MELO3C021228 (gene) Melon (DHL92) v4

Overview


Name	MELO3C021228
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	tRNA pseudouridine synthase
Location	chr11: 28813399 .. 28815334 (+)
RNA-Seq Expression	MELO3C021228
Synteny	MELO3C021228

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAAACTAGGCTGGCAATATCAGAGGTCACCACCCACCATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTCGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTCTGATGTGATTTTATGGAGTTGGGTTAAAACACATCATTGGAATACAATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGTAAAGTTTTAAGGTTCTTTAGCCAGTTATTTGTTTGGTTAGAATTACCAATTTAGTCTATAAACTTTCTGTCTATTTGGTCATCTTTCAAAAGTGTTCAATAGGTCATTGCACTTCAATCTTGTACCATATAGGACGTCGAACTTTAAAATGTTTTGAAAGGTTCTTAAACTTTCAAATTCATGTTTATTAAATCTCTTATTTTCGGTTTTGTGTTTACATTGACATAATATTCAAATTTATATATTGTAGGTCAATTAGTTAAAAAAAATGAATTTGCCAAGGACTTATTAGGCACAAAATTGATAGTTTAAGGGTCTATCAAACATTTTTAACTGATCGAGATCCATTTGATACATTTGCAAGTTCAAGACCAATAGATTCAAACATTAGTCTATGGACTAAATTTACAGTCTAATCAGCTTCTTTTGTAATTTTTTTTATCCCTATGATGTGTCTGCTTCTCAGGTCTAAAGTTGCTAATTCTGTATGCCCTTCTTTTGATCATCTGTGTGAGTGTGAATCATTCCGGTTTCAGCTTTTTACAAAATCTATGCAACTAAATGCTTTCTTTTGTTCAGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTAATTTTTGTTATATCTATCTTTTCAGTCAAAAGTCCCTTCTCTGATTGTTTTATTTTTACTTATGCTGGTTGCTAAAATGGCTTTATAGTCCTGGAAGCATAGATTTAAGTTTGTAAAATCGGTTTCATGCAAGGATTTTCTCTTGTGTGTTTTCAGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGAATTAACCTTACACTTATGCCTTGAGAATAATCTGCGTTGCGGGGTGTCTCATCAACTTAGGAGGCTGAGATATTAGATAAGCAGTGTAGTTTAGTTAGCAAAATTTTGGATTAATTAGTCTTTGTTAATTTAGTATATTTGTGTTAGTTTGTTATTTGTCTTTTCATAAGCCTATAGCCACTTTTAGTACGCTGTATAGGAACTCTAGAGGCTTTGAACATTATTATTGCAATACAAGGTATTTTAAGAGGGTTTCTCTCAATTTTGGAGAATTAAAGGTTATTTCCAAGAATGGGTT

mRNA sequence

ATTAAACTAGGCTGGCAATATCAGAGGTCACCACCCACCATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTCGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTCTGATGTGATTTTATGGAGTTGGGTTAAAACACATCATTGGAATACAATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGAATTAACCTTACACTTATGCCTTGAGAATAATCTGCGTTGCGGGGTGTCTCATCAACTTAGGAGGCTGAGATATTAGATAAGCAGTGTAGTTTAGTTAGCAAAATTTTGGATTAATTAGTCTTTGTTAATTTAGTATATTTGTGTTAGTTTGTTATTTGTCTTTTCATAAGCCTATAGCCACTTTTAGTACGCTGTATAGGAACTCTAGAGGCTTTGAACATTATTATTGCAATACAAGGTATTTTAAGAGGGTTTCTCTCAATTTTGGAGAATTAAAGGTTATTTCCAAGAATGGGTT

Coding sequence (CDS)

ATGCCTGGGGTCAGGTCTGATGTGATTTTATGGAGTTGGGTTAAAACACATCATTGGAATACAATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGA

Protein sequence

MPGVRSDVILWSWVKTHHWNTMLAVAYDKLRFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY

Homology

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BLAST of MELO3C021228 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-37
Identity = 77/196 (39.29%), Postives = 123/196 (62.76%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHF    +Y  L  +H +LNGLLP DIR++ + P +P FH+++S + K YHY ++   
Sbjct: 61  QVAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNK 120

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVV-NPVKVIFRFDV 152
           +MDPFQR Y++H   K++   +K+AA  F G HDF++F N AH      NPV+ ++R D+
Sbjct: 121 VMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDI 180

Query: 153 IEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGV 212
                 ++LE EG GFLY+ VRN+VG L+ +       E + +I A+++R++ +  A   
Sbjct: 181 KPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA--- 240

Query: 213 PPQGLCLVSINYKEDH 228
           PP+GL L+ + Y+ ++
Sbjct: 241 PPEGLFLIQVFYENEN 252

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BLAST of MELO3C021228 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.2e-33
Identity = 81/193 (41.97%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 31  RFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYN 90
           R QV +FVT       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +YN
Sbjct: 60  RGQVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYN 119

Query: 91  GTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFD 150
             +  PFQR Y+ H    L+  AM+ AA + +G+HDFS F   A    V + V+ + R D
Sbjct: 120 HPVRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTF--QAAGRPVQSAVRTLTRAD 179

Query: 151 VIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASG 210
           +     LV+L     GFLY  VR M G L+++G E L PE +P I+AS DR   A+    
Sbjct: 180 IEAQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPT 239

Query: 211 VPPQGLCLVSINY 224
           VPP GLCL  + Y
Sbjct: 240 VPPHGLCLEEVEY 247

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BLAST of MELO3C021228 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-30
Identity = 84/192 (43.75%), Postives = 106/192 (55.21%), Query Frame = 0


Query: 32  FQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNG 91
           +QVA+F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I N 
Sbjct: 55  YQVANFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNR 114

Query: 92  TIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDV 151
               P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  N+ VNPVK IF   V
Sbjct: 115 QFPMPTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTV 174

Query: 152 IEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGV 211
            + G L+++EVE  GFLY  VR +VG LV +G   +    +P I+ S+DR    K     
Sbjct: 175 EKEGDLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITA 234

Query: 212 PPQGLCLVSINY 224
           PPQGL LV I Y
Sbjct: 235 PPQGLYLVRIVY 240

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BLAST of MELO3C021228 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-30
Identity = 73/177 (41.24%), Postives = 103/177 (58.19%), Query Frame = 0


Query: 47  ESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCV 106
           E    ALN LLP DI+V +      DFHARFS ++K Y Y IY+  +M PF R Y YH  
Sbjct: 76  EKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHRVMSPFHRYYCYHEP 135

Query: 107 YKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQLEVEGSG 166
            +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+IE   ++ L + G+G
Sbjct: 136 RRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADIIEDAPVINLYLRGNG 195

Query: 167 FLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLVSINY 224
           FLY  VR + G L+ IG   + PE + KI+ S++R      A   PPQGLCL+ + Y
Sbjct: 196 FLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---PPQGLCLMEVEY 247

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BLAST of MELO3C021228 vs. ExPASy Swiss-Prot
Match: Q3A3B5 (tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=truA PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 5.7e-29
Identity = 79/206 (38.35%), Postives = 111/206 (53.88%), Query Frame = 0


Query: 31  RFQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYN 90
           R  VAHFVTP     + +    +N  LP  I VR+ +    DFHAR+S + K Y Y IY 
Sbjct: 58  RGMVAHFVTPQPL-PMAAYREGVNRFLPAQIAVRDAALMPDDFHARYSAVGKWYRYSIYR 117

Query: 91  GTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFD 150
             +  P    +++H    L+  AM++AA   VG+HDF+AF  +    R    V+ +F  D
Sbjct: 118 SPVRSPLACRFSWHVRTVLDLQAMRQAARLLVGRHDFAAFRASGCAARTT--VREVFSVD 177

Query: 151 VIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASG 210
           +++    V ++V GSGFL   VR MVG LV++G+ A PPE V  +LA   RK+     SG
Sbjct: 178 IVDAEEWVYVDVRGSGFLRNMVRIMVGTLVEVGRGARPPEEVGAMLAGGCRKQ-----SG 237

Query: 211 V--PPQGLCLVSINYKEDHLKLPSDC 235
           +  P QGLCL+ + Y       P DC
Sbjct: 238 ITAPAQGLCLMEVAY------APQDC 249

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BLAST of MELO3C021228 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 456.1 bits (1172), Expect = 2.0e-124
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of MELO3C021228 vs. NCBI nr
Match: XP_008458280.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo])

HSP 1 Score: 456.1 bits (1172), Expect = 2.0e-124
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 84  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 143

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 144 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 203

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 204 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 263

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 264 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of MELO3C021228 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 444.5 bits (1142), Expect = 6.1e-121
Identity = 214/221 (96.83%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 135 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 194

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 195 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 254

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 255 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 314

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 315 PQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

[Close]

BLAST of MELO3C021228 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 435.6 bits (1119), Expect = 2.8e-118
Identity = 206/221 (93.21%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHF+TPFNYDSLESVHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG 
Sbjct: 114 QVAHFITPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGA 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           I+DPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 174 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           +MG LVQLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DR+ELAKYASGVP
Sbjct: 234 DMGPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTS+GR+RSV KCK+PFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

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BLAST of MELO3C021228 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 427.6 bits (1098), Expect = 7.7e-116
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLG 241
           PQGLCLVSINYKEDHLKLPSDCPGTSLG
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of MELO3C021228 vs. ExPASy TrEMBL
Match: A0A1S3C7H4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 9.8e-125
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 84  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 143

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 144 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 203

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 204 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 263

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 264 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of MELO3C021228 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 9.8e-125
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of MELO3C021228 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.7e-116
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT
Sbjct: 114 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI
Sbjct: 174 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP
Sbjct: 234 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLG 241
           PQGLCLVSINYKEDHLKLPSDCPGTSLG
Sbjct: 294 PQGLCLVSINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of MELO3C021228 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.7e-114
Identity = 204/214 (95.33%), Postives = 209/214 (97.66%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GT
Sbjct: 115 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 174

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI
Sbjct: 175 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 234

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVP
Sbjct: 235 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 294

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVG 247
           PQGLCLVSINYKEDHLKLPSDCPGTS G   ++G
Sbjct: 295 PQGLCLVSINYKEDHLKLPSDCPGTSFGCPNNLG 328

[Close]

BLAST of MELO3C021228 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.9e-112
Identity = 193/221 (87.33%), Postives = 207/221 (93.67%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHF+TPFNYDSL+ +HAALNGLLP DIRVREMSPA PDFHARFSTLSKVYHYKIYN  
Sbjct: 114 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDA 173

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
           I+DPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHN+RVVNPVKVIFRFDVI
Sbjct: 174 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 233

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 212
           EMGAL+QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDRKELAKY + +P
Sbjct: 234 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 293

Query: 213 PQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
           PQGLCLV INYKEDHLKLPSDCPGTS GRS SV KCK+PFY
Sbjct: 294 PQGLCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 334

[Close]

BLAST of MELO3C021228 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 322.8 bits (826), Expect = 2.5e-88
Identity = 156/222 (70.27%), Postives = 179/222 (80.63%), Query Frame = 0


Query: 33  QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 92
           QVAHFVTPFNY SL+S HAALNGLLP DIRVRE+S A P+FHARFS  SKVY Y+IYN T
Sbjct: 102 QVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDT 161

Query: 93  IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 152
            MDPFQR + YHC YKLN + M+EAAN FVGKHDFSAF NA   + V +P+K I RFDVI
Sbjct: 162 FMDPFQRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVI 221

Query: 153 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYAS-GV 212
           +MG+L+QLEVEGSGFLYRQVRNMV LL+QIGKEAL  ++VP IL ++DR+ LAKY S   
Sbjct: 222 QMGSLLQLEVEGSGFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPA 281

Query: 213 PPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 254
            P GLCLVS+ YKEDHLKLP DCP TS GR  ++ KCK+PFY
Sbjct: 282 SPHGLCLVSVKYKEDHLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of MELO3C021228 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.5e-11
Identity = 55/168 (32.74%), Postives = 75/168 (44.64%), Query Frame = 0


Query: 53  LNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCVYKLNYA 112
           LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +                   LN +
Sbjct: 191 LNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWR----------------QNLNLS 250

Query: 113 AMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI------EMGALVQLEVEGSG 172
           AM  A   F+G+HDF  F      N      +V F F+V       E   L    + GS 
Sbjct: 251 AMDFAGKKFIGEHDFRNFCKMDVANVHCYTRRVTF-FEVSPCQNSHEGDQLCTFTMRGSA 310

Query: 173 FLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKY--ASGVP 213
           FL+ Q+R MV +L  IG+     +V+  +L ++      +Y  AS +P
Sbjct: 311 FLWHQIRCMVAVLFMIGQGVESVDVIDTLLDTKKTPRKPQYLLASEIP 341

The following BLAST results are available for this feature:

BLAST of MELO3C021228 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	2.5e-37	39.29	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	2.2e-33	41.97	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
Q8R7Y7	1.4e-30	43.75	tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen...	[more]
A4J151	1.4e-30	41.24	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
Q3A3B5	5.7e-29	38.35	tRNA pseudouridine synthase A OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC...	[more]

BLAST of MELO3C021228 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458279.1	2.0e-124	100.00	PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo]	[more]
XP_008458280.1	2.0e-124	100.00	PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo]	[more]
XP_004138518.2	6.1e-121	96.83	uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ...	[more]
XP_038875516.1	2.8e-118	93.21	tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida]	[more]
TYK02989.1	7.7e-116	100.00	protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa]	[more]

BLAST of MELO3C021228 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7H4	9.8e-125	100.00	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A1S3C7L6	9.8e-125	100.00	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A5D3BY40	3.7e-116	100.00	Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0KB67	2.7e-114	95.33	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1	[more]
A0A6J1H621	1.9e-112	87.33	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]

BLAST of MELO3C021228 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	2.5e-88	70.27	Pseudouridine synthase family protein	[more]
AT1G34150.1	2.5e-11	32.74	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 117..223 e-value: 2.0E-25 score: 89.4
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 80..222 e-value: 6.4E-40 score: 138.1
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 30..79 e-value: 1.1E-5 score: 27.6
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 32..233
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 32..233
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 33..223 e-value: 7.26746E-79 score: 235.831
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 32..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C021228.1	MELO3C021228.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding