MS003850 (gene) Bitter gourd (TR) v1

Overview
NameMS003850
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase ATL42-like
Locationscaffold127: 944999 .. 946237 (-)
RNA-Seq ExpressionMS003850
SyntenyMS003850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAGTTTAGCAGAATCATTCTGATAATTACGGCTACTTTGCTTGTTCATGCCAATGCACAAGTGTCTCCACCAAAAGCTGATTCTGGTATTCCAGAAAACCTCACCATGCTTATTGGAATCATCTCAATCATGCTCTCCCTCGCCTTCATACTTGTTCTTTATGCGAAATTCTGTCACCGGAATTCCCGTGGACCGCGGGCTCATCTCGGAGTTGGACTTCTCAGATCAGACTCGAGATTTTCCGGGGTTGATCAGAAGGTGATTGATTCGCTTCCTTTCTTTAGATTCTCGTCGCTAAAAGGATCAAGGGAAGGGCTGGAATGTGCAGTTTGCTTATCCAAATTTGAAGATATCGAAGTTCTTCGGTTGCTGCCGAAATGCAAGCATGCCTTCCACATTGGCTGCGTTGATCATTGGCTTGAAAATCACTCGAGCTGTCCGATGTGCCGGTGTAGGATCAGCGTTGAGGACGTAGCATTCTTTACCTGTTCCAACAGTATGAGACTCATGAGGAACAACAGCCAGTCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAAGAAGATCATCACAACTCTTCAAGGTTCAACATTGGAAGCAGCTTCGGAAAAATCGGAAAGGGCAGTAAGGAAAAAGACGGAGCCAGGGAACGCCAGAGTGAGGAGCATGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACTTGAGTTCTTCTGACCTCATGTTCTTGAATACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGACACTGACAACATTCGTCGGTCGGCAACGGGAGAAACTGAGGACAAAAGGATCTCAAATATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGACTAGGATAAGCATAGCTGAGAAAAACACCTTAATTTCAATTACAGGTATGCCCTCCATATCAGATTCTAATGCAAGTACGTCAAGGACAATAAATCTTAATGAAAGAAGATCTGTCTCAGACACCACTGCTTTCTCAAGATTTGGCAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTGATTGAATCAAATGCAGAAGATTACAGAATTAGGAAGATTTGGTTGCCAATAGCAAGAAGAACAGTCCAATGGTTTGCAAATAGAGAACATAGGGCTCAACTTTCAAAGTACACACAGCAGCCAGATGTA

mRNA sequence

ATGAGTAAGTTTAGCAGAATCATTCTGATAATTACGGCTACTTTGCTTGTTCATGCCAATGCACAAGTGTCTCCACCAAAAGCTGATTCTGGTATTCCAGAAAACCTCACCATGCTTATTGGAATCATCTCAATCATGCTCTCCCTCGCCTTCATACTTGTTCTTTATGCGAAATTCTGTCACCGGAATTCCCGTGGACCGCGGGCTCATCTCGGAGTTGGACTTCTCAGATCAGACTCGAGATTTTCCGGGGTTGATCAGAAGGTGATTGATTCGCTTCCTTTCTTTAGATTCTCGTCGCTAAAAGGATCAAGGGAAGGGCTGGAATGTGCAGTTTGCTTATCCAAATTTGAAGATATCGAAGTTCTTCGGTTGCTGCCGAAATGCAAGCATGCCTTCCACATTGGCTGCGTTGATCATTGGCTTGAAAATCACTCGAGCTGTCCGATGTGCCGGTGTAGGATCAGCGTTGAGGACGTAGCATTCTTTACCTGTTCCAACAGTATGAGACTCATGAGGAACAACAGCCAGTCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAAGAAGATCATCACAACTCTTCAAGGTTCAACATTGGAAGCAGCTTCGGAAAAATCGGAAAGGGCAGTAAGGAAAAAGACGGAGCCAGGGAACGCCAGAGTGAGGAGCATGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACTTGAGTTCTTCTGACCTCATGTTCTTGAATACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGACACTGACAACATTCGTCGGTCGGCAACGGGAGAAACTGAGGACAAAAGGATCTCAAATATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGACTAGGATAAGCATAGCTGAGAAAAACACCTTAATTTCAATTACAGGTATGCCCTCCATATCAGATTCTAATGCAAGTACGTCAAGGACAATAAATCTTAATGAAAGAAGATCTGTCTCAGACACCACTGCTTTCTCAAGATTTGGCAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTGATTGAATCAAATGCAGAAGATTACAGAATTAGGAAGATTTGGTTGCCAATAGCAAGAAGAACAGTCCAATGGTTTGCAAATAGAGAACATAGGGCTCAACTTTCAAAGTACACACAGCAGCCAGATGTA

Coding sequence (CDS)

ATGAGTAAGTTTAGCAGAATCATTCTGATAATTACGGCTACTTTGCTTGTTCATGCCAATGCACAAGTGTCTCCACCAAAAGCTGATTCTGGTATTCCAGAAAACCTCACCATGCTTATTGGAATCATCTCAATCATGCTCTCCCTCGCCTTCATACTTGTTCTTTATGCGAAATTCTGTCACCGGAATTCCCGTGGACCGCGGGCTCATCTCGGAGTTGGACTTCTCAGATCAGACTCGAGATTTTCCGGGGTTGATCAGAAGGTGATTGATTCGCTTCCTTTCTTTAGATTCTCGTCGCTAAAAGGATCAAGGGAAGGGCTGGAATGTGCAGTTTGCTTATCCAAATTTGAAGATATCGAAGTTCTTCGGTTGCTGCCGAAATGCAAGCATGCCTTCCACATTGGCTGCGTTGATCATTGGCTTGAAAATCACTCGAGCTGTCCGATGTGCCGGTGTAGGATCAGCGTTGAGGACGTAGCATTCTTTACCTGTTCCAACAGTATGAGACTCATGAGGAACAACAGCCAGTCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAAGAAGATCATCACAACTCTTCAAGGTTCAACATTGGAAGCAGCTTCGGAAAAATCGGAAAGGGCAGTAAGGAAAAAGACGGAGCCAGGGAACGCCAGAGTGAGGAGCATGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACTTGAGTTCTTCTGACCTCATGTTCTTGAATACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGACACTGACAACATTCGTCGGTCGGCAACGGGAGAAACTGAGGACAAAAGGATCTCAAATATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGACTAGGATAAGCATAGCTGAGAAAAACACCTTAATTTCAATTACAGGTATGCCCTCCATATCAGATTCTAATGCAAGTACGTCAAGGACAATAAATCTTAATGAAAGAAGATCTGTCTCAGACACCACTGCTTTCTCAAGATTTGGCAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTGATTGAATCAAATGCAGAAGATTACAGAATTAGGAAGATTTGGTTGCCAATAGCAAGAAGAACAGTCCAATGGTTTGCAAATAGAGAACATAGGGCTCAACTTTCAAAGTACACACAGCAGCCAGATGTA

Protein sequence

MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNSRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV
Homology
BLAST of MS003850 vs. NCBI nr
Match: XP_022144842.1 (E3 ubiquitin-protein ligase ATL42-like [Momordica charantia])

HSP 1 Score: 801.6 bits (2069), Expect = 3.2e-228
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC 60
           MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC
Sbjct: 1   MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC 60

Query: 61  HRNSRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI 120
           HRNSRGPRAHLGVGLLRSD RFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI
Sbjct: 61  HRNSRGPRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI 120

Query: 121 EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL 180
           EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL
Sbjct: 121 EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL 180

Query: 181 PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL 240
           PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL
Sbjct: 181 PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL 240

Query: 241 VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKE 300
           VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRS TGETEDKRISNIKE
Sbjct: 241 VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETEDKRISNIKE 300

Query: 301 EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF 360
           EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF
Sbjct: 301 EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF 360

Query: 361 NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV 414
           NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV
Sbjct: 361 NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV 413

BLAST of MS003850 vs. NCBI nr
Match: XP_031738779.1 (putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetical protein Csa_022677 [Cucumis sativus])

HSP 1 Score: 602.1 bits (1551), Expect = 3.7e-168
Identity = 321/422 (76.07%), Postives = 363/422 (86.02%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60
           M KFSRII+II    L    AQ  SPPKA SGIP+N T++IGI+SIM S+ FILV+YAKF
Sbjct: 1   MGKFSRIIMIILTIFLFPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHRNS--RG-PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  S  RG P+AHLG GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLS 
Sbjct: 61  CHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSN 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRI VED+AFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAFFK-SNSIRLIRNNS 180

Query: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD-----GARERQSEEHVV 240
           +SELPQD  +ELFVQREE+HHNSSR N G+SF  IGKG+KEK+      A E +SEE V+
Sbjct: 181 RSELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVL 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETE 300
           FHKHKHKILVSDFVFMNRWSNLSSSDL+FLNTEMLG MSSNRFSSLD+++I +SAT E E
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKE 300

Query: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSI-SDSNASTSRTINLNERRSVSD 360
            K+ S IKEEMEMKRSFE RISIAEKN+L+SI+G+ S  SDSNASTSRT  LNERRSVSD
Sbjct: 301 GKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDSNASTSRTTILNERRSVSD 360

Query: 361 TTAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQ 413
           TTAFSRFG FNENA R++S+IE++A+ +RIR++WLPIARRTVQWFANRE R +LSKYTQQ
Sbjct: 361 TTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQ 420

BLAST of MS003850 vs. NCBI nr
Match: XP_031742649.1 (E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 9.5e-164
Identity = 308/398 (77.39%), Postives = 349/398 (87.69%), Query Frame = 0

Query: 24  SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNS--RG-PRAHLGVGLLRSDS 83
           SPPKA SGIP+N T++IGI+SIM S+ FILV+YAKFCH  S  RG P+AHLG GLLRSDS
Sbjct: 13  SPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKFCHPTSHFRGEPQAHLGHGLLRSDS 72

Query: 84  RFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCVDH 143
           RFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGC+DH
Sbjct: 73  RFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDH 132

Query: 144 WLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNMELFVQREEDHHNSS 203
           WLE HSSCP+CRCRI VED+AFF  SNS+RL+RNNS+ ELPQD  +ELFVQREE+HHNSS
Sbjct: 133 WLEKHSSCPICRCRIGVEDIAFFK-SNSIRLIRNNSRPELPQDSRIELFVQREENHHNSS 192

Query: 204 RFNIGSSFGKIGKGSKEKD-----GARERQSEEHVVFHKHKHKILVSDFVFMNRWSNLSS 263
           R N G+SF  IGKG+KEK+      A E +SEE V+FHKHKHKILVSDFVFMNRWSNLSS
Sbjct: 193 RSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVLFHKHKHKILVSDFVFMNRWSNLSS 252

Query: 264 SDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKEEMEMKRSFETRISIA 323
           SDL+FLNTEMLG MSSNRFSSLD+++I +SAT E E K+ S IKEEMEMKRSFE RISIA
Sbjct: 253 SDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKEGKKTSQIKEEMEMKRSFERRISIA 312

Query: 324 EKNTLISITGMPSI-SDSNASTSRTINLNERRSVSDTTAFSRFGNFNENAIRETSVIESN 383
           EKN+L+SI+G+ S  SD NASTSRT  LNERRSVSDTTAFSRFG FNENA R++S+IE++
Sbjct: 313 EKNSLVSISGISSTSSDPNASTSRTTILNERRSVSDTTAFSRFGYFNENATRDSSMIEAD 372

Query: 384 AEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPD 413
           A+ +RIR++WLPIARRTVQWFANRE R +LSKYTQQPD
Sbjct: 373 AKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQPD 409

BLAST of MS003850 vs. NCBI nr
Match: KAE8647036.1 (hypothetical protein Csa_023000 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 6.8e-154
Identity = 292/376 (77.66%), Postives = 329/376 (87.50%), Query Frame = 0

Query: 46  MLSLAFILVLYAKFCHRNS--RG-PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLK 105
           M S+ FILV+YAKFCH  S  RG P+AHLG GLLRSDSRFSGVDQKVID+LPFFRFSSLK
Sbjct: 1   MFSMVFILVVYAKFCHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLK 60

Query: 106 GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAF 165
           GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRI VED+AF
Sbjct: 61  GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAF 120

Query: 166 FTCSNSMRLMRNNSQSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD--- 225
           F  SNS+RL+RNNS+ ELPQD  +ELFVQREE+HHNSSR N G+SF  IGKG+KEK+   
Sbjct: 121 FK-SNSIRLIRNNSRPELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLI 180

Query: 226 --GARERQSEEHVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSL 285
              A E +SEE V+FHKHKHKILVSDFVFMNRWSNLSSSDL+FLNTEMLG MSSNRFSSL
Sbjct: 181 ENEAGECESEERVLFHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSL 240

Query: 286 DTDNIRRSATGETEDKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSI-SDSNAST 345
           D+++I +SAT E E K+ S IKEEMEMKRSFE RISIAEKN+L+SI+G+ S  SD NAST
Sbjct: 241 DSESISQSATREKEGKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDPNAST 300

Query: 346 SRTINLNERRSVSDTTAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFA 405
           SRT  LNERRSVSDTTAFSRFG FNENA R++S+IE++A+ +RIR++WLPIARRTVQWFA
Sbjct: 301 SRTTILNERRSVSDTTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFA 360

Query: 406 NREHRAQLSKYTQQPD 413
           NRE R +LSKYTQQPD
Sbjct: 361 NREQRFRLSKYTQQPD 375

BLAST of MS003850 vs. NCBI nr
Match: KAA0046520.1 (E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa])

HSP 1 Score: 514.2 bits (1323), Expect = 1.0e-141
Identity = 285/416 (68.51%), Postives = 328/416 (78.85%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60
           M +F+RII+II    L    AQ  SPPKA S IP+N T++IGI+SIM S+ FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHRNS--RG-PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  S  RG P+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRISVEDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHK 240
           Q +LPQD +MELFV REE+HH SSR N G+SF KIGKGSKEK+   E ++          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 HKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRIS 300
                                      EMLGAMSS+RFSSL++++IRRSAT E E KR S
Sbjct: 241 ---------------------------EMLGAMSSSRFSSLESESIRRSATREREGKRTS 300

Query: 301 NIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSR 360
            IKE MEMKRSFE RISIAEKN+L+SI+G+ S SDSNASTS+T NLNE RSVSDTTAFSR
Sbjct: 301 QIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDTTAFSR 360

Query: 361 FGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPD 413
           FG FNENAIR++S+IE++A+ YRIR++WLPIARRTVQWFANRE R++LSKYTQQPD
Sbjct: 361 FGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 377

BLAST of MS003850 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 7.7e-84
Identity = 200/446 (44.84%), Postives = 278/446 (62.33%), Query Frame = 0

Query: 6   RIILIITATLLVHANAQVSPPKADSGIPEN----LTMLIGIISIMLSLAFILVLYAKFCH 65
           +I       L  +A+AQ  PP  +  +  N    L ++ G+++IM +L F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ---------RNSRGPRAHLGVGLLR---SDSRFSGVDQKVIDSLPFFRFSSLKGSREGLE 125
                    R     R   G+   R   S  RFSG+D+  I+SLP FRFS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISV-EDVAFFTCSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGC+D WLE H++CP+CR R+S+ ED +  T  NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRLMRNNSQSELPQDPNMELFVQREEDHH--------NSSRFNIGSSFGKIGK-GSKEKD 245
            R +   +QSE+ +D ++EL+++REE+           SSRF+IG SF KI K G+KEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 GARE--RQSEEHVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSL 305
              E     +E  + HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RFSSL
Sbjct: 243 LLDEHVNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERFSSL 302

Query: 306 DTDNIRRSATGETEDK-RISNIKEEMEMKRSFETRISIAEKNTLISITGM-PSISDSNAS 365
             D+++R   G+ ED+  I  IKEEME KR  E        N L S+T M  S +  + S
Sbjct: 303 --DHVKR---GDEEDQIGILRIKEEMEAKRMLE--------NKLTSMTTMFSSENGDSGS 362

Query: 366 TSRTINLNE-RRSVSDTTAFSRF-----GNFNENAIRETSVIES--NAEDYRIRKIWLPI 414
            SR++ +   RRSVSD TA  R      G+ + +A    S +++  N  + R R++WLPI
Sbjct: 363 KSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLPI 422

BLAST of MS003850 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.9e-69
Identity = 176/426 (41.31%), Postives = 241/426 (56.57%), Query Frame = 0

Query: 8   ILIITATLLVHANAQVSPPK---ADSGI-PENLTMLIGIISIMLSLAFILVLYAKFCHRN 67
           IL      L + +AQ  PP    A S +   +L ++ G+ SI+ +L F+L++YAK  H +
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHND 68

Query: 68  SR------GPRA---HLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCL 127
            R      G R     L  GL    SRFSG+D+K I+SLPFFRFS+LKG ++GLEC+VCL
Sbjct: 69  LRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCL 128

Query: 128 SKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRN 187
           SKFED+E+LRLLPKC+HAFHIGC+D WLE H++CP+CR R+++ED      ++S  L   
Sbjct: 129 SKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRIL 188

Query: 188 NSQSELPQDPNMELFVQREE-DHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFH 247
           N      +D  +E++++REE  +  SSRF   SSF KI K S   +       +E  + H
Sbjct: 189 NQSETREEDSRLEIYIEREEGTNDGSSRF---SSFRKILKKSLLLEREGNENIDEKKLMH 248

Query: 248 KHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDK 307
           K  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+D     R   G   D 
Sbjct: 249 KFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSVD-----RVHRGNLRD- 308

Query: 308 RISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTA 367
                KE+MEMK     R+ I  K+                        + RR+VS+ T 
Sbjct: 309 -----KEDMEMK-----RMLIKHKD------------------------SSRRTVSEITT 368

Query: 368 FSR---FGNFNENAIRETS---VIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKY 414
            SR    G     +   TS    + +  E+ R R++WLPIARRT QWF NRE    L+  
Sbjct: 369 VSREKAVGGSYRGSTASTSQNYAVTATTEERR-RRLWLPIARRTAQWFVNREKSNDLNTT 390

BLAST of MS003850 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 6.9e-32
Identity = 104/332 (31.33%), Postives = 156/332 (46.99%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQVSPP----------KADSGIPENLTMLIGIISIMLSLA 60
           +S F  + L    T +V   +   PP             S +   + ++I +++   SL 
Sbjct: 13  LSLFLNVSLADNHTAVVITTSDTPPPLPPPSPPPRHNFTSSLMPGIAVVIAVLTAFFSLT 72

Query: 61  FILVLYAKFCHRN--------------SRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFF 120
           F+L+LY K C R               +R    +   G +    + SG+D+ VI+SLP F
Sbjct: 73  FLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGGRKNSGIDRSVIESLPVF 132

Query: 121 RFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRIS 180
           RF +L G ++GLECAVCL++FE  EVLRLLPKCKHAFH+ CVD WL+ HS+CP+CR R+ 
Sbjct: 133 RFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVD 192

Query: 181 VEDVAFFTCSNS---MRLMRNNSQSELPQDPNMELF--VQREEDHHNS-----SRFN--- 240
            ED+      NS   ++  ++ S S     P +  F  V R    H+S     SR N   
Sbjct: 193 PEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPVSRISGRHSSAGERASRLNEIR 252

Query: 241 -------IGSSFGKIGKGSKEKDGARERQSEEHVV---------------------FHKH 267
                     SF +    S + + A E +SE   V                       + 
Sbjct: 253 TSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRKDGLLLIPNRESFEGRF 312

BLAST of MS003850 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 107.5 bits (267), Expect = 3.8e-22
Identity = 58/159 (36.48%), Postives = 88/159 (55.35%), Query Frame = 0

Query: 7   IILIITATLLVHANAQ---VSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHR- 66
           IIL +   +   ANAQ    SPP   +G   + T +  ++  +  L  +L +Y + C R 
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARS 73

Query: 67  NSRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREG---LECAVCLSKFED 126
           N      +         SR  G+D  V++S P F +SS+K S+ G   LECA+CL++ ED
Sbjct: 74  NPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELED 133

Query: 127 IEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVE 159
            E +RLLP C H FHI C+D WL +H++CP+CR  ++ +
Sbjct: 134 HETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of MS003850 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-21
Identity = 72/224 (32.14%), Postives = 112/224 (50.00%), Query Frame = 0

Query: 16  LVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNSRGPRAHLGVGL 75
           L+   +  S    +S I  N+ ++I I+SI+  ++ +L +  KF    SR  R      +
Sbjct: 23  LIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESREDYFDNV 82

Query: 76  LRSDSRF--------SGVDQKVIDSLPFFRFSSLKGSR-EGLECAVCLSKFEDIEVLRLL 135
                +         SGVDQ +ID+LP F + S+ G +    +C VCL +FE  + LRLL
Sbjct: 83  TALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFETEDKLRLL 142

Query: 136 PKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNM 195
           PKC HAFH+ C+D WL +HS+CP+CR  +     +    S+S  L+  + QS     P +
Sbjct: 143 PKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSSRDMVPVL 202

Query: 196 ELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHV 231
           E   Q   D +N S     S+  + G+ S + DG  +   E+ V
Sbjct: 203 ESNSQLGYDVNNDSE----STRIRSGRKSCDPDGDMDGLDEKVV 242

BLAST of MS003850 vs. ExPASy TrEMBL
Match: A0A6J1CUV0 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014428 PE=4 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.6e-228
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC 60
           MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC
Sbjct: 1   MSKFSRIILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFC 60

Query: 61  HRNSRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI 120
           HRNSRGPRAHLGVGLLRSD RFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI
Sbjct: 61  HRNSRGPRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDI 120

Query: 121 EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL 180
           EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL
Sbjct: 121 EVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSEL 180

Query: 181 PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL 240
           PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL
Sbjct: 181 PQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHKHKIL 240

Query: 241 VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKE 300
           VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRS TGETEDKRISNIKE
Sbjct: 241 VSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETEDKRISNIKE 300

Query: 301 EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF 360
           EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF
Sbjct: 301 EMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSRFGNF 360

Query: 361 NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV 414
           NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV
Sbjct: 361 NENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPDV 413

BLAST of MS003850 vs. ExPASy TrEMBL
Match: A0A5D3DZA7 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00780 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 5.0e-142
Identity = 285/416 (68.51%), Postives = 328/416 (78.85%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60
           M +F+RII+II    L    AQ  SPPKA S IP+N T++IGI+SIM S+ FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHRNS--RG-PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  S  RG P+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRISVEDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHK 240
           Q +LPQD +MELFV REE+HH SSR N G+SF KIGKGSKEK+   E ++          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 HKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRIS 300
                                      EMLGAMSS+RFSSL++++IRRSAT E E KR S
Sbjct: 241 ---------------------------EMLGAMSSSRFSSLESESIRRSATREREGKRTS 300

Query: 301 NIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSR 360
            IKE MEMKRSFE RISIAEKN+L+SI+G+ S SDSNASTS+T NLNE RSVSDTTAFSR
Sbjct: 301 QIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDTTAFSR 360

Query: 361 FGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPD 413
           FG FNENAIR++S+IE++A+ YRIR++WLPIARRTVQWFANRE R++LSKYTQQPD
Sbjct: 361 FGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 377

BLAST of MS003850 vs. ExPASy TrEMBL
Match: A0A1S3CHH4 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 7.4e-138
Identity = 279/416 (67.07%), Postives = 319/416 (76.68%), Query Frame = 0

Query: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60
           M +F+RII+II    L    AQ  SPPKA S IP+N T++IGI+SIM S+ FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHRNS--RG-PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  S  RG P+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRISVEDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFHKHK 240
           Q +LPQD +MELFV REE+HH SSR N G+SF KIGK                       
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGK----------------------- 240

Query: 241 HKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRIS 300
                                      EMLGAMSS+RFSSL++++IRRSAT E E KR S
Sbjct: 241 ---------------------------EMLGAMSSSRFSSLESESIRRSATREREGKRTS 300

Query: 301 NIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTAFSR 360
            IKE MEMKRSFE RISIAEKN+L+SI+G+ S SDSNASTS+T NLNE RSVSDTTAFSR
Sbjct: 301 QIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDTTAFSR 360

Query: 361 FGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQPD 413
           FG FNENAIR++S+IE++A+ YRIR++WLPIARRTVQWFANRE R++LSKYTQQPD
Sbjct: 361 FGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 364

BLAST of MS003850 vs. ExPASy TrEMBL
Match: A0A2N9GBZ7 (RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24653 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 5.2e-115
Identity = 230/413 (55.69%), Postives = 297/413 (71.91%), Query Frame = 0

Query: 7   IILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNSRG 66
           +++ +      H  AQ +     S  P +LT++IGI+S+M SL  +L++YAKFCHR    
Sbjct: 6   LLIFVLHFFFFHVKAQYTGD--GSASPGSLTVVIGILSLMFSLTILLLVYAKFCHRRGPI 65

Query: 67  PRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLL 126
                   L+RS SRFSG+D+ VI+SLPFFRFSSLKGSR+GLECAVCLSKFED E+LRLL
Sbjct: 66  HSDPQNFALIRSSSRFSGIDKTVIESLPFFRFSSLKGSRDGLECAVCLSKFEDTEILRLL 125

Query: 127 PKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNM 186
           PKCKHAFHI C+D+WLE H+SCP+CR ++S ED + FT +NSMRL+   +QSEL QD N+
Sbjct: 126 PKCKHAFHIECIDNWLEKHASCPLCRHKVSAEDPSIFTYTNSMRLI---NQSELRQDSNI 185

Query: 187 ELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQS-----EEHVVFHKHKHKILV 246
           ELFVQREEDHH SSRF+IG+SF K  KG KE++   + ++     E H+V HKH HKI+V
Sbjct: 186 ELFVQREEDHHGSSRFSIGNSFRKTEKGDKEENPLIQEEADNSFDENHIVLHKHNHKIIV 245

Query: 247 SDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKEE 306
            DFVF NRWSNLSSSDLMFLN+EMLGA SS RF+S D +N + S   E E   I  IKEE
Sbjct: 246 PDFVFKNRWSNLSSSDLMFLNSEMLGATSSYRFASFDLNNEQSSTARENE--HIVKIKEE 305

Query: 307 MEMKRSFETRISIAEKNTLISITGMPSISDSNAS---TSRTINLNERRSVSDTTAFSRFG 366
           M+MKR FE ++S   K + +S  G+PS SDS ++   TSR +N  ERRS+S+ TA SR+ 
Sbjct: 306 MDMKRLFENKVSTLNKTSAVSFPGIPSTSDSTSNLSQTSRYMNPTERRSMSEITALSRYE 365

Query: 367 NFN-ENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQ 411
           +   +N I E S++ +N +  R+R++WLPIARRTV W+ANRE R+Q S+ TQQ
Sbjct: 366 DSGMKNRIGEASLLGNNVKAERMRRLWLPIARRTVHWYANRERRSQQSQNTQQ 411

BLAST of MS003850 vs. ExPASy TrEMBL
Match: A0A7N2KVY2 (RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 2.0e-114
Identity = 226/413 (54.72%), Postives = 298/413 (72.15%), Query Frame = 0

Query: 8   ILIITATLLVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNSRGP 67
           +++   +++  +NA      A   +  +L ++IGI+SIM SL F+L++YAKFCHR     
Sbjct: 4   VIVSADSVVDDSNADGDNQAASQPVQPSLAVVIGILSIMFSLTFLLLVYAKFCHRRGSIH 63

Query: 68  RAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLP 127
                 G +RS SRFSG+D+ VI+SLPFFRFSSLKG++EGLEC+VCLSKFED E+LRLLP
Sbjct: 64  SDPQNFGFIRSSSRFSGIDKTVIESLPFFRFSSLKGTKEGLECSVCLSKFEDTEMLRLLP 123

Query: 128 KCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNME 187
           KCKHAFHI C+D+WL  HSSCP+CR ++S ED   FT +NSMRLM   +QSEL QD N+E
Sbjct: 124 KCKHAFHIDCIDNWLIKHSSCPLCRHKVSPEDPTIFTYTNSMRLM---NQSELRQDSNIE 183

Query: 188 LFVQREEDHHNSSRFNIGSSFGKIGKGSKEK-----DGARERQSEEHVVFHKHKHKILVS 247
           LFVQREEDHH SSRF+IGSSF K  KG+ E+         +   E + V HKH HKI+VS
Sbjct: 184 LFVQREEDHHGSSRFSIGSSFRKTEKGNTEEKPLIFQEEADNNDENNKVLHKHNHKIIVS 243

Query: 248 DFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDKRISNIKEEM 307
           DF+F NRWS++SSSDLMFLN+E+L A SS RFSSLD +N + S     E+  I  IKEEM
Sbjct: 244 DFIFNNRWSSVSSSDLMFLNSELLSATSSYRFSSLDWNNEQSSTPRSLENGHIVKIKEEM 303

Query: 308 EMKRSFETRISIAEKNTLISITGMPSISDSN---ASTSRTINLNERRSVSDTTAFSRFGN 367
           E+KRSFET++S   K + +S  G+PS SDS    + TSR +N  ERRS+S+ TA SR+ +
Sbjct: 304 ELKRSFETKVSTLNKTSPVSFPGLPSTSDSTPNLSQTSRYMNPTERRSMSEITALSRYED 363

Query: 368 FN--ENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQ 411
            +  +N IRE+S+IE+N +  R+R++W PIA+RTV+W+ANRE R+Q S+ TQ+
Sbjct: 364 ISGIKNRIRESSLIENNVKAERMRRLWFPIAKRTVKWYANRERRSQQSQNTQR 413

BLAST of MS003850 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 5.5e-85
Identity = 200/446 (44.84%), Postives = 278/446 (62.33%), Query Frame = 0

Query: 6   RIILIITATLLVHANAQVSPPKADSGIPEN----LTMLIGIISIMLSLAFILVLYAKFCH 65
           +I       L  +A+AQ  PP  +  +  N    L ++ G+++IM +L F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ---------RNSRGPRAHLGVGLLR---SDSRFSGVDQKVIDSLPFFRFSSLKGSREGLE 125
                    R     R   G+   R   S  RFSG+D+  I+SLP FRFS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISV-EDVAFFTCSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGC+D WLE H++CP+CR R+S+ ED +  T  NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRLMRNNSQSELPQDPNMELFVQREEDHH--------NSSRFNIGSSFGKIGK-GSKEKD 245
            R +   +QSE+ +D ++EL+++REE+           SSRF+IG SF KI K G+KEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 GARE--RQSEEHVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSL 305
              E     +E  + HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RFSSL
Sbjct: 243 LLDEHVNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERFSSL 302

Query: 306 DTDNIRRSATGETEDK-RISNIKEEMEMKRSFETRISIAEKNTLISITGM-PSISDSNAS 365
             D+++R   G+ ED+  I  IKEEME KR  E        N L S+T M  S +  + S
Sbjct: 303 --DHVKR---GDEEDQIGILRIKEEMEAKRMLE--------NKLTSMTTMFSSENGDSGS 362

Query: 366 TSRTINLNE-RRSVSDTTAFSRF-----GNFNENAIRETSVIES--NAEDYRIRKIWLPI 414
            SR++ +   RRSVSD TA  R      G+ + +A    S +++  N  + R R++WLPI
Sbjct: 363 KSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLPI 422

BLAST of MS003850 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.3e-70
Identity = 176/426 (41.31%), Postives = 241/426 (56.57%), Query Frame = 0

Query: 8   ILIITATLLVHANAQVSPPK---ADSGI-PENLTMLIGIISIMLSLAFILVLYAKFCHRN 67
           IL      L + +AQ  PP    A S +   +L ++ G+ SI+ +L F+L++YAK  H +
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHND 68

Query: 68  SR------GPRA---HLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCL 127
            R      G R     L  GL    SRFSG+D+K I+SLPFFRFS+LKG ++GLEC+VCL
Sbjct: 69  LRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCL 128

Query: 128 SKFEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRN 187
           SKFED+E+LRLLPKC+HAFHIGC+D WLE H++CP+CR R+++ED      ++S  L   
Sbjct: 129 SKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRIL 188

Query: 188 NSQSELPQDPNMELFVQREE-DHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHVVFH 247
           N      +D  +E++++REE  +  SSRF   SSF KI K S   +       +E  + H
Sbjct: 189 NQSETREEDSRLEIYIEREEGTNDGSSRF---SSFRKILKKSLLLEREGNENIDEKKLMH 248

Query: 248 KHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSATGETEDK 307
           K  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+D     R   G   D 
Sbjct: 249 KFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSVD-----RVHRGNLRD- 308

Query: 308 RISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDTTA 367
                KE+MEMK     R+ I  K+                        + RR+VS+ T 
Sbjct: 309 -----KEDMEMK-----RMLIKHKD------------------------SSRRTVSEITT 368

Query: 368 FSR---FGNFNENAIRETS---VIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKY 414
            SR    G     +   TS    + +  E+ R R++WLPIARRT QWF NRE    L+  
Sbjct: 369 VSREKAVGGSYRGSTASTSQNYAVTATTEERR-RRLWLPIARRTAQWFVNREKSNDLNTT 390

BLAST of MS003850 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.4e-32
Identity = 96/287 (33.45%), Postives = 143/287 (49.83%), Query Frame = 0

Query: 36  LTMLIGIISIMLSLAFILVLYAKFCHRN--------------SRGPRAHLGVGLLRSDSR 95
           + ++I +++   SL F+L+LY K C R               +R    +   G +    +
Sbjct: 4   IAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGGRK 63

Query: 96  FSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCVDHW 155
            SG+D+ VI+SLP FRF +L G ++GLECAVCL++FE  EVLRLLPKCKHAFH+ CVD W
Sbjct: 64  NSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTW 123

Query: 156 LENHSSCPMCRCRISVEDVAFFTCSNS---MRLMRNNSQSELPQDPNMELF--VQREEDH 215
           L+ HS+CP+CR R+  ED+      NS   ++  ++ S S     P +  F  V R    
Sbjct: 124 LDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPVSRISGR 183

Query: 216 HNS-----SRFN----------IGSSFGKIGKGSKEKDGARERQSEEHVV---------- 267
           H+S     SR N             SF +    S + + A E +SE   V          
Sbjct: 184 HSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRKD 243

BLAST of MS003850 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-23
Identity = 58/159 (36.48%), Postives = 88/159 (55.35%), Query Frame = 0

Query: 7   IILIITATLLVHANAQ---VSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHR- 66
           IIL +   +   ANAQ    SPP   +G   + T +  ++  +  L  +L +Y + C R 
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARS 73

Query: 67  NSRGPRAHLGVGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREG---LECAVCLSKFED 126
           N      +         SR  G+D  V++S P F +SS+K S+ G   LECA+CL++ ED
Sbjct: 74  NPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELED 133

Query: 127 IEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVE 159
            E +RLLP C H FHI C+D WL +H++CP+CR  ++ +
Sbjct: 134 HETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of MS003850 vs. TAIR 10
Match: AT2G18650.1 (RING/U-box superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 7.8e-23
Identity = 72/224 (32.14%), Postives = 112/224 (50.00%), Query Frame = 0

Query: 16  LVHANAQVSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKFCHRNSRGPRAHLGVGL 75
           L+   +  S    +S I  N+ ++I I+SI+  ++ +L +  KF    SR  R      +
Sbjct: 23  LIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESREDYFDNV 82

Query: 76  LRSDSRF--------SGVDQKVIDSLPFFRFSSLKGSR-EGLECAVCLSKFEDIEVLRLL 135
                +         SGVDQ +ID+LP F + S+ G +    +C VCL +FE  + LRLL
Sbjct: 83  TALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFETEDKLRLL 142

Query: 136 PKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNSQSELPQDPNM 195
           PKC HAFH+ C+D WL +HS+CP+CR  +     +    S+S  L+  + QS     P +
Sbjct: 143 PKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSSRDMVPVL 202

Query: 196 ELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQSEEHV 231
           E   Q   D +N S     S+  + G+ S + DG  +   E+ V
Sbjct: 203 ESNSQLGYDVNNDSE----STRIRSGRKSCDPDGDMDGLDEKVV 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144842.13.2e-22899.52E3 ubiquitin-protein ligase ATL42-like [Momordica charantia][more]
XP_031738779.13.7e-16876.07putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetic... [more]
XP_031742649.19.5e-16477.39E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus][more]
KAE8647036.16.8e-15477.66hypothetical protein Csa_023000 [Cucumis sativus][more]
KAA0046520.11.0e-14168.51E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3... [more]
Match NameE-valueIdentityDescription
Q5XF857.7e-8444.84E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ... [more]
Q9SL781.9e-6941.31Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
Q5EAE96.9e-3231.33RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q8W5713.8e-2236.48RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Q9ZV531.1e-2132.14Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 P... [more]
Match NameE-valueIdentityDescription
A0A6J1CUV01.6e-22899.52E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3DZA75.0e-14268.51E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHH47.4e-13867.07E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 P... [more]
A0A2N9GBZ75.2e-11555.69RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2465... [more]
A0A7N2KVY22.0e-11454.72RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G28890.15.5e-8544.84RING/U-box superfamily protein [more]
AT2G20030.11.3e-7041.31RING/U-box superfamily protein [more]
AT5G05810.12.4e-3233.45RING/U-box superfamily protein [more]
AT4G40070.12.7e-2336.48RING/U-box superfamily protein [more]
AT2G18650.17.8e-2332.14RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 110..151
e-value: 1.7E-6
score: 37.6
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 109..152
e-value: 5.8E-12
score: 45.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 110..152
score: 12.40076
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 84..160
e-value: 3.8E-21
score: 76.1
NoneNo IPR availablePANTHERPTHR46539E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 24..406
NoneNo IPR availablePANTHERPTHR46539:SF1E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 24..406
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 109..152
e-value: 3.08448E-19
score: 78.4977
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 89..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003850.1MS003850.1mRNA