MS001068 (gene) Bitter gourd (TR) v1

BLAST of MS001068 vs. NCBI nr
Match: XP_022150402.1 (uncharacterized protein LOC111018568 [Momordica charantia] >XP_022150403.1 uncharacterized protein LOC111018568 [Momordica charantia] >XP_022150404.1 uncharacterized protein LOC111018568 [Momordica charantia] >XP_022150405.1 uncharacterized protein LOC111018568 [Momordica charantia])

HSP 1 Score: 590.9 bits (1522), Expect = 6.2e-165
Identity = 276/298 (92.62%), Postives = 285/298 (95.64%), Query Frame = 0

Query: 1   LNNCRNFSSQKSLVSPCRNTFAGNRPGCNLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKE 60
           LNNCRNFSSQ+S VS CRNTFAGNRPGCNLNS SLRFE   YHSVLQSSSFQHWFKNW+E
Sbjct: 8   LNNCRNFSSQESSVSRCRNTFAGNRPGCNLNSHSLRFETGNYHSVLQSSSFQHWFKNWQE 67

Query: 61  LKRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLIT 120
           L++HKLTASTFAGAIGFWPRRRA+LWLEKLGA EQF G LAT WSN+KE EALERYKLIT
Sbjct: 68  LRKHKLTASTFAGAIGFWPRRRAQLWLEKLGAIEQFSGTLATCWSNNKEEEALERYKLIT 127

Query: 121 GNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPW 180
           GNTVLFPEFQVYGK NSEDDWLAASPDGAIDKIV+GLPSRGVLEIKCPFFDGDMRKASPW
Sbjct: 128 GNTVLFPEFQVYGKVNSEDDWLAASPDGAIDKIVHGLPSRGVLEIKCPFFDGDMRKASPW 187

Query: 181 SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHV 240
           SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYW+VLKMALSDFWWKHV
Sbjct: 188 SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWEVLKMALSDFWWKHV 247

Query: 241 QPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           QPARE CSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKL+LREFDGKLQT
Sbjct: 248 QPAREFCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLILREFDGKLQT 305

BLAST of MS001068 vs. NCBI nr
Match: XP_022132090.1 (uncharacterized protein LOC111005048 [Momordica charantia])

HSP 1 Score: 551.2 bits (1419), Expect = 5.4e-153
Identity = 258/260 (99.23%), Postives = 258/260 (99.23%), Query Frame = 0

Query: 39  MAKYHSVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG 98
           MAKYHSVLQSSSFQHWFKNWKELK HKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG
Sbjct: 1   MAKYHSVLQSSSFQHWFKNWKELKTHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG 60

Query: 99  NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 158
           NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP
Sbjct: 61  NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 120

Query: 159 SRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 218
           SRGVLEIKCPFFDGDMRKASPW RVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL
Sbjct: 121 SRGVLEIKCPFFDGDMRKASPWXRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 180

Query: 219 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 278
           YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES
Sbjct: 181 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 240

Query: 279 KRIVDNSKLLLREFDGKLQT 299
           KRIVDNSKLLLREFDGKLQT
Sbjct: 241 KRIVDNSKLLLREFDGKLQT 260

BLAST of MS001068 vs. NCBI nr
Match: XP_038900094.1 (uncharacterized protein LOC120087241 [Benincasa hispida] >XP_038900095.1 uncharacterized protein LOC120087241 [Benincasa hispida])

HSP 1 Score: 517.7 bits (1332), Expect = 6.7e-143
Identity = 242/290 (83.45%), Postives = 261/290 (90.00%), Query Frame = 0

Query: 17  CRNTFA-----GNRPGCNLN----SQSLRFEMAKYHSVLQSSSFQHWFKNWKELKRHKLT 76
           C+  FA     GN    N N    S +L+FE   ++S+LQSSSFQHWFKNW EL++HKLT
Sbjct: 4   CKKIFACSQAIGNWSIRNFNSAASSSALKFETGNHYSILQSSSFQHWFKNWNELRKHKLT 63

Query: 77  ASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLITGNTVLFP 136
           ASTFAGAIGFWPRRRA+LWLEKLGA +QFCGNLAT WSN KE EALERYKLITGN+VLFP
Sbjct: 64  ASTFAGAIGFWPRRRAQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSVLFP 123

Query: 137 EFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWSRVPLYS 196
           EFQVYGKANS DDWLAASPDGAIDK++YGLPSRGVLEIKCPFFDGDMRKASPWSR+PLY 
Sbjct: 124 EFQVYGKANSVDDWLAASPDGAIDKMIYGLPSRGVLEIKCPFFDGDMRKASPWSRIPLYC 183

Query: 197 IPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQPARELC 256
           IPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYW VLK+ALSDFWWKHVQPARE+C
Sbjct: 184 IPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAREMC 243

Query: 257 SKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQ 298
           SKYAI NPLIELKSLRPSP+HELCSYIVCESKR+VDNSKLLLREFDG+LQ
Sbjct: 244 SKYAINNPLIELKSLRPSPKHELCSYIVCESKRVVDNSKLLLREFDGRLQ 293

BLAST of MS001068 vs. NCBI nr
Match: XP_022981568.1 (uncharacterized protein LOC111480647 [Cucurbita maxima])

HSP 1 Score: 515.8 bits (1327), Expect = 2.5e-142
Identity = 240/296 (81.08%), Postives = 263/296 (88.85%), Query Frame = 0

Query: 3   NCRNFSSQKSLVSPCRNTFAGNRPGCNLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKELK 62
           NC+N  ++   +   RN    N    + +S S +FE   Y+SVLQSSSFQHWFKNWKEL+
Sbjct: 3   NCKNIFARTQTI---RNWSRCNFNSASSSSSSFKFETGNYYSVLQSSSFQHWFKNWKELR 62

Query: 63  RHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLITGN 122
           +HKLTASTFAGAIGFWPRRRA+LWLEKLGA +QF GNLAT WSN KE EALERYKLITGN
Sbjct: 63  KHKLTASTFAGAIGFWPRRRAQLWLEKLGAIDQFSGNLATCWSNMKEEEALERYKLITGN 122

Query: 123 TVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWSR 182
           +VLFPEFQVYGK NSE DWLAASPDGAIDK+VYGLPSRGVLEIKCPFFDGDM KASPWSR
Sbjct: 123 SVLFPEFQVYGKGNSEYDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFDGDMEKASPWSR 182

Query: 183 VPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQP 242
           VPLY IPQ QGLMEIMDRDWMDFYVWTPKGSSLFRLYRD EYW+VLK+ALSDFWWKHVQP
Sbjct: 183 VPLYCIPQVQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDAEYWEVLKIALSDFWWKHVQP 242

Query: 243 ARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           ARE+CSKY+ITNPLIELKSLRPSP+HELCSYIVCESKR+VDNS+LLLREF+G+LQT
Sbjct: 243 AREMCSKYSITNPLIELKSLRPSPKHELCSYIVCESKRVVDNSELLLREFNGRLQT 295

BLAST of MS001068 vs. NCBI nr
Match: XP_011659114.2 (uncharacterized protein LOC101215512 [Cucumis sativus] >KAE8646186.1 hypothetical protein Csa_015911 [Cucumis sativus])

HSP 1 Score: 513.1 bits (1320), Expect = 1.6e-141
Identity = 232/270 (85.93%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 29  NLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWPRRRAELWLE 88
           +++S SL+FE   ++SVLQS+SFQHWFKNW+EL++HKLTASTFAGAIGFWPRRR +LWLE
Sbjct: 25  SVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRVQLWLE 84

Query: 89  KLGATEQFCGNLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDG 148
           KLGA +QFCGNLAT WSN KE EALERYKLITGN+VLFPEFQVYGKANSEDDWLAASPDG
Sbjct: 85  KLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDG 144

Query: 149 AIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVW 208
           AIDK++YGLPS+GVLEIKCPFF+GDMR ASPWSRVPLY IPQAQGLMEIMDRDWMDFYVW
Sbjct: 145 AIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVW 204

Query: 209 TPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRH 268
           TP GSSLFRLYRDVEYW VLK+ALSDFWWKHVQPARE+CSKY +TNPLIELKSLRPSPRH
Sbjct: 205 TPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLIELKSLRPSPRH 264

Query: 269 ELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           ELCSYIVCES+R+V+NSKLLLREFDG+LQT
Sbjct: 265 ELCSYIVCESRRVVNNSKLLLREFDGRLQT 294

BLAST of MS001068 vs. ExPASy TrEMBL
Match: A0A6J1D9D0 (uncharacterized protein LOC111018568 OS=Momordica charantia OX=3673 GN=LOC111018568 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.0e-165
Identity = 276/298 (92.62%), Postives = 285/298 (95.64%), Query Frame = 0

Query: 1   LNNCRNFSSQKSLVSPCRNTFAGNRPGCNLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKE 60
           LNNCRNFSSQ+S VS CRNTFAGNRPGCNLNS SLRFE   YHSVLQSSSFQHWFKNW+E
Sbjct: 8   LNNCRNFSSQESSVSRCRNTFAGNRPGCNLNSHSLRFETGNYHSVLQSSSFQHWFKNWQE 67

Query: 61  LKRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLIT 120
           L++HKLTASTFAGAIGFWPRRRA+LWLEKLGA EQF G LAT WSN+KE EALERYKLIT
Sbjct: 68  LRKHKLTASTFAGAIGFWPRRRAQLWLEKLGAIEQFSGTLATCWSNNKEEEALERYKLIT 127

Query: 121 GNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPW 180
           GNTVLFPEFQVYGK NSEDDWLAASPDGAIDKIV+GLPSRGVLEIKCPFFDGDMRKASPW
Sbjct: 128 GNTVLFPEFQVYGKVNSEDDWLAASPDGAIDKIVHGLPSRGVLEIKCPFFDGDMRKASPW 187

Query: 181 SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHV 240
           SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYW+VLKMALSDFWWKHV
Sbjct: 188 SRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWEVLKMALSDFWWKHV 247

Query: 241 QPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           QPARE CSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKL+LREFDGKLQT
Sbjct: 248 QPAREFCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLILREFDGKLQT 305

BLAST of MS001068 vs. ExPASy TrEMBL
Match: A0A6J1BRH1 (uncharacterized protein LOC111005048 OS=Momordica charantia OX=3673 GN=LOC111005048 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.6e-153
Identity = 258/260 (99.23%), Postives = 258/260 (99.23%), Query Frame = 0

Query: 39  MAKYHSVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG 98
           MAKYHSVLQSSSFQHWFKNWKELK HKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG
Sbjct: 1   MAKYHSVLQSSSFQHWFKNWKELKTHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCG 60

Query: 99  NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 158
           NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP
Sbjct: 61  NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 120

Query: 159 SRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 218
           SRGVLEIKCPFFDGDMRKASPW RVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL
Sbjct: 121 SRGVLEIKCPFFDGDMRKASPWXRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 180

Query: 219 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 278
           YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES
Sbjct: 181 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 240

Query: 279 KRIVDNSKLLLREFDGKLQT 299
           KRIVDNSKLLLREFDGKLQT
Sbjct: 241 KRIVDNSKLLLREFDGKLQT 260

BLAST of MS001068 vs. ExPASy TrEMBL
Match: A0A0A0K4T5 (YqaJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G302370 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 9.4e-143
Identity = 235/270 (87.04%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 29  NLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWPRRRAELWLE 88
           +++S SL+FE   ++SVLQS+SFQHWFKNW+EL++HKLTASTFAGAIGFWPRRR +LWLE
Sbjct: 33  SVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLE 92

Query: 89  KLGATEQFCGNLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDG 148
           KLGA +QFCGNLAT WSN KE EALERYKLITGN+VLFPEFQVYGKANSEDDWLAASPDG
Sbjct: 93  KLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDG 152

Query: 149 AIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVW 208
           AIDK+VYGLPSRGVLEIKCPFF+GDMR ASPWSRVPLY IPQAQGLMEIMDRDWMDFYVW
Sbjct: 153 AIDKMVYGLPSRGVLEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVW 212

Query: 209 TPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRH 268
           TP GSSLFRLYRDVEYW VLK+ALSDFWWKHVQPARE+CSKY +TNPLIELKSLRPSPRH
Sbjct: 213 TPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLIELKSLRPSPRH 272

Query: 269 ELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           ELCSYIVCESKR+V+NSKLLLREFDG+LQT
Sbjct: 273 ELCSYIVCESKRVVNNSKLLLREFDGRLQT 302

BLAST of MS001068 vs. ExPASy TrEMBL
Match: A0A6J1J2F9 (uncharacterized protein LOC111480647 OS=Cucurbita maxima OX=3661 GN=LOC111480647 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.2e-142
Identity = 240/296 (81.08%), Postives = 263/296 (88.85%), Query Frame = 0

Query: 3   NCRNFSSQKSLVSPCRNTFAGNRPGCNLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKELK 62
           NC+N  ++   +   RN    N    + +S S +FE   Y+SVLQSSSFQHWFKNWKEL+
Sbjct: 3   NCKNIFARTQTI---RNWSRCNFNSASSSSSSFKFETGNYYSVLQSSSFQHWFKNWKELR 62

Query: 63  RHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLITGN 122
           +HKLTASTFAGAIGFWPRRRA+LWLEKLGA +QF GNLAT WSN KE EALERYKLITGN
Sbjct: 63  KHKLTASTFAGAIGFWPRRRAQLWLEKLGAIDQFSGNLATCWSNMKEEEALERYKLITGN 122

Query: 123 TVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWSR 182
           +VLFPEFQVYGK NSE DWLAASPDGAIDK+VYGLPSRGVLEIKCPFFDGDM KASPWSR
Sbjct: 123 SVLFPEFQVYGKGNSEYDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFDGDMEKASPWSR 182

Query: 183 VPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQP 242
           VPLY IPQ QGLMEIMDRDWMDFYVWTPKGSSLFRLYRD EYW+VLK+ALSDFWWKHVQP
Sbjct: 183 VPLYCIPQVQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDAEYWEVLKIALSDFWWKHVQP 242

Query: 243 ARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           ARE+CSKY+ITNPLIELKSLRPSP+HELCSYIVCESKR+VDNS+LLLREF+G+LQT
Sbjct: 243 AREMCSKYSITNPLIELKSLRPSPKHELCSYIVCESKRVVDNSELLLREFNGRLQT 295

BLAST of MS001068 vs. ExPASy TrEMBL
Match: A0A6J1EKA9 (uncharacterized protein LOC111433377 OS=Cucurbita moschata OX=3662 GN=LOC111433377 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.0e-141
Identity = 240/297 (80.81%), Postives = 265/297 (89.23%), Query Frame = 0

Query: 3   NCRN-FSSQKSLVSPCRNTFAGNRPGCNLNSQSLRFEMAKYHSVLQSSSFQHWFKNWKEL 62
           NC+N F+  +++ +  R  F  N    + +S S +FE   Y+SVLQSSSFQHWFKNWKE 
Sbjct: 3   NCKNIFARTQTIRNWSRCNF--NSASSSSSSSSFKFETGNYYSVLQSSSFQHWFKNWKEF 62

Query: 63  KRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNLATGWSNSKEVEALERYKLITG 122
           ++HKLTASTFAGAIGFWPRRRA+LWLEKLGA +QF GNLAT WSN KE EALERYKLITG
Sbjct: 63  RKHKLTASTFAGAIGFWPRRRAQLWLEKLGAIDQFSGNLATCWSNMKEEEALERYKLITG 122

Query: 123 NTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLPSRGVLEIKCPFFDGDMRKASPWS 182
           N+VLFPEFQVY K NSE DWLAASPDGAIDK+VYGLPSRGVLEIKCPFFDGDM KASPWS
Sbjct: 123 NSVLFPEFQVYEKGNSEYDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFDGDMAKASPWS 182

Query: 183 RVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDVEYWKVLKMALSDFWWKHVQ 242
           RVPLY IPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRD EYW+VLK+ALSDFWWKHVQ
Sbjct: 183 RVPLYCIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLYRDAEYWEVLKIALSDFWWKHVQ 242

Query: 243 PARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESKRIVDNSKLLLREFDGKLQT 299
           PARE+CSKY+ITNPLIELKSLRPSP+HELCSYIVCESKR+VDNS+LLLREF+G+LQT
Sbjct: 243 PAREMCSKYSITNPLIELKSLRPSPKHELCSYIVCESKRVVDNSELLLREFNGRLQT 297

BLAST of MS001068 vs. TAIR 10
Match: AT1G13810.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.6e-73
Identity = 128/257 (49.81%), Postives = 169/257 (65.76%), Query Frame = 0

Query: 41  KYHSVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWPRRRAELWLEKLGATEQFCGNL 100
           K  +V+ +    HW KNW++L++++LTAS FA AIGF P  R  LWLEK+GA + F GN 
Sbjct: 45  KEEAVVVTEPTHHWRKNWEDLRKNRLTASNFARAIGFSPDGRRNLWLEKIGAAKPFAGNR 104

Query: 101 ATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANS-EDDWLAASPDGAIDKIVYGLPS 160
           AT W    EVEALERY  +TGN +L PEF VY    S E++WL ASPDG I+ +  G+ S
Sbjct: 105 ATFWDIENEVEALERYNELTGNEILIPEFVVYKNGESPEENWLGASPDGVINVVKDGVTS 164

Query: 161 RGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRLY 220
            GVLE+KCPF + D  K  PW +VP   +PQ QGLMEI+D DW+D Y WT  GSSLFR++
Sbjct: 165 CGVLEVKCPFDNRDNSKVYPWKKVPYNCVPQLQGLMEIVDTDWLDLYCWTRNGSSLFRVW 224

Query: 221 RDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCESK 280
           RD  +W+ +K AL DFW  HV PARE+ + + I +P ++L+  +P   HE C  I+  ++
Sbjct: 225 RDTAFWEDMKPALFDFWQNHVLPAREIYNNFDIKDPQVKLREFKPKHWHEDCKKIMRGAE 284

Query: 281 RIVDNSKLLLREFDGKL 297
           RI  N+  L  E DG L
Sbjct: 285 RISANANRLFYEIDGNL 301

BLAST of MS001068 vs. TAIR 10
Match: AT1G67660.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.3e-47
Identity = 98/253 (38.74%), Postives = 137/253 (54.15%), Query Frame = 0

Query: 44  SVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWP-RRRAELWLEKLGATE----QFCG 103
           S+L  S      + W  L++ KLT STF+ A+GFW   RRAELW EK+  ++    +   
Sbjct: 107 SLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRAELWHEKVYDSDARVVEESA 166

Query: 104 NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 163
             A  W    E  A+ERYK I G  V    F ++  +N E  WL ASPDG +D       
Sbjct: 167 RFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIH--SNEEFHWLGASPDGILDCF----- 226

Query: 164 SRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 223
             G+LE+KCP+  G      PW +VP Y +PQ QG MEIMDR+W++ Y WT  GS++FR+
Sbjct: 227 --GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRV 286

Query: 224 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 283
            RD  YW+++   L +FWW+ V PARE      +     E+K   P+  H+     + +S
Sbjct: 287 MRDRSYWRIIHDVLREFWWESVIPARE---ALLLGKEDEEVKKYEPTSTHKRTKLAIAKS 346

Query: 284 KRIVDNSKLLLRE 292
             +   SKL+ RE
Sbjct: 347 LNLAAESKLVCRE 347

BLAST of MS001068 vs. TAIR 10
Match: AT1G67660.2 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.3e-47
Identity = 98/253 (38.74%), Postives = 137/253 (54.15%), Query Frame = 0

Query: 44  SVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWP-RRRAELWLEKLGATE----QFCG 103
           S+L  S      + W  L++ KLT STF+ A+GFW   RRAELW EK+  ++    +   
Sbjct: 57  SLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRAELWHEKVYDSDARVVEESA 116

Query: 104 NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 163
             A  W    E  A+ERYK I G  V    F ++  +N E  WL ASPDG +D       
Sbjct: 117 RFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIH--SNEEFHWLGASPDGILDCF----- 176

Query: 164 SRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 223
             G+LE+KCP+  G      PW +VP Y +PQ QG MEIMDR+W++ Y WT  GS++FR+
Sbjct: 177 --GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRV 236

Query: 224 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 283
            RD  YW+++   L +FWW+ V PARE      +     E+K   P+  H+     + +S
Sbjct: 237 MRDRSYWRIIHDVLREFWWESVIPARE---ALLLGKEDEEVKKYEPTSTHKRTKLAIAKS 296

Query: 284 KRIVDNSKLLLRE 292
             +   SKL+ RE
Sbjct: 297 LNLAAESKLVCRE 297

BLAST of MS001068 vs. TAIR 10
Match: AT1G67660.3 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.3e-47
Identity = 98/253 (38.74%), Postives = 137/253 (54.15%), Query Frame = 0

Query: 44  SVLQSSSFQHWFKNWKELKRHKLTASTFAGAIGFWP-RRRAELWLEKLGATE----QFCG 103
           S+L  S      + W  L++ KLT STF+ A+GFW   RRAELW EK+  ++    +   
Sbjct: 86  SLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRAELWHEKVYDSDARVVEESA 145

Query: 104 NLATGWSNSKEVEALERYKLITGNTVLFPEFQVYGKANSEDDWLAASPDGAIDKIVYGLP 163
             A  W    E  A+ERYK I G  V    F ++  +N E  WL ASPDG +D       
Sbjct: 146 RFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIH--SNEEFHWLGASPDGILDCF----- 205

Query: 164 SRGVLEIKCPFFDGDMRKASPWSRVPLYSIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRL 223
             G+LE+KCP+  G      PW +VP Y +PQ QG MEIMDR+W++ Y WT  GS++FR+
Sbjct: 206 --GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRV 265

Query: 224 YRDVEYWKVLKMALSDFWWKHVQPARELCSKYAITNPLIELKSLRPSPRHELCSYIVCES 283
            RD  YW+++   L +FWW+ V PARE      +     E+K   P+  H+     + +S
Sbjct: 266 MRDRSYWRIIHDVLREFWWESVIPARE---ALLLGKEDEEVKKYEPTSTHKRTKLAIAKS 325

Query: 284 KRIVDNSKLLLRE 292
             +   SKL+ RE
Sbjct: 326 LNLAAESKLVCRE 326