MELO3C025585 (gene) Melon (DHL92) v4

Overview
NameMELO3C025585
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Descriptionbifunctional nitrilase/nitrile hydratase NIT4A
Locationchr12: 12666728 .. 12671593 (-)
RNA-Seq ExpressionMELO3C025585
SyntenyMELO3C025585
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACTCGAGTCCATACATAGCTTAGCCATTGATAGCACTCTTTCTCGCCGGCTGAAGCTCAAGCTGAGATAGAGAAAAAAAAAAGGGAGATTCAAGCAGCAACCATGGCGCTTGTACCAACCACGGCCCCTACCGATCCCCAATTGATAGCGGAGGTTGACATGGGCACCGATTCATCTGTAATGACTGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGGTTCCTTTTCCTTGCTTCCACTTTCAATTTACTCCTGATGTACTTCTCTTTCTCCTGGTGTTGCTTCGTTCACTAGCTCTTGGATCGCAATGGTCTGTCCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTTTTCTTCGAAGCAAGCTTATTACTTGTTTTGGCTCTTTTTTTGCTTGGCTTTCTTTAATCTTACAAGTTGCTTTCCTTTCATCAGCTGATTTTGGTTGAATAATTTAATTAGTTTAAACTATTACATCTTGGATTATTTTTCCTGGTTTTAATTGTTTCTCTTGTCTGCGATCAATATGAATTTTCTGCTGTCGCCTAACATTGTGCTTCTGATTGTTCTTTTCTCCTTTAATTTTACTCGATTGGTGAAATTGAAGAGAAAATGTAGACGTGAAATACCATGGCTTAAAATGATTTAATTCGTAAGTTCCAAAGGCATTAACAGAGTTGGGGATTATCACGCTTAGATGGAAAATCTTTTAGTATATTTAAGGCAGCTACTAGCCTATGACCATGTGGGCCTTTTTTTTCCTTCATCTGTAATGGTAAAATGAAATCTAACTCTGTTTTACATGAATAGACAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAACTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTTAGTCTTTGTTTCCAGGTGTACTTTGTTTGACTCAATTTGAGTTGCTACTGTAAAGAATAAAATCTTAGCGTCTTACCATGGATAATTTCAGGTTAAGTAATTTTTGCTAGGCTCATTGTATCATTCTCATTCTAGAGGGCTGACTTAATTTCTCCATGAGTTTAAACTTGATTTCTGGTGATATATTAATATTAAATTCTTTCATCGTAACATACACCTTCTGTTCTTTATCATTTTTCATTCTTCAAATGGATGTTACCTCATATTAGGACAGTACAATAAGGAGCTACGGATGTCGATCTATTCCAAGTGAAATACGAGTGTGTTGTTCATAAAATGTGAAATGGGACTATGGTCTGTTTTAATGGTTCTTGTAATTTGCAGTCAATGAATGACAAACAAGATTGCTTCAACTACTGGCATTTCTGGGTTCTATTAATTTCAAAATCTTCCTAATCTTGCTTGCATCTAGAACCTAGACATAATATTGTAAAGTTCTGAAGGCTGTAGCCAGTTCCTGTCATGGTTGCGGTTTAGCTTTGTTTTGAAACTCTATTTGCTAGGCTTCAGTTCATGTACGAGGTTGCTTTACTTCCATTCATTCTAATGCAATCTGGTTGGCCTTGTATTTACTTCTATTTGTCTACGTAATATCTTGTTTTCACATAACTAATTATCTAAAACCATTTTTATTCATCTATAAGATCATATGCCACAATCACAAAATAAACTGCTCGAAGTGCACCGGCTGTGGTTCTTTAAATGATTAATGTTTTGATGACTATAGACGGTAATACATGCAGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTTCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTATACACTGTACTGTACTGTCCTATTTTTCGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCGACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCTATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGTTAGTTATTCATTGCTGGCAACAATGACATTCCTGAAGCAATTGCATATTTAAAGTCCAGTTTTATTCTTTTTGTTTGACTGTTAGGTGTTTATTAACTTAAAATAGATAGCACGATAAAATTGTGATAGGAATGGTATTAGAGGGATATTAAGGGTATATTAGTAGTTAGTTAACAAGTTTGTCAGGGAAGTTTGGTTCTAAATGTAATGAGTAGGGAAGAAAGGGGTAGACAATATTTTGGGAGTAAATTAGGGCTTTGAGTGATCTCAAGATTGAGAGGGTCTAAGTACCTCAAATTACATGGTCTATTTTGTATTTCTGTTCTCTTTTATTTGTTCTACCAAAATACTGACGAGAAACTATTAGAGACTAAGAGGCTCTAGTTGTTAAGAGATGGTAAAGTGATGGTCAAACTCCAGAGTTGGGGATTATTGTGTCGCTGCTCACTATTTTACAGACACTGGTCGATCATTTTCAGCTTCTTTTCTCAGAATATACTTGTTTGGTTAATCATATTATGCACAACCTTTTTGTTTTATGTTTCTCTTTCAAGTACATCAAGTTAAAGTGTACTCTAAACCAGATGGAAATGATCATATAGAAGAAGGTTTTCATATGCTTAAAGTTCTACTGCTATGTCAAGGATAGAAGTTGCTATTTCTTCAAAGTTTCCTAACTTCCGTCTACACATTCCTTTTCTTTTACTTAATAGAAGTTGCTCACTAGAAATTGACTTATCATGTTTGTAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACGGAAGAGGAACTGACCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGCACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGGTATGTCATCTCATCCTTCTGCTTATGGTGATCAGAGCTTTAGTTCTGTACTCCAACATTATCATGTTAAGTGGTTGATATAAGAAGGTTTTATCTTGAGGAACCTACCGTTTGATTTTAGTCTCTTTCTTCTTCAAGCGAGTCCTAGGATTCTCTCTCCTGGATGATCTGATGCTCCTGACAGCCAACGAGTCTGAAAACCTTTATTCCAAGCTCTTATTCTATGCTTGGCCATTTAGTGTCAACATAAATGAACGGCCACTAGGATTCAGTTTCCAATAAAATGGTTAAGGATCAATCAACCATTAAAGAGAGGCAGGGCCTCTCTTGCTGTCTAAGAAGTTCCTTGGAGAATGTGGGGATAGTCCGAAGTGGTTGTTTTGAACCTTACATGCCTTGAGTTGTGAAACTTTTTCATTTAGATCTGTGTGAGCAAAGGTCTAACTAGGAATTCCTACGCTAAGCCTCTTACCTAGGTAGAAGGGTTTTTGTGAAGGCTCTACTTGATAATATAAATAGAAAGCACAAAATTACATGGACAAGGGAATAACAACCATCTAACCACGAAAGGCCGAATAAAGGCCCAATCAAATCCCTTCAGGGAAAATTTAGATTTACAGTGGTTCTCACAGTTCGAATATTGAAGAATGTTAGATACCAAAGAGAGCTCCTAATATTTGACTGGTCAGGAAGACAAGAAGCAATAAAGCAAGAAAGAGTTATTGGCTTATCGCCATCGGATATTTAACCTCCTAAGACAGTCCCTTTTAGAAAAACACACAAAAAAGAACCCCCCAAGATCGGTCACTAATGCCAGCTACTTAATCATAAGAGATTGGTCTTGAAGCCAAACATATTCTTCAATGAATAAAGGATTGAAGATGCATATTCAAATCTCCCAAGCATATGCCTGCAAATAAAGGTTGGAGAGATGCATATTCAAATCTCCCATAATGTGCTATTTCAGAATGGAGTTTATTAAGCATATCTAAAATACTTCTGCAGCCATAACTCATAAAAATGAGAGAACATTTCGACTAGTTAAAAATGCCTTAAGCAAATAAAGAACACTTGAATATTAATTTGTTTCCCTTAACAAGTGGAGCAAAAGGCGTTTATAAGTTTTCACATGCTCTGCTTTTGAACGGTGAAATTCTTGGTTTGAAATTGTGGCAAGCCATTGATTTTTGGCACGGTGCAATATGAATAATTATTGATCCATCCATCCAAACTCTTAACAAATTAAACTTTTTGGGGCATATGTGTTATTGGCTATGTACCCCTCCCTTCATTGATTGTTATGGAAAGCTTTTTCTACCACTAGCATTTTTAGATGAAAAATAATTTGGATGAATATCTGAAACAGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCACTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTGCAATCCCGGTTACTTTCACATCTACATCTACAAAAGCCGAAGATTCTTGTAAAAAGTAGTATGTATTATTCGTCCACACTTCTATTTAGTTCCATTAATTGTTCTAGCCTACATTCGTTCATGTTTCACTGTTTCCTCCGCGTTCAATGTTGTATTATATTCACACTAAGTAATTCAAAACTGTGAATATTTCTGTTAATTTTTATCAGAATTATATAGCAGCAATTTATCACGGTGGGTTCCTTTT

mRNA sequence

AAAACTCGAGTCCATACATAGCTTAGCCATTGATAGCACTCTTTCTCGCCGGCTGAAGCTCAAGCTGAGATAGAGAAAAAAAAAAGGGAGATTCAAGCAGCAACCATGGCGCTTGTACCAACCACGGCCCCTACCGATCCCCAATTGATAGCGGAGGTTGACATGGGCACCGATTCATCTGTAATGACTGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGACAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAACTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTTCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTATACACTGTACTGTACTGTCCTATTTTTCGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCGACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCTATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACGGAAGAGGAACTGACCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGCACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCACTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTGCAATCCCGGTTACTTTCACATCTACATCTACAAAAGCCGAAGATTCTTGTAAAAAGTAGTATGTATTATTCGTCCACACTTCTATTTAGTTCCATTAATTGTTCTAGCCTACATTCGTTCATGTTTCACTGTTTCCTCCGCGTTCAATGTTGTATTATATTCACACTAAGTAATTCAAAACTGTGAATATTTCTGTTAATTTTTATCAGAATTATATAGCAGCAATTTATCACGGTGGGTTCCTTTT

Coding sequence (CDS)

ATGGCGCTTGTACCAACCACGGCCCCTACCGATCCCCAATTGATAGCGGAGGTTGACATGGGCACCGATTCATCTGTAATGACTGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCCGCCACTCTAGACAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAACTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCGCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTTCATTTGGTAATGGGGGTGATAGAGAGAGATGGCTATACACTGTACTGTACTGTCCTATTTTTCGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCGACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCTATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACGGAAGAGGAACTGACCCCAGATTCTGTTGTTTGTGCTGGAGGTAGTGCCATCATATCACCTTCAGGCACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCACTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTGCAATCCCGGTTACTTTCACATCTACATCTACAAAAGCCGAAGATTCTTGTAAAAAGTAG

Protein sequence

MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK
Homology
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BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match: Q42965 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 9.2e-179
Identity = 305/349 (87.39%), Postives = 324/349 (92.84%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT A  +  L AEVDMG +SS  TVRATVVQASTIFYDTPATL KAERLLAEAA +
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEE+LTPDS+VCAGGS IISPSG +LAGPNY GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH   PV+FTSTS+KAE S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]
BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match: Q42966 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 3.5e-178
Identity = 304/349 (87.11%), Postives = 324/349 (92.84%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT    +  + AEVDMG +SS  TVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGT E+LTPDS+VCAGGS IISPSG +LAGPNY+GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH   PV+FTSTS+KAE S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

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BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match: Q5QGZ8 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.1e-176
Identity = 299/349 (85.67%), Postives = 321/349 (91.98%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALV T    D  L AEV+M +D +  TVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH+AAIDVPGPEVDRLAA+AGK
Sbjct: 61  GAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRR+DYPPPPEYVF GTEE LTPDSVVCAGGS IISPSG +LAGP+Y+GEALISADLD
Sbjct: 241 QFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 350
           LGEIARAKFDFDVVGHY+RPEVLSLVV+DHP  PVTFTS STK ED  K
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]
BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match: Q3LRV4 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 7.3e-176
Identity = 303/351 (86.32%), Postives = 323/351 (92.02%), Query Frame = 0 

Query: 1   MALVPTTAPT--DPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MALV TT PT  D  L AEVDM +  +  TVRATVVQASTIFYDTPATLDKAERLL +AA
Sbjct: 1   MALV-TTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMA 120
            +G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH+AAIDVPGPEVDRL+AMA
Sbjct: 61  SYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMA 120

Query: 121 GKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180
           GKY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVF
Sbjct: 121 GKYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180

Query: 181 ETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 240
           +T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLS
Sbjct: 181 QTPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLS 240

Query: 241 ANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 300
           ANQFCRRKDYPPPPEYVFSGTEE+LTPDSVV AGGS IISPSG +LAGPNY+GEALISAD
Sbjct: 241 ANQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISAD 300

Query: 301 LDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 350
           LDLGEIARAKFDFDVVGHY+R EVLSL+V+DHP  PVTFTSTSTK ED  K
Sbjct: 301 LDLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]
BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match: Q6H849 (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX=39947 GN=NIT4 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 4.9e-164
Identity = 282/349 (80.80%), Postives = 309/349 (88.54%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDM--GTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MA+VP+ +   P +IAEV+M  G DS   TVRATVVQAST+FYDTPATLDKAERL+ EAA
Sbjct: 1   MAMVPSGSGGGPPVIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFG----VSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRL 120
           G+GSQLVVFPEAFVGGYPRGS FG    +SIGN   KGKEEFRKYHAAAI+VPGPEV RL
Sbjct: 61  GYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRL 120

Query: 121 AAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGST 180
           AAMAGKY+V LVMGVIER+GYTLYC+VLFFDP GRYLGKHRK+MPTALERIIWGFGDGST
Sbjct: 121 AAMAGKYKVFLVMGVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGST 180

Query: 181 IPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGC 240
           IPV++T +GKIGA ICWEN+MPLLRTA+Y KGIEIYCAPTADSR  WQASMTHIALEGGC
Sbjct: 181 IPVYDTPLGKIGALICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGGC 240

Query: 241 FVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEAL 300
           FVLSANQFCRRKDYPPPPEYVFSG  EE +PD+VVC GGS IISPSG +LAGPNY+GEAL
Sbjct: 241 FVLSANQFCRRKDYPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEAL 300

Query: 301 ISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTK 344
           I+ADLDLGEI RAKFDFDVVGHYARPEVLSLVV D P +PV+FTS + K
Sbjct: 301 ITADLDLGEIVRAKFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEK 349

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BLAST of MELO3C025585 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA0035558.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa] >TYK30976.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa])

HSP 1 Score: 713.0 bits (1839), Expect = 1.3e-201
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. NCBI nr
Match: XP_004139500.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_022889 [Cucumis sativus])

HSP 1 Score: 700.3 bits (1806), Expect = 8.5e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.0 bits (1774), Expect = 4.4e-194
Identity = 337/350 (96.29%), Postives = 341/350 (97.43%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS STK EDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. NCBI nr
Match: KAG6583706.1 (Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019353.1 Bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 685.6 bits (1768), Expect = 2.2e-193
Identity = 335/350 (95.71%), Postives = 341/350 (97.43%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS +TK EDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. NCBI nr
Match: XP_038894429.1 (bifunctional nitrilase/nitrile hydratase NIT4A isoform X1 [Benincasa hispida])

HSP 1 Score: 685.3 bits (1767), Expect = 2.8e-193
Identity = 336/351 (95.73%), Postives = 344/351 (98.01%), Query Frame = 0 

Query: 1   MALVPTTAPTD-PQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60
           MALVPTTA  D  QLIAEVDMG+DSSV+TVRATVVQASTIFYDTPATL+KAERLLAEAAG
Sbjct: 1   MALVPTTASNDASQLIAEVDMGSDSSVLTVRATVVQASTIFYDTPATLEKAERLLAEAAG 60

Query: 61  FGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           FGSQLVVFPEAFVGGYPRGSNFGV IGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG
Sbjct: 61  FGSQLVVFPEAFVGGYPRGSNFGVCIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180
           KYRVHLVMGVIERDGYTLYCT+LFFDPQGRYLGKHRK+MPTALER+IWGFGDGSTIPVFE
Sbjct: 121 KYRVHLVMGVIERDGYTLYCTILFFDPQGRYLGKHRKLMPTALERLIWGFGDGSTIPVFE 180

Query: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           TSIGKIGAAICWENRMPLLRTA+YAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA
Sbjct: 181 TSIGKIGAAICWENRMPLLRTALYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPPEYVFSGTEEEL+PDSVVCAGGSAIISPSGTILAGPNYDGEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSAIISPSGTILAGPNYDGEALISADL 300

Query: 301 DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           DLGEI RAKFDFDVVGHYARPEVLSLVVRDHPA PVTFTSTSTKAEDSCKK
Sbjct: 301 DLGEIIRAKFDFDVVGHYARPEVLSLVVRDHPATPVTFTSTSTKAEDSCKK 351

[Close]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match: A0A5A7SY83 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002030 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.2e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match: A0A1S3CLB4 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.2e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match: A0A0A0LVI0 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 4.1e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match: A0A6J1I7W0 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LOC111471660 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 334/350 (95.43%), Postives = 338/350 (96.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS S K EDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match: A0A6J1EHI6 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=LOC111434356 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.6e-192
Identity = 333/349 (95.42%), Postives = 338/349 (96.85%), Query Frame = 0 

Query: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS + K EDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]
BLAST of MELO3C025585 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 554.3 bits (1427), Expect = 7.0e-158
Identity = 264/335 (78.81%), Postives = 298/335 (88.96%), Query Frame = 0 

Query: 13  QLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFV 72
           Q+  E+DM    S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQLVVFPEAF+
Sbjct: 20  QIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFI 79

Query: 73  GGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIER 132
           GGYPRGS F ++IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA KY+V+LVMGVIER
Sbjct: 80  GGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKVYLVMGVIER 139

Query: 133 DGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWE 192
           +GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IGKIGAAICWE
Sbjct: 140 EGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIGKIGAAICWE 199

Query: 193 NRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPP 252
           NRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFCRRKDYP PP
Sbjct: 200 NRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFCRRKDYPSPP 259

Query: 253 EYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFD 312
           EY+FSG+EE LTPDSVVCAGGS+IISP G +LAGPNY GEALI+ADLDLG+IARAKFDFD
Sbjct: 260 EYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGDIARAKFDFD 319

Query: 313 VVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDS 348
           VVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 320 VVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]
BLAST of MELO3C025585 vs. TAIR 10
Match: AT3G44300.1 (nitrilase 2 )

HSP 1 Score: 468.4 bits (1204), Expect = 5.1e-132
Identity = 217/327 (66.36%), Postives = 264/327 (80.73%), Query Frame = 0 

Query: 24  SSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGV 83
           +S   VRAT+VQAST++ DTPATL+KA + + EAA  GS+LVVFPEAF+GGYPRG  FG+
Sbjct: 13  ASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGL 72

Query: 84  SIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLF 143
            +G    +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LF
Sbjct: 73  GVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALF 132

Query: 144 FDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMY 203
           F PQG++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+Y
Sbjct: 133 FSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALY 192

Query: 204 AKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEEL 263
           AKGIE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++ 
Sbjct: 193 AKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDK 252

Query: 264 TPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVL 323
            PDS+V  GGS IISP G +LAGPN++ E LI+ADLDLG++ARAK  FD VGHY+RP+VL
Sbjct: 253 EPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVL 312

Query: 324 SLVVRDHPAIPVTFTSTSTKAEDSCKK 351
            L V +HP  PVTF S   KAED   K
Sbjct: 313 HLTVNEHPKKPVTFISKVEKAEDDSNK 339

[Close]
BLAST of MELO3C025585 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-131
Identity = 217/331 (65.56%), Postives = 266/331 (80.36%), Query Frame = 0 

Query: 20  MGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGS 79
           +G D S  TVR T+VQ+ST++ DTPATLDKAE+ + EAA  G++LV+FPEAF+GGYPRG 
Sbjct: 17  IGVDPS-STVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGF 76

Query: 80  NFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYC 139
            FG+++G    +G++EFR YHA+AI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYC
Sbjct: 77  RFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYC 136

Query: 140 TVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLR 199
           T LFF PQG++LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL R
Sbjct: 137 TALFFSPQGQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYR 196

Query: 200 TAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGT 259
           TA+YAKGIEIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+  
Sbjct: 197 TALYAKGIEIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDI 256

Query: 260 EEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYAR 319
            +    D  V  GGS IISP G +LAGPNY+ E L++ADLDLG+IARAK  FDVVGHY++
Sbjct: 257 VDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSK 316

Query: 320 PEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 351
           P++ +L V +HP  PVTF +   KAED   K
Sbjct: 317 PDIFNLTVNEHPKKPVTFMTKVEKAEDESNK 346

[Close]
BLAST of MELO3C025585 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 452.6 bits (1163), Expect = 2.9e-127
Identity = 208/323 (64.40%), Postives = 260/323 (80.50%), Query Frame = 0 

Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDS 267
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 268 VVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVV 327
           +V  GGS IISP G +LAGPN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 328 RDHPAIPVTFTSTSTKAEDSCKK 351
            +HP   VTF +   KAED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

[Close]
BLAST of MELO3C025585 vs. TAIR 10
Match: AT3G44310.3 (nitrilase 1 )

HSP 1 Score: 452.6 bits (1163), Expect = 2.9e-127
Identity = 208/323 (64.40%), Postives = 260/323 (80.50%), Query Frame = 0 

Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDS 267
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 268 VVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVV 327
           +V  GGS IISP G +LAGPN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 328 RDHPAIPVTFTSTSTKAEDSCKK 351
            +HP   VTF +   KAED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

The following BLAST results are available for this feature:
BLAST of MELO3C025585 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q429659.2e-17987.39Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N... [more]
Q429663.5e-17887.11Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N... [more]
Q5QGZ81.1e-17685.67Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ... [more]
Q3LRV47.3e-17686.32Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ... [more]
Q6H8494.9e-16480.80Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX... [more]
BLAST of MELO3C025585 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008463648.11.3e-201100.00PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA003... [more]
XP_004139500.18.5e-19898.00bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hyp... [more]
XP_023520715.14.4e-19496.29bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo][more]
KAG6583706.12.2e-19395.71Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma ... [more]
XP_038894429.12.8e-19395.73bifunctional nitrilase/nitrile hydratase NIT4A isoform X1 [Benincasa hispida][more]
BLAST of MELO3C025585 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SY836.2e-202100.00Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CLB46.2e-202100.00bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LVI04.1e-19898.00CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16836... [more]
A0A6J1I7W01.5e-19295.43bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EHI62.6e-19295.42bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=... [more]
BLAST of MELO3C025585 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G22300.17.0e-15878.81nitrilase 4 [more]
AT3G44300.15.1e-13266.36nitrilase 2 [more]
AT3G44320.11.5e-13165.56nitrilase 3 [more]
AT3G44310.12.9e-12764.40nitrilase 1 [more]
AT3G44310.32.9e-12764.40nitrilase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 30..308
e-value: 9.7E-70
score: 234.9
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 29..301
score: 58.307804
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 18..313
e-value: 1.3E-86
score: 292.1
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 28..327
NoneNo IPR availablePANTHERPTHR46044:SF1NITRILASEcoord: 13..343
IPR044149Nitrilase/Cyanide hydratasePANTHERPTHR46044NITRILASEcoord: 13..343
IPR044149Nitrilase/Cyanide hydrataseCDDcd07564nitrilases_CHscoord: 29..329
e-value: 8.18679E-162
score: 452.327
IPR000132Nitrilase/cyanide hydratase, conserved sitePROSITEPS00921NITRIL_CHT_2coord: 186..199
IPR000132Nitrilase/cyanide hydratase, conserved sitePROSITEPS00920NITRIL_CHT_1coord: 61..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C025585.1MELO3C025585.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0003824 catalytic activity