MS001708 (gene) Bitter gourd (TR) v1

Overview
NameMS001708
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein-serine/threonine phosphatase
Locationscaffold571: 409842 .. 411142 (+)
RNA-Seq ExpressionMS001708
SyntenyMS001708
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGTATCCATACAAAGTGTTGCTGTCGCTACCCAAAATCGTTGGTCGGAGAATCCCAAGACTTCAGGGAAGGAATTCCTTACTGTCTGCAAGATAAAAACACTCTGATAGAGAGATCATTGGAGTTTGTTCCTGTTTCTTCCCACAACTATCATTTGGAATACTTGGTACTGGCTCAGCGTGGTTTCTACCCAGAATCTCCTGATAAAGAAAACCAAGACAGTTTCTGCATTAGAAGAAATATACAAGGTAACCCAAATGCCCATTTCTTTGGTGTTTTTGATGGGCATGGCCAGTTTGGGATGCAGTGTTCAAATTTTGTTAAGGATAGGCTAATAGAAAAGCTGTGCAATGATCCCACATTGTTAGATGATCCTGTACAAGCTTACAATTCAGCATTTTTATCCACAAATGAGGAGTTGCATACAAGTGAGATTGATGATTCCATGAGTGGAACAACAGCAATTACAGTCCTTGTTGTTGGGGATACCCTCTATGTGGCCAATGTGGGTGATTCAAGGGCTGTAATTGCAGTTAAAGAAGGGAATTGTGTCCTTGCCAAGGACTTGTCAACTGATCAAACTCCATTTAGAAAGGATGAGTATGAGAGAGTGAAGCTTTGTGGGGCTAGAGTTCTGAGTGTTGATCAAGTGGAAGGACTTGTGGATCCTGATATTCAGTGCTGGGGTGATGAAGAAAGTCAGGGTGGCGACCCGCCTCGACTCTGGGTTCAAAATGGGTTGTATCCCGGGACTGCATTTACCAGGAGCATAGGTGATAGTACTGCAGAGAAGATCGGTGTAAACGCTGTTCCCGAGGTCTTAGTTCTTCAGCTTAACCCCAATCATCTTTTCTTTGTCGTCGCTAGCGATGGAGTTTTCGAGTTCCTTTCCAGTCAAACTGTTGTTGATATGGTATGTATTGTGATCAATTTCTAGCATGATTTAGCTAACATCATCATCATTTGATTCATATCTTTGTTTGATGAGTTCTTTTCTAGTTTTGGTCGTGATATCTTGATTTCCCTGTCAAAGTTCTGTAGCTATGAATCACTTGTTAGAGTTACTACTAGTCTCTTGCAAATGACAGCCTATCTTTATCTTCAAATTGTGATACTTTGGGATCTTATCTTAACAAAAATTTCTAATGTGTTTTCTCCATATAGGCATCAAGTTATGCAGATCCTCAAGATGCATGTACTGCTATCGCTGCCGAGTCATACAAACTGTGGATGGAACATGAAAATCGAACCGATGACATAACGATGATTATTGTTCATATCAAAGGCCTGTCTAAT

mRNA sequence

ATGGGTTGTATCCATACAAAGTGTTGCTGTCGCTACCCAAAATCGTTGGTCGGAGAATCCCAAGACTTCAGGGAAGGAATTCCTTACTGTCTGCAAGATAAAAACACTCTGATAGAGAGATCATTGGAGTTTGTTCCTGTTTCTTCCCACAACTATCATTTGGAATACTTGGTACTGGCTCAGCGTGGTTTCTACCCAGAATCTCCTGATAAAGAAAACCAAGACAGTTTCTGCATTAGAAGAAATATACAAGGTAACCCAAATGCCCATTTCTTTGGTGTTTTTGATGGGCATGGCCAGTTTGGGATGCAGTGTTCAAATTTTGTTAAGGATAGGCTAATAGAAAAGCTGTGCAATGATCCCACATTGTTAGATGATCCTGTACAAGCTTACAATTCAGCATTTTTATCCACAAATGAGGAGTTGCATACAAGTGAGATTGATGATTCCATGAGTGGAACAACAGCAATTACAGTCCTTGTTGTTGGGGATACCCTCTATGTGGCCAATGTGGGTGATTCAAGGGCTGTAATTGCAGTTAAAGAAGGGAATTGTGTCCTTGCCAAGGACTTGTCAACTGATCAAACTCCATTTAGAAAGGATGAGTATGAGAGAGTGAAGCTTTGTGGGGCTAGAGTTCTGAGTGTTGATCAAGTGGAAGGACTTGTGGATCCTGATATTCAGTGCTGGGGTGATGAAGAAAGTCAGGGTGGCGACCCGCCTCGACTCTGGGTTCAAAATGGGTTGTATCCCGGGACTGCATTTACCAGGAGCATAGGTGATAGTACTGCAGAGAAGATCGGTGTAAACGCTGTTCCCGAGGTCTTAGTTCTTCAGCTTAACCCCAATCATCTTTTCTTTGTCGTCGCTAGCGATGGAGTTTTCGAGTTCCTTTCCAGTCAAACTGTTGTTGATATGGCATCAAGTTATGCAGATCCTCAAGATGCATGTACTGCTATCGCTGCCGAGTCATACAAACTGTGGATGGAACATGAAAATCGAACCGATGACATAACGATGATTATTGTTCATATCAAAGGCCTGTCTAAT

Coding sequence (CDS)

ATGGGTTGTATCCATACAAAGTGTTGCTGTCGCTACCCAAAATCGTTGGTCGGAGAATCCCAAGACTTCAGGGAAGGAATTCCTTACTGTCTGCAAGATAAAAACACTCTGATAGAGAGATCATTGGAGTTTGTTCCTGTTTCTTCCCACAACTATCATTTGGAATACTTGGTACTGGCTCAGCGTGGTTTCTACCCAGAATCTCCTGATAAAGAAAACCAAGACAGTTTCTGCATTAGAAGAAATATACAAGGTAACCCAAATGCCCATTTCTTTGGTGTTTTTGATGGGCATGGCCAGTTTGGGATGCAGTGTTCAAATTTTGTTAAGGATAGGCTAATAGAAAAGCTGTGCAATGATCCCACATTGTTAGATGATCCTGTACAAGCTTACAATTCAGCATTTTTATCCACAAATGAGGAGTTGCATACAAGTGAGATTGATGATTCCATGAGTGGAACAACAGCAATTACAGTCCTTGTTGTTGGGGATACCCTCTATGTGGCCAATGTGGGTGATTCAAGGGCTGTAATTGCAGTTAAAGAAGGGAATTGTGTCCTTGCCAAGGACTTGTCAACTGATCAAACTCCATTTAGAAAGGATGAGTATGAGAGAGTGAAGCTTTGTGGGGCTAGAGTTCTGAGTGTTGATCAAGTGGAAGGACTTGTGGATCCTGATATTCAGTGCTGGGGTGATGAAGAAAGTCAGGGTGGCGACCCGCCTCGACTCTGGGTTCAAAATGGGTTGTATCCCGGGACTGCATTTACCAGGAGCATAGGTGATAGTACTGCAGAGAAGATCGGTGTAAACGCTGTTCCCGAGGTCTTAGTTCTTCAGCTTAACCCCAATCATCTTTTCTTTGTCGTCGCTAGCGATGGAGTTTTCGAGTTCCTTTCCAGTCAAACTGTTGTTGATATGGCATCAAGTTATGCAGATCCTCAAGATGCATGTACTGCTATCGCTGCCGAGTCATACAAACTGTGGATGGAACATGAAAATCGAACCGATGACATAACGATGATTATTGTTCATATCAAAGGCCTGTCTAAT

Protein sequence

MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLAQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCNDPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAVKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN
Homology
BLAST of MS001708 vs. NCBI nr
Match: XP_022138655.1 (probable protein phosphatase 2C 35 [Momordica charantia] >XP_022138657.1 probable protein phosphatase 2C 35 [Momordica charantia])

HSP 1 Score: 722.2 bits (1863), Expect = 2.1e-204
Identity = 348/350 (99.43%), Postives = 349/350 (99.71%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVL KDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGL+DPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLGKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLLDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 350

BLAST of MS001708 vs. NCBI nr
Match: XP_031743345.1 (probable protein phosphatase 2C 35 isoform X1 [Cucumis sativus] >XP_031743346.1 probable protein phosphatase 2C 35 isoform X1 [Cucumis sativus] >KGN46170.1 hypothetical protein Csa_005534 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 2.7e-199
Identity = 335/350 (95.71%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR NIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. NCBI nr
Match: XP_031743347.1 (probable protein phosphatase 2C 35 isoform X2 [Cucumis sativus] >XP_031743348.1 probable protein phosphatase 2C 35 isoform X2 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 2.7e-199
Identity = 335/350 (95.71%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR NIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. NCBI nr
Match: XP_008458523.1 (PREDICTED: probable protein phosphatase 2C 35 [Cucumis melo] >KAA0033443.1 putative protein phosphatase 2C 35 [Cucumis melo var. makuwa])

HSP 1 Score: 704.9 bits (1818), Expect = 3.5e-199
Identity = 334/350 (95.43%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR N+QGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNVQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. NCBI nr
Match: XP_038874599.1 (probable protein phosphatase 2C 35 isoform X2 [Benincasa hispida] >XP_038874600.1 probable protein phosphatase 2C 35 isoform X2 [Benincasa hispida])

HSP 1 Score: 701.0 bits (1808), Expect = 5.0e-198
Identity = 336/351 (95.73%), Postives = 346/351 (98.58%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDK-NTLIERSLEFVPVSSHNYHLEYLVL 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDK NT+IE+SLE +PV+SHNYHLEYLVL
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNNTVIEKSLELIPVASHNYHLEYLVL 60

Query: 61  AQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN 120
           AQRG+YPESPDKENQDSFCIR NIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN
Sbjct: 61  AQRGYYPESPDKENQDSFCIRTNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN 120

Query: 121 DPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIA 180
           DPTLLDDPVQAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIA
Sbjct: 121 DPTLLDDPVQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIA 180

Query: 181 VKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGD 240
           VKEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGD
Sbjct: 181 VKEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGD 240

Query: 241 PPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLS 300
           PPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLS
Sbjct: 241 PPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLS 300

Query: 301 SQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           SQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 SQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 351

BLAST of MS001708 vs. ExPASy Swiss-Prot
Match: Q7XJ53 (Probable protein phosphatase 2C 35 OS=Arabidopsis thaliana OX=3702 GN=At3g06270 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-139
Identity = 245/351 (69.80%), Postives = 284/351 (80.91%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLV-GESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVL 60
           MGC+  KCC RYP S   G+S+   E     L+ K+   ++ L  + V S N+ + Y VL
Sbjct: 1   MGCVQCKCCSRYPSSSSDGDSRGPLEA-NGVLKGKD---QKPLGSIHVPSPNFDMVYSVL 60

Query: 61  AQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN 120
           +QRG+YP+SPDKENQD++CI+  +QGNPN HFFGVFDGHG  G QCSNFVK+R++E L  
Sbjct: 61  SQRGYYPDSPDKENQDTYCIKTELQGNPNVHFFGVFDGHGVLGTQCSNFVKERVVEMLSE 120

Query: 121 DPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIA 180
           DPTLL+DP +AY SAFL  NEELH SEIDDSMSGTTAITVLVVGD +YVANVGDSRAV+A
Sbjct: 121 DPTLLEDPEKAYKSAFLRVNEELHDSEIDDSMSGTTAITVLVVGDKIYVANVGDSRAVLA 180

Query: 181 VKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGD 240
           VK+ N +LA+DLS DQTPFRKDE ERVK CGARVLSVDQVEGL DP+IQ W +EES+GGD
Sbjct: 181 VKDRNRILAEDLSYDQTPFRKDECERVKACGARVLSVDQVEGLKDPNIQTWANEESEGGD 240

Query: 241 PPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLS 300
           PPRLWVQNG+YPGTAFTRS+GD TAE IGV A PEV ++ L+PNHLFFVVASDG+FEFL 
Sbjct: 241 PPRLWVQNGMYPGTAFTRSVGDFTAESIGVIAEPEVSMVHLSPNHLFFVVASDGIFEFLP 300

Query: 301 SQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           SQ VVDM   YADP+D C A AAESYKLW+EHENRTDDIT+IIV IK LSN
Sbjct: 301 SQAVVDMVGRYADPRDGCAAAAAESYKLWLEHENRTDDITIIIVQIKKLSN 347

BLAST of MS001708 vs. ExPASy Swiss-Prot
Match: Q9SL76 (Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At2g20050/At2g20040 PE=2 SV=2)

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-112
Identity = 198/306 (64.71%), Postives = 235/306 (76.80%), Query Frame = 0

Query: 45  VPVSSHNYHLEYLVLAQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQ 104
           V V S NY L    L+QRG+YP++ DK NQDSF I      N + HFFGVFDGHG+FG Q
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 105 CSNFVKDRLIEKLCNDPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGD 164
           CS FVK RL E L        DP +A NSAFL+TN +LH   +DDSMSGTTAITV+V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 165 TLYVANVGDSRAVIAVKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVD 224
           T+YVAN GDSRAV+A K    ++A DLS DQTPFR DE ERVKLCGARVL++DQ+EGL +
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 225 PDIQCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNH 284
           PD+QCWG EE   GDPPRLWV NG+YPGTAFTRSIGDS AE IGV A PE+ V++L P++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 285 LFFVVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVH 344
            FFVVASDGVFEF+SSQTVVDM + + DP+DAC AI AESY+LW+++E RTDDIT+I+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 345 IKGLSN 351
           I GL +
Sbjct: 397 IDGLKD 402

BLAST of MS001708 vs. ExPASy Swiss-Prot
Match: Q8H2T0 (Probable protein phosphatase 2C 65 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0646100 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 9.1e-94
Identity = 185/362 (51.10%), Postives = 229/362 (63.26%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGC   KCC       V   Q  R G           + R    V V      LEY  LA
Sbjct: 1   MGCAQGKCC-------VPRRQRGRGGGGAVGGRGGATLGR----VAVPGAGLVLEYATLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLC-- 120
             G YP+SP +E+QD+  +     G+P+ H F VFDGHG  G  C+ F +D L   L   
Sbjct: 61  VAGLYPDSPGRESQDAHLVATRFAGHPDLHLFAVFDGHGACGAACAGFARDALPRLLAGV 120

Query: 121 ---------NDPTLLDDPVQAYNSAFLSTNEELHTS-EIDDSMSGTTAITVLVVGDTLYV 180
                        +++DP  A+  A  + N E+H + E+DDSMSGTTA+  LV G  L+V
Sbjct: 121 GVGAGEEGGGRMVVVEDPAAAFREALPAANAEMHAADEVDDSMSGTTAVAALVAGGALHV 180

Query: 181 ANVGDSRAVIAVKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQ 240
           ANVGDSRAV  V     V A++LS DQTPFR DE  RVK CGARV+SV+QVEG+ DP+ +
Sbjct: 181 ANVGDSRAVAGVWREGRVAAEELSWDQTPFRADERARVKACGARVMSVEQVEGVRDPEAE 240

Query: 241 CWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFV 300
            W  +E   GDPPR+W ++GLYPGTAFTRS+GD  AE +GV A PEV  +++ P HLFFV
Sbjct: 241 SWVADE---GDPPRVWARDGLYPGTAFTRSLGDQAAEAVGVIAEPEVKSVEITPAHLFFV 300

Query: 301 VASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGL 351
           VASDGVFEFLSSQ VVDM ++Y DP++AC+AIAAESYKLW+EHENRTDDIT+IIVHI+  
Sbjct: 301 VASDGVFEFLSSQDVVDMVAAYEDPREACSAIAAESYKLWLEHENRTDDITIIIVHIRDS 348

BLAST of MS001708 vs. ExPASy Swiss-Prot
Match: Q8H4S6 (Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0566200 PE=2 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 1.1e-27
Identity = 87/286 (30.42%), Postives = 141/286 (49.30%), Query Frame = 0

Query: 73  NQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN--DPTLLDDPVQA 132
           NQD+  +     G  +    GVFDGHG  G   +  V+D L  +L +    +  D P  A
Sbjct: 66  NQDAMLVWDGFGGEDDGVLCGVFDGHGPHGHVVARRVRDSLPLRLMSAARDSGADMPAAA 125

Query: 133 YNSAFL----STNEELHT-SEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAVKE--G 192
           +  AF     + +++L +   +D   SG+TA+TVL +G  LY+AN+GDSRAV+  +E  G
Sbjct: 126 WRKAFARAYKAMDKDLRSHPSLDCFCSGSTAVTVLKLGSDLYMANIGDSRAVLGSREATG 185

Query: 193 NCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDPPRL 252
             ++A  L+ D  P    E ER+K C  RV ++       +P++             PR+
Sbjct: 186 GGMVAVQLTVDLKPDVPSEAERIKKCRGRVFALQD-----EPEV-------------PRV 245

Query: 253 WVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSSQTV 312
           W+     PG A  R+ GD   +  GV +VPE     L     F ++ASDGV++ LS+Q  
Sbjct: 246 WLPFDDAPGLAMARAFGDFCLKDYGVISVPEFFHWSLTEKDQFVILASDGVWDVLSNQEA 305

Query: 313 VDMASSYADPQDACTAIAAESYKLWMEH--ENRTDDITMIIVHIKG 348
           VD+ S+      A  ++   + + W      ++ DD  ++ +++ G
Sbjct: 306 VDIVSASPSRSKAAKSLVEAATREWKTKYPTSKIDDCAVVCLYLDG 333

BLAST of MS001708 vs. ExPASy Swiss-Prot
Match: Q0WRB2 (Probable protein phosphatase 2C 73 OS=Arabidopsis thaliana OX=3702 GN=PPC6-7 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.8e-26
Identity = 87/305 (28.52%), Postives = 148/305 (48.52%), Query Frame = 0

Query: 73  NQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRL-IEKLCN----------DP 132
           NQD   +        +  F G+FDGHG +G   +  V++ + +  LCN          +P
Sbjct: 74  NQDCALVWEGFGCQEDMIFCGIFDGHGPWGHYVAKQVRNSMPLSLLCNWQKILAQATLEP 133

Query: 133 TL-----------LDDPVQAYNSAFLSTNEEL-HTSEIDDSMSGTTAITVLVVGDTLYVA 192
            L            D   Q+Y     + ++EL H  +ID   SGTTA+T++  G+ +YVA
Sbjct: 134 ELDLEGSNKKISRFDIWKQSYLKTCATVDQELEHHRKIDSYYSGTTALTIVRQGEVIYVA 193

Query: 193 NVGDSRAVIAVK--EGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDI 252
           NVGDSRAV+A++  EG+ V A  L+ D  P    E ER+  C  RV  +D   G+     
Sbjct: 194 NVGDSRAVLAMESDEGSLV-AVQLTLDFKPNLPQEKERIIGCKGRVFCLDDEPGV----- 253

Query: 253 QCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFF 312
                         R+W  +   PG A +R+ GD   ++ G+ +VPEV    ++    F 
Sbjct: 254 -------------HRVWQPDAETPGLAMSRAFGDYCIKEYGLVSVPEVTQRHISTKDHFI 313

Query: 313 VVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHEN--RTDDITMIIVHI 351
           ++ASDG+++ +S+Q  +++ SS A+   A   +  ++ + W +       DD++++ + +
Sbjct: 314 ILASDGIWDVISNQEAIEIVSSTAERPKAAKRLVEQAVRAWKKKRRGYSMDDMSVVCLFL 359

BLAST of MS001708 vs. ExPASy TrEMBL
Match: A0A6J1CA22 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111009760 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.0e-204
Identity = 348/350 (99.43%), Postives = 349/350 (99.71%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVL KDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGL+DPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLGKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLLDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 350

BLAST of MS001708 vs. ExPASy TrEMBL
Match: A0A0A0K8T8 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_6G062300 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 335/350 (95.71%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR NIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. ExPASy TrEMBL
Match: A0A5A7SSL0 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001030 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.7e-199
Identity = 334/350 (95.43%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR N+QGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNVQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. ExPASy TrEMBL
Match: A0A1S3C857 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103497906 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.7e-199
Identity = 334/350 (95.43%), Postives = 346/350 (98.86%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDFR+G+PYCLQDKNT+IE+SLE +PV+SHNYHLEYLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFRQGVPYCLQDKNTVIEKSLEMIPVASHNYHLEYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR N+QGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNVQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDP+QAYNSAFLSTN+ELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPIQAYNSAFLSTNDELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+I+V IKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIVVQIKGLSN 350

BLAST of MS001708 vs. ExPASy TrEMBL
Match: A0A6J1EPW0 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436760 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 4.1e-198
Identity = 331/350 (94.57%), Postives = 345/350 (98.57%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLVGESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVLA 60
           MGCIHTKCCCRYPKSLVGESQDF +G+PYCLQD NT+IER+LE +PV+SHNYHL+YLVLA
Sbjct: 1   MGCIHTKCCCRYPKSLVGESQDFHQGVPYCLQDNNTVIERALELIPVASHNYHLDYLVLA 60

Query: 61  QRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120
           QRG+YPESPDKENQDSFCIR N+QGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND
Sbjct: 61  QRGYYPESPDKENQDSFCIRTNVQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCND 120

Query: 121 PTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180
           PTLLDDPVQAYNSAFLSTN+ELH+SEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV
Sbjct: 121 PTLLDDPVQAYNSAFLSTNDELHSSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIAV 180

Query: 181 KEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240
           KEGNCVLAKDLS DQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP
Sbjct: 181 KEGNCVLAKDLSNDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGDP 240

Query: 241 PRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300
           PRLWVQNGLYPGTAFTRSIGDSTAE+IGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS
Sbjct: 241 PRLWVQNGLYPGTAFTRSIGDSTAEQIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLSS 300

Query: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDIT+II+HIKGLSN
Sbjct: 301 QTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITIIIIHIKGLSN 350

BLAST of MS001708 vs. TAIR 10
Match: AT3G06270.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 7.8e-141
Identity = 245/351 (69.80%), Postives = 284/351 (80.91%), Query Frame = 0

Query: 1   MGCIHTKCCCRYPKSLV-GESQDFREGIPYCLQDKNTLIERSLEFVPVSSHNYHLEYLVL 60
           MGC+  KCC RYP S   G+S+   E     L+ K+   ++ L  + V S N+ + Y VL
Sbjct: 1   MGCVQCKCCSRYPSSSSDGDSRGPLEA-NGVLKGKD---QKPLGSIHVPSPNFDMVYSVL 60

Query: 61  AQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRLIEKLCN 120
           +QRG+YP+SPDKENQD++CI+  +QGNPN HFFGVFDGHG  G QCSNFVK+R++E L  
Sbjct: 61  SQRGYYPDSPDKENQDTYCIKTELQGNPNVHFFGVFDGHGVLGTQCSNFVKERVVEMLSE 120

Query: 121 DPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGDTLYVANVGDSRAVIA 180
           DPTLL+DP +AY SAFL  NEELH SEIDDSMSGTTAITVLVVGD +YVANVGDSRAV+A
Sbjct: 121 DPTLLEDPEKAYKSAFLRVNEELHDSEIDDSMSGTTAITVLVVGDKIYVANVGDSRAVLA 180

Query: 181 VKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDIQCWGDEESQGGD 240
           VK+ N +LA+DLS DQTPFRKDE ERVK CGARVLSVDQVEGL DP+IQ W +EES+GGD
Sbjct: 181 VKDRNRILAEDLSYDQTPFRKDECERVKACGARVLSVDQVEGLKDPNIQTWANEESEGGD 240

Query: 241 PPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFFVVASDGVFEFLS 300
           PPRLWVQNG+YPGTAFTRS+GD TAE IGV A PEV ++ L+PNHLFFVVASDG+FEFL 
Sbjct: 241 PPRLWVQNGMYPGTAFTRSVGDFTAESIGVIAEPEVSMVHLSPNHLFFVVASDGIFEFLP 300

Query: 301 SQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVHIKGLSN 351
           SQ VVDM   YADP+D C A AAESYKLW+EHENRTDDIT+IIV IK LSN
Sbjct: 301 SQAVVDMVGRYADPRDGCAAAAAESYKLWLEHENRTDDITIIIVQIKKLSN 347

BLAST of MS001708 vs. TAIR 10
Match: AT2G20050.1 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 407.9 bits (1047), Expect = 8.1e-114
Identity = 198/306 (64.71%), Postives = 235/306 (76.80%), Query Frame = 0

Query: 45  VPVSSHNYHLEYLVLAQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQ 104
           V V S NY L    L+QRG+YP++ DK NQDSF I      N + HFFGVFDGHG+FG Q
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 105 CSNFVKDRLIEKLCNDPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGD 164
           CS FVK RL E L        DP +A NSAFL+TN +LH   +DDSMSGTTAITV+V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 165 TLYVANVGDSRAVIAVKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVD 224
           T+YVAN GDSRAV+A K    ++A DLS DQTPFR DE ERVKLCGARVL++DQ+EGL +
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 225 PDIQCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNH 284
           PD+QCWG EE   GDPPRLWV NG+YPGTAFTRSIGDS AE IGV A PE+ V++L P++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 285 LFFVVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVH 344
            FFVVASDGVFEF+SSQTVVDM + + DP+DAC AI AESY+LW+++E RTDDIT+I+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 345 IKGLSN 351
           I GL +
Sbjct: 397 IDGLKD 402

BLAST of MS001708 vs. TAIR 10
Match: AT2G20050.2 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 407.9 bits (1047), Expect = 8.1e-114
Identity = 198/306 (64.71%), Postives = 235/306 (76.80%), Query Frame = 0

Query: 45  VPVSSHNYHLEYLVLAQRGFYPESPDKENQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQ 104
           V V S NY L    L+QRG+YP++ DK NQDSF I      N + HFFGVFDGHG+FG Q
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 105 CSNFVKDRLIEKLCNDPTLLDDPVQAYNSAFLSTNEELHTSEIDDSMSGTTAITVLVVGD 164
           CS FVK RL E L        DP +A NSAFL+TN +LH   +DDSMSGTTAITV+V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 165 TLYVANVGDSRAVIAVKEGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVD 224
           T+YVAN GDSRAV+A K    ++A DLS DQTPFR DE ERVKLCGARVL++DQ+EGL +
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 225 PDIQCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNH 284
           PD+QCWG EE   GDPPRLWV NG+YPGTAFTRSIGDS AE IGV A PE+ V++L P++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 285 LFFVVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHENRTDDITMIIVH 344
            FFVVASDGVFEF+SSQTVVDM + + DP+DAC AI AESY+LW+++E RTDDIT+I+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 345 IKGLSN 351
           I GL +
Sbjct: 397 IDGLKD 402

BLAST of MS001708 vs. TAIR 10
Match: AT5G27930.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.0e-27
Identity = 87/305 (28.52%), Postives = 148/305 (48.52%), Query Frame = 0

Query: 73  NQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRL-IEKLCN----------DP 132
           NQD   +        +  F G+FDGHG +G   +  V++ + +  LCN          +P
Sbjct: 74  NQDCALVWEGFGCQEDMIFCGIFDGHGPWGHYVAKQVRNSMPLSLLCNWQKILAQATLEP 133

Query: 133 TL-----------LDDPVQAYNSAFLSTNEEL-HTSEIDDSMSGTTAITVLVVGDTLYVA 192
            L            D   Q+Y     + ++EL H  +ID   SGTTA+T++  G+ +YVA
Sbjct: 134 ELDLEGSNKKISRFDIWKQSYLKTCATVDQELEHHRKIDSYYSGTTALTIVRQGEVIYVA 193

Query: 193 NVGDSRAVIAVK--EGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDI 252
           NVGDSRAV+A++  EG+ V A  L+ D  P    E ER+  C  RV  +D   G+     
Sbjct: 194 NVGDSRAVLAMESDEGSLV-AVQLTLDFKPNLPQEKERIIGCKGRVFCLDDEPGV----- 253

Query: 253 QCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFF 312
                         R+W  +   PG A +R+ GD   ++ G+ +VPEV    ++    F 
Sbjct: 254 -------------HRVWQPDAETPGLAMSRAFGDYCIKEYGLVSVPEVTQRHISTKDHFI 313

Query: 313 VVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHEN--RTDDITMIIVHI 351
           ++ASDG+++ +S+Q  +++ SS A+   A   +  ++ + W +       DD++++ + +
Sbjct: 314 ILASDGIWDVISNQEAIEIVSSTAERPKAAKRLVEQAVRAWKKKRRGYSMDDMSVVCLFL 359

BLAST of MS001708 vs. TAIR 10
Match: AT5G27930.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.0e-27
Identity = 87/305 (28.52%), Postives = 148/305 (48.52%), Query Frame = 0

Query: 73  NQDSFCIRRNIQGNPNAHFFGVFDGHGQFGMQCSNFVKDRL-IEKLCN----------DP 132
           NQD   +        +  F G+FDGHG +G   +  V++ + +  LCN          +P
Sbjct: 74  NQDCALVWEGFGCQEDMIFCGIFDGHGPWGHYVAKQVRNSMPLSLLCNWQKILAQATLEP 133

Query: 133 TL-----------LDDPVQAYNSAFLSTNEEL-HTSEIDDSMSGTTAITVLVVGDTLYVA 192
            L            D   Q+Y     + ++EL H  +ID   SGTTA+T++  G+ +YVA
Sbjct: 134 ELDLEGSNKKISRFDIWKQSYLKTCATVDQELEHHRKIDSYYSGTTALTIVRQGEVIYVA 193

Query: 193 NVGDSRAVIAVK--EGNCVLAKDLSTDQTPFRKDEYERVKLCGARVLSVDQVEGLVDPDI 252
           NVGDSRAV+A++  EG+ V A  L+ D  P    E ER+  C  RV  +D   G+     
Sbjct: 194 NVGDSRAVLAMESDEGSLV-AVQLTLDFKPNLPQEKERIIGCKGRVFCLDDEPGV----- 253

Query: 253 QCWGDEESQGGDPPRLWVQNGLYPGTAFTRSIGDSTAEKIGVNAVPEVLVLQLNPNHLFF 312
                         R+W  +   PG A +R+ GD   ++ G+ +VPEV    ++    F 
Sbjct: 254 -------------HRVWQPDAETPGLAMSRAFGDYCIKEYGLVSVPEVTQRHISTKDHFI 313

Query: 313 VVASDGVFEFLSSQTVVDMASSYADPQDACTAIAAESYKLWMEHEN--RTDDITMIIVHI 351
           ++ASDG+++ +S+Q  +++ SS A+   A   +  ++ + W +       DD++++ + +
Sbjct: 314 ILASDGIWDVISNQEAIEIVSSTAERPKAAKRLVEQAVRAWKKKRRGYSMDDMSVVCLFL 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138655.12.1e-20499.43probable protein phosphatase 2C 35 [Momordica charantia] >XP_022138657.1 probabl... [more]
XP_031743345.12.7e-19995.71probable protein phosphatase 2C 35 isoform X1 [Cucumis sativus] >XP_031743346.1 ... [more]
XP_031743347.12.7e-19995.71probable protein phosphatase 2C 35 isoform X2 [Cucumis sativus] >XP_031743348.1 ... [more]
XP_008458523.13.5e-19995.43PREDICTED: probable protein phosphatase 2C 35 [Cucumis melo] >KAA0033443.1 putat... [more]
XP_038874599.15.0e-19895.73probable protein phosphatase 2C 35 isoform X2 [Benincasa hispida] >XP_038874600.... [more]
Match NameE-valueIdentityDescription
Q7XJ531.1e-13969.80Probable protein phosphatase 2C 35 OS=Arabidopsis thaliana OX=3702 GN=At3g06270 ... [more]
Q9SL761.1e-11264.71Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
Q8H2T09.1e-9451.10Probable protein phosphatase 2C 65 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q8H4S61.1e-2730.42Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q0WRB22.8e-2628.52Probable protein phosphatase 2C 73 OS=Arabidopsis thaliana OX=3702 GN=PPC6-7 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CA221.0e-20499.43Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A0A0K8T81.3e-19995.71Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_6G062300 ... [more]
A0A5A7SSL01.7e-19995.43Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C8571.7e-19995.43Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103497906 PE=... [more]
A0A6J1EPW04.1e-19894.57Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114367... [more]
Match NameE-valueIdentityDescription
AT3G06270.17.8e-14169.80Protein phosphatase 2C family protein [more]
AT2G20050.18.1e-11464.71protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT2G20050.28.1e-11464.71protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT5G27930.12.0e-2728.52Protein phosphatase 2C family protein [more]
AT5G27930.22.0e-2728.52Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 58..343
e-value: 2.9E-55
score: 199.6
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 73..324
e-value: 5.5E-39
score: 134.3
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 55..345
score: 43.797176
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 71..345
e-value: 4.92367E-63
score: 199.475
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 49..347
e-value: 6.4E-68
score: 231.4
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 47..347
NoneNo IPR availablePANTHERPTHR24353CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASEcoord: 37..350
NoneNo IPR availablePANTHERPTHR24353:SF127PROTEIN PHOSPHATASE 2C AND CYCLIC NUCLEOTIDE-BINDING/KINASE DOMAIN-CONTAINING PROTEINcoord: 37..350
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 91..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001708.1MS001708.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity