MS001059 (gene) Bitter gourd (TR) v1

BLAST of MS001059 vs. NCBI nr
Match: XP_022131437.1 (phosphopantothenate--cysteine ligase 2-like [Momordica charantia])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 322/324 (99.38%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MDENGPQTPEETLDAEIKSFFDSAPPLRNIENIS SLKNFIEINSSPAG+RSARKVVCVT
Sbjct: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISESLKNFIEINSSPAGSRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL
Sbjct: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFDH 325
           DEVENHIVKHIVEKHSTYINVFDH
Sbjct: 301 DEVENHIVKHIVEKHSTYINVFDH 324

BLAST of MS001059 vs. NCBI nr
Match: XP_023536743.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556.6 bits (1433), Expect = 1.4e-154
Identity = 281/323 (87.00%), Postives = 297/323 (91.95%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD +G Q  +E LD EIKSFFDSAPPLRNIEN+S  LK FIE+NSS  G RSARKVVCVT
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSS--GTRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  TCQPYCSLLPD PF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFT +SGIQVRQ YSEAVK AISEHHAAVADGTLLKLPFTTIFEYLQ+LH++ TSL
Sbjct: 121 LECFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGP+ALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKA+AAL+KYKMHMVIANEL TRKEEVTLVT+NE IHVRRDPK VG
Sbjct: 241 CISFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFD 324
           DEVE HI++HIVEKHSTY++  D
Sbjct: 301 DEVEKHIIEHIVEKHSTYVDNLD 321

BLAST of MS001059 vs. NCBI nr
Match: XP_008445369.1 (PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >XP_008445370.1 PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >XP_016900047.1 PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >KAA0064808.1 phosphopantothenate--cysteine ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 554.7 bits (1428), Expect = 5.4e-154
Identity = 278/324 (85.80%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD NG Q  +E LDAEIKSFF+SAPPLRNIE+I   L+ F+E NS  AGN+SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  T QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LEC EFT +SGIQ RQ YSEAVK+AISEHHAA+A+GTLLKLPFTTIFEYLQMLHL+  S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKADAALRKYKMHMVIANEL TRKEEVTLVT NEKIHVRRDPKLVG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFDH 325
           D VE HIVKHIV+KHS Y++ FDH
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDH 324

BLAST of MS001059 vs. NCBI nr
Match: XP_023002782.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita maxima])

HSP 1 Score: 553.9 bits (1426), Expect = 9.1e-154
Identity = 280/323 (86.69%), Postives = 296/323 (91.64%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD +G Q  +E LD EIKSFFDSAPPLRNIEN+S  LK FIE+NSS  G RSARKVVCVT
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSS--GTRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGY VIFLYR  TCQPYCSLLPD PF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYLVIFLYRKGTCQPYCSLLPDYPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFT +SGIQVRQ YSEAVK AISEHHAAVADGTLLKLPFTTIFEYLQ+LH++ TSL
Sbjct: 121 LECFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGP+ALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPNALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKA+AAL+KYKMHMVIANEL TRKEEVTLVT+NE IHVRRDPK VG
Sbjct: 241 CISFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFD 324
           DEVE HI++HIVEKHSTY++  D
Sbjct: 301 DEVEKHIIEHIVEKHSTYVDNLD 321

BLAST of MS001059 vs. NCBI nr
Match: XP_022952050.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita moschata])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 279/323 (86.38%), Postives = 296/323 (91.64%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD +G Q  +E LD EIKSFFDSAPPLRNIEN+S  LK FIE+NSS  G RSARKVVCVT
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSS--GTRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQ+CVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  TCQPYCSLLPD PF
Sbjct: 61  SGGTTVPLEQKCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFT  SGIQVRQ YSEAVK AISEHHAAVADGTLLKLPFTTIFEYLQ+LH++ TSL
Sbjct: 121 LECFEFTHGSGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGP+ALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKA+AAL+KYKMHMVIANEL TRKEEVTLVT+NE IHVRRDPK VG
Sbjct: 241 CISFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFD 324
           +EVE HI++HIVEKHSTY++  D
Sbjct: 301 NEVEKHIIEHIVEKHSTYVDDLD 321

BLAST of MS001059 vs. ExPASy Swiss-Prot
Match: Q9LZM3 (Phosphopantothenate--cysteine ligase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCS2 PE=2 SV=2)

HSP 1 Score: 411.8 bits (1057), Expect = 7.4e-114
Identity = 205/307 (66.78%), Postives = 252/307 (82.08%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRC 72
           ++ EI SFF+S+PP +N+E I  +L  FI++NSS  G    R++VCVTSGGTTVPLEQRC
Sbjct: 1   MEDEISSFFESSPPQKNMEEILENLNEFIKLNSSSQG---GRRIVCVTSGGTTVPLEQRC 60

Query: 73  VRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGI 132
           VRYIDNFSSG+RGAASTE F+KAGY+VIFLYR  TCQPYC  LPDDPFLECFEF+  + I
Sbjct: 61  VRYIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRSLPDDPFLECFEFSDKTNI 120

Query: 133 QVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLA 192
           QV  S+ EAVK A+ +   AVA+G+LLKLPF+TI+EYLQML LI  +L+ +GP ++FYLA
Sbjct: 121 QVHTSHLEAVKMAVMDQQTAVAEGSLLKLPFSTIYEYLQMLRLIAEALKDVGPCSMFYLA 180

Query: 193 AAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKI 252
           AAVSDFYVPW+SM EHKI+SGSGPLD+RLAQVPKMLSVLRS WAP A+CISFKLETD KI
Sbjct: 181 AAVSDFYVPWKSMTEHKIESGSGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKI 240

Query: 253 LLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIV 312
           L+EKA  ALRKYK+H V+ANELSTRKEEV +V+ +  + VRR+       VE+++++ IV
Sbjct: 241 LMEKATKALRKYKVHAVVANELSTRKEEVVVVSSSGNVVVRRECDKPESFVEDNLIRLIV 300

Query: 313 EKHSTYI 320
           ++HSTYI
Sbjct: 301 DRHSTYI 304

BLAST of MS001059 vs. ExPASy Swiss-Prot
Match: Q8GXR5 (Phosphopantothenate--cysteine ligase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCS1 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.6e-113
Identity = 207/307 (67.43%), Postives = 248/307 (80.78%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRC 72
           ++ EI SFF+S+PPL+N E I  +L  FIE+NSS  G    R++VCVTSGGTTVPLEQRC
Sbjct: 8   VEDEISSFFESSPPLKNKEEIVANLNQFIELNSSCQG--GMRRIVCVTSGGTTVPLEQRC 67

Query: 73  VRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGI 132
           VRYIDNFSSG+RGAASTE F+KAGY+VIFLYR  TCQPYC  LPDDPFLECFEF     I
Sbjct: 68  VRYIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRYLPDDPFLECFEFPDAKNI 127

Query: 133 QVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLA 192
           QV  S+S AVK A+ +  AAVA+G LLKLPF+TI+EYLQML LI T L+ +GP ++FYLA
Sbjct: 128 QVHGSHSGAVKMAVMDQQAAVAEGRLLKLPFSTIYEYLQMLRLIATILKDVGPCSMFYLA 187

Query: 193 AAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKI 252
           AAVSDFYVPW SM EHKI+SGSGPLD+RLAQVPKMLS+LRS WAP A+CISFKLETD KI
Sbjct: 188 AAVSDFYVPWLSMTEHKIESGSGPLDIRLAQVPKMLSILRSNWAPKAFCISFKLETDSKI 247

Query: 253 LLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIV 312
           LLEKA  AL+KYK+H V+ANEL TRKEEV +V+ +  + VRRD       VE+++++ +V
Sbjct: 248 LLEKATKALQKYKVHAVVANELLTRKEEVVVVSSSGNVVVRRDSNKPESIVEDNLIRLLV 307

Query: 313 EKHSTYI 320
           ++HSTYI
Sbjct: 308 DRHSTYI 312

BLAST of MS001059 vs. ExPASy Swiss-Prot
Match: Q69S81 (Phosphopantothenate--cysteine ligase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0575200 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-103
Identity = 189/303 (62.38%), Postives = 238/303 (78.55%), Query Frame = 0

Query: 18  KSFFDSAPPLRNIENISGSLKNFIEINSSPAG-NRSARKVVCVTSGGTTVPLEQRCVRYI 77
           +SFF +APPLR+ + ++  L  FI  NSS AG     R++VCVTSGGTTVPLEQRCVRYI
Sbjct: 9   ESFFAAAPPLRDADAVAARLGEFIARNSSAAGAGGGGRRIVCVTSGGTTVPLEQRCVRYI 68

Query: 78  DNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGIQVRQ 137
           DNFSSGHRGAASTEYFLKAGY+VIFL+R  +CQPYC  LPDD FL+ F+   +S +QV +
Sbjct: 69  DNFSSGHRGAASTEYFLKAGYAVIFLHRRGSCQPYCRFLPDDSFLKFFDVDAESKVQVAE 128

Query: 138 SYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLAAAVS 197
            ++  VK AI ++  A+  G LLKLPFTTIFEYLQ+L ++ TS+ S GP  +FYLAAAVS
Sbjct: 129 CHAPVVKKAIGDYCKAIEGGYLLKLPFTTIFEYLQLLKMVATSISSAGPLGMFYLAAAVS 188

Query: 198 DFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKILLEK 257
           DFYVPW+SMA+HKIQSG GPLDMRL+QVPKMLSVLR++WAP A+CISFKLETD  IL++K
Sbjct: 189 DFYVPWDSMAKHKIQSGGGPLDMRLSQVPKMLSVLRNQWAPLAFCISFKLETDSDILIQK 248

Query: 258 ADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIVEKHS 317
           AD AL KYKM++V+AN L+T KEEV +VT+ E+  +R+  K   +++E  I+K + + HS
Sbjct: 249 ADMALNKYKMNIVVANLLATYKEEVIIVTDKERSTIRKMNK--DEDLEMQIIKILSQNHS 308

Query: 318 TYI 320
            YI
Sbjct: 309 KYI 309

BLAST of MS001059 vs. ExPASy Swiss-Prot
Match: Q0J7N5 (Phosphopantothenate--cysteine ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0176100 PE=2 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 4.6e-100
Identity = 183/304 (60.20%), Postives = 234/304 (76.97%), Query Frame = 0

Query: 16  EIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRCVRY 75
           E +SFF +APPLR+ + ++G L +F+  +S   G      VVCVTSGGTTVPLEQRCVRY
Sbjct: 11  EAESFFRAAPPLRDQDRVAGDLADFVARHSGSGGGGRLAGVVCVTSGGTTVPLEQRCVRY 70

Query: 76  IDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGIQVR 135
           IDNFSSG RGAASTEYFLKAGY+VIF+YR  + QPYC  LP+D FL+ FE   +S IQV 
Sbjct: 71  IDNFSSGQRGAASTEYFLKAGYAVIFIYRRGSKQPYCRFLPEDSFLDLFELGEESDIQVP 130

Query: 136 QSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLAAAV 195
           +S++  VK+AI  +  A+ +G LLKLPFTTIFEYLQ+L ++GT++  LG   +FYLAAAV
Sbjct: 131 ESHAAVVKTAIRNYRKAIDEGLLLKLPFTTIFEYLQLLQMVGTAMNCLGRQGMFYLAAAV 190

Query: 196 SDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKILLE 255
           SDFYVPWESMA+HKI+S SGPL+M+L QVPKML +LR +WAP+A+C+SFKLETD  ILL+
Sbjct: 191 SDFYVPWESMAKHKIESASGPLNMQLNQVPKMLFILRKQWAPSAFCVSFKLETDPDILLQ 250

Query: 256 KADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIVEKH 315
           KA+AALRKY M++V+ANEL+  K+ V +VT N +  VRR  K   D+VE  ++  +VE H
Sbjct: 251 KAEAALRKYGMNVVVANELANYKDVVVMVTSNGRTTVRRPSK--EDDVEEQLIDLLVEMH 310

Query: 316 STYI 320
           S +I
Sbjct: 311 SEHI 312

BLAST of MS001059 vs. ExPASy Swiss-Prot
Match: Q9USK7 (Uncharacterized protein C4B3.18 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC4B3.18 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-57
Identity = 133/310 (42.90%), Postives = 187/310 (60.32%), Query Frame = 0

Query: 20  FFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRCVRYIDNF 79
           FF+  P   ++ ++S  +++FI + S      +++ +  VTSGGT VPLEQ  VR+IDNF
Sbjct: 10  FFEENPCPNSLGDVSSLIEDFINLQS------NSQNIAFVTSGGTLVPLEQNTVRFIDNF 69

Query: 80  SSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGIQ---VRQ 139
           S+G+RGAAS EYF K+GYSVIFLYR  +  P+     + P+   FE +  S  +   V+Q
Sbjct: 70  SAGNRGAASAEYFCKSGYSVIFLYRNYSLMPFTRRY-EGPWTNLFERSNGSQEKPWIVKQ 129

Query: 140 SYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLAAAVS 199
            +   V +A++E         L+ +P+TT+ EYL  L  I   L+ +   ALFYLAAAVS
Sbjct: 130 EFQSTVFNALNELDTYTKHHRLIMIPYTTLTEYLWYLKAIAERLQIIASRALFYLAAAVS 189

Query: 200 DFYVPWESMAEHKIQSGSG--PLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKILL 259
           DF++P + ++EHKIQSGSG   L++++  VPK L  L   WAP A  ISFKLETD  IL+
Sbjct: 190 DFHIPPKELSEHKIQSGSGSNKLEVKMHPVPKFLRFLVDTWAPEASIISFKLETDPSILI 249

Query: 260 EKADAALRKYKMHMVIANELSTRKEEVTLVTEN--EKIHVRRDPKLVGDEVENHIVKHIV 319
           +KA  AL++Y   +VIAN LSTRK  V  VT    E +H+  D  +   E+E +IV   +
Sbjct: 250 DKARVALQRYNHQLVIANLLSTRKRSVAFVTSKTVEWLHLSDDDIVKKVEIEQYIVSRAI 309

Query: 320 EKHSTYINVF 323
             H   I  F
Sbjct: 310 SLHKERIQEF 312

BLAST of MS001059 vs. ExPASy TrEMBL
Match: A0A6J1BR05 (phosphopantothenate--cysteine ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111004651 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.5e-181
Identity = 322/324 (99.38%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MDENGPQTPEETLDAEIKSFFDSAPPLRNIENIS SLKNFIEINSSPAG+RSARKVVCVT
Sbjct: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISESLKNFIEINSSPAGSRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL
Sbjct: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFDH 325
           DEVENHIVKHIVEKHSTYINVFDH
Sbjct: 301 DEVENHIVKHIVEKHSTYINVFDH 324

BLAST of MS001059 vs. ExPASy TrEMBL
Match: A0A1S3BCJ3 (phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103488424 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.6e-154
Identity = 278/324 (85.80%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD NG Q  +E LDAEIKSFF+SAPPLRNIE+I   L+ F+E NS  AGN+SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  T QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LEC EFT +SGIQ RQ YSEAVK+AISEHHAA+A+GTLLKLPFTTIFEYLQMLHL+  S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKADAALRKYKMHMVIANEL TRKEEVTLVT NEKIHVRRDPKLVG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFDH 325
           D VE HIVKHIV+KHS Y++ FDH
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDH 324

BLAST of MS001059 vs. ExPASy TrEMBL
Match: A0A5A7VB27 (Phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001160 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.6e-154
Identity = 278/324 (85.80%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD NG Q  +E LDAEIKSFF+SAPPLRNIE+I   L+ F+E NS  AGN+SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  T QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LEC EFT +SGIQ RQ YSEAVK+AISEHHAA+A+GTLLKLPFTTIFEYLQMLHL+  S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKADAALRKYKMHMVIANEL TRKEEVTLVT NEKIHVRRDPKLVG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFDH 325
           D VE HIVKHIV+KHS Y++ FDH
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDH 324

BLAST of MS001059 vs. ExPASy TrEMBL
Match: A0A6J1KKG1 (phosphopantothenate--cysteine ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111496541 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.4e-154
Identity = 280/323 (86.69%), Postives = 296/323 (91.64%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD +G Q  +E LD EIKSFFDSAPPLRNIEN+S  LK FIE+NSS  G RSARKVVCVT
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSS--GTRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGY VIFLYR  TCQPYCSLLPD PF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYLVIFLYRKGTCQPYCSLLPDYPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFT +SGIQVRQ YSEAVK AISEHHAAVADGTLLKLPFTTIFEYLQ+LH++ TSL
Sbjct: 121 LECFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGP+ALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPNALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKA+AAL+KYKMHMVIANEL TRKEEVTLVT+NE IHVRRDPK VG
Sbjct: 241 CISFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFD 324
           DEVE HI++HIVEKHSTY++  D
Sbjct: 301 DEVEKHIIEHIVEKHSTYVDNLD 321

BLAST of MS001059 vs. ExPASy TrEMBL
Match: A0A6J1GKN1 (phosphopantothenate--cysteine ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111454754 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 279/323 (86.38%), Postives = 296/323 (91.64%), Query Frame = 0

Query: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVT 60
           MD +G Q  +E LD EIKSFFDSAPPLRNIEN+S  LK FIE+NSS  G RSARKVVCVT
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSS--GTRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120
           SGGTTVPLEQ+CVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR  TCQPYCSLLPD PF
Sbjct: 61  SGGTTVPLEQKCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPF 120

Query: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180
           LECFEFT  SGIQVRQ YSEAVK AISEHHAAVADGTLLKLPFTTIFEYLQ+LH++ TSL
Sbjct: 121 LECFEFTHGSGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240
           RSLGP+ALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300
           CISFKLETDVKILLEKA+AAL+KYKMHMVIANEL TRKEEVTLVT+NE IHVRRDPK VG
Sbjct: 241 CISFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVG 300

Query: 301 DEVENHIVKHIVEKHSTYINVFD 324
           +EVE HI++HIVEKHSTY++  D
Sbjct: 301 NEVEKHIIEHIVEKHSTYVDDLD 321

BLAST of MS001059 vs. TAIR 10
Match: AT1G12350.1 (4-phospho-panto-thenoylcysteine synthetase )

HSP 1 Score: 410.6 bits (1054), Expect = 1.2e-114
Identity = 207/307 (67.43%), Postives = 248/307 (80.78%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLRNIENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRC 72
           ++ EI SFF+S+PPL+N E I  +L  FIE+NSS  G    R++VCVTSGGTTVPLEQRC
Sbjct: 8   VEDEISSFFESSPPLKNKEEIVANLNQFIELNSSCQG--GMRRIVCVTSGGTTVPLEQRC 67

Query: 73  VRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGI 132
           VRYIDNFSSG+RGAASTE F+KAGY+VIFLYR  TCQPYC  LPDDPFLECFEF     I
Sbjct: 68  VRYIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRYLPDDPFLECFEFPDAKNI 127

Query: 133 QVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLA 192
           QV  S+S AVK A+ +  AAVA+G LLKLPF+TI+EYLQML LI T L+ +GP ++FYLA
Sbjct: 128 QVHGSHSGAVKMAVMDQQAAVAEGRLLKLPFSTIYEYLQMLRLIATILKDVGPCSMFYLA 187

Query: 193 AAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKI 252
           AAVSDFYVPW SM EHKI+SGSGPLD+RLAQVPKMLS+LRS WAP A+CISFKLETD KI
Sbjct: 188 AAVSDFYVPWLSMTEHKIESGSGPLDIRLAQVPKMLSILRSNWAPKAFCISFKLETDSKI 247

Query: 253 LLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIV 312
           LLEKA  AL+KYK+H V+ANEL TRKEEV +V+ +  + VRRD       VE+++++ +V
Sbjct: 248 LLEKATKALQKYKVHAVVANELLTRKEEVVVVSSSGNVVVRRDSNKPESIVEDNLIRLLV 307

Query: 313 EKHSTYI 320
           ++HSTYI
Sbjct: 308 DRHSTYI 312

BLAST of MS001059 vs. TAIR 10
Match: AT5G02080.1 (DNA / pantothenate metabolism flavoprotein )

HSP 1 Score: 360.9 bits (925), Expect = 1.1e-99
Identity = 186/290 (64.14%), Postives = 224/290 (77.24%), Query Frame = 0

Query: 30  IENISGSLKNFIEINSSPAGNRSARKVVCVTSGGTTVPLEQRCVRYIDNFSSGHRGAAST 89
           +E I  +L  FI++NSS  G    R++VCVTSGGTTVPLEQRCVRYIDNFSSG+RGAAST
Sbjct: 1   MEEILENLNEFIKLNSSSQG---GRRIVCVTSGGTTVPLEQRCVRYIDNFSSGNRGAAST 60

Query: 90  EYFLKAGYSVIFLYRTKTCQPYCSLLPDDPFLECFEFTCDSGIQVRQSYSEAVKSAISEH 149
           E F+KAGY+VIFLYR  TCQPYC  LPDDPFLECFEF+  + IQ                
Sbjct: 61  ENFVKAGYAVIFLYRRGTCQPYCRSLPDDPFLECFEFSDKTNIQ---------------- 120

Query: 150 HAAVADGTLLKLPFTTIFEYLQMLHLIGTSLRSLGPHALFYLAAAVSDFYVPWESMAEHK 209
                 G+LLKLPF+TI+EYLQML LI  +L+ +GP ++FYLAAAVSDFYVPW+SM EHK
Sbjct: 121 ------GSLLKLPFSTIYEYLQMLRLIAEALKDVGPCSMFYLAAAVSDFYVPWKSMTEHK 180

Query: 210 IQSGSGPLDMRLAQVPKMLSVLRSEWAPTAYCISFKLETDVKILLEKADAALRKYKMHMV 269
           I+SGSGPLD+RLAQVPKMLSVLRS WAP A+CISFKLETD KIL+EKA  ALRKYK+H V
Sbjct: 181 IESGSGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKILMEKATKALRKYKVHAV 240

Query: 270 IANELSTRKEEVTLVTENEKIHVRRDPKLVGDEVENHIVKHIVEKHSTYI 320
           +ANELSTRKEEV +V+ +  + VRR+       VE+++++ IV++HSTYI
Sbjct: 241 VANELSTRKEEVVVVSSSGNVVVRRECDKPESFVEDNLIRLIVDRHSTYI 265