R                  + + K+    
Sbjct: 604  KPAQACEESTKKEIFDLESETGECAPLKPSGRSKHFEHSQLLSNENKVGGDSVGKVTGRP 663

Query: 121  -KGTLVICPVIAVSVWVSQI-DRFTRRGSYKLLVYHGPKRAQSIESFSEYDFVITTYFIV 180
              GTLV+CP   +  W  ++  + T   +  +LVYHG  R +     ++YD V+TT+ IV
Sbjct: 664  AAGTLVVCPTSVMRQWADELHKKVTSEANLSVLVYHGSSRTKDPHELAKYDVVVTTFSIV 723

Query: 181  EDDYMKYLMLP----------------------KERPIQPQISKRAEGQKPCGELI--LH 240
              +  K  ++                       K+R   P   K+   +K    L   L 
Sbjct: 724  SMEVPKQPLVDDEDEEKDGVHDGGTAATGFCSNKKRKYPPDSKKKGSKKKKVEFLSGPLA 783

Query: 241  SVKWDRIILDEARFKLFVFSNITKAIFAISSSYRWALLDGPLLEKRTRMLYSLVCFLQVH 300
             V W R++LDEA+      + + +A + + +  RW  L G  ++     LYS   FL+  
Sbjct: 784  KVSWFRVVLDEAQSIKNYKTQVARACWGLRAKRRWC-LSGTPIQNSIDDLYSYFRFLKYD 843

Query: 301  PYSFYLCKDCDCRTLDQSSLDCPKCPHKRVRHYCWWNKHITLPIQNSGGTGRDFKRVMRL 360
            PYS Y+     C T+                           PI  +   G  ++++  +
Sbjct: 844  PYSSYV---LFCSTIKN-------------------------PITRNPVKG--YQKLQAI 903

Query: 361  LKKDILSSIVLQRTK---KDKAADLVLPPSIVSIRRDTLDIQEEGFYESLHDESAAKFRN 420
            LK     +++L+RTK    D    + LPP  + +R+    ++E  FY  L  ES  +FR 
Sbjct: 904  LK-----TVMLRRTKGSLLDGKPIISLPPKSIELRKVDFTVEERDFYSKLEAESRTQFRE 963

Query: 421  FVAAERETNDFAHIDDILVRLRQAVNHPYLV--VYSKT--SAINCGSSTDNSNEQL--CG 480
            +  A     ++ +I  +L+RLRQA +HP LV   YS T  S++        S+  L  CG
Sbjct: 964  YAEAGTVKQNYVNILLMLLRLRQACDHPLLVNGEYSFTWESSVGLAKKQIQSDASLAICG 1023

Query: 481  ICHIAAENLVVTSCEHTFCKACMIDSTDNFWERISCPVCSKKLAVDFCTTLTAGDETIKT 540
            IC+ A E+ V + C H FCK C+ +             C+ +L +   ++ T  D+ +  
Sbjct: 1024 ICNDAPEDAVASVCGHVFCKQCIYERLTGDSNHCPFANCNVRLTISSLSSKTRLDDAMPD 1083

Query: 541  RVEGFKSSS---------------------ILNRIPLENFLT-STKIEALREE---IILM 600
              E   S+S                     IL  +P  + LT S +I   RE     I  
Sbjct: 1084 MQERATSNSLSPCSDEDLPYGSSKIKAALEILQSLPKAHDLTDSNQISENREYSGLSITP 1143

Query: 601  FERDGSA----------KGIVLSQFTSFLDLINYSLTKSGISCVQLTGSISLAQGGDAIE 609
             + +G +          K IV SQ+T  L+L+  SL  S I   +L G++S+A    A++
Sbjct: 1144 VKNEGMSVDVPIKVAGEKAIVFSQWTKMLNLLEASLVSSHIQYRRLDGTMSVAARDKAVQ 1203