MS002886 (gene) Bitter gourd (TR) v1

BLAST of MS002886 vs. NCBI nr
Match: XP_022159348.1 (tRNA (guanine(9)-N1)-methyltransferase-like [Momordica charantia])

HSP 1 Score: 621.3 bits (1601), Expect = 4.7e-174
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60
           MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER
Sbjct: 28  MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 87

Query: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120
           KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL
Sbjct: 88  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 147

Query: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180
           EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK
Sbjct: 148 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 207

Query: 181 EHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240
           EHKSYIE LEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI
Sbjct: 208 EHKSYIETLEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 267

Query: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300
           QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA
Sbjct: 268 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 327

Query: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 328
           VDEENGEKDGEHETKKTKCDEISSSNQ
Sbjct: 328 VDEENGEKDGEHETKKTKCDEISSSNQ 354

BLAST of MS002886 vs. NCBI nr
Match: XP_022159352.1 (tRNA (guanine(9)-N1)-methyltransferase-like [Momordica charantia])

HSP 1 Score: 621.3 bits (1601), Expect = 4.7e-174
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60
           MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER
Sbjct: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60

Query: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120
           KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL
Sbjct: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120

Query: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180
           EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK
Sbjct: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180

Query: 181 EHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240
           EHKSYIE LEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI
Sbjct: 181 EHKSYIETLEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240

Query: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300
           QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA
Sbjct: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300

Query: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 328
           VDEENGEKDGEHETKKTKCDEISSSNQ
Sbjct: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 327

BLAST of MS002886 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 290/330 (87.88%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEA+E D  AKMDP    S C PPE PLSKNARKKL KQQRYEAKKAEKKAQ KE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWEE LASV+EEER+KLIESR+SLRKERMGKRSEE+EKK+ERLNRAKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFS LMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEH+SYI+AL+DQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D AVD EENG++D E ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSN 330

BLAST of MS002886 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.7 bits (1397), Expect = 2.1e-150
Identity = 290/330 (87.88%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEA E D  AKMDP    S C PPE PLSKNARKKL KQQRYEAKKAEKKAQ KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWEE LASV+EEER+KLIESR+SLRKERMGKRSEE+EKK+ERLNRAKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFS LMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EH+SYI+AL+DQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D AVD EENG++D E ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSN 330

BLAST of MS002886 vs. NCBI nr
Match: XP_022973151.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 539.3 bits (1388), Expect = 2.4e-149
Identity = 287/330 (86.97%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEA+E D  AKMDP    S C PPE PLSKNARKKL KQQRYEAKKAEKKAQ KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWE+ LASV+EEER+KLIESRRSLRKERMGKRSEE+EKK+ERLNRAKDCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFS LMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEH+SYI+AL+DQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D AVD EENG++D E E+KK KC E+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSN 330

BLAST of MS002886 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 113/297 (38.05%), Postives = 169/297 (56.90%), Query Frame = 0

Query: 25  LSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIERKRKEWEEMLASVSEEERSKLIESR 84
           LSK+A K+L++QQ ++A + EK+A+ + +KK+               + +EER + IE+ 
Sbjct: 32  LSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKIEAG 91

Query: 85  RSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDLEFSHLMTPSEINSLVQQIMYCYAV 144
             ++           +KK  RL +       IV+D  F  LM   EINSL QQ+  C++ 
Sbjct: 92  EVVKS----------QKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRCHSA 151

Query: 145 NGRCSSPGHLWLTGCNGEMET-QLHRLPG-FDKW-IIEKEHKSYIEALEDQKENLVYLTA 204
           N     P  L+ T   G ++T Q   L G  + W       KSY+E  E QKE LVYL+A
Sbjct: 152 NRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVYLSA 211

Query: 205 DAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTV 264
           D++  + +LD  KIYIIG +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++LTV
Sbjct: 212 DSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKILTV 271

Query: 265 NQVLEILLKYLETRDWKASFFEVVPQRK--RCEADSNSDPAVDEENGEKDGEHETKK 317
           NQV EIL  +LE RDW+ +F EV+P+RK    ++D + D + D  +     + E +K
Sbjct: 272 NQVFEILSLWLEYRDWEKAFMEVIPKRKGILLKSDESFDVSEDTRSQSNQSDSELEK 302

BLAST of MS002886 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-34
Identity = 112/316 (35.44%), Postives = 166/316 (52.53%), Query Frame = 0

Query: 24  PLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIERKRKEWEEMLASVSEEERSKLIES 83
           P+SK   KKL KQ+++E ++     + ++QK+K+ +RKRK+ E      S  + S     
Sbjct: 39  PISKRQMKKLMKQKQWEEQR-----ELRKQKRKE-KRKRKQLERQCQPESNSDGSDRKRI 98

Query: 84  RRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDLEFSHLMTPSEINSLVQQIMYCYA 143
           RR +    +                       ++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RRDVVHSPL----------------------RLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 144 VNGRCSSPGHLWLTGCNGEMETQL-HRLPGFDKW---IIEKEHKSYIEALEDQKENLVYL 203
            N R   P   +LT   G+++  +     G+  W    I+ EH  Y E +  QKE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEH--YSEFI--QKEDLIYL 218

Query: 204 TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 263
           T+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVL 278

Query: 264 TVNQVLEILLKYLETRDWKASFFEVVPQRK-------------------RCEADSNSDPA 317
            VN V EI+L+YLETRDW+ +FF ++PQRK                   R E   NSD +
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACESCSHDKKFARVEGGLNSDSS 322

BLAST of MS002886 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 111/315 (35.24%), Postives = 177/315 (56.19%), Query Frame = 0

Query: 24  PLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIERKRKEWEEMLASVSEEERSKLIES 83
           P+SK   KKL KQ+++E ++     + ++QK+K+ +RKRK+ E                 
Sbjct: 39  PISKRQMKKLIKQKQWEEQR-----ELRKQKRKE-KRKRKKLE----------------- 98

Query: 84  RRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDLEFSHLMTPSEINSLVQQIMYCYA 143
               R+ +M   S+  ++K  R +        ++ID  F HLM   +I  L +QI  CYA
Sbjct: 99  ----RQCQMEPNSDGHDRKRVRRDVVHST-LRLIIDCSFDHLMVLKDIKKLHKQIQRCYA 158

Query: 144 VNGRCSSPGHLWLTGCNGEMETQL-HRLPGFDKW---IIEKEHKSYIEALEDQKENLVYL 203
            N R   P   +LT   G+++  +     G+  W    I+ EH  Y E +  +KE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEH--YSELI--KKEDLIYL 218

Query: 204 TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 263
           T+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDYGINHAQLPLGNFVKMNSRKVL 278

Query: 264 TVNQVLEILLKYLETRDWKASFFEVVPQR-------KRCEADSNSDPAVDEENGEKDGEH 323
            VN V EI+L+YLETRDW+ +FF ++PQR       K CE+ S+ + +V  E G  D + 
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDKACESASHDNQSVRMEEGGSDSDS 321

Query: 324 ETKKTKCDEISSSNQ 328
             ++   +E+ S ++
Sbjct: 339 SEEEYSRNELDSPHE 321

BLAST of MS002886 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.9e-34
Identity = 115/328 (35.06%), Postives = 176/328 (53.66%), Query Frame = 0

Query: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQ---AKEQKKKD 60
           +EAV       +DP       +L  S++A      Q+   A ++E KAQ   +K Q KK 
Sbjct: 3   LEAVTEPGKCSIDP----NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQMKKF 62

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           +  K+K+WE+      ++ + K    +R L ++   + + +   +    +  +     ++
Sbjct: 63  L--KQKQWEDQRELRKQKRKEK--RQKRKLERQAQAEHNIDANSRKRFRHEVQPSALRLI 122

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDK-W 180
           ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ +     +DK W
Sbjct: 123 IDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMDE---YDKGW 182

Query: 181 IIEKEHKSYIEALED--QKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKK 240
           I  K+     E  +D  +KE+LVYLT+D+  VL +LD  K YIIGGLVD N  KGIT KK
Sbjct: 183 INWKDIHIKPEHYKDLIKKEDLVYLTSDSPEVLSELDETKAYIIGGLVDHNHHKGITYKK 242

Query: 241 AQEQGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRK----- 300
           A E GI  A+LP+G+++KM++ +VL VN V EI+L +LE ++WK +FF V+PQRK     
Sbjct: 243 ALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAFFSVLPQRKGAIPL 302

Query: 301 -------RCEA-------DSNSDPAVDE 304
                   C A       DS+SD ++DE
Sbjct: 303 TETGEQPECRASEQEDGEDSDSDSSIDE 319

BLAST of MS002886 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-32
Identity = 102/271 (37.64%), Postives = 157/271 (57.93%), Query Frame = 0

Query: 26  SKNARKKLQKQQRYEAKKAEK-KAQAKEQKKKDIER--KRKEWEEMLASVSEEERSKLIE 85
           S N  +KL      E ++  +  A A+   K+ +++  K+K+WEE      E+ + K   
Sbjct: 12  SPNVEEKLGTSDGEEERQEPRVDAGAEPISKRQLKKLMKQKQWEEQREQRKEKRKEK--R 71

Query: 86  SRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDLEFSHLMTPSEINSLVQQIMYCY 145
            R+ L + ++   S+  ++K  R + A+     +VID  F  LM   +I  L +QI  CY
Sbjct: 72  KRKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCY 131

Query: 146 AVNGRCSSPGHLWLTGCNGEMETQL-HRLPGFDKW---IIEKEHKSYIEALEDQKENLVY 205
           A N R S P   +LT   G+++  +     G+  W    I+ EH  Y E +  +KE+LVY
Sbjct: 132 AENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEH--YSELI--KKEDLVY 191

Query: 206 LTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 265
           LT+D+  VL DLD  K Y+IGGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +V
Sbjct: 192 LTSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKV 251

Query: 266 LTVNQVLEILLKYLETRDWKASFFEVVPQRK 290
           L VN V EI+L++LETRDW+ +FF ++PQRK
Sbjct: 252 LAVNHVFEIILEFLETRDWQEAFFTILPQRK 275

BLAST of MS002886 vs. ExPASy TrEMBL
Match: A0A6J1DZL7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111025770 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60
           MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER
Sbjct: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60

Query: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120
           KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL
Sbjct: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120

Query: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180
           EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK
Sbjct: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180

Query: 181 EHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240
           EHKSYIE LEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI
Sbjct: 181 EHKSYIETLEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240

Query: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300
           QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA
Sbjct: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300

Query: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 328
           VDEENGEKDGEHETKKTKCDEISSSNQ
Sbjct: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 327

BLAST of MS002886 vs. ExPASy TrEMBL
Match: A0A6J1DYJ8 (tRNA (guanine(9)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111025766 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 326/327 (99.69%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 60
           MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER
Sbjct: 28  MEAVENDHGAKMDPLSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIER 87

Query: 61  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 120
           KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL
Sbjct: 88  KRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDL 147

Query: 121 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 180
           EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK
Sbjct: 148 EFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEK 207

Query: 181 EHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 240
           EHKSYIE LEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI
Sbjct: 208 EHKSYIETLEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGI 267

Query: 241 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 300
           QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA
Sbjct: 268 QTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNSDPA 327

Query: 301 VDEENGEKDGEHETKKTKCDEISSSNQ 328
           VDEENGEKDGEHETKKTKCDEISSSNQ
Sbjct: 328 VDEENGEKDGEHETKKTKCDEISSSNQ 354

BLAST of MS002886 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.1e-149
Identity = 287/330 (86.97%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEA+E D  AKMDP    S C PPE PLSKNARKKL KQQRYEAKKAEKKAQ KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWE+ LASV+EEER+KLIESRRSLRKERMGKRSEE+EKK+ERLNRAKDCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFS LMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEH+SYI+AL+DQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D AVD EENG++D E E+KK KC E+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSN 330

BLAST of MS002886 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.3e-149
Identity = 287/330 (86.97%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEA E D  AKMDP    S C PPE PLSKNARKKL KQQRYEAKKAEKKAQ KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWEE LASV+EEER+KLIESR+SLRKERMGKRSEE+EKK+ERLNRAKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFS LMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEH+SYI+AL+D KENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILL+Y+ETRDWKASFF+VVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D AVD +ENG++D E ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSN 330

BLAST of MS002886 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.6e-143
Identity = 280/330 (84.85%), Postives = 301/330 (91.21%), Query Frame = 0

Query: 1   MEAVENDHGAKMDP---LSACQPPELPLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKD 60
           MEAVE  H  KMDP    SAC PPE PLSKNARKKL KQQRYEAKKA+KK QAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIV 120
           IERKRKEWEE LASV+EEER+KLIESRR+LRKERMGKRSE+REKK+ERL+ A+D GQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWI 180
           IDLEFSHLMTP EI+SLVQQIMYCYAVNGRC SP HLWLTGCNGEMETQL RLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHKSYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKE +SYI+AL++QKENLVYLTAD+ETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRCEADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILLKYLETRDWK SFFEVVPQRKRC  DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DPAVD-EENGEKDGEHETKKTKCDEISSSN 327
           D  VD EENG+ D ++ETKKTKCDE+SSS+
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSD 330

BLAST of MS002886 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 391.0 bits (1003), Expect = 9.6e-109
Identity = 220/320 (68.75%), Postives = 258/320 (80.62%), Query Frame = 0

Query: 6   NDHGAKMDPLSACQPPEL--PLSKNARKKLQKQQRYEAKKAEKKAQAKEQKKKDIERKRK 65
           +D+G    P S   P  +  PLSKNA+KK  KQQRYEAKKAEKKAQ KE K+K+ ERK K
Sbjct: 8   DDNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGERKLK 67

Query: 66  EWEEMLASVSEEERSKLIESRRSLRKERMGKRSEEREKKMERLNRAKDCGQNIVIDLEFS 125
           EWEE LA+ +EEER KLIESR+SLRKERM KRSEE+EKK+ERLN+AK+ GQ IV+D++F+
Sbjct: 68  EWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVDVDFA 127

Query: 126 HLMTPSEINSLVQQIMYCYAVNGRCSSPGHLWLTGCNGEMETQLHRLPGFDKWIIEKEHK 185
           HLM+ SEI+SLVQQIMYCYAVNGR +SP HLWLTG  G+M T+L +LPGF+KW IEKE +
Sbjct: 128 HLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIEKESR 187

Query: 186 SYIEALEDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTA 245
            YIEA+ DQK+NLVYLTAD+ETVLDDLD K IYIIGGLVDRNR+KGITM KAQEQGI+TA
Sbjct: 188 CYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQGIKTA 247

Query: 246 KLPIGSYLKMSSSQVLTVNQVLEILLKYLETRDWKASFFEVVPQRKRC---EADSNSDPA 305
           KLPIG Y+KMSSSQVLTVNQVLEIL+K+LETRDWK +FF V+PQRKR      D +    
Sbjct: 248 KLPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLDPVDCSKLEH 307

Query: 306 VDEENGEKDGEHETKKTKCD 321
           + EE+ EKD E      K D
Sbjct: 308 ISEEHQEKDTEANESADKHD 327