MS004362 (gene) Bitter gourd (TR) v1

Overview
NameMS004362
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNuclear transcription factor Y subunit B
Locationscaffold92: 2242641 .. 2243857 (-)
RNA-Seq ExpressionMS004362
SyntenyMS004362
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGCTCCGACGAGTCCAGCCGGCGGCAGCCACGAGAGCGGCGGCGAGCAGAGCCCCAATACCGGTGGGGTTCGGGAGCAGGACCGATACCTCCCGATCGCTAACATTAGCCGGATCATGAAGAAGGCCTTGCCCGCTAATGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATCAGCTTCATCACTAGCGAGTGCGTCCGTCTTTGATCTCTCTTCTTTCGTATTTGGATCGTGAATTTTAGTGTCAATTCTGGTGTTAGTTTACGATTGGCGTTTCTGATACGGCAAAGGCAAATTTTTTTTTTATTGTGTATTGGATGTAATTGGTTTTGTGCGTGTGTAGGGCGAGCGATAAGTGCCAGAAGGAGAAGAGAAAGACCATTAATGGGGATGATTTGCTGTGGGCAATGGCGACATTGGGTTTCGAGGACTATATTGATCCGCTTAAGTCGTATCTAACTAGGTACAGAGAGGTAAGCTCGTTTCCCATTTTTAGCTCCTACGTGGCTTTACATTTAGAAGACTATGCACGCACGTTACTGATGCTGATAGGAGCTCTCCTCTCACGTTCTTTTACTTATTGTGATTGAGATTTGAGAGCACTTCAGTTGGTGATCAAGTGGTAAAGACTTTCCTCGGTTGATCAGTAGATGCCTAGTTCTGTTTCTTTTCAATAAGGCTATATGATTTATTTGATCTCATCACTATGATGTGCTCACATTCGTTTTCTTTTTTCCATTTTTTTTCACTTTTTTTGGTGGGGGCAATGGTGTTGCTTCAGTTGGAGGTAATTGAAACCAGCTCTTAAACCTAGTATTCTACTGGTTTATTGTACCCCCCCACCCCCCCTATACCCTAAGCTTCATTTGGGAGAAAATTATAGACTGAGACTTTTCGTTAATTATAAACTTTTACAGTGTGATGCTAAGGGATCTTCTAGGGGTGGTGATGAGTCTGCTAAAAGAGATGCAGTTGGGGCCTTGCCTGGCCAAAATTCCCAGGTTTAGTTACTTTCCATAGAATCTTGGTTTACAGAGAAATTCTATTTATTGTCATGGCCACAAGAATTGGAATTTTCTTTATATATTATGTAATCGTATCTTAAATTTTAGCATGAATATGTGCAGCAGTACATGCAGCCGGGAGCAATGACCTACATTAACACCCAAGTAATAATCTCTCTTCTC

mRNA sequence

ATGGCGGAGGCTCCGACGAGTCCAGCCGGCGGCAGCCACGAGAGCGGCGGCGAGCAGAGCCCCAATACCGGTGGGGTTCGGGAGCAGGACCGATACCTCCCGATCGCTAACATTAGCCGGATCATGAAGAAGGCCTTGCCCGCTAATGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATCAGCTTCATCACTAGCGAGGCGAGCGATAAGTGCCAGAAGGAGAAGAGAAAGACCATTAATGGGGATGATTTGCTGTGGGCAATGGCGACATTGGGTTTCGAGGACTATATTGATCCGCTTAAGTCGTATCTAACTAGGTACAGAGAGTGTGATGCTAAGGGATCTTCTAGGGGTGGTGATGAGTCTGCTAAAAGAGATGCAGTTGGGGCCTTGCCTGGCCAAAATTCCCAGCAGTACATGCAGCCGGGAGCAATGACCTACATTAACACCCAAGTAATAATCTCTCTTCTC

Coding sequence (CDS)

ATGGCGGAGGCTCCGACGAGTCCAGCCGGCGGCAGCCACGAGAGCGGCGGCGAGCAGAGCCCCAATACCGGTGGGGTTCGGGAGCAGGACCGATACCTCCCGATCGCTAACATTAGCCGGATCATGAAGAAGGCCTTGCCCGCTAATGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATCAGCTTCATCACTAGCGAGGCGAGCGATAAGTGCCAGAAGGAGAAGAGAAAGACCATTAATGGGGATGATTTGCTGTGGGCAATGGCGACATTGGGTTTCGAGGACTATATTGATCCGCTTAAGTCGTATCTAACTAGGTACAGAGAGTGTGATGCTAAGGGATCTTCTAGGGGTGGTGATGAGTCTGCTAAAAGAGATGCAGTTGGGGCCTTGCCTGGCCAAAATTCCCAGCAGTACATGCAGCCGGGAGCAATGACCTACATTAACACCCAAGTAATAATCTCTCTTCTC

Protein sequence

MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQVIISLL
Homology
BLAST of MS004362 vs. NCBI nr
Match: XP_022138911.1 (nuclear transcription factor Y subunit B-1-like isoform X3 [Momordica charantia])

HSP 1 Score: 319.3 bits (817), Expect = 1.9e-83
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 160
           KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ
Sbjct: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 159

BLAST of MS004362 vs. NCBI nr
Match: XP_022138908.1 (nuclear transcription factor Y subunit B-1-like isoform X1 [Momordica charantia] >XP_022138909.1 nuclear transcription factor Y subunit B-1-like isoform X1 [Momordica charantia])

HSP 1 Score: 314.3 bits (804), Expect = 6.2e-82
Identity = 159/161 (98.76%), Postives = 159/161 (98.76%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR--EC 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR  EC
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRELEC 120

Query: 121 DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 160
           DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ
Sbjct: 121 DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 161

BLAST of MS004362 vs. NCBI nr
Match: KAG6600098.1 (Nuclear transcription factor Y subunit B-8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.0 bits (798), Expect = 3.1e-81
Identity = 154/165 (93.33%), Postives = 162/165 (98.18%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ PTSPAGGSHESGGEQSP+TGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 40  MADPPTSPAGGSHESGGEQSPHTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 99

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 100 QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 159

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQVIISLL 166
           KGSSRGG+ESAKRDAVGALPGQNSQQY+QPGA+TYINTQ I+ ++
Sbjct: 160 KGSSRGGEESAKRDAVGALPGQNSQQYLQPGALTYINTQGILKVM 204

BLAST of MS004362 vs. NCBI nr
Match: XP_022942119.1 (nuclear transcription factor Y subunit B-10-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 310.8 bits (795), Expect = 6.9e-81
Identity = 153/160 (95.62%), Postives = 159/160 (99.38%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ PTSPAGGSHESGGEQSP+TGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 65  MADPPTSPAGGSHESGGEQSPHTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 124

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 125 QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 184

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQV 161
           KGSSRGG+ESAKRDAVGALPGQNSQQY+QPGA+TYINTQ+
Sbjct: 185 KGSSRGGEESAKRDAVGALPGQNSQQYLQPGALTYINTQL 224

BLAST of MS004362 vs. NCBI nr
Match: XP_022986143.1 (nuclear transcription factor Y subunit B-1-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 310.8 bits (795), Expect = 6.9e-81
Identity = 153/160 (95.62%), Postives = 159/160 (99.38%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ PTSPAGGSHESGGEQSP+TGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MADPPTSPAGGSHESGGEQSPHTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQV 161
           KGSSRGG+ESAKRDAVGALPGQNSQQY+QPGA+TYINTQ+
Sbjct: 121 KGSSRGGEESAKRDAVGALPGQNSQQYLQPGALTYINTQL 160

BLAST of MS004362 vs. ExPASy Swiss-Prot
Match: Q67XJ2 (Nuclear transcription factor Y subunit B-10 OS=Arabidopsis thaliana OX=3702 GN=NFYB10 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 4.4e-54
Identity = 111/148 (75.00%), Postives = 123/148 (83.11%), Query Frame = 0

Query: 1   MAEAPT-SPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDT 60
           MAE+ T    GGSHESGG+QSP +  VREQDR+LPIANISRIMK+ LP NGKIAKDAK+T
Sbjct: 1   MAESQTGGGGGGSHESGGDQSPRSLNVREQDRFLPIANISRIMKRGLPLNGKIAKDAKET 60

Query: 61  VQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC- 120
           +QECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDYIDPLK YL RYRE  
Sbjct: 61  MQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYIDPLKVYLMRYREME 120

Query: 121 -DAKGSSRGGDESAKRDAVGALPGQNSQ 146
            D KGS +GG+ SAKRD   +   Q SQ
Sbjct: 121 GDTKGSGKGGESSAKRDGQPSQVSQFSQ 148

BLAST of MS004362 vs. ExPASy Swiss-Prot
Match: Q8VYK4 (Nuclear transcription factor Y subunit B-8 OS=Arabidopsis thaliana OX=3702 GN=NFYB8 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.7e-53
Identity = 111/149 (74.50%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 1   MAEAPT-SPAG-GSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKD 60
           MAE+   SP G GSHESGG+QSP +  VREQDR+LPIANISRIMK+ LPANGKIAKDAK+
Sbjct: 1   MAESQAKSPGGCGSHESGGDQSPRSLHVREQDRFLPIANISRIMKRGLPANGKIAKDAKE 60

Query: 61  TVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC 120
            VQECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDY++PLK YL RYRE 
Sbjct: 61  IVQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREM 120

Query: 121 --DAKGSSRGGDESAKRDAVGALPGQNSQ 146
             D KGS++GGD +AK+D   +  GQ SQ
Sbjct: 121 EGDTKGSAKGGDPNAKKDGQSSQNGQFSQ 149

BLAST of MS004362 vs. ExPASy Swiss-Prot
Match: P25209 (Nuclear transcription factor Y subunit B OS=Zea mays OX=4577 GN=NFY2 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 9.1e-52
Identity = 109/149 (73.15%), Postives = 124/149 (83.22%), Query Frame = 0

Query: 1   MAEAPTSP--AGGSHESGGEQSPNTGG-VREQDRYLPIANISRIMKKALPANGKIAKDAK 60
           MAEAP SP   GGSHESG  +    GG VREQDR+LPIANISRIMKKA+PANGKIAKDAK
Sbjct: 1   MAEAPASPGGGGGSHESGSPRGGGGGGSVREQDRFLPIANISRIMKKAIPANGKIAKDAK 60

Query: 61  DTVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRE 120
           +TVQECVSEFISFITSEASDKCQ+EKRKTINGDDLLWAMATLGFEDYI+PLK YL +YRE
Sbjct: 61  ETVQECVSEFISFITSEASDKCQREKRKTINGDDLLWAMATLGFEDYIEPLKVYLQKYRE 120

Query: 121 C--DAKGSSRGGDESAKRDAVGALPGQNS 145
              D+K +++  D S K+DA+G +   +S
Sbjct: 121 MEGDSKLTAKSSDGSIKKDALGHVGASSS 149

BLAST of MS004362 vs. ExPASy Swiss-Prot
Match: Q9SLG0 (Nuclear transcription factor Y subunit B-1 OS=Arabidopsis thaliana OX=3702 GN=NFYB1 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 4.5e-51
Identity = 108/144 (75.00%), Postives = 116/144 (80.56%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ P+SPAG   ESG       G VREQDRYLPIANISRIMKKALP NGKI KDAKDTV
Sbjct: 1   MADTPSSPAGDGGESG-------GSVREQDRYLPIANISRIMKKALPPNGKIGKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC-- 120
           QECVSEFISFITSEASDKCQKEKRKT+NGDDLLWAMATLGFEDY++PLK YL RYRE   
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTVNGDDLLWAMATLGFEDYLEPLKIYLARYRELEG 120

Query: 121 DAKGSSRGGDESAKRDAVGALPGQ 143
           D KGS + GD S  RDA G + G+
Sbjct: 121 DNKGSGKSGDGS-NRDAGGGVSGE 136

BLAST of MS004362 vs. ExPASy Swiss-Prot
Match: Q60EQ4 (Nuclear transcription factor Y subunit B-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB3 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 4.5e-51
Identity = 109/156 (69.87%), Postives = 124/156 (79.49%), Query Frame = 0

Query: 1   MAEAPTSP--AGGSHES--------GGEQSPNTGGVREQDRYLPIANISRIMKKALPANG 60
           MA+ P SP   GGSHES        GG      GGVREQDR+LPIANISRIMKKA+PANG
Sbjct: 1   MADGPGSPGGGGGSHESGSPRGGGGGGGGGGGGGGVREQDRFLPIANISRIMKKAIPANG 60

Query: 61  KIAKDAKDTVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKS 120
           KIAKDAK+TVQECVSEFISFITSEASDKCQ+EKRKTINGDDLLWAMATLGFEDYI+PLK 
Sbjct: 61  KIAKDAKETVQECVSEFISFITSEASDKCQREKRKTINGDDLLWAMATLGFEDYIEPLKV 120

Query: 121 YLTRYREC--DAKGSSRGGDESAKRDAVGALPGQNS 145
           YL +YRE   D+K +++ GD S K+D +G+  G +S
Sbjct: 121 YLQKYREMEGDSKLTAKAGDGSVKKDVLGSHGGSSS 156

BLAST of MS004362 vs. ExPASy TrEMBL
Match: A0A6J1CB30 (nuclear transcription factor Y subunit B-1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009975 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 9.3e-84
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 160
           KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ
Sbjct: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 159

BLAST of MS004362 vs. ExPASy TrEMBL
Match: A0A6J1CEF2 (nuclear transcription factor Y subunit B-1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009975 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.0e-82
Identity = 159/161 (98.76%), Postives = 159/161 (98.76%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR--EC 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR  EC
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRELEC 120

Query: 121 DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 160
           DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ
Sbjct: 121 DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 161

BLAST of MS004362 vs. ExPASy TrEMBL
Match: A0A6J1JFM8 (nuclear transcription factor Y subunit B-1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111483965 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.3e-81
Identity = 153/160 (95.62%), Postives = 159/160 (99.38%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ PTSPAGGSHESGGEQSP+TGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MADPPTSPAGGSHESGGEQSPHTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQV 161
           KGSSRGG+ESAKRDAVGALPGQNSQQY+QPGA+TYINTQ+
Sbjct: 121 KGSSRGGEESAKRDAVGALPGQNSQQYLQPGALTYINTQL 160

BLAST of MS004362 vs. ExPASy TrEMBL
Match: A0A6J1FQE1 (nuclear transcription factor Y subunit B-10-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447288 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.3e-81
Identity = 153/160 (95.62%), Postives = 159/160 (99.38%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ PTSPAGGSHESGGEQSP+TGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 65  MADPPTSPAGGSHESGGEQSPHTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 124

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA
Sbjct: 125 QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 184

Query: 121 KGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQV 161
           KGSSRGG+ESAKRDAVGALPGQNSQQY+QPGA+TYINTQ+
Sbjct: 185 KGSSRGGEESAKRDAVGALPGQNSQQYLQPGALTYINTQL 224

BLAST of MS004362 vs. ExPASy TrEMBL
Match: A0A6J1CAU0 (nuclear transcription factor Y subunit B-1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009975 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.8e-80
Identity = 158/161 (98.14%), Postives = 158/161 (98.14%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV
Sbjct: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR--EC 120
           QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYR  EC
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRELEC 120

Query: 121 DAKGSSRGGDESAKRDAVGALPGQNSQQYMQPGAMTYINTQ 160
           DAKGSSRGGDESAKRDAVGALPGQNS QYMQPGAMTYINTQ
Sbjct: 121 DAKGSSRGGDESAKRDAVGALPGQNS-QYMQPGAMTYINTQ 160

BLAST of MS004362 vs. TAIR 10
Match: AT3G53340.1 (nuclear factor Y, subunit B10 )

HSP 1 Score: 212.2 bits (539), Expect = 3.1e-55
Identity = 111/148 (75.00%), Postives = 123/148 (83.11%), Query Frame = 0

Query: 1   MAEAPT-SPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDT 60
           MAE+ T    GGSHESGG+QSP +  VREQDR+LPIANISRIMK+ LP NGKIAKDAK+T
Sbjct: 1   MAESQTGGGGGGSHESGGDQSPRSLNVREQDRFLPIANISRIMKRGLPLNGKIAKDAKET 60

Query: 61  VQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC- 120
           +QECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDYIDPLK YL RYRE  
Sbjct: 61  MQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYIDPLKVYLMRYREME 120

Query: 121 -DAKGSSRGGDESAKRDAVGALPGQNSQ 146
            D KGS +GG+ SAKRD   +   Q SQ
Sbjct: 121 GDTKGSGKGGESSAKRDGQPSQVSQFSQ 148

BLAST of MS004362 vs. TAIR 10
Match: AT2G37060.1 (nuclear factor Y, subunit B8 )

HSP 1 Score: 210.3 bits (534), Expect = 1.2e-54
Identity = 111/149 (74.50%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 1   MAEAPT-SPAG-GSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKD 60
           MAE+   SP G GSHESGG+QSP +  VREQDR+LPIANISRIMK+ LPANGKIAKDAK+
Sbjct: 1   MAESQAKSPGGCGSHESGGDQSPRSLHVREQDRFLPIANISRIMKRGLPANGKIAKDAKE 60

Query: 61  TVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC 120
            VQECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDY++PLK YL RYRE 
Sbjct: 61  IVQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREM 120

Query: 121 --DAKGSSRGGDESAKRDAVGALPGQNSQ 146
             D KGS++GGD +AK+D   +  GQ SQ
Sbjct: 121 EGDTKGSAKGGDPNAKKDGQSSQNGQFSQ 149

BLAST of MS004362 vs. TAIR 10
Match: AT2G37060.2 (nuclear factor Y, subunit B8 )

HSP 1 Score: 210.3 bits (534), Expect = 1.2e-54
Identity = 111/149 (74.50%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 1   MAEAPT-SPAG-GSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKD 60
           MAE+   SP G GSHESGG+QSP +  VREQDR+LPIANISRIMK+ LPANGKIAKDAK+
Sbjct: 1   MAESQAKSPGGCGSHESGGDQSPRSLHVREQDRFLPIANISRIMKRGLPANGKIAKDAKE 60

Query: 61  TVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC 120
            VQECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDY++PLK YL RYRE 
Sbjct: 61  IVQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREM 120

Query: 121 --DAKGSSRGGDESAKRDAVGALPGQNSQ 146
             D KGS++GGD +AK+D   +  GQ SQ
Sbjct: 121 EGDTKGSAKGGDPNAKKDGQSSQNGQFSQ 149

BLAST of MS004362 vs. TAIR 10
Match: AT2G37060.3 (nuclear factor Y, subunit B8 )

HSP 1 Score: 210.3 bits (534), Expect = 1.2e-54
Identity = 111/149 (74.50%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 1   MAEAPT-SPAG-GSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKD 60
           MAE+   SP G GSHESGG+QSP +  VREQDR+LPIANISRIMK+ LPANGKIAKDAK+
Sbjct: 1   MAESQAKSPGGCGSHESGGDQSPRSLHVREQDRFLPIANISRIMKRGLPANGKIAKDAKE 60

Query: 61  TVQECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYREC 120
            VQECVSEFISF+TSEASDKCQ+EKRKTINGDDLLWAMATLGFEDY++PLK YL RYRE 
Sbjct: 61  IVQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREM 120

Query: 121 --DAKGSSRGGDESAKRDAVGALPGQNSQ 146
             D KGS++GGD +AK+D   +  GQ SQ
Sbjct: 121 EGDTKGSAKGGDPNAKKDGQSSQNGQFSQ 149

BLAST of MS004362 vs. TAIR 10
Match: AT2G38880.7 (nuclear factor Y, subunit B1 )

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-53
Identity = 108/142 (76.06%), Postives = 116/142 (81.69%), Query Frame = 0

Query: 1   MAEAPTSPAGGSHESGGEQSPNTGGVREQDRYLPIANISRIMKKALPANGKIAKDAKDTV 60
           MA+ P+SPAG   ESG       G VREQDRYLPIANISRIMKKALP NGKI KDAKDTV
Sbjct: 1   MADTPSSPAGDGGESG-------GSVREQDRYLPIANISRIMKKALPPNGKIGKDAKDTV 60

Query: 61  QECVSEFISFITSEASDKCQKEKRKTINGDDLLWAMATLGFEDYIDPLKSYLTRYRECDA 120
           QECVSEFISFITSEASDKCQKEKRKT+NGDDLLWAMATLGFEDY++PLK YL RYRE D 
Sbjct: 61  QECVSEFISFITSEASDKCQKEKRKTVNGDDLLWAMATLGFEDYLEPLKIYLARYREGDN 120

Query: 121 KGSSRGGDESAKRDAVGALPGQ 143
           KGS + GD S  RDA G + G+
Sbjct: 121 KGSGKSGDGS-NRDAGGGVSGE 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138911.11.9e-83100.00nuclear transcription factor Y subunit B-1-like isoform X3 [Momordica charantia][more]
XP_022138908.16.2e-8298.76nuclear transcription factor Y subunit B-1-like isoform X1 [Momordica charantia]... [more]
KAG6600098.13.1e-8193.33Nuclear transcription factor Y subunit B-8, partial [Cucurbita argyrosperma subs... [more]
XP_022942119.16.9e-8195.63nuclear transcription factor Y subunit B-10-like isoform X3 [Cucurbita moschata][more]
XP_022986143.16.9e-8195.63nuclear transcription factor Y subunit B-1-like isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q67XJ24.4e-5475.00Nuclear transcription factor Y subunit B-10 OS=Arabidopsis thaliana OX=3702 GN=N... [more]
Q8VYK41.7e-5374.50Nuclear transcription factor Y subunit B-8 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
P252099.1e-5273.15Nuclear transcription factor Y subunit B OS=Zea mays OX=4577 GN=NFY2 PE=2 SV=1[more]
Q9SLG04.5e-5175.00Nuclear transcription factor Y subunit B-1 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
Q60EQ44.5e-5169.87Nuclear transcription factor Y subunit B-3 OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
A0A6J1CB309.3e-84100.00nuclear transcription factor Y subunit B-1-like isoform X3 OS=Momordica charanti... [more]
A0A6J1CEF23.0e-8298.76nuclear transcription factor Y subunit B-1-like isoform X1 OS=Momordica charanti... [more]
A0A6J1JFM83.3e-8195.63nuclear transcription factor Y subunit B-1-like isoform X3 OS=Cucurbita maxima O... [more]
A0A6J1FQE13.3e-8195.63nuclear transcription factor Y subunit B-10-like isoform X3 OS=Cucurbita moschat... [more]
A0A6J1CAU02.8e-8098.14nuclear transcription factor Y subunit B-1-like isoform X2 OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT3G53340.13.1e-5575.00nuclear factor Y, subunit B10 [more]
AT2G37060.11.2e-5474.50nuclear factor Y, subunit B8 [more]
AT2G37060.21.2e-5474.50nuclear factor Y, subunit B8 [more]
AT2G37060.31.2e-5474.50nuclear factor Y, subunit B8 [more]
AT2G38880.71.7e-5376.06nuclear factor Y, subunit B1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00615CCAATSUBUNTAcoord: 60..78
score: 54.07
coord: 79..97
score: 39.35
coord: 98..116
score: 36.36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..26
NoneNo IPR availablePANTHERPTHR11064:SF134NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-2-RELATEDcoord: 1..154
IPR003958Transcription factor CBF/NF-Y/archaeal histone domainPFAMPF00808CBFD_NFYB_HMFcoord: 32..96
e-value: 5.0E-28
score: 97.1
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 21..153
e-value: 9.2E-47
score: 160.4
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 29..130
IPR027113Transcription factor NFYB/HAP3PANTHERPTHR11064CCAAT-BINDING TRANSCRIPTION FACTOR-RELATEDcoord: 1..154
IPR003956Transcription factor, NFYB/HAP3, conserved sitePROSITEPS00685NFYB_HAP3coord: 63..79

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004362.1MS004362.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016602 CCAAT-binding factor complex
cellular_component GO:0005634 nucleus
molecular_function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0043565 sequence-specific DNA binding