MS001232 (gene) Bitter gourd (TR) v1

BLAST of MS001232 vs. NCBI nr
Match: XP_022132320.1 (tRNA (guanine-N(7)-)-methyltransferase [Momordica charantia] >XP_022132322.1 tRNA (guanine-N(7)-)-methyltransferase [Momordica charantia])

HSP 1 Score: 512.7 bits (1319), Expect = 1.8e-141
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
           PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG
Sbjct: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           FRAVYRRISAAP
Sbjct: 241 FRAVYRRISAAP 252

BLAST of MS001232 vs. NCBI nr
Match: XP_023536944.1 (tRNA (guanine-N(7)-)-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.1 bits (1250), Expect = 1.8e-133
Identity = 237/252 (94.05%), Postives = 245/252 (97.22%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             RSK++EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRSKRIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. NCBI nr
Match: KAG6585402.1 (tRNA (guanine-N(7)-)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 485.7 bits (1249), Expect = 2.4e-133
Identity = 237/252 (94.05%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             RSKK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LR G
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRAG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. NCBI nr
Match: KAG7020318.1 (tRNA (guanine-N(7)-)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 485.7 bits (1249), Expect = 2.4e-133
Identity = 237/252 (94.05%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 22  MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 81

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             RSKK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 82  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 141

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LR G
Sbjct: 142 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRAG 201

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 202 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 261

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 262 FQAVYRRISAAP 273

BLAST of MS001232 vs. NCBI nr
Match: XP_008445050.1 (PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo] >XP_016899935.1 PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo] >XP_016899936.1 PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo] >XP_016899937.1 PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo] >XP_016899938.1 PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo] >KAA0065020.1 tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 485.3 bits (1248), Expect = 3.1e-133
Identity = 238/252 (94.44%), Postives = 247/252 (98.02%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
            D++KK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QNISV RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK CLENHPLFEALT+EELEADPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. ExPASy Swiss-Prot
Match: Q8GXB7 (tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24840 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 2.7e-111
Identity = 199/242 (82.23%), Postives = 220/242 (90.91%), Query Frame = 0

Query: 9   TISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQPDRSKKVE 68
           T SKSTGLPRKRFYRARAHSNPLSDSHFP+PISP+ VDYSLH+P++  ++++    KKVE
Sbjct: 9   TFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK--FIKKVE 68

Query: 69  FADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILAL-RVANPGQYQNISVVR 128
           FADIGCGFGGLLISL+ LFP+TLMIGMELRDKVTEYVKERILAL R ++ GQY+NISVVR
Sbjct: 69  FADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVVR 128

Query: 129 TNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGIIYTIT 188
           TNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS HLLDEYA+ LR GGIIYTIT
Sbjct: 129 TNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTIT 188

Query: 189 DVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRAVYRR 248
           DVEELGEWMK+CLE HP+FE+LT EEL +DP VELL SATEEGQKVARN GQTFRAV+RR
Sbjct: 189 DVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFRR 248

Query: 249 IS 250
           I+
Sbjct: 249 IA 248

BLAST of MS001232 vs. ExPASy Swiss-Prot
Match: A2YB34 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22311 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 5.2e-107
Identity = 187/239 (78.24%), Postives = 209/239 (87.45%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQPDRS-----KKVEFA 75
           LPRKRFYRARAHSNPLSDSHFP+PISP EVD S HYP++FPS +   R       ++ FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYA+ + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRAVYRRIS 250
           ELGEWM++CLE HPLFEA+  EE +ADP V+LLS+ATEEGQKVARN GQTF+A++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of MS001232 vs. ExPASy Swiss-Prot
Match: Q67V78 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0236900 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 5.2e-107
Identity = 187/239 (78.24%), Postives = 209/239 (87.45%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQPDRS-----KKVEFA 75
           LPRKRFYRARAHSNPLSDSHFP+PISP EVD S HYP++FPS +   R       ++ FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYA+ + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRAVYRRIS 250
           ELGEWM++CLE HPLFEA+  EE +ADP V+LLS+ATEEGQKVARN GQTF+A++RRIS
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of MS001232 vs. ExPASy Swiss-Prot
Match: B6SHG7 (tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 8.3e-105
Identity = 185/239 (77.41%), Postives = 207/239 (86.61%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQPD-----RSKKVEFA 75
           LPRKRFYRARAHSNPLSDSHFPVPISP EVD S HYP++FP++   D       +++ FA
Sbjct: 13  LPRKRFYRARAHSNPLSDSHFPVPISPEEVDLSQHYPRYFPADKGSDGEEAAAPQQIRFA 72

Query: 76  DIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LS LFP+TLMIGMELRDKVTEYVKERILALR ANPGQY NISVVRTNS
Sbjct: 73  DVGCGFGGLLVGLSPLFPDTLMIGMELRDKVTEYVKERILALRGANPGQYDNISVVRTNS 132

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGIIYTITDVE 195
           MKYIPNYF KAQL+KMFFLFPDPHFKEKNHRRRVIS  LLDEYA+ + VGGIIYTITDVE
Sbjct: 133 MKYIPNYFRKAQLTKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 192

Query: 196 ELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRAVYRRIS 250
           ELGEWM++CLE HPLFE +  EE++ADP  +LLS+ATEE QKVARN GQTF A++RRIS
Sbjct: 193 ELGEWMRSCLEKHPLFETVPEEEIKADPVFKLLSTATEESQKVARNGGQTFYAIFRRIS 251

BLAST of MS001232 vs. ExPASy Swiss-Prot
Match: Q6NU94 (tRNA (guanine-N(7)-)-methyltransferase OS=Xenopus laevis OX=8355 GN=mettl1 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.1e-76
Identity = 137/245 (55.92%), Postives = 188/245 (76.73%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWF----------PSNDQPDRSK 75
           LP+KR+YR RAHSNP++D  F  P+ P  +D+S +YP++F           + D  +R +
Sbjct: 17  LPQKRYYRQRAHSNPMADHTFQYPVKPEVMDWSEYYPEFFKPLVPDCAHDDAKDLQERKE 76

Query: 76  --KVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQYQNI 135
             +VEFAD+GCG+GGLL++LS LFP TLM+G+E+R KV++YV++RI +LR ++ GQYQNI
Sbjct: 77  QHQVEFADVGCGYGGLLVALSPLFPNTLMLGLEIRVKVSDYVQDRIKSLRASHLGQYQNI 136

Query: 136 SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGII 195
           + +R+N+MKY+PN+F+K QLSKMFFLFPDPHFK+  H+ R+ISP LL EY++ALRVGG++
Sbjct: 137 ACIRSNAMKYLPNFFKKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYSYALRVGGMV 196

Query: 196 YTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRA 249
           YTITDVEE+ EWM      HPLFE +  EEL +D  V+ L ++TEEG+KV RN+GQ F A
Sbjct: 197 YTITDVEEVHEWMVKHFTEHPLFERVEKEELVSDIIVDKLGTSTEEGKKVQRNKGQNFLA 256

BLAST of MS001232 vs. ExPASy TrEMBL
Match: A0A6J1BTJ1 (tRNA (guanine-N(7)-)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005201 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 8.8e-142
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
           PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG
Sbjct: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           FRAVYRRISAAP
Sbjct: 241 FRAVYRRISAAP 252

BLAST of MS001232 vs. ExPASy TrEMBL
Match: A0A1S4DVC5 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103488206 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.5e-133
Identity = 238/252 (94.44%), Postives = 247/252 (98.02%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
            D++KK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QNISV RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK CLENHPLFEALT+EELEADPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. ExPASy TrEMBL
Match: A0A5A7VCU7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003450 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.5e-133
Identity = 238/252 (94.44%), Postives = 247/252 (98.02%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLE++ANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLENEANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
            D++KK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  RDKNKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QNISV RTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ALRVG
Sbjct: 121 QNISVDRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYALRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK CLENHPLFEALT+EELEADPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKTCLENHPLFEALTSEELEADPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. ExPASy TrEMBL
Match: A0A6J1KTN7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496303 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.6e-133
Identity = 236/252 (93.65%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             R KK+EFADIGCGFGGLLISLS +FPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRPKKIEFADIGCGFGGLLISLSTIFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. ExPASy TrEMBL
Match: A0A6J1GGH7 (tRNA (guanine-N(7)-)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454015 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 7.5e-133
Identity = 236/252 (93.65%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MLEHDANPTISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQ 60
           ML+HDAN TISKSTGLPRKRFYRARAHSNPLSDSHFP+PI PSEVDYSLHYPQ FPSNDQ
Sbjct: 1   MLDHDANSTISKSTGLPRKRFYRARAHSNPLSDSHFPIPICPSEVDYSLHYPQLFPSNDQ 60

Query: 61  PDRSKKVEFADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120
             RSKK+EFADIGCGFGGLLISLS LFPETLMIGMELRDKVTEYVKERILALRVANPGQY
Sbjct: 61  LHRSKKIEFADIGCGFGGLLISLSTLFPETLMIGMELRDKVTEYVKERILALRVANPGQY 120

Query: 121 QNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVG 180
           QN+SVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYA+ LRVG
Sbjct: 121 QNVSVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAYTLRVG 180

Query: 181 GIIYTITDVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQT 240
           GIIYTITDVEELGEWMK+CLENHPLFEALTAEELE DPAV+LLSSATEEGQKVARNEGQT
Sbjct: 181 GIIYTITDVEELGEWMKSCLENHPLFEALTAEELEVDPAVKLLSSATEEGQKVARNEGQT 240

Query: 241 FRAVYRRISAAP 253
           F+AVYRRISAAP
Sbjct: 241 FQAVYRRISAAP 252

BLAST of MS001232 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 402.9 bits (1034), Expect = 1.9e-112
Identity = 199/242 (82.23%), Postives = 220/242 (90.91%), Query Frame = 0

Query: 9   TISKSTGLPRKRFYRARAHSNPLSDSHFPVPISPSEVDYSLHYPQWFPSNDQPDRSKKVE 68
           T SKSTGLPRKRFYRARAHSNPLSDSHFP+PISP+ VDYSLH+P++  ++++    KKVE
Sbjct: 9   TFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK--FIKKVE 68

Query: 69  FADIGCGFGGLLISLSNLFPETLMIGMELRDKVTEYVKERILAL-RVANPGQYQNISVVR 128
           FADIGCGFGGLLISL+ LFP+TLMIGMELRDKVTEYVKERILAL R ++ GQY+NISVVR
Sbjct: 69  FADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISVVR 128

Query: 129 TNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALRVGGIIYTIT 188
           TNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS HLLDEYA+ LR GGIIYTIT
Sbjct: 129 TNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTIT 188

Query: 189 DVEELGEWMKACLENHPLFEALTAEELEADPAVELLSSATEEGQKVARNEGQTFRAVYRR 248
           DVEELGEWMK+CLE HP+FE+LT EEL +DP VELL SATEEGQKVARN GQTFRAV+RR
Sbjct: 189 DVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQTFRAVFRR 248

Query: 249 IS 250
           I+
Sbjct: 249 IA 248

BLAST of MS001232 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-08
Identity = 55/203 (27.09%), Postives = 89/203 (43.84%), Query Frame = 0

Query: 3   EHDANPTISKSTGLPRKRFYRARAHSNPLSDSHF-PVPISPSEVDYSLHYPQWFPSNDQP 62
           E + N  IS+  G       R R H NPLS S   P P+           P W      P
Sbjct: 80  ELNLNHKISEEVG-----HVRIRQHVNPLSSSFSKPAPV-----------PVWDEVYKDP 139

Query: 63  DRSKKVEFADIGCGFGGLLISLSNLFPETL-MIGMELRDKVTEYVKERILALRVANPGQY 122
                V   DIG G G  L+  +N   E+   +G+E+R K+ +     +  L ++     
Sbjct: 140 SLPLMV---DIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS----- 199

Query: 123 QNISVVRTNSMKYIPNYFEK--AQLSKMFFLFPDPHFKEKNHRRRVISPHLLDEYAFALR 182
            N+  +  N+M    +        L  +  L PDPHFK+++ +RRV+   L++     L+
Sbjct: 200 -NVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLK 257

Query: 183 VGGIIYTITDVEELGEWMKACLE 202
            GG I+  +DV ++ + M+  L+
Sbjct: 260 PGGKIFVQSDVLDVAQDMRDQLD 257