MS001284 (gene) Bitter gourd (TR) v1

BLAST of MS001284 vs. NCBI nr
Match: XP_022131857.1 (uncharacterized protein LOC111004901 [Momordica charantia])

HSP 1 Score: 856.3 bits (2211), Expect = 1.1e-244
Identity = 420/425 (98.82%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 37  MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 96

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE
Sbjct: 97  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 156

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEI GDLLVGADGCLSSVLQTFLP
Sbjct: 157 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIFGDLLVGADGCLSSVLQTFLP 216

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK
Sbjct: 217 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 276

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRV+KETKEPFINVIYD
Sbjct: 277 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVIKETKEPFINVIYD 336

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQSALAEYQ
Sbjct: 337 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQSALAEYQ 396

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           SIRLPVSSKQVLHSRYMGRLKQGLALPD EPFNPRVATPQDYLELQQKNIPFFNDVPQLI
Sbjct: 397 SIRLPVSSKQVLHSRYMGRLKQGLALPDCEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 456

Query: 421 DPTLK 426
           DPTLK
Sbjct: 457 DPTLK 461

BLAST of MS001284 vs. NCBI nr
Match: KAG7020364.1 (2,6-dihydroxypyridine 3-monooxygenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 765.0 bits (1974), Expect = 3.4e-217
Identity = 373/425 (87.76%), Postives = 395/425 (92.94%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQILEKSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 437 MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILEKSTSPPTGSSTGAGLGLDPLSQS 496

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT+DQNQATD E K RRIL +DENFNFRAAHWADLHGLL+NE
Sbjct: 497 LIQSWLSKPQLLLQSTLPLTVDQNQATDEETKGRRILNRDENFNFRAAHWADLHGLLYNE 556

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLSFCTSEDK+SVKIAA VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 557 LPPDIFLWGHQFLSFCTSEDKSSVKIAANVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 616

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IRKAYP+LGKCLYFDLGSGTHIVLYEL  KK
Sbjct: 617 NFQLRYSGYCAWRGVLDFSENENSETIIDIRKAYPDLGKCLYFDLGSGTHIVLYELPNKK 676

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKV D MVRKM EQAEKVWVPEF RV+KETKEPFINVIYD
Sbjct: 677 INWIWYINQPEPQLKGKSLTMKVDDGMVRKMHEQAEKVWVPEFARVIKETKEPFINVIYD 736

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 737 RNPLERLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 796

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF+P+VA P++  ELQQKNIPFFNDVPQLI
Sbjct: 797 SVRLPVSSKQVLHSRFMGRLKQGLNLPDREPFDPKVAMPENCQELQQKNIPFFNDVPQLI 856

Query: 421 DPTLK 426
           D T K
Sbjct: 857 DTTRK 861

BLAST of MS001284 vs. NCBI nr
Match: KAG6585445.1 (Aurachin C monooxygenase/isomerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 765.0 bits (1974), Expect = 3.4e-217
Identity = 373/425 (87.76%), Postives = 395/425 (92.94%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQILEKSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 1   MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILEKSTSPPTGSSTGAGLGLDPLSQS 60

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT+DQNQATD E K RRIL +DENFNFRAAHWADLHGLL+NE
Sbjct: 61  LIQSWLSKPQLLLQSTLPLTVDQNQATDEETKGRRILNRDENFNFRAAHWADLHGLLYNE 120

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLSFCTSEDK+SVKIAA VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 121 LPPDIFLWGHQFLSFCTSEDKSSVKIAANVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 180

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IRKAYP+LGKCLYFDLGSGTHIVLYEL  KK
Sbjct: 181 NFQLRYSGYCAWRGVLDFSENENSETIIDIRKAYPDLGKCLYFDLGSGTHIVLYELPNKK 240

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKV D MVRKM EQAEKVWVPEF RV+KETKEPFINVIYD
Sbjct: 241 INWIWYINQPEPQLKGKSLTMKVDDGMVRKMHEQAEKVWVPEFARVIKETKEPFINVIYD 300

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 301 RNPLERLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 360

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF+P+VA P++  ELQQKNIPFFNDVPQLI
Sbjct: 361 SVRLPVSSKQVLHSRFMGRLKQGLNLPDREPFDPKVAMPENCQELQQKNIPFFNDVPQLI 420

Query: 421 DPTLK 426
           D T K
Sbjct: 421 DTTRK 425

BLAST of MS001284 vs. NCBI nr
Match: XP_022951855.1 (uncharacterized protein LOC111454587 [Cucurbita moschata])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 370/425 (87.06%), Postives = 396/425 (93.18%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQILEKSTSPPTGSSTGAGLGLD LSQS
Sbjct: 1   MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILEKSTSPPTGSSTGAGLGLDSLSQS 60

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT+DQNQATD E K RRILT+DENFNFRAAHWADLHGLL+NE
Sbjct: 61  LIQSWLSKPQLLLQSTLPLTVDQNQATDEETKGRRILTRDENFNFRAAHWADLHGLLYNE 120

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLSFCTSEDK+SVKIAA+VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 121 LPPDIFLWGHQFLSFCTSEDKSSVKIAARVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 180

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IRKA+P+ GKCLYFDLGSGTHIV+YEL  KK
Sbjct: 181 NFQLRYSGYCAWRGVLDFSENENSETIIDIRKAFPDFGKCLYFDLGSGTHIVIYELPNKK 240

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKV D MVRKM EQAEKVWVPEF RV+KETKEPFINVIYD
Sbjct: 241 INWIWYINQPEPQLKGKSLTMKVDDGMVRKMHEQAEKVWVPEFARVIKETKEPFINVIYD 300

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 301 RNPLERLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 360

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF+P+VA P++  ELQQKNIPFFNDVPQLI
Sbjct: 361 SVRLPVSSKQVLHSRFMGRLKQGLTLPDREPFDPKVAMPENCQELQQKNIPFFNDVPQLI 420

Query: 421 DPTLK 426
           D T+K
Sbjct: 421 DTTMK 425

BLAST of MS001284 vs. NCBI nr
Match: XP_023538143.1 (uncharacterized protein LOC111799001 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023538144.1 uncharacterized protein LOC111799001 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 370/425 (87.06%), Postives = 394/425 (92.71%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQILEKSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 1   MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILEKSTSPPTGSSTGAGLGLDPLSQS 60

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT+DQNQATD E K RRILT+DENFNFRAAHWADLHGLL+NE
Sbjct: 61  LIQSWVSKPQLLLQSTLPLTVDQNQATDEETKGRRILTRDENFNFRAAHWADLHGLLYNE 120

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLSFCTSEDK+SVKIAA+VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 121 LPPDIFLWGHQFLSFCTSEDKSSVKIAARVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 180

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IR+AYP+LGKCLYFDLGSGTHIVLYEL  KK
Sbjct: 181 NFQLRYSGYCAWRGVLDFSENENSETIIDIRRAYPDLGKCLYFDLGSGTHIVLYELPNKK 240

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKV D MVRKM EQAEKVWVPEF RV+KETKEPFINVIYD
Sbjct: 241 INWIWYINQPEPQLKGKSLTMKVDDGMVRKMHEQAEKVWVPEFARVIKETKEPFINVIYD 300

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNV LVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 301 RNPLERLVWDNVALVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 360

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF+P+ A P +  ELQQKNIPFFNDVPQLI
Sbjct: 361 SVRLPVSSKQVLHSRFMGRLKQGLTLPDREPFDPKAAMPDNCQELQQKNIPFFNDVPQLI 420

Query: 421 DPTLK 426
           D T +
Sbjct: 421 DTTTR 425

BLAST of MS001284 vs. ExPASy Swiss-Prot
Match: H1ZZA4 (Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.3e-19
Identity = 107/391 (27.37%), Postives = 162/391 (41.43%), Query Frame = 0

Query: 11  VIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQSWISHPL 70
           +I GG I G++   AL +AG   +I E++   P     GAG+ +            S+ +
Sbjct: 8   LIAGGGIGGLTLGVALRRAGIAFKIFERA---PALLRVGAGISMQ-----------SNAM 67

Query: 71  LLLHSTLPLTIDQNQATDG-EAKARRIL---------------TKDENFNFRAAHWADLH 130
           L   +   L +D   A  G E +   IL               + +        H   L 
Sbjct: 68  LAFRT---LGVDTAVAAAGQEIQGGAILNPRGEEISSMPVSKASAEVGAPMITIHRGRLQ 127

Query: 131 GLLHNELPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSV 190
            +LH  +  D  + G     F    D   V++A       +  E  GDLLVGADG  S+V
Sbjct: 128 DVLHQIVGDDNLVLGAKVEGFRDGPDGLFVRLA-------DGREFQGDLLVGADGLRSAV 187

Query: 191 LQTFLPNLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLY 250
               L     RYSGY +WRGV D SE        G+R+ Y           G G    + 
Sbjct: 188 RAQLLKEPSPRYSGYTSWRGVCDVSE--------GVRRDYTS------ESWGPGMRFGVV 247

Query: 251 ELLKKKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPF 310
            + + +  W      PE    G         E++++        W     ++++ T    
Sbjct: 248 PIGEGQTYWFATATAPE----GGVDHPDARTELLQRFSG-----WHAPIPQLIENTPSSA 307

Query: 311 I--NVIYDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLE-H 370
           I    I+DR P+ Q V    VL+GDAAHP TP+  +    ++ DA VL +CL    LE  
Sbjct: 308 IMRTDIHDRVPIRQWVQGRAVLLGDAAHPMTPNMGQGGCQAVEDAVVLARCLS---LEAE 348

Query: 371 LQSALAEYQSIRLPVSSKQVLHSRYMGRLKQ 383
           L +ALA YQ++R+  ++  V  S  +G++ Q
Sbjct: 368 LPAALARYQAVRVERANDFVAGSYRIGQIGQ 348

BLAST of MS001284 vs. ExPASy Swiss-Prot
Match: Q93NG3 (2,6-dihydroxypyridine 3-monooxygenase OS=Paenarthrobacter nicotinovorans OX=29320 GN=dhpH PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 3.9e-14
Identity = 99/416 (23.80%), Postives = 186/416 (44.71%), Query Frame = 0

Query: 9   RAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDP-LSQSLIQSWIS 68
           R  +VGGSI+G+++A  L  AG +V + E+S  P +G   G G+ + P L   L++  + 
Sbjct: 7   RIAVVGGSISGLTAALMLRDAGVDVDVYERSPQPLSG--FGTGIVVQPELVHYLLEQGVE 66

Query: 69  HPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNELPPDIFL 128
              + + S+   +++   A  GE    R+ +   ++ F +  +  ++G L+    P+   
Sbjct: 67  LDSISVPSS---SMEYVDALTGE----RVGSVPADWRFTS--YDSIYGGLYELFGPE--- 126

Query: 129 WGHHFLSFC---TSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNLKL 188
              +  S C    S+D  +V+     ++  +  +   + ++GADG  +SV++  L  ++ 
Sbjct: 127 --RYHTSKCLVGLSQDSETVQ-----MRFSDGTKAEANWVIGADGG-ASVVRKRLLGIEP 186

Query: 189 RYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKK----- 248
            Y+GY  WRGVL   E  D          +        + L    H++ Y +  +     
Sbjct: 187 TYAGYVTWRGVLQPGEVADD--------VWNYFNDKFTYGLLDDGHLIAYPIPGRENAES 246

Query: 249 -KINWIWYINQPE--------PQLKGNSLTMKVHDEM-----VRKMQEQAEKVWVPEFVR 308
            ++N+ WY N  E          ++G  L   VH+       +R+   + E ++ P F  
Sbjct: 247 PRLNFQWYWNVAEGPDLDELMTDVRGIRLPTSVHNNSLNPHNLRQFHSKGESLFKP-FRD 306

Query: 309 VVKETKEPFINVIYDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLE 368
           +V     PF+ V+ D   ++++V   V+L+GDAA    PH       +  DA  L +   
Sbjct: 307 LVLNASSPFVTVVADA-TVDRMVHGRVLLIGDAAVTPRPHAAAGGAKACDDARTLAEVFT 366

Query: 369 KWRLEHLQSALAEYQSIRLPVSSKQVLHSRYMGRLKQGLALPDH----EPFNPRVA 398
           K     L+ +L  +++ +L     Q  H+ Y+ ++K+  +   H    EP NP  A
Sbjct: 367 K--NHDLRGSLQSWETRQL-----QQGHA-YLNKVKKMASRLQHGGSFEPGNPAFA 382

BLAST of MS001284 vs. ExPASy Swiss-Prot
Match: Q88FY2 (6-hydroxynicotinate 3-monooxygenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=nicC PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.1e-14
Identity = 81/361 (22.44%), Postives = 149/361 (41.27%), Query Frame = 0

Query: 7   RPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQSWI 66
           R +  IVG  + G ++A  L +AG++V++ E++   P  +  GAG+ + P    + +   
Sbjct: 4   RQKIAIVGAGLGGAAAATLLQQAGFDVEVFEQA---PAFTRLGAGIHIGPNVMKIFRRMG 63

Query: 67  SHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAA----HWADLHGLLHNELP 126
               L L  + P          G+  +R  L +     + AA    H  DLH L    + 
Sbjct: 64  LEQKLELMGSHPDFWFSRDGNTGDYLSRIPLGEFARREYGAAYITIHRGDLHALQIEAIQ 123

Query: 127 PDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNL 186
           P    +G          D+  +  A       +    V D+++GADG  S + +  L   
Sbjct: 124 PGTVHFGKRLEKIVDEGDQVRLDFA-------DGTHTVADIVIGADGIHSKIREELLGAE 183

Query: 187 KLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKKIN 246
              YSG+ A R ++           + + +       C+ +      H+++Y    K+  
Sbjct: 184 APIYSGWVAHRALI---------RGVNLAQHADVFEPCVKW-WSEDRHMMVYYTTGKRDE 243

Query: 247 WIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYDRD 306
           + +    P          +    E +R     A + + P   +++  T+      + +R+
Sbjct: 244 YYFVTGVPHEAWDFQGAFVDSSQEEMR----AAFEGYHPTVQKLIDATESITKWPLRNRN 303

Query: 307 PLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQSI 364
           PL       +VL+GDA HP  PH  +   M+I DAA+L +CL++  L   ++A A Y++ 
Sbjct: 304 PLPLWSRGRLVLLGDACHPMKPHMAQGACMAIEDAAMLTRCLQETGLSDHRTAFALYEAN 340

BLAST of MS001284 vs. ExPASy Swiss-Prot
Match: Q5EXK1 (3-hydroxybenzoate 6-hydroxylase OS=Klebsiella oxytoca OX=571 GN=mhbM PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-13
Identity = 97/379 (25.59%), Postives = 168/379 (44.33%), Query Frame = 0

Query: 9   RAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQSWISH 68
           RA+IVGG I G ++A +L + G +V +LEK+         GAG+ L P + S + S    
Sbjct: 6   RAIIVGGGIGGAATALSLARQGIKVMLLEKAHEI---GEIGAGIQLGPNAFSALDSLGVG 65

Query: 69  PLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNELPPDIFLW 128
            +    +     I    A +GE     ++  +    FR  H+   + ++H  +     +W
Sbjct: 66  EVARQRAVFTDHITMMDAVNGE----EVVHIETGQAFR-DHFGGPYAVIH-RVDIHATVW 125

Query: 129 ----GHHFLSFCTSEDKASVKIAAK--VLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNL 188
                H  + + TS     ++  A    +  D+      D+L+G DG  S V Q+ L + 
Sbjct: 126 EAALTHPAVEYRTSTQVVDIRQTADDVTVFDDKGNSWTADILIGCDGGKSVVRQSLLGD- 185

Query: 189 KLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELL-KKKI 248
             R +G+  +R V+D ++  D      +R   P L        G   H+V Y L   K+ 
Sbjct: 186 SPRVTGHVVYRAVVDAADMPDD-----LRINAPVLW------AGPHCHLVHYPLRGGKQY 245

Query: 249 NWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYDR 308
           N +   +  + +  G      V D    ++    + +       + K T     +   DR
Sbjct: 246 NLVVTFHSRQQEEWG------VRDGSKEEVLSYFKGIHPRPRQMLDKPTSWRRWSTA-DR 305

Query: 309 DPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQS 368
           +P+E+   D + LVGDAAHP   +  +   M++ DA  LGK L +   +  + A A Y+S
Sbjct: 306 EPVEKWGNDRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCDGDAAR-AFALYES 355

Query: 369 IRLPVSSKQVLHSRYMGRL 381
           +R+P +++ V  +R MGR+
Sbjct: 366 VRIPRTARIVWSTREMGRV 355

BLAST of MS001284 vs. ExPASy Swiss-Prot
Match: Q3S4B7 (3-hydroxybenzoate 6-hydroxylase OS=Polaromonas naphthalenivorans (strain CJ2) OX=365044 GN=nagX PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.4e-11
Identity = 94/382 (24.61%), Postives = 162/382 (42.41%), Query Frame = 0

Query: 11  VIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQSWISHPL 70
           ++ GG I G+++A AL++ G+ V++LE++   P     GAG+ L P +     +      
Sbjct: 11  LVAGGGIGGLAAALALVRRGFSVKVLEQA---PEIGEIGAGIQLGPNAFHAFDALGIGEK 70

Query: 71  LLLHSTLPLTIDQNQATDGEAKARRILTKDENFN------FRAAHWADLH-GLLH--NEL 130
               +     +  + A DG    R  +   E F       +   H  D+H  LL    E 
Sbjct: 71  ARGRAVYTDEMVMHDAIDGSLVGR--IPTGEAFRQRFGNPYAVIHRVDVHLSLLEGAQET 130

Query: 131 PPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPN 190
               FL     L     +D+ SV +  +     +     G  L+GADG  S V + F+ +
Sbjct: 131 GKVEFLTSTRALRI--EQDEGSVTVYDQHGNAHK-----GIALIGADGVKSVVREQFVGD 190

Query: 191 LKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPE--LGKCLYFDLGSGTHIVLYELL-K 250
              R +G+  +R V+D             +K +PE          +G   H+V Y L   
Sbjct: 191 -AARVTGHVVYRAVVD-------------KKDFPESLQWNAASIWVGPNCHLVHYPLRGG 250

Query: 251 KKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVI 310
           ++ N +   +  +P+  G      V +    ++Q   + +  P+  +++   K       
Sbjct: 251 EQYNVVVTFHSRQPEQWG------VTEGSKEEVQSYFQGI-CPQARQLIDLPKTWKRWAT 310

Query: 311 YDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAE 370
            DR+P+ Q  +  V L+GDAAHPTT +  +   M++ D   LG+ L +        A   
Sbjct: 311 ADREPIGQWSFGRVTLLGDAAHPTTQYMAQGACMAMEDGVTLGEAL-RVNNNDFPKAFEL 358

Query: 371 YQSIRLPVSSKQVLHSRYMGRL 381
           YQ  R+  +++ VL SR MGR+
Sbjct: 371 YQRSRVARTARIVLSSREMGRI 358

BLAST of MS001284 vs. ExPASy TrEMBL
Match: A0A6J1BS69 (uncharacterized protein LOC111004901 OS=Momordica charantia OX=3673 GN=LOC111004901 PE=4 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 5.5e-245
Identity = 420/425 (98.82%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 37  MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 96

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE
Sbjct: 97  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 156

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEI GDLLVGADGCLSSVLQTFLP
Sbjct: 157 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIFGDLLVGADGCLSSVLQTFLP 216

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK
Sbjct: 217 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 276

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRV+KETKEPFINVIYD
Sbjct: 277 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVIKETKEPFINVIYD 336

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQSALAEYQ
Sbjct: 337 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQSALAEYQ 396

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           SIRLPVSSKQVLHSRYMGRLKQGLALPD EPFNPRVATPQDYLELQQKNIPFFNDVPQLI
Sbjct: 397 SIRLPVSSKQVLHSRYMGRLKQGLALPDCEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 456

Query: 421 DPTLK 426
           DPTLK
Sbjct: 457 DPTLK 461

BLAST of MS001284 vs. ExPASy TrEMBL
Match: A0A6J1GK26 (uncharacterized protein LOC111454587 OS=Cucurbita moschata OX=3662 GN=LOC111454587 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.3e-217
Identity = 370/425 (87.06%), Postives = 396/425 (93.18%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQILEKSTSPPTGSSTGAGLGLD LSQS
Sbjct: 1   MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILEKSTSPPTGSSTGAGLGLDSLSQS 60

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT+DQNQATD E K RRILT+DENFNFRAAHWADLHGLL+NE
Sbjct: 61  LIQSWLSKPQLLLQSTLPLTVDQNQATDEETKGRRILTRDENFNFRAAHWADLHGLLYNE 120

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLSFCTSEDK+SVKIAA+VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 121 LPPDIFLWGHQFLSFCTSEDKSSVKIAARVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 180

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IRKA+P+ GKCLYFDLGSGTHIV+YEL  KK
Sbjct: 181 NFQLRYSGYCAWRGVLDFSENENSETIIDIRKAFPDFGKCLYFDLGSGTHIVIYELPNKK 240

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKV D MVRKM EQAEKVWVPEF RV+KETKEPFINVIYD
Sbjct: 241 INWIWYINQPEPQLKGKSLTMKVDDGMVRKMHEQAEKVWVPEFARVIKETKEPFINVIYD 300

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 301 RNPLERLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 360

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQLI 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF+P+VA P++  ELQQKNIPFFNDVPQLI
Sbjct: 361 SVRLPVSSKQVLHSRFMGRLKQGLTLPDREPFDPKVAMPENCQELQQKNIPFFNDVPQLI 420

Query: 421 DPTLK 426
           D T+K
Sbjct: 421 DTTMK 425

BLAST of MS001284 vs. ExPASy TrEMBL
Match: A0A6J1KTB5 (uncharacterized protein LOC111496218 OS=Cucurbita maxima OX=3661 GN=LOC111496218 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.1e-216
Identity = 371/426 (87.09%), Postives = 394/426 (92.49%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           MN K R P+AVIVGGSIAGIS AHALIKAGW+VQIL+KSTSPPTGSSTGAGLGLDPLSQS
Sbjct: 1   MNGKNRIPKAVIVGGSIAGISCAHALIKAGWKVQILKKSTSPPTGSSTGAGLGLDPLSQS 60

Query: 61  LIQSWISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNE 120
           LIQSW+S P LLL STLPLT DQNQATD E K RRILT+DENFNFRAAHWADLHGLL+NE
Sbjct: 61  LIQSWLSKPQLLLQSTLPLTFDQNQATDEETKGRRILTRDENFNFRAAHWADLHGLLYNE 120

Query: 121 LPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLP 180
           LPPDIFLWGH FLS+CTSEDK+SVKIAA+VLQTDEI+EIVGDLLVGADGCLSSVLQTFLP
Sbjct: 121 LPPDIFLWGHQFLSYCTSEDKSSVKIAARVLQTDEIIEIVGDLLVGADGCLSSVLQTFLP 180

Query: 181 NLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKK 240
           N +LRYSGYCAWRGVLDFSENE+SETII IRKAYP+LGKCLYFDLGSGTHIVLYEL  KK
Sbjct: 181 NFQLRYSGYCAWRGVLDFSENENSETIIDIRKAYPDLGKCLYFDLGSGTHIVLYELPNKK 240

Query: 241 INWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYD 300
           INWIWYINQPEPQLKG SLTMKVHDEMVRKM EQAEKVWVPEF  V+KETKEPFINVIYD
Sbjct: 241 INWIWYINQPEPQLKGKSLTMKVHDEMVRKMHEQAEKVWVPEFASVIKETKEPFINVIYD 300

Query: 301 RDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQ 360
           R+PLE+LVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW LE+LQ+ALAEYQ
Sbjct: 301 RNPLERLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGLENLQTALAEYQ 360

Query: 361 SIRLPVSSKQVLHSRYMGRLKQGLALPDHEPF-NPRVATPQDYLELQQKNIPFFNDVPQL 420
           S+RLPVSSKQVLHSR+MGRLKQGL LPD EPF +P+   P +  ELQQKNIPFFNDVPQL
Sbjct: 361 SVRLPVSSKQVLHSRFMGRLKQGLTLPDREPFDDPKADMPDNCQELQQKNIPFFNDVPQL 420

Query: 421 IDPTLK 426
           ID T K
Sbjct: 421 IDTTRK 426

BLAST of MS001284 vs. ExPASy TrEMBL
Match: A0A5A7V9F2 (2,6-dihydroxypyridine 3-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002830 PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.3e-209
Identity = 363/427 (85.01%), Postives = 396/427 (92.74%), Query Frame = 0

Query: 1   MNEKKRR-PRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQ 60
           M+E +RR P+A+IVGGSIAGIS AHAL++AGW+VQ+LEKST+PPTGSSTGAGLGLD LSQ
Sbjct: 1   MDENERRIPKALIVGGSIAGISCAHALLEAGWKVQVLEKSTTPPTGSSTGAGLGLDLLSQ 60

Query: 61  SLIQSWISHPLLLLHSTLPLTIDQNQAT-DGEAKARRILTKDENFNFRAAHWADLHGLLH 120
           SLIQSWIS P LLL STLPLT+DQNQAT D E K R IL++DENFNFRAA WADLHGLL+
Sbjct: 61  SLIQSWISDPQLLLQSTLPLTVDQNQATVDRETKERVILSRDENFNFRAALWADLHGLLY 120

Query: 121 NELPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTF 180
           NELPP+IFLWGHHFLSFC SEDKA VKI A+VLQ+ EIVEIVGDLLVGADGCLSSVL TF
Sbjct: 121 NELPPEIFLWGHHFLSFCKSEDKACVKIRARVLQSGEIVEIVGDLLVGADGCLSSVLHTF 180

Query: 181 LPNLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLK 240
           LPN + RYSGYCAWRGVLDFS+NE+SETI+GIR+AYPELGKCLYFDLGS THIVLYEL K
Sbjct: 181 LPNFESRYSGYCAWRGVLDFSKNENSETIVGIRRAYPELGKCLYFDLGSTTHIVLYELPK 240

Query: 241 KKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVI 300
           KK+NWIWYINQPEPQLKGNS+TM+V +EMVRKM EQAEKVWVPEFV+VVKETKEPFINVI
Sbjct: 241 KKLNWIWYINQPEPQLKGNSMTMRVQEEMVRKMHEQAEKVWVPEFVKVVKETKEPFINVI 300

Query: 301 YDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAE 360
           YDR+ LEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW  E+LQSALAE
Sbjct: 301 YDRNSLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGPENLQSALAE 360

Query: 361 YQSIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQ 420
           YQS+RLPVSSKQVLHSRY+GRLKQGLALPD EPF+PRVATP++ LELQQKNIPFFNDVPQ
Sbjct: 361 YQSVRLPVSSKQVLHSRYLGRLKQGLALPDQEPFDPRVATPENCLELQQKNIPFFNDVPQ 420

Query: 421 LIDPTLK 426
           LID T K
Sbjct: 421 LIDTTRK 427

BLAST of MS001284 vs. ExPASy TrEMBL
Match: A0A1S3BCR1 (LOW QUALITY PROTEIN: 2,6-dihydroxypyridine 3-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103488270 PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.3e-209
Identity = 360/427 (84.31%), Postives = 396/427 (92.74%), Query Frame = 0

Query: 1   MNEKKRR-PRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQ 60
           M+E +RR P+A+IVGGSIAGIS AHAL++AGW+VQ+LEKST+PPTGSSTGAGLGLD LSQ
Sbjct: 1   MDENERRIPKALIVGGSIAGISCAHALLEAGWKVQVLEKSTTPPTGSSTGAGLGLDLLSQ 60

Query: 61  SLIQSWISHPLLLLHSTLPLTIDQNQAT-DGEAKARRILTKDENFNFRAAHWADLHGLLH 120
           SLIQSWIS P LLL STLPLT+DQNQAT D E K R IL++DENFNFRAA WADLHGLL+
Sbjct: 61  SLIQSWISDPQLLLQSTLPLTVDQNQATVDRETKERLILSRDENFNFRAALWADLHGLLY 120

Query: 121 NELPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTF 180
           NELP +IFLWGHHFLSFC SEDKA VKI A+VLQ+ EI EIVGDLLVGADGCLSSVL+TF
Sbjct: 121 NELPSEIFLWGHHFLSFCKSEDKACVKIRARVLQSGEIAEIVGDLLVGADGCLSSVLRTF 180

Query: 181 LPNLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLK 240
           LPN +LRYSGYCAWRGVLDFS+NE+SETI+G+R+AYPELGKCLYFDLGS THIVLYEL K
Sbjct: 181 LPNFELRYSGYCAWRGVLDFSKNENSETIVGLRRAYPELGKCLYFDLGSATHIVLYELXK 240

Query: 241 KKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVI 300
           +K+NWIWYINQPEPQLKGNS+TM+V +EMVRKM EQAEKVWVPEFV+VVKETKEPFINVI
Sbjct: 241 EKLNWIWYINQPEPQLKGNSMTMRVQEEMVRKMHEQAEKVWVPEFVKVVKETKEPFINVI 300

Query: 301 YDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAE 360
           YDR+ LEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKW  E+LQSALAE
Sbjct: 301 YDRNSLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWGPENLQSALAE 360

Query: 361 YQSIRLPVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVPQ 420
           YQS+RLPVSSKQVLHSRY+GRLKQGLALPD EPF+PRVATP++ LELQQKNIPFFNDVPQ
Sbjct: 361 YQSVRLPVSSKQVLHSRYLGRLKQGLALPDQEPFDPRVATPENCLELQQKNIPFFNDVPQ 420

Query: 421 LIDPTLK 426
           LID T K
Sbjct: 421 LIDTTRK 427

BLAST of MS001284 vs. TAIR 10
Match: AT5G11330.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 542.0 bits (1395), Expect = 4.4e-154
Identity = 255/413 (61.74%), Postives = 322/413 (77.97%), Query Frame = 0

Query: 5   KRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQS 64
           K++ +A+IVGGSIAG+S AH+L  A W+V +LEKS+ PP GS TGAGLGLDP ++ +I+S
Sbjct: 3   KKKGKAIIVGGSIAGLSCAHSLTLACWDVLVLEKSSEPPVGSPTGAGLGLDPQARQIIKS 62

Query: 65  WISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNELPPD 124
           W++ P LL   TLPL+IDQNQ TD E K  RILT+DE+F+FRAA+W+D+  LL+N LP  
Sbjct: 63  WLTTPQLLDEITLPLSIDQNQTTDSEKKVTRILTRDEDFDFRAAYWSDIQSLLYNALPQT 122

Query: 125 IFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNLKL 184
           +FLWGH FLSF  S+D+++VK+   V++T E VEI GDLL+ ADGCLSS+ +TFLP+ KL
Sbjct: 123 MFLWGHKFLSFTMSQDESNVKVKTLVVETQETVEIEGDLLIAADGCLSSIRKTFLPDFKL 182

Query: 185 RYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKKINWI 244
           RYSGYCAWRGV DFS NE+SET+ GI+K YP+LGKCLYFDLG  TH V YEL  KK+NWI
Sbjct: 183 RYSGYCAWRGVFDFSGNENSETVSGIKKVYPDLGKCLYFDLGEQTHSVFYELFNKKLNWI 242

Query: 245 WYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYDRDPL 304
           WY+NQPEP LK NS+T+KV  EM+ KM ++AE +W+PE  R++ ETK+PF+NVIYD DPL
Sbjct: 243 WYVNQPEPDLKSNSVTLKVSQEMINKMHQEAETIWIPELARLMSETKDPFLNVIYDSDPL 302

Query: 305 EQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQSIRL 364
           E++ W N+VLVGDAAHPTTPHGLRSTNMSILDA VLGKCLE    E++   L EYQ IRL
Sbjct: 303 ERIFWGNIVLVGDAAHPTTPHGLRSTNMSILDAEVLGKCLENCGPENVSLGLEEYQRIRL 362

Query: 365 PVSSKQVLHSRYMGRLKQGLALPDHEPFNPRVATPQDYLELQQKNIPFFNDVP 418
           PV S+QVL++R +GR+KQGL   DH+     V        L+Q+N+PFF+  P
Sbjct: 363 PVVSEQVLYARRLGRIKQGL---DHDGIGDGV------FGLEQRNMPFFSCAP 406

BLAST of MS001284 vs. TAIR 10
Match: AT5G11330.2 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 495.0 bits (1273), Expect = 6.2e-140
Identity = 228/357 (63.87%), Postives = 285/357 (79.83%), Query Frame = 0

Query: 5   KRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQS 64
           K++ +A+IVGGSIAG+S AH+L  A W+V +LEKS+ PP GS TGAGLGLDP ++ +I+S
Sbjct: 3   KKKGKAIIVGGSIAGLSCAHSLTLACWDVLVLEKSSEPPVGSPTGAGLGLDPQARQIIKS 62

Query: 65  WISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNELPPD 124
           W++ P LL   TLPL+IDQNQ TD E K  RILT+DE+F+FRAA+W+D+  LL+N LP  
Sbjct: 63  WLTTPQLLDEITLPLSIDQNQTTDSEKKVTRILTRDEDFDFRAAYWSDIQSLLYNALPQT 122

Query: 125 IFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNLKL 184
           +FLWGH FLSF  S+D+++VK+   V++T E VEI GDLL+ ADGCLSS+ +TFLP+ KL
Sbjct: 123 MFLWGHKFLSFTMSQDESNVKVKTLVVETQETVEIEGDLLIAADGCLSSIRKTFLPDFKL 182

Query: 185 RYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKKINWI 244
           RYSGYCAWRGV DFS NE+SET+ GI+K YP+LGKCLYFDLG  TH V YEL  KK+NWI
Sbjct: 183 RYSGYCAWRGVFDFSGNENSETVSGIKKVYPDLGKCLYFDLGEQTHSVFYELFNKKLNWI 242

Query: 245 WYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYDRDPL 304
           WY+NQPEP LK NS+T+KV  EM+ KM ++AE +W+PE  R++ ETK+PF+NVIYD DPL
Sbjct: 243 WYVNQPEPDLKSNSVTLKVSQEMINKMHQEAETIWIPELARLMSETKDPFLNVIYDSDPL 302

Query: 305 EQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLEKWRLEHLQSALAEYQS 362
           E++ W N+VLVGDAAHPTTPHGLRSTNMSILDA VLGKCLE    E   +A   YQ+
Sbjct: 303 ERIFWGNIVLVGDAAHPTTPHGLRSTNMSILDAEVLGKCLENCGPE---NAFGAYQA 356

BLAST of MS001284 vs. TAIR 10
Match: AT2G29720.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.2e-13
Identity = 88/392 (22.45%), Postives = 158/392 (40.31%), Query Frame = 0

Query: 6   RRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQSLIQSW 65
           R  + VIVGG I G+++A AL + G    +LE++ S  TG   GA L L      ++ + 
Sbjct: 41  REEKVVIVGGGIGGLATAVALHRLGIRSVVLEQAESLRTG---GASLTLSKNGWRVLDAI 100

Query: 66  ISHPLLLLHSTLPLTIDQNQATDGEAKARRILTKDENFNFRAAHWADLHGLLHNELPPDI 125
              P L         +   +    E ++ +    D++   R      L   L ++LPP  
Sbjct: 101 SIGPQLRKQFLEMQGVVLKKEDGRELRSFKFKDNDQSQEVRGVERRVLLETLASQLPPQT 160

Query: 126 FLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGADGCLSSVLQTFLPNLKLR 185
             +     S  ++ +         +LQ  +    + ++++G DG  S V  T++   + +
Sbjct: 161 IRFSSKLESIQSNAN------GDTLLQLKDGTRFLANIVIGCDGIRSKV-ATWMGFSEPK 220

Query: 186 YSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYFDLGSGTHIVLYELLKKKINWIW 245
           Y GYCA+RG+  F   +  +  +              +  G G       +   K+ W  
Sbjct: 221 YVGYCAFRGLGFFPNGQPFQQKVN-------------YIFGRGLRAGYVPVSATKVYWFI 280

Query: 246 YINQPE--PQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFVRVVKETKEPFINVIYDRDP 305
             N P   PQ+   ++  K   E+V          W  +   ++  T +  I+    R P
Sbjct: 281 TFNSPSLGPQMMDPAILRKEAKELV--------STWPEDLQNLIDLTPDEAIS----RTP 340

Query: 306 L-EQLVW---------DNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGKCLE---KWRLE 365
           L ++ +W           VVLVGDA HP TP+  +    ++ D+ +L   L        E
Sbjct: 341 LADRWLWPGIAPSASKGRVVLVGDAWHPMTPNLGQGACCALEDSVLLANKLASAINGGTE 397

Query: 366 HLQSALAEYQSIRLPVSSKQVLHSRYMGRLKQ 383
            ++ A+  Y+S R     +  + +  +G+L Q
Sbjct: 401 SVEGAMESYRSERWSQVFRLTVLANLVGKLLQ 397

BLAST of MS001284 vs. TAIR 10
Match: AT5G67030.1 (zeaxanthin epoxidase (ZEP) (ABA1) )

HSP 1 Score: 62.8 bits (151), Expect = 7.8e-10
Identity = 94/367 (25.61%), Postives = 146/367 (39.78%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           + EKK++ R ++ GG I G+  A A  K G++V + EK  S   G   G   G   +  +
Sbjct: 74  VTEKKKKSRVLVAGGGIGGLVFALAAKKKGFDVLVFEKDLSAIRGE--GKYRGPIQIQSN 133

Query: 61  LIQSWISHPLLLLHSTLP---LTIDQ-NQATDGEAKARRILTKDENFN---------FRA 120
            + +  +  + +    +    +T D+ N   DG +    +  K + F           R 
Sbjct: 134 ALAALEAIDIEVAEQVMEAGCITGDRINGLVDGISGTWYV--KFDTFTPAASRGLPVTRV 193

Query: 121 AHWADLHGLLHNELPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGA 180
                L  +L   +  D+     + + F  S DK +V     VL+  +  E  GDLLVGA
Sbjct: 194 ISRMTLQQILARAVGEDVIRNESNVVDFEDSGDKVTV-----VLENGQRYE--GDLLVGA 253

Query: 181 DGCLSSVLQTFLPNLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYF---D 240
           DG  S V        +  YSGY  + G+ DF    D E+ +G R     LG   YF   D
Sbjct: 254 DGIWSKVRNNLFGRSEATYSGYTCYTGIADFIP-ADIES-VGYRVF---LGHKQYFVSSD 313

Query: 241 LGSGTHIVLYELLKKKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFV 300
           +G G                WY    EP    ++         ++K   +    W    +
Sbjct: 314 VGGG-------------KMQWYAFHEEPAGGADA------PNGMKKRLFEIFDGWCDNVL 373

Query: 301 RVVKETKEPFI--NVIYDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGK 349
            ++  T+E  I    IYDR P        V L+GD+ H   P+  +   M+I D+  L  
Sbjct: 374 DLLHATEEEAILRRDIYDRSPGFTWGKGRVTLLGDSIHAMQPNMGQGGCMAIEDSFQLAL 405

BLAST of MS001284 vs. TAIR 10
Match: AT5G67030.2 (zeaxanthin epoxidase (ZEP) (ABA1) )

HSP 1 Score: 62.8 bits (151), Expect = 7.8e-10
Identity = 94/367 (25.61%), Postives = 146/367 (39.78%), Query Frame = 0

Query: 1   MNEKKRRPRAVIVGGSIAGISSAHALIKAGWEVQILEKSTSPPTGSSTGAGLGLDPLSQS 60
           + EKK++ R ++ GG I G+  A A  K G++V + EK  S   G   G   G   +  +
Sbjct: 74  VTEKKKKSRVLVAGGGIGGLVFALAAKKKGFDVLVFEKDLSAIRGE--GKYRGPIQIQSN 133

Query: 61  LIQSWISHPLLLLHSTLP---LTIDQ-NQATDGEAKARRILTKDENFN---------FRA 120
            + +  +  + +    +    +T D+ N   DG +    +  K + F           R 
Sbjct: 134 ALAALEAIDIEVAEQVMEAGCITGDRINGLVDGISGTWYV--KFDTFTPAASRGLPVTRV 193

Query: 121 AHWADLHGLLHNELPPDIFLWGHHFLSFCTSEDKASVKIAAKVLQTDEIVEIVGDLLVGA 180
                L  +L   +  D+     + + F  S DK +V     VL+  +  E  GDLLVGA
Sbjct: 194 ISRMTLQQILARAVGEDVIRNESNVVDFEDSGDKVTV-----VLENGQRYE--GDLLVGA 253

Query: 181 DGCLSSVLQTFLPNLKLRYSGYCAWRGVLDFSENEDSETIIGIRKAYPELGKCLYF---D 240
           DG  S V        +  YSGY  + G+ DF    D E+ +G R     LG   YF   D
Sbjct: 254 DGIWSKVRNNLFGRSEATYSGYTCYTGIADFIP-ADIES-VGYRVF---LGHKQYFVSSD 313

Query: 241 LGSGTHIVLYELLKKKINWIWYINQPEPQLKGNSLTMKVHDEMVRKMQEQAEKVWVPEFV 300
           +G G                WY    EP    ++         ++K   +    W    +
Sbjct: 314 VGGG-------------KMQWYAFHEEPAGGADA------PNGMKKRLFEIFDGWCDNVL 373

Query: 301 RVVKETKEPFI--NVIYDRDPLEQLVWDNVVLVGDAAHPTTPHGLRSTNMSILDAAVLGK 349
            ++  T+E  I    IYDR P        V L+GD+ H   P+  +   M+I D+  L  
Sbjct: 374 DLLHATEEEAILRRDIYDRSPGFTWGKGRVTLLGDSIHAMQPNMGQGGCMAIEDSFQLAL 405